Mercurial > repos > wolma > mimodd_aln
changeset 0:d801b0675eb5 draft
planemo upload for repository https://github.com/wm75/mimodd_galaxy_wrappers commit b36048cd608ede0ec6f6559648525c9350caae34-dirty
author | wolma |
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date | Sat, 11 Nov 2017 18:18:54 -0500 |
parents | |
children | e76e813f615a |
files | macros.xml snap_caller.xml test-data/a.bam test-data/a.bcf test-data/a.fa test-data/a.sam test-data/a.vcf test-data/a_part1.bam test-data/a_part2.bam test-data/a_part2.bcf test-data/ce11ToCe10.over.chain test-data/header_only.bam test-data/header_only.sam test-data/reads_1_w_header.bam test-data/reads_1and2_w_header.bam test-data/rebased.vcf test-data/so_coordinate.bam test-data/so_queryname.sam test-data/split_pair_reads_1.fastqsanger test-data/split_pair_reads_2.fastqsanger test-data/vaf_linkage.pdf tool-data/all_fasta.loc.sample tool_data_table_conf.xml.sample |
diffstat | 23 files changed, 6023 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,46 @@ +<macros> + <token name="@MIMODD_VERSION_REQUIRED@">0.1.8</token> + <token name="@MIMODD_REAL_VERSION@">0.1.8</token> + <token name="@MIMODD_WRAPPER_VERSION@">0.1.8_0</token> + <token name="@HELP_FOOTER@"><![CDATA[ +---- + +.. class:: infomark + + For **additional help** see these resources: + +- The complete `MiModD User Guide <http://mimodd.readthedocs.io/en/v0.1.8/usage_toc.html>`__ + +- The `MiModD help forum <https://groups.google.com/forum/#!forum/mimodd>`__ reachable also via `email <mailto:mimodd@googlegroups.com>`__ + + ]]></token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@MIMODD_VERSION_REQUIRED@">MiModD</requirement> + <yield /> + </requirements> + </xml> + <xml name="stdio"> + <stdio> + <exit_code range="1:" /> + </stdio> + </xml> + <xml name="version_command"> + <version_command><![CDATA[mimodd version -q]]></version_command> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> +@misc{ +maier_mimodd_2014, +title = {{MiModD} - {Mutation} {Identification} in {Model} {Organism} {Genomes}}, +shorttitle = {{MiModD}}, +url = {https://sourceforge.net/projects/mimodd/}, +author = {Maier, Wolfgang and Moos, Katharina and Seifert, Mark and Baumeister, Ralf}, +year = {2014}, publisher={SourceForge.net} } + </citation> + </citations> + </xml> +</macros> + +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/snap_caller.xml Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,546 @@ +<tool id="mimodd_align" name="MiModD Read Alignment" version="@MIMODD_WRAPPER_VERSION@"> + <description>maps sequence reads to a reference genome using SNAP</description> + <macros> + <import>macros.xml</import> + <macro name="require_metadata"> + <param name="header" type="data" format="sam" + label="metadata source for this sample" /> + </macro> + <macro name="sam_bam_selector" token_format="sam"> + <param name="ifile" type="data" format="@FORMAT@" + label="input file"/> + <param name="header" type="data" format="sam" optional="true" + label="(optional) metadata source for this sample" + help="a SAM format dataset providing information about the sequences in the input data in its header; do NOT provide this dataset if the information is already part of your input dataset unless you want to have the original metadata overwritten. If needed, a metadata source dataset can be generated with the MiModD Run Annotation tool." /> + </macro> + </macros> + <expand macro="requirements" /> + <expand macro="stdio" /> + <expand macro="version_command" /> + <command><![CDATA[ + ## Currently Galaxy does not autoconvert collections of fastq.gz files. + ## This tool wrapper fixes that by allowing fastq and fastq.gz as input + ## collection formats. + ## gz_input is then used as flag to indicate a fastq.gz input file + #set gz_input = False + + mimodd snap-batch -s + #if str($reference.source) == "cached": + #set ref_genome = $reference.genome.fields.path + #else: + #set ref_genome = $reference.genome + #end if + #for $i in $datasets + "snap ${i.mode_choose.mode} '$ref_genome' + #if $str($i.mode_choose.mode) == "paired" and $str($i.mode_choose.input.iformat) == "fastq": + #if $str($i.mode_choose.input.pe_source.type) == 'collection': + ## PE input provided as a paired collection - if the forward + ## dataset is gzipped we assume the reverse dataset is too. + '${i.mode_choose.input.pe_source.input_data.forward}' + '${i.mode_choose.input.pe_source.input_data.reverse}' + #if $i.mode_choose.input.pe_source.input_data.forward.is_of_type('fastq.gz'): + #set gz_input = True + #end if + #else + ## PE input provided as separate fastq datasets + '${i.mode_choose.input.pe_source.ifile1}' + '${i.mode_choose.input.pe_source.ifile2}' + #end if + #else: + ## Input is either SE data or not in fastq format => + ## only one input dataset + '${i.mode_choose.input.ifile}' + #end if + #if $gz_input: + ## a gzipped fastq input dataset was encountered + --iformat gz + #else + --iformat ${i.mode_choose.input.iformat} + #end if + --ofile '$ofile' --oformat ${output_options.oformat} + ${output_options.sort} ${output_options.explicit_mmatch_notation} + --idx-seedsize $indexing.seedsize + --idx-slack $indexing.slack + --idx-overflow $indexing.overflow + #set $aln_spec = $i.mode_choose.aln_options + #if $str($i.mode_choose.mode) == "paired": + #set $aln_global = $alignment.paired + #set $treat_overlaps = $aln_spec.discard_overlapping_mates or $aln_global.discard_overlapping_mates + --spacing #if $aln_spec.sp_min then $aln_spec.sp_min else $aln_global.sp_min + #if $aln_spec.sp_max then $aln_spec.sp_max else $aln_global.sp_max + #else + #set $aln_global = $alignment.single + #set $treat_overlaps = "" + #end if + --maxseeds #if $aln_spec.maxseeds then $aln_spec.maxseeds else $aln_global.maxseeds + --maxhits #if $aln_spec.maxhits then $aln_spec.maxhits else $aln_global.maxhits + --clipping #if $aln_spec.clipping then $aln_spec.clipping else $aln_global.clipping + --maxdist #if $aln_spec.maxdist then $aln_spec.maxdist else $aln_global.maxdist + --confdiff #if $aln_spec.confdiff then $aln_spec.confdiff else $aln_global.confdiff + --confadapt #if $aln_spec.confadpt then $aln_spec.confadpt else $aln_global.confadpt + #if $i.mode_choose.input.header: + --header '${i.mode_choose.input.header}' + #end if + --selectivity $output_options.selectivity + #if $str($output_options.filter_output) != "off": + --filter-output $output_options.filter_output + #end if + #if $treat_overlaps: + --discard-overlapping-mates + ## remove ',' (and possibly adjacent whitespace) and replace with ' ' + '#echo ("' '".join($treat_overlaps.replace(" ", "").split(',')))#' + #end if + --verbose" + #end for + ]]></command> + + <inputs> + <conditional name="reference"> + <param name="source" type="select" + label="Will you select a reference genome from your history or use a built-in genome?"> + <option value="cached">Use a built-in genome</option> + <option value="history">Use a genome from my history</option> + </param> + <when value="cached"> + <param name="genome" type="select" + label="reference genome" + help="The fasta reference genome that SNAP should align reads against."> + <options from_data_table="all_fasta" /> + </param> + </when> + <when value="history"> + <param name="genome" type="data" format="fasta" + label="reference genome" + help="The fasta reference genome that SNAP should align reads against."/> + </when> + </conditional> + <section name="indexing" title="Parameters affecting reference genome indexing" expanded="false"> + <param name="seedsize" type="integer" value="20" + label="seed size (default: 20)" + help="Length of the seeds used in the reference genome hash table (SNAP index option -s)."/> + <param name="slack" type="float" value="0.3" + label="hash table slack size (default: 0.3)" + help="Corresponds to the -h option of SNAP index."/> + <param name="overflow" type="integer" min="1" max="1000" value="40" + label="index overflow factor (default: 40)" + help="Factor (between 1 and 1000) that controls the size of the index build overflow space. For certain genomes you may have to increase this value if you are getting a corresponding error from the tool." /> + </section> + <section name="alignment" title="Alignment parameters" expanded="false" + help="The global alignment parameters in this section will be used for samples for which you do not provide their own sample-specific settings."> + <section name="single" title="Parameters applied to single-end samples" + help="These parameters will affect the alignments for any single-end sample + for which you do not provide sample-specific settings."> + <param name="maxdist" type="integer" value="8" + label="edit distance (default: 8)" + help="maximum edit distance allowed per read or pair (SNAP option -d); higher values allow more divergent alignments to be found, but increase the rate of misalignments."/> + <param name="confdiff" type="integer" value="2" + label="confidence threshold (default: 2)" + help="Confidence threshold (SNAP option -c); the minimum edit distance difference between two alternate alignments required to reject the poorer alignment as suboptimal; higher values increase the rate of ambiguously aligned reads."/> + <param name="confadpt" type="integer" value="7" + label="adaptive confdiff behaviour (default: 7)" + help="Specifies how many seeds of a read may be ignored (based on the maximum hits value above) before the confidence threshold above gets increased by one for that read (SNAP option -a); helps fine-tuning alignment accuracy in repetitive regions of the genome."/> + <param name="maxseeds" type="integer" value="25" + label="maximum seeds per read (default: 25)" + help="Number of seeds to use per read (SNAP option -n) when trying to match it to the reference genome; higher numbers will increase the rate of aligned reads and reduce the rate of misalignments, but will reduce performance."/> + <param name="maxhits" type="integer" value="250" + label="maximum hits per seed (default: 250)" + help="Maximum hits to consider per seed (SNAP option -h); don't use a seed region in the alignment process if it matches more than maxhits regions in the reference genome. Higher values reduce the rate of misalignments, but reduce performance."/> + <param name="clipping" type="select" display="radio" + label="read clipping (default: from back and front)" + help="Specifies from which end of a read low-quality bases should be clipped (SNAP option -Cxx)"> + <option value="++">from back and front</option> + <option value="x+">from back only</option> + <option value="+x">from front only</option> + <option value="xx">no clipping</option> + </param> + </section> + <section name="paired" title="Parameters applied to paired-end samples" + help="These parameters will affect the alignments for any paired-end sample + for which you do not provide sample-specific settings."> + <param name="sp_min" type="integer" value="100" + label="minimum spacing to allow between paired ends (default: 100)" + help="Corresponds to the first value of the SNAP option -s."/> + <param name="sp_max" type="integer" value="10000" + label="maximum spacing to allow between paired ends (default: 10000)" + help="Corresponds to the second value of the SNAP option -s."/> + <param name="discard_overlapping_mates" type="text" optional="true" + label="discard overlapping read pairs of type" + help="Consider overlapping mate pairs of the given orientation type(s) anomalous and discard them; allowed values: RF, FR, FF, RR; multiple types may be specified as a comma-separated list and ALL can be used as a shortcut for discarding all overlapping mate pairs; leave blank to retain all overlapping pairs." /> + <param name="maxdist" type="integer" value="8" + label="edit distance (default: 8)" + help="maximum edit distance allowed per read or pair (SNAP option -d); higher values allow more divergent alignments to be found, but increase the rate of misalignments."/> + <param name="confdiff" type="integer" value="2" + label="confidence threshold (default: 2)" + help="Confidence threshold (SNAP option -c); the minimum edit distance difference between two alternate alignments required to reject the poorer alignment as suboptimal; higher values increase the rate of ambiguously aligned reads."/> + <param name="confadpt" type="integer" value="7" + label="adaptive confdiff behaviour (default: 7)" + help="Specifies how many seeds of a read may be ignored (based on the maximum hits value above) before the confidence threshold above gets increased by one for that read (SNAP option -a); helps fine-tuning alignment accuracy in repetitive regions of the genome."/> + <param name="maxseeds" type="integer" value="25" + label="maximum seeds per read (default: 25)" + help="Number of seeds to use per read (SNAP option -n) when trying to match it to the reference genome; higher numbers will increase the rate of aligned reads and reduce the rate of misalignments, but will reduce performance."/> + <param name="maxhits" type="integer" value="250" + label="maximum hits per seed (default: 250)" + help="Maximum hits to consider per seed (SNAP option -h); don't use a seed region in the alignment process if it matches more than maxhits regions in the reference genome. Higher values reduce the rate of misalignments, but reduce performance."/> + <param name="clipping" type="select" display="radio" + label="read clipping (default: from back and front)" + help="Specifies from which end of a read low-quality bases should be clipped (SNAP option -Cxx)"> + <option value="++">from back and front</option> + <option value="x+">from back only</option> + <option value="+x">from front only</option> + <option value="xx">no clipping</option> + </param> + </section> + </section> + <conditional name="output_options"> + <param name="config" type="select" + label="Output options" + help="No matter how many input datasets you specify below and what there formats are, this tool will produce a single output file with the aligned reads from all samples. In this section you can configure some aspects of what the output should look like. Unless you have a really special usecase, you can (and probably should) just go with the default settings though."> + <option value="default">Just go with the defaults</option> + <option value="custom">Show detailed output options</option> + </param> + <when value="default"> + <param name="oformat" type="hidden" value="bam" /> + <param name="sort" type="hidden" value=""/> + <param name="explicit_mmatch_notation" type="hidden" value=""/> + <param name="filter_output" type="hidden" value="off"/> + <param name="selectivity" type="hidden" value="1"/> + </when> + <when value="custom"> + <param name="oformat" type="select" display="radio" + label="Output format"> + <option value="bam">BAM</option> + <option value="sam">SAM</option> + </param> + <param name="sort" type="boolean" falsevalue="--no-sort" truevalue="" checked="true" + label="Sort aligned reads in the output by coordinates" + help="Turn off if you want to retain the read order of the input file(s) (mimodd snap option --no-sort)." /> + <param name="explicit_mmatch_notation" type="boolean" truevalue="-X" falsevalue="" checked="false" + label="Use = and X to indicate matches/mismatches in CIGAR strings explicitly instead of using M for both" + help="Warning: Downstream tools may still rely on the classic M notation! Turn this on at your own risk (mimodd snap option -X)." /> + <param name="selectivity" type="integer" min="1" value="1" + label="selectivity (default: 1)" + help="randomly choose 1/selectivity of the reads to score (SNAP option -S). The default of 1 indicates that all reads should be worked with." /> + <param name="filter_output" type="select" display="radio" + label="filter output (default: no filtering)" + help="filter output (SNAP option -F) to retain only specific classes of reads."> + <option value="off">no filtering</option> + <option value="a">aligned only</option> + <option value="s">single-aligned only</option> + <option value="u">unaligned only</option> + </param> + </when> + </conditional> + <repeat name="datasets" title="datasets" default="1" min="1"> + <conditional name="mode_choose"> + <param name="mode" type="select" label="choose mode" + help="Reads obtained from single-end sequencing runs should be aligned in 'single' mode, paired-end reads in 'paired' mode. **WARNING**: if the read input file is in SAM/BAM format, the current version of this tool will **not** verify the mode and may produce erroneous alignments with wrong settings!"> + <option value="single">single-end</option> + <option value="paired">paired-end</option> + </param> + <when value="single"> + <conditional name="input"> + <param name="iformat" type="select" label="input file format"> + <option value="bam">BAM</option> + <option value="sam">SAM</option> + <option value="fastq">fastq</option> + </param> + <when value="bam"> + <expand macro="sam_bam_selector" format="bam" /> + </when> + <when value="sam"> + <expand macro="sam_bam_selector" format="sam" /> + </when> + <when value="fastq"> + <param name="ifile" type="data" format="fastq" + label="input file"/> + <expand macro="require_metadata" /> + </when> + </conditional> + <section name="aln_options" title="Alignment options for this sample" expanded="false" + help="Any options you specify here will overwrite the global alignment settings defined for all single-end samples above."> + <param name="maxdist" type="integer" optional="true" value="" + label="edit distance" + help="maximum edit distance allowed per read or pair (SNAP option -d); higher values allow more divergent alignments to be found, but increase the rate of misalignments."/> + <param name="confdiff" type="integer" optional="true" value="" + label="confidence threshold" + help="Confidence threshold (SNAP option -c); the minimum edit distance difference between two alternate alignments required to reject the poorer alignment as suboptimal; higher values increase the rate of ambiguously aligned reads."/> + <param name="confadpt" type="integer" optional="true" value="" + label="adaptive confdiff behaviour" + help="Specifies how many seeds of a read may be ignored (based on the maximum hits value above) before the confidence threshold above gets increased by one for that read (SNAP option -a); helps fine-tuning alignment accuracy in repetitive regions of the genome."/> + <param name="maxseeds" type="integer" optional="true" value="" + label="maximum seeds per read" + help="Number of seeds to use per read (SNAP option -n) when trying to match it to the reference genome; higher numbers will increase the rate of aligned reads and reduce the rate of misalignments, but will reduce performance."/> + <param name="maxhits" type="integer" optional="true" value="" + label="maximum hits per seed" + help="Maximum hits to consider per seed (SNAP option -h); don't use a seed region in the alignment process if it matches more than maxhits regions in the reference genome. Higher values reduce the rate of misalignments, but reduce performance."/> + <param name="clipping" type="select" display="radio" + label="read clipping (default: from back and front)" + help="Specifies from which end of a read low-quality bases should be clipped (SNAP option -Cxx)"> + <option value="">use global setting</option> + <option value="++">from back and front</option> + <option value="x+">from back only</option> + <option value="+x">from front only</option> + <option value="xx">no clipping</option> + </param> + </section> + </when> + <when value="paired"> + <conditional name="input"> + <param name="iformat" type="select" label="input file format"> + <option value="bam">BAM</option> + <option value="sam">SAM</option> + <option value="fastq">fastq</option> + </param> + <when value="bam"> + <expand macro="sam_bam_selector" format="bam" /> + </when> + <when value="sam"> + <expand macro="sam_bam_selector" format="sam" /> + </when> + <when value="fastq"> + <conditional name="pe_source"> + <param name="type" type="select" + label="the paired-end fastq input is provided as"> + <option value="individual">Individual datasets</option> + <option value="collection">a Paired collection</option> + </param> + <when value="individual"> + <param name="ifile1" type="data" format="fastq" + label="inputfile with the first set of reads of paired-end data"/> + <param name="ifile2" type="data" format="fastq" + label="inputfile with the second set of reads of paired-end data"/> + </when> + <when value="collection"> + <param name="input_data" type="data_collection" + collection_type="paired" format="fastq, fastq.gz" + label="paired input dataset collection"/> + </when> + </conditional> + <expand macro="require_metadata" /> + </when> + </conditional> + <section name="aln_options" title="Alignment options for this sample" expanded="false" + help="Any options you specify here will overwrite the global alignment settings defined for all paired-end samples above."> + <param name="sp_min" type="integer" optional="true" value="0" + label="minimum spacing to allow between paired ends" + help="Corresponds to the first value of the SNAP option -s."/> + <param name="sp_max" type="integer" optional="true" value="0" + label="maximum spacing to allow between paired ends" + help="Corresponds to the second value of the SNAP option -s."/> + <param name="discard_overlapping_mates" type="text" optional="true" value="" + label="discard overlapping read pairs of type" + help="Consider overlapping mate pairs of the given orientation type(s) anomalous and discard them; allowed values: RF, FR, FF, RR; multiple types may be specified as a comma-separated list and ALL can be used as a shortcut for discarding all overlapping mate pairs; leave blank to retain all overlapping pairs." /> + <param name="maxdist" type="integer" optional="true" value="0" + label="edit distance" + help="maximum edit distance allowed per read or pair (SNAP option -d); higher values allow more divergent alignments to be found, but increase the rate of misalignments."/> + <param name="confdiff" type="integer" optional="true" value="" + label="confidence threshold" + help="Confidence threshold (SNAP option -c); the minimum edit distance difference between two alternate alignments required to reject the poorer alignment as suboptimal; higher values increase the rate of ambiguously aligned reads."/> + <param name="confadpt" type="integer" optional="true" value="" + label="adaptive confdiff behaviour" + help="Specifies how many seeds of a read may be ignored (based on the maximum hits value above) before the confidence threshold above gets increased by one for that read (SNAP option -a); helps fine-tuning alignment accuracy in repetitive regions of the genome."/> + <param name="maxseeds" type="integer" optional="true" value="" + label="maximum seeds per read" + help="Number of seeds to use per read (SNAP option -n) when trying to match it to the reference genome; higher numbers will increase the rate of aligned reads and reduce the rate of misalignments, but will reduce performance."/> + <param name="maxhits" type="integer" optional="true" value="" + label="maximum hits per seed" + help="Maximum hits to consider per seed (SNAP option -h); don't use a seed region in the alignment process if it matches more than maxhits regions in the reference genome. Higher values reduce the rate of misalignments, but reduce performance."/> + <param name="clipping" type="select" display="radio" + label="read clipping (default: from back and front)" + help="Specifies from which end of a read low-quality bases should be clipped (SNAP option -Cxx)"> + <option value="">use global setting</option> + <option value="++">from back and front</option> + <option value="x+">from back only</option> + <option value="+x">from front only</option> + <option value="xx">no clipping</option> + </param> + </section> + </when> + </conditional> + </repeat> + </inputs> + + <outputs> + <data name="ofile" format="bam" + label="Aligned reads from MiModd ${tool.name} on ${on_string}"> + <change_format> + <when input="output_options.oformat" value="sam" format="sam"/> + </change_format> + <actions> + <conditional name="reference.source"> + <when value="cached"> + <action type="metadata" name="dbkey"> + <option type="from_data_table" name="all_fasta" column="1" offset="0"> + <filter type="param_value" ref="reference.genome" column="0" /> + </option> + </action> + </when> + </conditional> + </actions> + </data> + </outputs> + + <tests> + <test> + <conditional name="reference"> + <param name="source" value="history" /> + <param name="genome" value="a.fa" /> + </conditional> + <repeat name="datasets"> + <conditional name="mode_choose"> + <param name="mode" value="single" /> + <conditional name="input"> + <param name="iformat" value="bam" /> + <param name="ifile" value="a_part1.bam" /> + </conditional> + </conditional> + </repeat> + <assert_command> + <has_text text="--idx-slack 0.3" /> + <has_text text="--iformat bam" /> + <has_text text="--oformat bam" /> + <has_text text="--idx-seedsize 20" /> + <has_text text="--idx-slack 0.3" /> + <has_text text="--idx-overflow 40" /> + <has_text text="--maxseeds 25" /> + <has_text text="--maxhits 250" /> + <has_text text="--clipping ++" /> + <has_text text="--maxdist 8" /> + <has_text text="--confdiff 2" /> + <has_text text="--confadapt 7" /> + <has_text text="--selectivity 1" /> + </assert_command> + </test> + <test> + <conditional name="reference"> + <param name="source" value="history" /> + <param name="genome" value="a.fa" /> + </conditional> + <repeat name="datasets"> + <conditional name="mode_choose"> + <param name="mode" value="single" /> + <conditional name="input"> + <param name="iformat" value="bam" /> + <param name="ifile" value="a_part1.bam" /> + </conditional> + <section name="aln_options"> + <param name="maxdist" value="7" /> + </section> + </conditional> + </repeat> + <assert_command> + <has_text text="--idx-slack 0.3" /> + <has_text text="--iformat bam" /> + <has_text text="--oformat bam" /> + <has_text text="--idx-seedsize 20" /> + <has_text text="--idx-slack 0.3" /> + <has_text text="--idx-overflow 40" /> + <has_text text="--maxseeds 25" /> + <has_text text="--maxhits 250" /> + <has_text text="--clipping ++" /> + <has_text text="--maxdist 7" /> + <has_text text="--confdiff 2" /> + <has_text text="--confadapt 7" /> + <has_text text="--selectivity 1" /> + </assert_command> + </test> + </tests> + + <help><![CDATA[ +.. class:: infomark + + **What it does** + +The tool aligns the sequenced reads in an arbitrary number of input datasets +against a common reference genome and stores the results in a single, possibly +multi-sample output dataset. + +Internally, the tool uses the ultrafast, hashtable-based aligner SNAP (http://snap.cs.berkeley.edu). + +---------- + +**Notes:** + +*Input formats* + +- The tool accepts SAM, BAM, fastq and fastq.gz input datasets of sequenced + reads and supports both single-end and paired-end data. + + The recommended approach with MiModD is to store NGS datasets in SAM/BAM + format with *Run Metadata* (see below) stored in the file header. You can use + the *MiModD Run Annotation* and *MiModD Convert* tools to convert data from + fastq format to SAM/BAM format while attaching run metadata to it. + + While alignments **directly from fastq format** are supported, this **is less + reliable** due to less strict specifications of this format. If you find + the tool complaining about malformed fastq input, it is likely that you can + fix this problem by converting the data to SAM/BAM format first. + +- If you wish to align paired-end data directly from fastq format, the mate + sequence data has to be split over two datasets as is mostly standard today. + If you have your paired-end data as a single dataset you may look into the + *FASTQ splitter* and *FASTQ de-interlacer* tools for Galaxy, which are + available from the `Fastq Manipulation category`_ of the Galaxy Tool Shed and + may be able to convert your files to the expected format. + +*Run Metadata* + +- **Every input file requires accompanying Run Metadata!