changeset 2:e8115b71edbd draft

planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit b9403b3ce2b7a41fa8ee1aa47909152de78cf641

--- a/fsd_beforevsafter.py	Sat May 12 04:51:34 2018 -0400
+++ b/fsd_beforevsafter.py	Tue May 15 13:50:02 2018 -0400
@@ -11,7 +11,7 @@
 # The program produces a plot which shows the distribution of family sizes of the DCS from the input files and
 # a CSV file with the data of the plot.
 
-# USAGE: python FSD before vs after_no_refF1.3_FINAL.py --inputFile_SSCS filenameSSCS --makeDCS filenameMakeDCS --afterTrimming filenameAfterTrimming -- alignedTags filenameTagsRefGenome 
+# USAGE: python FSD before vs after_no_refF1.3_FINAL.py --inputFile_SSCS filenameSSCS --inputName1 filenameSSCS --makeDCS filenameMakeDCS --afterTrimming filenameAfterTrimming -- alignedTags filenameTagsRefGenome 
 # --sep "characterWhichSeparatesCSVFile" --output_csv outptufile_name_csv --output_pdf outptufile_name_pdf
 
 
@@ -46,6 +46,7 @@
     parser = argparse.ArgumentParser(description='Analysis of read loss in duplex sequencing data')
     parser.add_argument('--inputFile_SSCS',
                         help='Tabular File with three columns: ab or ba, tag and family size.')
+    parser.add_argument('--inputName1')
     parser.add_argument('--makeDCS',
                         help='FASTA File with information about tag and family size in the header.')
     parser.add_argument('--afterTrimming',default=None,
@@ -65,6 +66,7 @@
     args = parser.parse_args(argv[1:])
 
     SSCS_file = args.inputFile_SSCS
+    SSCS_file_name = args.inputName1
     makeConsensus = args.makeDCS
     afterTrimming = args.afterTrimming
     ref_genome = args.alignedTags
@@ -80,8 +82,8 @@
         ### PLOT ###
         plt.rc('figure', figsize=(11.69, 8.27))  # A4 format
         plt.rcParams['axes.facecolor'] = "E0E0E0"  # grey background color
-        plt.rcParams['xtick.labelsize'] = 12
-        plt.rcParams['ytick.labelsize'] = 12
+        plt.rcParams['xtick.labelsize'] = 14
+        plt.rcParams['ytick.labelsize'] = 14
         plt.rcParams['patch.edgecolor'] = "black"
         fig = plt.figure()
         plt.subplots_adjust(bottom=0.3)
@@ -221,9 +223,9 @@
         plt.text(0.1, 0.02, legend4, size=11, transform=plt.gcf().transFigure)
 
         plt.legend(loc='upper right', fontsize=14, bbox_to_anchor=(0.9, 1), frameon=True)
-        plt.title("Family Size Distribution of Tags from various Steps of the Galaxy Pipeline", fontsize=14)
-        plt.xlabel("No. of Family Members", fontsize=12)
-        plt.ylabel("Absolute Frequency", fontsize=12)
+        plt.title("Family size distribution of tags from various steps of the Du Novo pipeline", fontsize=14)
+        plt.xlabel("Family size", fontsize=14)
+        plt.ylabel("Absolute Frequency", fontsize=14)
         plt.grid(b=True, which="major", color="#424242", linestyle=":")
         plt.margins(0.01, None)
 
@@ -231,7 +233,7 @@
         plt.close()
 
     # write information about plot into a csv file
-        output_file.write("Dataset:{}{}\n".format(sep, SSCS_file))
+        output_file.write("Dataset:{}{}\n".format(sep, SSCS_file_name))
         if ref_genome != str(None):
             output_file.write("{}AB{}BA\n".format(sep, sep))
             output_file.write("max. family size:{}{}{}{}\n".format(sep, max(quant_ab_ref), sep, max(quant_ba_ref)))

--- a/fsd_beforevsafter.xml	Sat May 12 04:51:34 2018 -0400
+++ b/fsd_beforevsafter.xml	Tue May 15 13:50:02 2018 -0400
@@ -1,5 +1,5 @@
 <?xml version="1.0" encoding="UTF-8"?>
-<tool id="fsd_beforevsafter" name="Duplex Sequencing Analysis:" version="0.0.2">
+<tool id="fsd_beforevsafter" name="Duplex Sequencing Analysis:" version="0.0.3">
     <requirements>
 		<!-- galaxy version 16.04 -->
         <requirement type="package" version="2.7">python</requirement>
@@ -9,7 +9,7 @@
     </requirements>
     <description>Family size distribution (FSD) of tags from various steps of the Du Novo pipeline</description>
     <command>
-        python2 $__tool_directory__/fsd_beforevsafter.py --inputFile_SSCS "$file1" --makeDCS "$makeDCS" --afterTrimming "$afterTrimming" --alignedTags "$alignedTags" --sep $separator --output_csv $output_csv --output_pdf $output_pdf
+        python2 $__tool_directory__/fsd_beforevsafter.py --inputFile_SSCS "$file1" --inputName1 "$file1.name" --makeDCS "$makeDCS" --afterTrimming "$afterTrimming" --alignedTags "$alignedTags" --sep $separator --output_pdf $output_pdf --output_csv $output_csv 
     </command>
     <inputs>
         <param name="file1" type="data" format="tabular" label="Dataset 1: input tags of whole dataset" optional="false" help="Input in tabular format with the family size, tags and the direction of the strand ('ab' or 'ba') for each family."/>
@@ -58,6 +58,7 @@
     
     
     
+	
     **Dataset 4 (optional):** Finally, a TXT file with the regions and all tags that were aligned to the reference genome can be given as input. This file can obtained from a different tool.
 
     +-----------+------------------------------+