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author | trinity_ctat |
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date | Tue, 17 Jul 2018 11:51:50 -0400 |
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<tool id="ctat_star_fusion" name="ctat_star_fusion" version="1.0.1" profile="17.05"> <description>Fusion-finding Pipeline Using the STAR Aligner</description> <requirements> <requirement type="package" version="1.4.0">star-fusion</requirement> </requirements> <!-- Using command's detect_errors instead of below. <stdio> <exit_code range="1:" level="fatal" description="Error returned from pipeline" /> </stdio> <regex match="Must investigate error above." source="stderr" level="fatal" description="Unknown error encountered" /> --> <command detect_errors="default"> <![CDATA[ STAR-Fusion --genome_lib_dir "${genome_resource_lib.fields.path}" --left_fq "${left_input}" --right_fq "${right_input}" --output_dir subdir --STAR_SortedByCoordinate ]]> </command> <inputs> <param format="fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/> <param format="fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/> <param name="genome_resource_lib" type="select" label="Select a reference genome"> <options from_data_table="ctat_genome_resource_libs"> <filter type="sort_by" column="2" /> <validator type="no_options" message="No indexes are available" /> </options> </param> </inputs> <outputs> <data format="txt" name="starfusion_log" label="${tool.name} on ${on_string}: log" from_work_dir="subdir/Log.out"/> <data format="bam" name="aligned_bam" label="${tool.name} on ${on_string}: Aligned Bam" from_work_dir="subdir/std.STAR.bam"/> <data format="tabular" name="candidates" label="${tool.name} on ${on_string}: Fusion candidates" from_work_dir="subdir/star-fusion.fusion_predictions.abridged.tsv"/> <data format="tabular" name="chimeric_junc" label="${tool.name} on ${on_string}: Chimeric.out.junction" from_work_dir="subdir/std.Chimeric.out.junction"/> </outputs> <tests> <test> <param name="left_input" value="StarFusion/reads_1.fq.gz" /> <param name="right_input" value="StarFusion/reads_2.fq.gz" /> <!-- <param name="left_input" value="reads.left.simPE.fq" /> <param name="right_input" value="reads.right.simPE.fq" /> --> <!-- FIX - now that we added the CTAT ref lib path as a parameter, how do we find it for testing? <param name="genome_resource_lib.fields.path" value="?????" /> --> <!-- <output name="aligned_bam" file="SF_out_aligned.bam" /> <output name="candidates" file="SF_out_fusion_candidates.dat" /> <output name="chimeric_junc" file="SF_out_chimeric.junction" /> <output name="starfusion_log" file="SF_out.log" /> --> <output name="starfusion_log"> <assert_contents> <has_line_matching expression=".+" /> <has_line line="ALL DONE!" /> </assert_contents> </output> <output name="aligned_bam"> <assert_contents> <has_line_matching expression=".+" /> <!-- The following checks for the magic number at the start of the bam file --> <!-- At first I thought "\x8B\x1F" was the number, but it turns out the file produced by the StarFusion test had that sequence in the file somewhere else. --> <has_text_matching expression="\x1F\x8B" /> </assert_contents> </output> <output name="candidates"> <assert_contents> <has_line_matching expression=".+" /> <has_line line="#fusion_name	JunctionReads	SpanningFrags	Splice_type	LeftGene	LeftBreakpoint	RightGene	RightBreakpoint	Long_double_anchor_support" /> </assert_contents> </output> <output name="chimeric_junc"> <assert_contents> <has_line_matching expression=".+" /> <has_line_matching expression="^chr20.*" /> </assert_contents> </output> </test> </tests> <help> .. class:: infomark STAR-Fusion is a component of the Trinity Cancer Transcriptome Analysis Toolkit (CTAT). STAR-Fusion uses the STAR aligner to identify candidate fusion transcripts supported by Illumina reads. STAR-Fusion further processes the output generated by the STAR aligner to map junction reads and spanning reads to a reference annotation set. Please read more here_. .. _here: https://github.com/STAR-Fusion/STAR-Fusion/wiki </help> </tool>