Mercurial > repos > lparsons > cutadapt
changeset 27:de6cebe3c043 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 5eacc4a26d9cd55d1d34d1c35ab200733da6948f"
| author | iuc |
|---|---|
| date | Mon, 01 Nov 2021 12:14:05 +0000 |
| parents | 44e2cc14f75b |
| children | c053d4cad713 |
| files | cutadapt.xml macros.xml test-data/cutadapt_out2_cutoff.fq.gz test-data/cutadapt_rest.json |
| diffstat | 4 files changed, 599 insertions(+), 32 deletions(-) [+] |
line wrap: on
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--- a/cutadapt.xml Thu Oct 21 16:06:55 2021 +0000 +++ b/cutadapt.xml Mon Nov 01 12:14:05 2021 +0000 @@ -79,6 +79,10 @@ -j=\${GALAXY_SLOTS:-4} +#if 'json_stats' in $output_selector: + --json stats.json +#end if + #if str( $library.type ) == "single": @read1_options@ #if 'multiple_output' in $output_selector: @@ -158,6 +162,9 @@ '${read1}' #if $paired: '${read2}' + #if $library.r2.quality_cutoff2: + -Q=$library.r2.quality_cutoff2 + #end if #end if #if 'report' in $output_selector: @@ -248,7 +255,7 @@ <!-- Read Modification Options --> <section name="read_mod_options" title="Read Modification Options"> - <param argument="--quality-cutoff" type="text" value="0" label="Quality cutoff" help=" Trim low-quality bases from 5' and/or 3' ends of each read before adapter removal. Applied to both reads if data is paired. If one value is given, only the 3' end is trimmed. If two comma-separated cutoffs are given, the 5' end is trimmed with the first cutoff, the 3' end with the second."> + <param argument="--quality-cutoff" type="text" value="0" label="Quality cutoff" help=" Trim low-quality bases from 5' and/or 3' ends of each read before adapter removal. Applied to both reads for paired-end data, unless a separate value for the second read is specified. If one value is given, only the 3' end is trimmed. If two comma-separated cutoffs are given, the 5' end is trimmed with the first cutoff, the 3' end with the second."> <sanitizer> <valid initial="string.digits"><add value="," /></valid> </sanitizer> @@ -310,6 +317,7 @@ <option value="too_long_file">Too long reads: write reads that are too long (according to maximum length specified)</option> <option value="untrimmed_file">Untrimmed reads: write reads that do not contain the adapter to a separate file, instead of writing them to the regular output file (default: output to same file as trimmed)</option> <option value="multiple_output">Multiple output: create a separate file for each adapter trimmed (default: all trimmed reads are in a single file)</option> + <option value="json_stats">Statistics in JSON format</option> </param> </inputs> @@ -372,8 +380,10 @@ <filter>output_selector and 'too_long_file' in output_selector</filter> <expand macro="inherit_format_2" /> </data> - - <collection name="split_output" type="list" label="${tool.name} on ${on_string}: Split outputs" > + <data name="json_stats" format="json" from_work_dir="stats.json" label="${tool.name} on ${on_string}: Statistics (JSON)" > + <filter>output_selector and 'json_stats' in output_selector</filter> + </data> + <collection name="split_output" type="list" label="${tool.name} on ${on_string}: Split outputs" format="fastqsanger" > <discover_datasets pattern="(?P<designation>.+)\.(?P<ext>fastq.*)" directory="split" /> <filter>output_selector and 'multiple_output' in output_selector</filter> </collection> @@ -505,8 +515,8 @@ <has_text text="--discard-trimmed"/> </assert_command> </test> - <!-- Ensure rest file output works --> - <test expect_num_outputs="2"> + <!-- Ensure rest file output works, test json output --> + <test expect_num_outputs="3"> <param name="input_1" ftype="fasta" value="cutadapt_rest.fa" /> <section name="r1"> <repeat name="adapters"> @@ -518,8 +528,9 @@ </section> <param name="output_filtering" value="default"/> <param name="read_modification" value="none"/> - <param name="output_selector" value="rest_file"/> + <param name="output_selector" value="rest_file,json_stats"/> <output name="out1" file="cutadapt_rest.out" ftype="fasta"/> + <output name="json_stats" file="cutadapt_rest.json" ftype="json"/> <output name="rest_output" file="cutadapt_rest2.out" ftype="fasta"/> </test> <!-- Ensure nextseq-trim option works --> @@ -1082,6 +1093,70 @@ <has_text text="TGTAGGCCX"/> </assert_command> </test> + <!-- Ensure individual per adapter noindels parameter works --> + <test expect_num_outputs="3"> + <param name="type" value="paired_collection" /> + <param name="input_1"> + <collection type="paired"> + <element name="forward" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> + <element name="reverse" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" /> + </collection> + </param> + <section name="r1"> + <repeat name="adapters"> + <conditional name="adapter_source"> + <param name="adapter_source_list" value="user"/> + <param name="adapter" value="AGATCGGAAGAGC"/> + </conditional> + <param name="single_noindels" value=";noindels" /> + </repeat> + </section> + <section name="r2"> + <repeat name="adapters2"> + <conditional name="adapter_source2"> + <param name="adapter_source_list2" value="user"/> + <param name="adapter2" value="AGATCGGAAGAGC"/> + </conditional> + <param name="single_noindels" value=";noindels" /> + </repeat> + </section> + <output_collection name="out_pairs" type="paired"> + <element name="forward" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz" /> + <element name="reverse" decompress="True" file="cutadapt_out2.fq.gz" ftype="fastq.gz" /> + </output_collection> + </test> + <!