Mercurial > repos > jjohnson > mothur_toolsuite
annotate mothur/tools/mothur/trim.seqs.xml @ 31:a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
make.contigs.xml.patch:# make.contigs.xml.patch
make.contigs.xml.patch:#
make.contigs.xml.patch:# 1. Fix cosmetic typo in <description> (forard -> forward)
make.contigs.xml.patch:# 2. Address error due to having 'mismatch' as the name for both an input and an output parameter:
make.contigs.xml.patch:# rename output parameter to 'cmismatch'
make.contigs.xml.patch:# 3. Remove 'threshold' parameter: make.contigs in mothur doesn't support a 'threshold' parameter
metagenomics.py.patch:# metagenomics.py.patch
metagenomics.py.patch:#
metagenomics.py.patch:# 1. Groups class: names were being taken from the wrong field (affected shhh.flows tool)
metagenomics.py.patch:# 2. Axes class: make 'sniff' method more sensitive to try and restrict arbitrary tabular
metagenomics.py.patch:# data uploads being sniffed as this type
mothur_wrapper.py.patch:# mothur_wrapper.py.patch
mothur_wrapper.py.patch:#
mothur_wrapper.py.patch:# 1. Update 'cmd_dict' settings for shhh.flows and shhh.seqs (otherwise these functions will
mothur_wrapper.py.patch:# fail on execution)
mothur_wrapper.py.patch:# 2. Fix add_option calls defining '--match' and '--mismatch' command line options (otherwise
mothur_wrapper.py.patch:# syntax error causes immediate failure)
screen.seqs.xml.patch:# screen.seqs.xml.patch
screen.seqs.xml.patch:#
screen.seqs.xml.patch:# Replace pattern for align.report output file in definiting of 'results' parameter in
screen.seqs.xml.patch:# <command> section (otherwise output_alignreport data item is empty).
shhh.flows.xml.patch:# shhh.flows.xml.patch
shhh.flows.xml.patch:#
shhh.flows.xml.patch:# Replace 'format_source' with 'format' for output parameters (otherwise formats are not
shhh.flows.xml.patch:# correctly assigned to output datasets)
shhh.seqs.xml.patch:# shhh.seqs.xml.patch
shhh.seqs.xml.patch:#
shhh.seqs.xml.patch:# 1. Fix patterns in --result (in <command> section) for shhh_seqs.fasta and shhh_seqs.names
shhh.seqs.xml.patch:# output files (otherwise files are not collected and associated data items are empty)
shhh.seqs.xml.patch:# 2. Replace 'format_source' with 'format' for output parameters (otherwise formats are not
shhh.seqs.xml.patch:# correctly assigned to output datasets)
trim.flows.xml.patch:# trim.flows.xml.patch
trim.flows.xml.patch:#
trim.flows.xml.patch:# Remove erroneous space from --result definition in <command> section (otherwise causes tool
trim.flows.xml.patch:# failure)
trim.seqs.xml.patch:# trim.seqs.xml.patch
trim.seqs.xml.patch:#
trim.seqs.xml.patch:# 1. Remove reference to undefined 'oligo.allvalues' varible in <command> section (otherwise
trim.seqs.xml.patch:# causes failure on execution)
trim.seqs.xml.patch:# 2. Fix format for input parameter 'names' (format should be 'names' not 'name')
trim.seqs.xml.patch:# 3. Add output parameter 'scrap_names' (to ensure consistent collection of all outputs)
trim.seqs.xml.patch:# 4. Update --result definition in <command> section to collect both trim.names and scrap.names
author | Jim Johnson <jj@umn.edu> |
---|---|
date | Tue, 30 Jul 2013 09:26:31 -0500 |
parents | 49058b1f8d3f |
children | 95d75b35e4d2 |
rev | line source |
---|---|
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
1 <tool id="mothur_trim_seqs" name="Trim.seqs" version="1.24.0" force_history_refresh="True"> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
2 <description>Trim sequences - primers, barcodes, quality</description> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
3 <command interpreter="python"> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
4 mothur_wrapper.py |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
5 --cmd='trim.seqs' |
31
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
6 --result='^mothur.\S+\.logfile$:'$logfile,'^\S+\.trim\.fasta$:'$trim_fasta,'^\S+\.trim\.names$:'$trim_names,'^\S+\.trim\.qual$:'$trim_qual,'^\S+\.scrap\.fasta$:'$scrap_fasta,'^\S+\.scrap\.names$:'$scrap_names,'^\S+\.scrap\.qual$:'$scrap_qual,'^\S+\.groups$:'$groups_file |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
7 --outputdir='$logfile.extra_files_path' |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
8 #if int($minlength.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
9 --minlength=$minlength |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
10 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
11 #if int($maxlength.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
12 --maxlength=$maxlength |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
13 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
14 #if int($maxambig.__str__) >= 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
15 --maxambig=$maxambig |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
16 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
17 #if int($maxhomop.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
18 --maxhomop=$maxhomop |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
19 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
20 #if int($keepfirst.__str__) > 0: |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
21 --keepfirst=$keepfirst |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
22 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
23 #if int($removelast.__str__) > 0: |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
24 --removelast=$removelast |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
