changeset 0:6b6c9ab21674 draft

planemo upload for repository https://gitlab.pasteur.fr/galaxy-team/galaxy-tools/-/tree/master/tools/ccqtl commit 320b3741bd00d1707829822302abe0f0b610981a
author rplanel
date Fri, 30 Jun 2023 14:18:58 +0000
parents
children 2df37db680ad
files ccqtl-permutations.xml macros.xml test-data/ccqtl.loc test-data/ccqtl/indiv_marker/CC_MMnGM_test/cc_crossinfo.csv test-data/ccqtl/indiv_marker/CC_MMnGM_test/cc_geno19.csv test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_foundergeno19.csv test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_gmap19.csv test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_pmap19.csv test-data/format-data/in_covar.csv test-data/format-data/in_phenotypes.csv test-data/format-data/only-female-covar.csv test-data/format-data/only-female-pheno.csv test-data/make-inputs/kloco-female-male.rds test-data/make-inputs/kloco-only-female.rds tool-data/ccqtl.loc.sample tool_data_table_conf.xml.sample tool_data_table_conf.xml.test
diffstat 17 files changed, 14041 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/ccqtl-permutations.xml	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,52 @@
+<tool id="ccqtl_permutations" name="CCQTL: Run permutations" version="@TOOL_VERSION@+@WRAPPER_VERSION@" python_template_version="3.5">
+    <description>to define significance thresholds</description>
+    <macros>
+        <token name="@WRAPPER_VERSION@">galaxy0</token>
+        <import>macros.xml</import>
+    </macros>
+    <edam_topics>
+        <edam_topic>topic_0625</edam_topic>
+        <edam_topic>topic_0160</edam_topic>
+        <edam_topic>topic_3338</edam_topic>
+    </edam_topics>
+    <edam_operations>
+        <edam_operation>operation_3232</edam_operation>
+    </edam_operations>
+    <requirements>
+        <expand macro="requirements"/>
+    </requirements>
+    <command detect_errors="exit_code">
+        <![CDATA[
+        @BASE_CMD@
+            permutations
+            --n_perm ${n_perm}
+            --crossdata ${crossdata}
+            --clean_pr ${clean_pr}
+            --kloco ${kloco}
+            --covar_modmat ${covar_modmat}
+            #if $strat_covar != '':
+                --strat_covar ${strat_covar}
+            #end if
+            ]]>
+    </command>
+    <inputs>
+        <expand macro="crossdata"/>
+        <expand macro="clean_pr"/>
+        <expand macro="kloco"/>
+        <param argument="--n_perm" type="integer" value="2" min="0" max="1000" label="Number of permutations (recommended minimum: 100)"/>
+        <param argument="--covar_modmat" type="data" format="rds" label="covar_modmat object (rds)"/>
+        <param argument="--strat_covar" type="text" value="" label="List of covariates used to stratify permutations on." help="At most three covariates, all must be categorical and previously passed as covariate at the genome_scan step. Typical stratification covariate is sex."/>
+    </inputs>
+    <outputs>
+        <data format="rds" name="operm_lmm" from_work_dir="outfiles/Rdata/operm_lmm.rds" label="${tool.name} on ${on_string}: operm_lmm.rds"/>
+        <data format="rds" name="covar_permstrata" from_work_dir="outfiles/Rdata/covar_permstrata.rds" label="${tool.name} on ${on_string}: covar_permstrata.rds"/>