-- Ensure specifying quality cutoff (for both reads) works --> + <test expect_num_outputs="3"> + <param name="type" value="paired_collection" /> + <param name="input_1"> + <collection type="paired"> + <element name="forward" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> + <element name="reverse" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" /> + </collection> + </param> + <param name="quality_cutoff" value="5" /> + <section name="r1"> + <repeat name="adapters"> + <conditional name="adapter_source"> + <param name="adapter_source_list" value="user"/> + <param name="adapter" value="AGATCGGAAGAGC"/> + </conditional> + </repeat> + </section> + <section name="r2"> + <repeat name="adapters2"> + <conditional name="adapter_source2"> + <param name="adapter_source_list2" value="user"/> + <param name="adapter2" value="AGATCGGAAGAGC"/> + </conditional> + </repeat> + <param name="quality_cutoff2" value="15,20"/> + </section> + <output_collection name="out_pairs" type="paired"> + <element name="forward" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz" /> + <element name="reverse" decompress="True" file="cutadapt_out2_cutoff.fq.gz" ftype="fastq.gz" /> + </output_collection> + </test> </tests> <help><![CDATA[
--- a/macros.xml Thu Oct 21 16:06:55 2021 +0000 +++ b/macros.xml Mon Nov 01 12:14:05 2021 +0000 @@ -1,6 +1,6 @@ <macros> - <token name="@TOOL_VERSION@">3.4</token> - <token name="@VERSION_SUFFIX@">2</token> + <token name="@TOOL_VERSION@">3.5</token> + <token name="@VERSION_SUFFIX@">0</token> <token name="@FASTQ_TYPES@">fastq.gz,fastq,fasta</token> <xml name="edam_ontology"> <edam_topics> @@ -31,35 +31,35 @@ #for $a in $library.r1.adapters #if $a.adapter_source.adapter_source_list == 'builtin': - -a '${a.adapter_source.adapter.fields.name}'='${a.adapter_source.adapter}${adapter_options.internal}' + -a '${a.adapter_source.adapter.fields.name}'='${a.adapter_source.adapter}${adapter_options.internal}${a.single_noindels}' #else if $a.adapter_source.adapter_source_list == 'file': - -a file:'${a.adapter_source.adapter_file}${adapter_options.internal}' + -a file:'${a.adapter_source.adapter_file}${adapter_options.internal}${a.single_noindels}' #else if str($a.adapter_source.adapter_name) != "": - -a '${a.adapter_source.adapter_name}'='${a.adapter_source.adapter}${adapter_options.internal}' + -a '${a.adapter_source.adapter_name}'='${a.adapter_source.adapter}${adapter_options.internal}${a.single_noindels}' #else - -a '${a.adapter_source.adapter}${adapter_options.internal}' + -a '${a.adapter_source.adapter}${adapter_options.internal}${a.single_noindels}' #end if #end for #for $aa in $library.r1.anywhere_adapters #if $aa.anywhere_adapter_source.anywhere_adapter_source_list == 'builtin': - -b '${aa.anywhere_adapter_source.anywhere_adapter.fields.name}'='${aa.anywhere_adapter_source.anywhere_adapter}' + -b '${aa.anywhere_adapter_source.anywhere_adapter.fields.name}'='${aa.anywhere_adapter_source.anywhere_adapter}${aa.single_noindels}' #else if $aa.anywhere_adapter_source.anywhere_adapter_source_list == 'file': - -b file:'${aa.anywhere_adapter_source.anywhere_adapter_file}' + -b file:'${aa.anywhere_adapter_source.anywhere_adapter_file}${aa.single_noindels}' #else if str($aa.anywhere_adapter_source.anywhere_adapter_name) != "": - -b '${aa.anywhere_adapter_source.anywhere_adapter_name}'='${aa.anywhere_adapter_source.anywhere_adapter}' + -b '${aa.anywhere_adapter_source.anywhere_adapter_name}'='${aa.anywhere_adapter_source.anywhere_adapter}${aa.single_noindels}' #else - -b '${aa.anywhere_adapter_source.anywhere_adapter}' + -b '${aa.anywhere_adapter_source.anywhere_adapter}${aa.single_noindels}' #end if #end for #for $fa in $library.r1.front_adapters #if $fa.front_adapter_source.front_adapter_source_list == 'builtin': - -g '${fa.front_adapter_source.front_adapter.fields.name}'='${adapter_options.internal}${fa.front_adapter_source.front_adapter}' + -g '${fa.front_adapter_source.front_adapter.fields.name}'='${adapter_options.internal}${fa.front_adapter_source.front_adapter}${fa.single_noindels}' #else if $fa.front_adapter_source.front_adapter_source_list == 'file': - -g file:'${adapter_options.internal}${fa.front_adapter_source.front_adapter_file}' + -g file:'${adapter_options.internal}${fa.front_adapter_source.front_adapter_file}${fa.single_noindels}' #else