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date | Wed, 30 Oct 2024 19:56:51 +0000 |
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1 <?xml version='1.0' encoding='utf-8'?> | |
2 <!-- | |
3 Copyright (c) 2024, QIIME 2 development team. | |
4 | |
5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) | |
6 --> | |
7 <!-- | |
8 This tool was automatically generated by: | |
9 q2galaxy (version: 2024.10.0) | |
10 for: | |
11 qiime2 (version: 2024.10.1) | |
12 --> | |
13 <tool name="qiime2 stats alpha-group-significance" id="qiime2__stats__alpha_group_significance" version="0+unknown-q2galaxy.2024.10.0" profile="22.05" license="BSD-3-Clause"> | |
14 <description>Alpha group significance test and plot</description> | |
15 <xrefs> | |
16 <xref type="bio.tools">qiime2</xref> | |
17 </xrefs> | |
18 <requirements> | |
19 <container type="docker">quay.io/qiime2/amplicon:2024.10</container> | |
20 </requirements> | |
21 <version_command>q2galaxy version stats</version_command> | |
22 <command detect_errors="exit_code">q2galaxy run stats alpha_group_significance '$inputs'</command> | |
23 <configfiles> | |
24 <inputs name="inputs" data_style="staging_path_and_source_path"/> | |
25 </configfiles> | |
26 <inputs> | |
27 <param name="alpha_diversity" type="data" format="qza" label="alpha_diversity: SampleData[AlphaDiversity]" help="[required] Alpha diversity which will become the "measure""> | |
28 <options options_filter_attribute="metadata.semantic_type"> | |
29 <filter type="add_value" value="SampleData[AlphaDiversity]"/> | |
30 </options> | |
31 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[AlphaDiversity]']</validator> | |
32 </param> | |
33 <repeat name="metadata" min="1" help="[required] Sample metadata to use" title="metadata: Metadata"> | |
34 <conditional name="__q2galaxy__GUI__conditional__metadata__"> | |
35 <param name="type" type="select" label="metadata: Metadata"> | |
36 <option value="tsv" selected="true">Metadata from TSV</option> | |
37 <option value="qza">Metadata from Artifact</option> | |
38 </param> | |
39 <when value="tsv"> | |
40 <param name="source" type="data" format="tabular,qiime2.tabular" label="Metadata Source"/> | |
41 </when> | |
42 <when value="qza"> | |
43 <param name="source" type="data" format="qza" label="Metadata Source"/> | |
44 </when> | |
45 </conditional> | |
46 </repeat> | |
47 <repeat name="columns" min="1" help="[required] Columns to include as group information" title="columns: List[Str]"> | |
48 <param name="element" type="text" label="element: Str" help="[required]"> | |
49 <sanitizer> | |
50 <valid initial="string.printable"/> | |
51 </sanitizer> | |
52 <validator type="expression" message="Please verify this parameter.">value is not None and len(value) > 0</validator> | |
53 </param> | |
54 </repeat> | |
55 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> | |
56 <conditional name="__q2galaxy__GUI__conditional__subject__"> | |
57 <param name="__q2galaxy__GUI__select__" type="select" label="subject: Str % Choices('')¹'³ | Str²'⁴" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> | |
58 <option value="" selected="true"> (Str)</option> | |
59 <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> | |
60 <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> | |
61 </param> | |
62 <when value=""> | |
63 <param name="subject" type="hidden" value=""/> | |
64 </when> | |
65 <when value="__q2galaxy__::control::Str"> | |
66 <param name="subject" type="text" value="" label="subject: Str" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> | |
67 <sanitizer> | |
68 <valid initial="string.printable"/> | |
69 </sanitizer> | |
70 </param> | |
71 </when> | |
72 <when value="__q2galaxy__::control::Str"> | |
73 <param name="subject" type="text" value="" label="subject: Str" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> | |
74 <sanitizer> | |