25 #end if |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
26 #if $oligo.add == "yes": |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
27 --oligos=$oligo.oligos |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
28 #if $oligo.bdiffs.__str__ != '' and int($oligo.bdiffs.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
29 --bdiffs=$oligo.bdiffs |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
30 #end if |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
31 #if $oligo.pdiffs.__str__ != '' and int($oligo.pdiffs.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
32 --pdiffs=$oligo.pdiffs |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
33 #end if |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
34 #if $oligo.tdiffs.__str__ != '' and int($oligo.tdiffs.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
35 --tdiffs=$oligo.tdiffs |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
36 #end if |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
37 #if $oligo.ldiffs.__str__ != '' and int($oligo.ldiffs.__str__) > 0: |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
38 --ldiffs=$oligo.ldiffs |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
39 #end if |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
40 #if $oligo.sdiffs.__str__ != '' and int($oligo.sdiffs.__str__) > 0: |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
41 --sdiffs=$oligo.sdiffs |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
42 #end if |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
43 $oligo.keepforward |
31
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
44 ##$oligo.allvalues |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
45 #if $oligo.allfiles == True: |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
46 --datasetid='$logfile.id' --new_file_path='$__new_file_path__' |
4 | 47 --new_datasets='^\S+?\.(\S+\.fasta)$:${fasta.ext}','^\S+?\.(\S+\.groups)$:groups' |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
48 #end if |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
49 #end if |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
50 #if $qual.add == "yes": |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
51 --qfile=$qual.qfile |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
52 #if int($qual.qaverage.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
53 --qaverage=$qual.qaverage |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
54 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
55 #if int($qual.qthreshold.__str__) > 0: |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
56 --qthreshold=$qual.qthreshold |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
57 #end if |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
58 #if int($qual.qwindowaverage.__str__) > 0: |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
59 --qwindowaverage=$qual.qwindowaverage |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
60 #end if |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
61 #if int($qual.qwindowsize.__str__) > 0: |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
62 --qwindowsize=$qual.qwindowsize |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
63 #end if |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
64 #if int($qual.rollaverage.__str__) > 0: |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
65 --rollaverage=$qual.rollaverage |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
66 #end if |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
67 #if int($qual.qstepsize.__str__) > 0: |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
68 --qstepsize=$qual.qstepsize |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
69 #end if |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
70 $qual.qtrim |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
71 #end if |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
72 $flip |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
73 --fasta=$fasta |
7 | 74 #if $names.__str__ != "None" and len($names.__str__) > 0: |
75 --name=$names | |
76 #end if | |
13
4f797d3eee3a
Change default processor counts to 8, mothur_wrapper.py can overide processors allocated with ENVIRONMENT variable MOTHUR_MAX_PROCESSORS
Jim Johnson <jj@umn.edu>
parents:
8
diff
changeset
|
77 --processors=8 |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
78 </command> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
79 <inputs> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
80 <param name="fasta" type="data" format="fasta" label="fasta - Sequences"/> |
31
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
81 <param name="names" type="data" format="names" optional="true" label="name - Sequence representative name list"/> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
82 <param name="minlength" type="integer" value="0" label="minlength - Minimum Sequence Length (default 0, ignored if < 1 )"/> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
83 <param name="maxlength" type="integer" value="0" label="maxlength - Maximum Sequence Length (default 0, ignored if < 1)"/> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
84 <param name="maxambig" type="integer" value="-1" label="maxambig - Maximum ambiguous bases (default -1, ignored if < 0)"/> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
85 <param name="maxhomop" type="integer" value="0" label="maxhomop - Maximum homopolymers (default 0, ignored if < 1)"/> |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