if str($fa.front_adapter_source.front_adapter_name) != "": - -g '${fa.front_adapter_source.front_adapter_name}'='${adapter_options.internal}${fa.front_adapter_source.front_adapter}' + -g '${fa.front_adapter_source.front_adapter_name}'='${adapter_options.internal}${fa.front_adapter_source.front_adapter}${fa.single_noindels}' #else - -g '${adapter_options.internal}${fa.front_adapter_source.front_adapter}' + -g '${adapter_options.internal}${fa.front_adapter_source.front_adapter}${fa.single_noindels}' #end if #end for @@ -96,35 +96,35 @@ #for $a in $library.r2.adapters2 #if $a.adapter_source2.adapter_source_list2 == 'builtin': - -A '${a.adapter_source2.adapter2.fields.name}'='${a.adapter_source2.adapter2}${adapter_options.internal}' + -A '${a.adapter_source2.adapter2.fields.name}'='${a.adapter_source2.adapter2}${adapter_options.internal}${a.single_noindels}' #else if $a.adapter_source2.adapter_source_list2 == 'file': - -A file:'${a.adapter_source2.adapter_file2}${adapter_options.internal}' + -A file:'${a.adapter_source2.adapter_file2}${adapter_options.internal}${a.single_noindels}' #else if str($a.adapter_source2.adapter_name2) != "": - -A '${a.adapter_source2.adapter_name2}'='${a.adapter_source2.adapter2}${adapter_options.internal}' + -A '${a.adapter_source2.adapter_name2}'='${a.adapter_source2.adapter2}${adapter_options.internal}${a.single_noindels}' #else - -A '${a.adapter_source2.adapter2}${adapter_options.internal}' + -A '${a.adapter_source2.adapter2}${adapter_options.internal}${a.single_noindels}' #end if #end for #for $aa in $library.r2.anywhere_adapters2 #if $aa.anywhere_adapter_source2.anywhere_adapter_source_list2 == 'builtin': - -B '${aa.anywhere_adapter_source2.anywhere_adapter2.fields.name}'='${aa.anywhere_adapter_source2.anywhere_adapter2}' + -B '${aa.anywhere_adapter_source2.anywhere_adapter2.fields.name}'='${aa.anywhere_adapter_source2.anywhere_adapter2}${aa.single_noindels}' #else if $aa.anywhere_adapter_source2.anywhere_adapter_source_list2 == 'file': - -B file:'${aa.anywhere_adapter_source2.anywhere_adapter_file2}' + -B file:'${aa.anywhere_adapter_source2.anywhere_adapter_file2}${aa.single_noindels}' #else if str($aa.anywhere_adapter_source2.anywhere_adapter_name2) != "": - -B '${aa.anywhere_adapter_source2.anywhere_adapter_name2}'='${aa.anywhere_adapter_source2.anywhere_adapter2}' + -B '${aa.anywhere_adapter_source2.anywhere_adapter_name2}'='${aa.anywhere_adapter_source2.anywhere_adapter2}${aa.single_noindels}' #else -B '${aa.anywhere_adapter_source2.anywhere_adapter2}' #end if #end for #for $fa in $library.r2.front_adapters2 #if $fa.front_adapter_source2.front_adapter_source_list2 == 'builtin': - -G '${fa.front_adapter_source2.front_adapter2.fields.name}'='${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}' + -G '${fa.front_adapter_source2.front_adapter2.fields.name}'='${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}${fa.single_noindels}' #else if $fa.front_adapter_source2.front_adapter_source_list2 == 'file': - -G file:'${adapter_options.internal}${fa.front_adapter_source2.front_adapter_file2}' + -G file:'${adapter_options.internal}${fa.front_adapter_source2.front_adapter_file2}${fa.single_noindels}' #else if str($fa.front_adapter_source2.front_adapter_name2) != "": - -G '${fa.front_adapter_source2.front_adapter_name2}'='${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}' + -G '${fa.front_adapter_source2.front_adapter_name2}'='${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}${fa.single_noindels}' #else - -G '${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}' + -G '${adapter_options.internal}${fa.front_adapter_source2.front_adapter2}${fa.single_noindels}' #end if #end for @@ -222,6 +222,7 @@ <param name="adapter_file" argument="-a" format="fasta" type="data" help="Should be of datatype FASTA" label="Choose file containing 3' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <repeat name="front_adapters" title="5' (Front) Adapters" help="Sequence of an adapter ligated to the 5' end (paired data: of the first read). The adapter and any preceding bases are trimmed. Partial matches at the 5' end are allowed. If a '^' character is prepended ('anchoring'), the adapter is only found if it is a prefix of the read. To search for a linked adapter, separate the 2 sequences with 3 dots (ADAPTER1...ADAPTER2), see Help below."> @@ -243,6 +244,7 @@ <param name="front_adapter_file" argument="-g" format="fasta" type="data" help="Should be of datatype "FASTA"" label="Choose file containing 5' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <repeat name="anywhere_adapters" title="5' or 3' (Anywhere) Adapters" help="Sequence of an adapter that may be ligated to the 5' or 3' end (paired data: of the first read). Both types of matches as described under 3' und 5' Adapters are allowed. If the first base of the read is part of the match, the behavior is as with 5' Adapters, otherwise as with 3' Adapters. This option is mostly for rescuing failed library preparations - do not use if you know which end your adapter was ligated to!"