+        <data format="rds" name="stratcovar_modmat" from_work_dir="outfiles/Rdata/stratcovar_modmat.rds" label="${tool.name} on ${on_string}: stratcovar_modmat.rds"/>
+        <data format="rds" name="nrep_rds" from_work_dir="outfiles/Rdata/nrep.rds" label="${tool.name} on ${on_string}: nrep.rds"/>
+    </outputs>
+    <help>
+        <![CDATA[
+        Compute permutations
+    ]]>
+    </help>
+    <expand macro="citations"/>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,48 @@
+<macros>
+    <token name="@TOOL_VERSION@">0.0.1_beta.2</token>
+    <xml name="requirements">
+        <requirement type="package" version="@TOOL_VERSION@">r-ccqtl</requirement>
+        <yield/>
+    </xml>
+    <token name="@BASE_CMD@">
+        ccqtl --cores \${GALAXY_SLOTS:-1}
+    </token>
+    <xml name="indiv_marker">
+        <param name="indiv_marker" argument="--indiv_geno" type="select" label="Select individual genotypes of each CC mouse at each marker" help="Directory that contains the individual genotypes of each CC mouse at each marker and the funnel codes if applicable.">
+            <options from_data_table="ccqtl">
+                <filter type="static_value" column="1" value="indiv_marker"/>
+                <validator type="no_options" message="No dataset available for the selected input dataset"/>
+            </options>
+        </param>
+    </xml>
+    <xml name="marker_set">
+        <param argument="--marker_set" type="select" label="Select the genetic and physical maps as well as the founder genotypes information" help="Directory that contains the genetic and physical maps as well as the founder genotypes information, for the different combinations of marker set and genome build.">
+            <options from_data_table="ccqtl">
+                <filter type="static_value" column="1" value="marker_set"/>
+                <validator type="no_options" message="No dataset available for the selected input dataset"/>
+            </options>
+        </param>
+    </xml>
+    <xml name="crossdata">
+        <param argument="--crossdata" type="data" format="rds" label="crossdata rds file" help="The cross rds file output from make_inputs"/>
+    </xml>
+    <xml name="kloco">
+        <param argument="--kloco" type="data" format="rds" label="kloco rds file" help="the LOCO kinship rds file output from make_inputs"/>
+    </xml>
+    <xml name="clean_pr">
+        <param argument="--clean_pr" type="data" format="rds" label="clean_pr ds file" help="The cleaned genotype probabilities rds file output from make_inputs"/>
+    </xml>
+    <xml name="citations">
+        <citations>
+            <citation type="bibtex">
+@misc{githubseqtk,
+    author = {Victoire Baillet, Rémi Planel, Vincent Guillemot, Pascal Campagne},
+    year = {2023},
+    title = {ccqtl},
+    publisher = {Gitlab},
+    journal = {Gitlab repository},
+    url = {https://gitlab.pasteur.fr/cc-qtl/ccqtl},
+}</citation>
+        </citations>
+    </xml>
+</macros>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ccqtl.loc	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,10 @@
+# <unique_build_id>	<dbkey>	<display_name>	<build>	<doi>	<description>	<optional_description>	<file_base_path>
