Mercurial > repos > tduigou > icfree_plates_generator
changeset 3:bd72cb677e4e draft
planemo upload for repository https://github.com/brsynth/icfree-ml commit b655805cb031443000d1da309287481d7a1dd823-dirty
| author | tduigou |
|---|---|
| date | Wed, 24 Jul 2024 08:18:58 +0000 |
| parents | 53a57679cb4a |
| children | 37ba98019165 |
| files | macros.xml plates_generator.xml test-data/converter_proCFPS_parameters.tsv test-data/converter_sampling_concentrations.tsv test-data/converter_sampling_volumes.test-1.tsv test-data/converter_sampling_volumes.test-2.tsv test-data/destination_plate.csv test-data/instructor.access.test-2.csv test-data/instructor.hela.test-2.csv test-data/instructor.test-1.csv test-data/instructor.test-2.csv test-data/instructor_instructions.csv test-data/instructor_volumes_warnings.tsv test-data/plates_designer.destination_plate.test-1.csv test-data/plates_designer.destination_plate.test-2.csv test-data/plates_designer.source_plate.test-1.csv test-data/plates_designer.source_plate.test-2.csv test-data/plates_generator_destination_plate_1.test-1.json test-data/plates_generator_destination_plate_1.test-1.tsv test-data/plates_generator_destination_plate_1.test-2.tsv test-data/plates_generator_source_plate_1.test-1.json test-data/plates_generator_source_plate_1.test-1.tsv test-data/plates_generator_source_plate_1.test-2.tsv test-data/plates_generator_volumes_summary.test-1.tsv test-data/sampling.test-1.csv test-data/source_plate.csv |
| diffstat | 26 files changed, 277 insertions(+), 2455 deletions(-) [+] |
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--- a/macros.xml Wed Feb 14 15:25:58 2024 +0000 +++ b/macros.xml Wed Jul 24 08:18:58 2024 +0000 @@ -1,10 +1,10 @@ <macros> - <token name="@TOOL_VERSION@">2.1.2</token> + <token name="@TOOL_VERSION@">2.4.0</token> <token name="@VERSION_SUFFIX@">0</token> <token name="@LICENSE@">MIT</token> <xml name="requirements"> <requirements> - <requirement type="package" version="@TOOL_VERSION@">icfree</requirement> + <requirement type="package" version="@TOOL_VERSION@">icfree-ml</requirement> <yield/> </requirements> </xml>
--- a/plates_generator.xml Wed Feb 14 15:25:58 2024 +0000 +++ b/plates_generator.xml Wed Jul 24 08:18:58 2024 +0000 @@ -1,152 +1,117 @@ -<tool id="icfree_plates_generator" name="iCFree plates generator" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" license="@LICENCE@"> - <description>Generates a list of Echo-compatible source and destination plates according a set of samples to test</description> +<tool id="icfree_plates_generator" name="iCFree plates designer" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" license="@LICENCE@"> + <description>Prepare source and destination well-plate mappings with advanced options.</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <expand macro="stdio"/> <command detect_errors="exit_code"><![CDATA[ - #set factors = [] + #set well_capacity = [] + #set dead_volumes = [] #for $x in $adv.rep - #silent factors.append(str($x.optimize_well_volumes)) + #silent well_capacity.append(str($x.component_name) + "=" + str($x.well_capacity)) + #silent dead_volumes.append(str($x.component_name) + "=" + str($x.dead_volumes)) #end for - #set sfactors = ' '.join(['"' + $x + '"' for $x in $factors]) - python -m icfree.plates_generator - '$cfps_file' - '$volume_file' - --sample_volume '$adv.sample_volume' - --source_plate_dead_volume '$adv.source_plate_dead_volume' - --dest_plate_dead_volume '$adv.dest_plate_dead_volume' - --dest-starting_well '$adv.dest_starting_well' - --src-starting_well '$adv.src_starting_well' - --nplicate '$adv.nplicate' - --keep-nil-vol '$adv.keep_nil_vol' - --source_plate_well_capacity '$adv.source_plate_well_capacity' - --dest_plate_well_capacity '$adv.dest_plate_well_capacity' - #if len(factors) > 0 - --optimize-well-volumes ${sfactors} + #set swell_capacity = ','.join($well_capacity) + #set sdead_volumes = ','.join($dead_volumes) + + python -m icfree.plate_designer + '$sampling_file' + '$sample_volume' + --start_well_src_plt '$adv.start_well_src_plt' + --start_well_dst_plt '$adv.start_well_dst_plt' + --plate_dims '$adv.plate_dims' + #if len($well_capacity) > 0 + --well_capacity '$swell_capacity' #end if - --plate-dimensions '$adv.plate_dimensions' - --output-folder '.' - --output-format 'tsv' && - mv volumes_summary.tsv '$volumes_summary' - ]]></command> + --default_well_capacity '$adv.default_well_capacity' + #if len($dead_volumes) > 0 + --dead_volumes '$sdead_volumes' + #end if + --default_dead_volume '$adv.default_dead_volume' + --num_replicates '$adv.num_replicates' && + mv source_plate.csv '$output_source_plate' && + mv destination_plate.csv '$output_destination_plate' + ]]></command> <inputs> - <param name="cfps_file" type="data" format="tabular" label="CFPS parameters and features" /> - <param name="volume_file" type="data" format="tabular" label="Volumes" /> - + <param name="sampling_file" type="data" format="csv" label="Sampling file" /> + <param name="sample_volume" type="integer" optional="false" label="Wanted sample volume in the destination plate" /> <section name="adv" title="Advanced Options" expanded="false"> - - <param name="sample_volume" type="integer" value="1000" label="Final sample volume in each well in nL" /> - <param name="source_plate_dead_volume" type="integer" value="15000" label="Dead volume to add in the source plate in nL"/> - <param name="dest_plate_dead_volume" type="integer" value="15000" label="Dead volume to add in the destination plate in nL"/> - - <param name="dest_starting_well" type="text" value="A1" label="Starter well of destination plate to begin filling the 384 well-plate"> + <param name="start_well_src_plt" type="text" value="A1" label="Starting well for the source plate"> <validator type="empty_field" message="Not empty"/> <validator type="regex" message="Some letters followed by a number">^(?:[A-K]{1}([1-9]|1[0-2]))$</validator> </param> - <param name="src_starting_well" type="text" value="A1" label="Starter well of source plate to begin filling the 384 well-plate"> + <param name="start_well_dst_plt" type="text" value="A1" label="Starting well for the destination plate"> <validator type="empty_field" message="Not empty"/> <validator type="regex" message="Some letters followed by a number">^(?:[A-K]{1}([1-9]|1[0-2]))$</validator> </param> - - <param name="nplicate" type="integer" value="3" min="1" max="12" label="Numbers of copies of volume sets"/> - <param name="keep_nil_vol" type="boolean" truevalue="True" falsevalue="False" checked="true" display="checkboxes" label="Keep nil volumes in instructions or not"/> - - <param name="source_plate_well_capacity" type="integer" value="60000" label="Maximum volume capacity of the source plate in nL" /> - <param name="dest_plate_well_capacity" type="integer" value="60000" label="Maximum volume capacity of the dest plate in nL" /> - <param name="plate_dimensions" type="text" value="16x24" label="Dimensions of plate separated by a 'x'"> + <param name="plate_dims" type="text" value="16x24" label="Plate dimensions (Format: NxM)"> <validator type="empty_field" message="Not empty"/> <validator type="regex" message="An 'x' separator is expected">^(?:\d+x\d+)$</validator> </param> - <repeat name="rep" title="Optimize well volumes" min="0"> - <param name="optimize_well_volumes" type="text" value="" label="Save volumes in source plate for all factors"> + <param name="default_well_capacity" type="integer" value="60000" min="1" max="250000" label="Default well capacity in nL for components not specified in well_capacity" /> + <param name="default_dead_volume" type="integer" value="15000" min="1" max="250000" label="Default dead volume in nL for the source plate"/> + <param name="num_replicates" type="integer" value="1" min="1" max="10" label="Number of wanted replicates"/> + <repeat name="rep" title="Specific components"> + <param name="component_name" type="text" value="" label="Component Name"> <validator type="empty_field" message="Not empty"/> </param> + <param name="well_capacity" type="integer" value="60000" min="1" max="250000" label="Well capacity" /> + <param name="dead_volumes" type="integer" value="15000" min="1" max="250000" label="Dead volumes" /> </repeat> - </section> </inputs> <outputs> - <data name="volumes_summary" format="tabular" label="${tool.name} - Volumes" /> - <collection name="output_source_dest" type="list" label="${tool.name} - Source Destination Plate"> - <discover_datasets pattern="(?P<name>source_plate_\d+\.json)$" format="json" /> - <discover_datasets pattern="(?P<name>source_plate_\d+\.tsv)$" format="tabular" /> - <discover_datasets pattern="(?P<name>destination_plate_\d+\.json)$" format="json" /> - <discover_datasets pattern="(?P<name>destination_plate_\d+\.tsv)$" format="tabular" /> - </collection> + <data name="output_source_plate" format="csv" label="${tool.name} - Source" /> + <data name="output_destination_plate" format="csv" label="${tool.name} - Destination" /> </outputs> <tests> - <!