75 <valid initial="string.printable"/> | |
76 </sanitizer> | |
77 </param> | |
78 </when> | |
79 </conditional> | |
80 <conditional name="__q2galaxy__GUI__conditional__timepoint__"> | |
81 <param name="__q2galaxy__GUI__select__" type="select" label="timepoint: Str % Choices('')¹'² | Str³'⁴" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> | |
82 <option value="" selected="true"> (Str)</option> | |
83 <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> | |
84 <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> | |
85 </param> | |
86 <when value=""> | |
87 <param name="timepoint" type="hidden" value=""/> | |
88 </when> | |
89 <when value="__q2galaxy__::control::Str"> | |
90 <param name="timepoint" type="text" value="" label="timepoint: Str" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> | |
91 <sanitizer> | |
92 <valid initial="string.printable"/> | |
93 </sanitizer> | |
94 </param> | |
95 </when> | |
96 <when value="__q2galaxy__::control::Str"> | |
97 <param name="timepoint" type="text" value="" label="timepoint: Str" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> | |
98 <sanitizer> | |
99 <valid initial="string.printable"/> | |
100 </sanitizer> | |
101 </param> | |
102 </when> | |
103 </conditional> | |
104 </section> | |
105 </inputs> | |
106 <outputs> | |
107 <data name="distribution" format="qza" label="${tool.name} on ${on_string}: distribution.qza" from_work_dir="distribution.qza"/> | |
108 <data name="stats" format="qza" label="${tool.name} on ${on_string}: stats.qza" from_work_dir="stats.qza"/> | |
109 <data name="raincloud" format="qzv" label="${tool.name} on ${on_string}: raincloud.qzv" from_work_dir="raincloud.qzv"/> | |
110 </outputs> | |
111 <tests> | |
112 <test> | |
113 <param name="alpha_diversity" value="alpha_group_significance.test0.alpha-div-faith-pd.qza" ftype="qza"/> | |
114 <repeat name="metadata"> | |
115 <conditional name="__q2galaxy__GUI__conditional__metadata__"> | |
116 <param name="type" value="tsv"/> | |
117 <param name="source" value="alpha_group_significance.test0.metadata.tsv" ftype="qiime2.tabular"/> | |
118 </conditional> | |
119 </repeat> | |
120 <repeat name="columns"> | |
121 <param name="element" value="genotype"/> | |
122 </repeat> | |
123 <repeat name="columns"> | |
124 <param name="element" value="donor_status"/> | |
125 </repeat> | |
126 <conditional name="__q2galaxy__GUI__conditional__subject__"> | |
127 <param name="__q2galaxy__GUI__select__" value="__q2galaxy__::control::Str"/> | |
128 <param name="subject" value="mouse_id"/> | |
129 </conditional> | |
130 <conditional name="__q2galaxy__GUI__conditional__timepoint__"> | |
131 <param name="__q2galaxy__GUI__select__" value="__q2galaxy__::control::Str"/> | |
132 <param name="timepoint" value="days_post_transplant"/> | |
133 </conditional> | |
134 <output name="distribution" ftype="qza"> | |
135 <assert_contents> | |
136 <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> | |
137 <has_line_matching expression="type: Dist1D\[NestedOrdered,\ Matched\]"/> | |
138 </has_archive_member> | |
139 </assert_contents> | |
140 </output> | |
141 <output name="stats" ftype="qza"/> | |
142 <output name="raincloud" ftype="qzv"/> | |
143 <param name="alpha_diversity" value="alpha_group_significance.test0.alpha-div-faith-pd.qza" ftype="qza"/> | |
144 <repeat name="metadata"> | |
145 <conditional name="__q2galaxy__GUI__conditional__metadata__"> | |
146 <param name="type" value="tsv"/> | |
147 <param name="source" value="alpha_group_significance.test0.metadata.tsv" ftype="qiime2.tabular"/> | |
148 </conditional> | |
149 </repeat> | |
150 <repeat name="columns"> | |
151 <param name="element" value="genotype"/> | |
152 </repeat> | |
153 <repeat name="columns"> | |
154 <param name="element" value="donor_status"/> | |
155 </repeat> | |
156 <output name="distribution" ftype="qza"> | |
