changeset 0:46ddb0591d8b draft default tip

planemo upload commit a2411926bebc2ca3bb31215899a9f18a67e59556
author vmarcon
date Thu, 18 Jan 2018 07:44:37 -0500
parents
children
files LICENSE static/images/ChangeDatatype.pdf static/images/descriptive_stat_all.png static/images/descriptive_stat_boxplot.png static/images/descriptive_stat_density.png static/images/descriptive_stat_histo.png static/images/descriptive_stat_maplot.png static/images/descriptive_stat_pairs.png static/images/input_count_file.png summary_statistics.R summary_statistics.xml summary_statistics_galaxy.R test-data/decathlon.tsv test-data/graph_file test-data/log_file test-data/table_file
diffstat 16 files changed, 1324 insertions(+), 0 deletions(-) [+]
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/LICENSE	Thu Jan 18 07:44:37 2018 -0500
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+author or copyright holder as a result of your choosing to follow a
+later version.
+
+  15. Disclaimer of Warranty.
+
+  THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY
+APPLICABLE LAW.  EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT
+HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY
+OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO,
+THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
+PURPOSE.  THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM
+IS WITH YOU.  SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF
+ALL NECESSARY SERVICING, REPAIR OR CORRECTION.
+
+  16. Limitation of Liability.
+
+  IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING
+WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MODIFIES AND/OR CONVEYS
+THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY
+GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE
+USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF
+DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD
+PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS),
+EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF
+SUCH DAMAGES.
+
+  17. Interpretation of Sections 15 and 16.
+
+  If the disclaimer of warranty and limitation of liability provided
+above cannot be given local legal effect according to their terms,
+reviewing courts shall apply local law that most closely approximates
+an absolute waiver of all civil liability in connection with the
+Program, unless a warranty or assumption of liability accompanies a
+copy of the Program in return for a fee.
+
+                     END OF TERMS AND CONDITIONS
+
+            How to Apply These Terms to Your New Programs
+
+  If you develop a new program, and you want it to be of the greatest
+possible use to the public, the best way to achieve this is to make it
+free software which everyone can redistribute and change under these terms.
+
+  To do so, attach the following notices to the program.  It is safest
+to attach them to the start of each source file to most effectively
+state the exclusion of warranty; and each file should have at least
+the "copyright" line and a pointer to where the full notice is found.
+
+    {one line to give the program's name and a brief idea of what it does.}
+    Copyright (C) {year}  {name of author}
+
+    This program is free software: you can redistribute it and/or modify
+    it under the terms of the GNU General Public License as published by
+    the Free Software Foundation, either version 3 of the License, or
+    (at your option) any later version.
+
+    This program is distributed in the hope that it will be useful,
+    but WITHOUT ANY WARRANTY; without even the implied warranty of
+    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+    GNU General Public License for more details.
+
+    You should have received a copy of the GNU General Public License
+    along with this program.  If not, see <http://www.gnu.org/licenses/>.
+
+Also add information on how to contact you by electronic and paper mail.
+
+  If the program does terminal interaction, make it output a short
+notice like this when it starts in an interactive mode:
+
+    {project}  Copyright (C) {year}  {fullname}
+    This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'.
+    This is free software, and you are welcome to redistribute it
+    under certain conditions; type `show c' for details.
+
+The hypothetical commands `show w' and `show c' should show the appropriate
+parts of the General Public License.  Of course, your program's commands
+might be different; for a GUI interface, you would use an "about box".
+
+  You should also get your employer (if you work as a programmer) or school,
+if any, to sign a "copyright disclaimer" for the program, if necessary.
+For more information on this, and how to apply and follow the GNU GPL, see
+<http://www.gnu.org/licenses/>.
+
+  The GNU General Public License does not permit incorporating your program
+into proprietary programs.  If your program is a subroutine library, you
+may consider it more useful to permit linking proprietary applications with
+the library.  If this is what you want to do, use the GNU Lesser General
+Public License instead of this License.  But first, please read
+<http://www.gnu.org/philosophy/why-not-lgpl.html>.
