Mercurial > repos > peterjc > seq_filter_by_id
annotate tools/seq_filter_by_id/seq_filter_by_id.xml @ 3:44ab4c0f7683 draft
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author | peterjc |
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date | Fri, 11 Oct 2013 04:37:12 -0400 |
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children | 832c1fd57852 |
rev | line source |
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1 <tool id="seq_filter_by_id" name="Filter sequences by ID" version="0.0.6"> |
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2 <description>from a tabular file</description> |
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3 <requirements> |
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4 <requirement type="package" version="1.62">biopython</requirement> |
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5 <requirement type="python-module">Bio</requirement> |
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6 </requirements> |
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7 <version_command interpreter="python">seq_filter_by_id.py --version</version_command> |
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8 <command interpreter="python"> |
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9 seq_filter_by_id.py "$input_file" "$input_file.ext" |
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10 #if $output_choice_cond.output_choice=="both" |
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11 $output_pos $output_neg |
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12 #elif $output_choice_cond.output_choice=="pos" |
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13 $output_pos - |
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14 #elif $output_choice_cond.output_choice=="neg" |
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15 - $output_neg |
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16 #end if |
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17 ## TODO - Decide on best way to expose multiple ID files via the XML wrapper. |
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18 ## Single tabular file, can call the Python script with either UNION or INTERSECTION |
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19 UNION "$input_tabular" "$columns" |
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20 </command> |
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21 <stdio> |
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22 <!-- Anything other than zero is an error --> |
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23 <exit_code range="1:" /> |
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24 <exit_code range=":-1" /> |
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25 </stdio> |
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26 <inputs> |
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27 <param name="input_file" type="data" format="fasta,fastq,sff" label="Sequence file to filter on the identifiers" help="FASTA, FASTQ, or SFF format." /> |
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28 <param name="input_tabular" type="data" format="tabular" label="Tabular file containing sequence identifiers"/> |
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29 <param name="columns" type="data_column" data_ref="input_tabular" multiple="True" numerical="False" label="Column(s) containing sequence identifiers" help="Multi-select list - hold the appropriate key while clicking to select multiple columns"> |
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30 <validator type="no_options" message="Pick at least one column"/> |
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31 </param> |
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32 <conditional name="output_choice_cond"> |
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33 <param name="output_choice" type="select" label="Output positive matches, negative matches, or both?"> |
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34 <option value="both">Both positive matches (ID on list) and negative matches (ID not on list), as two files</option> |
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35 <option value="pos">Just positive matches (ID on list), as a single file</option> |
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36 <option value="neg">Just negative matches (ID not on list), as a single file</option> |
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37 </param> |
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38 <!-- Seems need these dummy entries here, compare this to indels/indel_sam2interval.xml --> |
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39 <when value="both" /> |
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40 <when value="pos" /> |
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41 <when value="neg" /> |
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42 </conditional> |
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43 </inputs> |
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44 <outputs> |
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45 <data name="output_pos" format="fasta" label="With matched ID"> |
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46 <!-- TODO - Replace this with format="input:input_fastq" if/when that works --> |
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47 <change_format> |
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48 <when input_dataset="input_file" attribute="extension" value="sff" format="sff" /> |
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49 <when input_dataset="input_file" attribute="extension" value="fastq" format="fastq" /> |
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50 <when input_dataset="input_file" attribute="extension" value="fastqsanger" format="fastqsanger" /> |
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51 <when input_dataset="input_file" attribute="extension" value="fastqsolexa" format="fastqsolexa" /> |
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52 <when input_dataset="input_file" attribute="extension" value="fastqillumina" format="fastqillumina" /> |
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53 <when input_dataset="input_file" attribute="extension" value="fastqcssanger" format="fastqcssanger" /> |
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54 </change_format> |
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55 <filter>output_choice_cond["output_choice"] != "neg"</filter> |
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56 </data> |
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57 <data name="output_neg" format="fasta" label="Without matched ID"> |
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58 <!-- TODO - Replace this with format="input:input_fastq" if/when that works --> |
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59 <change_format> |
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60 <when input_dataset="input_file" attribute="extension" value="sff" format="sff" /> |
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61 <when input_dataset="input_file" attribute="extension" value="fastq" format="fastq" /> |
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62 <when input_dataset="input_file" attribute="extension" value="fastqsanger" format="fastqsanger" /> |
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63 <when input_dataset="input_file" attribute="extension" value="fastqsolexa" format="fastqsolexa" /> |
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64 <when input_dataset="input_file" attribute="extension" value="fastqillumina" format="fastqillumina" /> |
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65 <when input_dataset="input_file" attribute="extension" value="fastqcssanger" format="fastqcssanger" /> |
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66 </change_format> |
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67 <filter>output_choice_cond["output_choice"] != "pos"</filter> |
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68 </data> |
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69 </outputs> |
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70 <tests> |
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71 <test> |
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72 <param name="input_file" value="k12_ten_proteins.fasta" ftype="fasta" /> |
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73 <param name="input_tabular" value="k12_hypothetical.tabular" ftype="tabular" /> |
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74 <param name="columns" value="1" /> |
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75 <param name="output_choice" value="pos" /> |
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76 <output name="output_pos" file="k12_hypothetical.fasta" ftype="fasta" /> |
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77 </test> |
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78 </tests> |
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79 <help> |
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80 **What it does** |
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81 |
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82 By default it divides a FASTA, FASTQ or Standard Flowgram Format (SFF) file in |
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83 two, those sequences with or without an ID present in the tabular file column(s) |
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84 specified. You can opt to have a single output file of just the matching records, |
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85 or just the non-matching ones. |
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86 |
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87 Note that the order of sequences in the original sequence file is preserved, as |
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88 is any Roche XML Manifest in an SFF file. Also, if any sequences share an |
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89 identifier (which would be very unusual in SFF files), duplicates are not removed. |
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90 |
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91 **Example Usage** |
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92 |
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93 You may have performed some kind of contamination search, for example running |
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94 BLASTN against a database of cloning vectors or bacteria, giving you a tabular |
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95 file containing read identifiers. You could use this tool to extract only the |
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96 reads without BLAST matches (i.e. those which do not match your contaminant |
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97 database). |
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98 |
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99 You may have a file of FASTA sequences which has been used with some analysis |
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100 tool giving tabular output, which has then been filtered on some criteria. |
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101 You can then use this tool to divide the original FASTA file into those entries |
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102 matching or not matching your criteria (those with or without their identifier |
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103 in the filtered tabular file). |
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104 |
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105 **References** |
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106 |
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107 If you use this Galaxy tool in work leading to a scientific publication please |
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108 cite the following papers: |
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109 |
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110 Peter J.A. Cock, Björn A. Grüning, Konrad Paszkiewicz and Leighton Pritchard (2013). |
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111 Galaxy tools and workflows for sequence analysis with applications |
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112 in molecular plant pathology. PeerJ 1:e167 |
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113 http://dx.doi.org/10.7717/peerj.167 |
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114 |
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115 This tool uses Biopython to read and write SFF files, so you may also wish to |
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116 cite the Biopython application note (and Galaxy too of course): |
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117 |
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118 Cock et al (2009). Biopython: freely available Python tools for computational |
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119 molecular biology and bioinformatics. Bioinformatics 25(11) 1422-3. |
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120 http://dx.doi.org/10.1093/bioinformatics/btp163 pmid:19304878. |
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121 |
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122 This tool is available to install into other Galaxy Instances via the Galaxy |
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123 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id |
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124 </help> |
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125 </tool> |