Mercurial > repos > md-anderson-bioinformatics > heat_map_creation
view heatmap.sh @ 42:2f57ac512f46 draft
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author | insilico-bob |
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date | Mon, 06 Jul 2020 14:23:38 -0400 |
parents | 8acca16f3921 |
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#echo "1: " $1" 2: " $2" 3: " $3" 4: "$4" 5: "$5 " 6: "$6 "7: "$7" 8: "$8 " 9: "$9" 10: "${10}" 11: "${11} "12: "${12} #echo " 13: "${13}" 14: "${14}" 15: "${15}" 16: "${16} "17: "${17}" 18: "${18}" 19: "${19}" 20: "${20}" 21: "${21} " 22: "${22}" 23:" ${23} #Count total number of parameters and classification parameters parmSize=0 classSize=0 matrixSize=0 for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) parmSize=$((parmSize+1)) if [ $currParm = "classification" ] then classSize=$((classSize+1)) fi done #Get tool data and tool install directories tooldir=$1 tooldata=$2 #create temp directory for row and col order and dendro files. tdir=$tooldata/$(date +%y%m%d%M%S) mkdir $tdir #echo "tdir: "$tdir #Extract parameters for row and column order and dendro files rowOrderFile=$tdir/ROfile.txt rowDendroFile=$tdir/RDfile.txt colOrderFile=$tdir/COfile.txt colDendroFile=$tdir/CDfile.txt rowOrderJson='"order_file": "'$rowOrderFile'",' rowDendroJson='"dendro_file": "'$rowDendroFile'",' colOrderJson='"order_file": "'$colOrderFile'",' colDendroJson='"dendro_file": "'$colDendroFile'",' #BEGIN: Construct JSON for all non-repeating parameters parmJson='{' rowConfigJson='"row_configuration": {' colConfigJson='"col_configuration": {' ctr=0 for i in "$@"; do if [ $ctr -gt 1 ] then currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm != "matrix_files" ] && [ $currParm != "row_configuration" ] && [ $currParm != "col_configuration" ] && [ $currParm != "classification" ] && [ $currParm != "chm_name" ] then #Parse pipe-delimited parameter parameter parmJson=$parmJson' "'$(cut -d'|' -f1 <<< $i)'":"'$(cut -d'|' -f2 <<< $i)'",' fi if [ $currParm = "chm_name" ] then currVal=$(cut -d'|' -f2 <<< $i) currEdit=$(echo "$currVal" | sed 's/\//_/g') parmJson=$parmJson' "'$(cut -d'|' -f1 <<< $i)'":"'$currEdit'",' fi if [ $currParm = "row_configuration" ] then rowOrder=$(cut -d'|' -f3 <<< $i) rowDistance=$(cut -d'|' -f5 <<< $i) rowAgglomeration=$(cut -d'|' -f7 <<< $i) rowCuts=$(cut -d'|' -f9 <<< $i) rowLabels=$(cut -d'|' -f11 <<< $i) dataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$rowLabels'"]' if [ $rowOrder = 'Hierarchical' ] then rowConfigJson=$rowConfigJson$rowOrderJson$rowDendroJson fi rowConfigJson=$rowConfigJson'"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'",'$dataTypeJson'},' fi if [ $currParm = "col_configuration" ] then colOrder=$(cut -d'|' -f3 <<< $i) colDistance=$(cut -d'|' -f5 <<< $i) colAgglomeration=$(cut -d'|' -f7 <<< $i) colCuts=$(cut -d'|' -f9 <<< $i) colLabels=$(cut -d'|' -f11 <<< $i) dataTypeJson='"'$(cut -d'|' -f10 <<< $i)'":["'$colLabels'"]' if [ $colOrder = 'Hierarchical' ] then colConfigJson=$colConfigJson$colOrderJson$colDendroJson fi colConfigJson=$colConfigJson'"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'",'$dataTypeJson'},' fi fi ctr=$((ctr+1)) done #END: Construct JSON for all non-repeating parameters #echo "rowCuts: "$rowCuts #echo "colCuts: "$colCuts #echo "ROW CONFIG JSON: "$rowConfigJson #echo "COL CONFIG JSON: "$colConfigJson #BEGIN: Construct JSON for data layers matrixJson='"matrix_files": [ ' inputMatrix='' for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm = "matrix_files" ] then #Parse pipe-delimited parameter parameter matrixJson=$matrixJson' {"'$(cut -d'|' -f2 <<< $i)'":"'$(cut -d'|' -f3 <<< $i)'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'","'$(cut -d'|' -f6 <<< $i)'":"'$(cut -d'|' -f7 <<< $i)'"}' inputMatrix=$(cut -d'|' -f3 <<< $i) fi done matrixJson=$matrixJson"]," #END: Construct JSON for data layers #BEGIN: Construct JSON for classification files classJson='"classification_files": [ ' classIter=0 for i in "$@"; do currParm=$(cut -d'|' -f1 <<< $i) if [ $currParm = "classification" ] then classIter=$((classIter+1)) #Parse pipe-delimited 3-part classification bar parameter className=$(cut -d'|' -f3 <<< $i) if [[ -z "$className" ]]; then className="covar"$classIter fi classJson=$classJson' {"'$(cut -d'|' -f2 <<< $i)'":"'$className'","'$(cut -d'|' -f4 <<< $i)'":"'$(cut -d'|' -f5 <<< $i)'"' classCat=$(cut -d'|' -f7 <<< $i) classColorType=$(cut -d'_' -f2 <<< $classCat) classJson=$classJson',' classJson=$classJson' "position":"'$(cut -d'_' -f1 <<< $classCat)'","color_map": {"type":"'$classColorType'"}}' if [ $classIter -lt $classSize ] then classJson=$classJson',' fi fi done classJson=$classJson']' #END: Construct JSON for classification files #Add build platform JSON for distinction between GenePattern and Galaxy Builders platformJson='"build_platform": "Galaxy",' parmJson=$parmJson$matrixJson$rowConfigJson$colConfigJson$platformJson$classJson parmJson=$parmJson'}' #echo "HEATMAP PARAMETERS JSON: "$parmJson #run R to cluster matrix output="$(R --slave --vanilla --file=$tooldir/CHM.R --args $inputMatrix $rowOrder $rowDistance $rowAgglomeration $colOrder $colDistance $colAgglomeration $rowOrderFile $colOrderFile $rowDendroFile $colDendroFile $rowCuts $colCuts $rowLabels $colLabels 2>&1)" rc=$?; if [ $rc != 0 ] then echo $output; if [ `echo "$output" | grep -c "Inf in foreign function call"` -gt 0 ] then echo ""; echo "R CLUSTERING: Error in clustering the matrix provided (View Details - stdout). " echo "Note: This error can occur when:" echo " 1. There is invalid numeric data in the matrix provided. Try using Matrix Manipulation tools to fix invalid data."; echo " 2. There is no variation in a row or column in the matrix. Try a different distance measure or remove rows/columns without variation using Matrix Manipulation tools."; echo " 3. A covariate file has inadvertently been selected as an Input Matrix. Check your Input Matrix entry."; fi exit $rc; fi #call java program to generate NGCHM viewer files. java -jar $tooldir/GalaxyMapGen.jar "$parmJson" #clean up tempdir rm -rf $tdir