changeset 10:1b0182d3d262 draft

Uploaded
author insilico-bob
date Thu, 26 Jan 2017 10:15:31 -0500
parents c71752dc05d6
children 80ab17e6cc42
files ._.DS_Store ._CHM.R ._mda_heatmap_gen.xml ._ngchm-matrix-functional-test-data CHM.R GalaxyMapGen.jar heatmap.sh mda_heatmap_gen.xml mda_heatmap_viz.zip ngchm-matrix-functional-test-data/._.DS_Store ngchm-matrix-functional-test-data/._400x400.txt ngchm-matrix-functional-test-data/400x400-column-covariate.txt ngchm-matrix-functional-test-data/400x400-row-covariate.txt ngchm-matrix-functional-test-data/400x400.txt ngchm-matrix-functional-test-data/Galaxy400x400-noCovariates.ngchm
diffstat 15 files changed, 800 insertions(+), 0 deletions(-) [+]
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Binary file ._.DS_Store has changed
Binary file ._CHM.R has changed
Binary file ._mda_heatmap_gen.xml has changed
Binary file ._ngchm-matrix-functional-test-data has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/CHM.R	Thu Jan 26 10:15:31 2017 -0500
@@ -0,0 +1,146 @@
+### This method generates a row and column ordering given an input matrix and ordering methods.
+###
+### matrixData - numeric matrix 
+### rowOrderMethod - Hierarchical, Original, Random
+### rowDistanceMeasure - For clustering, distance measure. May be: euclidean, binary, manhattan, maximum, canberra, minkowski, or correlation.
+### rowAgglomerationMethod - For clustering, agglomeration method.  May be:  'average' for Average Linkage, 'complete' for Complete Linkage,
+###                                                                          'single' for Single Linkage, 'ward', 'mcquitty', 'median', or 'centroid'.
+### colOrderMethod 
+### colDistanceMeasure
+### colAgglomerationMethod
+### rowOrderFile - output file of order of rows 
+### rowDendroFile - output file of row dendrogram  
+### colOrderFile - output file of order of cols
+### colDendroFile - output file of col dendrogram
+### rowCut - For rows the number of classifications to automatically generate based on dendrogram into a classification file.  0 for turned off.
+### colCut - For columns the number of classifications to automatically generate based on dendrogram into a classification file.  0 for turned off.
+
+performDataOrdering<-function(dataFile, rowOrderMethod, rowDistanceMeasure, rowAgglomerationMethod, colOrderMethod, colDistanceMeasure, colAgglomerationMethod,rowOrderFile, colOrderFile, rowDendroFile, colDendroFile, rowCut, colCut)
+{ 
+   dataMatrix = read.table(dataFile, header=TRUE, sep = "\t", row.names = 1, as.is=TRUE, na.strings=c("NA","N/A","-","?"))
+   rowOrder <-  createOrdering(dataMatrix, rowOrderMethod, "row", rowDistanceMeasure, rowAgglomerationMethod)  
+   if (rowOrderMethod == "Hierarchical") {
+      writeHCDataTSVs(rowOrder, rowDendroFile, rowOrderFile)
+      writeHCCut(rowOrder, rowCut, paste(rowOrderFile,".cut", sep=""))
+   } else {
+      writeOrderTSV(rowOrder, rownames(dataMatrix), rowOrderFile)
+   }
+
+   colOrder <-  createOrdering(dataMatrix, colOrderMethod, "col", colDistanceMeasure, colAgglomerationMethod)  
+   if (colOrderMethod == "Hierarchical") {
+      writeHCDataTSVs(colOrder, colDendroFile, colOrderFile)
+      writeHCCut(colOrder, colCut, paste(colOrderFile,".cut", sep=""))
+   } else {
+      writeOrderTSV(colOrder, colnames(dataMatrix), colOrderFile)
+   }
+}
+
+#creates output files for hclust ordering
+writeHCDataTSVs<-function(uDend, outputHCDataFileName, outputHCOrderFileName)
+{
+   data<-cbind(uDend$merge, uDend$height, deparse.level=0)
+   colnames(data)<-c("A", "B", "Height")
+   write.table(data, file = outputHCDataFileName, append = FALSE, quote = FALSE, sep = "\t", row.names=FALSE)
+ 
+   data=matrix(,length(uDend$labels),2);
+   for (i in 1:length(uDend$labels)) {
+      data[i,1] = uDend$labels[i];
+      data[i,2] = which(uDend$order==i);
+   }
+   colnames(data)<-c("Id", "Order")
+   write.table(data, file = outputHCOrderFileName, append = FALSE, quote = FALSE, sep = "\t", row.names=FALSE)
+}
+
+#creates order file for non-clustering methods
+writeOrderTSV<-function(newOrder, originalOrder, outputHCOrderFileName)
+{
+   data=matrix(,length(originalOrder),2);
+   for (i in 1:length(originalOrder)) {
+      data[i,1] = originalOrder[i];
+      data[i,2] = which(newOrder==originalOrder[i]);
+   }
+   colnames(data)<-c("Id", "Order")
+   write.table(data, file = outputHCOrderFileName, append = FALSE, quote = FALSE, sep = "\t", row.names=FALSE)