> @@ -264,6 +266,7 @@ <param name="anywhere_adapter_file" argument="-b" format="fasta" type="data" help="Should be of datatype "FASTA"" label="Choose file containing 3' or 5' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <param name="cut" argument="--cut" type="integer" value="0" optional="True" label="Cut bases from reads before adapter trimming" help="Remove bases from each read (first read only if paired). If positive, remove bases from the beginning. If negative, remove bases from the end. This is applied *before* adapter trimming." /> @@ -300,6 +303,7 @@ <param name="adapter_file2" argument="-a" format="fasta" type="data" help="Should be of datatype "FASTA"" label="Choose file containing 3' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <repeat name="front_adapters2" title="5' (Front) Adapters" help="Sequence of an adapter ligated to the 5' end of the second read in each pair. The adapter and any preceding bases are trimmed. Partial matches at the 5' end are allowed. If a '^' character is prepended ('anchoring'), the adapter is only found if it is a prefix of the read. To search for a linked adapter, separate the 2 sequences with 3 dots (ADAPTER1...ADAPTER2), see Help below."> @@ -321,6 +325,7 @@ <param name="front_adapter_file2" argument="-g" format="fasta" type="data" help="Should be of datatype "FASTA"" label="Choose file containing 5' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <repeat name="anywhere_adapters2" title="5' or 3' (Anywhere) Adapters" help="Sequence of an adapter that may be ligated to the 5' or 3' end of the second read in each pair. Both types of matches as described under under 3' und 5' Adapters are allowed. If the first base of the read is part of the match, the behavior is as with 5' Adapters, otherwise as with 3' Adapters. This option is mostly for rescuing failed library preparations - do not use if you know which end your adapter was ligated to!"> @@ -342,10 +347,16 @@ <param name="anywhere_adapter_file2" argument="-b" format="fasta" type="data" help="Should be of datatype "FASTA"" label="Choose file containing 3' or 5' adapters" /> </when> </conditional> + <param name="single_noindels" type="boolean" truevalue=";noindels" falsevalue="" checked="false" label="Disallow indels for this adapter"/> </repeat> <param name="cut2" argument="-U" type="integer" value="0" optional="True" label="Cut bases from the second read in each pair." help="Remove bases from the beginning or end of each read before trimming adapters. If positive, the bases are removed from the beginning of each read. If negative, the bases are removed from the end of each read." /> - + <param name="quality_cutoff2" type="text" optional="true" label="Optional separate quality cutoff for Read 2" help="Trim low-quality bases from 5' and/or 3' ends of each read before adapter removal. If one value is given, only the 3' end is trimmed. If two comma-separated cutoffs are given, the 5' end is trimmed with the first cutoff, the 3' end with the second. Leave blank to use the same value as for Read 1"> + <sanitizer> + <valid initial="string.digits"><add value="," /></valid> + </sanitizer> + <validator type="regex">[0-9,]+</validator> + </param> </section> </xml>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cutadapt_out2_cutoff.fq.gz Mon Nov 01 12:14:05 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/2 +TC ++ +1> +@M01368:8:000000000-A3GHV:1:1101:14518:9998/2 +CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATA ++ +CCCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF +@M01368:8:000000000-A3GHV:1:1101:18422:19051/2 +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +BCCCCFFCFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF +@M01368:8:000000000-A3GHV:1:1101:25545:21098/2 +GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT ++ +3AAA34BFFFFFGCGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-<BB?9.9BFFFB==AA/FFFFFA-@BA.;;D?F9FAB;---./99BFFFBBAE-.9B/BFB9F/9BFBB +@M01368:8:000000000-A3GHV:1:1101:5446:12248/2 +ATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGT ++ +CCDDDCCCCFFFGGGGGGGGGGHHHHHGHHHHHHHHGHHHHHHGHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHGFGHHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGGGGGGFFFFFFFFFFFFFFAFFFFFFFFFFFFFBFC?.EAFFFFFFFFAFFFFFFFFFFFFFFFEFFFF0FFFFFFF0BFFFFFFFFF?.BDCFFEDA +@M01368:8:000000000-A3GHV:1:1101:5861:6452/2 +TTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCT ++ +BCCCCFBBCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHGHHHHHHHHHHHHHGGHGGHHHHHHHGHHHHHHHHHHHHHHHHHHHGGHHHHGGGGGGEHGHHHHHHHHHHHGHHHHHHGHGHGHGGGGCGGGGGFFFFFFFFFFFFFFFFFFFFFFFF?FFEAF:DAEF9DEFFFFFFF-A.BFFFFFEFFFFEFFFFFFFF0FFEB0FFFFBFFFFFFADEFCF +@M01368:8:000000000-A3GHV:1:1102:10403:6021/2 +GGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGGGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGGGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGAGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACGCTGAAGTAGGAACCAGATGTCGGATACAGTTGACTTTAGGTAACCC ++ +AA?A34BF>AADEBGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<BAGHB?11F/FGFGGBFBGBD.11><.