annotate make_phylip.xml @ 39:e56023008e36 default tip

Changed revision of package_fisher_0_1_4 to be2fc454d121 Changed revision of package_matplotlib_1_2 to a03ee94316b5

<tool id="gd_make_phylip" name="Phylip" version="1.1.0" force_history_refresh="True">
  <description>: prepare data for phylogenetic analysis</description>

  <command interpreter="python">
    #set $zero_based = 1
    #set $gen_chrClmn = int($input.metadata.ref) - $zero_based
    #set $gen_posClmn = int($input.metadata.rPos) - $zero_based
    #set $gen_refClmn = int($input.metadata.pos) - $zero_based + 1
    #set $gen_altrClmn = int($input.metadata.pos) - $zero_based + 2
    make_phylip.py '--altrClmn=$gen_altrClmn' '--chrClmn=$gen_chrClmn' '--gd_indivs=$indivs_input' '--input=$input' '--input_type=$input.ext' '--output=$output1' '--output_id=$output1.id' '--posClmn=$gen_posClmn' '--refClmn=$gen_refClmn'
    #if $input_type.choice == '0'
      #set $cov_chrClmn = int($input_type.coverage_input.metadata.ref) - $zero_based
      #set $cov_posClmn = int($input_type.coverage_input.metadata.rPos) - $zero_based
      #set $cov_refClmn = int($input_type.coverage_input.metadata.pos) - $zero_based + 1
      #set $cov_altrClmn = int($input_type.coverage_input.metadata.pos) - $zero_based + 2
      '--altrClmnCvrg=$cov_altrClmn' '--chrClmnCvrg=$cov_chrClmn' '--cvrgTreshold=$input_type.coverage_threshold' '--gd_indivs_cover=$indivs_input' '--indvlsPrctTrshld=$input_type.indivs_threshold' '--inputCover=$input_type.coverage_input' '--inputCover_type=$input_type.coverage_input.ext' '--posClmnCvrg=$cov_posClmn' '--refClmnCvrg=$cov_refClmn'
    #else if $input_type.choice == '1'
      #set $fchrClmn = int($input_type.annotation_input.metadata.chromCol) - $zero_based
      #set $strandClmn = int($input_type.annotation_input.metadata.strandCol) - $zero_based
      #set $geneNameClmn = int($input_type.annotation_input.metadata.nameCol) - $zero_based
      #set $txStartClmn = int(str($input_type.tx_start_col)) - $zero_based
      #set $txEndClmn = int(str($input_type.tx_end_col)) - $zero_based
      #set $cdsStartClmn = int(str($input_type.cds_start_col)) - $zero_based
      #set $cdsEndClmn = int(str($input_type.cds_end_col)) - $zero_based
      #set $startExsClmn = int(str($input_type.exs_start_col)) - $zero_based
      #set $endExsClmn = int(str($input_type.exs_end_col)) - $zero_based
      '--cdsEndClmn=$cdsEndClmn' '--cdsStartClmn=$cdsStartClmn' '--endExsClmn=$endExsClmn' '--fchrClmn=$fchrClmn' '--geneNameClmn=$geneNameClmn' '--gene_info=$input_type.annotation_input' '--sequence=$input_type.fasta_input' '--startExsClmn=$startExsClmn' '--strandClmn=$strandClmn' '--txEndClmn=$txEndClmn' '--txStartClmn=$txStartClmn'
    #end if
  </command>

  <code file="make_phylip_hooks.py" />

  <inputs>
    <param name="input" type="data" format="gd_genotype,gd_snp" label="Genotype/SNP dataset">
      <validator type="metadata" check="scaffold" message="scaffold missing" />
      <validator type="metadata" check="pos" message="pos missing" />
    </param>
    <param name="indivs_input" type="data" format="gd_indivs" label="Individuals dataset" />
    <conditional name="input_type">
      <param name="choice" type="select" format="integer" label="Input type">
        <option value="0" selected="true">Coverage</option>
        <option value="1">Genes</option>
      </param>
      <when value="0">
        <param name="coverage_input" type="data" format="gd_genotype,gd_snp" label="Coverage dataset">
          <validator type="metadata" check="scaffold" message="scaffold missing" />
          <validator type="metadata" check="pos" message="pos missing" />
        </param>
        <param name="coverage_threshold" type="integer" min="1" value="1" label="Coverage threshold" />
        <param name="indivs_threshold" type="float" value="0.5" min="0.0" max="1.0" label="Individuals genotype percentage threshold" />
      </when>
      <when value="1">
        <param name="annotation_input" type="data" format="interval" label="Genes dataset">
          <validator type="metadata" check="chromCol" message="chromCol missing" />
          <validator type="metadata" check="strandCol" message="strandCol missing" />
          <validator type="metadata" check="nameCol" message="nameCol missing" />
        </param>
        <param name="tx_start_col" type="data_column" data_ref="input" label="Genes transcript start column" />
        <param name="tx_end_col" type="data_column" data_ref="input" label="Genes transcript end column" />
        <param name="cds_start_col" type="data_column" data_ref="input" label="Genes coding sequence start column" />
        <param name="cds_end_col" type="data_column" data_ref="input" label="Genes coding sequence end column" />
        <param name="exs_start_col" type="data_column" data_ref="input" label="Genes exon starts column" />
        <param name="exs_end_col" type="data_column" data_ref="input" label="Genes exon ends column" />
        <param name="fasta_input" type="data" format="fasta" label="FASTA dataset" />
      </when>
    </conditional>
  </inputs>