+}
+
+#creates a classification file based on user specified cut of dendrogram
+writeHCCut<-function(uDend, cutNum, outputCutFileName)
+{
+   if (cutNum < 2) {
+      return()
+   }
+   print (paste("Writing cut file ", outputCutFileName))
+   cut <- cutree(uDend, cutNum);
+   id <- names(cut);
+   data=matrix(,length(cut),2);
+   for (i in 1:length(cut)) {
+      data[i,1] = id[i];
+      data[i,2] = sprintf("Cluster %d", cut[i]);
+   }
+
+   write.table(data, file = outputCutFileName, append = FALSE, quote = FALSE, sep = "\t", row.names=FALSE, col.names = FALSE);
+}
+
+
+createOrdering<-function(matrixData, orderMethod, direction, distanceMeasure, agglomerationMethod)
+{
+  ordering <- NULL
+
+  if (orderMethod == "Hierarchical")
+  {
+
+    # Compute dendrogram for "Distance Metric"
+    distVals <- NULL
+    if(direction=="row") {
+      if (distanceMeasure == "correlation") {
+        geneGeneCor <- cor(t(matrixData), use="pairwise")
+        distVals <- as.dist((1-geneGeneCor)/2)
+      } else {
+        distVals <- dist(matrixData, method=distanceMeasure)
+      }
+    } else { #column
+      if (distanceMeasure == "correlation") {
+        geneGeneCor <- cor(matrixData, use="pairwise")
+        distVals <- as.dist((1-geneGeneCor)/2)
+      } else {
+        distVals <- dist(t(matrixData), method=distanceMeasure)
+      }
+    }
+
+#    if (agglomerationMethod == "ward") {
+#      ordering <- hclust(distVals * distVals, method="ward.D2")
+#    } else {
+      ordering <- hclust(distVals, method=agglomerationMethod)
+#    }
+  }
+  else if (orderMethod == "Random")
+  {
+    if(direction=="row") {
+       headerList <- rownames(matrixData)
+       ordering <- sample(headerList, length(headerList)) 
+    } else {
+       headerList <- colnames(matrixData)
+       ordering <- sample(headerList, length(headerList)) 
+    }
+  }
+  else if (orderMethod == "Original")
+  {
+    if(direction=="row") {
+       ordering <- rownames(matrixData) 
+    } else {
+       ordering <- colnames(matrixData) 
+    }
+  } else {
+    stop("createOrdering -- failed to find ordering method")
+  }
+  return(ordering)
+}
+### Initialize command line arguments and call performDataOrdering
+
+options(warn=-1)
+
+args = commandArgs(TRUE)
+
+performDataOrdering(dataFile=args[1], rowOrderMethod=args[2], rowDistanceMeasure=args[3], rowAgglomerationMethod=args[4], colOrderMethod=args[5], colDistanceMeasure=args[6], colAgglomerationMethod=args[7],rowOrderFile=args[8], colOrderFile=args[9], rowDendroFile=args[10], colDendroFile=args[11], rowCut=args[12], colCut=args[13])
+
+#suppressWarnings(performDataOrdering(dataFile=args[1], rowOrderMethod=args[2], rowDistanceMeasure=args[3], rowAgglomerationMethod=args[4], colOrderMethod=args[5], colDistanceMeasure=args[6], colAgglomerationMethod=args[7],rowOrderFile=args[8], colOrderFile=args[9], rowDendroFile=args[10], colDendroFile=args[11]))
Binary file GalaxyMapGen.jar has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/heatmap.sh	Thu Jan 26 10:15:31 2017 -0500
@@ -0,0 +1,47 @@
+echo $1 $2 $3 $4 $5 $6 $7 $8 $9 ${10} ${11} ${12} ${13} ${14} ${15} ${16} ${17}
+#create temp directory for row and col order and dendro files.
+tdir=${11}/$(date +%y%m%d%M%S)
+echo $tdir
+mkdir $tdir
+#run R to cluster matrix
+output="$(R --slave --vanilla --file=${11}/CHM.R --args $3 $4 $5 $6 $7 $8 $9 $tdir/ROfile.txt $tdir/COfile.txt $tdir/RDfile.txt $tdir/CDfile.txt ${12} ${13} ${14} ${15} 2>&1)"
+rc=$?;
+if [ $rc != 0 ]
+then
+  echo $output;
+  if [ `echo "$output" | grep -c "Inf in foreign function call"` -gt 0 ]
+  then
+    echo "";
+    echo "Note: This error can occur when there is no variation in a row or column.  Try a different distance measure or remove rows/columns without variation.";
+  fi
+  exit $rc;
+fi
+
+#there are a variable number of triplicate parameters for classification bars
+count=0
+classifications=''
+
+#if row cut was done, add that autogenerated classification
+if [ ${12} -gt 1 ]
+then
+  classifications="Class $tdir/ROfile.txt.cut row_categorical"
+fi
+
+#if col cut was done, add that autogenerated classification
+if [ ${13} -gt 1 ]
+then
+  classifications="$classifications Class $tdir/COfile.txt.cut col_categorical"
+fi
+
+#now add the user provided classification files 
+for i in "$@"; do
+  if [ $count -gt 14 ]
+  then
+    classifications=$classifications' '$i