-- test 1: check if identical outputs are produced with default parameters --> + <test> + <!-- python -m icfree.plate_designer sampling.test-1.csv 6000 -default_well_capacity 60000 -default_dead_volume 20000 -num_replicates 1 --> + <param name="sampling_file" value="sampling.test-1.csv" /> + <param name="sample_volume" value="6000" /> + <param name="default_well_capacity" value="60000" /> + <param name="default_dead_volume" value="20000" /> + <param name="num_replicates" value="1" /> + <output name="output_source_plate" file="plates_designer.source_plate.test-1.csv" ftype="csv" compare="diff" /> + <output name="output_destination_plate" file="plates_designer.destination_plate.test-1.csv" ftype="csv" compare="diff" /> + </test> <test> - <param name="cfps_file" value="converter_proCFPS_parameters.tsv" /> - <param name="volume_file" value="converter_sampling_concentrations.tsv" /> - <output name="volumes_summary" file="plates_generator_volumes_summary.test-1.tsv" ftype="tabular" compare="diff" /> - <output_collection name="output_source_dest" type="list" count="4"> - <element name="source_plate_1.json" ftype="json"> - <assert_contents> - <has_n_lines n="26"/> - </assert_contents> - </element> - <element name="source_plate_1.tsv" file="plates_generator_source_plate_1.test-1.tsv" ftype="tabular" compare="diff" /> - <element name="destination_plate_1.json" ftype="json"> - <assert_contents> - <has_n_lines n="26"/> - </assert_contents> - </element> - <element name="destination_plate_1.tsv" file="plates_generator_destination_plate_1.test-1.tsv" ftype="tabular" compare="diff" /> - </output_collection> - </test> - <!-- test 2: advance arguments --> - <test> - <param name="cfps_file" value="converter_proCFPS_parameters.tsv" /> - <param name="volume_file" value="converter_sampling_concentrations.tsv" /> - <param name="source_plate_dead_volume" value="1500" /> - <param name="dest_plate_dead_volume" value="1000" /> - <param name="dest_starting_well" value="C3" /> - <param name="src_starting_well" value="A2" /> - <param name="nplicate" value="2" /> - <param name="keep_nil_vol" value="False" /> - <param name="plate_dimensions" value="32x32" /> + <!-- python -m icfree.plate_designer sampling.test-1.csv '5000' -start_well_src_plt 'A1' -start_well_dst_plt 'A1' -plate_dims '16x24' -well_capacity 'Hela lysate=3000,Access prot 50X=4000,Reaction mix=3000,RNA 1ug/uL=2000' -default_well_capacity '60000' -dead_volumes 'Hela lysate=30,Access prot 50X=5,Reaction mix=10,RNA 1ug/uL=20' -default_dead_volume '20000' -num_replicates '2' --> + <param name="sampling_file" value="sampling.test-1.csv" /> + <param name="sample_volume" value="5000" /> + <param name="default_well_capacity" value="60000" /> + <param name="default_dead_volume" value="20000" /> + <param name="num_replicates" value="2" /> <repeat name="rep"> - <param name="optimize_well_volumes" value="RBS" /> + <param name="component_name" value="Hela lysate" /> + <param name="well_capacity" value="3000" /> + <param name="dead_volumes" value="30" /> </repeat> <repeat name="rep"> - <param name="optimize_well_volumes" value="Promoter" /> + <param name="component_name" value="Access prot 50%" /> + <param name="well_capacity" value="4000" /> + <param name="dead_volumes" value="5" /> </repeat> - <output_collection name="output_source_dest" type="list" count="4"> - <element name="destination_plate_1.tsv" file="plates_generator_destination_plate_1.test-2.tsv" ftype="tabular" compare="diff" /> - </output_collection> + <repeat name="rep"> + <param name="component_name" value="Reaction mix" /> + <param name="well_capacity" value="3000" /> + <param name="dead_volumes" value="10" /> + </repeat> + <repeat name="rep"> + <param name="component_name" value="RNA 1ug/uL" /> + <param name="well_capacity" value="2000" /> + <param name="dead_volumes" value="20" /> + </repeat> + <output name="output_source_plate" file="plates_designer.source_plate.test-2.csv" ftype="csv" compare="diff" /> + <output name="output_destination_plate" file="plates_designer.destination_plate.test-2.csv" ftype="csv" compare="diff" /> </test> </tests> <help><![CDATA[ Plates Generator ================ -This module generates a list of source and destination plates according to the set of samples to test. - -Input ------ -* **CFPS Parameters File**: The first column is the parameter (or factor) names. The second column is the maxValue of the parameter that will be used in the sampling. The third column is the concnetration of the stock. The fourth column is the deadVolume of the parameter. This is used to calculate the volume of the parameter that will not be pipetted by the robot (because of viscosity). The fifth column is the specific ratios we want to have for this parameter. If nothing defined, then take ratios given in program options. If one single number is given, then take this number as a const value. -* Volume File**: File containing volumes - -Advanced Options: ------------------ -* **Sample Volume**: Final sample volume in each well in nL (default: 10000) -* **Source Plate Dead Volume**: dead volume to add in the source plate in nL (default: 15000) -* **Destination Plate Dead Volume**: dead volume to add in the dest plate in nL (default: 15000) -* **Destination Starting Well**: starter well of destination plate to begin filling the 384 well-plate. (default: A1) -* **Source Starting Well**: starter well of source plate to begin filling the 384 well-plate. (default: A1) -* **Number of replicate**: Numbers of copies of volume sets (default: 3) -* **Keep nil volumes**: Keep nil volumes in instructions or not (default: yes) -* **Source Plate Well Capacity**: Maximum volume capacity of the source plate in nL (default: 60000) -* **Destiantion Plate Well Capacity**: Maximum volume capacity of the dest plate in nL (default: 60000) -* **Optimize Well Volumes**: Save volumes in source plate for all factors. It may trigger more volume pipetting warnings. If list of factors is given (separated by blanks), save: only for these ones (default: []). -* **Plate Dimensions**: Dimensions of plate separated by a 'x', e.g. nb_rows x nb_cols (default: 16x24). - -Output ------- -* **Collection**: Collection of files describing source/destination plates/wells +Prepare source and destination well-plate mappings with advanced options. ]]></help> <expand macro="creator"/> <expand macro="citation"/>
--- a/test-data/converter_proCFPS_parameters.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,17 +0,0 @@ -Parameter maxValue stockConcentration deadVolume Ratios -Mg-glutamate 4 168 0 0.0 0.1 0.3 0.5 1.0 -k-glutamate 80 3360 0 -CoA 0.26 210 0 -3-PGA 30 1400 0 -NTP 1.5 630 0 -NAD 0.33 138.6 0 -Folinic acid 0.068 28.56 0 -Spermidine 1 420 0 -tRNA 0.2 84 0 -Amino acids 1.5 6 0 -CAMP 0.75 200 0 -Extract 30 300 2000 1 -HEPES 50 2100 0 1 -PEG 2 200 4000 1 -Promoter 10 300 0 1 -RBS 10 200 0 1 \ No newline at end of file