** Most importantly, + this includes a *read-group ID* (an identifier of the sequencing run that + produced the data) and a *sample name* (identifying the + biological sample sequenced in the run). + +- If an input dataset does not provide this information directly (fastq + datasets never do; SAM/BAM datasets may provide it in their header), you need + to specify a separate SAM/BAM dataset with an appropriate header as the + source of the Run Metadata. + + You can use the *MiModD Run Annotation* tool to generate such a file. + +- If a SAM/BAM input dataset already provides Run Metadata, you can still + specify a different Run Metadata source, which will then overwrite the + information already present in the input. This is useful, for example, to + resolve read-group ID conflicts between multiple input datasets. + +- Every input dataset can only contain reads from a single read-group. If you + would like, for example, to realign the reads in a multi-sample SAM/BAM + dataset. You should first use the *MiModD Sort* tool to sort the data by read + names (this step is only necessary for paired-end data), then split the reads + into new per-read-group datasets using the *MiModD Convert* tool. + +- Several input datasets can declare identical read-group IDs and/or sample + names. + + Identical read-group IDs mean that the datasets were produced in the + same sequencing run, as is the case, for example, with partial fastq + sequencing data. In the output dataset, the corresponding reads will be + merged and it will not be possible to trace back their source. + + Identical sample names (but different read-group IDs) indicate that the same + sample has been sequenced multiple times. In the output dataset, the + corresponding reads will be tagged appropriately and tools like the + *MiModD Variant Calling* tool will let you decide whether you want to treat + them together or separately. + +---------- + +**Tool Options** + +The section *Alignment parameters* lets you configure global settings for the +alignment job that will be applied to all input datasets. For each input +dataset, however, you can overwrite some or all of these settings by specifying +new values in the section *Alignment options for this sample*. Some of the +alignment parameters may have **big** effects on the alignment quality, but +these effects are very dependent on the type of input sequences. You are +strongly encouraged to consult the in-depth `tool documentation`_ for detailed +explanations of the available options. + +.. _Fastq Manipulation category: https://toolshed.g2.bx.psu.edu/repository/browse_repositories_in_category?id=310ff67d4caf6531 +.. _recipe for using gzipped fastq files in Galaxy: http://mimodd.readthedocs.org/en/latest/recipes.html#use-gzipped-fastq-files-in-galaxy +.. _tool documentation: http://mimodd.readthedocs.io/en/@MIMODD_REAL_VERSION@/tool_doc.html#snap + +@HELP_FOOTER@ + ]]></help> + <expand macro="citations" /> +</tool> +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.fa Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,3289 @@ +>chrI +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaa +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaa +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaaaaaattgagataagaaaa +cattttactttttcaaaattgttttcatgctaaattcaaaacgttttttt +tttagtgaagcttctagatatttggcgggtacctctaattttgcctgcct +gccaacctatatgctcctgtgtttaggcctaatactaagcctaagcctaa +gcctaatactaagcctaagcctaagactaagcctaatactaagcctaagc +ctaagactaagcctaagactaagcctaagactaagcctaatactaagcct +aagcctaagactaagcctaagcctaatactaagcctaagcctaagactaa +gcctaatactaagcctaagcctaagactaagcctaagactaagcctaaga 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.sam Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,824 @@ +@HD VN:1.5 SO:coordinate +@RG ID:000 SM:N2 +@RG ID:266-1 SM:ot266 DS:mutant strain carrying the vab-3 allele ot266 CN:Columbia University PL:Illumina +@SQ SN:chrI LN:25050 M5:7d9f340160ce45568f9bf48d8e16dcf4 +@SQ SN:chrII LN:25050 M5:ba5a89bf12f55a4bd88ddf14e6d27bd6 +@SQ SN:chrIII LN:25050 M5:ba78739f84b6c850448bec286bdd8798 +@SQ SN:chrIV LN:25050 M5:0807d4df5c3593363a5b015f19ef81c2 +@SQ SN:chrV LN:25050 M5:cf942c1556ef72fd9ba352e4a7d9cf9e +@SQ SN:chrX LN:25050 M5:5ce1b12d74140cfc288b493c16fe280b +@SQ SN:MtDNA LN:13794 M5:199e147d502d88e45047413dc83c039c +@PG ID:SNAP PN:MiModD snap VN:0.1.8.0 CL:paired /var/tmp/batch_dataset_548966_dataset_35_20_0.3 /var/tmp/batch_dataset_54896612531.sam -d 8 -S 200 -n 25 -a 7 -F s -o /var/tmp/batch_dataset_54896615435.tmp -h 250 -c 2 -C++ -M -s 100 10000 -t 2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217 99 chrI 556 60 100M = 702 246 CCTATATGCTCCTGTGTTTAGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAG CCCFFFFDHHHHHJJJJJJJIJJJJJJIIIIIJIJJJJJGIIJIIHIJIJJHIJIJJHIJJIIJJJIIIIGGGIJHHHHHFFEFFFEECEEDDCACDDC? PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:56:17087:17617/1 0 chrI 575 60 101M * 0 0 AGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCC GGFGGGDGFGEFBGFGFGGGGGGFGGEGGGGGGGGGGGGGGGGGEGGFFEFGGFFGEAFGFDEGGGGGGGEEFDDEEEEEGFGGEGEDBEEAEDEECFEBC PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217 147 chrI 702 60 100M = 556 -246 AGCCTAAGACTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCCTAAGAC CCC>AA>;(CCC;C@3HEEEGCIGED=EGGGJHGJIGGCIJIIDFGGIGDIHJIHEGIHJIIEGGIIGJJJIGIGJIJJJIGDIHGIHHFHGFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:81:6795:13435/1 16 chrI 842 60 101M * 0 0 TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA A:@EEEEEBEEEEEEEEEGEEEEGEEEEEEEED=BFEEEGEEGEGFGGGEEGGGGGGGGFGGGFDDAGG=GGEGGGFFFEFEECBFDGGEGDGDGGGFGEG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:46:9721:15037/1 16 chrI 842 60 101M * 0 0 TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA AEEBEBBEEEAAEFEFFFE?FCCCBC?DDBD5DD=D:DDC:CC?C>6EB@CEEEDDEECEEEEE8BFFEEFEEADDCCC@CBCCEEFDFFAEFGFDFGGGD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:77:15674:6412/1 0 chrI 844 60 101M * 0 0 GAAATGAATATCGTAGCTACAGAAACGGCAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAACATA GFFGGGCGDFGEGDFEEFEGGFEGFEEEACC?BCCDDCDECBECDEFEECEEDEEEEEEEEBEEEEEEAEEEEE=AC:=CBBCCCC@::?CACC@BBBABE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:88:10221:3318/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAG AEBABBEEBGEGEBGGGEEEEGEEGGEEGGCEEEFEFEEBGGFDEGAEFGGEDFF=EEGEGGGGGG?DBDFGGFGGDFECCFDDCGEEEE:AG?GFFGDGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:87:4119:14575/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA GEEGEDEEGEGGEAEFEB?DEEDBGGBGGFGEFBGFGGBGGGGGFGGEGFGGGGGGGGGGGGFEFAGGGGEGGDGGGGDFGGBFGGGGDFDGGDGGGGGEG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:95:9634:15406/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAA FEEE?GEFEBE:ECE=BEGGGEGEGBFFFEFAFDFFGEBBGGGFGDGEGGGEEEEE5FFFFCFGAEGGEGGGGGGGFGDGGGFGGGGGGGFGDFFFGGGFD PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793 73 chrI 846 60 100M = 846 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA CCCFFFFFHHHHHJJJJIIIIJIJJJFGIGIIJJIGIJIJJGEHIHJHEHHHJIIJJJJJIJJIGIJIHHHHHHEFFFFECEEDBDDCCDDDDD?CDDDC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793 133 chrI 846 0 * = 846 0 GTGTACTACCGTTTCTGGAGCTACGATTTATTTTTCAAAACACGCAAAAAGTGTTTTTCACTATGTACAAAATCATCTCAAAATCGGAAATATTTTTTGT <<<@@@@@@@@@@???#################################################################################### PG:Z:SNAP RG:Z:000 +EAGLE:2:45:11395:10768/1 0 chrI 846 60 40M3I58M * 0 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCTAAATAT G?FEGGBGGEFDFDDEDEBEEDFFEC@D=AFFFCFFFFFEAFEDFFEF=FFFFFFFD>DF?FFAFFFFFECCAFE=BEAEEFBDEEFEEEEEEEEBEFDDA PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:47:11205:5807/1 16 chrI 846 60 101M * 0 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAG FEEDBBFEAEFDEFEE?EDFEEACFEDAFDDFEFFFFFFFFFFFFEEBFFEFEFFFFFFFEEFFFFEEFFFFFFFEEACAFDGGFGGGGGGGGGGGGDGEG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:69:6499:16127/1 0 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC GGGGGGGGGEGGGGGGGGGGGFGGGDGGEGGGGGGGGGGGFGGGGGGDFGGDGEGGFGG=EFFFFAG;CGGEBGEEFEFGGGGF?CEBCFFEBFEEEEF?F PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:24:6173:14843/1 0 chrI 847 60 101M * 0 0 ATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC FDGFGGDEGEEG?GGAEEEEBEE?::AB;AADB=CEFFFD=EDFF?EBEEEC@@EFDEEDADEDFE;EAFD::D:BCB<BEEDB8=DB??BBB5:=@:@@: PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:52:5204:4050/1 0 chrI 847 60 101M * 0 0 ATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC FFFFEFFFFAEAEAEGGGGDEGGGGBFEDEFFFFFGGGGF=FDFFGGGGGGGAEGGGGGFFFGFGGGAGEEGFG?GFDEEGFFGBEEEEEFEDGFDG5FFB PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:88:14674:10686/1 16 chrI 847 60 101M * 0 0 ATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC EEEEAEBBEDAEEEEEFDFDDEGEGGEDFBEBFEFEBGCEGCEE?8DEFDEFGGGEEEEC>?FEGGFEGGGCEEBE:CCEEEDFGG=EFBGGEBFGGGDDG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:29:17996:20445/1 16 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EE5?EEBBBB?E=DEDFEFEBADCEECBAAE=E=EBDF?EGEE=GCDFFEDBDFGGDGGGGEF=GGGGGBDGGGDGBGGFGGGGGGGFEGGGEEGEEGGFG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:112:10138:4083/1 16 chrI 847 60 101M * 0 0 ATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EC?@BEACB:C@=EE?CAECC@CCCE>=A<55CCD?BEE?E?FFA;GGGGGEGEGEEDBGGGGEGBGGEGGBFGDGCCCGEG?GDGDFFGGFFFFFE?EDE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:54:15434:16430/1 16 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EEEE@EEEEEEDGEEEGEGDEEGGGGEG?DFEGFGGFFGGGFGGEEFGGGGGGGGGGGGGGGGEGGGGGGGFGGFGBGDGGGEGGGEGGGEGGGFDDGGGF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:58:7649:14576/1 0 chrI 848 60 101M * 0 0 TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTATAGCTAAGACACTTTTTGTCTGCCCAAATATAAGCA GGGGGGGGGGGGGGGGGGFGGGGGEFFEDFEGGGGGGGGFGGGGGGCFFGGGGEFEGGFGGGGGFGGGGGFFFGGGGEGGGGEEEGGGGGFEGEF?FBBFG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:76:11197:2138/1 0 chrI 848 60 101M * 0 0 TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA EEDFFFEFBDADEEEFFBFFFEFFE=C@?CFFFFFFFDF?EEEDEFBDFFFFFFFFFFAEAEDEBC;FFEFEFFDDABFFFFE;CFEFEEBEFBDBDDDEB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:59:14370:7837/1 0 chrI 849 60 101M * 0 0 GAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAAACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAA FGFGEGGEBGFGFGGDGFFGEED=EA@=DDFFE?FEFFFFFFFFFEEEEEFEEADFAFFFFFFFEEFEEBEEBEFEDEEEFEEEEBEEBE:EBBBB@BBAD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:79:15239:5534/1 16 chrI 849 60 101M * 0 0 GAATATCGTAGCTACAGAAACGATAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCACAAATATAGGCAA BFDGDDFFEDEGGDGFEEFBEFFFFEECECEGAEEGGGGFGGGEEGGGEGGBFGGGGGFGGFGGGFGGDEGGGGEGGFGGGGGEFGEGFGDFGFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:83:6103:1248/1 16 chrI 849 60 101M * 0 0 GAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAA @>B:9?BBBBDBB?DBEBEEEBDDDDD?FAEEEEEEEEEEEEEEFFEFFFEDFEFEECCDAFFFFFFFFFFEBE?@EDFFEFFEFFFFFFFFF?DFEFFDD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:60:3721:11022/1 0 chrI 851 60 35M3I63M * 0 0 ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA DGBGGGFGFFE=EEEDEFFFEBEEEED=EDFDFDFFFFE:FEFEDFDFFFEFFFFFFEDFFFFEFCAFFBBEEBEFFEEEFEAEEEEEEFEFFEEEEBEEF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:56:9356:7127/1 0 chrI 851 60 101M * 0 0 GTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACC GFGGGGGGDGGFGCFDBFFFGDGGDGEFFGGDFEEFGDGGEGGGFGGEEEG?GFGEEEFEED;@EGEBEEEEE?BDDDECEEEEEGEE?EA?AC@CCA5?? PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:26:13486:6669/1 16 chrI 851 60 101M * 0 0 ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACC GE:EEEDDDDAEGEBEEEFEGEEDABFCDEEEEEGGGEGGEEGGFGEDEEFAGDGEGBGFFGGGEGEGGGDFACCFFFGGDGGGEEBGGGEEEEEEEE?EE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:90:7286:17726/1 0 chrI 852 60 101M * 0 0 TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA GGGGFGGGGGGGGGFEGFFGFDDBGGFGGGEFGGGDAGEGGGFDGGDGGGGGGGE?GGGGGGEGGGGDFGGGGEEFBCEEDGFFBGGDGDGGEDBEEGDEB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:60:11520:19697/1 0 chrI 852 60 101M * 0 0 TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA DEDEEFFFFFEGGGFFFFFFBEE?EEEEEEFBFFFFFFF?FFFFFCFFCFFDFFAEFFFFEEADFDDF?EFFFEFFFBEEEEFEFFEFF@FDED:BEEBEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794 73 chrI 853 60 100M = 853 0 ATCGTAGCTACAGAAACGGTAATACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCCCAAATATAGGCAACCA @@@FDFFFHHFHHJJJIJJIJJJJJJJIIJGGIIGGIIII4?GIJJIIJIJJJJGIJJGGIIJJJJJDEHGEFCDCEDEEEEDCDDBDDDFEDCDDDDDB PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794 133 chrI 853 0 * = 853 0 GCTTCACAGTATATTTTACTTTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTAC A>;6;6(CA;;@B?3DB=AA7EGGIJIIGGIGEIJJJJJHEGGCBFGGFBBFG@IGDJIIJJIIJJIJJIGHIGHGJGHGIHCJJJIHHHHHFFFFFCCB PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478 73 chrI 853 60 33M3I64M = 853 0 ATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAA @CCFFFFFHHHHHIIIIJJHDHCHIIHIIJIJGIEGGHEDFFGEGIIJIJJJJGIJGGGIJIGEGGGJIHDHEH;?CDFFDECECAA?>BBDDDE>:CCC PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478 133 chrI 853 0 * = 853 0 GGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTC ?;;5::>CDED@DEFDDDDFFFFFFHHHHHJIIIEJJIJJJJJJJJIIHFIGGIIGJJIGJIJJJJJIJJJIJJIJJJJIJJIIIIIHFFAHFFFFFCCB PG:Z:SNAP RG:Z:000 +EAGLE:2:96:9394:5489/1 16 chrI 854 60 101M * 0 0 TCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAA EGEBEEEEEFEGEBEGEEE:E?DFCFFFGEGGFGGGFGEFGGGFGEEEGGGGEGGGGFGGGGGDGGGGEEGGGGGFGGGGGGFGGGGGGDGGGGG?GGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:110:3453:13829/1 16 chrI 855 60 101M * 0 0 CGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAA EAEAE?5EEEEC5=EBDFFDFDGEGEGGDBEFGGBFAEEGGGGGGEEEEDEE8FFFGGGGFGEGGGFFEEEDG?GGGDGGGGGGGFGAGDEGGGAFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:34:16346:19478/1 16 chrI 856 60 101M * 0 0 GTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAA DDD?BB@B=BBB>DDC@@B?:A?:AAEEDEABCCB6EEEEEBEEDDED:DC>EEEEBDDCBD5CEBED=CEEBEEEEEEDEAEEAEEFGGFBEBEAEEDEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:75:1484:17855/1 0 chrI 857 60 101M * 0 0 TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAGTTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAAT DD@CADEEEEEEFFDBDDCDEEDEEAFDEFAFFFFDFFFFFFD=FDFFFFFFFEF;CCEEDDDFDBEAAFFFFF8EBDEDDDFFAFDFFBFFFEF5FFEF= PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:110:6822:2912/1 16 chrI 857 60 43M1I44M1I2M1D10M * 0 0 TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTGATAGCTAGGACACTTTTTGTCTGCACAAATATGAGCAACCAAAAA DBFBDCECFEDEFGFGEGFEGEGGGGEFFGGEGF=BGGGGGGGGGFGGGGGGFGFGGGGGGGGGGEGCGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933 83 chrI 858 60 100M = 876 118 AGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAAT CCCEEDDDDBDBCDEDDEDCDDDDDDDCEEEEEEEDFHHHHHJIJIJJIHJJJJJJJJJJJJIGGBJJJIJJJJJJIIJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:2:91:13868:11992/1 0 chrI 859 60 41M1I59M * 0 0 GCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTCATAGCTAGGACACTTTTTGTCTGCACAAATATAGGCAACCAAAAATA GGGEGGGBEDGGECEEDFFFGGGGGGGGFGGGEGGGGFFGGGGAG=FFBDFFGGGEAFGBGEGBGGAEGEGFEEEEDEEEDFF=ECBCCC?AECCCDDECE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:119:12921:6612/1 16 chrI 859 60 27M3I71M * 0 0 GCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAA BBEEGEE=FBEEFDFGFAGBGGGFEGEFGDGEEGGAEGGGDGGGGDDFGDGGEEGEGGGEGEEEEBEBCCEFDGFFGDGGEFFGGFEEEEEFFBFFGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:111:6913:8666/1 0 chrI 860 60 101M * 0 0 CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAAT GGGGGGFFFGFF@EEAEEEEBGGGFGGEGGGGEGGGDGEDCFA5F?EEEEGGG>GGBFEEFFEGGEFFAFF>EDEC?FEDABBBBD=A:D=B=EB@E@?;> PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698 83 chrI 860 60 100M = 871 111 CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAA CEDDDC?BBBCDEEEECC@EDEEECEFFFFEFHHHJJJJJIJIJIJJJJJJJJJIJJJJJIGGGJJJJJJJJJJJJJIIJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:3 +EAGLE:1:67:7435:14824/1 16 chrI 861 60 101M * 0 0 TACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATT ::GEEGEEGDGGGEGEGDDBGG?GGGGFGGG@FFAFEGGFFEFGDGEFGGGFFGGGFFFFFGBFGGFGDGGGGGGGGGEFGGBGGGGFFGGGFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:11:15002:3991/1 16 chrI 862 60 101M * 0 0 ACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTT DEEFEDBEFEFDFBDF?EEFFDG?GEFEFC=EGAFGEGDFDFGGEEG=?GGGDDDFCEEBE6@@>A=GFFGGCFGGEEEEE:E?EEAEDEECEEE?5EEEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:96:4105:9079/1 0 chrI 863 60 101M * 0 0 CAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC GGGGDGGGGEGGEGGDGGGGGGGDGGGGGFGGGEGGFEBGDGGEGEG?GEFEEGEEEEEGDDEDEFEBEECC=DFDDDCCEAEBGEECGE@DEF???;?BE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778 73 chrI 864 60 100M = 864 0 AGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC BCCFFFFFHHGHHJJJJJJJJJJJJJHHGHHJJJJJJJJJJIIJJJJJJJIIJJJIJJJJGIJJJJJHHHHHHFFFFFEEEEDEDDDDDDDBBDDDEDEC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778 133 chrI 864 0 * = 864 0 AGAGTGTACTACCGTTTCTGTAGCTATGATACTCATTTCAAAAAACCCGAAAAATGATTTTTTCAATATGCAACAAATCATCAAAAACTTGGAAATTATT BCCFDFDFHHHHHJHJIIJJJJJJJJJJJFJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJIJHFFFFFFECDCEEBDDDDDDDDDDDDDDDDDDDDDDED PG:Z:SNAP RG:Z:000 +EAGLE:2:89:4630:12148/1 0 chrI 865 60 101M * 0 0 GAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCA GFEGGFFFFFB=DDCGGGGGEEFFFDFFFFGGDGGDGG=EDCGGGGGEG>GGFGFBFFFBGEGGGDCC=FEEEEEEE=E:EBEEEFGF?GGCAA8;EEBE: PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:71:6779:3992/1 0 chrI 866 60 20M3I78M * 0 0 AAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTC GGGGEFGGFFEGGFEGGGGGBGFEGFGGFEGGFGEACCFFDGEBFGGGGFGEGGADBFEFGGEE5EEE=ABBFEGGGGBFEE=EEEDGGBEEE=E@B;CAE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:90:8794:13563/1 0 chrI 867 60 101M * 0 0 AACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAG FFFFFEDFFFGFFFGGGGFFEGGGFFGGGGFGDGEFFGGAGGDGFGGGGFFCGGDGGGBGDF:F;ACEEEFF:AEDEEAEEDECGEGFGGE8C;DD=BD?B PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797 73 chrI 869 60 100M = 869 0 CAGTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGT BCCDFFFFHHGHFJJGGJJJJJHJIJIJJJIIJIJGIJJJJJJJJJIIIJJJIGHHGGGIGEHGIGEHEGHFGGHCCFBDFFEDDDDDDDDEFEDEDCCD PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797 133 chrI 869 0 * = 869 0 GTGTCCTAGCTATAAAAATTGCAAATTTTTTGTATTTTCAGAAGAGTGTACTACCGTTTCTGTAGCTACGATATTCATTTCAAAAAACCCGAAAAATGTT CB@DFFFFHHGHHJJJJIGGIJJIJIGIIJJJHIIIJIIJJHHIIIGHHIIJJJJJJJJJJJIJJJJJJJIHHGHFFFFFFFEEEDDDDDDDDDDDDDBD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698 163 chrI 871 60 100M = 860 -111 GTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT CBCFDFFFHHHHHJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJIJJIDHGHIIJJJJJJJIJJJEIIEEHHHHGFFFFDDDDDDDDDE<@DC5@@>> PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084 73 chrI 871 60 100M = 871 0 GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT @@CFDDFFHHHHHJJJJJJJIJIJJIIJIJJJJJJJJJJJJJJJJJJJJJIIJGIJJJJHHFHHHFFFFEEEFEDCCCDBDDDDDDDDDEEEDEECDDDC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084 133 chrI 871 0 * = 871 0 TTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTT BBDDDDDDDBBCECEEEEFFFFFFHHHJJJJJJJJJJJJIIIJJJIIIGJJJJJGJJJJJJIHFJJJIGJJJJJJJJJJJIJJJJJJHHHFHFDDFFCCB PG:Z:SNAP RG:Z:000 +EAGLE:2:85:11800:16523/1 16 chrI 871 60 101M * 0 0 GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTT GEGGEEFEGEGEEGEGGFGGE@GGGGGGGEGGGFGEGEGEGGGGGGGFGGEGEGEGGFGGGGFGGGAGGGGGGGGGGGGGGGGGGGGGGFGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:50:15754:14085/1 0 chrI 872 60 101M * 0 0 TAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTT FFFFFFFFDFFAFFFFBDFFFFFFFFFFFFFFFFBFFFFFFFEFFFFFFFFFFEFFFFFEEFFFFFFFFFEFFBFEFFEFFF?FFDEEEEFFEEEF@DFEA PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:51:4256:7814/1 0 chrI 873 60 101M * 0 0 AGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATTATTTCCAAGTTTTTG EFFDGGGGGEGGGFAFFGGGGGGFFGGGGFGGF?DGFGGGGGFGGFFAFGDGEDEGGGEGEFGGGEGGGDDFGGFGGEGBGGEFBGFEEEEEEBEEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:83:2757:15592/1 0 chrI 874 60 101M * 0 0 GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGA FEFFFFDFFEGBGGGGFDGGGEFFEDGGDGFBEAFFFFEACDGDEEDGGDEGEGGGGEEDEEEDDAE?EDBEEEBEEEBBGEEE@@@@BDD@?B@@BBB@; PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:74:11870:7997/1 0 chrI 874 60 101M * 0 0 GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGA GGEGGGGGGGGGDFGGGGFGGGGGGFDFFGGGGDFGGGFGGGGFGGGFGGGFEGGDB@GEGEGGGBGEFGEEGEEGEGEEBFEDCEEEE?EC@FACFDFDC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933 163 chrI 876 60 100M = 858 -118 ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGAT CCCFFFFFHHHHHJIHJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHDFFFDDEEEEEEDEEDDDDEDDDBC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:89:11320:10564/1 16 chrI 876 60 101M * 0 0 ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATG @EEFEGFEGFEG=GDFEGGEGGEGEGFBGGGEGFGFGGGGGGGGFDFEGGGGGDGAFGGFFGFGGGGFGGGDGGGBFGGGGGFGGGGGGGGGGGDGDGGFG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:87:16027:10849/1 16 chrI 880 60 101M * 0 0 TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAATTTTTGATGATTT ?BBBB@BBBB?;<BBB?BBBBBBDAB88?:?=BBBBBAADA?B;<<BC?BBBDDDABBADCA=B=?@@=5>@A:C?BC??DBEABDE=EEDDDD??DDDBD PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:98:7014:9919/1 16 chrI 880 60 101M * 0 0 TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT =BGECGFEEGG>?EFEAFEFAEGGABG@DBEFDGDGGFEEGEDCCGGFGDGDEEEDEFGFGDGGGDGGGFGGEGGGGFGGFDGGFGGGGGGEDGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:53:7732:11243/1 16 chrI 882 60 101M * 0 0 TTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGT E@D=DF@BB:8EFDEEBB==B?FEEEAABBAEFFFDECEEEBB@EDCE?CAE:EAE?AE=EEEE?ED=D<>EDAF?DDFBFAE?EE??BFAEEDEEFEDF? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:65:9151:20950/1 0 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GFGGGFGGFGGGEGEGGGGGGFEGGGEGGDGGFGG?GGGGGGGGGFGEGGGGGGGGGFGFGFGGFGFGGEFGGGEE@EDFEBBE?EEEEEBBEBDEEEEE? PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:96:16607:9367/1 16 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GEEGEGFCCFAGD:GEEAGFBGGGFGE=DDE:BFFFDCE5@AAFBDAEFBCCGFFFAFFFEFDDFDDBB@ABF?FDEGAGEFFFFAGGGDGEFAF?DEFFF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:99:9928:13510/1 16 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GEFEEGEEEGG?GGGGEBEGEFEEEBEGFGGEGFGFFCFCEEFFFFGFGBGFGEGFGGGGFGGEGGGGDGGFEGGGGGBGGGGGGGGGGGGEGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:88:16505:15331/1 16 chrI 885 60 101M * 0 0 TGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGC BEFGEDEEGGEEDEEDDEEEDEEGEEGBGGEEEEEEDF?CCGGGGGGFGGFFGDDGGGGGFGGGGGGFFFGGEFDGDEFFDGFFGGGGGGGGGFFGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:78:3200:13969/1 16 chrI 886 60 101M * 0 0 GAAAATACAAAAAGTTCGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA @B:BA88EEEE?=BBB=CEB8>DE?EEFCBFCBCCBE@B@C;DEFFFEFFFBFFDFFFFFFFFFFEEEBFFDEE5EBEEEEEAEE?EEE?DD;DEEFEF=D PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:87:17306:17581/1 16 chrI 886 60 101M * 0 0 GAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA AABE=8;AAA<?AA?:=EDBC@DFFFACCC=BADEEDB@;AFEFFFFDFDFEDAEEBEDEEEDEEEBCEEADFDFFEEE=AFFFFFEEFBBBEFFDEAAD? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:48:14507:16490/1 0 chrI 887 60 101M * 0 0 AAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCAT FFFFAGGGDGGGAGGFDFFFADEDEDAEDDFFB:FEAEEAEABBA::@C@BE?EECE?BB?CDACBAABEE?C=ABBB@B>==A=AAAAAEEEEEBD?BB: PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:54:4351:16632/1 16 chrI 887 60 101M * 0 0 AAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCAT EGEGAEGGGGGEGGEGGGGEGGGGGGGGFFGGGGGGEGGFFGGGDGFGFGGGFGFGGGGGGGGGGGDDFFF:EGGEGGGEGFFGFGGGGGGGGGGGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:115:17415:17791/1 0 chrI 888 60 101M * 0 0 AAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA DD@D7EEEEE>CCEFFFFDFFFFFF8EFFFEFFFFFFBFFBFF:DDFFFFFFDFDFF?E@DEBAEFDFF;C@C?EE=DADEEEE;D:DD@EEE?BE>E=ED PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:9:16465:5809/1 16 chrI 888 60 101M * 0 0 AAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA D?FDGDGGGGFDGGGBB=DGEFEGGGFFDFFEEEECD=GEGGFGGDGGGGG?FGFGGFGGG?GGGGGGGEGGEGFGGGGGFGGDBGFEGGGFFF:FGGGBG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:98:3753:20118/1 16 chrI 889 60 101M * 0 0 AATACAAAGAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATAT BGBDFGFFFBF=BDFGGEFFGGG?GE?FDEFECEBB>FF=FEDGFDF=EFBGFGGGEEDADE>AAEFDGAGDBGFGDFFFE?FDEEGEEDDFEDFFDADG= PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:33:4809:11289/1 16 chrI 890 60 101M * 0 0 ATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATT =EEFEGFFF=BDBBGG=EGGBEDGGG:DBEF?GCFGGDGFGEGEFGGEGGEGGDGGGDFDGCGGGGFFGGGGGGGGGGDFGFFGFGGFGGGGDGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:71:4682:12647/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG GGGGGEGGGGGDGGGGGGGDGGGGGGGGGGDGGGFGGGEGAGGFGGGFGGFGGFGGGGGGGGFGGGGGGGGGDGFAEA:CEEECEE9CCECABCBDDDCDD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:75:5406:10078/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGGEGFGGDGGGFGGGGGGGGFGGGBGCGFGGEEGGFEGGEEGCFFGFFE? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:39:9088:2610/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG ECE??D>A<>EEE:EDEEEEDEBBCE=EEEEE?DEEEEED@B=EADDEEEA;EEEDEEEE=EDD=EECCADDED?BAACDDB6?@?5DDBBEAE=EE=EAA PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:66:8142:4981/1 16 chrI 891 60 81M1D20M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAGGCAACCAAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGA >@DFGGE=EA:FEDFCABBE?DBEE?EEEEEFBCCFADFEGDFF?CEEEEBE?EEEDCAA>GFDEGEADEDFDFDAFBADFGFAEFFFEDEDDDEEFDFFE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:77:9937:16848/1 16 chrI 895 60 101M * 0 0 AAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAA BEEEEEEEEEEEEEEEEEDEGEEDFEAE<CCGFDEEFFGGFGEGGGGDGFGGGGDFFGGGFGGGGGGEGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:20:3429:5322/1 16 chrI 895 60 101M * 0 0 AAAAATTTGCAATTGTTTTAGCTAGGACACTTTTGGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAA ABGEGFEEAEECEFDFDEAFGFGGGGCEDEEEEE:DEFGGFF?FGFFFFFFFFD=FFGGGGGGGFGFGEEDGGEEGFFFFEGGEEEGEEEEECE??CEBEE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:106:10002:5503/1 16 chrI 896 60 101M * 0 0 AAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAA BBEEECEBGFGDGDGFGGAEGEDAB?EBB8DFD=EADDGGGDGEG?GFFGDFFFFCAFGFGGFFGGDDF:DADGGDFGGGGFFFBFGBFDGFFDDDEBGFD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:51:12803:14933/1 0 chrI 897 60 101M * 0 0 AAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA GFFGFGGFGFGGDGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGGGGGGGGGGFGGGGEAACEEGGEEFAEEEGEEEDEEEEABDDGDDECDDCDDEDG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:51:2990:9988/1 16 chrI 897 60 101M * 0 0 AAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA GGGEFGGFEGEFGFGFEFGGGGGGEGEGGGFGGGGGFGBGGGGGGGFGGGEGFGGGGGGGFGGGFFGGGGGGGGFGGGFGGFDGGEGGDFGGGEGGFGFGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:50:10205:11996/1 16 chrI 897 60 101M * 0 0 AAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA GFGEEEEFEEEEEEEGEGAGGGEEFEEGEFEEBGEGGFFEEGGGEGGGGGDGEDGGFGGGGGGGGFGEGGGGFGGGGGGGGGGGGGGGGGFFEGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:99:12968:10523/1 16 chrI 898 60 101M * 0 0 AATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAT GFBGGEGGEGFGGFGGEEDEE?B>B5>5DCCFE:AFGG:GGGG?FGFDGGGEGGGGGGGGGGGGFFGGFGGEGGGGDDGGFGGGGGGGGGGGGGGGGGGFF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:108:8721:8141/1 16 chrI 898 60 101M * 0 0 AATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAA D5:B=AFC?CF?FFFFE=FFFAFFDDDF=DFFFDFEFF=BFFFCFFFFFFFFFCFAFFFDFFFFFFABAFFADAEDFFFDFED?FFFFBBEDFEEDGFEGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:41:18519:16779/1 16 chrI 900 60 101M * 0 0 TTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACA BEEFFFE=BEEEBEEFEEAEEBD=>>EFBAF:FBFF:FFFFDDFDFFFFEEFFFFFFFEFFFEFEFFFFFEFFFAFFFFDFFFFFEDFFFEAEEEBEDEDE PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:7827:74939 165 chrI 901 0 * = 901 0 CATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTT CCCFFFFFHHHDFIJJJJJJIIJJJJIJJJIIIJJJIJIIJJJJJCGIAGFHIIJJJJIHHHHHHFFFFFDCDDDDDDEDDDCDDDDECCD@3?@:<CCC PG:Z:SNAP RG:Z:000 +EAGLE:2:43:17839:20176/1 0 chrI 901 60 101M * 0 0 TTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACAT ADEFAFEFFFFFEDFEDFDDFEEFFFFEDFFFFFDFFFFDFBFDEAFBFDFDFFDF=FFFFFFFABEBEEFF?DFFADFFACCD5@B?B=C:@B@AE;?:E PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:7827:74939 89 chrI 901 60 100M = 901 0 TTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACA DDDDDBDDEDEDEDDDDDDCCCCBBDEEDCFFFFHHHHJIJIJJJJIGHJJJJJJIJJJJJJJJJJJIJJJJIIJJJJIGIJJJJJJHHHHHFFFFF@CC PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:2:119:2085:12525/1 16 chrI 901 60 101M * 0 0 TTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACAT ?B?BBC@FFF=GGEFGACDD?ECACEFGEFGD=GEGEGGE?GGDGGGGGFGGGGGFGBGFGGFGDEFFDE?GEGGEFEGGGGGFGDGFGFGCCCEEDD<B= PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:37:2279:9702/1 0 chrI 902 60 101M * 0 0 TGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATT B><=??CCCAFDEFF=F?FFDF=D?AE?E@CFFABFEFFFEEE=C5@CA@FFFFFFFEE??BEDADA=DBEE?EBBEBEEDF>DBEEDEEEED:DEC@@5E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:41:11602:1908/1 16 chrI 904 60 101M * 0 0 CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTT EEEEEGDDEDBFEDBEDAE@CEEGEEEEGEFGGGGBGGFGGGGDGEGGGGGGGGFFGGFGGFGGGGGFFGGGGGGGGGGGGFGGGGGGFGGFFGGFGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:51:19603:11640/1 16 chrI 904 60 101M * 0 0 CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTT EEEEEED=DBBAEBBFE?E<?EEFGEEE?EEGFDGGGEGGGGEGDDGDDGGEGFGGGGFGGFGGGGGGGFGGEFGGGFGGGFGFFFGFGFFGGGGGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:22:13011:15244/1 0 chrI 905 60 101M * 0 0 AATTTTTATAGCTAAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTT FGGGDGGGEGGGGGFGGGGGFFGGFGEGGFGGGFGEGGGGDGGGGGGGEGGGGDEED=GGE=ECEGEFDBGFGEGE?DEDGEEFBEGEBDEEDEEEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:11:4741:6849/1 0 chrI 906 60 101M * 0 0 ATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC GGFGEGGEEFFGDGFFEFDGGGGGGDCFDFGEGGGFGGGGEGGFGGFEGEGGGDGFFFGDEEBEEDEEEEGFFGGFFGGD5EECEFGAFF?FECCCFGGF= PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:17:12718:17138/1 0 chrI 906 60 92M1D8M1I * 0 0 ATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAACTTTTTTCA GGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGDGFGGFGGGGGDFFGGGGFFGFFGGGGFGFFGFGGGFGGDGFGDFGGGGACA PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:84:4848:6681/1 0 chrI 906 60 101M * 0 0 ATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC GGGGGFGEGGGEGG?GFGGFFFFFFCCFDCFFDEFDECEECFFFFGGFFBGFG?CEACED5C@AAEEBAEEABEEEBBDDEEEEEEEEDEFEEEE@BBBDE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:70:5828:17291/1 0 chrI 906 60 101M * 0 0 ATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTC FGGGDGGGGGGDGFGGBGD?FFFBFEGGGGGGFGFFFDFBFFEFFGGGGGGFGGAGGGG==EBCBDFFEEGFEEEGEBEFAEEBAEEGEGFGEGA@EDBED PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:88:11351:17404/1 16 chrI 906 60 101M * 0 0 ATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC E?BBEBABBFEEEEEBGBCCEFEFF?AGGFEDEEFGGFGDGGGEGGGGFFGFFFEBGGGFFGGFBGGGGBGEGEFGGGGGGGGGGGBEEFEFEFFFDGGGF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:21:9227:5397/1 0 chrI 907 60 101M * 0 0 TTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCG GEFEGFGGGGBGGDFGFFGGGGGGEGFFGGGGGGGGEDFGFF=BFBBDEEDFGCFBFEF?D@CEEECCEEB=EEEAEFFFC?BB:BFEFDC<6>8DDEDDF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:96:7398:5258/1 0 chrI 907 60 101M * 0 0 TTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCG EGGGFGGFGGGGGFGGGGGGGGGGEGGGGGGGGDGFGGGGGGGGGDEGDFGG=GGGGGFEGDGGGGGEFGEGGGGAGDFFGFEECGEDEGGEEEEFGFDDG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:59:12995:3551/1 16 chrI 909 60 101M * 0 0 TTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGG GFGFEFDFEEEEDDCCCEEE?GDFEFDDBGEEGBGGGGGGGFGGGGFEGGEGGGGGGGGGGGGFGGGGGGGGGEGGFGGEGGGGGGGGFGFEGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:80:3409:18738/1 16 chrI 912 60 101M * 0 0 ATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTT EEEEFEDEBAFA@@DEEEEEGEFFGEGGFEGGGGGGEEDGGGGGFGEGGGEGFFGEGFGGGGGGGFGGFGGGGGBGGGGGGGGGGFEFFFEDFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:77:1620:5914/1 16 chrI 913 60 101M * 0 0 TAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTT ??@@??<A=?<??CCCEEEEEE@C=EEEEEAFEGGGEGGEEFGBGGFGGGGEGFGGGFGGGGGGGGGGGGGGDFGFGGGGGEGGGBFFFFFF:GGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:72:16563:11844/1 16 chrI 915 60 101M * 0 0 GCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTT AECCB=DDA=CED?DFEEAE:C??A?5EBEEBFFFF>FFEFE?EDE@>ACCAEFFFEEEEE=FFFFE:EEDDFEEFEEEEDDDDDB?:>>?ACC>C?DE?= PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:103:12336:20768/1 0 chrI 917 60 101M * 0 0 TAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGA FGGGGGGDFGGGGGGGDGFGGGGGGGGGGGGGGGFFGGGGGGCE=DGGDGGEGGDFA?EFGGBEDEGGGFEG?@GEE?FFBGGGDGFBGGBFF?A:A>;@E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:33:12668:17941/1 0 chrI 918 60 101M * 0 0 AGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATTATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAA FGGGGGGGGGGGGEGEGFEGDGFFDDBA?FFGDFGGGECDGFDFFFGE=G@EEDEE=BEE=EEECE?A:F=DEDD:E?EEC;@;DDEEADBE5@BEEB:5F PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:70:1514:14146/1 0 chrI 919 60 101M * 0 0 AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAA DFAAFFDFFEEEC>E=DDDDDEEEDDEDEE?DEEEE?EEE@6CBBEABEB=AAAADBEEBEEDEDEE:EED@BE=AB?B8C?=EEEEEEEA<:@B?????: PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:41:3640:5327/1 16 chrI 919 60 101M * 0 0 AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAA 5FFF?CFFFFDEEDCE5=DE=EEEEDEEEEB?>@@DAEEDDBFAFFDDDD?DEFFDFFF>FEEDBEDEBFFDDDDD=C:A?FFDEFDEEDBEDEEDBBECE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:70:16168:12341/1 0 chrI 920 60 101M * 0 0 GACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAAT GGGGGGGGGGGGFGGGGGGEGGGGGGEGBGGGGGGGEFGFGGGGGFGGEFGGGF=FFDBFFGCEGDEGFEEDBEGEBEE=@EGGEEBEFE==BBEDB:DAD PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:6:3780:20120/1 16 chrI 920 60 101M * 0 0 GACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAAT C=B<??FEEBDG5DEGABCFGEB?FFFG?BGGGGFGFDFEFEEDD?FGGGGGGAGGFBGGGFGGFFFGFGGEGDGBGGFGGGFEGGGGGGGDGGGGGGGDE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:38:5564:3174/1 16 chrI 921 60 80M1I20M * 0 0 ACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAAT AEAA?DGEDDEGCEGDFGGGEGDGGDGGEFGGGGGGGGFGFGGGFG?FFFBGEGGDGEFFBGGGGGFFFEEGGGGGGGGFGGGGGGGGEGEGDEF?GGGGD PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:44:6557:3548/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA GGGEGGGDGE=FFFEGEDFFCFEECGBDEGGGGG=GEEGGGDGEEE?EEEGGEGGEEFGGEFBEEC?A5CEDA=BEEE8EBEDADEEBAEBBBCC5@@ABD PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:23:12527:20440/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA GFEGGGEGFGGGFGGEE:DDEE=EEGEGGDAFFA?GEGEGGGGDGGBGEGGG?GGEGGGGEGFGGFCGGDDBGBFF>BD@EGEGEFDFC=DDDEBD5?DBE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:98:18353:7963/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAATCATTTTTCGGGTTTTTTGAAATGA @@C@>ACC:9>D:BDC:DD:DA?ABD?DDDD?BDDCC@>A?DDC5A<5A?BB?BBA=ACA@BBBBCC???@@??@A?AABBBBB@:=?8ABBBBB@@BB?? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:84:5126:1325/1 0 chrI 922 60 76M1D25M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA GGGGGGGGGGGGFGGGGGGGGGGGGFGGFGGGGGGGGGGGGGGGFGEEGGGGEEGFEGFGEGGFEEGEGG@BAEEEEEEEEEFEECD@9CCDDD=EEEE?? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:12:11478:10672/1 0 chrI 922 60 76M1D25M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA CAC?=:BBD;EFDFEFFDDFADFFEFFFFEFEFFDFCC=EFFD?EEBA?DDFCA;AEEB:DBFDEFEEBADBBDEEDF5=BEE?E?B=@B@BCC@B=ACB? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:95:5316:12215/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA GGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGFBGDGGGFGDGGGGGEGGGGGEGGFEGGGEGGGEGFEGEEFBEEEC=;CEEGEEEDFA@CCCE=C=AEED PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:113:5213:14267/1 16 chrI 923 60 75M1D26M * 0 0 ACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAAT =:;AABB??EB?B=DDA:EEEDDDEBB?EEEDEGGGEFEEGFEDC@@EFEFGGEEGCDB?ABA?5ADDGGGEF?AFGFGGFDFABFBDDGGF?GGGFGG5F PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:118:8721:7001/1 0 chrI 924 60 101M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT GGFDGGGCGGFGGFGGGDGGGGDGGGGFGBGGGGGADFFGGGGGEGFDFGEEGBACECBEBADFFEEBGEBBDBAC@CGGDBBDGB>EEEDFDDF@E:D@8 PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:79:1079:9916/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAATTTTTTTCAGGTTTTTTGAAATGAATA ;@EAEEEEGEEEEBEGGEGEGGAFEE@:@:;FEEFDEDEDGEGGFGBEBGGBGFGGGEDBGGEFGGGGGDGGGEFGEGGGGGGFGGGGDFFGGGFFGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:85:1256:19207/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGTCTGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCGGGTTTTTTAAAATGAATA ECEEC=FEFGDEGFEDCGFDFGFBGEGEEEGEFBGEEGDGFEEGGGGGBGGAGGGGGGBDGFGGGGGGGEFGGDGGGGGGGFGGGGGGGGGGFGGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:48:19056:2032/1 16 chrI 924 60 101M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT C;BAAAB:EDDEGEEEDAE@EDGGGBG:GDEGGEFEEFAGEAGGBGGFCEEGFDGGFFGFGGFGEFEECCAEDE@EGBFDGGDGGGGGEAEGGGFEGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:76:11456:6131/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA EEEEEEGGEEGDGGEEDGGEFFGGEEGEGEGGGEEGEGGGGGGEGEFGGGGFGGGFGGGGGG?GGGDGGGGFFDFFGGGGGGGGGEGGGGGGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:61:3268:15848/1 16 chrI 924 60 74M1D27M * 0 0 CTTTCTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA FEEDFAGDGEFEEEEAEGDEGGEGGGGGFGGEB=EACEEEBDGGGGEGGFFEEEE?DGBBFGGGDGGGGGGDDCD;DFFF?GGGGGDGGEGDGGGFDFFGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:86:4115:1680/1 16 chrI 925 60 101M * 0 0 TTTATGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTA ?AA9BACFCCFGEEGFCFDFEEGDEGEGGGGDDGEEEDGGGFGGGFGDGGGGGGGFGGFGFGGGGFGGGGGGGGFGGFFGFGGGGFGGGFGGGGFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:2:10237:11117/1 16 chrI 925 60 101M * 0 0 TTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTTAAATGAATA @:FFFFFDE?FFAFFFFFDDDDFEFFDADEEBFEFDBD?FFFFE?FFFDFFDDFBFEFFFFEAADEC<CCBEE:DEFFEBFFFEDDA?FFFBDDDDD=DCB PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:85:13088:19333/1 16 chrI 925 60 101M * 0 0 TTTTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATA :?GDBEEGBEEEDGEEEEEEEFEEEFGGGFFFEDFEGDGGGFGGBFGGGGGGGEFGGGGGBFFFEAGGEGGECEEBEAGGFDGGGGGEGEGGGGFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:79:18345:10394/1 16 chrI 926 60 101M * 0 0 TTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTAT EBBBCBEEBBBEGBGEFEFDGBEBD>DGGGGBEFAGEFFE?GGEFGEGGEGFGFGGFFFFBFGDGGGGGGGE=EDEEEGAGGGFGGDFBBFGGDGGGGGFF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:11:6740:20191/1 16 chrI 927 60 101M * 0 0 TTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATC AA?@?BAAB=A?::::??B?AB>E>??@EAA@:@?<@>>==@@:CA=D?DBDBB@EEGGEBGEEEE?AEBAADDBBGDBGGGEFGGBGEGGEEEEE5DABD PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:39:17435:10955/1 16 chrI 927 60 101M * 0 0 TTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGATTTTTTGAAATGAATATC E=D@DFEE=:?GDEBFEEEE?E=ECCBEGDBGGDFBGGGGEFGDGFEEBEAFGEGGGGGGGDGFGAGGGFFEGGGDFGGDGEEEE:GFBGGGDEGFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:83:18991:16122/1 16 chrI 927 60 74M1I26M * 0 0 TTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATAT EABA5?DEFFEEC5@BDDFEFDFECCFFFFFACA:?=EFFFEBDEAFBFBBEE=DEAAF=FBDDA>F>BFFEEEED?DD5?GGGGF=EBGG?BGFFFEFFB PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:23:6768:7044/1 16 chrI 929 60 101M * 0 0 TGCCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCAT FBB??E?CB@??C=B=C?=?:E=CCD@AA?:5E5EDF=FFFDFFFBBEBEEEC:EEEBCBAFFEFDDFDDFADEEFFBFDFFEFFDFBBEFGDGGDAFFDF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:47:19241:12210/1 16 chrI 929 60 101M * 0 0 TGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCAT GGGEGGEEGFEEGGGFGGGEEFGGEGGGGGFGGGGGFGGFGGGGGGGGGGDFGFGGGGGGGFGGGGGGFEFGGGGGGGGGGFGDEEGGGGGGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:79:9772:3464/1 16 chrI 931 60 101M * 0 0 TCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAG =ECCBB=?FGFG?GGGGGBGGFEGDCBFFF=EFBFGGFGGGDFGFFGGGGGEFGGGGGBDGCCCEEFEFFEFFEFDGGGGEEGGDGGGGEGDFGGE?GFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:29:4194:2200/1 16 chrI 932 60 101M * 0 0 CTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGC @:AE5DDEEBE>@@=5BBB8G@DFDFGGEEBEE=BB=GGGEEEEDEFEFFFADGBGEGGGGFBFGAGFGGGGGGDGDEDFGGGGGGGGGGGGGGEFG?GGF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:32:7810:14929/1 0 chrI 933 60 101M * 0 0 TGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCT GGGGGGGGGGGGGFFGEGGGGGGGGGDFGGGGGGGFGGFGDFFDEGEGGEGGGGGGGFGGGBEGGGECGFGGGFGGGB5GFFFFA:CEE:B5?B?FDEAFE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:38:7229:15314/1 16 chrI 933 60 1I65M1D35M * 0 0 ATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCT G?EFEDFE=FFFDGFGGBFGGCFDBAGG=FEEGDGFGGGGGFGGGG?G?GBEFEAEGGGGGEEEAAGGGDGGGGGGEGGEDGGGGGFGGFGGGGEGGGEGE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:45:5717:2359/1 0 chrI 935 60 101M * 0 0 CCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTAC GGGGGGGGFDFFFGDGGGGGGGGGGGGGGGGGGFGGGGBEGGGGGGGFGGEGGFEDGEDEFGGEEFFGEFGGBBGEEEEBEE:CD:AAAADEEEEE?CCC? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:67:2263:13075/1 0 chrI 935 60 101M * 0 0 CCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC EDBEEEEEE?E?EEDDEEEEDDEECEDEEA>6C<@>BB>9?ACAAEECEE?CCEE?C:=B5AB??AEEEEB@DDD:>>@@C:?@??@:?@BCAC@@?A@?< PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:94:5592:18698/1 16 chrI 935 60 101M * 0 0 CCCATATATCGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC ECECBEABA9AAEE@C;EDGGFEFGGGDFGFGEEEFFFFGBGGGGGFEAGCDGGFGGGGGGFGEFGGGGGEGGGEDGFGGGGGGGGGGDGFGFGDFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:59:10637:1223/1 16 chrI 936 60 101M * 0 0 CCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACA CAEBDCA?EECEDDF@@GGGEGEEEEDEGEEFGGGFGEGFEEGGGGEDDE:FFFDACECDFEGFCGGFGFGGGFGAGGGFGGGBGGFGGDGGGGGGFGFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:70:4295:15837/1 16 chrI 937 60 101M * 0 0 CAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAG FCCBCB=CECBEEFEEGEGGEGEEEEGGGGEGEBEEEGGFDGGGGGFDDGBGGFFGGGGGFBGGGGGGGGGGGGDFGGDFFGGGGGGGG?FEEEEDBEEEE PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:64:5817:4431/1 0 chrI 938 60 101M * 0 0 AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA GGEGBGDEEGGGGGDGGGDGGGGDGFFDFFGFGGGGGEGGEFEFDGGGGGDAFG?BEEBBFGFGGGGGGGGECGFGEFAEGGGF?FEF:A@?@C:?:@@@E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:8:6755:19986/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA BBEA==FEBAFFEFFFDADEE=D=CEE=D=;FFFDDFFFDEEEFFA=FB=FEFFFFEFAFFDDDFEFFDFDFFFF?DDFFFEDFFDFDFEFCDDECD:DAD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:105:14800:10824/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTGGCTACAGA :GF=EEGGECDEFFGGFGGGGFFBEDDEF@EDGEEGCECEEE5EEEEFEF=FFGGDCGGEEGGDGGEGGGGGGGGGGDFGFGGFGFEB?EEE:EEDFFFFF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:71:6398:5629/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA BCCAC:DFD?FF?>ECDDF?DEF?=FF?F?FFF?BFDFD?FDEEA=B??EE?E:B>@ACC5EEEEEDDBDFBDEBDEEDEDEEEE?ED:BECABECBBDA: PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:84:15268:14389/1 16 chrI 938 60 101M * 0 0 AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA D:A@A?5:BCA?FCCCABDEEA@@5BFBAADEEDFFEBBBCEBEEED=5DEDDCCAFFFEDDADBD=5FFFDED=AEFDFFFDFFFF=FDDFAFFFEE:EE PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454 99 chrI 939 60 100M = 952 113 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA CCCFFFFFHHFHHJJJJJJJJJJJIJJJJIGJJJJIJJIJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFBDCD@BDDDDDDDDEEECBDDDBDEDDDD PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788 73 chrI 939 60 100M = 939 0 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA @@@DFFFFFHHHFFHIIFGIHIIGJJJIGFGHGIGDGG9DGIJBHIJJIIHGHGHCG?A@BGDAECHFFDB;?CDDDD@CCDDC:CAD:?CB<<CD>AAA PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788 133 chrI 939 0 * = 939 0 TGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATT CDDDDC>D@@<>@ADCDEDEECCDBFFFHHHIIHHGBIIIGEHAIJJJHFJJJJJIHFFAJJIHCEIJIIGJJIIIIIHGHEBIIJJHFHHHFFFFF@@C PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489 99 chrI 939 60 100M = 951 111 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA CCCFFFFFHHGHHJJIJJJJIJIGJIJJJHIJJJIJJIJJJJJJJJJJIIJIIJJJJIJJJIJGHHGHFDFADDDBDDDDDDDDDCDDEDCDDDDEACDD PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:25:14090:18624/1 0 chrI 939 60 101M * 0 0 TATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAA FCFFFGGEGGFGGGGGGGGGGGGGGGGFGEGGGDBEGGGGGGDEGGGGGGGGGGGGEFFEEEEFE?EEDEEC:EBECEEEEFGEDDFE?FBEE@DDDDD=F PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:33:5876:3688/1 0 chrI 940 60 101M * 0 0 ACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA GDBGEFFGGDGGDGGGDFBG@GGFGGFEGFFGGEDGGFDEGGFEFBFFFBC?E=CB?ECAGGG=DGGFFFEFGFGGG??GE::55BCAEDED?EC5D5CC9 PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:55:14770:18631/1 16 chrI 940 60 101M * 0 0 ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAA FEGBEEEGEEFG@GFGGFGGFGEGGEFGGEFGBGEGGDGGGGFFGGEGGDGGGGGGGEEGGGEGEGGGGGGGFGGFGGGEGGGGBGGGGGGGFDGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:93:9444:3777/1 16 chrI 940 60 101M * 0 0 ACATAGGCAACCAAAAATATTTTCCAAGTTTTTTTTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA FFGADEGFFEGGEGGBGGFGGGGBGEEGGGGGGGGGFGGGGGGBGGGGDBFFDGGGGAEGGFGGGFGGGGGEGGGFGFGGGFFF=FDGGDGGGAGGGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:50:5922:5719/1 16 chrI 940 60 101M * 0 0 ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAACATCGTAGCTACAGAAA :???:???5:==8@EECCC5CA?5ECACCCEFEEEEEEFGGGGDEEEGEEEGEEEEEEEGGFGGEEFGGGGEGEGEGGGGFBAAD:GGGGGGGGFFGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:21:17413:6903/1 0 chrI 941 60 101M * 0 0 TTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAAC EEBAD:E?DAFFF:FDDEBEGGGDGDGFEFGFGFGGFDDGEEGGGFFGGGGGDG?CDGGGFDFFGEEEEACEC=EEFGGFBG?G?FDGF=?A=D=FFF5F5 PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:120:7503:14055/1 16 chrI 941 60 101M * 0 0 CATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAAT EEDEB5GFEEGFGGGG:=GGFDDFFFAA7EEG:BBGGDFEGGADG=GGGBGGGGGGGEGGFGGGFGGFFFEGEGDGGGGFGEGGEGGFGGGGGFGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:91:17352:13444/1 16 chrI 942 60 56M1D45M * 0 0 ATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACATAAATGG @CCBCDE?EEEEEEAEEEEEEGBEE>EEDEEGDGEBFEEEGGE?EGFFGEGFFFECFBEFGDFGGGGGGGGGGGGGFGGGGGDFGGFGGGGGGGGEGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:97:3188:16851/1 16 chrI 943 60 101M * 0 0 TAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGG :B??BB5=8CCEEEEADABDECB:EA@=?BB5C>C;:DAA::A=?D:ECCECEEEEC??C???=D?C??=?DB==?D?BDD:E=AEEEEAEDC?C?DDDDA PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:85:10558:18446/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG FGGGGGGFGGGGGGGGGGGGGGEGGGGGGFGFFGGGEFEGFGGEGGDGEFGGGEGDGGGCGGGFADEFFFFEBFEFBEFFEEGEGDBEEEDGEEEDDE?=A PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:35:15009:8527/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG GGGBGGGGABGG@EGGGGGGGGEGFGGGEGEFGGCGEGGGFEGGEGEFD?FGC=CEEGGEEDFE?DDFEFAACE=ECCCC?GDEEEEBBCEE@EECCB?CB PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:62:14360:14144/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG GGGGGGGEGGFGGGDGGGGGEGFGGGFGGFFFGDGFEEFFGGGFEFGEGGGGEGEDGGDEFFFFADEFDEEEEED?EEEEEEGEEDEEEEEEEEEABB7AA PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:81:13994:10137/1 0 chrI 947 60 51M1D49M1I * 0 0 CAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCAGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTA EEEEEB?EEDBFFFFFFFFFFBFFFFFCCCABCC5?DDD?FEEDFEFEFDBFEEFFFFFDFFEEEEFFEEBEEEEDDEBEEEEEEEEEEEEEEEE@?@D?@ PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:69:16589:8289/1 16 chrI 947 60 101M * 0 0 CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT @A???;>BA@DB:DA=BBB@@AEEDEADEEEEFBEFDFDDEBFEEEAAEBB?FEDFFFEFFFDFFDE:FFFEF?FFFDBFFF?EFDEEEEEE=?DEFDFFF PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:46:11875:10933/1 16 chrI 947 60 101M * 0 0 CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT =EBDDGEGEGDFGBEEGBEGGGGDFDGAGGGDGGGEEGBGGGGGGGCE?EEAD=D?GGGFGGGFFDGGGGGGFFGGFGEFGGDGGGDFGGFFGDGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:70:7204:17965/1 0 chrI 950 60 101M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACA GGGGGGGGGGGEGGGFBFDFGGGFFDFAGGGBEGGGEFDFEGEEGEGEEE@EFFFFGGGEEGGEEDBDEEEEEEDEEGGEEAB=AEEE@EDD?D@?B9@B= PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:47:16300:14806/1 0 chrI 950 60 48M1D53M * 0 0 CCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACAC GGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGFGGGGGGGGFGGFGGFGGGEEGGFGGGGGFFDFEGGEGFGDGEGGGGGGGEBFAECC?EEA PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:72:3307:18932/1 0 chrI 950 60 51M1I49M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC EEDEDDFFEEBGGGFGGFGGGDFGGFGEGGGEDGGEEGEGDFFGGEEGGEGEEFGGGAGFFADFFCEB?CEEDEDDCBCEBDBCCCECEACCACE;??;:: PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:55:6957:9011/1 16 chrI 950 60 101M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACA :A??BB?AAB=?=BBBBCCDC?BDDDBCDBBCDB?DB?:DDADDDDDD@?