Binary file static/images/ChangeDatatype.pdf has changed
Binary file static/images/descriptive_stat_all.png has changed
Binary file static/images/descriptive_stat_boxplot.png has changed
Binary file static/images/descriptive_stat_density.png has changed
Binary file static/images/descriptive_stat_histo.png has changed
Binary file static/images/descriptive_stat_maplot.png has changed
Binary file static/images/descriptive_stat_pairs.png has changed
Binary file static/images/input_count_file.png has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/summary_statistics.R	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,263 @@
+###########################################################################
+#            Quality controls and descriptive analysis plots              #
+###########################################################################
+# Authors: Melanie Petera                                                 #
+###########################################################################
+# Description : This script allows various displays of data for quality   #
+#      control and descriptive analysis. The input data is a matrix of    #
+#      quantitative variables, and it returns chosen plots in png format  #
+#      and a table with chosen statistics.                                #
+###########################################################################
+# Specific R packages:                                                    #
+#      - edgeR (needed for MA plots)                                      #
+###########################################################################
+# Version 1 (06-06-2014): display boxplot, histogram, density plot,       #
+#      MA plot, pairs plot, and return a table of chosen statistics       #
+#      (quantiles, mean, variance, standard error of the mean)            #
+###########################################################################
+        
+desc_fct <- function(file.in, nacode, table_file, graph_file, stat, chosen.stat, ploting, chosen.plot, log_file){
+  # Parameters:
+  # - file.in: count matrix input (tab-separated) [file name]
+  # - nacode: missing value coding character
+  # - table_file: results file containing table of chosen statistics [file name]
+  # - graph_file: pdf file containing plots for chosen statistics [file name]
+  # - stat: should statistics be calculated? (TRUE/FALSE)
+  # - chosen.stat: character listing the chosen statistics (comma-separated)
+  # - ploting: should graphics be displayed? (TRUE/FALSE)
+  # - chosen.plot: character listing the chosen plots (comma-separated)
+  # - log_file: a log file [file name]
+
+
+##########################################################
+# Read and verify data - - - - - - - - - - - - 
+# Checks valids for all modules
+
+library(methods)
+
+log_error=function(message="") {
+	line_use="line"
+	column_use="column"
+
+	cat("<HTML><HEAD><TITLE>Normalization report</TITLE></HEAD><BODY>\n",file=log_file,append=F,sep="")
+	cat("&#9888 An error occurred while trying to read your table.\n<BR>",file=log_file,append=T,sep="")
+	cat("Please check that:\n<BR>",file=log_file,append=T,sep="")
+	cat("<UL>\n",file=log_file,append=T,sep="")
+	cat("  <LI> the table you want to process contains the same number of columns for each line</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> the first line of your table is a header line (specifying the name of each ",column_use,")</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> the first column of your table specifies the name of each ",line_use,"</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> both individual and variable names should be unique</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> each value is separated from the other by a <B>TAB</B> character</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> except for first line and first column, table should contain a numeric value</LI>\n",file=log_file,append=T,sep="")
+	cat("  <LI> this value may contain character '.' as decimal separator or '",nacode,"' for missing values</LI>\n",file=log_file,append=T,sep="")
+	cat("</UL>\n",file=log_file,append=T,sep="")
+	cat("-------<BR>\nError messages recieved:<BR><FONT color=red>\n",conditionMessage(message),"</FONT>\n",file=log_file,append=T,sep="")
+	cat("</BODY></HTML>\n",file=log_file,append=T,sep="")
+	q(save="no",status=1)
+}
+
+tab_in=tryCatch(
+	{
+		tab_in=read.table(file.in,header=TRUE,na.strings=nacode,sep="\t",check.names=FALSE,quote="\"")
+	},
+	error=function(cond) {
+		log_error(message=cond)
+		return(NA)
+	},
+	warning=function(cond) {
+		log_error(message=cond)
+		return(NA)
+	},
+	finally={
+		#Do nothing special
+	}
+)
+
+if (ncol(tab_in)<2) {
+	log_error(simpleCondition("The table you want to use contains less than two columns."))
+}
+
+rn=as.character(tab_in[,1])
+if (length(rn)!=length(unique(rn))) {
+	duplicated_rownames=table(rn)
+	duplicated_rownames=duplicated_rownames[duplicated_rownames>1]
+	duplicated_rownames=names(duplicated_rownames)
+	if (length(duplicated_rownames)>3) {
+		duplicated_rownames=c(duplicated_rownames[1:3],"...")