--- a/test-data/converter_sampling_concentrations.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ -Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS -0.4 44.8 0.0 21.6 0.6 0.0 0.049 0.2 0.112 1.2 0.15 30.0 50.0 2.0 10.0 10.0 -4.0 32.0 0.104 6.0 0.3 0.264 0.014 0.56 0.08 0.3 0.6 30.0 50.0 2.0 10.0 10.0 -4.0 0.0 0.146 0.0 0.0 0.132 0.027 0.8 0.04 0.6 0.75 30.0 50.0 2.0 10.0 10.0 -0.0 32.0 0.052 6.0 1.08 0.132 0.054 0.0 0.144 1.5 0.6 30.0 50.0 2.0 10.0 10.0 -2.0 64.0 0.0 12.0 1.5 0.066 0.044 0.64 0.16 0.3 0.3 30.0 50.0 2.0 10.0 10.0 -0.4 80.0 0.0 30.0 0.84 0.066 0.038 0.4 0.0 1.2 0.48 30.0 50.0 2.0 10.0 10.0 -2.0 51.2 0.208 12.0 0.3 0.066 0.038 0.56 0.08 1.2 0.3 30.0 50.0 2.0 10.0 10.0 -2.0 32.0 0.104 24.0 1.5 0.33 0.014 0.8 0.08 0.3 0.15 30.0 50.0 2.0 10.0 10.0 -0.0 44.8 0.208 21.6 1.2 0.238 0.038 0.2 0.0 0.96 0.3 30.0 50.0 2.0 10.0 10.0 -2.0 16.0 0.208 24.0 0.84 0.264 0.014 1.0 0.04 0.84 0.75 30.0 50.0 2.0 10.0 10.0 -1.2 57.6 0.0 24.0 0.96 0.132 0.027 0.2 0.112 0.3 0.3 30.0 50.0 2.0 10.0 10.0 -1.2 16.0 0.166 21.6 0.3 0.066 0.068 0.4 0.144 0.6 0.3 30.0 50.0 2.0 10.0 10.0 -1.2 16.0 0.26 19.2 0.96 0.066 0.044 0.4 0.08 1.2 0.15 30.0 50.0 2.0 10.0 10.0 -0.4 80.0 0.104 30.0 0.84 0.33 0.068 0.8 0.112 1.08 0.0 30.0 50.0 2.0 10.0 10.0 -4.0 32.0 0.104 24.0 1.2 0.0 0.038 0.2 0.112 0.3 0.54 30.0 50.0 2.0 10.0 10.0 -4.0 64.0 0.104 24.0 1.08 0.185 0.049 0.72 0.08 0.3 0.42 30.0 50.0 2.0 10.0 10.0 -1.2 0.0 0.208 0.0 1.08 0.238 0.049 0.56 0.2 0.96 0.75 30.0 50.0 2.0 10.0 10.0 -2.0 16.0 0.208 6.0 0.84 0.211 0.038 0.2 0.128 1.2 0.6 30.0 50.0 2.0 10.0 10.0 -4.0 64.0 0.146 16.8 0.96 0.132 0.049 0.4 0.0 0.84 0.42 30.0 50.0 2.0 10.0 10.0 -1.2 44.8 0.208 12.0 1.08 0.264 0.068 0.0 0.16 0.6 0.6 30.0 50.0 2.0 10.0 10.0 -2.0 44.8 0.26 16.8 1.2 0.185 0.044 0.72 0.0 0.6 0.3 30.0 50.0 2.0 10.0 10.0 -4.0 0.0 0.052 12.0 0.6 0.132 0.027 0.4 0.04 0.6 0.0 30.0 50.0 2.0 10.0 10.0 -4.0 32.0 0.052 16.8 1.2 0.264 0.014 0.2 0.08 0.3 0.3 30.0 50.0 2.0 10.0 10.0 -2.0 44.8 0.052 16.8 0.3 0.066 0.049 0.8 0.04 1.2 0.48 30.0 50.0 2.0 10.0 10.0 -4.0 80.0 0.0 16.8 0.3 0.264 0.054 0.56 0.16 0.6 0.15 30.0 50.0 2.0 10.0 10.0 -1.2 44.8 0.0 6.0 0.6 0.211 0.054 0.56 0.144 0.0 0.15 30.0 50.0 2.0 10.0 10.0 -2.0 0.0 0.146 21.6 1.2 0.0 0.027 0.0 0.04 0.84 0.6 30.0 50.0 2.0 10.0 10.0 -0.4 16.0 0.166 6.0 0.3 0.33 0.068 1.0 0.16 0.6 0.54 30.0 50.0 2.0 10.0 10.0 -1.2 57.6 0.208 24.0 0.3 0.211 0.038 0.4 0.2 1.08 0.75 30.0 50.0 2.0 10.0 10.0 -0.4 80.0 0.052 30.0 0.3 0.264 0.0 0.72 0.128 1.08 0.0 30.0 50.0 2.0 10.0 10.0 -2.0 32.0 0.146 12.0 1.2 0.211 0.054 0.64 0.08 0.96 0.6 30.0 50.0 2.0 10.0 10.0 -1.2 51.2 0.146 12.0 0.6 0.132 0.014 0.2 0.0 1.08 0.54 30.0 50.0 2.0 10.0 10.0 -2.0 64.0 0.052 30.0 1.2 0.066 0.068 0.8 0.128 0.3 0.15 30.0 50.0 2.0 10.0 10.0 -4.0 0.0 0.208 0.0 0.3 0.0 0.044 0.0 0.16 0.6 0.15 30.0 50.0 2.0 10.0 10.0 -1.2 64.0 0.26 0.0 0.0 0.264 0.0 0.56 0.04 1.2 0.54 30.0 50.0 2.0 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--- a/test-data/converter_sampling_volumes.test-1.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ -Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS -2.5 12.5 2.5 15.0 0.0 0.0 2.5 0.0 2.5 200.0 0.0 100.0 25.0 10.0 32.5 50.0 -25.0 10.0 2.5 5.0 0.0 2.5 0.0 2.5 0.0 50.0 2.5 100.0 25.0 10.0 32.5 50.0 -25.0 0.0 2.5 0.0 0.0 0.0 0.0 2.5 0.0 100.0 5.0 100.0 25.0 10.0 32.5 50.0 -0.0 10.0 2.5 5.0 2.5 0.0 2.5 0.0 2.5 250.0 2.5 100.0 25.0 10.0 32.5 50.0 -12.5 20.0 2.5 7.5 2.5 0.0 2.5 2.5 2.5 50.0 2.5 100.0 25.0 10.0 32.5 50.0 -2.5 25.0 2.5 22.5 2.5 0.0 2.5 0.0 0.0 200.0 2.5 100.0 25.0 10.0 32.5 50.0 -12.5 15.0 2.5 7.5 0.0 0.0 2.5 2.5 0.0 200.0 2.5 100.0 25.0 10.0 32.5 50.0 -12.5 10.0 2.5 17.5 2.5 2.5 0.0 2.5 0.0 50.0 0.0 100.0 25.0 10.0 32.5 50.0 -0.0 12.5 2.5 15.0 2.5 2.5 2.5 0.0 0.0 160.0 2.5 100.0 25.0 10.0 32.5 50.0 -12.5 5.0 2.5 17.5 2.5 2.5 0.0 2.5 0.0 140.0 5.0 100.0 25.0 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--- a/test-data/converter_sampling_volumes.test-2.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ -Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS -0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 20.0 2.5 10.0 2.5 2.5 2.5 5.0 -2.5 0.0 2.5 0.0 2.5 2.5 2.5 2.5 2.5 5.0 2.5 10.0 2.5 2.5 2.5 5.0 -2.5 0.0 2.5 0.0 2.5 2.5 2.5 2.5 2.5 10.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 0.0 2.5 0.0 2.5 2.5 2.5 2.5 2.5 25.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 2.5 2.5 0.0 2.5 2.5 2.5 2.5 2.5 5.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 20.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 2.5 2.5 0.0 2.5 2.5 2.5 2.5 2.5 20.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 5.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 15.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 15.0 2.5 10.0 2.5 2.5 2.5 5.0 -0.0 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 5.0 2.5 10.0 2.5 2.5 2.5 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/destination_plate.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,4 @@ +Well,Hela lysate,Access prot 50%,Reaction mix,RNA 1ug/uL,Water +A1,492.5,25.0,310.0,152.5,5020.0 +B1,970.0,165.0,257.5,112.5,4495.0 +C1,537.5,325.0,192.5,185.0,4760.0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/instructor.access.test-2.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,7 @@ +Source Plate Name,Source Plate Type,Source Well,Destination Plate Name,Destination Well,Transfer Volume,Sample ID +Source[1],384PP_AQ_GP3,C1,Destination[1],A1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],B1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],C1,325.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],D1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],E1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],F1,325.0,Access prot 50%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/instructor.hela.test-2.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,11 @@ +Source Plate Name,Source Plate Type,Source Well,Destination Plate Name,Destination Well,Transfer Volume,Sample ID +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],A1,492.5,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],B1,500.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],B1,470.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],C1,500.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],C1,37.5,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],D1,492.5,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],E1,500.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],E1,470.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],F1,500.0,Hela lysate +Source[1],384PP_AQ_CP,{A1;B1},Destination[1],F1,37.5,Hela lysate
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/instructor.test-1.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,77 @@ +Source Plate Name,Source Plate Type,Source Well,Destination Plate Name,Destination Well,Transfer Volume,Sample ID +Source[1],384PP_AQ_GP3,C1,Destination[1],A1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],B1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],C1,325.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],D1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],E1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],F1,325.0,Access prot 50% +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],A1,492.5,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],B1,500.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],B1,470.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],C1,500.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],C1,37.5,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],D1,492.5,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],E1,500.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],E1,470.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],F1,500.0,Hela lysate +Source[1],384PP_AQ_GP3,{A1;B1},Destination[1],F1,37.5,Hela lysate +Source[1],384PP_AQ_GP3,E1,Destination[1],A1,152.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],B1,112.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],C1,185.0,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],D1,152.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],E1,112.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],F1,185.0,RNA 1ug/uL +Source[1],384PP_AQ_GP3,D1,Destination[1],A1,310.0,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],B1,257.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],C1,192.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],D1,310.0,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],E1,257.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],F1,192.5,Reaction mix +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,20.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,495.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,260.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,20.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,495.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,260.0,Water
