Mercurial > repos > jjohnson > mothur_toolsuite
annotate mothur/tools/mothur/make.contigs.xml @ 31:a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
make.contigs.xml.patch:# make.contigs.xml.patch
make.contigs.xml.patch:#
make.contigs.xml.patch:# 1. Fix cosmetic typo in <description> (forard -> forward)
make.contigs.xml.patch:# 2. Address error due to having 'mismatch' as the name for both an input and an output parameter:
make.contigs.xml.patch:# rename output parameter to 'cmismatch'
make.contigs.xml.patch:# 3. Remove 'threshold' parameter: make.contigs in mothur doesn't support a 'threshold' parameter
metagenomics.py.patch:# metagenomics.py.patch
metagenomics.py.patch:#
metagenomics.py.patch:# 1. Groups class: names were being taken from the wrong field (affected shhh.flows tool)
metagenomics.py.patch:# 2. Axes class: make 'sniff' method more sensitive to try and restrict arbitrary tabular
metagenomics.py.patch:# data uploads being sniffed as this type
mothur_wrapper.py.patch:# mothur_wrapper.py.patch
mothur_wrapper.py.patch:#
mothur_wrapper.py.patch:# 1. Update 'cmd_dict' settings for shhh.flows and shhh.seqs (otherwise these functions will
mothur_wrapper.py.patch:# fail on execution)
mothur_wrapper.py.patch:# 2. Fix add_option calls defining '--match' and '--mismatch' command line options (otherwise
mothur_wrapper.py.patch:# syntax error causes immediate failure)
screen.seqs.xml.patch:# screen.seqs.xml.patch
screen.seqs.xml.patch:#
screen.seqs.xml.patch:# Replace pattern for align.report output file in definiting of 'results' parameter in
screen.seqs.xml.patch:# <command> section (otherwise output_alignreport data item is empty).
shhh.flows.xml.patch:# shhh.flows.xml.patch
shhh.flows.xml.patch:#
shhh.flows.xml.patch:# Replace 'format_source' with 'format' for output parameters (otherwise formats are not
shhh.flows.xml.patch:# correctly assigned to output datasets)
shhh.seqs.xml.patch:# shhh.seqs.xml.patch
shhh.seqs.xml.patch:#
shhh.seqs.xml.patch:# 1. Fix patterns in --result (in <command> section) for shhh_seqs.fasta and shhh_seqs.names
shhh.seqs.xml.patch:# output files (otherwise files are not collected and associated data items are empty)
shhh.seqs.xml.patch:# 2. Replace 'format_source' with 'format' for output parameters (otherwise formats are not
shhh.seqs.xml.patch:# correctly assigned to output datasets)
trim.flows.xml.patch:# trim.flows.xml.patch
trim.flows.xml.patch:#
trim.flows.xml.patch:# Remove erroneous space from --result definition in <command> section (otherwise causes tool
trim.flows.xml.patch:# failure)
trim.seqs.xml.patch:# trim.seqs.xml.patch
trim.seqs.xml.patch:#
trim.seqs.xml.patch:# 1. Remove reference to undefined 'oligo.allvalues' varible in <command> section (otherwise
trim.seqs.xml.patch:# causes failure on execution)
trim.seqs.xml.patch:# 2. Fix format for input parameter 'names' (format should be 'names' not 'name')
trim.seqs.xml.patch:# 3. Add output parameter 'scrap_names' (to ensure consistent collection of all outputs)
trim.seqs.xml.patch:# 4. Update --result definition in <command> section to collect both trim.names and scrap.names
author | Jim Johnson <jj@umn.edu> |
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date | Tue, 30 Jul 2013 09:26:31 -0500 |
parents | 49058b1f8d3f |
children | 95d75b35e4d2 |
rev | line source |
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1 <tool id="mothur_make_contigs" name="Make.contigs" version="1.26.0"> |
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2 <description>Aligns paired forward and reverse fastq files to contigs as fasta and quality</description> |
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3 <command interpreter="python"> |
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4 mothur_wrapper.py |
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5 --cmd='make.contigs' |
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6 --result='^mothur.\S+\.logfile$:'$logfile,'^\S+\.contigs\.fasta$:'$fasta,'^\S+\.contigs\.qual$:'$qual,'^\S+\.contigs\.mismatch$:'$cmismatch |
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7 --outputdir='$logfile.extra_files_path' |
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8 --ffastq=$ffastq |
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9 --rfastq=$rfastq |
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10 --align=$align |
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11 #if $oligo.add == "yes": |
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12 --oligos=$oligo.oligos |
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13 #if $oligo.bdiffs.__str__ != '' and int($oligo.bdiffs.__str__) > 0: |
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14 --bdiffs=$oligo.bdiffs |
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15 #end if |
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16 #if $oligo.pdiffs.__str__ != '' and int($oligo.pdiffs.__str__) > 0: |
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17 --pdiffs=$oligo.pdiffs |
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18 #end if |
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19 #if $oligo.tdiffs.__str__ != '' and int($oligo.tdiffs.__str__) > 0: |
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20 --tdiffs=$oligo.tdiffs |
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21 #end if |
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22 #end if |
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23 |
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24 #if $match.__str__ != '': |
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25 --match=$match |
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26 #end if |
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27 #if $mismatch.__str__ != '': |
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28 --mismatch=$mismatch |
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29 #end if |
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30 #if $gapopen.__str__ != '': |
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31 --gapopen=$gapopen |
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32 #end if |
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33 #if $gapextend.__str__ != '': |
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34 --gapextend=$gapextend |
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35 #end if |
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36 ###if $threshold.__str__ != '': |
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37 ## --threshold=$threshold |
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38 ###end if |
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39 --processors=8 |
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40 </command> |
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41 <inputs> |
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42 <param name="ffastq" type="data" format="fastq" label="ffastq - Forward Fastq Sequence file"/> |
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43 <param name="rfastq" type="data" format="fastq" label="rfastq - Reverse Fastq Sequence file"/> |