+  fi
+  count=$((count+1))
+done
+#call java program to generate NGCHM viewer files.
+java -jar ${11}/GalaxyMapGen.jar "${1}" "${2}" DataLayer1 $3 linear ${14} ${15} $4 $5 $6 $tdir/ROfile.txt $tdir/RDfile.txt $7 $8 $9 $tdir/COfile.txt $tdir/CDfile.txt ${10} $classifications
+#clean up tempdir
+rm -rf $tdir
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/mda_heatmap_gen.xml	Thu Jan 26 10:15:31 2017 -0500
@@ -0,0 +1,204 @@
+<?xml version="1.0" encoding="UTF-8" ?>
+<tool id="mda_heatmap_gen" name="NG-CHM Generator" version="6.0.2">
+  <description>Create Clustered Heat Maps</description>
+<command interpreter="bash" detect_errors="aggressive">$__tool_directory__/heatmap.sh  '$hmname' '$hmdesc' '$inputmatrix' ${d_rows.rowOrderMethod} ${d_rows.rowDistanceMeasure} ${d_rows.rowAgglomerationMethod} ${d_cols.columnOrderMethod} ${d_cols.columnDistanceMeasure} ${d_cols.columnAgglomerationMethod} $summarymethod '$__tool_directory__' ${d_rows.rowDendroCut} ${d_cols.colDendroCut} $rowDataType $colDataType
+    #for $op in $operations
+       ${op.class_name}
+       ${op.repeatinput.file_name}
+       ${op.cat}
+      #end for
+ 	'$output' 
+ </command>
+	<stdio>
+      <exit_code range="1:" level="fatal" />
+	</stdio>
+  <inputs>
+    <param name="inputmatrix" type="data" label="Input Matrix" />
+    <param name="hmname" size="20" type="text" value="Heat_Map_name" label="User Defined Heat Map Name"/>
+    <param name="hmdesc" size="100" optional="true" type="text" value="Heat_Map_description" label="User Defined Heat Map Description"/>
+    <param name="summarymethod" 	type="select"  label="Data Summarization Method">
+		<option value="average">average</option>
+		<option value="sample">sample</option>
+		<option value="mode">mode</option>
+    </param>
+      <conditional name="d_rows">
+	<param name="rowOrderMethod" type="select" label="Row ordering method" help="Choices -- Hierarchical Clustering, Original Order, Random">
+		<option value="Hierarchical">Hierarchical Clustering</option>
+		<option value="Original">Original Order</option>	    
+		<option value="Random">Random</option>	    
+	</param>
+        <when value="Hierarchical">
+	<param name="rowDistanceMeasure" type="select"  label="Row Distance Metric" help="euclidean, binary, manhattan, maximum, canberra, minkowski, or correlation">
+		<option value="euclidean">Euclidean</option>
+		<option value="binary">Binary</option>
+		<option value="manhattan">Manhattan</option>
+		<option value="maximum">Maximum</option>
+		<option value="canberra">Canberra</option>	    
+		<option value="minkowski">Minkowski</option>	    
+		<option value="correlation">Correlation</option>	    
+	</param>
+	<param name="rowAgglomerationMethod" type="select"  label="Row Clustering Method" help="Choices: 'average' for Average Linkage, 'complete' for Complete Linkage, 'single' for Single Linkage, 'ward', 'mcquitty', 'median', or 'centroid'.">
+		<option value="average">Average Linkage</option>
+		<option value="complete">Complete Linkage</option>
+		<option value="single">Single Linkage</option>
+		<option value="ward" selected="true">Ward</option>
+		<option value="mcquitty">Mcquitty</option>	    
+		<option value="median">Median</option>	    
+		<option value="centroid">Centroid</option>	    
+	</param>
+            <param name="rowDendroCut" type="select" label="Create row categorical covariate bar based on number of top-level dendrogram clusters" >
+        		<option value="0" selected="true" >None</option>
+        		<option value="2" >2</option>
+        		<option value="3" >3</option>
+        		<option value="4" >4</option>
+        		<option value="5" >5</option>
+        		<option value="6" >6</option>
+        		<option value="7" >7</option>
+        		<option value="8" >8</option>
+        		<option value="9" >9</option>
+        		<option value="10" >10</option>
+            </param>
+        </when>
+        <when value="Original">
+		    <param name="rowDistanceMeasure" type="text" size="0"     value="n/a"/>
+		    <param name="rowAgglomerationMethod" type="text" size="0"     value="n/a"/>
+		    <param name="rowDendroCut" size="0"   type="text" value="0"/>
+        </when>
+        <when value="Random">
+		    <param name="rowDistanceMeasure" type="text" size="0"     value="n/a"/>
+		    <param name="rowAgglomerationMethod" type="text" size="0"     value="n/a"/>