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/instructor.test-2.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,67 @@ +Source Plate Name,Source Plate Type,Source Well,Destination Plate Name,Destination Well,Transfer Volume,Sample ID +Source[1],384PP_AQ_GP3,C1,Destination[1],A1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],B1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],C1,325.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],D1,25.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],E1,165.0,Access prot 50% +Source[1],384PP_AQ_GP3,C1,Destination[1],F1,325.0,Access prot 50% +Source[1],384PP_AQ_GP3,E1,Destination[1],A1,152.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],B1,112.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],C1,185.0,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],D1,152.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],E1,112.5,RNA 1ug/uL +Source[1],384PP_AQ_GP3,E1,Destination[1],F1,185.0,RNA 1ug/uL +Source[1],384PP_AQ_GP3,D1,Destination[1],A1,310.0,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],B1,257.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],C1,192.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],D1,310.0,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],E1,257.5,Reaction mix +Source[1],384PP_AQ_GP3,D1,Destination[1],F1,192.5,Reaction mix +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],A1,20.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],B1,495.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],C1,260.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],D1,20.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],E1,495.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,500.0,Water +Source[1],384PP_AQ_GP3,F1,Destination[1],F1,260.0,Water
--- a/test-data/instructor_instructions.csv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1701 +0,0 @@ -Source Plate Name,Source Plate Type,Source Well,Destination Plate Name,Destination Well,Transfer Volume,Sample ID -Source[1],384PP_AQ_GP3,A1,Destination[1],A1,0.4,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],B1,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],C1,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],D1,0.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],E1,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],F1,0.4,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],G1,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],H1,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],I1,0.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],J1,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],K1,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],L1,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],M1,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],N1,0.4,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],O1,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],P1,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],A2,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],B2,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],C2,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],D2,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],E2,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],F2,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],G2,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],H2,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],I2,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],J2,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],K2,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],L2,0.4,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],M2,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],N2,0.4,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],O2,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],P2,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],A3,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],B3,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],C3,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],D3,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],E3,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],F3,1.2,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],G3,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],H3,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],I3,2.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],J3,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],K3,4.0,Mg-glutamate -Source[1],384PP_AQ_GP3,A1,Destination[1],L3,0.4,Mg-glutamate 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-Source[1],384PP_AQ_GP3,J1,Destination[1],I3,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],J3,0.96,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],K3,1.08,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],L3,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],M3,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],N3,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],O3,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],P3,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],A4,1.08,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],B4,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],C4,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],D4,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],E4,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],F4,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],G4,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],H4,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],I4,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],J4,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],K4,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],L4,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],M4,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],N4,0.96,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],O4,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],P4,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],A5,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],B5,1.08,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],C5,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],D5,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],E5,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],F5,1.08,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],G5,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],H5,0.96,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],I5,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],J5,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],K5,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],L5,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],M5,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],N5,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],O5,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],P5,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],A6,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],B6,0.96,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],C6,0.96,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],D6,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],E6,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],F6,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],G6,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],H6,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],I6,1.2,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],J6,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],K6,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],L6,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],M6,1.5,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],N6,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],O6,0.6,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],P6,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],A7,0.84,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],B7,0.3,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],C7,0.0,Amino acids -Source[1],384PP_AQ_GP3,J1,Destination[1],D7,0.0,Amino acids -Source[1],384PP_AQ_GP3,K1,Destination[1],A1,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B1,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C1,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D1,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E1,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F1,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G1,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H1,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I1,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J1,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K1,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L1,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M1,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N1,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O1,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P1,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A2,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B2,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C2,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D2,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E2,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F2,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G2,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H2,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I2,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J2,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K2,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L2,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M2,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N2,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O2,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P2,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A3,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B3,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C3,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D3,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E3,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F3,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G3,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H3,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I3,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J3,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K3,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L3,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M3,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N3,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O3,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P3,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A4,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B4,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C4,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D4,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E4,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F4,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G4,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H4,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I4,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J4,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K4,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L4,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M4,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N4,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O4,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P4,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A5,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B5,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C5,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D5,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E5,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F5,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G5,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H5,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I5,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J5,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K5,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L5,0.54,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M5,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N5,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O5,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P5,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A6,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B6,0.75,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C6,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D6,0.48,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],E6,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],F6,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],G6,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],H6,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],I6,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],J6,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],K6,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],L6,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],M6,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],N6,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],O6,0.0,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],P6,0.3,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],A7,0.6,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],B7,0.15,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],C7,0.42,CAMP -Source[1],384PP_AQ_GP3,K1,Destination[1],D7,0.42,CAMP -Source[1],384PP_AQ_GP3,L1,Destination[1],A1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P1,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P2,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P3,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P4,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P5,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],E6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],F6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],G6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],H6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],I6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],J6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],K6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],L6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],M6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],N6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],O6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],P6,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],A7,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],B7,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],C7,30.0,Extract -Source[1],384PP_AQ_GP3,L1,Destination[1],D7,30.0,Extract -Source[1],384PP_AQ_GP3,M1,Destination[1],A1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],B1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],C1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],D1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],E1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],F1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],G1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],H1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],I1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],J1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],K1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],L1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],M1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],N1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],O1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],P1,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],A2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],B2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],C2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],D2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],E2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],F2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],G2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],H2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],I2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],J2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],K2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],L2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],M2,50.0,HEPES -Source[1],384PP_AQ_GP3,M1,Destination[1],N2,50.0,HEPES 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-Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],J1,852.044,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],K1,813.169,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],L1,857.156,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],M1,858.44,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],N1,784.2660000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],O1,835.506,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],P1,803.062,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],A2,892.755,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],B2,870.575,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],C2,810.2529999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],D2,837.02,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],E2,831.091,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],F2,880.149,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],G2,842.79,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],H2,831.413,Water 