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date | Wed, 30 Oct 2024 19:56:51 +0000 |
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<?xml version='1.0' encoding='utf-8'?> <!-- Copyright (c) 2024, QIIME 2 development team. Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) --> <!-- This tool was automatically generated by: q2galaxy (version: 2024.10.0) for: qiime2 (version: 2024.10.1) --> <tool name="qiime2 stats alpha-group-significance" id="qiime2__stats__alpha_group_significance" version="0+unknown-q2galaxy.2024.10.0" profile="22.05" license="BSD-3-Clause"> <description>Alpha group significance test and plot</description> <xrefs> <xref type="bio.tools">qiime2</xref> </xrefs> <requirements> <container type="docker">quay.io/qiime2/amplicon:2024.10</container> </requirements> <version_command>q2galaxy version stats</version_command> <command detect_errors="exit_code">q2galaxy run stats alpha_group_significance '$inputs'</command> <configfiles> <inputs name="inputs" data_style="staging_path_and_source_path"/> </configfiles> <inputs> <param name="alpha_diversity" type="data" format="qza" label="alpha_diversity: SampleData[AlphaDiversity]" help="[required] Alpha diversity which will become the "measure""> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="SampleData[AlphaDiversity]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[AlphaDiversity]']</validator> </param> <repeat name="metadata" min="1" help="[required] Sample metadata to use" title="metadata: Metadata"> <conditional name="__q2galaxy__GUI__conditional__metadata__"> <param name="type" type="select" label="metadata: Metadata"> <option value="tsv" selected="true">Metadata from TSV</option> <option value="qza">Metadata from Artifact</option> </param> <when value="tsv"> <param name="source" type="data" format="tabular,qiime2.tabular" label="Metadata Source"/> </when> <when value="qza"> <param name="source" type="data" format="qza" label="Metadata Source"/> </when> </conditional> </repeat> <repeat name="columns" min="1" help="[required] Columns to include as group information" title="columns: List[Str]"> <param name="element" type="text" label="element: Str" help="[required]"> <sanitizer> <valid initial="string.printable"/> </sanitizer> <validator type="expression" message="Please verify this parameter.">value is not None and len(value) > 0</validator> </param> </repeat> <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> <conditional name="__q2galaxy__GUI__conditional__subject__"> <param name="__q2galaxy__GUI__select__" type="select" label="subject: Str % Choices('')¹'³ | Str²'⁴" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> <option value="" selected="true"> (Str)</option> <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> </param> <when value=""> <param name="subject" type="hidden" value=""/> </when> <when value="__q2galaxy__::control::Str"> <param name="subject" type="text" value="" label="subject: Str" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> <when value="__q2galaxy__::control::Str"> <param name="subject" type="text" value="" label="subject: Str" help="[default: ''] If provided, will cause the results to be matched for repeated measures."> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> </conditional> <conditional name="__q2galaxy__GUI__conditional__timepoint__"> <param name="__q2galaxy__GUI__select__" type="select" label="timepoint: Str % Choices('')¹'² | Str³'⁴" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> <option value="" selected="true"> (Str)</option> <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> <option value="__q2galaxy__::control::Str" selected="true">Provide a value (Str)</option> </param> <when value=""> <param name="timepoint" type="hidden" value=""/> </when> <when value="__q2galaxy__::control::Str"> <param name="timepoint" type="text" value="" label="timepoint: Str" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> <when value="__q2galaxy__::control::Str"> <param name="timepoint" type="text" value="" label="timepoint: Str" help="[default: ''] If provided, will cause the results to be stratified by timepoint. Required if using ``subject``."> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> </conditional> </section> </inputs> <outputs> <data name="distribution" format="qza" label="${tool.name} on ${on_string}: distribution.qza" from_work_dir="distribution.qza"/> <data name="stats" format="qza" label="${tool.name} on ${on_string}: stats.qza" from_work_dir="stats.qza"/> <data name="raincloud" format="qzv" label="${tool.name} on ${on_string}: raincloud.qzv" from_work_dir="raincloud.qzv"/> </outputs> <tests> <test> <param