annotate repeat_annotate_custom.xml @ 2:3f8ae272f4f3 draft

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<tool id="repeat_annotate" name="RepeatExplorer Based Assembly Annotation" version="0.1.0" python_template_version="3.5">
    <requirements>
        <requirement type="package">repeatmasker</requirement>
        <requirement type="package">bioconductor-rtracklayer</requirement>
    </requirements>
    <command detect_errors="exit_code"><![CDATA[
        RepeatMasker -dir \$(pwd) '$input' -pa 32 -lib '$repeat_library' -xsmall -nolow -no_is -e ncbi -s
        &&
        ls -l * >&2 &&
        cp `basename $input`.out  $output2
        &&
        Rscript ${__tool_directory__}/clean_rm_output.R $output2 $output1

        ]]></command>
    <inputs>
        <param type="data" name="input" format="fasta" label="Genome/ Assembly to annotate" />
        <param type="data" name="repeat_library" format="fasta" label="RepeatExplorer based Library of Repetitive Sequences"
               help="custom database of repetitive sequences should be provided in fasta format. Sequence header should specify repeat class:
                     >sequence_id#classification_level1/classification_level2/..." />
    </inputs>
    <outputs>
        <data name="output1" format="gff3"  label="Repeat Annotation on ${on_string}, cleaned gff"/>
        <data name="output2" format="tabular" label="RepeatMasker on ${on_string}, original output" />
    </outputs>
    <help><![CDATA[
        This tools uses RepeatMasker to annotate repetitive sequences in the genome assemblie using custom library of repeats created from RepeatExplorer output.
        Library of repeats created from RepeatExplorer ouput are contigs and TAREAN consensus sequences in fasta format where sequence header containg information about classification of repeats as **>sequence_id#classification_level1/classification_level2/...**

        Classification in RepeatExplorer based library follows predetermined classification levels. User can however specify additional classification levels or ciompletelly custom classifications. Conflicts in annotations are resolved based on classification hierarchy.
    ]]></help>
</tool>