annotate README_INTERPROSCAN @ 0:49e20fa2c66d

Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
author konradpaszkiewicz
date Tue, 07 Jun 2011 17:27:16 -0400
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49e20fa2c66d Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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1 #Created 07/01/2011 - Konrad Paszkiewicz, Exeter Sequencing Service, University of Exeter
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49e20fa2c66d Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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3 The attached is a crude wrapper script for Interproscan. Typically this is useful when one wants to produce an annotation which is not based on sequence
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4 similarity. E.g after a denovo transcriptome assembly, each transcript could be translated and run through this tool.
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6 Prerequisites:
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8 1. A working installation of Interproscan on your Galaxy server/cluster.
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10 Limitations:
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12 Currently it is setup to work with PFAM only due to the heavy computational demands Interproscan makes.
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14 Input formats:
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49e20fa2c66d Migrated tool version 1.0.0 from old tool shed archive to new tool shed repository
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16 The standard interproscan input is either genomic or protein sequences. In the case of genomic sequences Interproscan will of run an ORF
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17 prediction tool. However this tends to lose the ORF information (e.g. start/end co-ordinates) from the header. As such the requirement here is to input ORF
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18 sequences (e.g. from EMBOSS getorf) and to then replace any spaces in the FASTA header with underscores. This workaround generally preserves the relevant
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19 positional information.
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