+		    <param name="rowDendroCut" type="text" size="0"    value="0"/>
+        </when>
+    </conditional>
+    <param name="rowDataType" type="select" label="Linkouts to row data type info" >
+        <option value="labels" selected="true" >None</option>
+        <option value="bio.probe.affymetrix" >Affymetrix Probe Id</option>
+        <option value="bio.feature.agilent" >Agilent Id</option>
+        <option value="bio.sample.cbioportal" >cBioPortal sample Id</option>
+        <option value="bio.transcript.ensemble" >Ensemble transcript Id</option>
+        <option value="bio.gene.entrez" >Gene Entrez Id</option>
+        <option value="bio.gene.hugo" >Gene HUGO symbol</option>
+        <option value="bio.go" >Gene Ontology (GO) Id</option>
+        <option value="bio.geo.acc" >GEO Accession Id</option>
+        <option value="bio.probe.illumina" >Illumina Probe Id</option>
+        <option value="bio.probe.infinium" >Infinium Probe Id</option>
+        <option value="bio.pathway.mdanderson" >MD Anderson pathway Id</option>
+        <option value="bio.mirna" >miRNA Id</option>
+        <option value="bio.mirna.mimat" >miRNA MIMAT Id</option>
+        <option value="bio.pubmed" >Pubmed Id</option>
+        <option value="bio.pubmed.search" >Pubmed Search Term</option>
+        <option value="scholar" >Scholarly term</option>
+        <option value="bio.gene.unigene" >Unigene CID</option>
+        <option value="bio.protein.uniprot" >UniProt Id</option>
+    </param>    
+	<conditional name="d_cols">
+	<param name="columnOrderMethod" type="select" label="Column ordering method" help="Choices -- Hierarchical Clustering, Original Order, Random">
+		<option value="Hierarchical">Hierarchical Clustering</option>
+		<option value="Original">Original Order</option>	    
+		<option value="Random">Random</option>	    
+	</param>
+        <when value="Hierarchical">
+	<param name="columnDistanceMeasure" type="select"  label="Column Distance Metric" help="euclidean, binary, manhattan, maximum, canberra, minkowski, or correlation">
+		<option value="euclidean">Euclidean</option>
+		<option value="binary">Binary</option>
+		<option value="manhattan">Manhattan</option>
+		<option value="maximum">Maximum</option>
+		<option value="canberra">Canberra</option>	    
+		<option value="minkowski">Minkowski</option>	    
+		<option value="correlation">Correlation</option>	    
+	</param>
+	<param name="columnAgglomerationMethod" type="select"  label="Column Clustering Method" help="Choices: 'average' for Average Linkage, 'complete' for Complete Linkage, 'single' for Single Linkage, 'ward', 'mcquitty', 'median', or 'centroid'.">
+		<option value="average">Average Linkage</option>
+		<option value="complete">Complete Linkage</option>
+		<option value="single">Single Linkage</option>
+		<option value="ward" selected="true">Ward</option>
+		<option value="mcquitty">Mcquitty</option>	    
+		<option value="median">Median</option>	    
+		<option value="centroid">Centroid</option>	    
+	</param>
+            <param name="colDendroCut" type="select" label="Create column categorical covariate bar based on number of top-level dendrogram clusters" >
+        		<option value="0" selected="true" >None</option>
+        		<option value="2" >2</option>
+        		<option value="3" >3</option>
+        		<option value="4" >4</option>
+        		<option value="5" >5</option>
+        		<option value="6" >6</option>
+        		<option value="7" >7</option>
+        		<option value="8" >8</option>
+        		<option value="9" >9</option>
+        		<option value="10" >10</option>
+            </param>
+        </when>
+        <when value="Original">
+		    <param name="columnDistanceMeasure" type="text" size="0"     value="n/a"/>
+		    <param name="columnAgglomerationMethod" type="text" size="0"     value="n/a"/>
+		    <param name="colDendroCut" type="text" size="0"     value="0"/>
+        </when>
+        <when value="Random">
+		    <param name="columnDistanceMeasure" type="text" size="0"     value="n/a"/>
+		    <param name="columnAgglomerationMethod" type="text" size="0"     value="n/a"/>
+		    <param name="colDendroCut" type="text" size="0"     value="0"/>
+        </when>
+    </conditional>
+    <param name="colDataType" type="select" label="Linkouts to column data type info" >
+        <option value="labels" selected="true" >None</option>