<<<C0<A</<<=0GFHC@-.;FF09BFFGB00B0;FE.C/:CCF0;0C0;BBBFF@.9AF.:9EFF.;.BFF/FF/;/./BFB/..;;ABF/9FF//.;AAF9BBBA;-A.B/9///9/BF////;///:9 +@M01368:8:000000000-A3GHV:1:1102:10677:23253/2 +AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGCTGTCTCTTATACA ++ +CDDDDFFFFCCFGGGGGGGGGGHHHHHGGGGHHHHHHHHHHHHHHHHGGHHGGHGGHHHHHHGHGHGHHHGGGGGGGGHHHHHFHHHFHHHHGGGGGGHHGHHGGHHHGGGCGGHFFFGGGHHGGGFHHGHHHGGGCGGHFHHGGGGGGFHGC<@GGHGHHCHE00:CFHHHFGACGFFEG:D.E;FG;EFBBFGGGFEE0CBBBFFGBFFGFGFFFFFFFF0:B0:BFFFFFFF/0.;:0F0B:BFFF +@M01368:8:000000000-A3GHV:1:1102:13809:1733/2 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+ABCCCFFFFFCFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFFFFFFBFFFFFFF/:BFFFFFFFFFFFFFFFFAFC=@-.;A +@M01368:8:000000000-A3GHV:1:1102:18842:24844/2 +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +CCCCBFFBFFFFGGGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF +@M01368:8:000000000-A3GHV:1:1102:21788:11027/2 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+BBBBBFFBBBBFGGGGGGGDGGHHHHHFHHHGGGGGHHGFHFHHFHHHGGHHHHHHHHHHHHHGHHHHHHFGHHHGGGGHHHHHHHHHGHHHGHHHHFHHHGGHGGHFFHHHHGFHHHHHHHHHHFHFHHHFHFHHHHHGGGGGHGHHHHHGHHHHHHH/FHHHHHGHGGGHHGGFBFFFFFFFFFF;DDFFFFFFF;;BA.=DDBFFFFF;-DFF.EDFFFFFFEF-AFFFFFB;FBFFFBFFFFB9B +@M01368:8:000000000-A3GHV:1:1102:5983:21093/2 +ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGT ++ +ABCCCFFFBFFCGGGGGGGGGGHHHHHHGHHGHHGGGGGHHHHHHGHHHHHGGHHHHGGGGFHHHHGFFHGHGGGFEGGHFFFE?GFFGGGHGHFGHGGGEEFHHHHGGGGGCDGGBGGGGHHHGGHGHHHDGHHHHEFHHHHGF<A.ADGG0EHFFHHHHCEFHHEGHGHHGGHHHFGGGGEF//FFFFGGGFGGGGGGG.ACDE?B?DD9F.BBBBFFFFFEF0090:BF/;:0;0009:A?...0:; +@M01368:8:000000000-A3GHV:1:1102:7765:18353/2 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+BBBBBBFFFFFFGGGGGGGGGGHHHGHHHFHGHHHHHGFHHGGHGGGHHHHHHHHHEEHGGGGGGHGHHHHGBGCHFHHHHHHGGGGGHGFHGFGHHHGGEGFGHHEGFFGHFEGG?HEBFHHF?FGGGEDG0CFGGG?E..<<-.CGGHHH0GHEGGDDC0/CA.0G/:;.;.;;;C09;BBFGBA.--./9;0C0/00;0;/./99.:9///:/9//;//;BF.D.AAD.;--@B9../;;:BF/; +@M01368:8:000000000-A3GHV:1:1103:17657:20856/2 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGAACTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHGHFHHHHHHHGFGHGEHGGHHHHHHHHHEHFHHGGGGGHFHHHHFHHHHFFHFHHHGGGGDGHHGHFGHGHGGEEFGHHBFGGGHHGGGGHHHHHFFGGGGGHGHGDGCGGCGFF@DGGGHFHH0C.GHFHFHGHHFGH0CCAECDEG0CEGGGGFFFGG;C.0;;00:BF;FFBFFF09A9A:BFFFBFF0BF0:FFFAA;.AAEFFFFEF?FFFEFF9BBE +@M01368:8:000000000-A3GHV:1:1103:23099:3794/2 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+BCCCCFFFCFFFGGGGGGGGGGHHHHGHHHHHHHHFHHHFGHF?FGGHGHHGHHHHFHHHHHFEGFHGGHDGHHHHFHHHGHFFHHGAEEGHCEHHHBGHHHBGHFGHHGGGGGFGHHFGCHHCEGE/FGHGHGGGDGGGGGDHFGHHH/E?@DDHBHH>?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF +@M01368:8:000000000-A3GHV:1:1104:13832:4398/2 +GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCT ++ +BBCCCFFFFFFFGGGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0FGHHGHHGHHHHHHHHHHFGHHGGGGGHG-:@?FAGA:.:09CB0000;9B +@M01368:8:000000000-A3GHV:1:1104:23670:4161/2 +CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTC ++ +A3AABBFFFFFFGGGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FBB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/2 +GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAA ++ +AABBBFFFFFFFGAFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/<C/CHHHHHHEDHFHGHHG0>AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B +@M01368:8:000000000-A3GHV:1:1104:4705:7727/2 +TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +ABBBAFFFFFFFGGFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9 +@M01368:8:000000000-A3GHV:1:1104:7756:4776/2 +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +BBBBBFFBFFFFGGGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A +@M01368:8:000000000-A3GHV:1:1105:11310:21761/2 +ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTCC ++ +BCCCCFCCCCCCGGGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900; +@M01368:8:000000000-A3GHV:1:1105:11578:14676/2 +GCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGT ++ +CCBCCCBBCCCCGGGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF<GGHHHHGFGHGEHGGA=A=DFFFFAFAFF?FFFFFFFFFADFAAD;FFFA:BBBAE.A.EBFFFFEF-..0BFFF9 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/2 +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTGGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTAACTATTACAG ++ +AA>>ABFDCFFFFFDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F<EEF0@C@9:AAF00;9@-9.;FBBBBB->@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F +@M01368:8:000000000-A3GHV:1:1105:15502:11453/2 +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACCTGTCTCTT ++ +CCCCCFFFCFFFGGGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFFE//090::B +@M01368:8:000000000-A3GHV:1:1105:16787:9771/2 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+CCCCCCCCCFFFGGGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9 +@M01368:8:000000000-A3GHV:1:1105:18108:12091/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACA ++ +CBBCCFCCCCCFGGGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFFFFFBFFFBFEFFFF +@M01368:8:000000000-A3GHV:1:1105:18331:16683/2 +TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATATCT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;0B +@M01368:8:000000000-A3GHV:1:1105:20577:18421/2 +ATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATT ++ +?ABBBFFFFFFF4CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@<AFH1CDDGCGG..<C--:D000CF0::C009B9F/B..909C9....009.;;/900:A;;A////:BFFF/BFBFFFF.9.//;999//;9 +@M01368:8:000000000-A3GHV:1:1105:21099:2895/2 +TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATCGACCTGTGATCCATCGTG ++ +DDCDCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHFHFHHHHHHHHDEGGGHHIIHHIHEGDFHFC1EAFHFFHEGHHGHFHHGHFHFFFFGGGGHGHFFGFHEGHHGHHHHHGGGGGHECFGADHFHGGCGGEGHG/EGFHHGGDGHFHFFCECC@E?F1FH?ECGGGGHFCAEAFFHBC0CACF0BFFGGGGGFB:BEFEDGD0::;9FBB0:BF9@=...;9-AB;B0;F00:;.9: +@M01368:8:000000000-A3GHV:1:1105:26469:19245/2 +GGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTTGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCT ++ +AAABBBBFFFFFGGGGGGGGGGHHHGGGGGHHFFGHHHHFHHHFFHFHHHFHHHHHFGFHHHHHGHHHHGGGGHHHGHHFHHHHHHHGHHHHHHHGGHGGHHHHHHHHHHHGHHHHHHHHHHGHHHHGHGHHGGCGGGHHHH23FFHHHHGHGCGHHHHGGHFHGHBCCFFFFFFF/ECCDFDFEFFFFBDAFF?DADE??BFFFF=A-.AAAA.BFFFFED..BF/BFFEFFFFBFFFFFFFFFFBFB:: +@M01368:8:000000000-A3GHV:1:1105:7169:17145/2 +CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGGTAATAGGGGGATAGACCTGTGATC ++ +BBBBBBFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHFHHHHHHHGGGGHHHHHHHHBGHHHHGFDGFHGGHGGHHHHHHHHHGHHHHGGGGGHGFHHHEHHHGFGHHHHGGGGGGHEHH3FGHHHGGGGGGHHECEGDFGFCGDHHHHFGGGC?@?CDGHGGGGC-:.ECDGDGGGGGF0.00BFGGGF/BD.00FG..;9.9;E.;BFFFFFF.9-.99///9BB///; +@M01368:8:000000000-A3GHV:1:1106:15481:16176/2 +AAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTA ++ +BBBBAFFFFFFFGGGGGGGFGGHHHHHHHHFHHHFHHHHGHHHHHHHHGHHHGHHEGHHHGDGCHHHHHHHHHGEHEHHHHHHHFHGGEGGHHHHHHHGEGHGHHDHHHHHHHHEHFHGHGHHHGEGCEHHFHHHGHHHHHHHF?GHHHFEFFAFFHHGG?;@DDFFB?@B;BDAFFFEBBD>D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB +@M01368:8:000000000-A3GHV:1:1106:17389:18671/2 +GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACCC ++ +BBCCCFFFCBCCGGGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA<DBGFGHBG/..DGFCDGD.:0:.;CFGHEHBF.A-99CFGGFF0C0C0BF0;CA9/B:///;//BF/9B9?-..:;.;9.9A././;B//F.B?A////9;: +@M01368:8:000000000-A3GHV:1:1106:22927:3863/2 +AAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAGCG ++ +AAAAAFFFFFFFGGGGGGFGGGHHGGHHHGGGGGGHGHGDFGHFGGGGFHHHHHGGGGGGGGFHGDFGGGGHGG?@EFHGEFEHEGFHHHHHF2FEHHGHGDCEAD?GFDFGGHBGHHFEHHHHHHHHHDFGHGHHGBDFG0FFHFGGFHHH=GGBF-AACEHEHHGGGHHHGHHFHCCFEBB0FFFFFF/;0;;FFFBFE.AA0BF0??-AD/////;:BFFBFFBBBFFFFFB/.9ADDAD-D;-;=A +@M01368:8:000000000-A3GHV:1:1106:23625:4236/2 +CACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGGTGGGCTATTTAGGTTT ++ +BBBBBFFFFFFFEGGGGGGGGGGHHHHHHHHGHGGHHHHGFGGG?HGGHHHHHHHGGHGFGHGEEGFHHHHHHHHHHHFHHFHHGGCGGGHHHHBFGFFHGGGEFGHHGFFEEFHGGDGEHFHHHGGGCCCGHHG?EGFGGGFED@D?CEDFHEHGHFFHFHHGCGG.:CGGD:AEDCG?C?BFFFGGG/CEFBFEFFF/;:BBB;CFGD.:/:B:BB/BFFFBBFBFFAA9A..9-9@:/./;B/9/:/; +@M01368:8:000000000-A3GHV:1:1106:26031:14826/2 +CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGACCTAGTGGTGATATAGGGGTGTCCCGCGTT ++ +BBBBBFFFFFDFGGGGGGGFGGGGHGGHHHHHHHGFFHHCHGGGGGHHHHHHFHFFGFGHHHHHG0EEEHHGHFFGFFHGGGCFGEFHGGGGGHGFEEGEFHGHFFGFGGGGFGDGGGG?DCCCCCDDFGHHFHCGHGHHGHHFBAFGGHE.<DCDD<CD;GFFEFHH.@?A//B0F0/;09CFFBBCF0;;F;B0;009FBFFB9.9ADDFFFFAACAC;-9;BB/B././9//;//...-;///---;9 +@M01368:8:000000000-A3GHV:1:1106:27607:15990/2 +CCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAG ++ +BCCCCFFFFFFFGGGGGGGGGGHGGHHHGHHHHGHHFHHHHHHGHHHHHHHHGGHHHHGGGGFHHHHHHGHHHHHHHHFGHHHHHCHGHGHHGGGGGGGHGFFGG@CEDGHHFHHCFGBDDGGG?AFGFGGGGAEAFDFFFFFFFECFAFFFFFFFFFFFFFFFFFFFBFFEFFFFFFFFFFDAD;=DADFF-.A.BB 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+?ABBBFFFFFFFGGGGGGGGGGGGGHHHHHGGHHHHHHHHHHHGHHHHHHHGGHHHHGHHFHHHGGGGHHHHHHHHHHGHHHHHGHHHFHGGHGGFHHHHHFGFGFHHHHHHHHHHHHFHHHFFHHHHGGGGGGGFFHHGGHHHHHHHFHHHHHGGFDHGHGGGGBFFFFFBAD@>;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F +@M01368:8:000000000-A3GHV:1:1107:18714:24788/2 +GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCT ++ +BCCCCCFFFFFFGGGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.;A +@M01368:8:000000000-A3GHV:1:1107:21355:8333/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTAC ++ +AABCCFCCCCCFGGGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BFF +@M01368:8:000000000-A3GHV:1:1107:22864:15712/2 +GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGCCGAGGG ++ +AABCCFFFFFFFGGGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBBBB9CFFB0C00;000;:CFFB;:...D?