+
+#CC_MMnGM	indiv_marker	CC_MMnGM	m38		individual genotypes of each CC mouse at each marker and the funnel codes if applicable. (mega muga and giga muga)		${__HERE__}/ccqtl/indiv_marker/CC_MMnGM_ref
+CC_MMnGM_test	indiv_marker	CC_MMnGM_test	m38		individual genotypes of each CC mouse at each marker and the funnel codes if applicable. (mega muga and giga muga test)		${__HERE__}/ccqtl/indiv_marker/CC_MMnGM_test
+
+#MMnGM_m38	marker_set	MMnGM_m38	m38	https://doi.org/10.6084/m9.figshare.5404762.v2	Marker set for mega muga and giga muga		${__HERE__}/ccqtl/marker_set/MMnGM_m38
+MMnGM_m38_test	marker_set	MMnGM_m38_test	m38	https://doi.org/10.6084/m9.figshare.5404762.v2	Marker set for mega muga and giga muga test		${__HERE__}/ccqtl/marker_set/MMnGM_m38_test
+#MM_m38	marker_set	MM_m38	m38	https://doi.org/10.6084/m9.figshare.5404750.v2	Marker set for mega muga		${__HERE__}/ccqtl/marker_set/MM_m38
+#GM_m38	marker_set	GM_m38	m38	https://doi.org/10.6084/m9.figshare.5404759.v2	Marker set for giga muga$		{__HERE__}/ccqtl/marker_set/GM_m38
+#cc_annotations_m38	cc_annotations	Annotations m38	m38	https://doi.org/10.6084/m9.figshare.5280229.v3,10.6084/m9.figshare.5286019.v8	DNA seq variants segregating among the founders + gene annotation, subsetted to MGI annot		${__HERE__}/ccqtl/annotations/m38
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ccqtl/indiv_marker/CC_MMnGM_test/cc_crossinfo.csv	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,73 @@
+# Cross information for Collaborative Cross (CC) lines inferred from genotypes from Srivastava et al. (2017) doi:10.1534/genetics.116.198838, data at doi:10.5281/zenodo.377036
+# nrow 69
+# ncol 9
+id,A,B,C,D,E,F,G,H
+CC001/Unc,2,5,3,1,4,8,6,7
+CC002/Unc,7,3,4,8,5,1,2,6
+CC003/Unc,5,1,7,2,8,3,6,4
+CC004/TauUnc,3,1,8,7,2,6,5,4
+CC005/TauUnc,4,1,2,3,6,7,8,5
+CC006/TauUnc,7,8,6,1,4,3,2,5
+CC007/Unc,2,1,5,3,8,7,6,4
+CC008/GeniUnc,8,5,2,1,3,7,4,6
+CC009/Unc,5,2,1,7,4,6,8,3
+CC010/GeniUnc,1,5,2,7,6,4,8,3
+CC011/Unc,5,4,2,6,1,7,8,3
+CC012/GeniUnc,2,7,3,6,5,4,8,1
+CC013/GeniUnc,5,4,3,6,8,1,7,2
+CC015/Unc,1,5,6,7,4,2,8,3
+CC016/GeniUnc,8,2,6,5,4,3,7,1
+CC017/Unc,3,7,8,1,6,4,5,2
+CC018/Unc,2,3,6,1,8,7,5,4
+CC019/TauUnc,5,1,2,6,4,8,7,3
+CC020/GeniUnc,7,6,8,4,5,1,3,2
+CC021/Unc,7,3,6,8,4,5,1,2
+CC022/GeniUnc,1,8,4,5,6,2,3,7
+CC023/GeniUnc,4,2,8,5,7,6,3,1
+CC024/GeniUnc,1,2,6,8,5,3,7,4
+CC025/GeniUnc,6,5,3,2,7,8,1,4
+CC026/GeniUnc,6,3,4,7,1,8,2,5
+CC027/GeniUnc,6,4,3,5,1,7,8,2
+CC028/GeniUnc,5,2,4,7,1,3,8,6
+CC029/Unc,8,3,4,1,6,7,2,5
+CC030/GeniUnc,6,5,1,7,2,8,4,3
+CC031/GeniUnc,1,6,5,3,7,2,4,8
+CC032/GeniUnc,1,4,2,3,6,5,8,7
+CC033/GeniUnc,6,5,3,7,1,2,4,8
+CC034/Unc,8,3,7,1,2,5,6,4
+CC035/Unc,6,5,7,8,3,2,4,1
+CC036/Unc,2,6,3,5,8,1,7,4
+CC037/TauUnc,3,4,8,7,6,1,5,2
+CC038/GeniUnc,3,5,4,8,7,1,2,6
+CC039/Unc,7,3,4,2,1,6,5,8
+CC040/TauUnc,4,1,3,5,7,2,6,8
+CC041/TauUnc,1,2,4,7,8,3,6,5
+CC042/GeniUnc,7,3,5,1,6,8,4,2
+CC043/GeniUnc,2,8,1,7,5,3,6,4
+CC044/Unc,3,4,6,2,5,8,1,7
+CC045/GeniUnc,8,2,6,3,4,5,1,7
+CC046/Unc,7,3,4,5,6,8,1,2
+CC047/Unc,1,6,4,7,5,2,8,3
+CC049/TauUnc,2,8,3,1,5,4,7,6
+CC050/Unc,7,8,2,4,3,1,6,5
+CC051/TauUnc,1,2,4,7,8,5,6,3
+CC052/GeniUnc,8,7,6,3,4,2,1,5
+CC053/Unc,6,3,1,5,2,7,8,4
+CC055/TauUnc,7,1,5,8,4,6,2,3
+CC056/GeniUnc,3,8,1,7,5,4,6,2
