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5
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1 galaxy-pcr-markers
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2 ==================
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3
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4 Scripts for design of PCR-based Marker Assays from DNA sequence variant data, and xml wrappers for use in Galaxy workflow environment
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5
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6 Also available for download at Galaxy Toolshed http://toolshed.g2.bx.psu.edu/
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7 hg clone http://toolshed.g2.bx.psu.edu/repos/john-mccallum/pcr_markers
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8
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9 NOTE that the primer design tool *design_primers.py* relies on EMBOSS ePrimer3 which depends on Primer3 1.1.4.
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10 The updated tool *design_HRM_primers.py* does not have this restriction and should work with Primer3 V2.
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11
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12 Please see development branch HRM for optimised design of high-resolution melting PCR assays using the uMelt web service at University of Utah.
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13
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14
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15 **CITATION**
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16 A Toolkit For Bulk PCR-Based Marker Design From Next-Generation Sequence Data: Application For Development Of A Framework Linkage Map In Bulb Onion (Allium cepa L.) (2012)
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17
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18 Samantha Baldwin, Roopashree Revanna, Susan Thomson, Meeghan Pither-Joyce, Kathryn Wright, Ross Crowhurst, Mark Fiers, Leshi Chen, Richard MacKnight, John A. McCallum
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19
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20 BMC Genomics 2012, 13:637 http://www.biomedcentral.com/1471-2164/13/637/abstract
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21
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22 **Acknowledgements**
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23 Current development of these tools is funded by the New Zealand Ministry for Business, Innovation & Employment project 'Virtual Institute of Statistical Genetics' (VISG)
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24 See http://www.visg.co.nz
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