annotate scripts/TEMP_Insertion.sh @ 21:9672fe07a232 draft default tip

planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
author mvdbeek
date Mon, 05 Dec 2016 09:58:47 -0500
parents e7d3dc3e0ec9
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
1 #!/bin/bash -x
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
2 # TEMP (Transposable Element Movement present in a Population)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
3 # 2013-06-14
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
4 # Jiali Zhuang(jiali.zhuang@umassmed.edu)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
5 # Zhiping Weng Lab
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
6 # Programs in Bioinformatics and Integrative Biology
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
7 # University of Massachusetts Medical School
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
8
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
9 #usage function
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
10 usage() {
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
11 echo -en "\e[1;36m"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
12 cat <<EOF
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
13
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
14 usage: $0 -i input_file.sorted.bam -s scripts_directory -o output_directory -r transposon_database.fa -t annotated_TEs.bed -m MISMATCH -f fragment_size -c CPUs -h
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
15
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
16 TEMP is a software package for detecting transposable elements (TEs)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
17 insertions and excisions from pooled high-throughput sequencing data.
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
18 Please send questions, suggestions and bug reports to:
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
19 jiali.zhuang@umassmed.edu
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
20
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
21 Options:
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
22 -i Input file in bam format with full path. Please sort and index the file before calling this program.
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
23 Sorting and indexing can be done by 'samtools sort' and 'samtools index'
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
24 -s Directory where all the scripts are
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
25 -o Path to output directory. Default is current directory
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
26 -r Transposon sequence database in fasta format with full path
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
27 -t Annotated TEs in BED6 format with full path. Detected insertions that overlap with annoated TEs will be filtered.
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
28 -u TE families annotations. If supplied detected insertions overlap with annotated TE of the same family will be filtered. Only use with -t.
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
29 -m Number of mismatch allowed when mapping to TE concensus sequences. Default is 3
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
30 -x The minimum score difference between the best hit and the second best hit for considering a read as uniquely mapped. For BWA mem.
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
31 -f An integer specifying the length of the fragments (inserts) of the library. Default is 500
12
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
32 -c An integer specifying the number of CPUs used. Default is 8
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
33 -h Show help message
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
34
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
35 EOF
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
36 echo -en "\e[0m"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
37 }
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
38
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
39 # taking options
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
40 while getopts "hi:c:f:m:o:r:s:t:u:x:" OPTION
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
41 do
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
42 case $OPTION in
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
43 h)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
44 usage && exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
45 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
46 i)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
47 BAM=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
48 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
49 f)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
50 INSERT=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
51 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
52 m)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
53 MM=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
54 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
55 o)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
56 OUTDIR=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
57 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
58 c)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
59 CPU=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
60 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
61 s)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
62 BINDIR=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
63 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
64 r)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
65 TESEQ=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
66 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
67 t)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
68 ANNO=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
69 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
70 u)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
71 FAMI=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
72 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
73 x)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
74 SCORE=$OPTARG
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
75 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
76 ?)
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
77 usage && exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
78 ;;
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
79 esac
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
80 done
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
81
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
82 if [[ -z $BAM ]] || [[ -z $BINDIR ]] || [[ -z $TESEQ ]]
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
83 then
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
84 usage && exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
85 fi
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
86 [ ! -z "${CPU##*[!0-9]*}" ] || CPU=8
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
87 [ ! -z "${INSERT##*[!0-9]*}" ] || INSERT=500
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
88 [ ! -z "${MM##*[!0-9]*}" ] || MM=3
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
89 [ ! -z "${SCORE##*[!0-9]*}" ] || SCORE=0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
90 [ ! -z $OUTDIR ] || OUTDIR=$PWD
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
91
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
92 mkdir -p "${OUTDIR}" || echo -e "\e[1;31mWarning: Cannot create directory ${OUTDIR}. Using the direcory of input fastq file\e[0m"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
93 cd ${OUTDIR} || echo -e "\e[1;31mError: Cannot access directory ${OUTDIR}... Exiting...\e[0m" || exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
94 touch ${OUTDIR}/.writting_permission && rm -rf ${OUTDIR}/.writting_permission || echo -e "\e[1;31mError: Cannot write in directory ${OUTDIR}... Exiting...\e[0m" || exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
95
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
96 function checkExist {
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