+	}
+	duplicated_rownames=paste(duplicated_rownames,collapse=", ")
+	log_error(simpleCondition(
+		paste("The table you want to use have duplicated values in the first column (",
+			" - duplicated names: ",duplicated_rownames,sep="")
+	))
+}
+tab=tab_in[,-1]
+rownames(tab)=rn
+
+#Check all columns are numerical
+tab=as.matrix(tab)
+cell.with.na=c()
+for (i in 1:ncol(tab)) {
+	na.v1=is.na(tab[,i])
+	na.v2=is.na(as.numeric(tab[,i]))
+	if (sum(na.v1)!=sum(na.v2)) {
+		sel=which(na.v1!=na.v2)
+		sel=sel[1]
+		value=tab[sel,i]
+		log_error(simpleCondition(
+			paste("Column '",colnames(tab)[i],"' of your table contains non numerical values. Please check its content (on line #",sel,": value='",value,"').",sep="")
+		))
+	}
+	if (length(cell.with.na)==0 & sum(na.v1)!=0) {
+		cell.with.na=c(i,which(na.v1)[1])
+	}
+}
+
+Dataset <- tab_in
+
+##########################################################
+# Statistics table computation - - - - - - - - -
+
+log=""
+
+if(stat=="T" & length(chosen.stat)!=0){
+  
+  stat.list <- strsplit(chosen.stat,",")[[1]]
+  stat.res <- t(Dataset[0,,drop=FALSE])
+  
+  numdig <- 5
+  
+  if("mean" %in% stat.list){  
+    stat.res <- cbind(stat.res,c("Mean",round(colMeans(Dataset[,-1],na.rm=TRUE),digits=numdig)))
+  }
+  
+  if("sd" %in% stat.list){
+    colSd <- apply(Dataset[,-1],2,sd,na.rm=TRUE)
+    stat.res <- cbind(stat.res,c("Std.Dev",round(colSd,digits=numdig)))
+  } 
+  
+  if("variance" %in% stat.list){
+    colVar <- apply(Dataset[,-1],2,var,na.rm=TRUE)
+    stat.res <- cbind(stat.res,c("Variance",round(colVar,digits=numdig)))
+  }
+  
+  if(("median" %in% stat.list)&&(!("quartile" %in% stat.list))){
+    colMed <- apply(Dataset[,-1],2,median,na.rm=TRUE)
+    stat.res <- cbind(stat.res,c("Median",round(colMed,digits=numdig)))
+  }
+  
+  if("quartile" %in% stat.list){
+    colQ <- round(apply(Dataset[,-1],2,quantile,na.rm=TRUE),digits=numdig)
+    stat.res <- cbind(stat.res,c("Min",colQ[1,]),c("Q1",colQ[2,]),
+                      c("Median",colQ[3,]),c("Q3",colQ[4,]),c("Max",colQ[5,]))
+  }
+  
+  if("decile" %in% stat.list){
+    colD <- round(t(apply(Dataset[,-1],2,quantile,na.rm=TRUE,seq(0,1,0.1))),digits=numdig)
+    colD <- rbind(paste("D",seq(0,10,1),sep=""),colD)
+    stat.res <- cbind(stat.res,colD)
+  }
+  
+  write.table(stat.res,table_file,col.names=FALSE,sep="\t",quote=FALSE)
+
+  log=paste(log,"&#10132; You choose to compute :",chosen.stat,"<BR>")
+ 
+} # end if(stat)
+else{
+  log=paste(log,"&#10132; You don't choose any stats<BR>")
+}
+
+##########################################################
+# Graphics generation - - - - - - - - - - - - - 
+
+if(ploting=="T" & length(chosen.plot)!=0){
+  
+  nb_graph_per_row=4
+  nb_graph=ncol(Dataset)-1
+
+  nb_row=round(nb_graph/nb_graph_per_row)
+
+  nb_empty_plot=nb_graph %% nb_graph_per_row
+  if (nb_empty_plot != 0) {
+	nb_row=nb_row+1
+  }
+
+  page_height=3.5 * nb_row
+	
+  pdf(file=graph_file,height=page_height)
+
+  graph.list <- strsplit(chosen.plot,",")[[1]]
+
+  #For the pair plot, we stick to the default layout
+  if("pairsplot" %in% graph.list){
+    pairs(Dataset[,-1])
+  }
+
+  #For the other plots, we have 4 plots per line
+  par(mfrow=c(nb_row,nb_graph_per_row),mar=c(3, 3, 3, 1) + 0.1)
+  
+  if("boxplot" %in% graph.list){
+    for(ech in 2:ncol(Dataset)){
+      boxplot(Dataset[,ech],main=colnames(Dataset)[ech],xlab=NULL)
+    }
+    #Complete page with empty plots
+    i=0; while (i<nb_empty_plot) {plot.new();i=i+1;}
+  }
+  
+  if("histogram" %in% graph.list){
+    for(ech in 2:ncol(Dataset)){
+      hist(Dataset[,ech],main=colnames(Dataset)[ech],xlab=NULL)
+    }
+    #Complete page with empty plots
+    i=0; while (i<nb_empty_plot) {plot.new();i=i+1;}
+  }
+  
+  if("density" %in% graph.list){
+    for(ech in 2:ncol(Dataset)){
+      plot(density(Dataset[,ech],na.rm=TRUE),main=colnames(Dataset)[ech])
+    }