+CC057/Unc,6,8,4,1,7,3,2,5
+CC058/Unc,3,7,4,8,5,2,1,6
+CC059/TauUnc,1,2,4,6,5,8,7,3
+CC060/Unc,5,1,6,4,7,3,8,2
+CC061/GeniUnc,4,3,5,2,8,1,7,6
+CC062/Unc,6,8,7,2,4,3,1,5
+CC063/Unc,6,8,5,2,3,4,7,1
+CC065/Unc,2,4,8,5,7,3,1,6
+CC068/TauUnc,7,1,3,5,4,6,8,2
+CC070/TauUnc,7,8,6,3,4,2,1,5
+CC071/TauUnc,3,8,4,6,7,1,5,2
+CC072/TauUnc,1,7,2,8,3,4,6,5
+CC073/Unc,2,5,1,3,8,4,6,7
+CC074/Unc,7,8,2,1,6,5,3,4
+CC075/Unc,5,2,6,7,3,8,4,1
+CC076/Unc,3,4,8,5,2,7,6,1
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ccqtl/indiv_marker/CC_MMnGM_test/cc_geno19.csv	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,3061 @@
+# Chromosome 19 genotypes for Collaborative Cross (CC) lines inferred from genotypes from Srivastava et al. (2017) doi:10.1534/genetics.116.198838, data at doi:10.5281/zenodo.377036
+# nrow 3057
+# ncol 70
+marker,CC001/Unc,CC002/Unc,CC003/Unc,CC004/TauUnc,CC005/TauUnc,CC006/TauUnc,CC007/Unc,CC008/GeniUnc,CC009/Unc,CC010/GeniUnc,CC011/Unc,CC012/GeniUnc,CC013/GeniUnc,CC015/Unc,CC016/GeniUnc,CC017/Unc,CC018/Unc,CC019/TauUnc,CC020/GeniUnc,CC021/Unc,CC022/GeniUnc,CC023/GeniUnc,CC024/GeniUnc,CC025/GeniUnc,CC026/GeniUnc,CC027/GeniUnc,CC028/GeniUnc,CC029/Unc,CC030/GeniUnc,CC031/GeniUnc,CC032/GeniUnc,CC033/GeniUnc,CC034/Unc,CC035/Unc,CC036/Unc,CC037/TauUnc,CC038/GeniUnc,CC039/Unc,CC040/TauUnc,CC041/TauUnc,CC042/GeniUnc,CC043/GeniUnc,CC044/Unc,CC045/GeniUnc,CC046/Unc,CC047/Unc,CC049/TauUnc,CC050/Unc,CC051/TauUnc,CC052/GeniUnc,CC053/Unc,CC055/TauUnc,CC056/GeniUnc,CC057/Unc,CC058/Unc,CC059/TauUnc,CC060/Unc,CC061/GeniUnc,CC062/Unc,CC063/Unc,CC065/Unc,CC068/TauUnc,CC070/TauUnc,CC071/TauUnc,CC072/TauUnc,CC073/Unc,CC074/Unc,CC075/Unc,CC076/Unc
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+UNC30604501,B,A,B,A,A,A,A,B,A,B,B,A,A,A,B,A,A,A,B,A,A,A,A,B,B,B,A,A,A,B,B,A,B,A,A,A,A,A,A,A,B,A,A,A,A,B,B,A,B,B,A,B,B,A,A,B,B,A,A,A,A,B,B,A,A,B,B,B,A
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+UNC30604977,A,A,A,A,A,A,A,A,A,A,A,B,A,B,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,B,A,A,B,A,A,B,A,A,A,A,A,A,A,A,A,A,A,A,A,A,A,B,A,A,A,A,A,A,A,A,A,-
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+JAX00090625,B,B,B,A,-,A,A,B,A,B,B,A,A,A,B,A,-,A,B,-,A,A,A,B,B,B,-,A,-,B,B,A,B,-,-,A,A,A,-,A,B,B,A,-,A,B,B,A,B,B,A,B,B,-,A,B,B,A,A,B,A,B,B,B,A,B,B,B,A
+UNC30605071,A,A,A,B,B,B,B,A,B,A,A,B,B,B,A,B,B,B,A,B,B,B,B,A,A,A,B,B,B,A,A,B,A,B,B,B,B,B,B,B,A,A,B,B,B,A,A,B,A,A,B,A,A,B,B,A,A,B,B,A,B,A,A,A,B,A,A,A,B
+UNC30605086,B,B,B,A,A,A,A,B,A,B,B,B,A,B,B,A,A,A,B,A,A,A,A,B,B,B,A,A,A,B,B,A,B,A,A,A,B,A,A,B,B,B,B,A,A,B,B,A,B,B,A,B,B,A,A,B,B,A,B,B,A,B,B,B,A,B,B,B,-
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_foundergeno19.csv	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,3413 @@
+# Founder genotypes (A/B/-) for combined SNPs from MegaMUGA and GigaMUGA
+# nrow 3409
+# ncol 9
+marker,A,B,C,D,E,F,G,H
+UNCHS047038,A,A,A,A,A,B,B,B
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+backupJAX00086312,A,A,A,A,A,B,B,B
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+UNCHS047044,A,B,B,A,B,A,B,A
+UNC190137872,A,B,B,A,B,A,-,A
+B6_rs30503185,B,A,B,B,B,B,B,B
+repB6_rs30503185,B,A,B,B,B,B,B,B
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+++ b/test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_gmap19.csv	Fri Jun 30 14:18:58 2023 +0000