=DADD?DDDADDDDDDCDD:DDBD?:DD?DD?DDDDADDD?ACA>ADDD?: PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:41:5354:20962/1 0 chrI 951 60 101M * 0 0 CAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC GGGGGGGGGGGGGGGFGEGGGGGGGGGGGGGEGGGGGGGGGGGFGGGGFGGGGGGGFGGBEEGEFB=EEEEBED=BBCABEBEBEEEEEEAAC<=A<:@AA PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489 147 chrI 951 60 1S99M = 939 -111 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC #DDEEEDCADC@CDDDDDDDDDCDDDDDDDDDDEDEDEEEDEDFJJIHD@IIJJJIJJJJJIIGJJIJJIFGJGIIIJJJJJJIHGJHHHHHFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:2:48:13688:1594/1 16 chrI 951 60 101M * 0 0 CAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTGCAGAAATGGTTGTACAC @EEEEE?BEEEADBAC:EEEEEEEEEECEBEECEEEBEEDEEEEEE>>A@=BFDEFGGEEFGEEFEFGGFEFFGGFGGGGGDGGFGEFFFFAFFBFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903 165 chrI 952 0 * = 952 0 CTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT CCCFFFFFHHHHHJJJJJJJHHJIJJJJJJJJJJJJJJJJJJJIJIJJJJJJJJJJJHHHHHFFFFFFEEEDDDDDDDDEEEDEEDDACDDDDBDDEEEA PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230 165 chrI 952 0 * = 952 0 CTTCTTAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFFFFFEEDDDDDDDDEEEDEEDDCDEDDDDDDEEEE PG:Z:SNAP RG:Z:000 +EAGLE:2:94:15425:16067/1 0 chrI 952 60 101M * 0 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACT FEGFGGGGFFFGGDGGGFGGFGFFFEGDFFEGGGDEEDDGGFDFGGEEGGDEGFGGGGBDEFFFFEBEEGBEEEEEGEEEEEEFEGDEEEBE;EE?BCCCC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903 89 chrI 952 60 100M = 952 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC DEEEEDDDDDDDDDDDDDCDDDDCCDDDDEEEEDEEFFFFFHHJJJIJJJJGJJJIJJJJJJIGHJJIHGJJJIJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454 147 chrI 952 60 100M = 939 -113 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC CEEEEDDDCCCDCCCBDCADDDDDCDDDDCDDCEDDDDCCDFHIHHCHJJJJJJJJJJJJJJJIIJJIGIJJJIHIJJJIJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230 89 chrI 952 60 100M = 952 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC CEEEEDDDDCCDDDDDDDCCCDDDDDDDDDDEDEDEEDEEDFHJJIEIJJJJJIJJJJJJJJJIIJJJJIJJJJJJJIJJJJHJJJJHHHHHFFFFFCBC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:48:14890:12005/1 16 chrI 953 60 101M * 0 0 AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC CEDF@C@EE=EEDEGEEBGEEEEDEBEFB?BEAEE?FDCC>CFFDDG=GEGFCGGGGGFEGDBGGGGAFGBFGAGEGEGFDFGGGGGGGGFFEGGFGGGGE PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:67:18282:4840/1 16 chrI 953 60 4M1I15M1D42M1D38M1I * 0 0 AAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT >A9EBCDAD@@EFDEFBDFGFGEGGFEEE?EGFFGGEECCC@?:BAFBFDFGGGFGGGGGFADFGGDGDGGF?FFFDFFDFEEEDDBEEEBFFBFFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:50:13587:7527/1 16 chrI 953 60 101M * 0 0 AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC GEFEEEEGEEFEEBDGEDGDFGGGEEGFGGGGBEGGFGGGGGGGGGGGEGFGBFGGGGEGGGBGGFFGFGGFGGFGFGFGGGFGGGFDGGGFFFFFEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490 165 chrI 954 0 * = 954 0 TTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATGCAA CCCFFFFFHHHHHJJJJJIJJJJJJJJJJJIJGIJJJJJIJJBFIIJJJIHHHHHH@DFFFFFFEEEEDEDDDDDD@BDCDDDDDDDDCDCDCC@>::>C PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020 165 chrI 954 0 * = 954 0 TGGAAATTATTTTTGGTTGCTTATATTTGGGCAGACAAAAAGTGTCCTAGCTAAAAAAATTGCAACTTTTTTGTATTTTCAGAAGAAGAGTGTACTACCG EEDEDDDDBDBDDDDDCCDDEEEDCBCCCEEFFEDFHHHGHIIJIJJJIIGJJJJJJJIGGGGGFIIEJIGDGJIIIIHEFJJJJIIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:2:71:10251:4806/1 0 chrI 954 60 101M * 0 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT GGGGGGFGGGGGFGEGGGGGGGGGGGGGGBGGFGGEEFDECEGGGEEEGGFGGGDGCCFEFGEDDEEGEEEEEEEEEDEE@GEEECDCDD?CC>CCAACCE PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490 89 chrI 954 60 100M = 954 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACAATC ::>C@CA>:CCCDDDDDDDDDDDDDDEEEDCFFFFFFFHJJJIHCJJJJHJJJJJJJJJJIIIJJJJJJJJJIJIIJJJGHJIIJIHHHHHFB=24=+B= PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020 89 chrI 954 60 100M = 954 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC CCDC@A@@DDDDCDDDCDDCDCDDDDDDDEEEEDEEFFFHHHJJJJIJIGJHGJIIJJJIJIIJJJJJJJIJJJJJIIJIJIJJJJJHHGGHFFFFDCC@ PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:2:50:6355:12073/1 16 chrI 957 60 101M * 0 0 TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT ?C=@CA=EDCDBEGEEGEEAEDEBEFDFBCEGEEGGGGGGEEFFEEGGFGGGGGGGGGGGGGGDGGGGFDGGGGGGGDGFGGGGGFEEEEEFGFFGGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:65:16283:15152/1 16 chrI 957 60 101M * 0 0 TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT EEEGEEEEBBDGGEGGECEE:BFGGGFFGEGFGEGDC?EEDCEEEAGEAGGGFGGGGGFGGGGGGGGADGGGFGGGGFFGGGGGGGGGGGEGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:79:12640:7363/1 0 chrI 958 60 101M * 0 0 ATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAACGGTTGTACACTTTTCTG FFFEADDDDD?=DD;A:::C=ADDBB?DCDEDFFD?EDEEAAEEAFDFDD=C?:CD?DBAEBEEEF=FAAEEDEE=CB=BBEBBBDBDD?ED@B@D@ED@: PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:65:16789:19099/1 0 chrI 959 60 101M * 0 0 ATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACACTTTTCTGA EEDDDCB?DBACCCCCC>EEEEEAEBBDDBEAEBCDEEDACEEEEEEEEEE@EEEEEBBEE?EAEEEEEBEEEEEEEEEBDEDEE@BDBB@B@@BBD@<DB PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:42:12059:15505/1 0 chrI 960 60 101M * 0 0 TTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAA GGGGGFBFFFFBDFEGGF?GGDGEDFGEDFFFFDFGFGGDACEFEFFFFFAFEFFFEFGGEFG:DB=ECBBEEBBEEEDFEEEA>>=8>=A@@@AE@==BA PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:56:5751:3283/1 16 chrI 960 60 101M * 0 0 TTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCT AA@ABA5AFFFFBEEGDDE=FEF?AF?EEDA>>@>EEFAFCCCCC=DEG?FGEBFAGGFBGDDC5C5DFFD=GDGGEEEDGGGGBGGGGFFGGGDFGGAEA PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:7:4422:19468/1 16 chrI 961 60 101M * 0 0 TTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAA GBEEE?DBFFFGG:GGEGFCGGDECBFBFFD@@>D5ECEEAGEFGGGGGGAFG:GGEEEEEAFFFDGGGGGEE=EDGGGGGGGFGGFFFBFGGEGGCCGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:24:3469:6979/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA EBB=BDGFFGFEFAF:GGGEGFAFEBDDEGGGGGFG=DDDGGGGGGGEFGCFFFGFGBGGGGAAFGGDGFGEGFGGFGGGGDFGFFFBFFAGGGGGGFGGD PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:107:10805:16122/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA DA?C=EFABFGEC=DBEFFCFCCBBCE=ECCCCEFE:FAFGGGEEEEBDDEGG:EEFGEFG?AFFFFFFEF=FGGGGGGEGGGGGGFAGGGDGGGGGGBGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:103:2608:5570/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA GEFDEBGFGGGDEFFCDEDEAGGFBG=GGGGCCFDEDFCBFGGGGGGGDGGGGGGGGGGGGGEGDGGGGGGGFGGGGGGFGGGGGDGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:103:8177:14669/1 16 chrI 963 60 101M * 0 0 CCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAT EGBFCEEFAFEEEEEGGFEFGEEEEEGFEGGGGEEGGEEGGGGGEFEEFGGFGGGGGGGGGGEGGEGFGGGEFFFFGEGGGGGGGFGGGDGFDGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:77:9271:19697/1 0 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA GDGFDFGGGEFGFGGGGFGGGGGGGGGGGGGGGGGGFGGGFDGGFFCFGFGGGFGGFFGGGGEGGEGGGEDGFEEEEFFDAEFCDGEGBEEGEEFEEEGEG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:88:12652:14437/1 0 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA EEDEBEDEDEF=FFBFCFEAFFBFFFBFDF?DFFCE@=FDBBFEEC?BEAFEBEBFEEEEDEEFEBBEF@BE?ABAEDDB5==6=<@?@@@@=@B@A:AAE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:66:2841:12985/1 16 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA EBEEGGG=DFEGGEFDGGGGGFEGGFDFGGCFFE?FFGAGGGGGGGGGGGGFGGGGGFGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:47:10826:17209/1 16 chrI 965 60 101M * 0 0 AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATAC EDDDDBEEGEEEEGEFEGEG?EEFFFEEEGGFECGDGBEGEGGGGGFGGGFGGGFGGGGFEGEFFGGGGEEGGFGGDGGFGFFEFE5GGGGGFGGAEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:42:13795:17461/1 16 chrI 965 60 93M3I5M * 0 0 AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAA C?CFDFBGEEFE<GGGDDECDAGBFE@CCCFE=EGEFEGGBGGFFGGGGEGGGGGGGGFGFFDGDGGFGBGGGGGEGGGGGGFGGFGGGGDGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:64:12381:18587/1 16 chrI 966 60 92M3I6M * 0 0 AGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAAT EBBEEEGGDGGAEFEGGGGEEEGGFECCCFEC=EBE=GGGGGGGGEGGGFGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGFGFGGGFGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:21338:23882 165 chrI 1001 0 * = 1001 0 TAGCTACAGAAACAATAGAACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAA >==AA>>AB4=AABCBAB+<3<ABCCB7?+A@A<ABABBBAAABBB>BB7AAABAAB78)9)3=BAABABBAAAA9;?9;;.;>@?B=;=??>:=199=9 PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:21338:23882 89 chrI 1001 60 46S53M1S = 1001 0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTCGNGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCN ##############################################@;C<-0(#??)<????HGD<CFFCE?CACE9B<HGICHEHGFFDHFDDBB:11# PG:Z:SNAP RG:Z:000 NM:i:3 +EAGLE:2:100:13144:11902/1 16 chrI 1359 60 101M * 0 0 TTACGTAATAGCTTCTTTGACATCAATAAGTATTTGCCTATATGACTTTAGACCTAAAATTGACTATTAATGCCAATTTCATGATATCTAGCCACTTTAGT EAAB?@=AB:BBDDDDA@?==@ABE=B:EBB?DE=BFBDBDFDEBFEBGEEDGFAFFAFFBDDFE=GFGGGBE=EAFE?GGDGDFFEFBBGGGDFGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:48:18830:9303/1 16 chrI 2070 60 101M * 0 0 TTTGTGGGGAAATATATGTTTCTGACTCTAATTTTGCCCCTGATGCCGAATATCGATGTGAAAAAATTCAAAAAAATTTCCCTGATTTTATATTAATTTTT CCDD=FEEBEEEEEEEEEFEEEEFBDEEEGEEEEEGGBDGFGEDGGGGCGGEFGGGGGDGGGGAEGFFFGGGGGFGBGGFGGFGGGGFGFGDGFGFGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:28:7318:20945/1 16 chrI 2075 60 101M * 0 0 GGGGAAATATATGTTTCTGACTCTAATTTTGCCCCTGATACCGAATATCGATGTGAAAAAATTTAAAAAAATTTCCCTGATTTTATATTAATTTTTAAAAT CEEEEEDDBDCADB?DGEEEEFBGGDGEBGGDGFGFGGGFEGFGGGGGGGGGFFGGGGGGGGGGGGFGGGFGGFDGGGGGGGGGGGFDGGGGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:74:12831:9830/1 16 chrI 2094 60 101M * 0 0 ACTCTAATTTTGCCCCTGATACCGAATATCGATGTGAAAAAATTTAAAAAAATTTCCCTGATTTTATATTAATTTTTAAAATACGAAAATCCATTGGATGC =CC=E=GGGEGFFBEDDFFFAFFF?FDFGFGGGGGGEFDGDEDGGGGGGGGFGGGDFGFGGEFDGEGFGGG?GF?GCCCCCD8DDDGFEEGEEEFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:26:9616:12584/1 16 chrI 2512 60 101M * 0 0 TATCATTAATAATATTTATCAATTTTCTTCTGAGAGTCTCATTGAGACTCTTATTTACGCCAAGAAATAAATTTAACATTAAAATTGTTCATTTTTGAAAA =FBEEEEGEEEE?EDEDFEDEEA?EEFGEGFEGEDEDFGFEFDGGGEGDEEGGFGEDGGGFGFGGGGGGFGGGGGGGGGGGFGFGGGDDGGAGGEEGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:10647:87203 73 chrI 2547 60 71M1I28M = 2547 0 GTCTCATTGAGACTCTTATTTACGCCAAGAAATAAATTTAACATTAAAATTGTTCATTTTTGAAAAAAAAAATAATAAAAAAAACACATTTTTTGGAAAA CCCFFFFFHHHHHJJJJJJJJIJGIJJJJJJJJIJJJJJJJJJJJJJJJIJJIJJJJJJIJJJJJJJJFDDD?CD;ACDDDDDD<B8<>CDECDBBDDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:10647:87203 133 chrI 2547 0 * = 2547 0 AAAAACACATTTTTTGGAAAAAAAATTGTCCTCGAGGATCCTCCGGAGCGCGTCGAATCAATGTTTCCGGAACTCTGAAAATTAAATGTTTGTATTATTG @DCC@C@DDBDDCDEDDDDDDDDDDBDDDDDDDDDDDDDDDFFHHHIJIIJJJJIJJJJJJJJJIIJJIIIFJIJJJJJJJJIJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:1:84:3615:2746/1 0 chrI 2728 60 101M * 0 0 TTAAATTATTGTAGAACCCTTTCGTTATTGAGATTTGATAACTTTTAAGTAATAAAATTTTCGCAGTAAGACATTAAAACATTTCACAATTAAGCTGGTTC GGGGGFGGEGGGGGEGGGGGGGFGFGEGGFGFGGGGGBGGGGGGGGFGGEEFAGDGEEGGGFEGGGEGGEGFGGEEGFEEEEEEGEGGEEEBFDD?@68?; PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981 73 chrI 2900 60 100M = 2900 0 TCCCAATATAAACGATGTCAGTTCTGCGACATGCTACAGTCATCCACTAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACG CCCFFFFFHHGHHJIJJGIIJIJJJJJJJJJJJJJJJGIIIJJJJFHJJJGIIHIJJJJJJJJGHHHFCAEDDDDCCDDDDBDDDDDDDDCCDCCDDDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981 133 chrI 2900 0 * = 2900 0 GGCAAATATTATTTAAAATCAATCAAAATATAAAAAATAGTTCGTTCGCTGAAAATTTAAGCGTTTTCCGACCAAAATCAGCCCCTAGAGCGTCTATCCA CCCFFFFFHHHHHJJJJJJJJJJGIEHIJJIJJJIJJIJIHJIJIIJJJJJJHIHIJJJIJJIHIHHHHFECDBD?BDDDDDDDDDBDDDDB<@BDEDCC PG:Z:SNAP RG:Z:000 +EAGLE:2:60:6530:16775/1 0 chrI 2909 60 101M * 0 0 AAACGATGTCAATTCTGCGACATGCTACAGTCATCCACGAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACGCTCTAGGGGC GGGGFEGGGGGFGGGFFGGFGGGGGGGGEGEGGGGFGGGEGFFEDGGFGGGGFGGGCGGGEEFGEEGGGDBEGEGGEEEEEEBG?EGGBEFEEDDEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204 99 chrI 3991 60 100M = 4394 476 GTGCGCCGCGACTCCGCCCACCGGTGGTAACTTTTTGTGGGGGCATCTTTGTTTTTGGTCATTTTTCAGCGCTTTTCAGCGATTATTGACCAATTTTGAA ?=??;=@DDA1:?DBD:D::?@0;-;;-==7=C>>C8?A;>@8'3<5>AAAA????82?3>>3:>A>AA>3085<:>:>>5-28<>B>AAA<2>AAA>04 PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204 147 chrI 4394 60 27S73M = 3991 -476 TGAATTTTTTTAAAACATTTTTAGTAAAAAATTGTTTTCGTGCAAATTGTCTTCGATATTATCCAAACGTGACGTTTTGCGATTTTCGCGCTAAAATTAC ###########################C>@A?2?;?B@@C>(>C@7;;@@;>?C;@@C;=73@;8FF:=D80)8F:@B;819IHFBD<F8C=,;AA1B?@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:70:7573:20273/1 16 chrI 4573 60 101M * 0 0 TTTTCATAGTTTTTCTCTTTAAATAAATCACTTTTCAACAAAAAACTATGAGACAATAGTTTGAAATTACAGTATTCTTTAAAGGTGCACGCCTGCTCGAA CAA>C@EE:EEB?=DCBCC;DED=EDDCEAEECAA?GEAGGGGEEGBEEEGGEGGEGGEGGGEEGGEGGGFFFGGEFGDFGGFGEGGGEGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455 99 chrI 5186 60 100M = 5287 201 AACCAGCACCTCTGTTCTCGACCAACTATGATCATCATCGTCGAATAACCGTTTCTCGTGATTTGTCACATTATACTTGAGCACAATACATCCACCAGGT 5;5(@3.@############################################################################################ PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455 147 chrI 5287 60 100M = 5186 -201 TCAGTCCTTTCTGAAAATGAAAATTAATTTTAAAAAAATTGAATTATTTTAAATGAAACAGTTTTCAGAGATTTCTCAACTTTTGAGTCCCCCACCAGGC >8?A@BA:4(>AA>;5;:(:5A>>A@;>>;==6C==?=4HE@.@;FB@=4ECGGFB8=>B9*/910<@>B@>B?39DDCAGEHGC8?<C<)A?:?AD;?= PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:81:1948:19465/1 0 chrI 5499 60 101M * 0 0 TTGAAAATTAGGGAAATGACCGGACATAAGTTTAGAAACCCGATTTTTTCAATAAAAAGGAAAATTGAAAATTTAATAAAACAGGTTGTAAATCAAGGAGA FFFGGGGGGFGFEEFGGGGGGGFGGGGGGGADDD:EEEECGGGGFGGGFFEGGFGFEGGGEGFGGFGEGFFEGFDGGFGFE@DGGCFG?EBEBDB?EECEA PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:110:7845:14613/1 16 chrI 5562 60 57M1D44M * 0 0 ATTGAAAATTTAATAAAACAGGTTGTAAATCAAGGAGATCGTATTGATTGAAAAAAATCCGAATGTTCCGGATTTTTCAGTGGTTTTTTTTGAAAGAAAAT GFEEFFEFDGEGGFBGGFGFGEGGDBGFFGEGGEEGFFEBFDGGEEFDGEGGGGGGG:GGGEGGGDFEFEF=FGGGGGGDGGGGGGGFGDGGGGEGGEGFG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:110:4320:17819/1 0 chrI 7437 60 101M * 0 0 AATTTCAGTTCAGACCTCATTAAATTTGGGTATATTTCTAGGATCCGAGTTTTTACACCAGATTTACAAACTTTTAGCCTTTCACCGCCTTTTTATGCGCA GADGGGGFEEGGGGGGDGGGGEGFGGFGFGEGGGFFFFDFGFFEGFFFFDBFFF?BAFFFA5@CCFGADFGGGFBBBFEFGGEEGFFEFEBGGFECAFDD= PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1105:7234:86502 99 chrI 7663 60 100M = 7927 364 TGGCGTGCGTTGGCAAACAACAAGAAAGAATCATTCTCTGAAAACAAAAAGCGTTTTGGGTGCCAAAGTAATATTGAAAATCTGCCGTGTTTTCTCATTT @<@FFADDHDHHGIIIIIIIIIIIIIIGIIIGIIIIIIGIIIIIIIIIIIIIGEIEHFHF?DCEDCEC;@ADFEFDACDD@CCDDDDD@8<?AC@CDEEE PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1105:7234:86502 147 chrI 7927 60 100M = 7663 -364 CAGCTGGTATTATTTCAGACATGGTGCATCGAAAATTCGAAAATTACAGAAATTAACATTTTGGAGCATCTGCCAGAAAATTGAGATTACAGTACCCCAC CDDDDCDDECCCECCCFEFFFDFHGGHJIIJJJJJIHABHFJJJIIIIGFIJIHF@GIGIHGIGGGFHFJIJIDHIJJJJJIIIHIHF?HHHDDFFF@BB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:4421:72371 99 chrI 8558 60 100M = 8629 171 GCTCATCAGCCGTAATCAGGTGACCTCATTAGGCCTACCCAAACACAGATTTGTCATTATTTTTCAGACAAAAAACACGAAAAAAATCTTCACGCATGGG CCCFFFFFHHHHHJJJJJJIHIGHIJJGIJJIJJJJJIJJIJJJJJIIJJIJIJJJJJIJJJJJJIJJJJEHHHFCCDDDDDDDDDDCCDEEDDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:4421:72371 147 chrI 8629 60 100M = 8558 -171 AAAACACGAAAAAAATCTTCACGCATGGGGTGATAACCTGATTTTAAATCTTACTGTGCCGGCTGGCGCGGCGAGCTTCGATCACTGAGCCGAAAGATTA ?DB?DDBCDBDDDDCDDDDBBDCCCDDDCDEDDDDCDDDDEDDEDDDDCDDDCEEDDFHHJIIGIIJIJIJIIJIIJJIJJJIIJJJHHHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:79:1727:12868/1 16 chrI 11973 60 101M * 0 0 ACGGACCTCTGGGAGTCTGGAAATTCTTGTCTGAAATTATGCTTTTGAATGCTCGAAAGTGGTAAGAATTTAGAATTTATTACAGAAAAACGTTTAATTAA ABCC>CEEEEEEEEEEEEEEECCDEFFGEEEEEEGEEGGGGGGEEFGFEGGGGFGGEGEFGGFGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494 73 chrI 12099 60 39M1D61M = 12099 0 AGTATTGTATGCACTGTATCAAAACACATTTTCATCTTTCTAGGTATTCAACTTCACGTTTTTCTGTAATAAATTCTAAATTCTTACCACTTTCGAGCAT CCBFFFFDHHHHHJIIGIIEIIJJJJ?BFHJJIEIIIJJJJHJJ?DFGHIGGIJJJIIBHIIIIGIJIGHGGHIJJIIGHEIIEGHHHGGHFFEBCCDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494 133 chrI 12099 0 * = 12099 0 AAGTTTTTTTTTCCTTTTTTTTTTGGGACGAAAAAATTTGCAAAAAAATTTTCCCGTGGTGGAGAGTTGAACTCAGACAAAGCCTAAGCCTAGGCTTAAT CCCDDFFFHHHHGGHIJJFIJJIJBEH=@>AC@DDD<CDACCDDDDDBBCDDDDDBABBABD?ACBAC>CCCDDD@CC<?CCC8>CCCCCCCCCCC<CDA PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:6644:84389 73 chrI 12375 60 100M = 12375 0 GTACTTTTTAAAGGCACATTGTTTTTTTGGGTCAAGCAAAAATTTGTCGTGTCGAGACCGGCTACGGTATTTTGGCGAAAAATACCAAATTCTTGCGGAT #################################################################################################### PG:Z:SNAP RG:Z:000 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:6644:84389 133 chrI 12375 0 * = 12375 0 CCATATGATATCCAAATGAAAAAAATTTTGACTTTACCCCTTATCCTGTTCTACTCGGCCATATAGAGGAAGAAGTATAGCAAAGAAGAAAAACTGGACA :+=BD,=:C2A4C,++A+3+3++1:CGDB####################################################################### PG:Z:SNAP RG:Z:000 +EAGLE:1:37:10171:16610/1 0 chrI 12538 60 101M * 0 0 TTTGAAATCCAAATTTTTTGGATTGCGAAAAAAACCTGTGTCCGGTTGTTTCATTAGGCCAACAAAGTTCCTGGAACACTGATGAAAACCATGATAGAGGC GGGDGGFGGGGGAEGGGGGFFFFFBDFAFFEDFEFFFEFDFFFFFEEE?AAECCCFFFFFDFFFFDC@CCFEF:EFD=D?AEEEBEEDFEFBFEFBEDFE5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:14:9791:11230/1 16 chrI 12732 60 101M * 0 0 TATGGATATCATATGGATATCAAAATTTTTCTCATATGGATATGGAGAAAATTTTTCTCATATGGACTTTGAAAGTTGAATCACTTGACATCTGGGAAATT :CDDC=DD?FDGBGEEE=EEEEEEE@GGGBDGDGEFEGFGGGEFGGGGGFECDD?FGFFGGFFFF?FFFFFDDGGFGFGFDGEGGGGEGDGGGGGGEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:19351:44996 99 chrI 13367 60 100M = 13462 195 GCATAGAAATGATCATTTGGAAATCACGTTTAATTAGGTTACGGCGAAAATTTGCAAAAAAGAGCAGGAAACTTGGCTCAAATCCTTCGAAATATAACAA CCCFFFFFHHHGHJJJJJIJJJIHIJJJBGICHIIJIJGEHIEIIJJJJJEGIJJJHHHHFBDEEDDBDDDDDACCCDDDDDCCDDDD?B?BDDADECC@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:19351:44996 147 chrI 13462 60 100M = 13367 -195 AACAACTAGGACTTCCATGTAGGCGTTAAAGCGCCCTGTCTCTCACCCCAATCCGTACCTTAAGCTGAAACAAACGTGAACTTTTTTCATTTCTTAAAGG <>A>:CC@CCACDDDCCDDB?@DDDDCB@@BDDDCFFFDHHHEA;JJIGFHGJIHF?JJJJIIJJIJJHFJGHFJJJJIIJIJIIJJFFHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498 73 chrI 13826 60 100M = 13826 0 AAATAATTACATTCAAATTAATATTTTGTTTACGGCTATGGCCTAGAAATCGCGTGGTGGCCTAGGATTCATTTGCGCGCGAAATTCAAATTCAGCCACT CCCFFFFFHHHHHJJJIJJJIJJJJJJJJJJJJJJJJGJJJBHJJIJJJJJJJJJJJJJJJJHHHHHHFFFFFFEEDDDDDDDDDDCDEDDDDDDDCBDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498 133 chrI 13826 0 * = 13826 0 ATCTTAAAGAAGGAGTATCGTCAATGGGGAAGTTGTTTTAAAATGCAGTATTTGTACTCAAACTTTCAATTATTGCAAAAGAAAAAACGAAAAAAATCCG CCCFFFFFHHHHHJJHIJJJIJIIIJJJJHGIGHIHIIJJJJJGHIJJIJGGIJJJJJJJJIHIJIJJIJJJJIHFHHEEFFDDDBDDDDB@@BBBDD@9 PG:Z:SNAP RG:Z:000 +EAGLE:2:66:16575:12892/1 0 chrI 13844 60 101M * 0 0 CAATATTCTGTTAACGAATATGGCCTAGAAGTTGCGTGGTGGCCTAGGATTCATTTGAGCGCGAAATTCAAATTCAGCCACTTTCGTCGATTTCAACGGCA GFDGGEBGFGAEGBEGGGGGGGGGGGEGDGGFDGGGGFGBFFDFEGGDD?FGBDFGAGFGGGGEEGGGGGFBDGGCGEGEEEBGDGAB??BBBBB@ADADD PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831 73 chrI 13924 60 100M = 13924 0 CTTTCGTCGATTTCAACGGCTAAATGCGGAATGTTAACGGATTTTTTCCGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAA CCCFFFFFHHHHHIJJJJIIIJJJIJIJJGIGIIIIJIJJHJJIJJHHHHHFFFFEEEEEDDEDDDDEED=CDDCDEDDDA@CDDDDDDDDDDDDDEDDA PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831 133 chrI 13924 0 * = 13924 0 AAAGTATAGCACGGCCATGTGTCGATTTACGCAGTTCGTGTACTCCTCGAGGAAAAACTTGGATTTATTTTTAAAAAGGTTATTACAGCTTTAGGTGAAA @CCFDDFFHHHHHJJJJJJIIHHIGIJJIJGIIJFIJJIJGIJJJJJJJJJJJJHGHHFFFFFFEEEEEEEDDCDCCDDCDDDDEDCDDDDDDDDCCCCC PG:Z:SNAP RG:Z:000 +EAGLE:1:88:5129:6620/1 0 chrI 13927 60 101M * 0 0 TCGTCGATTTCAACGGCAAAATGCTTAATGTTAACGGATTTTTTACGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAAACAA FFFBDEAEEEGGFGFDFEFFGDGGGGDBGGGADEEEE:DEEECE:EEBEEBFFACDD@FBEFDEBBD=:EEAEEDDD@DBEEEEFDBBDDED?EBDEEGEE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:5:16715:13757/1 16 chrI 14162 60 101M * 0 0 ATGTATTTTAACACAGTTGTGACGTCATAAATGTATTTTAATACATTTTGCAACATTACTTGAATAACCCCATTAAAAATCAACTCAAGCATCAAAAAAAT :BB?EEEEEBEEEEAE=EEEEDEDEEBAEEEEEEEEEEEEEDBEBEEEE5EEEBE?E=EDEEEDEBADDDAEE?EDEEEEEEEEEEE=EEBEEEEEE?EED PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:28:17761:20206/1 16 chrI 14335 60 101M * 0 0 CAATTTTTTGTTTTTTGGTCCAACATTTTTTTTTGGTCTCAGCTCTGCTGCCTACCCTAGAAGAACTAATAGCGCTTCAAAAACTGATGAAAACGTTCAAA C:E?EEC?C9DDFGAEAEEFGEEEEGGCE?FBGGFF=GEFGCEDEE5DCAEFEEFGGGFGGGGGGGEGGGGGGGGGGGGGGGFEFGFGFFGGGGGGGFFFD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169 165 chrI 15544 0 * = 15544 0 GGCTTAGACATAGGCTCAGATTTAGGAATCTTCAAAAAATTGAATTCTAGATCTACATTATATTAAGCTGTCTAATTCTCTTCGAAAATTGTCGATTTTT CCCFFFFFHHHHHJJJIJJJJJIJJJJJJJJJJJJJJJIJJJIJJIJJJJIEIIJJJJJJJIIJJJIGGIJJJIGEHIHHHHHHFFFDEEEEDDBDDDDD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851 165 chrI 15544 0 * = 15544 0 TTTGTACTGTACCGCTACAGTAATCCGTTAGTATCCTAACATCTCTATACTTGAAGTTGAGATGAGTTGTATCTTCACAGACTACAAACTATGAACACAT @CCFFFFFHHHHHJJJJJJJHHGIJJJIJIJGIIJJJJJJJJJJJJIJJJIIJIJJHIJIJIIJJJHIJIIJJJJHHHHHHFCDFFD>BEEDDDEDDDD: PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169 89 chrI 15544 60 100M = 15544 0 AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGACCACTCACGGTGAAAAGGCAAAAATTCGGGGACCAACCAATAT ADBBBBDBDBBBDDDCADDCDDDBA?9DDEEEDCCDDBDDDDDDFHEIIEIJJJIHFHDBHGGHJJIJJJJJJJJJIJIIIHGIIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851 89 chrI 15544 60 100M = 15544 0 AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATAT DD@DDDDDDDDDDDDDDDDDDDDBCB?DDEEEEDDDDDDDDDDHHJJJJJJJJJJJJIHFIIIHIJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534 165 chrI 15545 0 * = 15545 0 GTTGAATGTTGATAGCTCTTCCGAGCGAGTTTTTTTAAATTAAGCTTCATAGCTGTAACATTTAATATGAAGAGTTACACTCAAAATAAAAAAACTGCGT ?@@DDFDDFHDHGGEEFFIHHIGGIIEGBDEHJJIICBFHIDEC;CGDEEDA@EGAEECH;CEEDDEFBC@CCA3>CCDD@CDCDDC>ACDDB####### PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534 89 chrI 15545 60 100M = 15545 0 AACTGCGTGGCGTGTACTGCAGAAAACCTCAAATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATCCGGGGACCAACCAATATC A8DDB@9-?@?:CCCCDCDDDCB@89?DDCAACCC?@DBDDDDFEDEHHBGEIJJIIHEFDIIIIIGIIJJJGFGGGJJHGFJIIJIHHHGHDFFDD@@B PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340 73 chrI 15561 60 98M1I1M = 15561 0 CTGCAGAAAAACTCATATTCAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACATCTC =B@FFFFFHH+0AEEEHHAHHHCGHEIDIJGIGIIGGEG=F?HGFHIGIB@AEEHHGFBFDDAC2;>C(9;8;?BB?<<A>@CDA>@4:<50&0>>@@>: PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340 133 chrI 15561 0 * = 15561 0 AGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACCTCTCACGGGTTCCGCGCGCCGCTATGTTTAACTCGCAGCGGGCGGAGACAGCTC A>AAAA@:B@>:BA8298<4(8@??:BEDB>43?5;00B@@;3+8(9?20&<:855'?@>DFIFIIIIFGFB?GFB)D0IFEIEFEEA?FDF?D;DD@@@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160 165 chrI 15566 0 * = 15566 0 TTCTCAACGGTGGTAGAATTTTCACAGAACCGTGCCATGATTCAGATTTTGCAACCCGCGCACAGGTCATGTTTGAACAAATGTTGTCAATCCCTATAAT CA@??829BCCC@::DDCDDEDCDCC@??BBDDDDEEEECDFDFECHHECHGE@HGGGGGGCCIGGF>IGGJIHGIJGHGHEEIIJIFHHHHFFDDF@CB PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160 89 chrI 15566 60 64M2D3M1I32M = 15566 0 GAAAACCTAATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTTGACCCAACCAATATCAGGCCGCCGACATCTCACAGGT DB@:3DEEDCCDDCD@DDDDB>??5?3C;;@>@CCBC>?BBADCCECEECCFFDEFEHHHHHHGEGIGGBGGIIIIIHGGJIIFJJHDGHGHFFDFFCB@ PG:Z:SNAP RG:Z:000 NM:i:8 +EAGLE:2:5:10071:18909/1 0 chrI 15576 60 101M * 0 0 TATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCC ECEEEFFFFFGGGGGGGGGGGGGGGGGGGFGGGGGEG?GGGFGGGGFGDBFFGGDGBGFGGGFG:EFDDFGGGGG=BF=FB@:D=BABACBEEGBBEC:E? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070 73 chrI 15598 60 61M1I1M37S = 15598 0 CGTCCACTCACGGAGAAAAGGCAAAAATTTTTACCCCAACCAATATCTGGCCACCGACATCTCACGGGCTCCGCGAGCCGCTGCGTTAAACTCGCTGTGG @@@FFFFFHHHDHIJJJIJCHGGF@FFGGHIJI<@D=FGGEHGGIIHI>FEFC-@;':B8CDE##################################### PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070 133 chrI 15598 0 * = 15598 0 AGTTTTTTTTTATTTTGAGTGTACTCCACGAGGATAATTTTTATAATTCTCCTCCTCTTTTCCTCGAGGAGTACACAAACTCCGTGAATCCACACATGAC ##DDDDDDDDDDDDDDCDDDCC@B@BDDDFFFFFFFHHHHHJJJIJIJJJJJJJJJIJJIJJJJJJJIHGIIHCHIJJIJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159 165 chrI 15648 0 * = 15648 0 GCGTGTACTGCAGAAAACCTAATATTTAGGCCCCGCCTTTTTATCGTCCACTCACGGGGAAAATGCAAAATTCGGGGACCAACCAATATCAGGCCGCCGA :?BD=0=AFHHGHIIJJJJIJIJJJJJIEHGJJJIIIIIJJJIJJJJJJIHGIIJIJHHFCDEEEDDDDDDDDDDDD@BDDDDDDDDDDEEECDBB@DD# PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159 89 chrI 15648 60 9S91M = 15648 0 AATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCCGCTATGATTAACTCGTTGTGGGCGCGGCAAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGC #########B>5BDCAC>:B?599>B@93BBBDDDDDDCDDDDDEDC@?DDDDDDDDDDBDDDEDFFFDDEEBIGIIJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:7173:80911 99 chrI 15656 60 96M4S = 15842 286 ATCCTACGGGTTCCGCGCGCCGCTATGTTTAACTCGCTGTGGGTGTGGCGAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGCGGCGCGCGGAACCCGTG B@CFFFFFHF@DFHIII1C6DDFHBHGHJHEHIHHCBEFAEFF9AACBBBDB@=A>A>@C<5@B-5>BB9095@>CCC@AC4@B-0559><B3@9@#### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:17151:68349 73 chrI 15710 60 68M1I24M7S = 15710 0 GTCTCCGCCCGCTGCGAGTTAAACATAGCGGCGCGCGGAACACGTAGGATGTCGGCGGCCTGATATTGGTTGGTCCCCGGATTTTTGCCTTTTTTCCGTG @@?DDFADGHHHDGHIEDCFEEH<DHGGGIBAGHEB??8;@2?:88(23(>@CBB8BB-77?9::>C:(+22;++:?A99-09CC@<@@C:>A####### PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:17151:68349 133 chrI 15710 0 * = 15710 0 ACACCGCTTGTTTCAGGGCTGTCGTTTTTTCGTTTATTCGGGTTTTTTTTTCGGTTTTTAGAACCAAAAAAACAACAAAAAAGTTTGGTCTGTGTGAAGT ######CDC>>C?<C??<3<99553B;BBBBCDDB?