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44 <param name="align" type="select" label="align - Select a pairwise alignment method" help=""> |
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45 <option value="gotoh" selected="true">gotoh (default)</option> |
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46 <option value="needleman">needleman</option> |
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47 </param> |
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48 <conditional name="oligo"> |
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49 <param name="add" type="select" label="Trim with an oligos file?" help=""> |
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50 <option value="no">no</option> |
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51 <option value="yes">yes</option> |
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52 </param> |
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53 <when value="no"/> |
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54 <when value="yes"> |
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55 <param name="oligos" type="data" format="oligos" optional="true" label="oligos - barcodes and primers" |
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56 help="a file that can contain the sequences of the forward and reverse primers and barcodes and their sample identifier. |
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57 Each line of the oligos file can start with the key words "forward", "reverse", |
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58 and "barcode" or it can start with a "#" to tell mothur to ignore that line of the oligos file. "/> |
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59 <param name="bdiffs" type="integer" value="0" label="bdiffs - number of differences to allow in the barcode (default 0)"> |
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60 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
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61 </param> |
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62 <param name="pdiffs" type="integer" value="0" label="pdiffs - number of differences to allow in the primer (default 0)"> |
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63 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
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64 </param> |
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65 <param name="tdiffs" type="integer" value="0" label="tdiffs - total number of differences to allow in primer and barcode (default 0)"> |
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66 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
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67 </param> |
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68 </when> |
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69 </conditional> |
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70 <param name="match" type="integer" value="1" optional="true" label="match - Pairwise alignment reward for a match"/> |
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71 <param name="mismatch" type="integer" value="-1" optional="true" label="mismatch - Pairwise alignment penalty for a mismatch"/> |
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72 <param name="gapopen" type="integer" value="-2" optional="true" label="gapopen - Pairwise alignment penalty for opening a gap"/> |
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73 <param name="gapextend" type="integer" value="-1" optional="true" label="gapextend - Pairwise alignment penalty for extending a gap"/> |
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74 <!-- make.contigs doesn't support "threshold" option, see http://www.mothur.org/wiki/Make.contigs |
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75 <param name="threshold" type="integer" value="40" optional="true" label="threshold - for quality scores" |
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76 help="When we are merging the overlapping regions, in the case where we are trying to decide whether to keep a base or remove it because the base is compared to a gap in the other fragment, if the base has a quality score below the threshold we eliminate it."> |
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77 <validator type="in_range" message="threshold can't be negative" min="0"/> |
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78 </param> |
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79 --> |
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80 </inputs> |
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81 <outputs> |
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82 <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" /> |
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83 <data format="fasta" name="fasta" label="${tool.name} on ${on_string}: contigs.fasta"/> |
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84 <data format="qual" name="qual" label="${tool.name} on ${on_string}: contigs.qual"/> |
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85 <data format="txt" name="cmismatch" label="${tool.name} on ${on_string}: contigs.mismatch"/> |
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86 </outputs> |
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87 <requirements> |
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88 <requirement type="package" version="1.27">mothur</requirement> |
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89 </requirements> |
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90 <tests> |
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91 </tests> |
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92 <help> |
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93 **Mothur Overview** |
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94 |
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95 Mothur_, initiated by Dr. Patrick Schloss and his software development team |
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96 in the Department of Microbiology and Immunology at The University of Michigan, |
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97 provides bioinformatics for the microbial ecology community. |
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98 |
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99 .. _Mothur: http://www.mothur.org/wiki/Main_Page |
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100 |
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101 **Command Documenation** |
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102 |
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103 The make.contigs_ command reads a forward fastq file and a reverse fastq file and outputs new fasta and quality files. |
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104 |
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105 .. _make.contigs: http://www.mothur.org/wiki/Make.contigs |
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106 |
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107 </help> |
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108 </tool> |