3
|
1
|
9
|
2 <tool id="pairScan" name="Scan paired-end reads for overlap" version="1.0.0">
|
|
3 <description> Scan paired-end reads for overlap </description>
|
3
|
4 <requirements>
|
|
5 <requirement type="package">python-levenshtein</requirement>
|
|
6 </requirements>
|
|
7 <command interpreter="python">
|
|
8 pairScan.py -f $fasta_input -o $min_overlap -m $max_mismatch -p $pass -b $bad -s $offset
|
|
9 </command>
|
|
10
|
|
11 <inputs>
|
9
|
12 <param format="fasta" type="data" name="fasta_input" label="Reads in FASTA format" />
|
|
13 <param name="min_overlap" type="integer" size="3" value="30" min="20" max="100" label="Minimum overlap length [nt] " />
|
3
|
14 <param name="max_mismatch" type="select" label="Maximum number of mismatches per 100 bp">
|
|
15 <option value="0">0</option>
|
|
16 <option value="1" selected="true">1</option>
|
|
17 <option value="2">2</option>
|
|
18 <option value="3">3</option>
|
|
19 <option value="4">4</option>
|
|
20 </param>
|
|
21 <param name="offset" type="select" label="Maximum offset">
|
|
22 <option value="0">0</option>
|
|
23 <option value="1">1</option>
|
|
24 <option value="2">2</option>
|
|
25 <option value="3">3</option>
|
|
26 <option value="4">4</option>
|
|
27 <option value="5" selected="true" >5</option>
|
|
28 <option value="6">6</option>
|
|
29 <option value="7">7</option>
|
|
30 <option value="8">8</option>
|
|
31 <option value="9">9</option>
|
|
32 <option value="10">10</option>
|
|
33 </param>
|
|
34
|
|
35
|
|
36
|
|
37 </inputs>
|
|
38
|
|
39
|
|
40 <outputs>
|
9
|
41 <data format="fasta" name="pass" label="Pairs with no overlap from dataset ${fasta_input.hid}"/>
|
|
42 <data format="fasta" name="bad" label="Overlaping pairs from dataset ${fasta_input.hid}"/>
|
3
|
43 </outputs>
|
|
44
|
|
45 <help>
|
|
46 **Scan paired reads for overlap**
|
|
47
|
|
48 </help>
|
|
49
|
|
50
|
|
51 </tool>
|