<;BB?;8?8;@AB>EHIGGGGGCHF=8A<7<D?BACGF?G<GHACC?;HGBHFFBA4BFDD<@@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:7173:80911 147 chrI 15842 60 100M = 15656 -286 GGTTTTCTGCAGTACACGCCACGCAGTTTTTTTATTTTGAGTGTATAGGTCTCGATTCTCGAAAGTATGACAGTTATTTAAATGATGAACTCGTGATGAC BDCDDDDDDCDDC>B;DDBBBDDDEDDFHJJJIJGJJJIJJJJIJJIJJJJJJJJIJJJJJJJJJJJJJIJJJJJJIJJJJJJJJJJHHHFHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:82:8204:15172/1 16 chrI 22312 60 101M * 0 0 GGGGGTACATGGACGAGAATTCTCTACCGTATTCCAATTTGGCTGACTGCGTGCTCAACGTTGAATACTCAGTGTAAACTTTCGTACACCGTTGCGTACTG E@EE@B@5BBB@@EE?BEB5BEDEEA?CEEE?DBEDEDEDEEE?EE?EEEEEEEEDEBEEBAEEEEBEEBEEEDDEDEDDE?EEEEDCDDDDAD=DC@CCC PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:88:6414:9094/1 0 chrI 23853 60 91M1I9M * 0 0 AAATCGAAAAAAATAATTGCAAAATCCGTCAGCTGAACATTCAAAACTTATCAATTTGAAATCAGCATATTTCAGTGTATAATTAAAAAAGGTTTCAAAAA GGFEFGEEDDDDEECFFFFFDFDDFFFFFFFEFFFFFFFEFFDFAAEEEEFFFBBDFFFFFF:FDCFF?FBFFEDEFEFFFFFFF?FFFFEA?BEFFFFEB PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:88:12428:19742/1 16 chrI 23928 60 1D15M1I85M * 0 0 GTATAATTAAAAAAGGTTTCAAAAATTCTGAGACCAATTTTTATTGAGAAAAATAATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGT GBD?CGDEEEEFGFEFFD?FFFDFFDDEEAEE=EEEACCFE?GBFFDGDCGFFFBEDGFDAGGA=GFGFBGFFFGGEG?GDGA?DGEDEGEEEFFDEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:39:4472:4563/1 16 chrI 23983 60 101M * 0 0 ATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATT 6=CCDDGEEEEEEEGEEFGEEDEE?DEEGDGDBGEEGGGEGDGGGEFFCFEAGGFGGGGEGFGGFGFGGGGGFFDGDFDEGFFFDEGFGGGGGFGEFGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:48:8251:3932/1 16 chrI 23995 60 101M * 0 0 AATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCGTGCTACAAGCCTGAATCTTTCAAATTAAAAACCAGCATGATTTTTTCAATATTC @DDBD????@==>@>8::FEEEGE@EFGEEGEECACBBCEGEGFGDFFFFBGFEBGFGGGGGFGGGGDBEGEEE5EGEGGGGGGGFGGGFGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:96:5841:4603/1 16 chrI 24002 60 101M * 0 0 GAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATTTTTTCAATGTTCTAGGACG EDBE=EBE?BBEFAED<<<CA??DC???A;ABCBCE5FFEACEC=EEDE=DFGG=GDFFDGEDEDEDDDD5DDDD?:?FFGGFGEFFDFF?FFBEFEFF?E PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:51:3584:15408/1 0 chrI 24181 60 101M * 0 0 AAAAAAATAGTCAAGTTGGGCCCATGCTGTACACCTAAATCATTAAAATTCAGAACCGCCATGTATTTTTTCTTACCAAAGGCTCTTTAAAAAAAATCTGG =EDEED>AAAEA?BB:DDDADDED?DDDD=DDADDEDEE?EBDEEEEEDDEE5C:EBEE?E=EBBEBE>CEEEAE=?DEDEEBEBDEEE=?BEEACCEEEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539 73 chrII 202 60 100M = 202 0 TAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGCCCGTTCACCAAAAATAGTGACTCTGACCGTTCACTA @CCDFFFFHHHGHJJIIIIJJJJJJJIJJFAGGIJJJJJJIIJJJJJJJGIJJJICHIIEIIJJJJGHHFFFFFAA??ACC;ADDDDDCCDCBDDDCDDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539 133 chrII 202 0 * = 202 0 CACTAAATTTGGTGAACGGTCATAGTCACTAAATTTAGTGAACGGTCAGAGTCACTATTTTTGGTGAACGGGCAGAGTCACTAAATTTGGTGAACGGCCA CCCFFFFFHHGHHHIJIGIFGHHIIHIIJJIJGIJJJJFHIJJJIHIIIHFFGHJIJJJJJJIJHIGIIHHGFDDDDACDDDCDDDEDDDDDDDDBDBB> PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938 165 chrII 231 0 * = 231 0 CTAAAAATAGTGACTATGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCG CCCFFFFFHHFHHJJJIJJJJIHJJJJJJJJJIJJJBBCFHEHDFBHEHGHHIJIACFAHGIFFHI@GDGAEABBDFECEEDD??BDDCDCACCAC5:A< PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938 89 chrII 231 60 100M = 231 0 TGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACTAAAAATAGTGACTATGACCGTTCACCAAAT CA>DB?BDDBBCCC@<DDCEDEDDDDCC>BAB?BBABCDDB?HHIGGHGHGGHC;GJGGHHIJJGHECAJJJJJIIJJJIHJJJJHFHHHHHFDFFFCCC PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480 73 chrII 234 60 100M = 234 0 CTCTGCCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTTACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA CCCFFFFFHHHHHJJJJJJJGIJHIJJJJJJJJJJIJJJJJJJJIIJJJHIJIGJIJIJJJGEEHHDFFFFDEBCEDDCCDCDDDDD@DDDDDDD@CDC@ PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480 133 chrII 234 0 * = 234 0 CGGCCAGAGTCACTAAATTTGGTGAACGGTCATAGTCACTAAATTTGGTGAACGGCCAGAGTCACTATTTTTGGTAAACGGCCAGAGTCACTATTTTTGG @BCFFFFFHHHHHJJJIJJJIJ@FHIJJJEGIGIIGHIJJJCHJJJJIIIFHIJJJJJJJJDHHHHFHGFFFFCDECEDDDBBBDBA:ACDC>A@CDC<9 PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038 73 chrII 234 60 100M = 234 0 CTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA CCCFFFFFHFHHFJIIIJIEHIGEEHGIIIJIJJJJFJIIIBGHIIJIJDDIGJJJJJJJJIIHHFGHHBDDEEEBDEEEDCA@C?@BDDDDDDAACDCC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038 133 chrII 234 0 * = 234 0 GTCAGAGTCACTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG BBBFFDFDFFHHHJJJJIJJHIJJJGIIJJIIIFGGHIJJJJIJJJDGIIHJIIGIIHHHHHDDDFDDEECC=ACCCBB8;BDDDACDDCCDDDACACBB PG:Z:SNAP RG:Z:000 +EAGLE:1:59:19240:17867/1 16 chrII 234 60 101M * 0 0 CTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACCAAAAATAGTGACTATGACCGTTCACCAAATTTAG =?CCCC:5@?:CA56;7???:EEDFAGEGEGGECFEAFDF=GFGBFGGGEGBGGGGEGGGGG=GGGFEGGGGGGGGGGGGGCCCCC7DDDDFGGGGGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459 73 chrII 238 60 100M = 238 0 GACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGA @CCFFFFFHHHHHJIIJIIAHHIIJJ@HHGIGHIJJJJGIGIJDHIJIJIGGIJJJIIIJEIJIEHIGHHGHFDEEFFACCEDDDCDDDD@DDCD@>@CC PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459 133 chrII 238 0 * = 238 0 GTCAGAGTCACTATATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG ?@BDBABAFFHHHJJJJJJJEDHIJJGIJJHGICGHIJJJJGIIJJIGIJJIJJJJGIJHHEEHEE@@CFEF=AC>CDDDDD??B:@ACAACDAAAAC?? PG:Z:SNAP RG:Z:000 +EAGLE:1:50:4361:1823/1 0 chrII 240 60 101M * 0 0 CCGTTCACCAAAAGTAGTGACTCTGACCGTTCACCAAAAAGAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC GGEGGGGGGGGGGGGGGGGGDAFFFDFFFFGGCGFEGEGGFC@ABFEEFEGEGEFCFGAEEEDE:DD@>CC5@ACAC:CCC:C==?A?@=:;;;=@CA?@5 PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:85:17281:13087/1 16 chrII 240 60 101M * 0 0 CCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTC ==<76>CF@CAGEDEFEEC?EED?CDBCB:=EDEGGGGCDEFFEE?FFFDFDGGFFFGEFFGFEDFFGGDGGGGEGGGGEGDDGGGGGGFDGEGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:78:10927:5210/1 16 chrII 240 60 101M * 0 0 CCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC F@?@:B;EEEE:D?EEDEEAFEFEEEFFDCEGEDAEFAFDFEDFFDFFFFGGFGGBGGGDGGGGGGGGGGGEGFFGGGGGGGGGGBGDGGFGGGGDGGEGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:20571:27060 165 chrII 241 0 * = 241 0 AATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTCAGTGACTCTGGCCGTTCACCAAATTTAGTTACTCTGGTCGTTC @@BFFFFDHHDHGIJJJIIJJJJJJJJJJGHGIHGIJJJIIIJAHIJJJJJIJJJJIGGHIDAHIAHIIHFDDFFEEEBCCDCDDDCDCCCDCDCC==<A PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:20571:27060 89 chrII 241 60 100M = 241 0 CGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGATCGTTCACCAAATTTAGTGACTC 8CDDBBDDCD@5:DCA@@;;CCDBAADED@?EHCEFGHJJIGHEFJJIGHIJIFDIHGIHJIIEGIIIHGFJJJIGIGIHEHGGJIJHHHHHFFFFF@@C PG:Z:SNAP RG:Z:000 NM:i:8 +EAGLE:2:77:9514:17665/1 16 chrII 241 60 101M * 0 0 CGTTCACCAAAATTAGTGACTCTGGCCGTTCACCAAATGTAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCT EBB@B?DDDDDBBE=EBB5??B?EEDEEBBEBBEBACC?ACDDD@DEEEDEEDEDCEDEEEEEEEEEEEEBDADDDDDDDBDEE:EDFDFFEEDDECEDBE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:97:16219:20975/1 0 chrII 244 60 101M * 0 0 TCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGC CC=CC?DBDB=@@?D:?AA?CD:BBABA?DBE?BEEB>???@>>>@?:EBB?:B:B@EEBBEA?B@?@>8@@>@@;?<??@@?<?8???<=?7?@=<<>>? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:55:11493:15098/1 0 chrII 245 60 101M * 0 0 CACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCC 5FFFFFFFD?ADBDDFFFAE=?DDBFEFFFAD?F;DDDADEEEEEFABC=B?EEEFDBFE??DAA:?@=B?C=@B>5=@@;8;87>>=>>B?5BAB6@::9 PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:61:8991:6659/1 0 chrII 246 60 101M * 0 0 ACCAAATATAGTGACTCTGACCGTTCACCAAGTATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCG FGFGGGGAGGEDGGGGGGEGGGGGFFAFFFFF:FAD=@CDEEE=EFFED=AAAABFBG:BBBEAEEAEFE=?EEE=>=>>GDDEDDB@D@=A@@?A:BBB9 PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:73:10783:17479/1 0 chrII 249 60 101M * 0 0 AAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCCGTTC GEGGEGDGGGAFFFFGDGGFEGGGGGGGGGFFGFEFFGGGFFGGGDBGGGGGGEFDGBGEBEBFDFCD?ECECDCBDFDEEED?EC<BCCAC?CCACAA=C PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:47:10664:4304/1 0 chrII 249 60 101M * 0 0 AAAAATAGTGACTCTGGCCATTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTC FGGGGGFGBGDGGGGGGGGFGGGGGGGEGGGGGGEGGGGGGGDGGGEGGFGEGGGGGGDGEGDEGGFGEGGGCEEGEGEEEEEEEEEEDEEEEEEEEE?EC PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:84:13989:21133/1 0 chrII 251 60 101M * 0 0 AAATAGTGACTCTGGCCATTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCAC GGGAGGGGBGBFFFFGGGGGGGGGGFGFFGFGEGDGFGGEGGGGEEGEGGFGFEGEDEEEDGEEEEEEGEEEEAEEEEEEBA?A@CACACEB?EEA?AC?C PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:46:5068:2631/1 16 chrII 254 60 101M * 0 0 AAGTGACTCTGACCGTTCACCAAAATTAGTGACTCTGGCCGTTCACCAAAATTAGTGACTCTGGCCGTTTACCAAATAAAGTGACTCTGACCGTTCACCAA CCCBC?C?C?@:CC=?::BDCDFD?EDFF=EFFFFDGEGGEG?GDGDFFF?B?FFFEDGDGGGFGGEEDBBFEEFFFGGGGDFFFFGFFGGFFAFFDCEEE PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:14:17135:3972/1 16 chrII 259 60 101M * 0 0 ACTCTGGCCGTTCACCAAATTCAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGATCGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAAAATT 5A?BA=@CCBA5ECEEABCC=DFFBE?GFGFEFFGGDFGEDGGGG?FGEGBGDGGGFGGDGGGGGGGFGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:50:3266:18098/1 16 chrII 264 60 101M * 0 0 GGCCGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATTGTGAC B;????BC@C?B:@?B@EB@E?EBEEE@DEEEEC:E78A@5D?CECE@EBECD;?DBEBD?EEEEEFBEFFAFEFDFFFEEEEAAE?EEE=BEEEADDDDB PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:51:8846:13213/1 0 chrII 675 60 101M * 0 0 TTACCGCCACCGATGTGCCCGACGCGACTTACTGTTAGCCCTTGTTTTGCACAAATCTGTTGGCTTCCATATTTAAAAGTTAATTAATGACCCAATGTTCT A<6?;@>@BBEEEEEEEEEEEEAEEE?E?AEEEEEBDEA?C@>@=EB<8BEEBDDBDBC???CC5@B??@?EBEBEDEE@A:@?B:?=@=@:B@B@???BB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:6280:52543 99 chrII 1371 60 100M = 1443 172 GCTCAGGACACCTCATACAACTCCAGAGAAAATGTGTCTCATTATTCTTGTCTTTTTTCAAGATCTAATCAATTTTCTACATTAACGACGTTTTTGTCGT CCCFFDDDHHHGFGHGGJIJGGGGGIHIGHIJIIJI?FHJIJIJJJIIEIGEGGEHIIJAFC@CHHG>EHH@CEFHHHCDE@C@3;A59;8<CBB@B@0< PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:6280:52543 147 chrII 1443 60 100M = 1371 -172 TTTTCTACATTAACGACGTTTTTGTCGTTCTGCTTCTTTTTTTCGTTCGTTTGTCTCGTCCATCAGCTGTCCACTCATTTCTCTCCCACTCACTAGGCAG 9CAC@@:CA?DB@@<?BDBDCBB@B>DDC?>:>9DBBBEFEDFEGIIFJJIHA:IJJJIIGF>GIIGIGIHFC@FFGFJIHDGH?GHFHHFFFDDFFCC@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113 99 chrII 2989 60 100M = 3262 369 AGTATGGACAATTGTGAACGGAGAGGTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAA CC@FFEFFGHHGHJEIGIJJJFHAHICGFGIIGIGHGGGHHFHIIJJIJ4@EGEDAEECHHFFFEBDFEEEEEDDDDDDDC@BCCDEDDDDC?CCCCDA@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213 99 chrII 3014 60 100M = 3127 213 GTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAACAAGAAACCTGTTGGAGCGTATTTC CCCFFFFFHHHHHJJJJJJJJIJJEHIJBGGHHIIIIJJEHIIFIJIJIIJJJJJJJJHHFHHHHFFFFFEEEEEECDDDDDDDDDDDDDDDDD9BDDED PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213 147 chrII 3127 60 100M = 3014 -213 CGAAAATATCATTTGCAAAAAAACTTGAAAATTGAGAAACTTTTTTAAAAGTGGAGTAGCGTCTGCGGGTTTTTTTGCCCTAAATGACAGAATACATACC BEEEEEDCDDCDDDCDDDBDDA>DDDEDDEDDEDDEEDDDDFFFFFFHFHHFJJIJJJJJJJJJJJJJJJJJIJJIFJJIIIJJJJJHHHHHFFFFD@CB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113 147 chrII 3262 60 4S96M = 2989 -369 TGCGATTTTTATTTTTATTTTTCATGAATGTTAGGGGCAAAAAACCCACATGCGCTACTCCGCCTTAAGAAGAATCAGCTGTGAGCACTATCCACTATAC ####?CCC@C?:@DCDBBCC@>CCDDCC@A<<?DC>A;?BBB;5EEECIHBA@9ED?@0FDBGFIEHIIJIGJHGJJIJJJJIJIHHDHGHHFFFFF@BB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:87:12598:13225/1 16 chrII 4380 60 101M * 0 0 AAATTCATGAAAACTTTACTGAAAAACGAAGACCCATTCGATCATGCAAACAAAATATAAGTTTCTATGAAATGGACGGGGATATAGAAGAATTTGAAGTG DAAA?CCDACC?=?CAB<A@ACCC=C=@B@?:CCEEE5FGFFFGGGGGEGDGGGGFGGGGGGGGGEGFGGGFFGGGGGGGGGGGGGGGGGGGGFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:82:12769:6412/1 16 chrII 5968 60 101M * 0 0 TAAAATATCAGTACACTCCTGGTAGAACAAATATTTTATTTCAATGTTTATGTATGAGTAGAAAAGTTAAACAGAACTATTATTTGGCATCAATTTCTGTT GEGEGEEDFEGGEGEGEFGGGGGFGEEGGBGFGGEEGFGGFGFGFGGGGGFGGGFGGGGGGFGGGGGGGGGGGGGGGFGGGF?GGGGGGBGGGGDGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2303:1316:84598 99 chrII 7139 60 100M = 7167 128 CATCTACCGTATATTCTTTACTAGTGCTGCAGGCAGCACTAATTTCTAGGCCCTTTTTTAATGCAGTACTATTAGAGACTGCAGTACTACTGGAGATGCA @C@FFFFFHFFHHJIGIJJIJIJI4BFIEHCGHFGGDHGIGGGIGGIIBGGDHIDHIIIGIIDHIIJFEHHHHECC@DFBEDDD>CCEACD@C?<CCC3: PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2303:1316:84598 147 chrII 7167 60 100M = 7139 -128 GCAGGCAGCACTAATTTCTAGGCCCTTTTTTAATGCAGTACTATTAGAGACTGCAGTACTACTGGAGATGCAGCGCTAATAGAGAATATACGGTATGTAG DCC<CA>3CDCDCACC@;EC?=<FEGIGGGGIGIGIHG@JGGIHFCIHIGHGIHGDFIHEDHGJJIJIJJJIIIEEJIIJFAHDGEIFFDHFFFDDB@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:25:17975:18268/1 0 chrII 7306 60 101M * 0 0 CTTAGAAAGGCTAATATTATTTTTAAAATCAAGATTTAAGTTAATTAACACGACCTCGTATTCTATATTTCAGATATGTCAGTTATGCATGTGTCATGTTT GGGEFGGDGGGGGFEGGGFBFGGGGGGGEGFGGGFFGFDEEGGGE=FFFFEEAAECE?CCEEGEEE@DADEEEGB?:CACFEACECEFBCDBEBECDEC:? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:15150:63415 99 chrII 7998 60 100M = 8021 123 GTCTATGGAACAAGAAGGAAGACGAGAAAGCGTGTAAGACCATGAAATGTTTTTTGTGTGCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGT C@@FFFFFFHHHGGGGGIJJIJIJJIJJJIIIIJIJIIGIJJJJJIJJJJFGHIJJHHEEFFDACBDBBB@CCDB=@BC:@D@@CDDDEDC>ACDDDDBD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:15150:63415 147 chrII 8021 60 100M = 7998 -123 GAGAAAGCGTGTAAGACCATGAAATGTTTTTTGTGTGCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAG CCCBBDD@DDDEDCDCDDEEEDACBDDDDFFFHHHGIIGIHDIIJJIGIIGHJJHIIIJJJJJJIIJIGHFJIHEIJIJJJJJJJIHBHHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834 99 chrII 8057 60 100M = 8275 318 GCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAGATATTGATATTTTATATTCTGTATGTAGGCGCTGTG 7BBFFFDFHDFDHEHGGIAHGHIJDHIIJJJJBGFHI;DHBGIJJJGHIJIIJIIJJJJII)=EHFHHFFFFFFFFEFDEEEEDEDDCDCDCAA?@BDBC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834 147 chrII 8275 60 100M = 8057 -318 GTTCGTATTCTCTCAACTCATGGCACTACTCTCGATGTGCATTGCCTTTTATGGTTGCTACGTTATGGTTCCTCTTGGAAGAGAAAAGCATGCTCCATAC BDDDDDDDDDDDDDDCCEEEEECCFEB;FHHHHGDGJIJJIJHGIIJJJJIHCJIHGHHFJIJJIJIJIIFIIIJJJJJJIIHJIJJHHFHGFFFFF@BB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:79:2735:13063/1 0 chrII 9267 60 101M * 0 0 TACACTATTCTTAACTTTTTGACATGAATTAAATCTTTTTAAACTGAGTCTAAGCGTATTATTGTATCGTATTTTCCCCTTCTGATGTATTCATTTACTTG ADEBED=ADC=C6=CD=DD6C5BA>A?DDDEBE=AACCA<@B:EEEAEB?ED=EEA<AABCCA@:BB?B@5ACCCEBDEDEBBE:5??:?=@:?@E?BCBC PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:17065:72325 99 chrII 9394 60 100M = 9559 265 CAACTACCCAATTTTCCTGTGCCATGTTTCTTGAAACAAATCAAATGTGATAAATAGTTTGAATGCCTTTATGTATAATAAAATCAATTTTTCAAGCCGG CCCFFFFFHHFHHJJJJBIFGHJJJJIJJJIJJJJIJJJJJJJGGIIFIIHIJJJJJGIIIJIJJJJJJJIJJIJIJIJJJJFIHHHHHHFDFFBECCC> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:17065:72325 147 chrII 9559 60 100M = 9394 -265 ATGAAAAGCCTGTTCTTTGGTGTTTAAAGGTAGCACACAAACCAAAATTTGTTAGCGCAAATTTAAATTTCTATTTCAGTTACCATGAGTTAGTCTAACA @DDDDDB?@DDDBDDDDDDDFFFFFFHHHHHJJIHGGJJJHJJJJJIGIHJIIJJJJJJJJJJIJJJJJIEIJIJIJIJJHFJIIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:91:5280:21105/1 16 chrII 10935 60 101M * 0 0 CAATGCCAATACAAAATAAGTCCGAACAGCCAGGTTAACAACTTGAGTATAAACCAGAGGAATCGGCACCATATCGAAAATGACCAGATTTAGAATTTTTG 8==?=AAA??589??A8A5A?BAA?>D?ADAABABADAEE?EBDDBEBEGEBBFFD=:?;5ABEBEGEFFEGEBDGFEFFGGBGGEGGFFEGGFFFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871 99 chrII 11660 60 100M = 11873 313 TTGTTAAGCAATATTCTATAAATCACTGATAAAATAGAATAACACAAAAGCCAAATTAGTAACTCTGACCATATTGATTTCCAAACCGATCCTTTCCATC CC@FFFFFGHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIHHHJIJJJJHGIIIJIIIIJJJJJIHGIJJJGGHHH=BFDDDDDDDDDD@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871 147 chrII 11873 60 100M = 11660 -313 TTGTATCAGTCGGTGGGTGAGCGATTAGTATCAAGTAGCACAAACGCTTCCGTTTCAGATTTGCATATTTTATTGTTATAGGGGTTATTCAGGCATAGGT DDDDDDDDDDDDDDDDDDCEDFFFFFFHHHHHHIIIJJJJJJIJIIHG@JJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:118:13551:9082/1 16 chrII 13278 60 101M * 0 0 TGATACTTATTCCAATTTCATTCCAATTACATTCATGCTTGGATTTTATGTCTCTGCTGTTTTCACACGATGGTGGCAGATTTTCGACAACATAGGGTGGA EBCDADACBC:EFDEBDFEBEDEBEF=EBFEEBFDEEFEF?FFDFFAAFEFEDGFDGFGGFEEE?EEGEGGGEGGGGGGFGGGBBGFGDGEGGGGGGFGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335 99 chrII 13632 60 100M = 13690 158 TTTAGTTACTGCTGGCTTGATGACAGAAAAAGAAATGGCCGAGTTCGAGTCTATCCCTAGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTT CCCFFFFFHHHHHJJJJJJJJIIJJJIJJJJGGIJGIJIJJGIFHIJJJGHIJJIJJIJJHIJHHHHFFDDDDCDDDDDDDDBDBCCDCCDDCDCDD9BA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335 147 chrII 13690 60 100M = 13632 -158 AGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTTTCGATGATCACTTTAGCGCGAGACGAAGGAATGATTTCAAGTGATATCATATATGTAG CCCDDBB?DDDEEFDDCDDDDDEEEEEFFFFFHHHHJJJJJJJJJJJIIGHFJIJJJJJJJJJJIJJJJJJJIJJJJJJJJJJIJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:47:2491:18712/1 16 chrII 14238 60 101M * 0 0 GAAAAATTTGATTATTTAATTCAATTTTAAGTTAGAAAAATATCCTACACATTTTCTGAAGAAGTGTCGTAAATGGGGAAACTTTTTAAACATGTACGTTC EEEBCEAGEGEGBFEFFEDEDDEFGDEGDGGGGEGEGDFFCEFFFFDFDFFEBEEFBFGFGGGGGGGGGGGGGGGGGGDGEGEGGGEEGGGGDGFAEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:57:14985:11088/1 0 chrII 15505 60 101M * 0 0 ACTTTAACTCTCCTGTCAAATACAACACTGATGGGATGAAAGACCGAGAGCTTCAAAATCCAACACCAATCACTGATCACATTGATTTGCCTTTGCATGTG FFFFFFADFFDAA?DEEBEEDFFFFFDFFFFFFFF?EEEEFFFFFF=F?BFBEDDBBCBBFEFEDFD?=E@@E@B:=??CDD:EDDBB5>>=5=>B??B?C PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:21:7517:15303/1 16 chrII 15804 60 101M * 0 0 TTCAGGTAGTGATCTAGGCAAGTCTGGAAAGCGGGAGAGAAAGAAGAGCGAGTGATTTTTGAACAGTATGATAAAATATTTTTTGTTTCTCTTTTCACTCT E5AFEBAFDEDEFFCEAEC?ED?DAEFFCF?FFFFFFBFFFFDDFEDDDD?FADFFF=DFFFFFFEEF=FFFFFFFFFEFFC>FFFAEGFEFDBDFBDDEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901 99 chrII 16035 60 100M = 16399 464 GTTCTATAGGACCCCCCCTAATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTAT CCCFFFFFHHHHHJJJJJJJJJJJJJIJJHIJJHIJIIJJJJJJJJJHHHHHHDBDDDDDDDDCDDDDDDDDDDDCDCCDBDDDDEECCDDCBDDDD?C@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578 99 chrII 16055 60 100M = 16433 478 ATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGT 1=BD=2=ADCFHHGJJJJJJJJJJJJJJJIJIJJJIJJJIIIJJJIJIJJHIJJJHHGHHFFFFFDEEDEDCBBDD@CCDDDEDEEEEDDDDDDECCCDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:74:13479:19610/1 16 chrII 16067 60 101M * 0 0 GAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAAC EEEFBEECBDDEEEEEEEEFEEEEEEEEEGEGGGFEEGFEFBEFGFGEFGGGGGGGGGGGEECEE?GGGGEGGFEGCCDADD:DDDGGGGGFGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:60:16490:10079/1 0 chrII 16083 60 101M * 0 0 TTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAACTGAGGTCGCGCTGGAA GGGGGGGGGGGEGGGGGGGGFGGGGGGGGBGGFGFFGGGFGGGGGGGGGGGFDFGGFGFGGGG8GGGEEGGC?FGDGDGGGEGGGEGEGGBDEGEBAECCG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885 99 chrII 16208 60 100M = 16423 315 CTTCTATAGGACCCCCCGTTTTTTTTCACGATTTTTACTAAAATCAACAGATTTTGGAATTTTTGACAAAGCTCAAATCAAGTTTGAGTTAGAAATGAGT CCCFFFFFHHHHHJJJJIIJIJJJJJHJJJIJJJJJIJIHHHHHHFFFFFCEEEEEBDDDDDDCB@DCCCDDDDDDDDDDDDDDEDDDDEDDDDCDD>CD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901 147 chrII 16399 60 100M = 16035 -464 AGTCTGTTTCAAGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACA ABDDDDDCBCDCDDDDDDEDCFFFGGHGIJJIIEIGGCJHDB@IIGIHEGIGIIIIJIJIIGGHCIHCIHJJJJJJIJJIGIGGEIHHFFCHFFEFDB@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:79:2445:14024/1 16 chrII 16410 60 101M * 0 0 AGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCAT CACAACF=CCDEG@GEFDEAEE:EEFG5EEGEFEFEGFEGEGEFFEGGGGGGF=EGGEEGEDGGFFEFFBFFFFFFEEEAEGGBGDEGGGGGFAGGGFFEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885 147 chrII 16423 60 100M = 16208 -315 TCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACC >DDDCDDDDDDDDDEDEEEEEEECB?B>FHHEHGHGIJJIHGJJIHIGJJIIIIJJJIHHDIIIHHGJIGEJJIIJJIJJJIJJJJJHHHHHFFFFDBCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578 147 chrII 16433 60 100M = 16055 -478 GCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAAC CDDDCDC@:@DCDAA>DCEDCDED@CBCEHCBHHE@EBIJHFAGHGHEEC;ECGFFDJIJIJIIIEIGGHHEIGIHGEJJJJJJJIHFHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:84:7059:2112/1 0 chrII 16465 60 101M * 0 0 CCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCA GGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFGGGGGEGCGGGFGGGGFDGEGGEGGEEGGGEEEEEEDFCD@EEADDEEEEBECE>C@BCC<E=EBEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:23:16149:11703/1 0 chrII 16484 60 101M * 0 0 CACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATA EEEEE:E?D??DDCADBEEEBEBDBBDBED:DDD5;A?A5C?CC@BEEEEBACBB?5BCEBE=BEEEBC=:@:8<:?5B?E=BB?:BBBBADAA5BE?B=D PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:90:13478:14626/1 16 chrII 16489 60 101M * 0 0 ACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAA :CDD@CEAEEEDC@BCFDEBC=FBFDEGEEEEEGFFEGEGFGEGGGGGGGGGGEGGGFGGGGGGEGFGGGGGGGGGGGGGGGEGGGFGGGFGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:111:13020:19687/1 16 chrII 16513 60 101M * 0 0 CCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAAATCAAGAAAGGATCAATAGTTAAT EEC?=ECCBGEBEAFEBEEGGEGEEF=EE5EGEEGGEGGE?EGEGGC=GGEFEFAGGGGGGEGGDDFGGFFBBFGGGGFGGGGGGDGFGFGFFFFFEE=EE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:6052:32630 99 chrII 16537 60 100M = 16752 315 TATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAAATCAAGAAAGGATCAATAGTTAATCGGGTTTCCTTGTGTGCGGATGA @BCFFFFFHHHHHJJJJJJJJJJJJJIJJJJJJJJJJJJJJIJJIJJJJJJIIJJJJJJIJJJJIJIJJJJJGIIIJHHHFBDDCEEEEDDDDCDDDBDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:21126:70246 99 chrII 16655 60 76M24S = 16691 101 CCATTGATCTGACCAAACTTTTCAGCTGGTTGTCCGGAATAGACTTCCAAGCGTCGAGAATTCCTTGCTTCAACGATGCAACTGTTGGGTAAGTCTTGTT +=?DDF;?CCFFF<EEF@FI>FDCFEHIIBDEI99:C06DB<4?BBBB<@?67B=AAHG/AAC:?DFDDF(66>>@######################## PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:21126:70246 147 chrII 16691 60 35S65M = 16655 -101 CATTGATCTGACCAAACTTTTCAGCTGGTTGTCCGGAATAGACTTCCAAGCGTCGAGAATTCCTTGCTTCAACGATGCAACTGTTGGGTAAGTCTTGTTC ###################################?C=)?7).):;@CAA@<D?DDBB90)6?BDC9C8;EE:<EAD@FF::?EC3ED:<+:D:D:;?=? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:6052:32630 147 chrII 16752 60 100M = 16537 -315 GTTCTGAGCATACACGATACGGACAAGAATCCCCCACAAATTTTCGATTGGATTGAGATCAGGACTTCGAGCTGGCCAATCAAGAAGGTTGATCTTCTTG DCDDCDEDEDDDDDDBDDDDDDDDDDDDDDDFHGGDJIJJJIIJJJJIJJJJJJJJJJJJJJJIJJJJJJJJIJJJJJJJJJJJJIIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2309:5944:13872 99 chrII 16838 60 100M = 16910 172 GGTTGATCTTCTTGAGCTTGAAATAGTCGCGGGTTGAGTTGCTCACATGGATTGTCGCATTATCCTGCTGAAATCTAAAGTTTTTTCTGGAGTAGTGACG BCBDDDEFHHHHHJIJJJJJJJJIIIIGGIJJJDGGGGCGGGIIIJJJJJHHHHHGFFFDDDDEDEDDDDCDDDDCCDDDDDEDDDCDDCCDACDDDEDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1312:16439:9749 99 chrII 16889 60 100M = 17198 409 TTGTCGCATTATCCTGCTGAAATCTAAAGTCTTTTCTGGAGTAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTT CC@DFFFFHHHHHJJJJIJGJGIIIJJJJDCHIJJJJJJGIHGGHHJJIJHIJJJJIIIIJJIJJIJJJHHHGHHHFFFFDDEDDEEDDCDEDDDDEDC@ PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2309:5944:13872 147 chrII 16910 60 100M = 16838 -172 ATCTAAAGTTTTTTCTGGAGTAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTTCATCTTGCTACTGACGAACTG CCDDDDDBBDDDDDDEEEEEEFFFFHHHHHHJJJJJJJJJJJJJIJJJJHJJJJJIHJJJJIJJJJJJJJJJJIJJIJIJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:10135:61245 99 chrII 16930 60 100M = 17321 491 TAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTTCATCTTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCT @@@FDFDFFFHHDBFHIBFG@@BGEGHGIGHEHICHGIGIJEGHGIIJGGFHIDEGHFEHIJHI;F>EHIJCEHBHFFBC3;ACCECCCCC@CDACACD> PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:75:15288:18925/1 16 chrII 16992 60 101M * 0 0 CTTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTG CC@CDD?DDDAEEEEEEDDBEFDEAEEGBEGEEGEEFDGGEGFGEGFGFFFFFFFAEEEEECGFGGEGGGGDFGGFGFGGGGGGBGDGGGGFGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:48:9783:12656/1 16 chrII 16993 60 101M * 0 0 TTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTGG =???=7A???A5==>:A??=A5BA?@BBB?B:BAAA5?A>:BA=BBBABBD?B?BACBCDBDCDCBDDBCDBDDDDD?DDBDD?DC>>B==DD=ADDD:DD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1312:16439:9749 147 chrII 17198 60 100M = 16889 -409 CACTCTTGCTTTTTTAAATTCTCACTTTACTCCAATTCGTTCCCATATTGTTCTTAGCAAATTCCAATCGCTTGAGTTTATGGTCTGCAGAGAGTAACGG 4@DDDDDDDDCDDEDCEEDCCD@;@CDDEHHFHFIIIJIGIJIJJJJJIJIHIJJIJJIJJJIJJIIGJIJJJJJJJJIIJJJJIJJHHHHHFFFFDCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:10135:61245 147 chrII 17321 60 100M = 16930 -491 CGATTACACCAGATCGTTTGATGACATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAGCTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGA 0:@@@AA:CCB@DB@DDCCDDEDCCCEEFFFFDHHHHEBIJIHJIIGIGEGAGEJJIIJJIHGCHBGHGIGIIIJIIIIJIGJIJGGFHHHHFFFDF@B@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999 99 chrII 17346 60 99M1S = 17605 341 ATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAACTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCG @?@DDDDABFHCFEFHIIII=HHCFGHGABBE3<DGHG>@FFH<FFDF=H>=CBCFEGGAAGIGHHEE>5@@B@C@CA?B=?AB;;?B<:@:A>3+8??# PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:3353:52872 99 chrII 17407 60 99M1S = 17466 159 TTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCGTCACGCACGGAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGAT CCCFFFFFHHHHHIJJIJJJJJJJJJJIJIJJJJJJJJJJJJJJJGIJJJHHHFFFEEDEED@BDDDBBDDDDDDCCDDDDDDDDDDDDBDDDDDDDBD# PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:10693:67001 99 chrII 17455 60 100M = 17585 230 GAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTAC @@@DDDDFFHHGHJJJGIGJIGGIEGHIJIGGGDDBGHIDHIIGIIGCAEEHFFFFBCCECCCCDDDDDDDDDDCAADEBBBDDDDBDD<BCCDDDDCDA PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:3353:52872 147 chrII 17466 60 100M = 17407 -159 GCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGATCCTTCAGATACACGCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGC >DDBDDDDCCCCDCCA:CDDDDBBCDDEEDEEDFDHHHIIGGIJJJIHGFGIGJJJJJJIGJJJJJJJJIJJJJJJJJJJIJJJJIHHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946 99 chrII 17486 60 96M4S = 17520 134 ATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTT <@@FFDDDCCFGHIIIIGFH6EFGGCH>@H<D9B?F:FA4@FCH)).746=??B98/3953(;;>(5>:>:>@A>;5;>(:48&8@?BC>3>C>CC#### PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946 147 chrII 17520 60 100M = 17486 -134 GCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAA ><CD>C@4:<BB>@C@<?D?A>CC;@9C;CB?DCDCEEAHEHHA@CCC88;GHCIIGGG<DHBHFBFDCGFGDJGJIHGBJJJIF@<8DAHHFFDDD@@? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1105:2590:61441 99 chrII 17529 60 100M = 17793 364 GTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGAT CBCFFFFFHHHGHJIJJJJIJJJJIJJJIIIIIIJJJJJJJJJJJJJJJJHIJJJJJJJIJJJJJHHHFHFFFFFDDDBBDDDFDEEDDBDDDDDBBDB3 PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:108:5608:15871/1 16 chrII 17573 60 101M * 0 0 CATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCA ;5D???C=FDEEEAEB=?@??CC::@:CBBC?BBEBD5CCC:GAGFDGEGG?FGDEGAEGBGFGGEEEGGGGFDFGGGDFFGDGEGEGGGEGGEFFGBGDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:10693:67001 147 chrII 17585 60 100M = 17455 -230 TCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCAGCCTCTATATG :>A>@4C@AC>CCAB>5B=3,(@>@@CDCFFGHHECIHGECCIDGF@:DEG@BFGHGDGAGGDFD4FBCEGHGB@EGGHGGEFGIGIHHHHHDAFFFC@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999 147 chrII 17605 60 18S82M = 17346 -341 ATAACATCCAGCTGAGCGCGTTCAGTGCCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCAGCCTCTATATGTG ##################?CCC?A;9''<?>ECA?7=DC=)';7''**8/9<GD?09?*G@?9C9:*)FE?++HBBHC3@CGAHHEFA3:DFDDDBD?:; PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:56:7956:12354/1 16 chrII 17709 60 101M * 0 0 CCACATTTAATAATTCACGCAAAAAATAGTAAATAACATCTGTGAGGGACAATTTAACTTGAAATATTGGTCCCATGGAACCTTGTAATCAAAGAAAAACG AEBEEEBBDEBDEBBFBFB=CEEFEEEEGGEDEDAABAB:AEEEEEDFADFFFBEDEGCEGFDFCFFAGDEGFGFGGGGDGDDFGFFDEFGGGGGGGEEGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1202:3232:33845 99 chrII 17750 60 100M = 17828 178 GTGAGGGACAATTTAACTTGAAATATTGGTCCCATGGAACCTTGTAATCAAAGAAAAACGATTTGATTCCTGATAAGCCTTCCATTGTTTCCTGCTGCAT @B@FFFFFGGHHHJIJJJJIJJJGHGIIJHHIJIJJJJGJJJJIJJIIJJHGIJJJJIJJJJJJJJJJJIHHHHHHFCFFFFEEDEDDEDDDDDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1105:2590:61441 147 chrII 17793 60 100M = 17529 -364 GTAATCAAAGAAAAACGATTTGATTCCTGATAAGCCTTCCATTGTTTCCTGCTGCATATTTTGCCAAATCAGCTTGACTACACAGTCGAAACATCTAAAG CCCDDDDDDCDDBAADEEFFFFFHHHHHHIIGFJJIIIGJIGGIIJJJJJJJJJJJJIJIJIJIGIJJIJJJJIIJDIIHIIJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1202:3232:33845 147 chrII 17828 60 100M = 17750 -178 CTTCCATTGTTTCCTGCTGCATATTTTGCCAAATCAGCTTGACTACACAGTCGAAACATCTAAAGTGCGTGCTAGGAATTTTAGTAGGAACTTGTTAAAG DDDDDDDCDDDDDEEEEEFFFFFFEHHHHHJJJJJJJJIIJIJIGIHJJIJJJJJIJJJJJJJIJJJJJJJIJJJJJJJJJJJJJJJHHFHHFFFFFCCB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:7586:92994 99 chrII 18220 60 100M = 18302 182 AGATAAACTTCTGGAAAAAAATGTATATAGGTTTTTTTCAATTTAAAAACGAACGGCATTAGCAGTTTTTCGAAAAAATTTTCGGAAGTTCCTCAAACCT CCCFFFFFHHHHHJJJJJJJJIJIJIIJJIIGHIIIJJJJJJJJJJJJJJGFIHGFFCDDDDDDCCCDCDDDDDDDD@DDDDDDD<@BCDEDDDDDDDBA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:7586:92994 147 chrII 18302 60 100M = 18220 -182 CGGAAGTTCCTCAAACCTTCAAATTAGGGGGGGTCCTATAGAACTTTCCCACACTGTATATTGCAAATACATGACATAAATTTAGATGCAGGGCAAAAAC DDDCDDDCCDCDDCCDDDEDEEDDDDDFIJJJJJIJJIJJIGDJIGIIHEFIFIJJIJJJJJJJJIIHCJJJJJJJJJJJJJIIGIIHHHHHFFFFFCBC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:17571:72038 99 chrII 19694 60 100M = 19888 294 CCTCCATTTCCTACAGAACCTGCGGTTCAAACAATTAAACTTCCCAAATATATGGAGGTAACCATACACTTTTCATATATGTGTAACATGGGGCGGAAAT C@@FFFFAHFDFFIIIIGHGGIIIJIGIIG<FGHIJGIJJIIJJIJIJGHIIIIIJJIJGIJGHGIGGEEFHHHDBDEEDDDCCCDCEDD?@B>@BDB9@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:17571:72038 147 chrII 19888 60 100M = 19694 -294 CAAATTGTTATTTATTTCCGATTCATCTTTATAACTTCAATAATTTATTTGAGAATTCTATTCAATGCTATAAAAGTCAAAAATTTGCGAAACGTGGTTT ADCCDCCDDECC>AACDBFEHEFGHCD:EGGDHHFA>@IHGIJJJGGGGGGHEHFAEHIIGGJIIFF@GGD@IIJIGGIIIGHGIJJHHHFDFDFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394 99 chrII 21802 60 100M = 21978 276 CTTCTGAGAAACTCGAACTCATGATCAGAAACGCGCTCTGATCGAACATTCATACTCTAACACATTCCTCTTCACATCTCCAGATGATTATTCATGTCCA CCCFFFFFHGHHHJIJJJJIJJJJJGGGIIIGGIJIJJJJJJGIGHIJJDIGIJJJJJGDEEHHHDEFDFFEECEDCEACCDCDDCCDCCCDDD;3;A;5 PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:89:11611:13275/1 0 chrII 21896 60 101M * 0 0 TGTCCATCATTTTATCATTAAATATCTCATTCTATACTCTCTTCTCGCTTTATTGTTTCTCGCTCACTCCCTACCCACCATTACATAACATCTCTGAAATT EEEEEDEEDEEEEBEFFFBFEFFEEFFFEEGGGGGDGGEGGGDGGGGGFGGGGGGFGEGGGFEGGFGGGGGEEGGGEGE?ABBEEEEGEEEEEEEBEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394 147 chrII 21978 60 100M = 21802 -276 ACATAACATCTCTGAAATTTCAAAGTTTTGACATTCTTGGCTGTGCCTTTTCCTCTCAATGATATTTTCCAAATTCCATTATTTTTCCCCCGCCTTTGAT 3CDDDEDCCA>3@DDDDDCCACDDDCCCDCDDDDDDDDDDDDBA??CCA<ABDBDDDEEEEEDCDCDCDDDCDCCCCCEEFFFFFHIHGHHGFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:109:12018:8736/1 0 chrII 22087 60 101M * 0 0 TGGTTACTTGTTTATTGGTATACTTCTCGGTATTCTTTTTAATGTTCTGCATGTTTCATATGGTATAAATTGCATATTACGTTAGACACAAATTACTGCAA :CC=ADDDDCFFFFFFFFFEFFFFFFFFFFDFFFFDFFEFF:FFFFDDFFEBEEFBBDEDEEB=DFFD5DBDEEEFFFAEEF?F:D:EBCFBBFFFDFFDF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:83:14451:5491/1 16 chrII 24168 60 101M * 0 0 GAGATGCTGGAAGACCAGGACCAGTTGGAGAGCAAGGACCACAAGGACCAGAGGTATATTTATTTTTATTCGATATATTCAAGGCTTTACATTTATTAATT FBBBAB=@DAEAB5EEEEFBF=EDEEFFEEEFFEBFBBCBBFFEFE@CEEE=FFFFFEFFFFEEFFEFEBFEFFFFFFFDFBFFFFDEC:EFDFFFBFFDF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:12843:33593 73 chrII 24481 60 56M44S = 24481 0 GTACAGAGAGTGTAGATAGTTAGAGAGGGACATACATCCGGGACCCAGTGGGGCGGGGGGGGGGGGGGGGGGGCGGGGGGCGGGGGTGGAGGAGGGGGGG @?@DFFDEDFADCGEGBEBAEHHCCEA+@@;?CFDD<?F:FB6@FH<D=F@=B8>@############################################ PG:Z:SNAP RG:Z:000 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:12843:33593 133 chrII 24481 0 * = 24481 0 TCGAACTTTCTCGTGCAAAATCTCTGGCCTTTTCTGTATACTTTAGAATTTGAATTTTCATACGCATGGTTTTCTACACCAAATGAACACATCTGAGAAA @DEDCDDDBBDDEEDFFFFFFHHHHJIIGG>JHIGGIIHGJJJJJJJJIIJJJJJJJIJIGCJIJJJJJIIIIHGFGCJJJJJIHEGHFHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670 99 chrIII 1739 60 100M = 1876 237 CACCACAATTCAATACTTTTCAACTCTTGAGAATCGAAATAGGGCAGCGTAGATATAATATCATTGGGTGACACAAAAATAATCGATAATTCCTTCACAT CCCFFFFFHHHHGJJJJJJJJJGJJJJJJJJJJJJJIJJIJIIIJIJJJHFHIJJJJJJJJIIJJJJIEEHHHFFFFDDECEC@BDBDDDFACCDCCDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670 147 chrIII 1876 60 100M = 1739 -237 TGTGGTAACGTTAAGGTCTTGTTGAGTTTGTACAACAAATTCGAATTTCCTGACACGTCCCAGTAATATTCTCAAATCGTTGAAAGCTAGTTGCATGAAG BDCCBDCCDDCDDDDDDEDDEFFDDFHHHHHHHJIJIIJIIIIJJJJIJJJIIIGJJIIJJJJJJJJJJJIHJIJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:51:14937:4579/1 16 chrIII 4052 60 101M * 0 0 CTCAAAAAAAATTCCAACGAACAAAATGTGTAGAACTTTACAAACTTTTTAATAAAGATTTCCAAATTTTTATACAGATTCACTAAATTTCCAGAGTTTTC EB7<<?BBAB=E?BEDBFC8DADBEDBEBBECD?@A@AC=CE=?ECDFFFAEEBEED?B5BB:EBEAFFAEE?BDECAC=CBADDEDEA?GGFGGGF:EFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:9937:9794/1 0 chrIII 7949 60 64M1D37M * 0 0 CATACAAATTCGAAGCGGTATTGTACGATCAAGCTGTTATTCACATTTTTTCATCCCAATTTTTCCAATTTTTGATGGAATTTTTGCTCAGCGAGCCTAAT GGGEGEGGGFGGDDGGGGCGGGGBGGFBDGGGFGGBDDEFEEFEEFEFFGFEEEEGEEDEEEGFEEEEEGEEFE:CEEBCEEDEEBDDBCCDC?CEEEEE5 PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:120:8762:5690/1 16 chrIII 9639 60 101M * 0 0 CGCCGCGTAAACTTTGAAGCTTTTTATCTTTCTAGGAAGAAGAGTTATGAAAAAGTTGTCATCTAAGAAATTGTGTAGAATTTTCAGCTCTATAATATAAT 5D=AB=FFEDBFFDEFBEDCCCFEBFDDEEFFFFEFBF?FFFFFFEDAFDBDE?EBDFAEEFEFFFBDDBAFAFFFDDFFEED?EFBEEEA=EBEDDB=AD PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:44:18899:7734/1 16 chrIII 11923 60 101M * 0 0 AACGAGTTTGAGTTGTAATAAATTATTAATTTTTTAAACTTTTTTTCCCAACATTTTTCAATTTTTTTGGGTCCCAGGGGGAAAATTGATGGGTATGCTGT DAEE@EE@C@?GEEEGEAEEEEC=ECABC9EDDGDDCAA?CCCC::EAFECF:GGGGGGGCCFFF=B=AA?F?AFFGGGGFGGGEGGFDGGGGGFDGEGGD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:3105:84335 99 chrIII 15487 60 100M = 15624 237 GGCTACACGTTTCTACATGTGATCATTCTATAAAGAATGGAGTGGTTGAAACTTTTGATTTTTTTGCTAAAAATTTGAATTTTTTTAATTGATTTTTTGT CCCFFFFFHHHHHJJJJJJHHIIJIJJJJIJJJJJJJJJIJIGFHCGIGIGHIJJJJJJJJJJJJHHHHFFFFFFEEEEEEDEDDDDDDEDCDCDDDB<A PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:3105:84335 147 chrIII 15624 60 100M = 15487 -237 TTTTATGCTAAAACTAAAATTTTCACGCCGAAACTCAAAAATTTCACATTTTCACCTTTTTTCTTGCCATTTCAACACTTTTTCCTGTAATTCTCTATGC DDCCEDDDDDEDDCFDCA<DDB@DDBDFFEHEEHHJJJJJIJJJJIJJJIJIHDJJJIJJJJJJJIJIHIIJIIGECJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:114:2367:17023/1 0 chrIII 15792 60 101M * 0 0 TTTTTTTCCAACAGTTTCAGATTCATGATTCCGTCTACTTCAACAGATTTGCCAATTCCTGATCATATTACTGTGAGTTACAAAAAAATTTTGGAAAAAAT GDGEDEGGGGGGDGGGGGGGFFAFFFFFFFFFFFFFFFFFFFFFF?FFF?EEEEDEFEFDEFEFFFBFFFFEDDE:FEFDFFFAFAC>CCFFBF=FFABEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103 99 chrIII 19688 60 100M = 19760 172 CTGTAGAGACCCAGGCAGGCGAAACGGGTTTGGCAGAAGCCGCTTCGTTACGCGACAGAACCATCCCCCACCCGCTTGTAAATATATACGCAATCAAGAT @CCFFFFFFHHHHJJJJJJDGHIJIIIJ@GIGHHHICGC;FABEGEEHFFDDDDBDDDCCCDDDDABDDBBBDBB@@DBCDDDDEEEE@BBBBDDDDDAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103 147 chrIII 19760 60 100M = 19688 -172 CGCTTGTAAATATATACGCAATCAAGATTACGCAACGCAGCTGAACGAAAAAAAAAAAGAGAATGGGGGAATCTTTGAGGGGAGATAATTGAGAGGAAGG @DDDC@CEEDDCCDDBBCCDCCDCAADBBDBDBBBDDCDCADDDDCEDHIJJJJJJIJJIIIIHJJJJJJJJJJJJJIJJJJJJJJJHHHHHFFFFF@@? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934 99 chrIII 21761 60 100M = 22497 836 CAAGGATCTATGCATGATTTGTGGTAGATGTGCCTGGAAAAGGGGGATTTTTGAGTTGTTTTCTAGGTGTGACCAGGTTTTTGATATGATTTTTGAGAAG CC@FDDFFFHGGGGGGGIJIHFHIIE>B4<<?FGHIIDFIGEHGIJAEHBCC@AE@GHEHHFEEDFF);7;>AAC>@AC@BB?<C>>@>CDCCD>8<<>< PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934 147 chrIII 22497 60 100M = 21761 -836 TCAACAAAAAAAAATCGAATTTGAAAACTTATAAAATTTATCCAATAAATGGGCGGAGCTTAGCCAGCTTTCTCAACAACTCACTTGCAAGGTAATAATC CACB<@<B:CDDC@@B;DFD>;?EEA>EA@74JJIFCC;GHF:GIGIFBDGG@EGB<HEIHF?ECBHHB9E>DCA@>CAEHHGIJJJHHFFGFFFFD@<@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:41:8435:11353/1 0 chrIII 24117 60 101M * 0 0 AAATTTTGGTGAAAGTGGGCGGAGCCTAAGTAAAGGATATATTTTTTAAAAATTATCAAAAAATGTTGCAAACTTTAAAAAAAATTTTAAGTTTTTTTTCA GADGGFFFEEEEEEEEGGGGGGEGGGGFEG?BBCEFFFFFDGGFGEGGGCFGGFGGFGGEEEDEGEGGGFEEDEGGEEEGED@=6DD?EBGADBDDB=C?E PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:95:11679:7360/1 0 chrIV 621 60 101M * 0 0 ATTTCCCCTCAATTTTCTTGCAGATCGACGTGGATATTGACGACCTAGATGACGATTTTCTCTCCACAAACGGAATGGAACGACACGGTTCAGAATTACAA EF@@@EEEEEBBABBBECEEEEEBEBEFBDFDEAFEGD5DGGDEFGGGGDFGFG:DFFFFFGBGGGGGFGF?F:FEADBEEBEEEEBEBEEEE==:EAAEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:58:15136:13881/1 16 chrIV 723 60 101M * 0 0 GAGAAGTGGTTTTCACTACGACGAGTACTGCGAATAACAATGTGAAACGAGCCGTCGAATATTTCGAAGATGATAATCAAGATGATGCACTAACATCCACT @A9A9AAA??A?:?:??;@?:>>??=5A?A?@:C=CCADBCDDCFBAGEEEEFFGEFDEFFDG?GGFGGGFGDDGD?FFDGAEE=?EECDAFEEE?EEEFE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:25:10330:11791/1 0 chrIV 1025 60 101M * 0 0 TTTATTGAGGAATACTCTATTGAGTTGGACACCTCTGGGAAGTATGTGTTGGGAAAAAAAATCCGAAAATTGATTGAAAAATTCGAAAAAAAATCCAAAAA GGGGGGF=GGEGFFGGGFGGGGEGEFBFDFGGGDGGGFGFEFBFFEBEBEFFFEFFAFGGGEFGGEE=GDEDEBEGEDEDEFEEF@DEEEEG>CBBDDEBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:29:6386:17433/1 16 chrIV 1252 60 101M * 0 0 AATTGATGGATAAAAATTCCAAAAAAGTTCCAGAAACAACATTTCCTCCGCAGCTTCACCTGGTCCCAAGTCTCCATTTGACGATGATTTTACTGATACAG E?===FBDGGFDEE=FE@GGFEEBFACAAD=FFFDEFEDGGFFEDFBAFGGFDFFF=BGGFFFDF=GDAGGG=GGEGFDGGGGEDGFFDFDEEEEDEEEDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229 99 chrIV 1474 60 100M = 1594 220 GTCCGGTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTA BBBFFFDDHHHHHJJJJJJJJBHIJJJJIGIIIFHIJIJJIJJIJIIJJJJIJJJJJJIJJDHHHHHHHFFFFFEACDDDDDDDDDDDDDEDDEEDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819 99 chrIV 1479 60 100M = 2024 627 GTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTAGATTT <@@DFFFDHHFHHIIIIIIIHI>FAFHIHGHEDHGCFHIG?F?DFHIIFIGHHGGG.=FEGFH>CDHGBHH2?@C??>>;AC@5@B3;5;>::>>>(:@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229 147 chrIV 1594 60 100M = 1474 -220 CCCAACCCTAATATCCACAATTTTTTTTCAGTCTGGAAACTCTAACGAAAACCAGCTCCGCGAGAAGAAGCTCCACAAAAAAGAGCAACTAGCTCACCGG BDBBCDCCDCCCDCBC@DDDDDDDDDDDDDDDDEDDCA;DDDDDBDFFB?=HHHIJIJJJIJJJJJJJJJJJJHFJJJJIJJJJJJHFHHHHFFDDFCCB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819 147 chrIV 2024 60 18S82M = 1479 -627 ATTAGAAGAGAAGAGGAGGAAGAAGTGGTGGAAGAAGATCCAGCAGTGGTAGTTCCAGTTCTACGGAAGGATCTGGAAGTTGAGGAGGCTCCGAAATCGG ##################CAA>;A;(>D?;>?HEA=74E:C=>@@;=8=89FBFDCGFD;69D:?AD?9?1*9<:9E??AA+;FGFGD<:8A;BA=+1=: PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211 99 chrIV 2692 60 100M = 2824 232 TTTCAGACGTCTGTATCCGCCGATCCAAAATCTCACACGCCGATTTTGATGGCCGACGAGTACAAAGAACCACAGAAGCAAGGTGAGTTTAGTTCGAAAA CCCFFFFFHGHHHJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFFDDDDDD8ACDDDDDDDDBDDDDDDDDACB:CCD>@CEDDDDDBDDB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211 147 chrIV 2824 60 100M = 2692 -232 CATTGTTAGAAAATTTGAAAAAAAAACCAAAAACCGTCGTAAAACTACACTTTTTCTATTGAAAATAGAACAAACAAATCAAATATCCTACATGTAAAAT :@4(DDDDDDDDDDCDDDDDDDDECDFFHHHHBJJJJJJJJJIGIIGHGJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562 99 chrIV 2921 60 100M = 3122 301 AATTTAAATCGAGTGGAATTAAATTCTTCAAACAAAAAGAAATCTGCATTTAATATCGATTTCTCATTCAATTGTTATGAATCATAAAACATGATTTTTT CCCFFFFFHHHHHEHIJHIJJJJJJIJJJEHJJJJIIJJIJJJJJJJJJIJJIJJJJJGIJJJJJIJJJJJIIIHDHHHHHHHFFFFFFEDEEEEFEDD@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562 147 chrIV 3122 60 100M = 2921 -301 ACAATTGGGATTAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAATGCTTCTTCAAAACGAAAAAATTGCACACTATAAAC CDDCCCCCDEDDDDDDCCECACEEEFFFFFFFGHHHDJJJIIIIJIHIHFHJIJIIJJJJIJJJJIJJJJIHIJJJIJJJJJJJJJIHHDHHFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:51:5632:6609/1 16 chrIV 3456 60 101M * 0 0 GATACTGCATTTTTCCGGAAAAGGACAAAGTCGGCTAATTGCCGTTTTTTATAATTTTTGCTCATTAAGCTTATTCCATTAAAAATACAATTCTTGTTTAA ACA;C@CDBCCEEEEEEEEEEEEEEEEEBEEGEEFGEBGEEGGEEEBEEDGEGFEFGGGGGFEGGGGFGGGGGGGGGGFGGEGGGGEGBEDGGEFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:11958:20308/1 0 chrIV 3987 60 83M1I2M1I8M2I4M * 0 0 CTTTGAAAAATAGGGAATTAAATTTAAATAAAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAAATGAAATATTCAGGAAAA GGGGEGGGGG?CCFFGGGGFEGGGGGFDGGGFEGGGGGFGGGGDGFFGGGGEGEE?FDFCGEEEEGGEEEEGEEEEEEE@CDDCCEBDDBEAEEEDBEEEA PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063 99 chrIV 4720 60 100M = 4891 271 TTCAAGAGCCTAAGTTTTTTTTTTCGAAATGAGTTTTTTAAAATTGATCGAAATGTTTTTAAATACTGAATATTTGGGATTCTAGATTTTTACTACAATA CCCFFFFFGHHHFIHJJJIJJJJJIJJJJIIJIFHIJJJHHHHHHFFFFFDEDDDFEEDDDDCDEDDEDDDEEEEDDDDDDDDEDDDEDDDDDDEDDDD> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063 147 chrIV 4891 60 100M = 4720 -271 TCTAAAAAATTTTTTTAGTGCCTGCACCAGTTGTCGTTGCTCAGGAGTCGCCGATCTTAAAACGAAGAAACTCTCTAGTACCATCAAGGATCAGTGGAAG CCDDCDDDDDBDDCDDDDDDDDDDDDDDDDDDDFFFFHHHHHJJJJJIHFJIGIJJIIJJIHIJJJJIGHJHHDJIJIHHJJIIJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:49:18520:10880/1 0 chrIV 6174 60 101M * 0 0 GATTTTGGATTAAAAAATTCCGAATAAAAATCAACTTTAATTTAACGAAATTAATTTTCTATACCTAATTCTCTAATTTTTGCAGAACAGACGTAACGATG EEEBEEDAA?DDB?ADEEEEEEEEEEEBEE>EEEDE?EB=EEEDBEE=EEEEDE5?ADBDEEEEEEEAEEEAEBE:DDBCDEE?EBDEDEEEEB:EEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:16672:84478 73 chrIV 6412 60 56M44S = 6412 0 TTTCGATATTCAACACTACCTGCCCAAGAAGTATAGCTCATTTGAAGATCAGTCACGGCTGAAGAACATTCTCGCCGAGCTGCACGGGCATTACGCGGGC 1==A1AA+<4,2>A3A3A7A@=?AABC?9AA:=<*7?*=*:7*0***9?79<A?AB############################################ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:16672:84478 133 chrIV 6412 0 * = 6412 0 AAACGGAAATGCTCCGGAACCTGATGTTGGCCTCGTTGCGGACACACGATCCACATGCGCTCCCAAATCTCGGTGTCGCTGACAGATTTGAATGCATTTG +:8;?2=:CDB4B4+@#################################################################################### PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:20092:23229 99 chrIV 7114 60 80M20S = 7551 528 GCCGTATCCTCTTTATTTGAAGTGCATGTAAATTTAAAAGAAGATACTTTTCGCTTTTTCGAGTGACCTTTTTTCAGAGAAAATATGTGCTTTTCTTGAA =??DDDBD?DDDD?,C?BFDC@,<EAFE+AEF<FFD9B4?CDDEAF<9*:9B?0?8?D>=ADA8677)7A7AED7;3??@#################### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:20092:23229 147 chrIV 7551 60 9S91M = 7114 -528 CAGTACTCCTGTATTAATCCTACAGTAATCCTAGAGTGCCCCTACAGTACCCCAATAGTATTTCTACTGTACTCATTTACTCCTACACCTAGAGTACCCT #########C>>3@>>;)@@BDCA=)>?77@@CGEG@;F;94?/AD@?0BBDHHFHFB9BBB@FDF<CCHAE?GCHA?AC+HEBDGAHHBF??D?AD@?B PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:25:14196:18951/1 16 chrIV 8235 60 101M * 0 0 TTGTTTTAATTAATAAAATACGTACACTAATTCAAAATATATGTTTTATCGATTTTAAAACCTCAAAAAAATTGTAAAAAAATTGAAAATTAACTGAAAAT CEEEEDAFD=CEEEBEBEEBEGFFGFEDGGGAGEEGGDGEGFGFEDGEDDEGFEEGGGGFGGGGGGGFGGFFFFCFFDFDFEEEEEE:FFBFFF:FEGGF? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:115:8880:13347/1 0 chrIV 8542 60 101M * 0 0 GAAAGCGGTAAGAATTCGAAATTTCAGTCAATATCCCTTTTCCTCGGGGTGTACACTAGAGTTGTGTAAATCTACACATAACGTTTTTTCGATTATCTCTG GDGGFGEDBGFFDFFEGGGGEFGGGGGEEG:EEBEGGF?DEGFBDEAAE<A?CCBEEDEBE?AB=:CCCCCF?EBFD:DFBDDCCCBCACE@EEECEA:55 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:25:8054:8779/1 16 chrIV 8985 60 101M * 0 0 GGATAGATTTCCATGTGGGCCAGGCATTTTGCTAGCTGAAATTTTGGGATTTTTACTTACAACTGGTTTAGAATAATTTAGAAATTACAAAATCGAGGTTT 5B5EBEBB?C=E5:=EEFEFDEBAEE?:D=ECE::DFFAFFFD:=FEBDDE?B:ADBBBAECEBDBDBCFBEEDDADED=?ED5?DE=:DEFDDDD?EEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:7767:73428 99 chrIV 9395 60 100M = 9538 243 ATTGTAGGCCTTGATAAGATATATGATGACCCGTCGTTGAAACCACGTGGCTGGAGATTAATTCTATCAGTTAGTCAAAATTTACTGACAATCACTCAGA CCCFFFFFHGHHHIIJJJIJIIJJJJIJIJJJJIIIJIIIJHIJJIGIGGIGFHGHIJJJJIJHHHHHHHFFFFEEEEECE@CC@@CCCDDDCDDCCDD: PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:20580:46115 99 chrIV 9443 60 100M = 9624 281 GGCTGGAAATTAATTCTATCAGTTAGTCAAAATTTACTGACAATCACTCAGACGCTCAATATTGCGATTTTGCTTCAAAACTACTGTACCCGGTCTTGAC @@;DDA+B<FDHFC?EHGGGCIHICF3AFHFCFFCGEGGCFHIHDDD>FHGIHGIII6B8=B8@=<;=DHHECCEACCB@@@:>CCCCC@CBBBBBC<5@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:7767:73428 147 chrIV 9538 60 100M = 9395 -243 TTGACATTTTTTTTGGGAAACATGAAAAAAATGAGCATGATTTTTTTTCTATTTTCGATAGACCTGAATATATTTCCGAAAAATTCAACCCATCCATCGC EEDCCDDDDDDDDDDCCEEEDEDDBDDEFEDDDDDDDEEDDHJJJIIJJJIGFIJJIIGGHFJJIJJJIIGIIJJJJIIJJJJJJIHFHHHHFFFDDCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:20580:46115 147 chrIV 9624 60 100M = 9443 -281 AACCCATCCATCGCTCACTTACTGTGAAAAAGTTCCACTGCAGCCCGAGATAGTGATGTTTTGTGGGCGAATAGTTGTCGAGAAGCGTTCGCATGTTATG 89<2AC?5BBB>A>@C@DDD@@:CCCCC@A@>A;>>;;?1DCBB?=;CC=4C7.)ADF@BDB;0>F?F?B@HDAHBD?CEHGGGFC@87HHD?ADFD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:45:8531:9371/1 16 chrIV 10605 60 101M * 0 0 TTTAAATTCAAAAATTTACATAATTTTTTAGTTTCTAGTGCCCTACGTTATTTCTCCTTCCGATAGAAATAACACTAAATCGACAAAACATTTTGCTCAGA :CAEFEDCEGFEDDEEDEEDEGEFEG?FEFDEF?FDCGEGFFGEFGGGBGEG?GGGGFDGGGFFGGEGGGEGFFGGGGEFGGGFGFGGGGGDGFGGGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:45:5297:17573/1 16 chrIV 10799 60 101M * 0 0 AGAAACAACACAAAAACCGGTAATCATTATGGAAGTTCGTTGTGTCAAATTTGCACGATTAGTGAAAACGCTGATCTTCGATGCTTAAAGACTTCATCGGA EBBECBBDC@EDFDCCFBAEEEEBGFGGGGEEFFGFEAFDBGEFGGAGGGEGFGDGFGGBGDBGGEEGGGFGGGDDFBGFFEGFGGFFFFBFFA?F?GFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:90:8728:2691/1 0 chrIV 11034 60 101M * 0 0 AGATCTGTGATAGAAAGACGAGGAGGAGACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCG GGGGGFGGGGGGGFDGGGGGEFFGGFEFGGGGFGGG:AGFGGEGEGFFGGEGEDGGBFGEFGEGGGGEGEDEEGEEEGBEEEEEEACAEDDDBDEADB?BD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:58:9805:10604/1 0 chrIV 11061 60 101M * 0 0 GACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCGTCTCTTCTGCCTCTTTTCGATTTTAAA GGGGGGGGGGGGGGGGGGGGGGGGFGFGGGGGGEGGGGGEDGGFFGGFGGGGGGGGGGGGEGGGGFFFCFFDFFCGDGEDGFEGGFGEEEDGE=DEDDDCE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410 99 chrIV 12069 60 100M = 12243 274 GAATTGATTAGTCAAAACCTTACAACTTGTGACTCAGAATTTTGAAAGTCTACCGAAAAAATAACGTTCTTCAACAATTACGATTCTTCAATTCTAATAG CCCFFFFFHHHHHJJJJJJJJJJJJIJJIEHGIGGGGIIJJJJIIJJGGIGHIIIJJIJGH2BEGH?EEFF>DEECDEDEC@B?ACCDCD:;ACDACCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:23:5518:6512/1 16 chrIV 12134 60 101M * 0 0 GTTCTTCAACAATTACGATTCTTCAATTCTAATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTA ?CBAC=CA@:AEBBBD=FCEEE?=EECBA?CBFDFC>:EE=:C:EEEEBEE5FAEF=EEEEDEEBEFBFFDFFFFFFBFEEFFBDFFADFF?DDDD5ACC= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:9:17295:4977/1 0 chrIV 12165 60 101M * 0 0 ATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTATTAACAAATTACAACAGTACACTTGGTGTCA AEECDEEEEEDGGGGGFGGGFGGGFGGGFFDEEEEGGGGGGFG?GGAFGGGFGGGFFGGFGGG=GEEEE?E=EEEFFGDDGG?AGABAD?=CEBEEADBEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410 147 chrIV 12243 60 100M = 12069 -274 TTACAACAGTACACTTGGTGTCAGAGTGTCTTATTTCGGCTACAAAAAATGCGGGTAAAGAAACGCAGAGTTCTCAATTAATTTCGCATGGTTAAGAACG ACCCDDCDDDAC>DDDDDDDDDDCCCDDDDDCDBBAFFEFFHHHJJHJIIIJIJJJJIIIHFDHJJJJJJJIJIIJJIJJJIJJJJJHHHHHFFFFF@CC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300 99 chrIV 12490 60 100M = 12628 238 CTGCCGGCCGTAAAAAGTCATTCACCTCATCATCCGGCGATCTTCTCAATGCTCCAAACCGCACTAATGGACAATCCCGAACACCACCTGCTCCACGAAA CCCDFFFFHHHHHJJJJHIIIJIJJJJJJJJJJIJJJJIIHHHHHHFFFFFFEEEEEEDDDDDDDDDDDDDDDDDCCDDDDDDDDDD@BCDDDD@CB9@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300 147 chrIV 12628 60 100M = 12490 -238 GAGCAATCGGAGATGCTCTGCCGACTCTTGACGGCGATAATCTCATCAATATCAAGGCGATCTCGGGGCTTCATTTGGATGAATCTGATGAGGAAAAGGG DDDDDDDDDDDDDCAB?DBDDBC@BDDDDDFFHHIGJJJIJJJJJJJJJIIGJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:57:18691:3342/1 16 chrIV 13429 60 101M * 0 0 ATTCAATTTTCGGCAAAAAATAAATTTATGATGAACAAGTTGAGATTAAAATATAACTTTTCCTCTGATGAAATTTTTTTATTTTATCAAAAAATCGGAAA BBB=FDFFF?FAE;C?EGGGGGGFDGDGGDFFEGBGDEGGGGGF=GEGGFGFGGGDGGGEGDEGDGEEECDDDGGGCCBEAEFFDDDDDE=FFAFFGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:10466:54597 99 chrIV 13926 60 95M5S = 14386 560 GTTCAAAGGCCCCCTCCGGCATACTCGGATCAGCATCATGCGAGTTCGACATCTGCTTCGGCTCCGCTGATATCGGTTATGCTTCTGAAGAAGCCGATGG C@@FFFFFGHGHHJJJIJJJJFAFGGGIGIIIJIJJEIJJJJFIADHGEHBDFEFFFEDDDDBDDDDDBDCCACDD?3<CCD@AACACDDDDCCB##### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:15940:55697 99 chrIV 14066 60 100M = 14107 141 GGGCTGTGCGGCTACCGAGTTGCCAAAAGTTGGGGTTCGGCAACTTCGGCAATTGCCGGTTGCCGAAAATTTTGGAGTTCGGCAACTTAGGCAACTGCCG CCCFFFEFHHHHHJJJJJJIJJJJJJJJJGIIJJJGIJJJHJJHHHHHFFDDEDDDDDD=BDDDDDDDDDDDDCDCC>CDDDDDDDCD@CDDDDDDDDDB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:15940:55697 147 chrIV 14107 60 100M = 14066 -141 AACTTCGGCAATTGCCGGTTGCCGAAAATTTTGGAGTTCGGCAACTTAGGCAACTGCCGATATCAATGTCAAACATTTTGTAACCCATAAAAAAACATTC @<BBBDDDCDDBBDDDDABBD@?CDDDDDDDDEEDDFFGHGHJIIJJIHFJIGJJJIIJJJIJJJJJJIIJIIJJJJJIJJIHJJJJHHHHHFFDFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:10466:54597 147 chrIV 14386 60 100M = 13926 -560 GGTCGTCTAGCCTATCTGAGATTGCCTACGCTCACCTTCATGTTGGCCGAGCCTAAACTCTTACCTACGCCTACTTTGTACCTACTGAGCAATCTAGCCT BBB<>><>(@>@C>3>ACDCA=;5BB=9=3?7;7CHHHHG?GIHGGGHDBJJJIJIGIHGEB?JHF?IIHHCFJJIGHHEJHEIJIGHGHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:45:7639:19200/1 16 chrIV 15054 60 101M * 0 0 CGTGTCAATACCGTTGCCTTATTCTCTTCAAATTTCTAATTTGTAACAATTTTCACACACACACTTATACAAAAATTACGGAAAACTCTCTTGAAACAACG C?BBBBBA@>EFEBDDBB=EABBFAED:FF=EEBBBAE;BE=E?EAEBDBBEBDBEBEBEBEAEBBFC=FFFFECDFDFFFBGGEFFGFGGFFFFEEECEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:32:13629:16208/1 16 chrIV 15294 60 101M * 0 0 ATACAATATTACTGTGATAGAAGCACTGAAAATTCTCTTTCATAAAAAAATTATGTAATGCGTTGTTGTTTGCGACAGACTTTTATTTATATATGACATTA DBDGGBGEFFEFFGGEDGAGGFGGEGAFAF?DGDBGGFGDGFGGGDGFG=GDGFGGEGEGGFGGGGGGFGFGGGGGGGEFGDGGGGGGGGGGGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675 99 chrIV 16174 60 100M = 16449 375 AGTATAGGTTGAGTGTCATATTCGAAAGTGGCCTCCACTCCTCCAAATATTAGGTTATGGATTTATCTGTGCTGTTTCACAAGAAATGCCCAAGCTTCTT @@?DBDDFFHHDDHIBGIIEHJJJEGIG<FHBEHIJIIGGIBFEGGGIJIJIIGFFGIJIIIEHGIIGCFHGDHIGIHHGHFHFFFFFFEEC=ABDDDCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:86:18150:16897/1 0 chrIV 16311 60 101M * 0 0 TAACTCTAGAGCCGTTTGACTGAATTTTATAATATTTGACAGCTTACTAGAAATATTTCACCCCACGGGGGAGCAAAAAACTGCACAAAGTTTGGAGCAAA =EFGGDGBGDGDGGGGGGDGFEF=EFFFAFAFEFDFFEFBF?FBFFFFFDFFFDFFFFFFFFFFEF>FBFB=>9B5??>=F@DFDEB=?B?B@?B@:@@=B PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114 73 chrIV 16371 60 100M = 16371 0 CCCCACGGGGGAGCAAAAAACTGCACAAAGTTTGGAGCAAAACTGAGCTCAGGACGGCTTTCCCCAGTTTGAAAAAAAATTTTGTTGCTCTTTTTACCCC @@@FFDFD8CFFHGGGIIJB@HGJFIIJFE.@?ECHGFFD@CEEDECDC;5?CABB@B;5?>@ACC@ACC@:ACCDD9BB@>AAA98:>CC@CCC?>>38 PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114 133 chrIV 16371 0 * = 16371 0 AAAATTAGTTTTTCGTCTTTATCTCAAGTTCTTCTTCATCTTTTTCGATACTTTTTTTTTCTACCCCACGTAAAAAAGTACGCTGAACACGATTTTCAAC :@:4(:4<@9<B<3CCC@:>@8>::>:?>>99(@>>>:?@?BBCB?;>5(BCEFIGHGB=67(HED;?BBEIIGDEF@<3<@<?9GF?AA=<DDDDD@?@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:19827:42088 73 chrIV 16422 60 100M = 16422 0 GGGCGGCTTTCCCCAGTTTGAAAAAATTTTTTGTTGCTCTTTTTACCCCCAAAAAACCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGT BCCFFFFFHHHHGJGHGHGEHIJJJJGGJJJJJIJJIJHHHFHHFFFFFDC@DDDDDDDCD>CDCDDCDCDDDECDDDDDCBBDABDDDCACBD?>@DBA PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:19827:42088 133 chrIV 16422 0 * = 16422 0 GCCTTTTGTTTTATCACAACTTTTTTCCATTTGCACTTATGAACTCAATCTTTCTTTCAAAAAATCAGTCTCTCTGAGTAGTATCTTGCACAGAAATTTG CCCFFFFFFFHHGIIIJIJJJJJJJIJJEIIJGIGHGHIJJJJJIJIJJJJJJIGIGIJIEHIIHIIGGHHHHGHEFFBBDBDEEEEEDDDD?CDACCDD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:8211:57172 165 chrIV 16449 0 * = 16449 0 ACATGAAATAATGCGAAATGGATTTTCTAATTTGTAGAAATTATAAGATAGCTCCGAATTCTGCCAAGCAGCCTCTAATAAATGCCGAGTGTACAGACTC ?BBFA=ADHHGHHJJJJJJIJIJJJIHIJIIJJJIIJJJJJJJJIJIJJJJIJJIJJJJJJIJJIJIJEIIHHHHFFFFFFFEEDDDDDBDCDEEECBDC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675 147 chrIV 16449 60 100M = 16174 -375 TTTTTGTTGCTCTTTTTATCCCCAAAAAACCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCAAGATACTACTC 8DDBBDDCC<?5>A:><:@BBB@>;;@>?>FHFFHGHIIHGHBGGGIIGFHGHGGB;GEH@0EGE7IGGHGCEF@BHJI<HGBGA9HBFFFC=:BDD@?? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:8211:57172 89 chrIV 16449 60 100M = 16449 0 TTTTTGTTGCTCTTTTTACCCCCAAAAAAGCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTTTGTGCAAGGTACTACTC 8BBB?DDCDDBBCDDDDBDDDDDDEEEEEFFFDFHGHHFHHJJJJIJIIHIJJIIJJJJJJIIIJJJJJJJIJJJJJJJIJJJJIJJGGHHHFDFDFCB@ PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:12400:9160 99 chrIV 16457 60 100M = 16639 282 GCTCTTTTTACCCCCAAAAAAGCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCAAGATACTACTCAGAGAGGT CCCFFFFFHHGHHJJJJJIJJJIJJJIJJJJJIJJIJJJJJJIJJJJJJGCGIJJJJJHHGFFFFEEEEEEDDDDEDFEDEDDDDDCC@CDDDDCCDDD3 PG:Z:SNAP RG:Z:000 NM:i:2 +EAGLE:1:46:17265:14051/1 16 chrIV 16476 60 62M3D39M * 0 0 AACCATGTTTTAATTGAATTAAAGTTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCATACTACTCAGAGAGGTGGATTTTTTGAAAGAAAGTTTGA D<>A7?