>:@=:9-@ +@M01368:8:000000000-A3GHV:1:1107:24817:14233/2 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTT ++ +CCCCBFFFFFFFGGGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF<<//ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B +@M01368:8:000000000-A3GHV:1:1107:24830:14440/2 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+BBAAAFFFFFFFGGGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBFF +@M01368:8:000000000-A3GHV:1:1108:25084:18127/2 +CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG ++ +CCCCCFFFFFFFGGGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B; +@M01368:8:000000000-A3GHV:1:1108:7626:10048/2 +AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTA ++ +BCCDDFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFFDFFFFFB-<>;>@BE<: +@M01368:8:000000000-A3GHV:1:1109:11270:12255/2 +CTACCTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGG ++ +BCCCCFFFFFFFGGGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B<FFFBFFFFFFFFFFFF0FBFFFFFFFFFFFFFFFBDDDFEFDFF@--@-9.<:000. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/2 +CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTCTGTCTCTTATACACA ++ +CCCCCFFFFFFFGGGGFGGGGGHGGHGGGHHHHHHHHHHHHHGGGGGHFHHHHHHFHHHHHFHHHGGGGGHEFHCEEHHHGGGGFGGGFGGGGHEGGGEHFHHHHDGGF?AGFHGGGGGGGGHGGGGGGGHHHHHHGFFFHHHHFCEFHFHGF<EGFHFGGAGBFHHHG/C.;C:0:GGG00CFGEFGBA.9C:BBFFGFGGFFFBFGAGG.D..:A.DFFEFFFBFFF0099:9BB0BFB +@M01368:8:000000000-A3GHV:1:1109:21978:9490/2 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+CCCDCCCCCDDFGGGGGGGGGGGEGGHHHHHHGHHHHGHHHHHHGGGFGHGFHHHHHHHHHHHHHHHFHGHHHHHGGHHHHHHBGHHGGCGGHEHHHFHHHHFHHFHHHHHHGHGGHGGHHHHHHHHHGHHFFHHHHHGHHHHHHHGHHGHHGG?FGHHGHHHHHHHHHHHGGHHHHHH?CEHGFHGGF--@DFFFFFAA;DDFFFFFF?@FC.A=DD-.AFFFF;->;.ED.FFFFFFF-.:00009BBF +@M01368:8:000000000-A3GHV:1:1109:24217:19077/2 +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTCTGT ++ +BBBBBFFFFFFFGFEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2D<FFFFAFDFF<FF1FGGBHHFF0>AGHGG00<G<<DGBGDD0<.;CGG;;:;CFFHHHHHHG?D.;.;/ACD.;FFFB0CFBFGGFGGB0;EFAFF9B/:BB/;;AEFF//:AF-A.;//:B///;BFBB:; +@M01368:8:000000000-A3GHV:1:1109:25237:11722/2 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+>>AAAFFFFFFG#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFFFFE/:9 +@M01368:8:000000000-A3GHV:1:1109:8763:7859/2 +GGTTCCTACTTCAGGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG ++ +>AAABFFFFFFFGGGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB +@M01368:8:000000000-A3GHV:1:1110:12808:18367/2 +GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCT ++ +ABCCCCDDDFFFGGGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:;F +@M01368:8:000000000-A3GHV:1:1110:14751:26540/2 +ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACCCT ++ +BBBBBFBBBBBFGGGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:FB +@M01368:8:000000000-A3GHV:1:1110:15519:10008/2 +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACA ++ +BBCBCFFFFFFFGGGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF +@M01368:8:000000000-A3GHV:1:1110:16559:7507/2 +GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT ++ +>AABBCCCCFFFGGGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9: +@M01368:8:000000000-A3GHV:1:1110:16700:6843/2 +GTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTCTGTCTCTTATACACATTTGTCGCT ++ +CBCCDFFFFFFFGGGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF0B0:000<00<BFBB0<00000<99 +@M01368:8:000000000-A3GHV:1:1110:18014:7856/2 +CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAGCTCGGGGGTCGCCGTGTATTTTTACAAAAAA ++ +CDDDBFCDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFB?DFF---;--D;9AFB0B:B0::00;0.;----.--.-9-:/00::090:0;..: +@M01368:8:000000000-A3GHV:1:1110:23303:11846/2 +GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTA ++ +BBCBBFFFFFCFGGGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB +@M01368:8:000000000-A3GHV:1:1111:13195:14562/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACG ++ +ABBBBFBBBBBFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFFFFFFF/BFF/BFFFEBFFFEFFFFFFFFF@B@ +@M01368:8:000000000-A3GHV:1:1111:14287:20817/2 +ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATCC ++ +CCCCBFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGC<FGHH?EGGG@DH.@ECEGGGGGGFGFFB0FGGGGGGEGGGFGEFEF0BE0AFFBFFFF9.