+        <option value="bio.probe.affymetrix" >Affymetrix Probe Id</option>
+        <option value="bio.feature.agilent" >Agilent Id</option>
+        <option value="bio.sample.cbioportal" >cBioPortal sample Id</option>
+        <option value="bio.transcript.ensemble" >Ensemble transcript Id</option>
+        <option value="bio.gene.entrez" >Gene Entrez Id</option>
+        <option value="bio.gene.hugo" >Gene HUGO symbol</option>
+        <option value="bio.go" >Gene Ontology (GO) Id</option>
+        <option value="bio.geo.acc" >GEO Accession Id</option>
+        <option value="bio.probe.illumina" >Illumina Probe Id</option>
+        <option value="bio.probe.infinium" >Infinium Probe Id</option>
+        <option value="bio.pathway.mdanderson" >MD Anderson pathway Id</option>
+        <option value="bio.mirna" >miRNA Id</option>
+        <option value="bio.mirna.mimat" >miRNA MIMAT Id</option>
+        <option value="bio.pubmed" >Pubmed Id</option>
+        <option value="bio.pubmed.search" >Pubmed Search Term</option>
+        <option value="scholar" >Scholarly term</option>
+        <option value="bio.gene.unigene" >Unigene CId</option>
+        <option value="bio.protein.uniprot" >UniProt Id</option>
+    </param>    
+    <repeat name="operations" title="Covariate Bars">
+        <param name="class_name" size="20" type="text" value="" label="Axis Covariate Name">
+           <sanitizer invalid_char="_">
+              <valid initial="none">
+                <add preset="string.letters"/>
+                <add preset="string.digits"/>
+              </valid>
+              <mapping initial="none">
+              </mapping>
+           </sanitizer>
+        </param>
+        <param name="repeatinput" type="data" format="text" label="Axis Covariate File"/>
+	<param name="cat" type="select" label="Axis Covariate Type">
+	  <option value="row_categorical" >row categorical</option>
+	  <option value="row_continuous" >row continuous</option>
+	  <option value="column_categorical" >column categorical</option>
+	  <option value="column_continuous" >column continuous</option>
+	</param>
+    </repeat>       
+  </inputs>
+  <outputs>
+    <data name="output" label='${hmname}' format="ngchm"/>
+  </outputs>
+ <tests>
+    <test>
+      <param name="inputmatrix" value="400x400.txt" />
+      <param name="hmname" value="testRun" />
+      <param name="$hmdesc" value="validateTool" />
+      <param name="summarymethod" value="average" />
+      <param name="rowOrderMethod" value="Hierarchical" />
+      <param name="rowDistanceMeasure" value="manhattan" />
+      <param name="rowAgglomerationMethod" value="ward" />
+      <param name="columnOrderMethod" value="Hierarchical" />
+      <param name="columnDistanceMeasure" value="manhattan" />
+      <param name="columnAgglomerationMethod" value="ward" />
+      <output name="output" file="Galaxy400x400-noCovariates.ngchm" lines_diff="10" />     
+
+    </test>
+<!--   galaxy/test-data/    dir where the input and output file that should match tool output will be copied -->
+  </tests>
+ </tool>
Binary file mda_heatmap_viz.zip has changed
Binary file ngchm-matrix-functional-test-data/._.DS_Store has changed
Binary file ngchm-matrix-functional-test-data/._400x400.txt has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/ngchm-matrix-functional-test-data/400x400-column-covariate.txt	Thu Jan 26 10:15:31 2017 -0500
@@ -0,0 +1,1 @@
+TCGA_SAMP_1	1111
TCGA_SAMP_2	1111
TCGA_SAMP_3	1111
TCGA_SAMP_4	1111
TCGA_SAMP_5	1111
TCGA_SAMP_6	1111
TCGA_SAMP_7	1111
TCGA_SAMP_8	1111
TCGA_SAMP_9	1111
TCGA_SAMP_10	1111
TCGA_SAMP_11	1111
TCGA_SAMP_12	1111
TCGA_SAMP_13	1111
TCGA_SAMP_14	1111
TCGA_SAMP_15	1111
TCGA_SAMP_16	1111
TCGA_SAMP_17	1111
TCGA_SAMP_18	1111
TCGA_SAMP_19	1111
TCGA_SAMP_20	1111
TCGA_SAMP_21	1111
TCGA_SAMP_22	22
TCGA_SAMP_23	22
TCGA_SAMP_24	22
TCGA_SAMP_25	22
TCGA_SAMP_26	22
TCGA_SAMP_27	22
TCGA_SAMP_28	22
TCGA_SAMP_29	22
TCGA_SAMP_30	22
TCGA_SAMP_31	22
TCGA_SAMP_32	22
TCGA_SAMP_33	22
TCGA_SAMP_34	22
TCGA_SAMP_35	22
TCGA_SAMP_36	22
TCGA_SAMP_37	333
TCGA_SAMP_38	333
TCGA_SAMP_39	333
TCGA_SAMP_40	333
TCGA_SAMP_41	333
TCGA_SAMP_42	333
TCGA_SAMP_43	333
TCGA_SAMP_44	333
TCGA_SAMP_45	333
TCGA_SAMP_46	333
TCGA_SAMP_47	333
TCGA_SAMP_48	333
TCGA_SAMP_49	333
TCGA_SAMP_50	333
TCGA_SAMP_51	1111
TCGA_SAMP_52	1111
TCGA_SAMP_53	1111
TCGA_SAMP_54	1111
TCGA_SAMP_55	1111
TCGA_SAMP_56	1111
TCGA_SAMP_57	1111
TCGA_SAMP_58	1111
TCGA_SAMP_59	1111