@C==EADFFGFFBE?B?D@=CFFFEFAGGGGFGGGEDEBBBA?BFFFFEDC?DDB?C>C?CA=:=EDDBDGFFGGGGFDGAEEFDF5FFFGDGFB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:94:17078:10619/1 0 chrIV 16610 60 33M1D38M1D30M * 0 0 ATAAAACAAAAGGCCAAAAAATGACATTTTGCCAAAAAAATTTTTTTTCCGAAAAAGTAGTTTTTCGTCATATCTCAAGTTCTACTTCATCTTTTTAGATA GEGGFGGGDGFFGGGGGGGFGGGFGBGDEDGGGGGGGGGGGGGGGGEEGDGGGFEEFCDECADEBFG@EDAEEBEEC?ABDEDDFEEEEEA?DDBC?BDC@ PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:12400:9160 147 chrIV 16639 60 1M1I18M1D80M = 16457 -282 TTGCCAAAAAAAAATTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTTCATACTTTTTTTGTTTACCCCACGTAAAAA DCDBDDDDDDDDDDDDDDDDDDDDCEDDDDDDDDDDDDEEFFFFFFHHHHHHIIEECIIJJJJJJJIJJHHHFDGJJJJJJJJJIHEHHFFHFFFFFCCB PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100 165 chrIV 16645 0 * = 16645 0 AATTCACACGGTTCAGGAGTTGGAAATAAAAACGTGGTGTGACATTTTTTCGCAAGCCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCTGTA #####################################################B@A;/DDFHIGG@GIEIIJIIHFIIJJIJJJJJJHHGHHFFFFFCB@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100 89 chrIV 16645 60 76M1D24M = 16645 0 AAAAAAATTTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTCGATACTTTTTTTTCTACCCCACGTAAAAAAGTACGC >BCDCA8@@BCB?<50+CDDCC@C@BDDDB@@CDCDCADBCCDDDDEC@;9EEEDFFFHHIIHHEEF=GJJJJJGGGGD?IIGHDIGGHHHHFFDDD@C@ PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:2:31:3792:4681/1 0 chrIV 16705 60 101M * 0 0 TTTTGAGATTTTTTTTGTTTACCCTACGTAAAAAAGTACGCTGAACACGATTTTCAACTCAGAATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAA FBFFF?GGGGDGEGFEGGGEGGGGDGGGGGGFFGGDEGGGDFGFGGFGGGDDFDGGGGGGEEDEGGGGGFGGDEBDFEFFGF?DGEFGGGEGBBEFEEEAE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:48:7607:3697/1 0 chrIV 16768 60 101M * 0 0 ATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAATAAGTGCCAAATTTTGCCCACTTTCCCGTTTTCGCGAATCTACTTTTTCCATCATAACTCGGT GGGDDGFFGGGGFGGGFDEGEFDFFFF?DFFEFEDFFFFDBDADCFFFFFFG?GGGGFEFGGGGGFBD:BEEEBA@EEBED@?DDDDEDADBD:DB=ABB8 PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377 73 chrIV 16778 60 100M = 16778 0 GTTTTATGAGTCGGCCGAGCAAGACGAATAAGTGCCACATTTTGCACACTTTTCTATTTTCGTGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCA BCCFFFFAHFHHHIFIIIJJJIIIJJIJIJJG>FFHIIJIIIIIIGIJIJGCGIHEHHHHHFFFEAECEACCCDDDDCCDDDD@CCDDBB@B@C:>@CCD PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377 133 chrIV 16778 0 * = 16778 0 ATGAAGTTTCGTCGCATTAGAAATACTCTGCCAAGAAATATTATTTTTATTAATTTTCAGGCCAAAACTTGTTTTTTTCTACAAATCGACCGGTTTACGA CCA@>DDDDDB?BACCDDDCDDC@@DDDDDDDEEEEFEEDFFFFEHHHHHGHJJJJJJIHFGGJJIGJJJIJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:2:84:15670:14903/1 16 chrIV 16791 60 101M * 0 0 GCCGAGCAAGACGAATCAGTGCCGAATTTTGCACACTTCCCCTATTTCGCGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCAATTTTTCTTAGTTT BCADAEB?CCBDFDCDBEEBGEGEEEEECECFCD@FEDFGGEFFEDDFFFFBGEGGDFFFFFGGGGGFGGGFGFGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1106:14433:6441 165 chrIV 16828 0 * = 16828 0 ACCGTCGGCTGAATTTATAAAAATAGACCATGAGAGGAATTTTTTAAGTCAAACCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCACCACAC ######################################################################GB:0))*?C9;GE@+C<?HD?=++=DD;=8 PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1106:14433:6441 89 chrIV 16828 60 5S95M = 16828 0 ACACTTTCTCATCTTTGCGAATCTTCTTTTTCAATCATAACTCGGTCAGTTTTCAAGTTTTCTTAGTTTTCTAAAAATTGACGTGTAGGTCTTATCAAGA #####CCCCCCC>?=385;(@>6.(46;@C=:ACAEHHE;@6FCEDBIGCHD<GFD:9HCFF@HGIHFFBF=IHHFFCAACFBEA@BAFHDHD;A1D=:8 PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:16001:53612 99 chrIV 16842 60 100M = 16921 179 AATCTACTTTTTCAATCATAACTCGGTCAGTTTTCAAGTTTTCTTAGTTTTCCAAAAATTGACGTGTAGGTCTCATCAAGACGCATCGAGACATATAAAA CCCFFFFFHHHHHIJJJJJJJJJJJJFHJJIJJJJGIJGIJJJJJIJHIJJJJJJJJJJJJJJJHIJJIJJJIJJHFHHHHFFFDDDDDDBDCDDEECDC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:2:76:14252:18422/1 0 chrIV 16876 60 101M * 0 0 CAAGTTTTCTTAGTTTTCCAAAAATTGACGTGTAGGTCTCATCAAGACGCATCGAGACATATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAA DDDADBFFFFGGEGBGGGGFDDGBGGGFGDCFFDFFDFFFAEEEEGGGEGGGGDGGDGGGGGDGGEDGEGGGDFGGGBDGFFBFFGEGGBDBBFFG=DG?F PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:16001:53612 147 chrIV 16921 60 100M = 16842 -179 GACGCATCGAGACATATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGC 0BDDEDDDDEEEEEEDEDDDCDCDDDDDDDDDDEEDEEEFFFFFFHHHHHJJJIJJJJJJIFIGIHGJJJJIJJIIHHFJHJJJJJJHHHHFFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:16910:75714 165 chrIV 16922 0 * = 16922 0 GGTCAGTTTTCAATTTTTCTTAGTTTTCCAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGCTTATCAAAAACAAACAGAACGAAAAAAATTTTTAAAAAAT @C?DFFDFHHHHGJJJJJJJJJJIJJJJJJJJJJJJJJJJJHFDDDDDDDB################################################# PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:16910:75714 89 chrIV 16922 60 100M = 16922 0 ACGCATCGAGACGTATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATTATTCAAAAATTTAGAACTGGGGGGAGTGGTTTCCTGGG <DDEDDDDDDDDDDDEDDDDDCDDBDDDDDDDDEEEEEDFFFFFHHHHFGJJJJIEJJJJJIIJIIJIJJIJJJIGJHJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:6 +EAGLE:1:116:7753:12206/1 0 chrIV 16940 60 101M * 0 0 AATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCCCCTGAACATTTTATCC EBE=EAEEEEFBFDEFFFFFFF;EFAFFDFFFFFDFFF@ECCCCACDFFFEFFFFFFFAFFEF=FEFFBC<FF@BCACBAEE=EBEE?CBEDD?EEA=B7C PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:78:11145:14387/1 0 chrIV 16952 60 101M * 0 0 GTTCAGTGGGAAAATTTTTCAAGAAAAAAATTATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAGCATTTTTTCCACTAAAGTTTTG GGGGGGFDDGGEGGGGGGGGFGGDFG@GFGDFGGGGDGGGFGFGEGEGFDGGFEGGCEE:@>>>CDDDED:CDCDDC?DEEEEEEEEBEEBBCCCEEEEE? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:43:19134:12725/1 0 chrIV 16956 60 101M * 0 0 AGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGA FFFFFGEGGGFGGAGGGFGGGGGGGGGGGGGGGGGGDGGGDFEFFGGGGGGGAD@FFCDFGGEFGFEGFGGFGEEEEEGEGGGEGBGGEEBE@DBEBECEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:57:3806:20180/1 0 chrIV 16957 60 101M * 0 0 GTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAA CCC:;DD?BDEEDDEEDE=DECECCEDEEEEAE?EEDEE=ACACCBA=ADDA@;DEBCEEEEEEBBE:EEEEEEEBEDEDEEBEEEB?A@C5@@CC?BD?5 PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:28:11193:2776/1 0 chrIV 16966 60 41M1D59M1I * 0 0 TTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGAGTGGTTTCCTGAGTCCCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTA GGGGGGGGEGGDFGGGGGGGGGGGGGGGGGDGGDFGGGGGGGG?GFCFFFGGGEGEGGGGGGEGFEGGGGEEEGFEEEEEGEEFGECEEA?CECCDEEECE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:63:18448:7383/1 0 chrIV 16991 60 101M * 0 0 AATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTAATTCATAGTTTTTGATTTTATCTA FDGEGGEGGGGGGFDGGGCGGGEGGGGFGGEAEEEFFDFFFDGGDGGFGGGGGGGGFEGFFFFFFE?FEBBEEEEACEFDFFFAFEGFGGGBFBGDDDGCC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705 73 chrIV 16997 60 100M = 16997 0 GTACGGGGTGGAGTAGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAATGTATTTTTGTTTCATAGGTTTTGATTTTATCTAATGGA BBBFDDFF@DHHHDHIHIIJJIJJJCGHJJIHJJIIIJJIJJJIJJJJJJCEHIJIHHFEDDECDDFEEDBACDDDEEDDCCDDDBCDEDEDCDECDDCD PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705 133 chrIV 16997 0 * = 16997 0 GTTGAGGGCTATATCTTGTAAACTGGTTTATTTATAGAAACAAACAAGTTTTGGTCTGAAAATTTTGAAAAAATAATATCTCTTGGCAGAGCATTGCTAA CCCFFFFFHHHHHJJJJJJIJJJJJJIIJJJJJJJJIJJJJJJJJJJJGIJJJJJJJJJJJIIJJGHIJJJJHFFCDFFFFEEEEDCDBBDDDDDCDDEC PG:Z:SNAP RG:Z:000 +EAGLE:1:53:14974:4822/1 0 chrIV 17018 60 101M * 0 0 TGGGTTTCCTGAACATTTTTTCCACTAAAGTTTTGCAGAATGTATTTTTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTAACATTAA AABA:=DDDDGF?GGGGGGEEFGGFEDE?E?DDDAE=EBEDEEDEEDEB@?DEDBEBECEEE=EAGDGD:FFCB?FDADEGAFGBFDAEFEEA?E?BAA?A PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:79:7894:12928/1 16 chrIV 17065 60 101M * 0 0 TTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTGTCATTAACCATGCTCTGCCAAGAGATATTATTTTTTTAATTTTCAGGCCAAAAC ECCCDCBAE=DEEEEEEEEEEEDDEE=EEGGEEGGGGEEGDFGGFGFEEAFCGBGGGEGGEGGGDGGEGGGGGGGGGGGGGGGGGFGFFAGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:3180:43522 73 chrIV 17244 60 100M = 17244 0 GAGTACAATCCTGTAATAAAATTTTCAAAAATTCGTTTTCCCGCTGACTAATCAGTGATAAACGGTTTTCAAACATATTTTCTCAAATTGTCATTATTGA BCCBDEFFHHHHHBGGGHIJJJIJJJJIGFFA<AGFGHIJIJJJJJJJEIHIJJJHIJIIIIJIJDEFHHHFHFFFFFFDEEEEDECDCC@CDECDDDCC PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:3180:43522 133 chrIV 17244 0 * = 17244 0 TTATTATATAATCCTTAACCGTTCACATTTTTGTCTCATGGGAAGTAAAACAGTTCACTGGTTGTTACTACAACATTTATTCTCAGCTTCTCTCTACAGA CEEDEDEDDDCCCEEDDDFHHGHAHIGIJIGFJIJJIEJJJJJIJIIIJIJJJJIIIIJIGJJIIIIJIHIJJJIJJJJJJJJJIJJGHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:3598:47398 165 chrIV 17460 0 * = 17460 0 GGCTTATACAAGGACTGAAGTTATATTTTGAAATTTTTCAGCTGATATAAGTGAAAAAAGGAAGCATAAATTGTTTTCGCAATGTGCCCTTTATGCTCGA CCCFDFFFHHHHHJJJJGIIHGIJJJIJJDIJJJJJJJEHIHJJIIIJJJIHIJIIIIIIIIJJJJEGJJBHEEEAEHE9AACCACDDDDDCDBACDDD? PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:3598:47398 89 chrIV 17460 60 35M1D28M2I35M = 17460 0 GTTGGCTCGATTCCCCCAACTGACAAATTTTTTTTGGGTAATTGGGATTCGAATAGAAATGCTCGATTTTAAGCAAAATATGAGTGGGTCTCCTCTCCTC BDDDDDDDDB@5B@DCCDCDEDCCCCDBDEHEHEJJIHGGIGHGHEHGEBIGEDGGIJJJIGIHIGJJIDJJIIJJJHBHEGGGIIJHGHGHFFDDDCB@ PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:1:76:16063:11662/1 0 chrIV 18079 60 101M * 0 0 AATATACAAGAAAGAGCTCTCTGATGAAAAAATTGACCCTGAAATAAAAAAAATAGTAATTTGGTACTTTGCAAGGAAGACCTACTTGATATACTGACATG GGGGGGGGGGGGGGGGGDGFGGGGGGGGGGFGGFGEGGGGGGGGGGGGGEEEEDEGEGGGBFFFCGGEEGGGEEEGEDGEEEEEGEEEEEEEEEEEEEEED PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:58:12937:19702/1 0 chrIV 18223 60 101M * 0 0 AACATTAAAACTGTCTTTATCTGAAACATTAAAAGATTGGAGTAATAAAGAACAATGTGACGGATATATTCAGTCATGATTTGATTACCGACTGAGCTGCT EGGEGEGGGGGGGFGGEGGGGGGGGDGEGGGGDDGCGGGGGGEGFGGFGFGGGGGGGEFFGDGFEFGGGGGFGEGGGGEEEGGFEGGGFGEEGGEF=AGBD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:42:10933:8029/1 0 chrIV 18242 60 101M * 0 0 TCTGAAACATTAAAAGATTGGAGTAATAAAGAACAATGTGACGGATATATTCAGTCATGATTTGATTACCGACTGAGCTGCTATTACCGACTGAGCTACTT FGGGDGGGGGGEGAGGFGGGGGGEFDGDGGFEGGGGGGEGGGGGGGGFEGGGGGEGGGGEFGGD=DCDFFGFGEFEGFEGFGEGBGDFGEBGEDEDEEEEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:57:16376:15904/1 16 chrIV 19300 60 101M * 0 0 ATCTCTTTTCTCGTAACTGCTGTAAAAAGGAACGAAGCACAGCATATAGGCAAACAGAAAAATGAATAAGACTCGGAAGATCTGGAAATAACATTATTATT :DFBFEDEBGFFEGGEGEGGGGDGG?FGEEDEGGBGGDGEGGEGGGGGGGGGEGDGGGGGGGGGGFGGGGEGGGGGGGGGGGGGGGGGEGGGFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:82:4792:16460/1 0 chrIV 19447 60 101M * 0 0 ATCAATGATTTGTTACAAGAAATTTTAGAATTTGTCCGACACAATTCTTAGATTTTTGAAAATACTGTTCAAACATTATAAAAATATATTTAAGTTGCACT DGGGFGBFGGDGGGFGGFGGGGGGGGGEFGGFGEEGGGFGFFDGEGGGCGGGFGGGGEEGGGEFGBFAEFGGFGEEEFEEEEEE>C@EDEEEGEECEF=@C PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856 99 chrIV 19979 60 100M = 20074 195 TCTTGATACTGCATGAATTTTCTTTCTGCCAAAAATGAAAAAGTTGAGGCATAATCAGCTTTCAATGCTTCCATCGCTTGACGCGGTCCCTTGACTCCAT BBBFDDDFHHHHHJIJIIIJJHIJJJIGIJIJJIJJJJJIJJIGHGJIJJGHHIJIJGBGIGIIJJJJJJEHHJJJHGHEFFFFDDBB;BCCDDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856 147 chrIV 20074 60 100M = 19979 -195 TCCATTCAAGTTGAGATGATTGATAACTTTACACGCGAGTTCTTCAAGTTGTCTTTAAAATCAGAAATGGCCGTACACTTGCAAACGAGAAAACCTACTC AC@DDDDDCDDDDDDDDDDDEDEDCDCCCCAEEGJJJJIHEJIJJJJJJJJJIJJIJJJJGJJIJJJIJJJIIIHHCJJJJJJJJIJHHHHHDFFDBC@B PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661 99 chrIV 20173 60 100M = 20424 334 TCTTGAGTTGAGCGTAATCATTGCGCTTTAAAACTTGCCCGAAATCCTTAGCGTCTAATAATCGTTTCATAGAATGCGCCACGAAGCACATTACCATTAT ?@@DDBDADDHFBE<CEBHBHICH?FAEH@@?CGH@F<9BFEF@HGECEGI>B';AEFEFEHC?;B566>;;>6>;5'9''3;?5?9CCCCCACCC>:A3 PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661 147 chrIV 20424 60 17S83M = 20173 -334 AAAAAATGACGACACCAACACTTTGAATTTAGTACGTACTCTTGCTTTTTGCATTGCAAATTTTTCTTAATTTGAATGTTGAGTTCATCCTTTCTACAAT #################@;)CHC;CC=:ED@EECHDCB2G>F?(0)?9?9E??:IHDH?);HE9BEFBH@IHFA<3CFFA3322++<++:<HFDB=BBB8 PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:75:4143:14945/1 0 chrIV 22397 60 101M * 0 0 GTTCAAATCAACCAAAGTTCAGTTACGCAGTGTAAAACTGGATTTTGACCATGGTGCATCTATTGAAAGTTTGTATATTGTCTCACCAAAAAATCGTTTCA GGGGGGDGGGGGGGGGGFGGGFFGDFEFAFEFFCFGGGGGGFGEGGAEGGGFGGCFFFEFGEEGGEEG?@EFADEFGEEFEFEGEEFEEDFDCBEE>C=E@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857 99 chrIV 22773 60 100M = 23094 421 ATACAGTATATTAGTTTTTAAAAGTTTTGTGCCAAACGAAATTTTGTCGAAAGTTGACAACTTTTTTGTCTAGAATTGGCAATTTTTTAAAAATTTAAAT CCCFFFFFHHHHHJHGIJJJJJJJJJJJJHJJJJJJJJJJJJIJJJIJIIHIIIIIJJJJJJJJJJHHHHFFFFFFB@CEE>ACCDDDDDDDDDDDDEDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857 147 chrIV 23094 60 100M = 22773 -421 GTTTTTGAATTGAAATTGAAAGAAATCGAGCAACTTGAAGCCGAGGCCGAAAACACAATTCCAAAAATACCCAAGAAAGCAGCTGGCTCATCTTCAGAGC @DDDDCCEEDDCDEEDCDDDDDBCA<DFFFFFDHHHHHJJIJJJJJJIJJJJJIHJJJIGJJJJJIHF?JJJJJJJJJJJIJJIJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:16786:19522 99 chrIV 23918 60 100M = 24045 227 GTAATGTACGTATCAAAAATTTTTCTATTATATATTATTTATAGTAATTTGTTTGCGACCCTATCGTATTTTAATGAAGGATGTAAAGTAACAGACTACA BBCFFFFFHHFHHJJJJJIJJJJJJJJJJIJJJJJJJIJJIJJJIICIIJJJJJJJJJJJJJJJJJHIIJJHHHHHHHFFFFFEEEECCEDDDCDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:16786:19522 147 chrIV 24045 60 100M = 23918 -227 CCATTTTGCAAACTTTCAGTAGAAAATTTTCAACTTTCTAAAGCACAACTTTGAGTCGATAACAAACAAAAAAGATATCCCTCATTTATTTTTCCACACT ADDDDCDCCDDCDDDDDEDEDECDEFDFFFFHHHHHHJIJJJJJIJGIHIJJJJIJJIJJIHJJIIIJJJJJJJIIIIJJJIJJJJJHGHHHFFDADBB@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:49:4402:14093/1 16 chrV 2002 60 101M * 0 0 ATTAGTCTTGCATGCAAGACTAATTTTCAATTGACCCGTAGGGGTGCAAGACAAATAGGGGTGCAAGACTAATAGAGGCTGCAAGACTAATAGAGGAAATA DBEDEEDCCEA@DEDDEGEEGDDEED=FEDGEFBEDFGFEGGGGGGEGFEECGFGGFFGGGGGFGGGEGGGGGFGGGG=GGGGGGGFGGGEGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:42:2639:3086/1 16 chrV 2123 60 101M * 0 0 GCGATAGCATTTCGAGAGAAAAATATTCTTAAATGTGCTCAATATGGCTGATACAGATAGGTTTTTCATTACAGACAGGTTTTTCATTTCAATTTTAAAAA EBEEEBEEB:BEBEEA;A=EEBE?CEE?EEEEED?D?EEE:DDEEEEEEE=DAD:DEE=DEBEEAEEEDEEEBDEBDEEDEAEEEEGGEGFGGGGD;FD?F PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:20321:98700 99 chrV 3178 60 100M = 3335 257 GTCTATGAAAATGCTTTTATTTGGTATTACAGTTCTTTTCAAAGGCACACATATTTTTCCCATTTAACAAAAAATCGTCGTGACCTTATATCGTATTTTC CCCFFFFFHHHHHJJJJJIJJJJGCHIJJJJJIGIJJJJIIJJJJJJJIIDHIJJJJJJJJJJJJJJJGHJJJJJGHFFEDDEDECDDDDFEDCDDDEEE PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:20321:98700 147 chrV 3335 60 100M = 3178 -257 GCAATAATACAACACCGGGTCTCGACGTGGCAAACGATTGTTTATTGATTTTCACGGGATTCTCGCCTTTCTCATTGAATTTTCGCGCTCCATTGGCAAT CDEEDEDDCDD@@<DDDDDDDDDBBDDDDCDDDDDDDDDDDEEEEECFFFFFHHHJJJJJJJJJIJIJJJJIJJJJJJJIJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:16390:41463 99 chrV 3761 60 100M = 3998 337 AAATAGAATTCATGATACACGATCTTGAGAAGAGCTCTGTCCTCCAGGCTGCGAACTGGAGCGTAGAAAGTTTTCATATTGTTAAAAACTGCAAAGAAAT CCCFFFFFHHHHHJJIJJJJJJJJJJJJIJJIJJGIIJHHIJJJJJJJJIHIJJJJJJJIJJHHFFFFEECEEEDDEEFEEEDDEDDDDDDDD@CCC1?> PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:71:9405:4095/1 0 chrV 3773 60 101M * 0 0 TGATACACGATCTTGAGAAGAGCTCTGTCCTCCAGGCTGCGAACTGGAGCGTAGAAAGTTTTCATATTGTTAAAAACTGCAAAGAAATGTTCGATTCCTTG DA=DADDBB:;?@@@=DDDDDDD?DDDBDDCDDDDDDDDDDB?C=DDADDD=DDB?AA:ADCDDCCBCDB=:@ACDBCA=ABCBDB@>A@ABC??BB=BBB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:65:16164:2544/1 16 chrV 3846 60 101M * 0 0 AAACTGCAAAGAAATGTTCGATTCCTTGTTCTGACGTGTGATGCTTTCTCAAATATGGAATTAAATGACAGACAAGGAGATGAAGTTTCGGAGTGACTCAT @?@C@B;>EDDBDDBEEEABBA@B=C:EFEFFDBFEEEEEEADDCBEDBDBFD5FFFFFBFFFEBEFEAFFFAFFFFFFFFEEDEE?D=GGGGFGGGFGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158 99 chrV 3997 60 100M = 4220 323 GTTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTAAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTA C@@FFFFFHGHHHJJJJIJJJJJIIJIJJJJJJJJFHIJJJJJIJGIJJJIJJJJJJJJJJJJJJIJIIIJIIIIDGDHHEHGFFB>CCCEEDDDDEEAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:16390:41463 147 chrV 3998 60 100M = 3761 -337 TTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTCAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTAA DDCCC>DC@ECEEC@EEFFEFHHE>HGIIIGHCEC=/)FIHFHIJIIGIHIJJJIHIIIJJIIIIGGJIJJJJJJIIJIHIJJIIGGHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158 147 chrV 4220 60 100M = 3997 -323 AAAATTAGTTTAATCTCCTCGCAAGATTTTTTTCTCCGAAATTTAAGATTGGGCGCTTAGTGATATCATAACTCTGCAGCCATCAGAAAAACTGAAATGT CDEDDDDCCDCCA@BBDD@BC>CCDB@CEFHHFIJIJHIIIGJIJIIIIHJJIJGHIIJJIGJJIJIHGHHGFJJJJJIJJIEJJJJHGHHGFDFFF@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:76:4095:13262/1 16 chrV 4714 60 101M * 0 0 ATCGTAAGGCGGTACTTTTTAAAATTTCGTTTCCATTCTTCCCCAGGGATCCCGTAAAAAACCAATTTTTCGACTAGGTCTCACATTTTGCCCTACAAGAG B=BC:CACBCACA=ECD:CDGA?AA:ADA:A@EEEBCGDAGGGGEGGGEDGGGGFFGGFGBFFFFFFGGEGGGGEGGGGGFGDGGGGFGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:89:14779:18797/1 0 chrV 4998 60 101M * 0 0 TGTGTTTGGTTTCATTCAGACGCTACTTGCCTGATCTATCAGTCTAACAATTTTTGTTTAATTTTCTTCACTATCCGCCGTTTATAGTTCGTTCTTCCGTA EEE=FEFEEEFFGGGFGGFGGGGDGGGGGGGFD?DFFEFCFGDGGEGGEEGGGGGGEGG=FGGFFFGEEGGFEGGEGGGGEGG@EDDADDEEEBGDDEDCE PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201 99 chrV 5929 60 100M = 6103 274 CTCCAGGAAGACGAAAATTCCACAGACTGAGTATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGAT CCCFFFFFGHGHGJJJJJJJJJJJIIJIIGGCGIIJIIJHIJJDHIIJJIIIJJJJJJJJJJJHGFFFFFFFCEEEEEEDEDDDDD=BDEEEDEDDDDCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:16987:87976 99 chrV 5959 60 82M18S = 6130 228 GAATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTC 1++=+2+22+2A:HD?E,A+++32+++2A@*:8C):BBGD4*9B9*?*099/?6(6@'.@E;)).==)7;7?),5;ACA53@################## PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:85:10419:2317/1 0 chrV 5963 60 101M * 0 0 CCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTCAATGG GGGGGGGGGEFFFFFBDGGGGGGGGGGGGGGGGGEGGDGGGGGFEFGFEFEEBEGFDGEEGEGGBEGEBGGDEEEEBEEE?DCDCEE?EECE;CCACCCC? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201 147 chrV 6103 60 100M = 5929 -274 CACCTATGCGTCACGCTATGGTCCCGCATATCTTCGCGAACGATTCGGAAGCCATGATTCCCAACTTGAGCGTATGCTTTATGGCGGCAGTGGACCACAG @:CCDDBBDDDB<BDDDDDDDDBBBDCC@CDDDDDDFFFEHHHJHIJJJJIJJJJJIGHJIGIJJJJJJJJJJJJIJJJJJJJJJJJHHHHHFDFDFCCB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:16987:87976 147 chrV 6130 60 43S57M = 5959 -228 CACTTCGCGAGCTTGCCCCCTATGCGTCACGCTATGGTCCCGCATATCTTCGCGAACGATTCGGAAGCCATGATTCCCAACTTGAGCGTATGCTTTATGG ###########################################D@;'/FFF;-(/8(0)B:):9:)8:??*1?DG@C<A<:IEE?@;DBAC?DDDD?@?8 PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:12756:79582 165 chrV 7559 0 * = 7559 0 GATGAGGAACGAGCCGTCGCGTCCTTGGGAACTTCATATCTGGAAATCATTTACAAAGATAGCTTATAATAAAATTGAAAAATTTCCTCATAATAACAGT :+1++2+++<)<0B<C2@D)1:0889B3(8?##################################################################### PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:12756:79582 89 chrV 7559 60 100M = 7559 0 GTGATTGTACAGCGACCAGAAGTCCTAGACAATGTCGAAAAGTGAAACCAACTGGAAAAAAACGATAAAAGGCGTATTTAAAAATCTTCTTCCTTCAGAC ########################################################?);?00:*?*DC:):)FA,CBA3A:C<<A2;:<2+B?;A7B?:? PG:Z:SNAP RG:Z:000 NM:i:6 +EAGLE:2:47:13418:12913/1 16 chrV 8644 60 101M * 0 0 CGTCGTCGTCAGGAATTCATGAGCCGTCCCGGTGTCTCGAGCTCTTGTGAGCATACACACAGACACATACAGCAGCAGCCTCCACAACATGCACATATAAA A=6=857::?9AAAAB?????8@=:>5A:?BADADA???5@DA:EEBEFBFBBBACAEA?EEBCBBBABEEFGBGGGFGGGFFEFFDBGGGGFDGFGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:47:2986:6932/1 16 chrV 10038 60 101M * 0 0 GAAGATCTAATAGCTTTGTAAAATTGATAATTTATCGTCAAAAATTTGACTTGAAACAGTCTAAATCGTGCAAAATACATACTTTTCTATTGTTTTATCAA EEEEEEEEEEEBBFEBGEEEGEFF?EEGEEGGGGEGGGEGFEEE?EFFCFFFF=FCGFGGGGFGG?GGGGGECCEDEGGFDGDGFFGDGEFGGGGGFFGGF PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:104:13973:5102/1 0 chrV 10071 60 101M * 0 0 ATTATCAAAAATTTGACTTGAAACAGTCTAAATCGTGAAAAATACATACTTTTCTATTGTTTTATCAATTATTTGAACACTTTAAAATTTAATTTAGCTAA FGGGGGGGGGGGEGGGGGEGGFGGG=EFFFEGGFGGGGBGGGGAGGFFGGGGFGGGGGGEGGGGFGFGGGGGGG?DDGEGGBGFGGFFGAGFGGGGFFGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:113:10088:6922/1 0 chrV 10199 60 101M * 0 0 CAAAATGCTGCTGGTCTGGCAAGAATAGGGCTGCAAGACTATTACCGTATTTCCTCTATTAGTCTTGCATGCAATACTAACAGAGGCTGCAAGACTAATTT GGGGFGGGGGGGGGEGGGGGGFFGGGFGGFGGGFDFGEGEGDGGGFFDBFGEGGEE=E?EFGBGBEEEEDFCEEEEEEBDGEE?EEE@ED:@B59ADDACD PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242 99 chrV 11315 60 100M = 11637 422 GTCTACGGGAGGGGGATCCTCTTGTGTAATACGTTATTAAATTATGCCTGTCAGAGCAGCAACACACACAGACAAGTGAGTCGTCGACTCAAAAAAAGAA CCCFFFFFHHFHHJJGIHIGIJIICHGGIIJJGEHIIJGGIDHIJGIIGIHHHCEHFFFFFEEEDDDDDDBDBDCACDCDACBBDDDDDBDDDD8>>5?? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242 147 chrV 11637 60 100M = 11315 -422 ATGCGGGAGGAAATCGTAGACATCTCAACTGATTTCGCATGGTTAAGAGTGTGCTGACGTCACAATTTTTCTGGGAAAAAATTCCGCATTTTTGTAGATC @DD@BCDDEDDB?=BDEDDDDDDDDCDEDDDDDDFFDDGGHHHHJJJIGHIJJJJIIGGJJGIJJJJIIJJJJIJJJJJJJHJJJJJHHHHHFFFFF@@C PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825 73 chrV 11689 60 36M1I5M1I17M40S = 11689 0 GGTGACGTTACAATTTTTCTGGGAAAAAATTCCCCAATTTTTTGCAGATGAAACCGCAATGAGACTTTCTGATAAAACGTCAGTACTCCACACGGCCGAA ++:+AD7D<+A,++AFE:F:,+++2++<:DB4:?)))0*000?'=8=/CFC;@@6@:A6B######################################## PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825 133 chrV 11689 0 * = 11689 0 TTTCTCAAAAATGTCACGTCAGCACGTTCTTCATAATGAAAAATCAATTGAGAATCCCGCATTTGTTTGTAGATCTCCGTAGATCAAACCGAAATCGGGC ################################@@DAA>D?@??;)==.)==.7E@8FD@FFFB>BBB::0?01))1)?CF<94BE?E<<FDFDD=?D@@@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:14381:50677 99 chrV 12587 60 100M = 12708 221 CTCAACATCCTGAAATAAGTTTTTGTCAGTTTGGTCTCCATTTTTTTGTCTGCAAACGATAAAATCTAAAGATCCAAAAATAAAATTAGTTAGTTTTAAC CCCFFFFFHHHHHJJJIJJHHIJJGGIIJHIJJIFHIJJIIJJJJJIJIJJJGIIIGIJ?EEFHGGEFFFFFDCC@CEDDDDDDDEDDDDDED>CDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:14381:50677 147 chrV 12708 60 100M = 12587 -221 TTAAAAAAAACAATGTGACTTGACATCACCGTCTGTGTTCGTCTAATGAAAAACAGCAGACCGAAAAAAAAACGGGTCTGTTTTAATTAAAAACTGATAG :1DB@DCC>@>AADCCA@CC>:>>>22&902?A<?@B@@DAC>:E@;;D@FEEHE>@GEDF;;JJIIGGGGEIJJJIJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:100:2124:19174/1 0 chrV 13308 60 101M * 0 0 TTTGATTTTCCTCTAGAATGTTGCTTCCGTCTCCTCAATCCATTTTCATTCCACATAGATTCTAAAACAGAAAAAAATCGATTCAGAAAAAAGTTAACAAA ?EEDEAFFFFFFGEGGAGDGGGGFGGGGGDEGGGDGGGDDE5EDEEFEAFGGGDDB?GGFGFGDDGGFGA?GGBFCDAEAGEEFBAED?EA>A=ADDAEEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038 73 chrV 14262 60 100M = 14262 0 ATATTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAATTGTGGGAATTTATGTTATCAACCATGAT CCCFFFFFHHHHHJJJJJJJJIJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJHIJJJEHHHHFFFFEDEEEEEABDBDDDDDFDFEEECDDDDDDDDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038 133 chrV 14262 0 * = 14262 0 TTCATTTGACAATACTCACAAAATTTTTTTGCCGTCCGAAGTGCCCTAACTCGGAGCCAAATTTTTCAGGCATTTTTCAGATCTCGCTTCTTTTACGCTT DDDDDDEEEDEDC@DDDDDDDDDDDDDDDDDDDDFFFHHHHJJJJJIIGFIJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJIIHJJJHHHHHFDDDFCC@ PG:Z:SNAP RG:Z:000 +EAGLE:1:73:5284:8999/1 0 chrV 14265 60 101M * 0 0 TTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTT GGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGEGCGGGGGGGGGGFFDGGFGGGDGEDBGGGGGEGGGGCGGGGGGEBFFGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:83:17647:3047/1 0 chrV 14268 60 101M * 0 0 GGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAT =E?DEDE?EDBDDD=DBDBDAAAACDFFFFFDEFF?CDD@DE=?BAA??A=EEEE?FFFF@EFFDBFBEFFEDF=BE?BE=@C?CBE@EEEEBE@EEADDD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:24:16337:8220/1 16 chrV 14278 60 101M * 0 0 TAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTGTGGGAATTTATGTTATCAACCATGATCTTTCATTTGACAATAG =DD@ECEBCEC?DFGGFFEFFEE5DEFEDDDGEGGGDGBGGGFFGGEGFGFGGGEGAGGGGEEEE?DGGGGFGGFGGGGGGGGDGGGAGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:7:10444:9975/1 16 chrV 14287 60 101M * 0 0 GGTCAAAAATCATAACTTTGTTCGCTGCTTATCGATTTTTATGAAACTGTATGAATTTACGTTATCAATCATGATCTTTCATTTGACAATAGTCACAAAAT BBEEDEEBEEDBEE:E=EEEEAD:BD?EEAEEEBABEEDBDDDEA?EEDEADBDBDDEBEBEE?EEE==EECA=;=DDB?AEBC=EDDD=DE:EBEDE=AE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:59:15165:16923/1 0 chrV 14304 60 88M1I12M * 0 0 TTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACTATGATCTTTCATTTGACAATAGTCACAACATTTTTTGACCACCCCAAG EGFGGEGDEDFFFBFGGAGGFGGGG@FFEFGGGAGGEFDGGBGGGG=GEFGFGCGAGFEGGFBGGCEEEE?C@BBAFEEFFFEBFFGDB?=FEDEGEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:42:11999:2677/1 0 chrV 14317 60 75M1I25M * 0 0 ATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAATTGACAATAGTCACAACATTTTTTGACCACCCCAAGTGTCCTAACTCGG GBGFDGGGDFAECFEFFGGFFEFDFEFF?EGAFGGGGGGG=EBBBEFFFFABCC=EDFEDEE@EBEFEEDEEEDGEB:?EEEEB=8BB:E5AA@:@@AAAD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:11:9361:9333/1 16 chrV 14441 60 53M1D48M * 0 0 CACGGCAAATTCGGCAAATTTGCCGTGCTTAACAAACTCCAAAAAATGTGACATTTTTTGATGTTTTTTGGAGCACCAAAACTACTGAGTTCTGAACAAAA =CEEDBGFGEBGEEFGEGDEEGGGGDGGGDFEGGGEGFGGGGGGGGGGGGGGGGGE=FGGGGGGFGDGGGGEGAEFGGGGGAGGGGGGGGGGGGFGGGG?F PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:82:11123:16509/1 16 chrV 14444 60 53M1D13M1I34M * 0 0 GGCAAATTCGGCAAATTTACCGTGCTTAACAAACTCCGAAAAATTTGATACTTTTTGATGTTTTTTTGGAGCACCAAAACTACTAAATTCTGAACAAAAAG BCBCACB=EEEAFGFEDFDFGGFFGFFF?FFEBEEADGGDGGGGGFGGGEGBDDGGGF@GDC@C@@5EFFFGFEEGDFFFFEFDFF=EEEBCCCEDGFGDD PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:120:10149:2029/1 16 chrV 17145 60 101M * 0 0 TTTTTTTTGTTGTTTTTTTGTTTTGCGAAAAACTTTTGAAAGTGTGATCCCACGTTCTTTTCTTGTCTGCGTCTCTTTTAAATTTGGGTTCTCTCGATAAA <C<8;AD?ACCC?ACA:AEGFEGDFFBFFFFBEEAAEDDDBGGGEGBFFEFCEAB?FDFGFGGGFAFGDGGGFGFGGGDDDE?FGGGEEFDFFFFFBCAEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:3:16562:7761/1 0 chrV 18570 60 101M * 0 0 ATTTTCAACTCCGGCAAATTGCCGATTTGCCGTTTGCCGGAAATTTTTAGAGAAATTTTTATAAGACGGAAGCATTTAAAGCTATATCATTCGGAAGAATT GGFGGGGGGEGGGGGGGFGGGGGGGGGGGGGGGGGGGGFGFGGGGGGGGEBGEGGGGGGGGGF=EEFGGFGGFEGGGGFGGGGEDFGEEGEFGFEBGDDFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:88:4071:9423/1 0 chrV 19829 60 101M * 0 0 ATTGAGTTTTTCTCCCCATAAAAGAAGCGTTGACTAGGAAAAATGAATGCAATTTTTTAGTTAGCAGGTACACATTATTATTCAAAAGTATTTAGAAGTTT EFFFFBBDEECDB>?DDCDDADEE?CCED:DD5DDEF?FE?DEAEFFF:F??:DDFDFDFFF==DCBEEEB?EDEFD=DEFBFDFFFF?FFAFDFD:EB5C PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:42:18060:2997/1 16 chrV 23388 60 101M * 0 0 TTTTTCCGATTTAATTTTTGATAGGTTTTCTCCGCAAATTTTCCACTTCAGTGCATGGCACACAAACGGCATGTTATAGTAATTTTCCAATTTTCCAAAAG FDDECFADDB@FEEGED?EGGGFBEGFG?GEGGFGGFFGFGGGDCGGCGGEFGGDGGGFEGDFFFDGGGGEGGGFFGGGGDGGGGGGGGFGGEGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:13:6904:13626/1 0 chrV 24494 60 101M * 0 0 GGTTTTTCTTGAAATATCGTTTTTAAGGTCAATTGATATGATAATAAAATATATTGTTGACCTTAAAACACATTAAAAAAACTTCGACAAGAACCGACAAA EDEDABDA8BCF=FF=EEEDFFDFFFDA=FFEDEFBFADF:EDEED=FEEEFFD?DAEEB=DEDEFEDFCEABEEFFE?B8CDBFAFDFFFEFEBFEFEDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:47:11060:17697/1 0 chrV 24606 60 101M * 0 0 CGGTTGAAAACGATAAAGATAAAACATTTCGTAAATATACACAAATCTCGTAAATCGACACATGGCGTTTTTGGCGCGAAAATTCGGCGTTTGAAAAACTT GGGEGGGFAGGGGADGFDDGGGGGGGGGFGGEGEGDGGGGGGDFDGGGFEDGGGFGEFGGGGBF=EE@BCFEFFDFEFEEEEEEGEBAFB=E==BFEDE@B PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807 99 chrV 24799 60 100M = 24916 217 AAGAGTACCTAACCAAAGAGCGACTCATCAATTTTCTGAATGAAGAACAACGTGATCCACGACTCAACGAGATCCTCTTCCCATTTTTTGATTCTCAGCG @BCFFDDFFHGHHJJJJJJIJJIIGIGGGHGIJJJJJIIJJJIJJJJJIJJICGGGHGIEHGFHFFFFFDC>BDDCDC@CCCCCDEDDDDBCDDDDDD<@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807 147 chrV 24916 60 100M = 24799 -217 AAAAACACGAAAACGACATAAAATATCAAGAAGATGGAAAAATGTCCGGTGACGGTTTTCTCCGATTCCTGATGTCAGACGAGAACCCGCCAGTGTTTCT ?CC?8<<-:BB<?AABA>4DC@::A@@CCAC>:44>(DDDC@5=;??