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-Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],G3,851.477,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],H3,848.1179999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],I3,823.451,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],J3,852.947,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],K3,884.426,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],L3,832.8620000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],M3,788.242,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],N3,853.1610000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],O3,829.3879999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],P3,847.24,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],A4,798.9939999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],B4,848.975,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],C4,847.152,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],D4,861.222,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],E4,823.245,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],F4,822.374,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],G4,867.89,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],H4,851.58,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],I4,834.097,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],J4,812.83,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],K4,861.36,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],L4,790.202,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],M4,818.37,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],N4,811.5450000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],O4,875.917,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],P4,811.105,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],A5,845.587,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],B5,871.74,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],C5,824.565,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],D5,793.534,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],E5,835.396,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],F5,858.627,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],G5,851.634,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],H5,882.293,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],I5,826.076,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],J5,854.65,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],K5,841.062,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],L5,862.0609999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],M5,834.2,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],N5,782.6220000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],O5,877.639,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],P5,848.375,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],A6,855.529,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],B6,817.104,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],C6,831.7760000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],D6,792.995,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],E6,894.233,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],F6,813.8009999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],G6,832.139,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],H6,832.266,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],I6,817.2339999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],J6,886.3679999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],K6,826.279,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],L6,844.238,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],M6,854.306,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],N6,830.6220000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],O6,856.086,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],P6,829.8879999999999,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],A7,853.505,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],B7,847.1320000000001,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],C7,858.113,Water -Source[1],384PP_AQ_GP3,{A2;B2},Destination[1],D7,834.598,Water
--- a/test-data/instructor_volumes_warnings.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,12 +0,0 @@ -Parameter Min Max Plate -Mg-glutamate 0.4 plates_generator_destination_plate_1.json -CoA 0.052 plates_generator_destination_plate_1.json -3-PGA 6.0 plates_generator_destination_plate_1.json -NTP 0.3 plates_generator_destination_plate_1.json -NAD 0.066 plates_generator_destination_plate_1.json -Folinic acid 0.014 plates_generator_destination_plate_1.json -Spermidine 0.2 plates_generator_destination_plate_1.json -tRNA 0.04 plates_generator_destination_plate_1.json -Amino acids 0.3 plates_generator_destination_plate_1.json -CAMP 0.15 plates_generator_destination_plate_1.json -PEG 2.0 plates_generator_destination_plate_1.json
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/plates_designer.destination_plate.test-1.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,4 @@ +Well,Hela lysate,Access prot 50%,Reaction mix,RNA 1ug/uL,Water +A1,492.5,25.0,310.0,152.5,5020.0 +B1,970.0,165.0,257.5,112.5,4495.0 +C1,537.5,325.0,192.5,185.0,4760.0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/plates_designer.destination_plate.test-2.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,7 @@ +Well,Hela lysate,Access prot 50%,Reaction mix,RNA 1ug/uL,Water +A1,492.5,25.0,310.0,152.5,4020.0 +B1,970.0,165.0,257.5,112.5,3495.0 +C1,537.5,325.0,192.5,185.0,3760.0 +D1,492.5,25.0,310.0,152.5,4020.0 +E1,970.0,165.0,257.5,112.5,3495.0 +F1,537.5,325.0,192.5,185.0,3760.0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/plates_designer.source_plate.test-1.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,6 @@ +Well,Access prot 50%,Hela lysate,RNA 1ug/uL,Reaction mix,Water +A1,0.0,22000.0,0.0,0.0,0.0 +B1,20515.0,0.0,0.0,0.0,0.0 +C1,0.0,0.0,0.0,20760.0,0.0 +D1,0.0,0.0,20450.0,0.0,0.0 +E1,0.0,0.0,0.0,0.0,34275.0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/plates_designer.source_plate.test-2.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,7 @@ +Well,Access prot 50%,Hela lysate,RNA 1ug/uL,Reaction mix,Water +A1,0.0,3000.0,0.0,0.0,0.0 +B1,0.0,1060.0,0.0,0.0,0.0 +C1,21030.0,0.0,0.0,0.0,0.0 +D1,0.0,0.0,0.0,1530.0,0.0 +E1,0.0,0.0,920.0,0.0,0.0 +F1,0.0,0.0,0.0,0.0,42550.0
--- a/test-data/plates_generator_destination_plate_1.test-1.json Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,26 +0,0 @@ -{ - "Dimensions": "16x24", - "deadVolume": 0, - "Well capacity": 60000, - "Current well": "E7", - "Parameters": [ - "Spermidine", - "CAMP", - "Extract", - "CoA", - "HEPES", - "Folinic acid", - "k-glutamate", - "PEG", - "Mg-glutamate", - "Promoter", - "RBS", - "Amino acids", - "NTP", - "Water", - "NAD", - "3-PGA", - "tRNA" - ], - "Wells": "plates_generator_destination_plate_1.tsv" -}