+    #Complete page with empty plots
+    i=0; while (i<nb_empty_plot) {plot.new();i=i+1;}
+  }
+  
+  
+  if("MAplot" %in% graph.list){
+    if(min(Dataset[,-1],na.rm=TRUE)<0){
+	  cat("\n----\nError: MAplot only available for positive variables\n----",file=log_file,append=T,sep="")
+	  q(save="no",status=1)
+	}
+    library(limma)
+
+    library(edgeR) #Warning : Import also limma package
+    for(ech in 2:(ncol(Dataset)-1)){
+      for(ech2 in (ech+1):ncol(Dataset)){
+        temp.pair <- na.omit(Dataset[,c(ech,ech2)])
+        maPlot(temp.pair[,1],temp.pair[,2],main=paste(colnames(Dataset)[ech],"VS",colnames(Dataset)[ech2]))
+      }
+    }
+    #Do not complete page with empty plots for this plot because it generates nb_variables X nb_variables graphs
+  }
+
+  #Close pdf device
+  dev.off()
+  
+  log=paste(log,"&#10132; You choose to plot :",chosen.plot,"<BR>")
+} # end if(ploting)
+else{
+  log=paste(log,"&#10132; You don't choose any plot<BR>")
+}
+
+
+
+##########################################################
+# Treatment successfull
+##########################################################
+cat("<HTML><HEAD><TITLE>Summary statistics report</TITLE></HEAD><BODY>\n",file=log_file,append=F,sep="")
+cat(log,file=log_file,append=T,sep="")
+cat("&#10003; Your process is successfull!<BR>",file=log_file,append=T,sep="")
+cat("</BODY></HTML>\n",file=log_file,append=T,sep="")
+
+
+} # end of function
+
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/summary_statistics.xml	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,280 @@
+<!--# Copyright (C) 2017 INRA
+# This program is free software: you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation, either version 3 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+# 
+# You should have received a copy of the GNU General Public License
+# along with this program.  If not, see http://www.gnu.org/licenses/.
+#-->
+
+<tool id="summary_statistics" name="Summary statistics" version="1.0.0">
+    <description>Produce simple descriptive statistics from a numerical table</description>
+    <requirements>
+        <requirement type="package">R</requirement>
+        <requirement type="package">bioconductor-edger</requirement>
+        <requirement type="package">bioconductor-limma</requirement>
+        <requirement type="package">r-batch</requirement>
+        <requirement type="package">r-locfit</requirement>
+    </requirements>
+    <stdio>
+        <!-- Anything other than zero is an error -->
+        <exit_code range="1:" level="fatal" />
+        <exit_code range=":-1" level="fatal" />
+    </stdio>
+    <command interpreter="Rscript"><![CDATA[
+        summary_statistics_galaxy.R
+        file_in '${file_in}'
+        NA_code '${NA_code}'
+        stat '${stat_cond.stat}'
+        #if $stat_cond.stat =="T":
+          stat_chosen '${stat_cond.stat_chosen}'
+        #end if
+        ploting  '${ploting_cond.ploting}'
+        #if $ploting_cond.ploting =="T":
+          plot_chosen '${ploting_cond.plot_chosen}'
+        #end if
+        table_file '${table_file}'
+        graph_file '${graph_file}'
+        log_file '${log_file}'
+    ]]></command>
+    <inputs>
+        <param format="csv,tabular" name="file_in" type="data" label="Input File" />
+        <param name="NA_code" size="30" type="text" value="NA" label="Label used for Missing values" />
+        <conditional name="stat_cond">
+            <param name="stat" type="select" help="Do you want to compute some basic statistics?" label="Statistics table">
+                <option value="T">Yes</option>
+                <option value="F" selected="true">No</option>
+            </param>
+            <when value="T">
+                <param name="stat_chosen" type="select" display="checkboxes" multiple="True" label="Chosen statistic(s)">
+                    <option value="mean">mean</option>
