Mercurial > repos > plus91-technologies > softsearch
diff README @ 29:79f80a903c8f draft default tip
Uploaded
author | plus91-technologies |
---|---|
date | Thu, 19 Jun 2014 02:43:07 -0400 |
parents | da3c9b9fb992 |
children |
line wrap: on
line diff
--- a/README Thu Jun 19 02:29:43 2014 -0400 +++ b/README Thu Jun 19 02:43:07 2014 -0400 @@ -1,10 +1,11 @@ SoftSearch: - --SoftSearch is a tools that uses soft-masked reads and discordant read pairs to identify structural variation(SV) in - illumina next-generation sequencing data. - --It is a Integration of Multiple Sequence Features to Identify Breakpoints of Structural Variations. - --Breakpoint is a place or time at which an interruption or change is made. - --SoftSearch requires only two inputs: a BAM file of aligned reads and fasta file of the genome to which the reads were aligned. + --SoftSearch is a tool that uses soft-masked reads and discordant read pairs to identify structural variants(SV) in + Illumina paired-end next-generation sequencing data. + --Using Soft Clipped reads in which one part of the read maps to the reference genome but the other part does not SoftSearch + can identify and call SVs including deletions, insertions, translocations, inversions, and tandem duplications + --SoftSearch requires two inputs: a BAM file of aligned reads and fasta file of the reference genome to which the reads + were aligned. Google Code Link: @@ -16,21 +17,21 @@ -Perl -Bedtools -SamTools - Must have an entry in environment variable + Must be accessible from the PATH environment variable ------------------------------------------------------------------------------------------------------------------ -How To Selecte fasta file from Database (Additional Configuration): +How To Select fasta file from Database (Additional Configuration): - --Select fasta_indexes.loc file for that go to shed_tools/toolshed.g2.bx.psu.edu/repos/plus91-technologies/softsearch/ + --A fasta_indexes.loc file must be located in shed_tools/toolshed.g2.bx.psu.edu/repos/plus91-technologies/softsearch/ 8578d978014c/softsearch/tool-data/ - --open the fasta_indexes.loc file and make changes like + --open the fasta_indexes.loc file and add an entry according to the example below <unique_build_id> <dbkey> <display_name> <file_base_path> eg: hg19full hg19 Human (Homo sapiens): hg19 Full /depot/data2/galaxy/hg19/sam/hg19full.fa - --open shed_tool_data_table_conf.xml file and add following. + --open shed_tool_data_table_conf.xml file and add the following <tables> <table name="fasta_indexes" comment_char="#">