changeset 0:dfe3cb3f3b39 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/DESeq_render.R	Mon Aug 07 18:10:13 2017 -0400
@@ -0,0 +1,113 @@
+##======= Handle arguments from command line ========
+# setup R error handline to go to stderr
+options(show.error.messages=FALSE,
+error=function(){
+    cat(geterrmessage(), file=stderr())
+    quit("no", 1, F)
+})
+
+# we need that to not crash galaxy with an UTF8 error on German LC settings.
+loc = Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
+
+# suppress warning
+options(warn = -1)
+
+options(stringsAsFactors=FALSE, useFancyQuotes=FALSE)
+args = commandArgs(trailingOnly=TRUE)
+
+suppressPackageStartupMessages({
+    library(getopt)
+    library(tools)
+})
+
+# column 1: the long flag name
+# column 2: the short flag alias. A SINGLE character string
+# column 3: argument mask
+#           0: no argument
+#           1: argument required
+#           2: argument is optional
+# column 4: date type to which the flag's argument shall be cast.
+#           possible values: logical, integer, double, complex, character.
+spec_list=list()
+
+##------- 1. input data ---------------------
+spec_list$COUNT_FILES = c('count_files', 'c', '1', 'character')
+spec_list$ECHO = c('echo', 'e', '1', 'character')
+spec_list$SAMPLE_TABLE = c('sample_table', 's', '1', 'character')
+spec_list$DESIGN_FORMULA = c('design_formula', 'p', '1', 'character')
+
+##--------2. output report and report site directory --------------
+spec_list$OUTPUT_HTML = c('deseq_html', 'o', '1', 'character')
+spec_list$OUTPUT_DIR = c('deseq_dir', 'd', '1', 'character')
+spec_list$WORKSPACE = c('deseq_workspace', 'w', '1', 'character')
+
+##--------3. Rmd templates sitting in the tool directory ----------
+
+spec_list$DESEQ_RMD = c('deseq_rmd', 'D', '1', 'character')
+
+
+
+##------------------------------------------------------------------
+
+spec = t(as.data.frame(spec_list))
+opt = getopt(spec)
+# arguments are accessed by long flag name (the first column in the spec matrix)
+#                        NOT by element name in the spec_list
+# example: opt$help, opt$expression_file
+##====== End of arguments handling ==========
+
+#------ Load libraries ---------
+library(rmarkdown)
+library(plyr)
+library(stringr)
+library(dplyr)
+library(highcharter)
+library(DT)
+library(reshape2)
+# library(Kmisc)
+library(plotly)
+library(formattable)
+library(htmltools)
+
+
+#----- 1. create the report directory ------------------------
+system(paste0('mkdir -p ', opt$deseq_dir))
+
+
+#----- 2. generate Rmd files with Rmd templates --------------
+#   a. templates without placeholder variables:
+#         copy templates from tool directory to the working directory.
+#   b. templates with placeholder variables:
+#         substitute variables with user input values and place them in the working directory.
+
+
+#----- 01 DESeq.Rmd -----------------------
+readLines(opt$deseq_rmd) %>%
+    (function(x) {
+        gsub('ECHO', opt$echo, x)
+    }) %>%
+    (function(x) {
+      gsub('DESEQ_WORKSPACE', opt$deseq_workspace, x)
+    }) %>%
+    (function(x) {
+      gsub('DESIGN_FORMULA', opt$design_formula, x)
+    }) %>%
+    (function(x) {
+        gsub('OUTPUT_DIR', opt$deseq_dir, x)
+    }) %>%
+    (function(x) {
+        fileConn = file('DESeq.Rmd')
+        writeLines(x, con=fileConn)
+        close(fileConn)
+    })
+
+
+#------ 3. render all Rmd files --------
+render('DESeq.Rmd', output_file = opt$deseq_html)
+
+
+#-------4. manipulate outputs -----------------------------
+# document file
+# file.copy('DESeq.html', opt$deseq_html, recursive = TRUE)
+
+