+                    <option value="sd">sd</option>
+                    <option value="variance">variance</option>
+                    <option value="median">median</option>
+                    <option value="quartile">quartile</option>
+                    <option value="decile">decile</option>
+                    <validator type="empty_field" message="Please choose at least one statistic representation" />
+                </param>
+            </when>
+        </conditional>
+        <conditional name="ploting_cond">
+            <param name="ploting" type="select" help="Do you want some standard plots?" label="Plots">
+                <option value="T">Yes</option>
+                <option value="F" selected="true">No</option>
+            </param>
+            <when value="T">
+                <param name="plot_chosen" type="select" help="" display="checkboxes" multiple="True" label="Chosen plot(s)">
+                    <option value="boxplot">boxplot</option>
+                    <option value="histogram">histogram</option>
+                    <option value="density">density</option>
+                    <option value="pairsplot">pairsplot</option>
+                    <option value="MAplot">MAplot</option>
+                    <validator type="empty_field" message="Please choose at least one ploting representation." />
+                </param>
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="html" name="log_file" label="Summary_statistics_log" />
+        <data format="tabular" name="table_file" label="Summary_statistics_report.tsv" >
+            <filter>(stat_cond['stat'] == 'T')</filter>
+        </data>
+        <data format="pdf" name="graph_file" label="Summary_statistics_report.pdf" >
+            <filter>(ploting_cond['ploting'] == 'T')</filter>
+        </data>
+    </outputs>
+    <tests>
+        <test>
+            <param name="file_in" value="decathlon.tsv"/>
+            <param name="Na_code" value="NA"/>
+            <conditional name="stat_cond">
+                <param name="stat" value="T"/>
+                <param name="stat_chosen" value="mean,sd,variance,median,quartile,decile"/>
+            </conditional>
+            <conditional name="ploting_cond">
+                <param name="ploting" value="T"/>
+                <param name="plot_chosen" value="boxplot,histogram,density,pairsplot,MAplot"/>
+            </conditional>
+            <output name="log_file" file="log_file"/>
+            <output name="table_file" file="table_file"/>
+            <output name="graph_file" file="graph_file" compare="sim_size"/>
+        </test>
+    </tests>
+    <help><![CDATA[
+
+==================
+Summary statistics
+==================
+
+-----------
+Description
+-----------
+
+ - This tool is part of a set of statistical tools made by members of the BIOS4BIOL group ("Normalization", "Summary statistics", "Hierarchical clustering" and "PCAFactoMineR").
+ - Please use the Normalization module that come with the suite before using this module.
+
+
+What it does:
+ - This program produces simple descriptive statistics from a numerical table. Statistical measures are computed for each column in the table.
+
+------
+
+-----------
+Input files
+-----------
+
++---------------------------+------------+
+| Parameter : num + label   |   Format   |
++===========================+============+
+| 1 : input file            |   tabular  |
++---------------------------+------------+
+
+The input table should be a tabulation-separated file. If your dataset is available in your current history but can not be selected via the Input File drop-down list, it may be due to incorrect format.
+Please first check that your data are effectively tabulated. If it is, then you can redefine the Galaxy format of your table into "tabular" editing its attributes ("Datatype" panel) [`See the schematic explanation here`_]. 