  <outputs>
    <data name="output1" format="txt" />
  </outputs>

  <help>
**What it does**

This tool creates phylip formatted files from two different input types:
coverage and genes.

If the coverage option is selected the inputs for the program are:

   1. a gd_indivs table
   2. a gd_genotype file with the coverage information for individuals in the gd_indivs table
   3. a gd_genotype file with the genotype information for individuals in the gd_indivs table
   4. a coverage threshold (optional)
   5. a percentage of individuals (threshold).

The program produces a phylip formatted file using the sequence in the
genotype file as a template.  In this sequence nucleotides for each
sequence that are below the coverage threshold, or the positions with
a percentage of individuals below the selected value are replaced by "N".

If the gene option is selected the inputs for the program are:

   1. a gd_indivs table
   2. a gene dataset table with a gene name in the first column
   3. the column with transcript start in the gene dataset table
   4. the column with transcript end in the gene dataset table
   5. the column with coding start in the gene dataset table
   6. the column with coding end in the gene dataset table
   7. the column with exon starts (comma-separated) in the gene dataset table
   8. the column with exon ends (comma-separated) in the gene dataset table
   9. a FASTA formatted file for all the genes of interest with their names as headers (NOTE: these names should be the same in the input gene dataset table).
   
The program produces as output one phylip formatted file for each gene
in the gene dataset table.

-----

**Example**

In a case were the option coverage is selected, for the inputs:

- gd_indivs::

   7       W_Java
   10      E_Java
   16      Pen_Ma
   ...

- Genotype table::

   chrM 15 T C -1 -1 2 -1 -1 2 -1 -1 -1 -1 -1 2 -1 -1 -1 -1 0 -1 -1
   chrM 18 G A -1 -1 0 -1 -1 0 -1 -1 -1 -1 -1 0 -1 -1 -1 -1 0 -1 -1
   chrM 20 C T -1 -1 0 -1 -1 2 -1 -1 -1 -1 -1 0 -1 -1 -1 -1 0 -1 -1
   ...

- Coverage table::

   chrM 0 G G 0 0 0 0 0 0 0 0 0 0 0 0 0
   chrM 1 T T 0 0 3 0 0 50 0 0 0 0 0 2 0
   chrM 2 T T 0 0 5 0 0 50 0 0 0 0 0 2 0
   ...

- Coverage threshold = 0

- Percentage of individuals = 0.0

- The output is::

   4 19 15428
   W_Java  GTTCATCATGTTCATCGAAT
   E_Java  GTTCATCATGTTCATCGAAC
   Pen_Ma  GTTCATCATGTTCATCGAAT

In a case were option genotype is selected with the inputs:

- Gene dataset table input::

   1 ENSLAFT00000017123 chrM + 1002 1061 1002 1061 1 1002, 1061, 0 ENSLAFG00000017122 cmpl incmpl 0, BTRC ENSLAFT00000017123 ENSLAFP00000014355
   1 ENSLAFT00000037164 chrM - 1058 1092 1062 1073 1 1062,1068 1065,1073 0 ENSLAFG00000007680 cmpl cmpl 0, MYOF ENSLAFT00000037164 ENSLAFP00000025175 26509
   1 ENSLAFT00000008925 chrM + 990 1000 990 1000 1 990, 1000, 0 ENSLAFG00000008924 incmpl incmpl 0, PRKG1 ENSLAFT00000008925 ENSLAFP00000007492
   ...

In this table:

   column with transcript start = 5
   column with transcript end = 6
   column with coding start = 7
   column with coding end = 8
   column with exon starts = 10
   column with exon ends = 11

- gd_indivs::

   7       W_Java
   10      E_Java
   16      Pen_Ma
   ...

- Genotype table::

   chrM 1005 T C -1 -1 2 -1 -1 2 -1 -1 -1 -1 -1 2 -1 -1 -1 -1 0 -1 -1
   chrM 1060 G A -1 -1 0 -1 -1 0 -1 -1 -1 -1 -1 0 -1 -1 -1 -1 0 -1 -1
   chrM 991 C T -1 -1 0 -1 -1 2 -1 -1 -1 -1 -1 0 -1 -1 -1 -1 0 -1 -1
   ...

The outputs are going to one file for each sequence in the input gene
dataset table (as long as they are included in the input FASTA file).
  </help>
</tool>