86 <param name="keepfirst" type="integer" value="0" label="keepfirst - ignored if < 0)" |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
87 help="trims the sequence to the first keepfirst number of bases after the barcode or primers are removed, before the sequence is checked to see if it meets the other requirements"/> |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
88 <param name="removelast" type="integer" value="0" label="removelast - ignored if < 0)" |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
89 help="removes the last removelast number of bases after the barcode or primers are removed, before the sequence is checked to see if it meets the other requirements."/> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
90 <conditional name="oligo"> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
91 <param name="add" type="select" label="Trim with an oligos file?" help=""> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
92 <option value="no">no</option> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
93 <option value="yes">yes</option> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
94 </param> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
95 <when value="no"/> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
96 <when value="yes"> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
97 <param name="oligos" type="data" format="oligos" label="oligos - barcodes and primers"/> |
24
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
98 <param name="bdiffs" type="integer" value="0" label="bdiffs - number of differences to allow in the barcode (default 0)"> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
99 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
100 </param> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
101 <param name="pdiffs" type="integer" value="0" label="pdiffs - number of differences to allow in the primer (default 0)"> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
102 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
103 </param> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
104 <param name="tdiffs" type="integer" value="0" label="tdiffs - total number of differences to allow in primer and barcode (default 0)"> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
105 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
106 </param> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
107 <param name="ldiffs" type="integer" value="0" optional="true" label="ldiffs - total number of differences to allow in linker sequence (default 0)"> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
108 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
109 </param> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
110 <param name="sdiffs" type="integer" value="0" optional="true" label="sdiffs - total number of differences to allow in spacer sequence (default 0)"> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
111 <validator type="in_range" message="Number of differences can't be negative" min="0"/> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
112 </param> |
09740be2bc9c
Mothur - updates for Mothur version 1.24
Jim Johnson <jj@umn.edu>
parents:
13
diff
changeset
|
113 <param name="keepforward" type="boolean" truevalue="--keepforward=true" falsevalue="" checked="false" label="keepforward - keep the primer"/> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
114 <param name="allfiles" type="boolean" truevalue="--allfiles=true" falsevalue="" checked="false" label="allfiles - separate into file per barcode"/> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
115 </when> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
116 </conditional> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
117 <conditional name="qual"> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
118 <param name="add" type="select" label="Trim based on a quality file?" help=""> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
119 <option value="no">no</option> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
120 <option value="yes">yes</option> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
121 </param> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
122 <when value="no"/> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
123 <when value="yes"> |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
124 <param name="qfile" type="data" format="qual454" label="qfile - 454 quality file"/> |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
125 <param name="qaverage" type="integer" value="0" label="qaverage - remove sequences that have an average base quality below this value (ignored if < 1)"/> |
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
126 <param name="qthreshold" type="integer" value="0" label="qthreshold - remove sequences that have any base quality below this value (ignored if < 1)"/> |
5
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
127 <param name="qwindowaverage" type="integer" value="0" label="qwindowaverage - remove sequences that have an average base quality below this value over a window (ignored if < 1)"/> |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
128 <param name="qwindowsize" type="integer" value="50" label="qwindowsize - number of bases in a window. Default=50."/> |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
129 <param name="rollaverage" type="integer" value="0" label="rollaverage - remove sequences that have an average base quality below this value in a rolling window (ignored if < 1)"/> |
e2e2071d2c62
Add missing qual params to trim.seqs
jj@dbw-galaxy-dev.msi.umn.edu