+
+
+.. _See the schematic explanation here: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/ChangeDatatype.pdf
+
+The first line should be a header (naming columns) and each line must begin with a row name, like the example below: 
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/input_count_file.png
+
+
+
+----------
+Parameters
+----------
+
+Label used for Missing values
+        | Missing value coding characters
+        |
+
+statistics table
+        | if YES, allow you to choose statistic(s) you want in your report
+        |
+
+Chosen statistic(s)
+        | select the statistics you want in your report (see above "Available statistics and plots")
+        |
+
+Plots
+        | if YES, allow you to choose plot(s) you want in your report
+        |
+Chosen plot(s)
+        | select the plots you want in your report (see above "Available statistics and plots")
+        |
+
+------------------------------
+Available statistics and plots
+------------------------------
+
+**Numerical statistical measures provided are the following ones:**
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_all.png
+        :width: 500
+
+
+**Available plots:**
+
+
+	* boxplot
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_boxplot.png
+
+(source : SAS documentation)
+
+	* histogram
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_histo.png
+        :width: 285
+
+In this example, about 45 values of the dataset are greater than 0 and lower than 0.5
+
+	* density
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_density.png
+        :width: 275
+
+This option computes kernel density estimates (gaussian smoothing).
+While a histogram displays the observed distribution of a numerical variable, a density plot allows to view the estimated distribution of the theoretical continuous variable. 
+
+	* pairsplot
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_pairs.png
+        :width: 475
+
+In this example, we have represented a pairs plot for a table with three columns, named 
+"a", "b" and "c". Each plot represents the values of a given column scaled on the x axis versus
+the values of another column scaled on the y axis.
+
+	* MAplot
+
+
+.. image:: https://raw.githubusercontent.com/IFB-ElixirFr/GFLS/master/summary_statistics/static/images/descriptive_stat_maplot.png
+        :width: 275
+
+Designed for genomic data (count data - only positive values accepted).
+Each plot allows to compare two samples. 
+The ordinate axis (M) represents the log ratios (binary logarithm) whereas the abscissa axis (A) corresponds to the means of log values. 
+
+
+
+------------
+Output files
+------------
+
+Summary_statistics_report.tsv
+	| contains a table with all the requested statistics
+        |
+ 
+Summary_statistics_report.pdf
+	| contains all the requested graphics
+        | 
+
+Summary_statistics_log
+        |
+        |
+ 
+------
+
+**Authors** Luc Jouneau (luc.jouneau@inra.fr), Mélanie Pétéra (melanie.petera@inra.fr), Sarah Maman (sarah.maman@inra.fr) and Valentin Marcon (valentin.marcon@inra.fr)
+
+Contact : support.sigenae@inra.fr
+
+E-learning available : Not yet.
+
+- Information :
+
+Tool coded in the R language:
+	| *R Core Team. R: A language and environment for statistical computing. R*
+	| *Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/.*
+
+.. class:: infomark
+
+-------------
+Please cite :
+-------------
+
+- (Depending on the help provided you can cite us in acknowledgements, references or both.)
+    
+Acknowledgements
+        | We wish to thank SIGENAE group and the statistical CATI BIOS4Biol group : Mélanie Pétéra, Sarah Maman, Luc Jouneau
