Mercurial > repos > mingchen0919 > aurora_deseq2_site
annotate 02_ma_plot.Rmd @ 1:32210899a3dd draft
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author | mingchen0919 |
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date | Sun, 30 Dec 2018 12:45:56 -0500 |
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1 --- |
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2 title: 'MA-plot' |
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3 output: |
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4 html_document: |
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5 highlight: pygments |
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6 --- |
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7 |
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8 ```{r setup, include=FALSE, warning=FALSE, message=FALSE} |
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9 knitr::opts_chunk$set(error = TRUE, echo = FALSE) |
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10 ``` |
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11 |
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12 |
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13 ```{r warning=FALSE} |
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14 log_fold_change = res$log2FoldChange |
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15 base_mean = res$baseMean |
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16 significant = res$padj |
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17 significant[significant < 0.1] = 'yes' |
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18 significant[significant != 'yes'] = 'no' |
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19 |
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20 maplot_df = data.frame(log_fold_change, base_mean, significant) |
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21 maplot_df = maplot_df[!is.na(maplot_df$significant), ] |
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22 p = ggplot(data = maplot_df) + |
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23 geom_point(mapping = aes(log(base_mean), log_fold_change, color = significant), |
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24 size = 0.5) + |
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25 scale_color_manual(name = 'Significant', |
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26 values = c('no' = 'black', 'yes' = 'red'), |
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27 labels = c('No', 'Yes')) + |
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28 xlab('Log base mean') + |
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29 ylab('Log fold change') + |
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30 theme_classic() |
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31 |
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32 plotly::ggplotly(p) |
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33 ``` |