parents:
4
diff
changeset
|
130 <param name="qstepsize" type="integer" value="1" label="qstepsize - number of bases to move the window over. Default=1."/> |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
131 <param name="qtrim" type="boolean" truevalue="--qtrim=true" falsevalue="" checked="false" label="qtrim - trim sequences below qthreshold and put in trim output, else put in scrap "/> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
132 </when> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
133 </conditional> |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
134 <param name="flip" type="boolean" truevalue="--flip=true" falsevalue="" checked="false" label="flip - reverse complement the trimmed sequences"/> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
135 </inputs> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
136 <outputs> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
137 <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" /> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
138 <data format_source="fasta" name="trim_fasta" label="${tool.name} on ${on_string}: trim.fasta"/> |
7 | 139 <data format_source="names" name="trim_names" label="${tool.name} on ${on_string}: trim.names"> |
140 <filter>names != None</filter> | |
141 </data> | |
4 | 142 <data format_source="qfile" name="trim_qual" label="${tool.name} on ${on_string}: trim.qual"> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
143 <filter>(qual['add'] == 'yes' and len(qual['qfile'].__str__) > 0)</filter> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
144 </data> |
4 | 145 <data format_source="fasta" name="scrap_fasta" label="${tool.name} on ${on_string}: scrap.fasta"/> |
31
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
146 <data format_source="names" name="scrap_names" label="${tool.name} on ${on_string}: scrap.names"> |
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
147 <filter>names != None</filter> |
a3eed59297ea
Patches courtesy of Peter Briggs, Bioinformatics Core Facility University of Manchester
Jim Johnson <jj@umn.edu>
parents:
27
diff
changeset
|
148 </data> |
4 | 149 <data format_source="qfile" name="scrap_qual" label="${tool.name} on ${on_string}: scrap.qual"> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
150 <filter>(qual['add'] == 'yes' and len(qual['qfile'].__str__) > 0)</filter> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
151 </data> |
2
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
152 <data format="groups" name="groups_file" label="${tool.name} on ${on_string}: groups"> |
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
153 <filter>(oligo['add'] == 'yes' and len(oligo['oligos']) > 0)</filter> |
e990ac8a0f58
Migrated tool version 1.19.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
1
diff
changeset
|
154 </data> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
155 </outputs> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
156 <requirements> |
27
49058b1f8d3f
Update to mothur version 1.27 and add tool_dependencies.xml to automatically install mothur
Jim Johnson <jj@umn.edu>
parents:
24
diff
changeset
|
157 <requirement type="package" version="1.27">mothur</requirement> |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
158 </requirements> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
159 <tests> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
160 </tests> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
161 <help> |
1
fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
jjohnson
parents:
0
diff
changeset
|
162 **mothur overview** |
0
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
163 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
164 Mothur_, initiated by Dr. Patrick Schloss and his software development team |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
165 in the Department of Microbiology and Immunology at The University of Michigan, |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
166 provides bioinformatics for the microbial ecology community. |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
167 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
168 .. _Mothur: http://www.mothur.org/wiki/Main_Page |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
169 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
170 **Command Documenation** |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
171 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
172 The trim.seqs_ command provides the preprocessing features needed to screen and sort pyrosequences. The command will enable you to trim off primer sequences and barcodes, use the barcode information to generate a group file and split a fasta file into sub-files, screen sequences based on the qual file that comes from 454 sequencers, cull sequences based on sequence length and the presence of ambiguous bases and get the reverse complement of your sequences. While this analysis is clearly geared towards pyrosequencing collections, it can also be used with traditional Sanger sequences. |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
173 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
174 .. _trim.seqs: http://www.mothur.org/wiki/Trim.seqs |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
175 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
176 |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
177 </help> |
3202a38e44d9
Migrated tool version 1.15.1 from old tool shed archive to new tool shed repository
jjohnson
parents:
diff
changeset
|
178 </tool> |