+        | Re-packaging was provided by Valentin Marcon (INRA, Migale platform http://migale.jouy.inra.fr), as part of the IFB project 'Galaxy For Life Science' (http://www.france-bioinformatique.fr/fr)
+        |   
+ 
+References
+        | SIGENAE [http://www.sigenae.org/]
+
+    ]]></help>
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/summary_statistics_galaxy.R	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,52 @@
+###########################################################################
+#            Quality controls and descriptive analysis plots              #
+###########################################################################
+# Authors: Melanie Petera                                                 #
+###########################################################################
+# Description : This script allows various displays of data for quality   #
+#      control and descriptive analysis. The input data is a matrix of    #
+#      quantitative variables, and it returns chosen plots in png format  #
+#      and a table with chosen statistics.                                #
+###########################################################################
+# Specific R packages:                                                    #
+#      - edgeR (needed for MA plots)                                      #
+###########################################################################
+# Version 1 (06-06-2014): display boxplot, histogram, density plot,       #
+#      MA plot, pairs plot, and return a table of chosen statistics       #
+#      (quantiles, mean, variance, standard error of the mean)            #
+###########################################################################
+
+
+##------------------------------
+## Libraries laoding
+##------------------------------
+# For parseCommandArgs function
+library(batch) 
+
+# R script call
+source_local <- function(fname)
+{
+	argv <- commandArgs(trailingOnly = FALSE)
+	base_dir <- dirname(substring(argv[grep("--file=", argv)], 8))
+	source(paste(base_dir, fname, sep="/"))
+}
+
+#Import the different functions used for Summary_Statistics
+source_local("summary_statistics.R")
+
+##------------------------------
+## Lecture parametres
+##------------------------------
+argLs <- parseCommandArgs(evaluate=FALSE)
+
+desc_fct(file.in=argLs[["file_in"]],
+nacode=argLs[["NA_code"]],
+table_file=argLs[["table_file"]],
+graph_file=argLs[["graph_file"]],
+stat=argLs[["stat"]],
+chosen.stat=argLs[["stat_chosen"]],
+ploting=argLs[["ploting"]],
+chosen.plot=argLs[["plot_chosen"]],
+log_file=argLs[["log_file"]])
+
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/decathlon.tsv	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,42 @@
+"name"	"100m"	"Long.jump"	"Shot.put"	"High.jump"	"400m"	"110m.hurdle"	"Discus"	"Pole.vault"	"Javeline"	"1500m"
+"SEBRLE"	11.04	7.58	14.83	2.07	49.81	14.69	43.75	5.02	63.19	291.7
+"CLAY"	10.76	7.4	14.26	1.86	49.37	14.05	50.72	4.92	60.15	301.5
+"KARPOV"	11.02	7.3	14.77	2.04	48.37	14.09	48.95	4.92	50.31	300.2
+"BERNARD"	11.02	7.23	14.25	1.92	48.93	14.99	40.87	5.32	62.77	280.1
+"YURKOV"	11.34	7.09	15.19	2.1	50.42	15.31	46.26	4.72	63.44	276.4
+"WARNERS"	11.11	7.6	14.31	1.98	48.68	14.23	41.1	4.92	51.77	278.1
+"ZSIVOCZKY"	11.13	7.3	13.48	2.01	48.62	14.17	45.67	4.42	55.37	268
+"McMULLEN"	10.83	7.31	13.76	2.13	49.91	14.38	44.41	4.42	56.37	285.1
+"MARTINEAU"	11.64	6.81	14.57	1.95	50.14	14.93	47.6	4.92	52.33	262.1
+"HERNU"	11.37	7.56	14.41	1.86	51.1	15.06	44.99	4.82	57.19	285.1
+"BARRAS"	11.33	6.97	14.09	1.95	49.48	14.48	42.1	4.72	55.4	282
+"NOOL"	11.33	7.27	12.68	1.98	49.2	15.29	37.92	4.62	57.44	266.6
+"BOURGUIGNON"	11.36	6.8	13.46	1.86	51.16	15.67	40.49	5.02	54.68	291.7
