Mercurial > repos > marpiech > rnaseq_pro_workflow_tools
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author | marpiech |
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date | Wed, 01 Feb 2017 10:29:17 -0500 |
parents | db5d126bf8d0 |
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<tool id="plotheatmap" name="plotHeatmap" version="1.0"> <description>Plot heatmap</description> <requirements> <requirement type="package" version="3.0.1">r-gplots</requirement> </requirements> <stdio> <exit_code range="1:" /> </stdio> <command> <![CDATA[ $__tool_directory__/tools/script.R $main.count_matrix $main.stats_matrix $filter.log_fc $filter.log_cpm $filter.pvalue $filter.fdr $heatmap.cluster_row $heatmap.cluster_col $heatmap.hclust_method $plotparm.col_marg $plotparm.row_marg $plotparm.pdf_width $plotparm.pdf_height > $rscript_log 2> $rerror_log ]]> </command> <inputs> <section name="main" title="Main parameters" expanded="True"> <param name="count_matrix" type="data" format="data" label="Output dataset 'count_matrix' from tool countMatrixForEdger"/> <param name="stats_matrix" type="data" format="data" label="Output dataset 'output' from tool edgeR"/> </section> <section name="filter" title="Filtering parameters" expanded="True"> <param name="log_fc" type="float" value="0.5" label="A log FC threshold"/> <param name="log_cpm" type="float" value="0.5" label="A log CPM threshold"/> <param name="pvalue" type="float" value="0.5" label="A PValue threshold"> <validator type="in_range" min="0" max="1" /> </param> <param name="fdr" type="float" value="0.5" label="A FDR threshold"> <validator type="in_range" min="0" max="1" /> </param> </section> <section name="heatmap" title="Heatmap parameters"> <param name="cluster_row" type="select" label="Determines if the row dendrogram should be reordered"> <option value="No" >No </option> <option value="Yes" selected="true" >Yes </option> </param> <param name="cluster_col" type="select" label="Determines if the column dendrogram should be reordered"> <option value="No" selected="true">No </option> <option value="Yes" >Yes </option> </param> <param name="hclust_method" type="select" label="Hierarchical clustering method"> <option value="ward.D" >ward.D </option> <option value="ward.D2" >ward.D2 </option> <option value="single" >single </option> <option value="complete" >complete </option> <option value="average" selected="true">average </option> <option value="mcquitty" >mcquitty </option> <option value="median" >median </option> <option value="centroid" >centroid </option> </param> </section> <section name="plotparm" title="Plot parameters"> <param name="col_marg" type="float" value="7" label="Margins for column names"/> <param name="row_marg" type="float" value="8" label="Margins for row names"/> <param name="pdf_width" type="float" value="7" label="Width of the graphics region in inches (pdf width)" /> <param name="pdf_height" type="float" value="7" label="Height of the graphics region in inches (pdf height)" /> </section> </inputs> <outputs> <data format="pdf" name="haetamappdf" from_work_dir="heatmap.pdf" > <filter>(outftype == 'pdf')</filter> </data> <data name="rscript_log" format="data" /> <data name="rerror_log" format="data" /> </outputs> <tests> <test> <param name="count_matrix" value="count.matrix"/> <param name="stats_matrix" value="stats.data"/> <param name="log_fc" value="1"/> <param name="log_cpm" value="2"/> <param name="pvalue" value="0.05"/> <param name="fdr" value="0.1"/> <output name="haetamappdf" file="heatmap.pdf" ftype="pdf" compare="sim_size" /> <output name="rscript_log" file="rscript.log"/> <output name="rerror_log" file="rerror.log"/> </test> </tests> <help> <![CDATA[ **What it does** It takes a list of genes basen on set filtering criteria and draws them on heatmap. **Example** The following filtering options: A log FC threshold = 0.5 A log CPM threshold = 0.5 A PValue threshold = 0.05 A FDR threshold = 0.1 mean that you select genes whose values in output table from edgeR are: * absolute value of logFC is greater than or equal 0.5 * logCPM is greater than or equal 0.5 * PValue is less than or equal to 0.05 * FDR is less than or equal to 0.1 ]]> </help> <citations> </citations> </tool>