157 <assert_contents> | |
158 <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> | |
159 <has_line_matching expression="type: Dist1D\[Multi,\ Independent\]"/> | |
160 </has_archive_member> | |
161 </assert_contents> | |
162 </output> | |
163 <output name="stats" ftype="qza"/> | |
164 <output name="raincloud" ftype="qzv"/> | |
165 </test> | |
166 </tests> | |
167 <help> | |
168 QIIME 2: stats alpha-group-significance | |
169 ======================================= | |
170 Alpha group significance test and plot | |
171 | |
172 | |
173 Outputs: | |
174 -------- | |
175 :distribution.qza: Dist1D generated by metadata and alpha diversity. | |
176 :stats.qza: A stats table of the per-group/timepoint results | |
177 :raincloud.qzv: A visualization of the distribution and statistics | |
178 | |
179 | | |
180 | |
181 Description: | |
182 ------------ | |
183 Will select between Wilcoxon SRT and Mann-Whitney U depending on the presence of repeated measures. | |
184 | |
185 Examples: | |
186 --------- | |
187 | |
188 alpha_group_significance_faith_pd | |
189 ********************************* | |
190 Using the ``qiime2 stats alpha-group-significance`` tool: | |
191 #. Set *"alpha_diversity"* to ``#: alpha-div-faith-pd.qza`` | |
192 #. For *"metadata"*: | |
193 | |
194 - Perform the following steps. | |
195 | |
196 #. Leave as ``Metadata from TSV`` | |
197 #. Set *"Metadata Source"* to ``metadata.tsv`` | |
198 | |
199 | |
200 #. For *"columns"*, use the ``+ columns`` button to add the corresponding values: | |
201 | |
202 #. Add *"element"* set to ``genotype`` | |
203 #. Add *"element"* set to ``donor_status`` | |
204 | |
205 #. Expand the ``additional options`` section | |
206 | |
207 #. Set *"subject"* to ``mouse_id`` | |
208 #. Set *"timepoint"* to ``days_post_transplant`` | |
209 | |
210 #. Press the ``Execute`` button. | |
211 | |
212 Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: | |
213 (Renaming is optional, but it will make any subsequent steps easier to complete.) | |
214 | |
215 .. list-table:: | |
216 :align: left | |
217 :header-rows: 1 | |
218 | |
219 * - History Name | |
220 - *"Name"* to set (be sure to press ``Save``) | |
221 * - ``#: qiime2 stats alpha-group-significance [...] : distribution.qza`` | |
222 - ``dist.qza`` | |
223 * - ``#: qiime2 stats alpha-group-significance [...] : stats.qza`` | |
224 - ``stats.qza`` | |
225 * - ``#: qiime2 stats alpha-group-significance [...] : raincloud.qzv`` | |
226 - ``raincloud.qzv`` | |
227 | |
228 Using the ``qiime2 stats alpha-group-significance`` tool: | |
229 #. Set *"alpha_diversity"* to ``#: alpha-div-faith-pd.qza`` | |
230 #. For *"metadata"*: | |
231 | |
232 - Perform the following steps. | |
233 | |
234 #. Leave as ``Metadata from TSV`` | |
235 #. Set *"Metadata Source"* to ``metadata.tsv`` | |
236 | |
237 | |
238 #. For *"columns"*, use the ``+ columns`` button to add the corresponding values: | |
239 | |
240 #. Add *"element"* set to ``genotype`` | |
241 #. Add *"element"* set to ``donor_status`` | |
242 | |
243 #. Press the ``Execute`` button. | |
244 | |
245 Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: | |
246 (Renaming is optional, but it will make any subsequent steps easier to complete.) | |
247 | |
248 .. list-table:: | |
249 :align: left | |
250 :header-rows: 1 | |
251 | |
252 * - History Name | |
253 - *"Name"* to set (be sure to press ``Save``) | |
254 * - ``#: qiime2 stats alpha-group-significance [...] : distribution.qza`` | |
255 - ``dist2.qza`` | |
256 * - ``#: qiime2 stats alpha-group-significance [...] : stats.qza`` | |
257 - ``stats2.qza`` | |
258 * - ``#: qiime2 stats alpha-group-significance [...] : raincloud.qzv`` | |
259 - ``raincloud2.qzv`` | |
260 | |
261 | |
262 | | |
263 | |
264 </help> | |
265 <citations> | |
266 <citation type="doi">10.1038/s41587-019-0209-9</citation> | |
267 </citations> | |
268 </tool> |