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1 #!/bin/sh
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2 #
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3 # Wrapper script to run RnaChipIntegrator as a Galaxy tool
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4 #
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5 # usage: sh rnachipintegrator_wrapper.sh [OPTIONS] <rnaseq_in> <chipseq_in> --output_xls <xls_out>
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6 #
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7 echo RnaChipIntegrator: analyse gene expression and ChIP data
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8 #
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9 # Collect command line options
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10 opts=
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11 output_xls=
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12 peaks_to_transcripts_out=
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13 zip_file=
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14 while [ ! -z "$1" ] ; do
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15 case $1 in
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16 --output_xls)
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17 shift; output_xls=$1
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18 ;;
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19 --summit_outputs)
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20 shift; peaks_to_transcripts_out=$1
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21 shift; tss_to_summits_out=$1
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22 ;;
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23 --peak_outputs)
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24 shift; transcripts_to_edges_out=$1
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25 shift; transcripts_to_edges_summary=$1
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26 shift; tss_to_edges_out=$1
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27 shift; tss_to_edges_summary=$1
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28 ;;
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29 --zip_file)
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30 shift; zip_file=$1
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31 ;;
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32 *)
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33 opts="$opts $1"
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34 ;;
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35 esac
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36 shift
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37 done
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38 #
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39 # Run RnaChipIntegrator
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40 # NB append stderr to stdout otherwise Galaxy job will fail
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41 # Direct output to a temporary directory
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42 outdir=`mktemp -d`
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43 base_name=galaxy
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44 cmd="RnaChipIntegrator.py --project=${outdir}/${base_name} $opts"
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45 echo $cmd
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46 $cmd 2>&1
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47 #
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48 # Check exit code
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49 exit_status=$?
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50 if [ "$exit_status" -ne "0" ] ; then
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51 echo RnaChipIntegrator exited with non-zero status >&2
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52 # Clean up and exit
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53 /bin/rm -rf $outdir
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54 exit $exit_status
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55 fi
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56 #
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57 # Deal with output files - XLS
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58 if [ -f "${outdir}/${base_name}.xls" ] ; then
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59 /bin/mv ${outdir}/${base_name}.xls $output_xls
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60 else
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61 echo No file ${outdir}/${base_name}.xls >&2
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62 # Clean up and exit
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63 /bin/rm -rf $outdir
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64 exit 1
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65 fi
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66 #
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67 # Zip file
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68 if [ ! -z "$zip_file" ] ; then
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69 for ext in "PeaksToTranscripts" "TSSToSummits" "TranscriptsToPeakEdges" "TranscriptsToPeakEdges_summary" "TSSToPeakEdges" "TSSToPeakEdges_summary" ; do
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70 txt_file=${outdir}/${base_name}_${ext}.txt
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71 if [ -f "$txt_file" ] ; then
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72 zip -j -g ${outdir}/archive.zip $txt_file
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73 fi
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74 done
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75 /bin/mv ${outdir}/archive.zip $zip_file
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76 fi
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77 #
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78 # Peaks to transcripts
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79 if [ ! -z "$peaks_to_transcripts_out" ] ; then
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80 outfile=${outdir}/${base_name}_PeaksToTranscripts.txt
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81 if [ -f "$outfile" ] ; then
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82 /bin/mv $outfile $peaks_to_transcripts_out
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83 else
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84 echo No file $outfile >&2
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85 fi
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86 fi
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87 #
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88 # TSS to summits
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89 if [ ! -z "$tss_to_summits_out" ] ; then
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90 outfile=${outdir}/${base_name}_TSSToSummits.txt
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91 if [ -f "$outfile" ] ; then
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92 /bin/mv $outfile $tss_to_summits_out
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93 else
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94 echo No file $outfile >&2
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95 fi
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96 fi
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97 #
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98 # Transcripts to Peak Edges
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99 if [ ! -z "$transcripts_to_edges_out" ] ; then
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100 outfile=${outdir}/${base_name}_TranscriptsToPeakEdges.txt
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101 if [ -f "$outfile" ] ; then
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102 /bin/mv $outfile $transcripts_to_edges_out
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103 else
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104 echo No file $outfile >&2
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105 fi
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106 fi
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107 if [ ! -z "$transcripts_to_edges_summary" ] ; then
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108 outfile=${outdir}/${base_name}_TranscriptsToPeakEdges_summary.txt
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109 if [ -f "$outfile" ] ; then
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110 /bin/mv $outfile $transcripts_to_edges_summary
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111 else
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112 echo No file $outfile >&2
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113 fi
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114 fi
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115 #
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116 # TSS to Peak Edges
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117 if [ ! -z "$tss_to_edges_out" ] ; then
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118 outfile=${outdir}/${base_name}_TSSToPeakEdges.txt
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119 if [ -f "$outfile" ] ; then
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120 /bin/mv $outfile $tss_to_edges_out
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121 else
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122 echo No file $outfile >&2
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123 fi
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124 fi
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125 if [ ! -z "$tss_to_edges_summary" ] ; then
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126 outfile=${outdir}/${base_name}_TSSToPeakEdges_summary.txt
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127 if [ -f "$outfile" ] ; then
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128 /bin/mv $outfile $tss_to_edges_summary
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129 else
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130 echo No file $outfile >&2
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131 fi
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132 fi
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133 #
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134 # Clean up
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135 /bin/rm -rf $outdir
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136 #
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137 # Done
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