--- a/test-data/plates_generator_destination_plate_1.test-1.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ -Well Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS Water -A1 0.4 44.8 0.0 21.6 0.6 0.0 0.049 0.2 0.112 1.2 0.15 30.0 50.0 2.0 10.0 10.0 828.889 -B1 4.0 32.0 0.104 6.0 0.3 0.264 0.014 0.56 0.08 0.3 0.6 30.0 50.0 2.0 10.0 10.0 853.778 -C1 4.0 0.0 0.146 0.0 0.0 0.132 0.027 0.8 0.04 0.6 0.75 30.0 50.0 2.0 10.0 10.0 891.505 -D1 0.0 32.0 0.052 6.0 1.08 0.132 0.054 0.0 0.144 1.5 0.6 30.0 50.0 2.0 10.0 10.0 856.438 -E1 2.0 64.0 0.0 12.0 1.5 0.066 0.044 0.64 0.16 0.3 0.3 30.0 50.0 2.0 10.0 10.0 816.99 -F1 0.4 80.0 0.0 30.0 0.84 0.066 0.038 0.4 0.0 1.2 0.48 30.0 50.0 2.0 10.0 10.0 784.576 -G1 2.0 51.2 0.208 12.0 0.3 0.066 0.038 0.56 0.08 1.2 0.3 30.0 50.0 2.0 10.0 10.0 830.048 -H1 2.0 32.0 0.104 24.0 1.5 0.33 0.014 0.8 0.08 0.3 0.15 30.0 50.0 2.0 10.0 10.0 836.722 -I1 0.0 44.8 0.208 21.6 1.2 0.238 0.038 0.2 0.0 0.96 0.3 30.0 50.0 2.0 10.0 10.0 828.456 -J1 2.0 16.0 0.208 24.0 0.84 0.264 0.014 1.0 0.04 0.84 0.75 30.0 50.0 2.0 10.0 10.0 852.044 -K1 1.2 57.6 0.0 24.0 0.96 0.132 0.027 0.2 0.112 0.3 0.3 30.0 50.0 2.0 10.0 10.0 813.169 -L1 1.2 16.0 0.166 21.6 0.3 0.066 0.068 0.4 0.144 0.6 0.3 30.0 50.0 2.0 10.0 10.0 857.156 -M1 1.2 16.0 0.26 19.2 0.96 0.066 0.044 0.4 0.08 1.2 0.15 30.0 50.0 2.0 10.0 10.0 858.44 -N1 0.4 80.0 0.104 30.0 0.84 0.33 0.068 0.8 0.112 1.08 0.0 30.0 50.0 2.0 10.0 10.0 784.2660000000001 -O1 4.0 32.0 0.104 24.0 1.2 0.0 0.038 0.2 0.112 0.3 0.54 30.0 50.0 2.0 10.0 10.0 835.506 -P1 4.0 64.0 0.104 24.0 1.08 0.185 0.049 0.72 0.08 0.3 0.42 30.0 50.0 2.0 10.0 10.0 803.062 -A2 1.2 0.0 0.208 0.0 1.08 0.238 0.049 0.56 0.2 0.96 0.75 30.0 50.0 2.0 10.0 10.0 892.755 -B2 2.0 16.0 0.208 6.0 0.84 0.211 0.038 0.2 0.128 1.2 0.6 30.0 50.0 2.0 10.0 10.0 870.575 -C2 4.0 64.0 0.146 16.8 0.96 0.132 0.049 0.4 0.0 0.84 0.42 30.0 50.0 2.0 10.0 10.0 810.2529999999999 -D2 1.2 44.8 0.208 12.0 1.08 0.264 0.068 0.0 0.16 0.6 0.6 30.0 50.0 2.0 10.0 10.0 837.02 -E2 2.0 44.8 0.26 16.8 1.2 0.185 0.044 0.72 0.0 0.6 0.3 30.0 50.0 2.0 10.0 10.0 831.091 -F2 4.0 0.0 0.052 12.0 0.6 0.132 0.027 0.4 0.04 0.6 0.0 30.0 50.0 2.0 10.0 10.0 880.149 -G2 4.0 32.0 0.052 16.8 1.2 0.264 0.014 0.2 0.08 0.3 0.3 30.0 50.0 2.0 10.0 10.0 842.79 -H2 2.0 44.8 0.052 16.8 0.3 0.066 0.049 0.8 0.04 1.2 0.48 30.0 50.0 2.0 10.0 10.0 831.413 -I2 4.0 80.0 0.0 16.8 0.3 0.264 0.054 0.56 0.16 0.6 0.15 30.0 50.0 2.0 10.0 10.0 795.1120000000001 -J2 1.2 44.8 0.0 6.0 0.6 0.211 0.054 0.56 0.144 0.0 0.15 30.0 50.0 2.0 10.0 10.0 844.281 -K2 2.0 0.0 0.146 21.6 1.2 0.0 0.027 0.0 0.04 0.84 0.6 30.0 50.0 2.0 10.0 10.0 871.547 -L2 0.4 16.0 0.166 6.0 0.3 0.33 0.068 1.0 0.16 0.6 0.54 30.0 50.0 2.0 10.0 10.0 872.436 -M2 1.2 57.6 0.208 24.0 0.3 0.211 0.038 0.4 0.2 1.08 0.75 30.0 50.0 2.0 10.0 10.0 812.0129999999999 -N2 0.4 80.0 0.052 30.0 0.3 0.264 0.0 0.72 0.128 1.08 0.0 30.0 50.0 2.0 10.0 10.0 785.056 -O2 2.0 32.0 0.146 12.0 1.2 0.211 0.054 0.64 0.08 0.96 0.6 30.0 50.0 2.0 10.0 10.0 848.1089999999999 -P2 1.2 51.2 0.146 12.0 0.6 0.132 0.014 0.2 0.0 1.08 0.54 30.0 50.0 2.0 10.0 10.0 830.8879999999999 -A3 2.0 64.0 0.052 30.0 1.2 0.066 0.068 0.8 0.128 0.3 0.15 30.0 50.0 2.0 10.0 10.0 799.236 -B3 4.0 0.0 0.208 0.0 0.3 0.0 0.044 0.0 0.16 0.6 0.15 30.0 50.0 2.0 10.0 10.0 892.538 -C3 1.2 64.0 0.26 0.0 0.0 0.264 0.0 0.56 0.04 1.2 0.54 30.0 50.0 2.0 10.0 10.0 829.9359999999999 -D3 2.0 64.0 0.052 6.0 0.96 0.185 0.038 0.0 0.112 0.84 0.6 30.0 50.0 2.0 10.0 10.0 823.213 -E3 4.0 64.0 0.187 6.0 0.3 0.185 0.027 0.4 0.112 0.3 0.15 30.0 50.0 2.0 10.0 10.0 822.3389999999999 -F3 1.2 51.2 0.052 24.0 0.6 0.185 0.027 0.4 0.08 0.6 0.3 30.0 50.0 2.0 10.0 10.0 819.356 -G3 4.0 16.0 0.104 24.0 0.0 0.185 0.054 0.4 0.04 1.2 0.54 30.0 50.0 2.0 10.0 10.0 851.477 -H3 2.0 32.0 0.26 12.0 1.08 0.238 0.044 0.2 0.08 1.5 0.48 30.0 50.0 2.0 10.0 10.0 848.1179999999999 -I3 2.0 64.0 0.187 6.0 0.6 0.33 0.038 0.8 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16.8 1.2 0.066 0.068 0.4 0.16 0.3 0.48 30.0 50.0 2.0 10.0 10.0 861.222 -E4 1.2 51.2 0.187 19.2 1.5 0.264 0.014 0.0 0.2 0.84 0.15 30.0 50.0 2.0 10.0 10.0 823.245 -F4 2.0 64.0 0.146 6.0 1.5 0.066 0.014 0.56 0.2 0.84 0.3 30.0 50.0 2.0 10.0 10.0 822.374 -G4 0.4 16.0 0.104 12.0 0.3 0.132 0.054 0.0 0.04 0.6 0.48 30.0 50.0 2.0 10.0 10.0 867.89 -H4 4.0 16.0 0.146 24.0 0.6 0.0 0.014 0.8 0.08 0.3 0.48 30.0 50.0 2.0 10.0 10.0 851.58 -I4 4.0 44.8 0.052 12.0 0.96 0.211 0.0 0.0 0.08 1.5 0.3 30.0 50.0 2.0 10.0 10.0 834.097 -J4 4.0 57.6 0.166 19.2 0.96 0.066 0.0 0.8 0.128 1.5 0.75 30.0 50.0 2.0 10.0 10.0 812.83 -K4 0.4 16.0 0.166 16.8 0.3 0.0 0.014 1.0 0.16 1.5 0.3 30.0 50.0 2.0 10.0 10.0 861.36 -L4 2.0 80.0 0.0 24.0 0.0 0.264 0.014 0.8 0.0 0.3 0.42 30.0 50.0 2.0 10.0 10.0 790.202 -M4 2.0 57.6 0.052 16.8 0.6 0.066 0.014 0.72 0.128 1.5 0.15 30.0 50.0 2.0 10.0 10.0 818.37 -N4 2.0 57.6 0.104 24.0 0.3 0.132 0.027 0.8 0.112 0.96 0.42 30.0 50.0 2.0 10.0 10.0 811.5450000000001 -O4 0.0 0.0 0.187 19.2 0.84 0.066 0.0 0.2 0.0 0.84 0.75 30.0 50.0 2.0 10.0 10.0 875.917 -P4 2.0 64.0 0.26 16.8 0.96 0.264 0.027 0.64 0.144 1.2 0.6 30.0 50.0 2.0 10.0 10.0 811.105 -A5 1.2 32.0 0.104 16.8 0.6 0.132 0.027 0.72 0.08 0.6 0.15 30.0 50.0 2.0 10.0 10.0 845.587 -B5 1.2 16.0 0.052 6.0 0.84 0.33 0.014 0.0 0.144 1.08 0.6 30.0 50.0 2.0 10.0 10.0 871.74 -C5 1.2 64.0 0.208 6.0 0.6 0.238 0.049 0.72 0.0 0.0 0.42 30.0 50.0 2.0 10.0 10.0 824.565 -D5 2.0 80.0 0.052 19.2 1.5 0.185 0.027 0.4 0.112 0.84 0.15 30.0 50.0 2.0 10.0 10.0 793.534 -E5 2.0 57.6 0.052 0.0 1.08 0.238 0.014 0.64 0.08 0.6 0.3 30.0 50.0 2.0 10.0 10.0 835.396 -F5 2.0 16.0 0.166 19.2 0.0 0.185 0.054 0.56 0.128 1.08 0.0 30.0 50.0 2.0 10.0 10.0 858.627 -G5 0.0 32.0 0.104 12.0 0.84 0.0 0.038 0.64 0.144 0.0 0.6 30.0 50.0 2.0 10.0 10.0 851.634 -H5 0.4 0.0 0.208 12.0 1.2 0.185 0.054 0.2 0.2 0.96 0.3 30.0 50.0 2.0 10.0 10.0 882.293 -I5 2.0 44.8 0.26 21.6 0.0 0.33 0.014 0.8 0.2 1.5 0.42 30.0 50.0 2.0 10.0 10.0 826.076 -J5 2.0 32.0 0.052 6.0 1.5 0.132 0.014 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1.5 0.211 0.068 1.0 0.08 0.3 0.0 30.0 50.0 2.0 10.0 10.0 894.233 -F6 0.4 51.2 0.26 30.0 1.2 0.185 0.054 0.4 0.2 0.3 0.0 30.0 50.0 2.0 10.0 10.0 813.8009999999999 -G6 0.4 51.2 0.0 12.0 0.96 0.211 0.038 0.64 0.112 0.3 0.0 30.0 50.0 2.0 10.0 10.0 832.139 -H6 1.2 44.8 0.0 16.8 0.84 0.33 0.014 0.8 0.2 0.6 0.15 30.0 50.0 2.0 10.0 10.0 832.266 -I6 2.0 64.0 0.052 12.0 0.84 0.0 0.014 0.2 0.04 1.2 0.42 30.0 50.0 2.0 10.0 10.0 817.2339999999999 -J6 2.0 0.0 0.0 6.0 1.2 0.066 0.038 1.0 0.128 0.6 0.6 30.0 50.0 2.0 10.0 10.0 886.3679999999999 -K6 1.2 44.8 0.146 21.6 1.08 0.066 0.049 0.64 0.04 1.5 0.6 30.0 50.0 2.0 10.0 10.0 826.279 -L6 1.2 44.8 0.166 6.0 0.3 0.066 0.0 0.4 0.08 0.6 0.15 30.0 50.0 2.0 10.0 10.0 844.238 -M6 0.4 16.0 0.146 24.0 0.6 0.0 0.068 0.4 0.16 1.5 0.42 30.0 50.0 2.0 10.0 10.0 854.306 -N6 2.0 64.0 0.104 0.0 0.3 0.33 0.0 0.2 0.144 0.3 0.0 30.0 50.0 2.0 10.0 10.0 830.6220000000001 -O6 1.2 32.0 0.208 6.0 0.84 0.132 0.054 0.72 0.16 0.6 0.0 30.0 50.0 2.0 10.0 10.0 856.086 -P6 4.0 44.8 0.146 16.8 0.0 0.132 0.054 1.0 0.04 0.84 0.3 30.0 50.0 2.0 10.0 10.0 829.8879999999999 -A7 4.0 32.0 0.052 6.0 0.3 0.185 0.038 0.4 0.08 0.84 0.6 30.0 50.0 2.0 10.0 10.0 853.505 -B7 2.0 16.0 0.208 30.0 1.5 0.066 0.044 0.56 0.04 0.3 0.15 30.0 50.0 2.0 10.0 10.0 847.1320000000001 -C7 0.4 16.0 0.187 21.6 0.6 0.066 0.014 0.4 0.2 0.0 0.42 30.0 50.0 2.0 10.0 10.0 858.113 -D7 4.0 51.2 0.104 6.0 1.2 0.238 0.0 0.2 0.04 0.0 0.42 30.0 50.0 2.0 10.0 10.0 834.598
--- a/test-data/plates_generator_destination_plate_1.test-2.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,101 +0,0 @@ -Well Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS Water -C3 0.4 44.8 0.0 21.6 0.6 0.0 0.049 0.2 0.112 1.2 0.15 30.0 50.0 2.0 10.0 10.0 828.889 -D3 4.0 32.0 0.104 6.0 0.3 0.264 0.014 0.56 0.08 0.3 0.6 30.0 50.0 2.0 10.0 10.0 853.778 -E3 4.0 0.0 0.146 0.0 0.0 0.132 0.027 0.8 0.04 0.6 0.75 30.0 50.0 2.0 10.0 10.0 891.505 -F3 0.0 32.0 0.052 6.0 1.08 0.132 0.054 0.0 0.144 1.5 0.6 30.0 50.0 2.0 10.0 10.0 856.438 -G3 2.0 64.0 0.0 12.0 1.5 0.066 0.044 0.64 0.16 0.3 0.3 30.0 50.0 2.0 10.0 10.0 816.99 -H3 0.4 80.0 0.0 30.0 0.84 0.066 0.038 0.4 0.0 1.2 0.48 30.0 50.0 2.0 10.0 10.0 784.576 -I3 2.0 51.2 0.208 12.0 0.3 0.066 0.038 0.56 0.08 1.2 0.3 30.0 50.0 2.0 10.0 10.0 830.048 -J3 2.0 32.0 0.104 24.0 1.5 0.33 0.014 0.8 0.08 0.3 0.15 30.0 50.0 2.0 10.0 10.0 836.722 -K3 0.0 44.8 0.208 21.6 1.2 0.238 0.038 0.2 0.0 0.96 0.3 30.0 50.0 2.0 10.0 10.0 828.456 -L3 2.0 16.0 0.208 24.0 0.84 0.264 0.014 1.0 0.04 0.84 0.75 30.0 50.0 2.0 10.0 10.0 852.044 -M3 1.2 57.6 0.0 24.0 0.96 0.132 0.027 0.2 0.112 0.3 0.3 30.0 50.0 2.0 10.0 10.0 813.169 -N3 1.2 16.0 0.166 21.6 0.3 0.066 0.068 0.4 0.144 0.6 0.3 30.0 50.0 2.0 10.0 10.0 857.156 -O3 1.2 16.0 0.26 19.2 0.96 0.066 0.044 0.4 0.08 1.2 0.15 30.0 50.0 2.0 10.0 10.0 858.44 -P3 0.4 80.0 0.104 30.0 0.84 0.33 0.068 0.8 0.112 1.08 0.0 30.0 50.0 2.0 10.0 10.0 784.2660000000001 -A4 4.0 32.0 0.104 24.0 1.2 0.0 0.038 0.2 0.112 0.3 0.54 30.0 50.0 2.0 10.0 10.0 835.506 -B4 4.0 64.0 0.104 24.0 1.08 0.185 0.049 0.72 0.08 0.3 0.42 30.0 50.0 2.0 10.0 10.0 803.062 -C4 1.2 0.0 0.208 0.0 1.08 0.238 0.049 0.56 0.2 0.96 0.75 30.0 50.0 2.0 10.0 10.0 892.755 -D4 2.0 16.0 0.208 6.0 0.84 0.211 0.038 0.2 0.128 1.2 0.6 30.0 50.0 2.0 10.0 10.0 870.575 -E4 4.0 64.0 0.146 16.8 0.96 0.132 0.049 0.4 0.0 0.84 0.42 30.0 50.0 2.0 10.0 10.0 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10.0 785.056 -A5 2.0 32.0 0.146 12.0 1.2 0.211 0.054 0.64 0.08 0.96 0.6 30.0 50.0 2.0 10.0 10.0 848.1089999999999 -B5 1.2 51.2 0.146 12.0 0.6 0.132 0.014 0.2 0.0 1.08 0.54 30.0 50.0 2.0 10.0 10.0 830.8879999999999 -C5 2.0 64.0 0.052 30.0 1.2 0.066 0.068 0.8 0.128 0.3 0.15 30.0 50.0 2.0 10.0 10.0 799.236 -D5 4.0 0.0 0.208 