97 echo -ne "\e[1;32m\"${1}\" is using: \e[0m" && which "$1"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
98 [[ $? != 0 ]] && echo -e "\e[1;36mError: cannot find software/function ${1}! Please make sure that you have installed the pipeline correctly.\nExiting...\e[0m" && exit 1
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
99 }
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
100 echo -e "\e[1;35mTesting required softwares/scripts:\e[0m"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
101 checkExist "echo"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
102 checkExist "rm"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
103 checkExist "mkdir"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
104 checkExist "date"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
105 checkExist "mv"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
106 checkExist "sort"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
107 checkExist "touch"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
108 checkExist "awk"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
109 checkExist "grep"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
110 checkExist "bwa"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
111 checkExist "samtools"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
112 echo -e "\e[1;35mDone with testing required softwares/scripts, starting pipeline...\e[0m"
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
113
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
114 cp $TESEQ ./
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
115 name=`basename $BAM`
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
116 te=`basename $TESEQ`
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
117 i=${name/.sorted.bam/}
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
118 echo $name
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
119 echo $i
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
120 if [[ ! -s $name ]]
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
121 then
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
122 cp $BAM ./
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
123 fi
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
124 if [[ ! -s $name.bai ]]
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
125 then cp $BAM.bai ./
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
126 fi
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
127
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
128 # Get the mate seq of the uniq-unpaired reads
21
9672fe07a232 planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents: 17
diff changeset
129 samtools view -F 0x2 $name > $i.unpair.sam
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
130 if [[ $SCORE -eq 0 ]]
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
131 then
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
132 perl $BINDIR/pickUniqPairFastq.pl $i.unpair.sam $i.unpair.uniq
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
133 perl $BINDIR/pickUniqPos.pl $i.unpair.sam > $i.unpair.uniq.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
134 else
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
135 perl $BINDIR/pickUniqPairFastq_MEM.pl $i.unpair.sam $i.unpair.uniq $SCORE
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
136 perl $BINDIR/pickUniqPos_MEM.pl $i.unpair.sam $SCORE > $i.unpair.uniq.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
137 fi
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
138
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
139 # Map to transposons
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
140 bwa index -a is $te
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
141 bwa aln -t $CPU -n $MM -l 100 -R 1000 $te $i.unpair.uniq.1.fastq > $i.unpair.uniq.1.sai
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
142 bwa aln -t $CPU -n $MM -l 100 -R 1000 $te $i.unpair.uniq.2.fastq > $i.unpair.uniq.2.sai
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
143 bwa sampe -P $te $i.unpair.uniq.1.sai $i.unpair.uniq.2.sai $i.unpair.uniq.1.fastq $i.unpair.uniq.2.fastq > $i.unpair.uniq.transposons.sam
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
144
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
145
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
146 #Summary
21
9672fe07a232 planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents: 17
diff changeset
147 samtools view -hSF 0x2 $i.unpair.uniq.transposons.sam > $i.unpair.uniq.transposons.unpair.sam
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
148 perl $BINDIR/pickUniqMate.pl $i.unpair.uniq.transposons.unpair.sam $i.unpair.uniq.bed > $i.unpair.uniq.transposons.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
149 cp $i.unpair.uniq.transposons.bed $i.unpair.uniq.transposons.filtered.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
150
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
151
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
152 #Prepare for insertion breakpoints identification
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
153 awk -F "\t" -v sample=$i '{OFS="\t"; print $1,$2,$3,sample,$5,$6}' $i.unpair.uniq.transposons.filtered.bed >> tmp
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
154 perl $BINDIR/mergeTagsWithoutGap.pl tmp > $i.uniq.transposons.filtered.woGap.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
155 perl $BINDIR/mergeTagsWithGap.pl $i.uniq.transposons.filtered.woGap.bed $INSERT > $i.uniq.transposons.filtered.wGap.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
156 rm tmp
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
157 perl $BINDIR/get_class.pl $i.uniq.transposons.filtered.wGap.bed $i > $i.uniq.transposons.filtered.wGap.class.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
158 perl $BINDIR/make.bp.bed.pl $i.uniq.transposons.filtered.wGap.class.bed $ANNO $FAMI
17
e7d3dc3e0ec9 planemo upload for repository https://github.com/portiahollyoak/Tools commit 22724a75342e3097cd0976e4bfcfe7a19308ac4f-dirty
portiahollyoak
parents: 12
diff changeset
159 echo "got FAMI"
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
160
12
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
161 rm $i.unpair.sam $i.unpair.uniq.bed $i.unpair.uniq.?.fastq $i.unpair.uniq.?.sai
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
162 rm $i.unpair.uniq.transposons.sam $i.unpair.uniq.transposons.unpair.sam $i.uniq.transposons.filtered.woGap.bed $i.uniq.transposons.filtered.wGap.bed
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
163
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
164
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
165 #Detect insertion breakpoints using soft-clipping information
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
166 perl $BINDIR/pickClippedFastq.pl $i $te
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
167 perl $BINDIR/refine_breakpoint.in.pl
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
168
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
169
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
170 #Estimate insertion frequencies
12
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
171 #if [[ $SCORE -eq 0 ]]
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
172 #then
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
173 perl $BINDIR/pickOverlapPair.in.pl $i.insertion.refined.bp $INSERT > $i.insertion.refined.bp.summary
12
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
174 #else
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
175 # perl $BINDIR/pickOverlapPair.ex_MEM.pl $i.insertion.refined.bp $INSERT $SCORE > $i.insertion.refined.bp.summary
ca36262102d8 planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
portiahollyoak
parents: 0
diff changeset
176 #fi
0
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
177
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
178 ################################
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
179 ##End of processing insertions##
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
180 ################################
28d1a6f8143f planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff changeset
181