annotate qiime2__rescript__orient_reads.xml @ 2:4d0fbac4fc34 draft default tip

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date Sat, 01 Nov 2025 17:29:46 +0000
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1 <?xml version='1.0' encoding='utf-8'?>
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2 <!--
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3 Copyright (c) 2025, QIIME 2 development team.
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5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause)
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6 -->
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7 <!--
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8 This tool was automatically generated by:
2
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9 q2galaxy (version: 2025.10.0)
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10 for:
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11 qiime2 (version: 2025.10.0)
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12 -->
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13 <tool name="qiime2 rescript orient-reads" id="qiime2__rescript__orient_reads" version="2025.10.0+q2galaxy.2025.10.0" profile="22.05" license="BSD-3-Clause">
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14 <description>Orient FASTQ reads against reference.</description>
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15 <xrefs>
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16 <xref type="bio.tools">qiime2</xref>
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17 </xrefs>
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18 <requirements>
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19 <container type="docker">quay.io/qiime2/amplicon:2025.10</container>
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20 </requirements>
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21 <version_command>q2galaxy version rescript</version_command>
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22 <command detect_errors="exit_code">q2galaxy run rescript orient_reads '$inputs'</command>
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23 <configfiles>
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24 <inputs name="inputs" data_style="staging_path_and_source_path"/>
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25 </configfiles>
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26 <inputs>
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27 <param name="sequences" type="data" format="qza" label="sequences: SampleData[PairedEndSequencesWithQuality¹ | JoinedSequencesWithQuality²]" help="[required] Sequence reads to be oriented.">
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28 <options options_filter_attribute="metadata.semantic_type">
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29 <filter type="add_value" value="SampleData[JoinedSequencesWithQuality]"/>
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30 <filter type="add_value" value="SampleData[PairedEndSequencesWithQuality]"/>
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31 </options>
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32 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[JoinedSequencesWithQuality]', 'SampleData[PairedEndSequencesWithQuality]']</validator>
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33 </param>
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34 <param name="reference_sequences" type="data" format="qza" label="reference_sequences: FeatureData[Sequence]" help="[required] Reference sequences to orient against.">
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35 <options options_filter_attribute="metadata.semantic_type">
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36 <filter type="add_value" value="FeatureData[Sequence]"/>
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37 </options>
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38 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]']</validator>
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39 </param>
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40 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options">
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41 <param name="dbmask" type="select" label="dbmask: Str % Choices('none', 'dust', 'soft')" display="radio">
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42 <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option>
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43 <option value="none">none</option>
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44 <option value="dust">dust</option>
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45 <option value="soft">soft</option>
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46 </param>
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47 </section>
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48 </inputs>
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49 <outputs>
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50 <data name="oriented_reads" format="qza" label="${tool.name} on ${on_string}: oriented_reads.qza" from_work_dir="oriented_reads.qza"/>
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51 <data name="unmatched_reads" format="qza" label="${tool.name} on ${on_string}: unmatched_reads.qza" from_work_dir="unmatched_reads.qza"/>
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52 </outputs>
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53 <tests/>
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54 <help>
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55 QIIME 2: rescript orient-reads
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56 ==============================
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57 Orient FASTQ reads against reference.
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58
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59
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60 Outputs:
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61 --------
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62 :oriented_reads.qza: Oriented reads.
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63 :unmatched_reads.qza: Reads that fail to match at least one reference sequence in either + or - orientation.
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64
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65 |
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66
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67 Description:
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68 ------------
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69 Orient input reads (FASTQ) by comparison against a set of reference sequences using VSEARCH. This action is useful for orienting reads that are in mixed orientations prior to denoising or clustering.
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70
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71
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72 |
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73
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74 </help>
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75 <citations>
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76 <citation type="doi">10.7717/peerj.2584</citation>
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77 <citation type="doi">10.1371/journal.pcbi.1009581</citation>
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78 <citation type="doi">10.1038/s41587-019-0209-9</citation>
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79 </citations>
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80 </tool>