TCGA_SAMP_60	1111
TCGA_SAMP_61	1111
TCGA_SAMP_62	1111
TCGA_SAMP_63	1111
TCGA_SAMP_64	1111
TCGA_SAMP_65	1111
TCGA_SAMP_66	1111
TCGA_SAMP_67	1111
TCGA_SAMP_68	1111
TCGA_SAMP_69	1111
TCGA_SAMP_70	1111
TCGA_SAMP_71	1111
TCGA_SAMP_72	22
TCGA_SAMP_73	22
TCGA_SAMP_74	22
TCGA_SAMP_75	22
TCGA_SAMP_76	22
TCGA_SAMP_77	22
TCGA_SAMP_78	22
TCGA_SAMP_79	22
TCGA_SAMP_80	22
TCGA_SAMP_81	22
TCGA_SAMP_82	22
TCGA_SAMP_83	22
TCGA_SAMP_84	22
TCGA_SAMP_85	22
TCGA_SAMP_86	22
TCGA_SAMP_87	333
TCGA_SAMP_88	333
TCGA_SAMP_89	333
TCGA_SAMP_90	333
TCGA_SAMP_91	333
TCGA_SAMP_92	333
TCGA_SAMP_93	333
TCGA_SAMP_94	333
TCGA_SAMP_95	333
TCGA_SAMP_96	333
TCGA_SAMP_97	333
TCGA_SAMP_98	333
TCGA_SAMP_99	333
TCGA_SAMP_100	333
TCGA_SAMP_101	1111
TCGA_SAMP_102	1111
TCGA_SAMP_103	1111
TCGA_SAMP_104	1111
TCGA_SAMP_105	1111
TCGA_SAMP_106	1111
TCGA_SAMP_107	1111
TCGA_SAMP_108	1111
TCGA_SAMP_109	1111
TCGA_SAMP_110	1111
TCGA_SAMP_111	1111
TCGA_SAMP_112	1111
TCGA_SAMP_113	1111
TCGA_SAMP_114	1111
TCGA_SAMP_115	1111
TCGA_SAMP_116	1111
TCGA_SAMP_117	1111
TCGA_SAMP_118	1111
TCGA_SAMP_119	1111
TCGA_SAMP_120	1111
TCGA_SAMP_121	1111
TCGA_SAMP_122	22
TCGA_SAMP_123	22
TCGA_SAMP_124	22
TCGA_SAMP_125	22
TCGA_SAMP_126	22
TCGA_SAMP_127	22
TCGA_SAMP_128	22
TCGA_SAMP_129	22
TCGA_SAMP_130	22
TCGA_SAMP_131	22
TCGA_SAMP_132	22
TCGA_SAMP_133	22
TCGA_SAMP_134	22
TCGA_SAMP_135	22
TCGA_SAMP_136	22
TCGA_SAMP_137	333
TCGA_SAMP_138	333
TCGA_SAMP_139	333
TCGA_SAMP_140	333
TCGA_SAMP_141	333
TCGA_SAMP_142	333
TCGA_SAMP_143	333
TCGA_SAMP_144	333
TCGA_SAMP_145	333
TCGA_SAMP_146	333
TCGA_SAMP_147	333
TCGA_SAMP_148	333
TCGA_SAMP_149	333
TCGA_SAMP_150	333
TCGA_SAMP_151	1111
TCGA_SAMP_152	1111
TCGA_SAMP_153	1111
TCGA_SAMP_154	1111
TCGA_SAMP_155	1111
TCGA_SAMP_156	1111
TCGA_SAMP_157	1111
TCGA_SAMP_158	1111
TCGA_SAMP_159	1111
TCGA_SAMP_160	1111
TCGA_SAMP_161	1111
TCGA_SAMP_162	1111
TCGA_SAMP_163	1111
TCGA_SAMP_164	1111
TCGA_SAMP_165	1111
TCGA_SAMP_166	1111
TCGA_SAMP_167	1111
TCGA_SAMP_168	1111
TCGA_SAMP_169	1111
TCGA_SAMP_170	1111
TCGA_SAMP_171	1111
TCGA_SAMP_172	22
TCGA_SAMP_173	22
TCGA_SAMP_174	22
TCGA_SAMP_175	22
TCGA_SAMP_176	22
TCGA_SAMP_177	22
TCGA_SAMP_178	22
TCGA_SAMP_179	22
TCGA_SAMP_180	22
TCGA_SAMP_181	22
TCGA_SAMP_182	22
TCGA_SAMP_183	22
TCGA_SAMP_184	22
TCGA_SAMP_185	22
TCGA_SAMP_186	22
TCGA_SAMP_187	333
TCGA_SAMP_188	333
TCGA_SAMP_189	333
TCGA_SAMP_190	333
TCGA_SAMP_191	333
TCGA_SAMP_192	333
TCGA_SAMP_193	333
TCGA_SAMP_194	333
TCGA_SAMP_195	333
TCGA_SAMP_196	333
TCGA_SAMP_197	333
TCGA_SAMP_198	333
TCGA_SAMP_199	333
TCGA_SAMP_200	333
TCGA_SAMP_201	1111
TCGA_SAMP_202	1111
TCGA_SAMP_203	1111
TCGA_SAMP_204	1111
TCGA_SAMP_205	1111
TCGA_SAMP_206	1111
TCGA_SAMP_207	1111
TCGA_SAMP_208	1111
TCGA_SAMP_209	1111
TCGA_SAMP_210	1111
TCGA_SAMP_211	1111
TCGA_SAMP_212	1111
TCGA_SAMP_213	1111
TCGA_SAMP_214	1111
TCGA_SAMP_215	1111
TCGA_SAMP_216	1111
TCGA_SAMP_217	1111
TCGA_SAMP_218	1111
TCGA_SAMP_219	1111
TCGA_SAMP_220	1111
TCGA_SAMP_221	1111
TCGA_SAMP_222	22
TCGA_SAMP_223	22
TCGA_SAMP_224	22
TCGA_SAMP_225	22
TCGA_SAMP_226	22
TCGA_SAMP_227	22
TCGA_SAMP_228	22
TCGA_SAMP_229	22
TCGA_SAMP_230	22
TCGA_SAMP_231	22
TCGA_SAMP_232	22
TCGA_SAMP_233	22
TCGA_SAMP_234	22
TCGA_SAMP_235	22
TCGA_SAMP_236	22
TCGA_SAMP_237	333
TCGA_SAMP_238	333
TCGA_SAMP_239	333
TCGA_SAMP_240	333
TCGA_SAMP_241	333
TCGA_SAMP_242	333
TCGA_SAMP_243	333
TCGA_SAMP_244	333
TCGA_SAMP_245	333
TCGA_SAMP_246	333
TCGA_SAMP_247	333
TCGA_SAMP_248	333
TCGA_SAMP_249	333
TCGA_SAMP_250	333
TCGA_SAMP_251	1111
TCGA_SAMP_252	1111
TCGA_SAMP_253	1111
TCGA_SAMP_254	1111
TCGA_SAMP_255	1111
TCGA_SAMP_256	1111
TCGA_SAMP_257	1111
TCGA_SAMP_258	1111
TCGA_SAMP_259	1111
TCGA_SAMP_260	1111
TCGA_SAMP_261	1111
TCGA_SAMP_262	1111
TCGA_SAMP_263	1111
TCGA_SAMP_264	1111
TCGA_SAMP_265	1111
TCGA_SAMP_266	1111
TCGA_SAMP_267	1111
TCGA_SAMP_268	1111
TCGA_SAMP_269	1111
TCGA_SAMP_270	1111
TCGA_SAMP_271	1111
TCGA_SAMP_272	22
TCGA_SAMP_273	22
TCGA_SAMP_274	22
TCGA_SAMP_275	22
TCGA_SAMP_276	22
TCGA_SAMP_277	22
TCGA_SAMP_278	22
TCGA_SAMP_279	22
TCGA_SAMP_280	22
TCGA_SAMP_281	22
TCGA_SAMP_282	22
TCGA_SAMP_283	22
TCGA_SAMP_284	22
TCGA_SAMP_285	22
TCGA_SAMP_286	22
TCGA_SAMP_287	333
TCGA_SAMP_288	333
TCGA_SAMP_289	333
TCGA_SAMP_290	333
TCGA_SAMP_291	333
TCGA_SAMP_292	333
TCGA_SAMP_293	333
TCGA_SAMP_294	333
TCGA_SAMP_295	333
TCGA_SAMP_296	333
TCGA_SAMP_297	333
TCGA_SAMP_298	333
TCGA_SAMP_299	333
TCGA_SAMP_300	333
TCGA_SAMP_301	1111
TCGA_SAMP_302	1111
TCGA_SAMP_303	1111
TCGA_SAMP_304	1111
TCGA_SAMP_305	1111