name="alpha_diversity" value="alpha_group_significance.test0.alpha-div-faith-pd.qza" ftype="qza"/> <repeat name="metadata"> <conditional name="__q2galaxy__GUI__conditional__metadata__"> <param name="type" value="tsv"/> <param name="source" value="alpha_group_significance.test0.metadata.tsv" ftype="qiime2.tabular"/> </conditional> </repeat> <repeat name="columns"> <param name="element" value="genotype"/> </repeat> <repeat name="columns"> <param name="element" value="donor_status"/> </repeat> <conditional name="__q2galaxy__GUI__conditional__subject__"> <param name="__q2galaxy__GUI__select__" value="__q2galaxy__::control::Str"/> <param name="subject" value="mouse_id"/> </conditional> <conditional name="__q2galaxy__GUI__conditional__timepoint__"> <param name="__q2galaxy__GUI__select__" value="__q2galaxy__::control::Str"/> <param name="timepoint" value="days_post_transplant"/> </conditional> <output name="distribution" ftype="qza"> <assert_contents> <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> <has_line_matching expression="type: Dist1D\[NestedOrdered,\ Matched\]"/> </has_archive_member> </assert_contents> </output> <output name="stats" ftype="qza"/> <output name="raincloud" ftype="qzv"/> <param name="alpha_diversity" value="alpha_group_significance.test0.alpha-div-faith-pd.qza" ftype="qza"/> <repeat name="metadata"> <conditional name="__q2galaxy__GUI__conditional__metadata__"> <param name="type" value="tsv"/> <param name="source" value="alpha_group_significance.test0.metadata.tsv" ftype="qiime2.tabular"/> </conditional> </repeat> <repeat name="columns"> <param name="element" value="genotype"/> </repeat> <repeat name="columns"> <param name="element" value="donor_status"/> </repeat> <output name="distribution" ftype="qza"> <assert_contents> <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> <has_line_matching expression="type: Dist1D\[Multi,\ Independent\]"/> </has_archive_member> </assert_contents> </output> <output name="stats" ftype="qza"/> <output name="raincloud" ftype="qzv"/> </test> </tests> <help> QIIME 2: stats alpha-group-significance ======================================= Alpha group significance test and plot Outputs: -------- :distribution.qza: Dist1D generated by metadata and alpha diversity. :stats.qza: A stats table of the per-group/timepoint results :raincloud.qzv: A visualization of the distribution and statistics | Description: ------------ Will select between Wilcoxon SRT and Mann-Whitney U depending on the presence of repeated measures. Examples: --------- alpha_group_significance_faith_pd ********************************* Using the ``qiime2 stats alpha-group-significance`` tool: #. Set *"alpha_diversity"* to ``#: alpha-div-faith-pd.qza`` #. For *"metadata"*: - Perform the following steps. #. Leave as ``Metadata from TSV`` #. Set *"Metadata Source"* to ``metadata.tsv`` #. For *"columns"*, use the ``+ columns`` button to add the corresponding values: #. Add *"element"* set to ``genotype`` #. Add *"element"* set to ``donor_status`` #. Expand the ``additional options`` section #. Set *"subject"* to ``mouse_id`` #. Set *"timepoint"* to ``days_post_transplant`` #. Press the ``Execute`` button. Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: (Renaming is optional, but it will make any subsequent steps easier to complete.) .. list-table:: :align: left :header-rows: 1 * - History Name - *"Name"* to set (be sure to press ``Save``) * - ``#: qiime2 stats alpha-group-significance [...] : distribution.qza`` - ``dist.qza`` * - ``#: qiime2 stats alpha-group-significance [...] : stats.qza`` - ``stats.qza`` * - ``#: qiime2 stats alpha-group-significance [...] : raincloud.qzv`` - ``raincloud.qzv`` Using the ``qiime2 stats alpha-group-significance`` tool: #. Set *"alpha_diversity"* to ``#: alpha-div-faith-pd.qza`` #. For *"metadata"*: - Perform the following steps. #. Leave as ``Metadata from TSV`` #. Set *"Metadata Source"* to ``metadata.tsv`` #. For *"columns"*, use the ``+ columns`` button to add the corresponding values: #. Add *"element"* set to ``genotype`` #. Add *"element"* set to ``donor_status`` #. Press the ``Execute`` button. Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: (Renaming is optional, but it will make any subsequent steps easier to complete.) .. list-table:: :align: left :header-rows: 1 * - History Name - *"Name"* to set (be sure to press ``Save``) * - ``#: qiime2 stats alpha-group-significance [...] : distribution.qza`` - ``dist2.qza`` * - ``#: qiime2 stats alpha-group-significance [...] : stats.qza`` - ``stats2.qza`` * - ``#: qiime2 stats alpha-group-significance [...] : raincloud.qzv`` - ``raincloud2.qzv`` | </help> <citations> <citation type="doi">10.1038/s41587-019-0209-9</citation> </citations> </tool>