0.0 0.3 0.0 0.044 0.0 0.16 0.6 0.15 30.0 50.0 2.0 10.0 10.0 892.538 -E5 1.2 64.0 0.26 0.0 0.0 0.264 0.0 0.56 0.04 1.2 0.54 30.0 50.0 2.0 10.0 10.0 829.9359999999999 -F5 2.0 64.0 0.052 6.0 0.96 0.185 0.038 0.0 0.112 0.84 0.6 30.0 50.0 2.0 10.0 10.0 823.213 -G5 4.0 64.0 0.187 6.0 0.3 0.185 0.027 0.4 0.112 0.3 0.15 30.0 50.0 2.0 10.0 10.0 822.3389999999999 -H5 1.2 51.2 0.052 24.0 0.6 0.185 0.027 0.4 0.08 0.6 0.3 30.0 50.0 2.0 10.0 10.0 819.356 -I5 4.0 16.0 0.104 24.0 0.0 0.185 0.054 0.4 0.04 1.2 0.54 30.0 50.0 2.0 10.0 10.0 851.477 -J5 2.0 32.0 0.26 12.0 1.08 0.238 0.044 0.2 0.08 1.5 0.48 30.0 50.0 2.0 10.0 10.0 848.1179999999999 -K5 2.0 64.0 0.187 6.0 0.6 0.33 0.038 0.8 0.144 0.3 0.15 30.0 50.0 2.0 10.0 10.0 823.451 -L5 4.0 32.0 0.104 6.0 0.84 0.132 0.027 0.2 0.04 0.96 0.75 30.0 50.0 2.0 10.0 10.0 852.947 -M5 4.0 0.0 0.104 6.0 0.6 0.33 0.0 1.0 0.16 1.08 0.3 30.0 50.0 2.0 10.0 10.0 884.426 -N5 0.4 51.2 0.26 12.0 0.0 0.264 0.014 0.4 0.0 0.3 0.3 30.0 50.0 2.0 10.0 10.0 832.8620000000001 -O5 2.0 80.0 0.187 24.0 0.6 0.264 0.027 0.8 0.08 1.2 0.6 30.0 50.0 2.0 10.0 10.0 788.242 -P5 2.0 16.0 0.26 24.0 0.6 0.132 0.027 1.0 0.04 0.3 0.48 30.0 50.0 2.0 10.0 10.0 853.1610000000001 -A6 2.0 57.6 0.26 6.0 1.08 0.238 0.054 0.2 0.04 0.84 0.3 30.0 50.0 2.0 10.0 10.0 829.3879999999999 -B6 0.0 16.0 0.052 30.0 1.5 0.33 0.068 1.0 0.16 1.5 0.15 30.0 50.0 2.0 10.0 10.0 847.24 -C6 4.0 80.0 0.146 12.0 0.84 0.066 0.054 0.2 0.08 1.08 0.54 30.0 50.0 2.0 10.0 10.0 798.9939999999999 -D6 4.0 32.0 0.052 12.0 0.3 0.066 0.027 0.0 0.16 0.0 0.42 30.0 50.0 2.0 10.0 10.0 848.975 -E6 2.0 16.0 0.104 30.0 0.3 0.132 0.044 1.0 0.128 0.6 0.54 30.0 50.0 2.0 10.0 10.0 847.152 -F6 1.2 16.0 0.104 16.8 1.2 0.066 0.068 0.4 0.16 0.3 0.48 30.0 50.0 2.0 10.0 10.0 861.222 -G6 1.2 51.2 0.187 19.2 1.5 0.264 0.014 0.0 0.2 0.84 0.15 30.0 50.0 2.0 10.0 10.0 823.245 -H6 2.0 64.0 0.146 6.0 1.5 0.066 0.014 0.56 0.2 0.84 0.3 30.0 50.0 2.0 10.0 10.0 822.374 -I6 0.4 16.0 0.104 12.0 0.3 0.132 0.054 0.0 0.04 0.6 0.48 30.0 50.0 2.0 10.0 10.0 867.89 -J6 4.0 16.0 0.146 24.0 0.6 0.0 0.014 0.8 0.08 0.3 0.48 30.0 50.0 2.0 10.0 10.0 851.58 -K6 4.0 44.8 0.052 12.0 0.96 0.211 0.0 0.0 0.08 1.5 0.3 30.0 50.0 2.0 10.0 10.0 834.097 -L6 4.0 57.6 0.166 19.2 0.96 0.066 0.0 0.8 0.128 1.5 0.75 30.0 50.0 2.0 10.0 10.0 812.83 -M6 0.4 16.0 0.166 16.8 0.3 0.0 0.014 1.0 0.16 1.5 0.3 30.0 50.0 2.0 10.0 10.0 861.36 -N6 2.0 80.0 0.0 24.0 0.0 0.264 0.014 0.8 0.0 0.3 0.42 30.0 50.0 2.0 10.0 10.0 790.202 -O6 2.0 57.6 0.052 16.8 0.6 0.066 0.014 0.72 0.128 1.5 0.15 30.0 50.0 2.0 10.0 10.0 818.37 -P6 2.0 57.6 0.104 24.0 0.3 0.132 0.027 0.8 0.112 0.96 0.42 30.0 50.0 2.0 10.0 10.0 811.5450000000001 -A7 0.0 0.0 0.187 19.2 0.84 0.066 0.0 0.2 0.0 0.84 0.75 30.0 50.0 2.0 10.0 10.0 875.917 -B7 2.0 64.0 0.26 16.8 0.96 0.264 0.027 0.64 0.144 1.2 0.6 30.0 50.0 2.0 10.0 10.0 811.105 -C7 1.2 32.0 0.104 16.8 0.6 0.132 0.027 0.72 0.08 0.6 0.15 30.0 50.0 2.0 10.0 10.0 845.587 -D7 1.2 16.0 0.052 6.0 0.84 0.33 0.014 0.0 0.144 1.08 0.6 30.0 50.0 2.0 10.0 10.0 871.74 -E7 1.2 64.0 0.208 6.0 0.6 0.238 0.049 0.72 0.0 0.0 0.42 30.0 50.0 2.0 10.0 10.0 824.565 -F7 2.0 80.0 0.052 19.2 1.5 0.185 0.027 0.4 0.112 0.84 0.15 30.0 50.0 2.0 10.0 10.0 793.534 -G7 2.0 57.6 0.052 0.0 1.08 0.238 0.014 0.64 0.08 0.6 0.3 30.0 50.0 2.0 10.0 10.0 835.396 -H7 2.0 16.0 0.166 19.2 0.0 0.185 0.054 0.56 0.128 1.08 0.0 30.0 50.0 2.0 10.0 10.0 858.627 -I7 0.0 32.0 0.104 12.0 0.84 0.0 0.038 0.64 0.144 0.0 0.6 30.0 50.0 2.0 10.0 10.0 851.634 -J7 0.4 0.0 0.208 12.0 1.2 0.185 0.054 0.2 0.2 0.96 0.3 30.0 50.0 2.0 10.0 10.0 882.293 -K7 2.0 44.8 0.26 21.6 0.0 0.33 0.014 0.8 0.2 1.5 0.42 30.0 50.0 2.0 10.0 10.0 826.076 -L7 2.0 32.0 0.052 6.0 1.5 0.132 0.014 0.4 0.112 0.84 0.3 30.0 50.0 2.0 10.0 10.0 854.65 -M7 2.0 32.0 0.166 19.2 1.5 0.238 0.014 0.64 0.16 0.6 0.42 30.0 50.0 2.0 10.0 10.0 841.062 -N7 1.2 32.0 0.146 0.0 0.0 0.185 0.068 0.56 0.04 1.2 0.54 30.0 50.0 2.0 10.0 10.0 862.0609999999999 -O7 2.0 57.6 0.052 0.0 1.2 0.211 0.027 0.72 0.04 1.2 0.75 30.0 50.0 2.0 10.0 10.0 834.2 -P7 4.0 80.0 0.052 30.0 0.6 0.0 0.054 0.56 0.112 0.0 0.0 30.0 50.0 2.0 10.0 10.0 782.6220000000001 -A8 0.4 16.0 0.187 0.0 1.2 0.264 0.0 0.2 0.16 1.2 0.75 30.0 50.0 2.0 10.0 10.0 877.639 -B8 4.0 32.0 0.104 12.0 0.3 0.132 0.027 0.8 0.112 0.0 0.15 30.0 50.0 2.0 10.0 10.0 848.375 -C8 4.0 16.0 0.208 21.6 0.0 0.264 0.049 0.2 0.0 0.0 0.15 30.0 50.0 2.0 10.0 10.0 855.529 -D8 2.0 64.0 0.187 12.0 0.6 0.132 0.027 0.2 0.04 0.96 0.75 30.0 50.0 2.0 10.0 10.0 817.104 -E8 2.0 32.0 0.104 30.0 0.3 0.066 0.044 0.56 0.04 0.96 0.15 30.0 50.0 2.0 10.0 10.0 831.7760000000001 -F8 4.0 80.0 0.166 19.2 0.6 0.132 0.027 0.2 0.2 0.0 0.48 30.0 50.0 2.0 10.0 10.0 792.995 -G8 0.4 0.0 0.208 0.0 1.5 0.211 0.068 1.0 0.08 0.3 0.0 30.0 50.0 2.0 10.0 10.0 894.233 -H8 0.4 51.2 0.26 30.0 1.2 0.185 0.054 0.4 0.2 0.3 0.0 30.0 50.0 2.0 10.0 10.0 813.8009999999999 -I8 0.4 51.2 0.0 12.0 0.96 0.211 0.038 0.64 0.112 0.3 0.0 30.0 50.0 2.0 10.0 10.0 832.139 -J8 1.2 44.8 0.0 16.8 0.84 0.33 0.014 0.8 0.2 0.6 0.15 30.0 50.0 2.0 10.0 10.0 832.266 -K8 2.0 64.0 0.052 12.0 0.84 0.0 0.014 0.2 0.04 1.2 0.42 30.0 50.0 2.0 10.0 10.0 817.2339999999999 -L8 2.0 0.0 0.0 6.0 1.2 0.066 0.038 1.0 0.128 0.6 0.6 30.0 50.0 2.0 10.0 10.0 886.3679999999999 -M8 1.2 44.8 0.146 21.6 1.08 0.066 0.049 0.64 0.04 1.5 0.6 30.0 50.0 2.0 10.0 10.0 826.279 -N8 1.2 44.8 0.166 6.0 0.3 0.066 0.0 0.4 0.08 0.6 0.15 30.0 50.0 2.0 10.0 10.0 844.238 -O8 0.4 16.0 0.146 24.0 0.6 0.0 0.068 0.4 0.16 1.5 0.42 30.0 50.0 2.0 10.0 10.0 854.306 -P8 2.0 64.0 0.104 0.0 0.3 0.33 0.0 0.2 0.144 0.3 0.0 30.0 50.0 2.0 10.0 10.0 830.6220000000001 -A9 1.2 32.0 0.208 6.0 0.84 0.132 0.054 0.72 0.16 0.6 0.0 30.0 50.0 2.0 10.0 10.0 856.086 -B9 4.0 44.8 0.146 16.8 0.0 0.132 0.054 1.0 0.04 0.84 0.3 30.0 50.0 2.0 10.0 10.0 829.8879999999999 -C9 4.0 32.0 0.052 6.0 0.3 0.185 0.038 0.4 0.08 0.84 0.6 30.0 50.0 2.0 10.0 10.0 853.505 -D9 2.0 16.0 0.208 30.0 1.5 0.066 0.044 0.56 0.04 0.3 0.15 30.0 50.0 2.0 10.0 10.0 847.1320000000001 -E9 0.4 16.0 0.187 21.6 0.6 0.066 0.014 0.4 0.2 0.0 0.42 30.0 50.0 2.0 10.0 10.0 858.113 -F9 4.0 51.2 0.104 6.0 1.2 0.238 0.0 0.2 0.04 0.0 0.42 30.0 50.0 2.0 10.0 10.0 834.598
--- a/test-data/plates_generator_source_plate_1.test-1.json Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,26 +0,0 @@ -{ - "Dimensions": "16x24", - "deadVolume": 15000, - "Well capacity": 60000, - "Current well": "C2", - "Parameters": [ - "Spermidine", - "CAMP", - "Extract", - "CoA", - "HEPES", - "Folinic acid", - "k-glutamate", - "PEG", - "Mg-glutamate", - "Promoter", - "RBS", - "Amino acids", - "NTP", - "Water", - "NAD", - "3-PGA", - "tRNA" - ], - "Wells": "plates_generator_source_plate_1.tsv" -}
--- a/test-data/plates_generator_source_plate_1.test-1.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,19 +0,0 @@ -Well Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS Water -A1 60000.0 -B1 60000.0 -C1 60000.0 -D1 60000.0 -E1 60000.0 -F1 60000.0 -G1 60000.0 -H1 60000.0 -I1 60000.0 -J1 60000.0 -K1 60000.0 -L1 60000.0 -M1 60000.0 -N1 60000.0 -O1 60000.0 -P1 60000.0 -A2 60000.0 -B2 60000.0
--- a/test-data/plates_generator_source_plate_1.test-2.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,19 +0,0 @@ -Well Mg-glutamate k-glutamate CoA 3-PGA NTP NAD Folinic acid Spermidine tRNA Amino acids CAMP Extract HEPES PEG Promoter RBS Water -A2 60000.0 -B2 60000.0 -C2 60000.0 -D2 60000.0 -E2 60000.0 -F2 60000.0 -G2 60000.0 -H2 60000.0 -I2 60000.0 -J2 60000.0 -K2 60000.0 -L2 60000.0 -M2 60000.0 -N2 60000.0 -O2 2500.0 -P2 2500.0 -Q2 60000.0 -R2 60000.0
--- a/test-data/plates_generator_volumes_summary.test-1.tsv Wed Feb 14 15:25:58 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,18 +0,0 @@ -Factor Sample+deadVolumes -Mg-glutamate 60000 -k-glutamate 60000 -CoA 60000 -3-PGA 60000 -NTP 60000 -NAD 60000 -Folinic acid 60000 -Spermidine 60000 -tRNA 60000 -Amino acids 60000 -CAMP 60000 -Extract 60000 -HEPES 60000 -PEG 60000 -Promoter 60000 -RBS 60000 -Water 120000
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/sampling.test-1.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,4 @@ +Hela lysate,Access prot 50%,Reaction mix,RNA 1ug/uL +492.5,25.0,310.0,152.5 +970.0,165.0,257.5,112.5 +537.5,325.0,192.5,185.0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/source_plate.csv Wed Jul 24 08:18:58 2024 +0000 @@ -0,0 +1,6 @@ +Well,Access prot 50%,Hela lysate,RNA 1ug/uL,Reaction mix,Water +A1,0.0,22000.0,0.0,0.0,0.0 +B1,20515.0,0.0,0.0,0.0,0.0 +C1,0.0,0.0,0.0,20760.0,0.0 +D1,0.0,0.0,20450.0,0.0,0.0 +E1,0.0,0.0,0.0,0.0,34275.0