TCGA_SAMP_306	1111
TCGA_SAMP_307	1111
TCGA_SAMP_308	1111
TCGA_SAMP_309	1111
TCGA_SAMP_310	1111
TCGA_SAMP_311	1111
TCGA_SAMP_312	1111
TCGA_SAMP_313	1111
TCGA_SAMP_314	1111
TCGA_SAMP_315	1111
TCGA_SAMP_316	1111
TCGA_SAMP_317	1111
TCGA_SAMP_318	1111
TCGA_SAMP_319	1111
TCGA_SAMP_320	1111
TCGA_SAMP_321	1111
TCGA_SAMP_322	22
TCGA_SAMP_323	22
TCGA_SAMP_324	22
TCGA_SAMP_325	22
TCGA_SAMP_326	22
TCGA_SAMP_327	22
TCGA_SAMP_328	22
TCGA_SAMP_329	22
TCGA_SAMP_330	22
TCGA_SAMP_331	22
TCGA_SAMP_332	22
TCGA_SAMP_333	22
TCGA_SAMP_334	22
TCGA_SAMP_335	22
TCGA_SAMP_336	22
TCGA_SAMP_337	333
TCGA_SAMP_338	333
TCGA_SAMP_339	333
TCGA_SAMP_340	333
TCGA_SAMP_341	333
TCGA_SAMP_342	333
TCGA_SAMP_343	333
TCGA_SAMP_344	333
TCGA_SAMP_345	333
TCGA_SAMP_346	333
TCGA_SAMP_347	333
TCGA_SAMP_348	333
TCGA_SAMP_349	333
TCGA_SAMP_350	333
TCGA_SAMP_351	1111
TCGA_SAMP_352	1111
TCGA_SAMP_353	1111
TCGA_SAMP_354	1111
TCGA_SAMP_355	1111
TCGA_SAMP_356	1111
TCGA_SAMP_357	1111
TCGA_SAMP_358	1111
TCGA_SAMP_359	1111
TCGA_SAMP_360	1111
TCGA_SAMP_361	1111
TCGA_SAMP_362	1111
TCGA_SAMP_363	1111
TCGA_SAMP_364	1111
TCGA_SAMP_365	1111
TCGA_SAMP_366	1111
TCGA_SAMP_367	1111
TCGA_SAMP_368	1111
TCGA_SAMP_369	1111
TCGA_SAMP_370	1111
TCGA_SAMP_371	1111
TCGA_SAMP_372	22
TCGA_SAMP_373	22
TCGA_SAMP_374	22
TCGA_SAMP_375	22
TCGA_SAMP_376	22
TCGA_SAMP_377	22
TCGA_SAMP_378	22
TCGA_SAMP_379	22
TCGA_SAMP_380	22
TCGA_SAMP_381	22
TCGA_SAMP_382	22
TCGA_SAMP_383	22
TCGA_SAMP_384	22
TCGA_SAMP_385	22
TCGA_SAMP_386	22
TCGA_SAMP_387	333
TCGA_SAMP_388	333
TCGA_SAMP_389	333
TCGA_SAMP_390	333
TCGA_SAMP_391	333
TCGA_SAMP_392	333
TCGA_SAMP_393	333
TCGA_SAMP_394	333
TCGA_SAMP_395	333
TCGA_SAMP_396	333
TCGA_SAMP_397	333
TCGA_SAMP_398	333
TCGA_SAMP_399	333
TCGA_SAMP_400	333
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
	
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Gene_300	bb
Gene_301	bb
Gene_302	bb
Gene_303	bb
Gene_304	bb
Gene_305	bb
Gene_306	bb
Gene_307	bb
Gene_308	bb
Gene_309	bb
Gene_310	bb
Gene_311	bb
Gene_312	cccc
Gene_313	cccc
Gene_314	cccc
Gene_315	cccc
Gene_316	cccc
Gene_317	cccc
Gene_318	cccc
Gene_319	cccc
Gene_320	cccc
Gene_321	cccc
Gene_322	cccc
Gene_323	cccc
Gene_324	cccc
Gene_325	cccc
Gene_326	cccc
Gene_327	cccc
Gene_328	cccc
Gene_329	aaa
Gene_330	aaa
Gene_331	aaa
Gene_332	aaa
Gene_333	aaa
Gene_334	aaa
Gene_335	bb
Gene_336	bb
Gene_337	aaa
Gene_338	aaa
Gene_339	aaa
Gene_340	aaa
Gene_341	aaa
Gene_342	aaa
Gene_343	bb
Gene_344	bb
Gene_345	bb
Gene_346	bb
Gene_347	bb
Gene_348	bb
Gene_349	bb
Gene_350	bb
Gene_351	bb
Gene_352	bb
Gene_353	bb
Gene_354	bb
Gene_355	bb
Gene_356	bb
Gene_357	bb
Gene_358	bb
Gene_359	bb
Gene_360	bb
Gene_361	bb
Gene_362	bb
Gene_363	bb
Gene_364	bb
Gene_365	bb
Gene_366	bb
Gene_367	bb
Gene_368	cccc
Gene_369	cccc
Gene_370	cccc
Gene_371	cccc
Gene_372	cccc
Gene_373	cccc
Gene_374	cccc
Gene_375	cccc
Gene_376	cccc
Gene_377	cccc
Gene_378	cccc
Gene_379	cccc
Gene_380	cccc
Gene_381	cccc
Gene_382	cccc
Gene_383	cccc
Gene_384	cccc
Gene_385	aaa
Gene_386	aaa
Gene_387	aaa
Gene_388	aaa
Gene_389	aaa
Gene_390	aaa
Gene_391	bb
Gene_392	bb
Gene_393	aaa
Gene_394	aaa
Gene_395	aaa
Gene_396	aaa
Gene_397	aaa
Gene_398	aaa
Gene_399	bb
Gene_400	bb
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/ngchm-matrix-functional-test-data/400x400.txt	Thu Jan 26 10:15:31 2017 -0500
@@ -0,0 +1,401 @@
+	TCGA_SAMP_1	TCGA_SAMP_2	TCGA_SAMP_3	TCGA_SAMP_4	TCGA_SAMP_5	TCGA_SAMP_6	TCGA_SAMP_7	TCGA_SAMP_8	TCGA_SAMP_9	TCGA_SAMP_10	TCGA_SAMP_11	TCGA_SAMP_12	TCGA_SAMP_13	TCGA_SAMP_14	TCGA_SAMP_15	TCGA_SAMP_16	TCGA_SAMP_17	TCGA_SAMP_18	TCGA_SAMP_19	TCGA_SAMP_20	TCGA_SAMP_21	TCGA_SAMP_22	TCGA_SAMP_23	TCGA_SAMP_24	TCGA_SAMP_25	TCGA_SAMP_26	TCGA_SAMP_27	TCGA_SAMP_28	TCGA_SAMP_29	TCGA_SAMP_30	TCGA_SAMP_31	TCGA_SAMP_32	TCGA_SAMP_33	TCGA_SAMP_34	TCGA_SAMP_35	TCGA_SAMP_36	TCGA_SAMP_37	TCGA_SAMP_38	TCGA_SAMP_39	TCGA_SAMP_40	TCGA_SAMP_41	TCGA_SAMP_42	TCGA_SAMP_43	TCGA_SAMP_44	TCGA_SAMP_45	TCGA_SAMP_46	TCGA_SAMP_47	TCGA_SAMP_48	TCGA_SAMP_49	TCGA_SAMP_50	TCGA_SAMP_51	TCGA_SAMP_52	TCGA_SAMP_53	TCGA_SAMP_54	TCGA_SAMP_55	TCGA_SAMP_56	TCGA_SAMP_57	TCGA_SAMP_58	TCGA_SAMP_59	TCGA_SAMP_60	TCGA_SAMP_61	TCGA_SAMP_62	TCGA_SAMP_63	TCGA_SAMP_64	TCGA_SAMP_65	TCGA_SAMP_66	TCGA_SAMP_67	TCGA_SAMP_68	TCGA_SAMP_69	TCGA_SAMP_70	TCGA_SAMP_71	TCGA_SAMP_72	TCGA_SAMP_73	TCGA_SAMP_74	TCGA_SAMP_75	TCGA_SAMP_76	TCGA_SAMP_77	TCGA_SAMP_78	TCGA_SAMP_79	TCGA_SAMP_80	TCGA_SAMP_81	TCGA_SAMP_82	TCGA_SAMP_83	TCGA_SAMP_84	TCGA_SAMP_85	TCGA_SAMP_86	TCGA_SAMP_87	TCGA_SAMP_88	