14
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1 ##======= Handle arguments from command line ========
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2 # setup R error handline to go to stderr
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3 options(show.error.messages = FALSE,
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4 error = function(){
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5 cat(geterrmessage(), file = stderr())
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6 quit("no", 1, F)
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7 })
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8
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9 # we need that to not crash galaxy with an UTF8 error on German LC settings.
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10 loc = Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
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11
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12 # suppress warning
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13 options(warn = - 1)
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14
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15 options(stringsAsFactors = FALSE, useFancyQuotes = FALSE)
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16 args = commandArgs(trailingOnly = TRUE)
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17
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18 suppressPackageStartupMessages({
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19 library(getopt)
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20 library(tools)
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21 })
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22
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23 # column 1: the long flag name
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24 # column 2: the short flag alias. A SINGLE character string
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25 # column 3: argument mask
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26 # 0: no argument
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27 # 1: argument required
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28 # 2: argument is optional
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29 # column 4: date type to which the flag's argument shall be cast.
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30 # possible values: logical, integer, double, complex, character.
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31 spec_list = list()
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32 spec_list$READS = c('reads', 'r', '1', 'character')
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33 spec_list$ECHO = c('echo', 'e', '1', 'character')
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34 spec_list$FASTQC_TPL = c('fastqc_tpl', 'p', 1, 'character')
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35 spec_list$REPORT = c('report', 'o', '1', 'character')
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36 spec_list$REPORT_OUTPUT_DIR = c('report_output_dir', 'd', '1', 'character')
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37
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38
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39 spec = t(as.data.frame(spec_list))
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40
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41 opt = getopt(spec)
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42 # arguments are accessed by long flag name (the first column in the spec matrix)
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43 # NOT by element name in the spec_list
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44 # example: opt$help, opt$expression_file
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45 ##====== End of arguments handling ==========
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46
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47
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48 mgsub = function(pattern, replacement, x) {
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49 if (length(pattern) != length(replacement)) {
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50 stop("pattern and replacement have to be the same in length")
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51 }
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52
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53 result = x
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54
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55 for (i in 1 : length(pattern)) {
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56 result = try(gsub(pattern[i], replacement[i], x = result))
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57 }
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58
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59 result
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60 }
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61
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62
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63 ##====== replace variables in tpl file ======
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64 p = c('READS',
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65 'ECHO',
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66 'FASTQC_TPL',
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67 'REPORT_OUTPUT_DIR',
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68 'REPORT')
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69 r = c(opt$reads,
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70 opt$echo,
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71 opt$fastqc_tpl,
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72 opt$report_output_dir,
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73 opt$report)
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74
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75 fastqc_report_tpl = mgsub(p, r, readLines(opt$fastqc_tpl))
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76
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77 ##====== write replaced text into Rmd file ===
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78 fileConn = file('fastqc_report.Rmd')
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79 writeLines(fastqc_report_tpl, con = fileConn)
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80 close(fileConn)
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81
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82 ##====== render Rmd files ====================
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83 rmarkdown::render('fastqc_report.Rmd')
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84 file.copy('fastqc_report.html', opt$report, recursive = TRUE)
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85 paste0('cp -r ./* ', opt$report_output_dir) %>%
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86 system()
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87
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