-.00;000;;90;99 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/2 +CGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACCCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCA ++ +BBBBABFFFFFFGGGGGGGGGGHHCGHHHHHHHGHHHHHHHHHHHGDGEHHHHHHGHHHHHHHHHHHHHHHGGEFGHHHHHHHHBGFHHHHHHHHHHHHHHGHGGGGCEFGGCGHHHHFHHHHGHHHHFGHHHHHHGHGHGHHBCA?B-9FGFG?D;BD?9BFFFFFFF;DFFFF-.A;ADF-@=EF??A.BFFFEF>FDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF +@M01368:8:000000000-A3GHV:1:1111:7437:24508/2 +ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTCTGTCTCTTATACACATCTGACGCTGCCG ++ +BBBBBFFBABBBGGGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFFFFFFFEFBFFFB/FFFFFFF//9A..;F- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/2 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-<CCBGHHHGEFFFFHH.C/<AEGHHEG.EEA0GE0:FB0CFGF:A?FFBF??FG0B;FGGGGFGE?BBF;BFBFFFF/F/B?DDF.;.;:ADD.BA/BBFFB:BFE +@M01368:8:000000000-A3GHV:1:1111:9655:13916/2 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATTGCGTGGACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +ABBB>FFFFFFFGGGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF: +@M01368:8:000000000-A3GHV:1:1112:10272:7828/2 +GTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAG ++ +BBCCBFFCCCCCGGGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGGGGGFBBFGFBFBBBGGGB0000;CD?DFFAFF9= +@M01368:8:000000000-A3GHV:1:1112:9832:16531/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTAC ++ +BBBBBFBBBBBFGGGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/; +@M01368:8:000000000-A3GHV:1:1112:9832:6701/2 +GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT ++ +BBBBAFFFFFFFGGGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF +@M01368:8:000000000-A3GHV:1:1113:13532:8618/2 +GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT ++ +CDDDBFFFFFFFGGGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09:: +@M01368:8:000000000-A3GHV:1:1113:21679:18011/2 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCTGTCTCTTATACACATCTGA ++ +BCCCBFFFFFFFGGGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC-><FGFGEHEBDF0DGFFFFGGFFHCE.;.A;CF0;BFBFFGF/9C0;0CFGGF0F09FGGGFFFBBFEFFFB0FFEF0; +@M01368:8:000000000-A3GHV:1:1113:25528:14016/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTAC ++ +BAABCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGHGHHHHHHHHHGGGGHHHHHHHHHFHHHHHHHHHHHHGGGGGHHHHHHHGHHHHHHHHHHGGHHHHGHHGGHHHEGGGGGHHHHHHHHHGHHHHHGHHHHHGGHGHGHHGGGGGGGGGFEACFFFFFFFFFFFFFFFDFFFAFFFFBFAB@EFAAEFFFFF.ACF.BBFFEBFFFEB;FFFFFFFA/BFBFBBBFFFBFFFFFED.>DFFF +@M01368:8:000000000-A3GHV:1:1113:5741:16959/2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTAC ++ +BBBBCFCCCCCFGGGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/BF +@M01368:8:000000000-A3GHV:1:1114:10130:11959/2 +ATCAGAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGT ++ +BCCDDFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAEEDG.CGCGHC0CGBFHGFBBF0ABDDEFF@?--:BB@.;:BF; +@M01368:8:000000000-A3GHV:1:1114:14540:5315/2 +CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCC ++ +CCCCCFFCCDCCGGGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:BA +@M01368:8:000000000-A3GHV:1:1114:15066:16302/2 +TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAG ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0; +@M01368:8:000000000-A3GHV:1:1114:16639:15258/2 +TTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT ++ +CDCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF +@M01368:8:000000000-A3GHV:1:1114:2404:13066/2 +ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACTGTCTCTTATACACAT ++ +CCCCCFFFFFFCGGGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFFFF.9//;FFFFF/BFFB
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/cutadapt_rest.json Mon Nov 01 12:14:05 2021 +0000 @@ -0,0 +1,85 @@ +{ + "tag": "Cutadapt report", + "schema_version": [0, 1], + "cutadapt_version": "3.5", + "python_version": "3.9.7", + "command_line_arguments": [ + "-j=1", + "--json", + "stats.json", + "-a", + "AAAGATG", + "--rest-file=rest_output.fa", + "--output=out1.fa", + "--error-rate=0.1", + "--times=1", + "--overlap=3", + "--action=trim", + "cutadapt_rest_fa.fa" + ], + "cores": 1, + "input": { + "path1": "cutadapt_rest_fa.fa", + "path2": null, + "paired": false + }, + "read_counts": { + "input": 5, + "filtered": { + "too_short": null, + "too_long": null, + "too_many_n": null, + "too_many_expected_errors": null, + "casava_filtered": null, + "discard_trimmed": null, + "discard_untrimmed": null + }, + "output": 5, + "reverse_complemented": null, + "read1_with_adapter": 5, + "read2_with_adapter": null + }, + "basepair_counts": { + "input": 97, + "input_read1": 97, + "input_read2": null, + "quality_trimmed": null, + "quality_trimmed_read1": null, + "quality_trimmed_read2": null, + "output": 35, + "output_read1": 35, + "output_read2": null + }, + "adapters_read1": [ + { + "name": "1", + "total_matches": 5, + "on_reverse_complement": null, + "linked": false, + "five_prime_end": null, + "three_prime_end": { + "type": "regular_three_prime", + "sequence": "AAAGATG", + "error_rate": 0.1, + "indels": true, + "error_lengths": [7], + "matches": 5, + "adjacent_bases": { + "A": 0, + "C": 0, + "G": 5, + "T": 0, + "": 0 + }, + "dominant_adjacent_base": null, + "trimmed_lengths": [ + {"len": 7, "expect": 0.0, "counts": [1]}, + {"len": 12, "expect": 0.0, "counts": [1]}, + {"len": 14, "expect": 0.0, "counts": [2]}, + {"len": 15, "expect": 0.0, "counts": [1]} + ] + } + } + ], + "adapters_read2": null +}