TCGA_SAMP_89	TCGA_SAMP_90	TCGA_SAMP_91	TCGA_SAMP_92	TCGA_SAMP_93	TCGA_SAMP_94	TCGA_SAMP_95	TCGA_SAMP_96	TCGA_SAMP_97	TCGA_SAMP_98	TCGA_SAMP_99	TCGA_SAMP_100	TCGA_SAMP_101	TCGA_SAMP_102	TCGA_SAMP_103	TCGA_SAMP_104	TCGA_SAMP_105	TCGA_SAMP_106	TCGA_SAMP_107	TCGA_SAMP_108	TCGA_SAMP_109	TCGA_SAMP_110	TCGA_SAMP_111	TCGA_SAMP_112	TCGA_SAMP_113	TCGA_SAMP_114	TCGA_SAMP_115	TCGA_SAMP_116	TCGA_SAMP_117	TCGA_SAMP_118	TCGA_SAMP_119	TCGA_SAMP_120	TCGA_SAMP_121	TCGA_SAMP_122	TCGA_SAMP_123	TCGA_SAMP_124	TCGA_SAMP_125	TCGA_SAMP_126	TCGA_SAMP_127	TCGA_SAMP_128	TCGA_SAMP_129	TCGA_SAMP_130	TCGA_SAMP_131	TCGA_SAMP_132	TCGA_SAMP_133	TCGA_SAMP_134	TCGA_SAMP_135	TCGA_SAMP_136	TCGA_SAMP_137	TCGA_SAMP_138	TCGA_SAMP_139	TCGA_SAMP_140	TCGA_SAMP_141	TCGA_SAMP_142	TCGA_SAMP_143	TCGA_SAMP_144	TCGA_SAMP_145	TCGA_SAMP_146	TCGA_SAMP_147	TCGA_SAMP_148	TCGA_SAMP_149	TCGA_SAMP_150	TCGA_SAMP_151	TCGA_SAMP_152	TCGA_SAMP_153	TCGA_SAMP_154	TCGA_SAMP_155	TCGA_SAMP_156	TCGA_SAMP_157	TCGA_SAMP_158	TCGA_SAMP_159	TCGA_SAMP_160	TCGA_SAMP_161	TCGA_SAMP_162	TCGA_SAMP_163	TCGA_SAMP_164	TCGA_SAMP_165	TCGA_SAMP_166	TCGA_SAMP_167	TCGA_SAMP_168	TCGA_SAMP_169	TCGA_SAMP_170	TCGA_SAMP_171	TCGA_SAMP_172	TCGA_SAMP_173	TCGA_SAMP_174	TCGA_SAMP_175	TCGA_SAMP_176	TCGA_SAMP_177	TCGA_SAMP_178	TCGA_SAMP_179	TCGA_SAMP_180	TCGA_SAMP_181	TCGA_SAMP_182	TCGA_SAMP_183	TCGA_SAMP_184	TCGA_SAMP_185	TCGA_SAMP_186	TCGA_SAMP_187	TCGA_SAMP_188	TCGA_SAMP_189	TCGA_SAMP_190	TCGA_SAMP_191	TCGA_SAMP_192	TCGA_SAMP_193	TCGA_SAMP_194	TCGA_SAMP_195	TCGA_SAMP_196	TCGA_SAMP_197	TCGA_SAMP_198	TCGA_SAMP_199	TCGA_SAMP_200	TCGA_SAMP_201	TCGA_SAMP_202	TCGA_SAMP_203	TCGA_SAMP_204	TCGA_SAMP_205	TCGA_SAMP_206	TCGA_SAMP_207	TCGA_SAMP_208	TCGA_SAMP_209	TCGA_SAMP_210	TCGA_SAMP_211	TCGA_SAMP_212	TCGA_SAMP_213	TCGA_SAMP_214	TCGA_SAMP_215	TCGA_SAMP_216	TCGA_SAMP_217	TCGA_SAMP_218	TCGA_SAMP_219	TCGA_SAMP_220	TCGA_SAMP_221	TCGA_SAMP_222	TCGA_SAMP_223	TCGA_SAMP_224	TCGA_SAMP_225	TCGA_SAMP_226	TCGA_SAMP_227	TCGA_SAMP_228	TCGA_SAMP_229	TCGA_SAMP_230	TCGA_SAMP_231	TCGA_SAMP_232	TCGA_SAMP_233	TCGA_SAMP_234	TCGA_SAMP_235	TCGA_SAMP_236	TCGA_SAMP_237	TCGA_SAMP_238	TCGA_SAMP_239	TCGA_SAMP_240	TCGA_SAMP_241	TCGA_SAMP_242	TCGA_SAMP_243	TCGA_SAMP_244	TCGA_SAMP_245	TCGA_SAMP_246	TCGA_SAMP_247	TCGA_SAMP_248	TCGA_SAMP_249	TCGA_SAMP_250	TCGA_SAMP_251	TCGA_SAMP_252	TCGA_SAMP_253	TCGA_SAMP_254	TCGA_SAMP_255	TCGA_SAMP_256	TCGA_SAMP_257	TCGA_SAMP_258	TCGA_SAMP_259	TCGA_SAMP_260	TCGA_SAMP_261	TCGA_SAMP_262	TCGA_SAMP_263	TCGA_SAMP_264	TCGA_SAMP_265	TCGA_SAMP_266	TCGA_SAMP_267	TCGA_SAMP_268	TCGA_SAMP_269	TCGA_SAMP_270	TCGA_SAMP_271	TCGA_SAMP_272	TCGA_SAMP_273	TCGA_SAMP_274	TCGA_SAMP_275	TCGA_SAMP_276	TCGA_SAMP_277	TCGA_SAMP_278	TCGA_SAMP_279	TCGA_SAMP_280	TCGA_SAMP_281	TCGA_SAMP_282	TCGA_SAMP_283	TCGA_SAMP_284	TCGA_SAMP_285	TCGA_SAMP_286	TCGA_SAMP_287	TCGA_SAMP_288	TCGA_SAMP_289	TCGA_SAMP_290	TCGA_SAMP_291	TCGA_SAMP_292	TCGA_SAMP_293	TCGA_SAMP_294	TCGA_SAMP_295	TCGA_SAMP_296	TCGA_SAMP_297	TCGA_SAMP_298	TCGA_SAMP_299	TCGA_SAMP_300	TCGA_SAMP_301	TCGA_SAMP_302	TCGA_SAMP_303	TCGA_SAMP_304	TCGA_SAMP_305	TCGA_SAMP_306	TCGA_SAMP_307	TCGA_SAMP_308	TCGA_SAMP_309	TCGA_SAMP_310	TCGA_SAMP_311	TCGA_SAMP_312	TCGA_SAMP_313	TCGA_SAMP_314	TCGA_SAMP_315	TCGA_SAMP_316	TCGA_SAMP_317	TCGA_SAMP_318	TCGA_SAMP_319	TCGA_SAMP_320	TCGA_SAMP_321	TCGA_SAMP_322	TCGA_SAMP_323	TCGA_SAMP_324	TCGA_SAMP_325	TCGA_SAMP_326	TCGA_SAMP_327	TCGA_SAMP_328	TCGA_SAMP_329	TCGA_SAMP_330	TCGA_SAMP_331	TCGA_SAMP_332	TCGA_SAMP_333	TCGA_SAMP_334	TCGA_SAMP_335	TCGA_SAMP_336	TCGA_SAMP_337	TCGA_SAMP_338	TCGA_SAMP_339	TCGA_SAMP_340	TCGA_SAMP_341	TCGA_SAMP_342	TCGA_SAMP_343	TCGA_SAMP_344	TCGA_SAMP_345	TCGA_SAMP_346	TCGA_SAMP_347	TCGA_SAMP_348	TCGA_SAMP_349	TCGA_SAMP_350	TCGA_SAMP_351	TCGA_SAMP_352	TCGA_SAMP_353	TCGA_SAMP_354	TCGA_SAMP_355	TCGA_SAMP_356	TCGA_SAMP_357	TCGA_SAMP_358	TCGA_SAMP_359	TCGA_SAMP_360	TCGA_SAMP_361	TCGA_SAMP_362	TCGA_SAMP_363	TCGA_SAMP_364	TCGA_SAMP_365	TCGA_SAMP_366	TCGA_SAMP_367	TCGA_SAMP_368	TCGA_SAMP_369	TCGA_SAMP_370	TCGA_SAMP_371	TCGA_SAMP_372	TCGA_SAMP_373	TCGA_SAMP_374	TCGA_SAMP_375	TCGA_SAMP_376	TCGA_SAMP_377	TCGA_SAMP_378	TCGA_SAMP_379	TCGA_SAMP_380	TCGA_SAMP_381	TCGA_SAMP_382	TCGA_SAMP_383	TCGA_SAMP_384	TCGA_SAMP_385	TCGA_SAMP_386	TCGA_SAMP_387	TCGA_SAMP_388	TCGA_SAMP_389	TCGA_SAMP_390	TCGA_SAMP_391	TCGA_SAMP_392	TCGA_SAMP_393	TCGA_SAMP_394	TCGA_SAMP_395	TCGA_SAMP_396	TCGA_SAMP_397	TCGA_SAMP_398	TCGA_SAMP_399	TCGA_SAMP_400
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