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+# Genetic map for combined SNPs from MegaMUGA and GigaMUGA, chromosome 19
+# nrow 3409
+# ncol 3
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+++ b/test-data/ccqtl/marker_set/MMnGM_m38_test/MMnGM_pmap19.csv	Fri Jun 30 14:18:58 2023 +0000
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/format-data/in_covar.csv	Fri Jun 30 14:18:58 2023 +0000
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/format-data/in_phenotypes.csv	Fri Jun 30 14:18:58 2023 +0000
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/format-data/only-female-covar.csv	Fri Jun 30 14:18:58 2023 +0000
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/format-data/only-female-pheno.csv	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,225 @@
+Individual_ID,Mother_Line,Father_Line,BW_D21,ELISA_Titer
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Binary file test-data/make-inputs/kloco-female-male.rds has changed
Binary file test-data/make-inputs/kloco-only-female.rds has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/ccqtl.loc.sample	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,7 @@
+# <unique_build_id>	<dbkey>	<display_name>	<build>	<doi>	<description>	<optional_description>	<file_base_path>
+
+# CC_MMnGM	indiv_marker	CC_MMnGM	m38		individual genotypes of each CC mouse at each marker and the funnel codes if applicable. (mega muga and giga muga)		tool-data/ccqtl/indiv_marker/CC_MMnGM_ref
+# MMnGM_m38	marker_set	MMnGM_m38	m38	https://figshare.com/articles/dataset/MMnGM_processed_files_zip/5404762/2	Marker set for mega muga and giga muga		tool-data/ccqtl/marker_set/MMnGM_m38
+# MM_m38	marker_set	MM_m38	m38	https://figshare.com/articles/dataset/MM_processed_files_zip/5404750/2	Marker set for mega muga		tool-data/ccqtl/marker_set/MM_m38
+# cc_variants_m38	cc_variants	CC founders variants m38	m38	10.6084/m9.figshare.5280229.v3	DNA seq variants segregating among the founders		tool-data/ccqtl/annotations/m38/cc_variants.sqlite
+# mouse_genes_mgi_m38	genes	Mouse genes mgi m38	m38	10.6084/m9.figshare.5286019.v8	gene annotation, subsetted to MGI annot		tool-data/ccqtl/annotations/m38/mouse_genes_mgi.sqlite
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,9 @@
+<?xml version="1.0"?>
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as
+changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="ccqtl" comment_char="#" allow_duplicate_entries="False">
+        <columns>value, dbkey, name, build, doi, description, optional_description, path</columns>
+        <file path="tool-data/ccqtl.loc" />
+    </table>
+</tables>
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.test	Fri Jun 30 14:18:58 2023 +0000
@@ -0,0 +1,8 @@
+<!-- Use the file tool_data_table_conf.xml.oldlocstyle if you don't want to update your loc files as
+changed in revision 4550:535d276c92bc-->
+<tables>
+    <table name="ccqtl" comment_char="#" allow_duplicate_entries="False">
+        <columns>value, dbkey, name, build, doi, description, optional_description, path</columns>
+        <file path="${__HERE__}/test-data/ccqtl.loc" />
+    </table>
+</tables>
\ No newline at end of file