+"Sebrle"	10.85	7.84	16.36	2.12	48.36	14.05	48.72	5	70.52	280.01
+"Clay"	10.44	7.96	15.23	2.06	49.19	14.13	50.11	4.9	69.71	282
+"Karpov"	10.5	7.81	15.93	2.09	46.81	13.97	51.65	4.6	55.54	278.11
+"Macey"	10.89	7.47	15.73	2.15	48.97	14.56	48.34	4.4	58.46	265.42
+"Warners"	10.62	7.74	14.48	1.97	47.97	14.01	43.73	4.9	55.39	278.05
+"Zsivoczky"	10.91	7.14	15.31	2.12	49.4	14.95	45.62	4.7	63.45	269.54
+"Hernu"	10.97	7.19	14.65	2.03	48.73	14.25	44.72	4.8	57.76	264.35
+"Nool"	10.8	7.53	14.26	1.88	48.81	14.8	42.05	5.4	61.33	276.33
+"Bernard"	10.69	7.48	14.8	2.12	49.13	14.17	44.75	4.4	55.27	276.31
+"Schwarzl"	10.98	7.49	14.01	1.94	49.76	14.25	42.43	5.1	56.32	273.56
+"Pogorelov"	10.95	7.31	15.1	2.06	50.79	14.21	44.6	5	53.45	287.63
+"Schoenbeck"	10.9	7.3	14.77	1.88	50.3	14.34	44.41	5	60.89	278.82
+"Barras"	11.14	6.99	14.91	1.94	49.41	14.37	44.83	4.6	64.55	267.09
+"Smith"	10.85	6.81	15.24	1.91	49.27	14.01	49.02	4.2	61.52	272.74
+"Averyanov"	10.55	7.34	14.44	1.94	49.72	14.39	39.88	4.8	54.51	271.02
+"Ojaniemi"	10.68	7.5	14.97	1.94	49.12	15.01	40.35	4.6	59.26	275.71
+"Smirnov"	10.89	7.07	13.88	1.94	49.11	14.77	42.47	4.7	60.88	263.31
+"Qi"	11.06	7.34	13.55	1.97	49.65	14.78	45.13	4.5	60.79	272.63
+"Drews"	10.87	7.38	13.07	1.88	48.51	14.01	40.11	5	51.53	274.21
+"Parkhomenko"	11.14	6.61	15.69	2.03	51.04	14.88	41.9	4.8	65.82	277.94
+"Terek"	10.92	6.94	15.15	1.94	49.56	15.12	45.62	5.3	50.62	290.36
+"Gomez"	11.08	7.26	14.57	1.85	48.61	14.41	40.95	4.4	60.71	269.7
+"Turi"	11.08	6.91	13.62	2.03	51.67	14.26	39.83	4.8	59.34	290.01
+"Lorenzo"	11.1	7.03	13.22	1.85	49.34	15.38	40.22	4.5	58.36	263.08
+"Karlivans"	11.33	7.26	13.3	1.97	50.54	14.98	43.34	4.5	52.92	278.67
+"Korkizoglou"	10.86	7.07	14.81	1.94	51.16	14.96	46.07	4.7	53.05	317
+"Uldal"	11.23	6.99	13.53	1.85	50.95	15.09	43.01	4.5	60	281.7
+"Casarsa"	11.36	6.68	14.92	1.94	53.2	15.39	48.66	4.4	58.62	296.12
Binary file test-data/graph_file has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/log_file	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,2 @@
+<HTML><HEAD><TITLE>Summary statistics report</TITLE></HEAD><BODY>
+ &#10132; You choose to compute : mean,sd,variance,median,quartile,decile <BR> &#10132; You choose to plot : boxplot,histogram,density,pairsplot,MAplot <BR>&#10003; Your process is successfull!<BR></BODY></HTML>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/table_file	Thu Jan 18 07:44:37 2018 -0500
@@ -0,0 +1,11 @@
+name	Mean	Std.Dev	Variance	Min	Q1	Median	Q3	Max	D0	D1	D2	D3	D4	D5	D6	D7	D8	D9	D10
+100m	10.99805	0.26302	0.06918	10.44	10.85	10.98	11.14	11.64	10.44	10.68	10.83	10.87	10.91	10.98	11.06	11.11	11.23	11.34	11.64
+Long.jump	7.26	0.3164	0.10011	6.61	7.03	7.3	7.48	7.96	6.61	6.81	6.99	7.07	7.23	7.3	7.31	7.4	7.5	7.6	7.96
+Shot.put	14.47707	0.82443	0.67968	12.68	13.88	14.57	14.97	16.36	12.68	13.46	13.62	14.09	14.31	14.57	14.77	14.91	15.15	15.31	16.36
+High.jump	1.97683	0.08895	0.00791	1.85	1.92	1.95	2.04	2.15	1.85	1.86	1.88	1.94	1.94	1.95	1.98	2.03	2.06	2.12	2.15
+400m	49.61634	1.15345	1.33045	46.81	48.93	49.4	50.3	53.2	46.81	48.51	48.73	49.11	49.2	49.4	49.65	49.91	50.54	51.1	53.2
+110m.hurdle	14.60585	0.47179	0.22258	13.97	14.21	14.48	14.98	15.67	13.97	14.05	14.17	14.25	14.37	14.48	14.78	14.95	15.01	15.29	15.67
+Discus	44.32561	3.37784	11.40984	37.92	41.9	44.41	46.07	51.65	37.92	40.22	40.95	42.1	43.34	44.41	44.83	45.62	47.6	48.95	51.65
+Pole.vault	4.76244	0.278	0.07728	4.2	4.5	4.8	4.92	5.4	4.2	4.4	4.5	4.6	4.7	4.8	4.82	4.92	5	5.02	5.4
+Javeline	58.31659	4.82682	23.29819	50.31	55.27	58.36	60.89	70.52	50.31	52.33	54.51	55.39	56.37	58.36	59.34	60.79	61.52	63.45	70.52
+1500m	279.02488	11.67325	136.2647	262.1	271.02	278.05	285.1	317	262.1	265.42	269.54	272.74	276.31	278.05	278.82	282	287.63	291.7	317