@;;;;8?;)B6/:55()@=)@8)==.))6DB6?990B?JJHHGHHFFFFF@C@ PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:45:11954:18961/1 0 chrX 1563 60 76M2I23M * 0 0 TCACAAAGTTGCAGCTCCGAAAAGAGAATCCGTCTGGAATTCTGGAAGTTCACAAGAATTGTGAGTACTTACCTTTTTCAAGTGAAACTAGAGGAAAAATT GGGGGGGGGGGFGGGGGGGGGGGGFGGGGGG=GGFGGFFGGGGFGEDFBDGFGGGGAGGGFCFEFCDDEEGGGFGFGGEAFFBFCEEFEDDCDGAADEBDB PG:Z:SNAP RG:Z:266-1 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667 99 chrX 5712 60 100M = 5891 279 AATAAAGTCCTGCCGCGAATGCTTCAAGGTTACTGTAGTTTTTGGTGGTGAGACCCGGAAATTTCTTTATTTTTCAAAAATTTCAGAACCCGGTCATGAT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJGIJJJJHIJIIJJJJIHIJAGIJIHHHFFBDDDDDDEDDDDDEEDDCDDDDDDDDECDDDDDDDBBDDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667 147 chrX 5891 60 100M = 5712 -279 TCTTGGTGGGTCCCGTCACGAATTTTTGGAAATTATAGTAAAACGCCAATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGA DBBDDDDDDDB>DDFFFFHHHHJJIGGJJJJJIJJIJIJJJIHHJJIJJIJJJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118 99 chrX 5939 60 100M = 6055 216 ATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGATTCTGAAATTAATTTTTTACTGAATTTGGCGAAAAATTGCAAATTTTT CCCFFFFFGHHHHJJJIIGGIGIIJJCHHJII@HHIIJIGJJHIIJGICB?GGHIIJGHHJBGEIFIHHJIJJJIJGIJ;@CHD<><?ACCDCACC>@@A PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118 147 chrX 6055 60 1M1I3M1D95M = 5939 -216 AACCTGGTGGTTTTCGTGGCGGGACCCGGAAATTTCTGAATTTACGCAAACCTGGTCATGATTGTACTTAAATGAAAGCTCCTAGCGAACTGAAATCGAA @CBA@BDDBDDBBB=BDBBBAAADDDDHHGHEGIGIJIIIJIGGGEHHF?IHJIIIJJJJIJJIIIHHDIIIHIIIGJIIJJJJIIHFFHGHFFDFDCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752 99 chrX 6879 60 100M = 7127 348 TTTTCAAATTTTTTTGGAAGTTTTCCAAAAAATCTCTGTTTTTCCCGCCGCTCAAAACCCTCAATTTTTCAGATTTCCCTGCTGGAAATGCTCGATTGCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJJJJJIJJJJJIJIJJJJEFFDEEEEDDDDDDDEDDDDDDCDDDDEDDDDDDDDACCACCCBBDDDA: PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:8575:6989/1 0 chrX 6976 60 101M * 0 0 GCATTGAAATTATCACTGAAGAATTCAAATCTCCAAAAATGACGTCTCCGTTCTCATCGCCATCTACCCGACAATCAATCACTTATATTCAAGCGAAAATC GGGGGGFFGFGGGGGGGGGGGGGGGGGFCGGGGGGFBGFGGFGGEGEFGFEGGEGGGGEGEEEGEEEGGECD@CCFCD@FAECC@CAACECDBCDA?CCCB PG:Z:SNAP RG:Z:266-1 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752 147 chrX 7127 60 100M = 6879 -348 CACAATGTCATCGAAACCACTCTCTTAAAACTGCAAATTTCTGGATTTTCGACCTTAAAATTTTTAAAAATTCCCAAAAAATCAAATTTCTGTCAAAATT CEDDDDDDDDDDDDDCDCCDEEEEDEFFFDFFFHHHHHHJJJJJJIJIIHGIJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:118:7183:17666/1 16 chrX 7624 60 101M * 0 0 AAACTTTCTTGGGTTATGAGTCTCAACGTATCCACGTGCATGACGGAAGAATTGGCTCAAATGCTCGCGAAGCTGCTCGGCAGACGTGTAAACGTCCAAAA 9=:?7@?C@CACA?ECCC=CCC:B?5B@BA:A=CCEACDDBEEFFGFBBGGEGGDDGGGGF=EGFGFFGGGGGGEGGBEGGGGGGGGGGDGGGGFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:55:11225:9062/1 16 chrX 7903 60 101M * 0 0 CAAAAAATGAAAATTTGGGCATAGCTTCCGCATCTCCAGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATGTTTCAG EGEEEEEEEEEADBBDGGFEEEDBEBCEFDGEEGEGGGFAFEEGGGFGGGEGFGGEFGGEGFEEFGFGGGGAGGGGGFGGGGFGGGEGGFGGGDGFDGFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:78:6338:2856/1 0 chrX 7939 60 101M * 0 0 CGGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTTGGATA DDD@DEEEEEGBDEGFGGFGGGGEGGFGEGGEBFEBEFFADDEFFFEEEEEDEEEEEDEEEEGBEEEEEEEEEEEEEEAEEEEEEEEE@EEEEAECCCABE PG:Z:SNAP RG:Z:266-1 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162 73 chrX 7942 60 100M = 7942 0 TGACAAGTGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTCGGATAGA @@@FDDDDFDDDAGFHJJGIDHICFHEFCBFGGBDA@BDDGEH:?GA>GEFG<?DGHDFGGGGEB<BHHHEEC>DCDEEEFDEE??>CA@A@C@B@DBA> PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162 133 chrX 7942 0 * = 7942 0 TAATAAGCCACTTTTCAAATAATCGCTGAATTCTAAGTAAAACATGTTTATTTTCAGTATATTTATCAGATTTAAAAAAAAAAGTGAGATTGTGTTAGTT ##########A:3CC;<DC?A?B@CCDCDCC@;C>AB;)7)))3=.7.))=D=.))));=;=)8B<FB89FB(GDHFA@HCA2E4?A+2+3FA+2A+1+1 PG:Z:SNAP RG:Z:000 +EAGLE:1:50:11162:12440/1 16 chrX 7960 60 101M * 0 0 GCACAAATTGTCGGTTGGAATACGGTATTTATTGAATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTCGGATAGAGAAACACACAAGTCACAGA FAEEGGDGFFEGGGGGGGGGGEGGDGDGGFGGGFGEGFGGGEFFAFGGGGFGEGFEDFFFCBGGEFGFGEGEGGFGGGGGGGGGGGFGFGGGGGEGGGEGE PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:103:14619:17650/1 0 chrX 8476 60 101M * 0 0 ATTGTTTAATTTTTCCGGAAAAAAACTGAAAAATCCAAAATTTTTGAATTTTCTTCATAAAAAGTCGGGAAAACCCAGAAATTTTCCAATTTTTTCCGGAA GGGGGGGGBGGGGEGGGEGGFGGFGGGGDGGGGFGGGGEGGFGGGFGGGGGGGGGGGGG?FACEDGGEGDDGBGGGFFFGCFEGGGGGBGGGGGGE?DGEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:5:12119:5229/1 0 chrX 8820 60 101M * 0 0 TTTCTGAAATCCATAATTTTTCCAATTTTTTTGGGAAAAAATCCAGTAAAAGCCAGTTTTTCGCAAATTTCCATCCAATTTTCACCCTTAACGGCACAACA EG?EGGAFGGGDGDDAGGGDFFFEGGGGFGGEGEEGEGGEGDEGGGFGDFFFFDGGFGAGFDG?GEB?FFGFGGGDDEGEEAFDF5DECE:CE=E=CCECD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:73:11201:4957/1 0 chrX 10521 60 1D6M1I94M * 0 0 GTTTTCCGGGAAAACTGAAAATTTGGTCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATGCGGTATTTATTCAC GGGGEGGFGEBCECEGGDGGGGDGGGGGGDGGEFGEGGGDGEGGGFFFFFDFFFFGEGFFFGFGGGGEFGBC?DDFFGDFFFAFFEEAEE?ADE=DEEEDE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:46:5340:5404/1 0 chrX 10534 60 101M * 0 0 AAAGAAAATTTGGGCATAGCTTCCGCATGTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGCCAGAAATACGGTATTTATTCACATTTTCAGCCCAA GGFGGDFGDG?F=FFGEEGGEGGGGBCDGEGGEEFEFCFDEEEEBGFGEGGFEFEGGBEFFF@DEFFFDD??BBC???@?EEEEEBBBBBEEEE?EEEEEB PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:10:12798:8231/1 0 chrX 10540 60 90M1D11M * 0 0 AATTTGGGCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTGTTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTAAGCCAATTTAAGG GGGGGFFFFFGGGGGGGGGGGGGGGGGFGEEGGGGFGGEGGFGGGGGGGGGGGGGGGGGGGGGEE?EEEEGEGGGGGGFGGGDGGEDGBAEGGEFDGEAGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:113:16640:19734/1 0 chrX 10540 60 90M1D11M * 0 0 ATTTTGGGCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCCATTGCCAAATTGTTGGCAGAAATACGGTATTTATTCCCATTTTCAGCCAATTTAAGG FGGGGGGFGAFDFFFGGGGGDGGGGGGGGDFEEDBFFFEFGGGGFGGFGGBBGGGGGGGGFBGEEGFFECF??EECCEECEFEEEEE>CB:@BAACEEDEF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:75:12666:4476/1 0 chrX 10543 60 87M1D12M1I1M * 0 0 TTGGGCATAGCTTCCGCATGTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTG GGGFDGFFFGGGGGGGGGGFDGGGGGBGGGFFFFFFFDGGGGGGFGGFGFGEDGGAEGGGDGGGGGFFEEFFFEDGGEGGGGGGGDFGGEBDFG=EEFFCB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:56:15981:20326/1 0 chrX 10549 60 81M1D20M * 0 0 ATAGGTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTGAAAATG FFFFFEGGGEFGGGGGGGCEBGGGGGDFEDGFFEGFGFGEEEEEGGGEEEBFAEADDBDBEE>EEEEBEE?:???ABA??AAA@AABB?B88A>?BBBB<A PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:26:17260:3333/1 16 chrX 10550 60 101M * 0 0 TAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGGCCAATTTAAGACTGAAAATG BB=BB?=B=BB:B=BDCBB:B?BABBCD?BDBAC=BBDCAAA:AD:DCBDBB@?BDA>C:C;C?@;:C?:@CCAC?CA>AA=5BB;:5A?:D=?DDDD5?D PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154 99 chrX 11838 60 100M = 12309 571 GTCTCCAGCTTCAAAATGACCCCCATCATGCCCTGTTTCCGTGCAATTTGAAAAATTCGGGTCTCGCCACGCGTTTTTTTTTGCAATTTTCGAACGAACT CCCFFFFFHHGHHJJIJJJJJJJJJJJIIIIJJIJFHIJJIIIIHIIIJJBGGGJJJJFIIEHGEFHFFFDDDD6;@BDBDDDDECDDDDCBBBDDDDBA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154 147 chrX 12309 60 100M = 11838 -571 TCAAAAATATTGAAAAGCCACCGATAGAGAGGATTTAGTGTGAAGAAAAAGGGGTGAAAAAACACCGGAATTGGGAGAGATATAGGACACGTCCATATCT 05CDEECD@C:5C>??@BD?@DCCEEEEDDFFCDFHHHHHED@7)JJJIIJJIJIJJIHGIGHGIHGJIJIJJJIGIIIJIGIJIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:20052:62684 99 chrX 12814 60 94M6S = 12857 139 CTGTATAACAGTGTGTAATTTCAAAAAAATCGGACGCACACACGCTGACTTGTGAGGCTTCAAAAAAAAAGTTGAAATAGTCAAAAAAAAAATCCATCAT CCCFFFFFHHHFHHHIHIJJJJJJJJJJJHGHJIJJJJJJJJIHIHHHFEFFFFDEECDDDDDDDDDDDB>:CCC@CACC:CCDDDDDDDDD<A###### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:20052:62684 147 chrX 12857 60 4S96M = 12814 -139 CCACGCTGACTTGTGAGGCTTCAAAAAAAAAGTTGAAATAGTCAAAAAAAAAATCCATCATTTTTCTGCCCAAAAATTGAAAAAACGGGCACCTATGCAC ####CC@::<DCDCCCA>>:8-BDDDDDDDDCCA>DDC:C><=DDFHHGFG@IGGJJJJJJJGHEIGIIHBIJJJJIIIGJJIHGIIHHHFHFFFFDB@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:61:3791:13082/1 0 chrX 13027 60 101M * 0 0 AAAATATGAAAATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATT GGGGGEFE?GEEDBEEEEEEFGFGGEFFEADGGGGE?GFFGGFGGGDGFGFFGFFFFBGGBFEEFGGGFGGDGEEBGEEEFFGDBEBGFEGFEEEGEEBGD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378 99 chrX 13038 60 100M = 13177 239 ATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATTCAAAACACTA CCCDFFFFHHHHHJJJJIJJJIJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJI7FHJJJIJJHHHHHHHFFFFFFEEEEEDDDDDDDDEEEDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378 147 chrX 13177 60 100M = 13038 -239 TATTTCTCAAAAAAGATTATCTTAAAATGTTGAAAATGATTATCTTAAAGATTATCTTAAAATGTTGAAAAAAAAAACACCGACTACCGTAACCCAGAAC @DCCBB?BDDDDDDDDDDDCDCDDDDCDDDDEEFDCCCDDDDDEEDEEECEFFFEFFHHHHEAGIGGJJJJJJJJGGGFJIIIIHGJHHHHFFFFFFBBB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:84:6328:7727/1 0 chrX 15454 60 49M1I37M2I12M * 0 0 CAGTAACCTGACGCTGAATTTCGCGGCGAGACCCAAAAATTCCGAAAAAACCATCAAAAACAATCAAAATCTGCCAAGAAGGAAGGGGGTTTGATTTAAGT GFGFGGGGGGEGGGGGGFDFGGGGBGGFFBEGEGBGGGFBBGFEDGFGBG?GGEFFGGGFGEE7EBDEFDDDECFEECEE@EACCCCCC6:AA@:AC?C@; PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956 99 chrX 21169 60 100M = 21409 340 GTATAATCATGACTAGGTTCCGGTAATTTCGGAAAATATTGGGTCCCGAAGCGAAATTCCCCGGGTTACTGTAGGCCTTCGCGGCGAGACCCAAAAATTT @?@FFFFFHHHGHJIJJCFIIJFIJIGIJIIGDGGIFIIJGIJDFHIIBG>HEDGHFHFFFFDDBBBDDDCCC>C>>CAC@BB9999>09@ABBDDA<:> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956 147 chrX 21409 60 100M = 21169 -340 ACCTAAAATTTCGCGAGAAATTCAATTTTCAAGCGAAAAATTTTAATGTCCGAACGCAGATCGTCGAGAGCTTTCATTTAAGTATAACCATGCCTTAGTT CCC@DC@B>@@BB?<ECCA@>>CCCADDDDDDBEEFCEAEECDJIIGHGGIHF?GEGJIIEIHD<EBGFGIIIHIJ@GGIJIIIGBAGGHHGFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:18264:40909 99 chrX 23955 60 100M = 24075 220 ACCGTAACCCGAAATGTTCAAAATTTCGCCGCGCCGGGGGTGAAAAATGACGGAAAAATTGAATTTCGCACTACTTTTTGCGTATTTCGATTGGATTTCG CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJHFD=@DDDDDDDDCBDDDDDDDDDDDDEDDDDDDDDDDDEDDDDDDBDDEEDDDDDDACDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:18264:40909 147 chrX 24075 60 100M = 23955 -220 TTCTTAATTGGCGGATATTCGACTTTGAGCGGATTTGAGGTTCTAACAATATCAGAGTCGGCGAGCGACAGCCGCGAGAAGTTGTTCCAGTCTTTGTGCA CCACDDDDDDDDCCEDDDDDCADDCDDDDDDDDDDCDDDDDDDDDEEFEEECEEDFFEGJJIJIJIIIJJJJJJIJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:62:3773:4884/1 0 chrX 24873 60 101M * 0 0 CCCGAAAATTTGAAAATGTCCGCTAGAGACCCGGTAAAAAGACGTTCTAATGTGAAATTTGGCGAGGAATTCGAATTTCATATTAAAATATGTTAACATCA GGGGGGGGGG?FFEFEGGFGGGGFGF?DFFFFFFCGGGFGGEGFEAEEBEDFCEBEEDDE5BEECCEACCCDECAEEEEEECDEEBEDCCACACCEEE@EE PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:111:17901:21150/1 16 MtDNA 148 60 101M * 0 0 TAGGATTATTAGATATATTAATATTGACCCTATAAAAAGAAGTTTTTTTCTTATCTTTTCACTACTTTTTAGTATACCAGTTATTTCAATGAGAATACATA CB???CA=AC=BBCADBDC?B?B?BA5DCAA8C?ADDABDC?CAACDDDDBDDDDDDADDBD=5DDDDBDDCDDDBDDDDDDDBDD=DDBD:DDDB>AACC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:24:11474:6931/1 16 MtDNA 422 60 101M * 0 0 GGTTTAAGAGGTTTTTATTATAGTATTTACTGGTTTATTTTTTGTTTTATTTTAGTATGTTTATTATTTTTTATAAATTTTAGTAGTTATTTTTTACATTT DCCCA5:DDBC>BCD5D:DD=:D6DDDDBDD?CCCCACCCCCDC<CC>B=DDDBDDCDDDDBCCCC<CBDBDADDDD=::D=EED@CBBBE:DBDD5CC@A PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1215:12199:50114 99 MtDNA 542 60 100M = 651 209 GTTTAAAATTATGTTTTTATTTGTTAGATTATTTATATTTATTTTTAAATGACAACGTTTAATTTTTATTCTAATTTCTTTAGAATTTATAATGTTGAGA @@CFFFFFHHHHHJJJJJJJJJJJJJJGIJJJJJJJIIJJIIJJJJJIIJJIIIHIIIJJJIIJIJIJJJHJJIJHHGIJJJIDFHHGFHFDEFFFFEC= PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:37:4190:5792/1 0 MtDNA 597 60 101M * 0 0 CGTTTAATTTTTATTCTAATTTCTTTAGAATTTATAATGTTGAGATTATTTTTAAAATTTTCTTATGTTTTAGGGGAAATAATGTTTTTTTATTTTATGTG GDEGGGGGFGGGEFGGFGFGGFGGGEGEGGGGGGGDFGGFGGEFGGGEFGGGGGGGGGGEGGGEEFGEGGGGGGGGEEEGEDGG?EEEAAE7EEEC?EFAA PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1215:12199:50114 147 MtDNA 651 60 100M = 542 -209 AAATTTTCTTATGTTTTAGGGGAAATAATGTTTTTTTATTTTATGTGTTTTTCTGTTATTTCAAGAATCCTGGGTATGGTAGTTATAGTAGGTAATATAA ADCCDDDCDDDDDDDDDDDDEEDFDEFFFFHJJJIJJIJJJJIJJJJIIHJIIIJIIGIGDIJJIJIGIJJJJJJIJJJJIJJJIJJHHHHFFDDFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:51:1652:7346/1 16 MtDNA 726 60 101M * 0 0 ATGGTAGTTATAGTAGGTAATATAAAATTTTTTGGTAGTGATAATTGTATTTTTTAGTAACAGATATAAGTTAAGTTTAAACTATTGATCTTCAAAATCAA BEEFEFEEEEBEFBFEEEAFEFFEFFAFFF?EFFFFFFEFFFFFFFFFFAFFFFBFFEFFEFFFBFFEFFEDEDFDFAFFFFFFFFF?FFEFFD?FEFFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2207:3477:80131 99 MtDNA 800 60 100M = 937 237 GTTTAAACTATTGATCTTCAAAATCAAAAATTTATTTCTGTAGAGATAATAGTATAAATAAGTATGTTTCTTTTTCGCAGAAATGGTTTTTTATCTTATA CBCFFFFFHHHHHJJJJJJJJJIIJJJJJIJJJIJJJJJJGIJJJJJJJJJJIJJJJJJJJJHFHIGIJJIJJJJJIIIIIHHHHHFFFFDDDDDDDDCD PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:16898:20473/1 0 MtDNA 800 60 101M * 0 0 GTTTAAACTATTGATCTTCAAAATCAAAAATTTATTTCTGTAGAGATAATAGTATAAATAAGTATGTTTCTTTTTCGCAGAAATGGTTTTTTATCTTATAA ECEE?FFFFFGGGGGGGGGGFGGGGGGGGGGGGFGGGFGGFFGEFEGFEGEGFDGEEFGEEGEEEEEEFEFGEFEEEEBG?ACDEFBDDEEDCB=FEEFEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2207:3477:80131 147 MtDNA 937 60 100M = 800 -237 TAAGATGATTTAAAATGTTATTATCTAAATTTGATTTACAGAGTAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAAT DDDDDCCECDFEEDEEEEEEFFFFFFFHHHHHHJJJJJJJJJJJIIIHIGJJJJJJIHF?IJJJJJIJJJJJJJJIJJJJJJJJIGCHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:46:4245:14190/1 16 MtDNA 952 60 101M * 0 0 TGGTATTATCTAAATTTGATTTACAGAGTAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACTTGT CGBEEDEDEFEGEGEGEDDGFEEBDGGGDBGEE@EFGGGGGGFECEGGGGEBFFGGFGFGGGGGGFGGDGGEFFFFEBGGFGGFFEDFGGGFGFGEFGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:27:7941:4769/1 16 MtDNA 1010 60 101M * 0 0 TCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATCT CG?BGGGAFGFDDCFBGGGFFEGGGGDGGEFGEGFGGGGGGGFGFGGBEGGFFDADGGEGGGGGGDGGGGGGGGGFGGGFGGGGFGGGGFGGGGGFGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:84:2306:5557/1 16 MtDNA 1132 60 101M * 0 0 TTAGTGAATTTTCATAATTTTAAGATTTGTTGAACAAAGCAGATTAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAA CCE?EEACBBEBFFFBEEE5EABF?FFEFFFEDAE=EFFECEDEEDDD@DDEEEDDDECFFFFAFFDEBE?DFFDFFFFFDDEEDA=EEEED:DDDEEBEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:111:11302:7630/1 16 MtDNA 1137 60 101M * 0 0 GAATTTTCATAATTTTAAGATTTGTTGAACAAAGCAGATTAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAAAGAT AEEAEE:EAECD?BB?DGGGAGDFAGGDDGGGGGGGFF:FFGGDGGEEEDEAGGEGGGFGDGEFDGGAFGFFGFGGDEDGGGGDDGGAG=GGGGFGEGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:2947:30776 99 MtDNA 1208 60 100M = 1331 223 GGAGTAAAGTTGTATTTAAACTGAAAAGATATTGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTT BCCFDDFFHHHHHJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJIIIJJIJJJJJJJJJJJJJJJIJFFHHIIJJIHHIJHHHHFFFFFDEEDDEDEDEEE PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:43:15776:12730/1 16 MtDNA 1242 60 101M * 0 0 GCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTGACTCGTGTATGATCGTTTATTTTATTCTTAA EDDC:EDDCEBGEEEEDEEEEEGFGEEEGGGEFGGGGDGFGFFACEAGGDFFFECFGCGEGCEGGFEF?GDGGGFGGGGGGFFGGFGDFGGGGGEGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:2947:30776 147 MtDNA 1331 60 100M = 1208 -223 TTTTATTCTTAAGGATTATAATAAAAAATTTTTAATTTATTAAAATAGATATATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATAT @DDDDCDACDDEDEFEEEFFFFFHHJJJIJJJIJJJIIJJJJJJJJIIHDJIGD?JJIJJJJJJJJGJJJJJJJJJJJJJJJJJJIHHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1208:16741:52367 99 MtDNA 1375 60 100M = 1446 171 ATAGATATATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAAATTGTAAAAGACA CCCFFFFFHHHHHJIICHHJJJIJJJIIGIJJICHJJIJGJGIJJJJEHIJIHIJJIIIIIJJIIIJJFHIJJIDCEEEHHHHFFFFFFEEEEECECBDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:51:9770:4736/1 0 MtDNA 1428 60 101M * 0 0 TATTATGGATTTTAGTTTTAGTTAACTAAATGAAATTGTAAAAGACAGTAAAAAATTCTTAATGTATTTTTGAAGATTATCTAGAAGTGGTACAAATCATC FFEEFDFDFFFFFDFBD5DDEEEEEEEE=EEEEEEEEE?EEEEEEEEEEEEEEEBCEEEDEEDEEEEEEEEDEABEDDEEEEEDEEEBEEDEEEEEAEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1208:16741:52367 147 MtDNA 1446 60 100M = 1375 -171 TAGTTAACTAAATGAAATTGTAAAAGACAGTAAAAAATTCTTAATGTATTTTTGAAGATTATCTAGAAGTGGTACAAATCATCCATCAATTGCCCAAAGG DDDEEECEEEFEFFFFFFFHHH@FFHJJJJJJJIIJJJJIJJJJJJJIIIJJIJJJJJJJIJJIGIJJJJJIGGJIJJJJIIJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:21063:70183 99 MtDNA 1578 60 96M4S = 1595 108 ACCTGAATCTAGTAATAAAACTATTTTTAAATATGTTTTGAAAACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGTTTAATATAGAATTGTTGAT @CCFFFFFGHFGBGGGHJGFHIHGIJJICGHIEIHGIJGIFDDDGHIG@D?FEHHIHHHHIJGGB@CF@GC@DHE3.?EHHHCF7@37.6>6>>;@#### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:21063:70183 147 MtDNA 1595 60 9S91M = 1578 -108 CTAGTAATAAAACTATTTTTAAATATGTTTTGAAAACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGGTTAATATAGAATTGTTGACTGTTAATC #########@B>67;3@;B?<HC;==JJIHGGFFHF=89B@GGHFB8DBB?8)?*C<>JIIIGFC9A2?C<++JJJJIIEIHFC;EJHHHHHFFEDDBB@ PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:62:8987:8931/1 0 MtDNA 1620 60 101M * 0 0 AACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTA GGGGGGGGGGGGGGFGGGGGGGGGEFGGGGEGDGEFFFDFGGGGGGGGGGGGGEGFEGGGEFDFGGFFFEFFAFECEEEFEGEEGEGEEGBFBEEEDCDEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:58:15307:9170/1 0 MtDNA 1657 60 101M * 0 0 GTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGC DBDD:CDCBCEBAEEEC=EEEBCBBB=BBB@>>CC5C>AA?>>;>@@FBD@@E:B@@@BB@@5B?B>BBBAAA:A@BB@@@D@@@@@A?BA?@>@@@@@@? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:87:9699:18394/1 16 MtDNA 1707 60 101M * 0 0 AAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGCTCTTGATAATTTTAGTTTTACTTATAGTTATTTTAATGATGATTTTTATT BEFGEBEEGEEEEEEGBEGGEEFGEGGGGGGGGGDGFEGEGGGGGFDGGGGGGGDGGBGGBGGGGGFGGGEGGGEGGGGGGGGFGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:100:3634:12538/1 16 MtDNA 1809 60 101M * 0 0 TTCAAAGAATCGCTTTTATTACTCTATATGAGGGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAA EA=EE??ED:EA<?:A=EED5EEE=E<<>B>8;;9?EE?EEEEEDDDEAEEEBEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA?FGGGGDDGEGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529 99 MtDNA 1832 60 100M = 1878 -100 CTATATGAGCGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAC @B@FFFFFHHHHHJIIJFHIIJJHGIIHHGJJJJJIIHGHIIJJJBFHJIFGGFHEIHIIIHHACHHFEFFFEEEECEBBCDDCDDDCCDDD@DBB?CAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529 147 MtDNA 1878 60 46S53M1S = 1832 -100 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACCAANGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAN ##############################################IHGFF?0#IIGEIIJIIJJJJJIIIJJJIIJJJIHHHHIJJHHGHHFDFDD=1# PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203 99 MtDNA 1898 60 100M = 1981 183 GGATTAGCACAAGCTTTATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAA BCCFFFFFHHHHHJJJJIJJJJJJJJJJFHIJJJIIIJIIIJJIJJJJJJGIIJJJJJHHHFFFFFFEEEEEDEDDCDFEEEEEEDBDDDDDCEDDDDD< PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:73:15682:20928/1 0 MtDNA 1914 60 101M * 0 0 TATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATA GGGGGGGGGGGGEGGGGDGGGGFGGGEGEGEGGEGGFGFGGGGGBE?GFFEEGEGEECEC=CCCCEEEEECC@D?ACEC:CDACCEEEEEEE;EE6<AA?? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:8075:44880 99 MtDNA 1923 60 100M = 2060 237 GGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGA @@@DDDFFFHHFHGIIJJIJIGGIJJJGIIIIIDIIIDGEHIGEGGIECHHFHACFHIIIIJGIFFCEEHEFFE>;ACECCCACBCBDDDDDFDDEDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:64:1358:10984/1 0 MtDNA 1931 60 101M * 0 0 CTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTAC GGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGFGGGGEEGGEGGEEEGEGGEGGAEF@D@CDDE@FDFEDDDDE@EEC?@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325 99 MtDNA 1965 60 100M = 2135 270 CCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGA CCCFFFFFHFHHHIJJJJJJJJJJIIJJJJJJJJJJJIJJJJIJJIJJJJJJJJJJJJIJJIJJJJJJJJJJJJJIJIJIIHDDBECEDECDCDCDDDD< PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203 147 MtDNA 1981 60 100M = 1898 -183 TTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGAGTATTCAGTTTTATTT DDCCDDDDDCDDDDDCEEDDEFFDBHFHEHHHJJJJJJJIJJJJIHFGHFIIIHGJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:118:10385:6513/1 16 MtDNA 2048 60 101M * 0 0 GATTTTATTAGTTTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATA DEEFGGFGABGGEEFGEGGFGEGGGGGGGEGEAGGEFGGGGGDGGAGGGGGGGGGGGGGGGGFGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:8075:44880 147 MtDNA 2060 60 100M = 1923 -237 TTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATATGGTATAATTG >>CCDCEDCC@CCDDDCDDBDEGHHHHHGCGGHIGHGGIIHEIGIGHGIFHHIGGHIGIIH@GIIHGGGGHJIIIJJIIHEA4IIIIFHHHHFFFFF@CC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325 147 MtDNA 2135 60 100M = 1965 -270 AGAAAATCAAAATATGGTATAATTGGGGCCATCCGTGCTAGAAGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTC CCCEDDDDEEEDDCCEEEEECCBFFHHHHJJIIJJJIIJIJJJJIJJJJJJJJJJJJJJJJJJJIHGJJJJJJIJJJJJJJJJJJJJHHHHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:44:5556:17346/1 0 MtDNA 2177 60 101M * 0 0 AGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACT FFEGGGDFGGGGGGGGDGGGEGEGGGEDGFFGFGGFGFDGGGEGGEDFGGGGGG?GGGGEDEFGGGEGGGGGGGGGFFGEGGE?EGEFGGEEGGDFBBAGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:90:16871:20538/1 0 MtDNA 2198 60 101M * 0 0 ATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACTTTTAATTATTTACATCCCATT FGGGGGEGGGGFGGGGGGGGGGGGGGGGGGGGG?GFGGGGGGFGGGGGGGGFGGGGF?GGGGDGDGGGEGGEFGGEGEGGGGGACGGGGFGDGGGFGFGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:1:15979:20440/1 0 MtDNA 2341 60 101M * 0 0 TTTTTCTGAAGGTGAAAGGGAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTT GGGGGGGGEFGGEGEEFEEEFFFFFFFFDFFGGEGGGGDGGFGFEFEFFFGGDGDGCEGFGDGGGEEGGEGG=FGGEG?AGGG?DAEBEE=CFAEDDDDEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387 99 MtDNA 2360 60 100M = 2687 427 GAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTT B@BDFDEEHGHHHIFHIJIIIJBHHFHGFHIJJIIFHCBHGIJJJJGIJJHHJIJJJJJIEIJIEBGHIJJHHGEGHIEGGHGHDDDBCDD>CDDEEDC@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:86:11924:13707/1 0 MtDNA 2394 60 101M * 0 0 GAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCA GGFGGGGFGEFGDFGGFGGEGGGGGGFGGGEDFFGFDDEFGGGFFGGGGGGGGEGDFAFFFGGGGGGEGGEDFDDGGGGGEEEEGFGGEEEGEFGDC:=F5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:23:12390:3932/1 16 MtDNA 2447 60 101M * 0 0 AGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCATTTAGTATTTTTTCATTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCG @A?A?:EE:?E==FGBDGEEGFFFB?DFBDEGG:FD>EGGBFGGFEFFGGEG@GEEFFE:BGFDFG?GDFGEGGGEEFFFEEF?FFEE@BDDFDFFDFDFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:69:4343:18981/1 0 MtDNA 2510 60 101M * 0 0 TTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCGTTATGATTTAATAATAAGTTTATTTTGATTTAAACTTTTACCAATCTCTTTAATTATATTGTG EECCCDDEEEFFEEECDDADEEEEDFGGFFGGGGAGGGGFEEE:EEEEEEF?GDDGEGGGGGGDGBFGDFFEGGGFGBGCDDFDFGEDGGAGGAGBFEBE? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:106:17241:4836/1 0 MtDNA 2631 60 101M * 0 0 ATTATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAA GGGGGGEEFGGGGGGGDGGGGGGGEGGGGGGGGAFGGFGEGDGGGFFGGFFEFGGGGGGDGGFFGGGGGFDGG?ACEEFFGDGEDFDD=FBAEEEFBF?FF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:118:1597:14954/1 16 MtDNA 2633 60 101M * 0 0 TATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAAT FGGGEEGGBECG?GEGGGGGFGGBGEGGBFGGGEFGGFFGEAEDAEBGDGGGEGFGGGGFGGGBGGEEGDFGAGGFGFGFDFGGGEGFGEGGGEFGDGAGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:30:9587:17311/1 0 MtDNA 2647 60 101M * 0 0 ATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTA GGGGGGFFEDGGEGEGFGGGGGGGG;EEEEFGGGGGGGEGGEGGEEGFGEGGGGEGDGDGCCEEBFDFFFGGGDGGGGGGFGGDGGGFGGFGGGGGGFDGC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:90:16079:8007/1 16 MtDNA 2653 60 101M * 0 0 TAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGA GEEBEA5GFGGGGGAEGGGGGGGGGFGFDFGGGGCGDG>GEGGFGGAGGGGDFDGGGGGFGFFFFBFFFDFGGGGFGGGGFGGFGGG?GDGDDGGGGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:48:1800:14293/1 16 MtDNA 2661 60 101M * 0 0 TTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAG FFDFBAC?ACC>E5FFFFFFFEFACCACE?C@ADE=FEFFFFFDFFFDFFFFFFFFFDEBFFFFFB?FFDF?F=ADFFFFFEEAEEDEEEFEFABDGGDG= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:83:16330:7076/1 16 MtDNA 2681 60 101M * 0 0 ACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCAT BEE@@<AC??C>>?FD:FCFFBFEFDFFFDFFBFFFBFFFF=FFBFFFFFFFDFBFFDFFFD=D=:FFDEFFFDFF?FFFFE=EEDDDEEEC@?ACCCBD= PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387 147 MtDNA 2687 60 100M = 2360 -427 TTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGG ECDEEFFFFFFHHHHFGHJIIIGHEEIJIJHHJIIJJHGJJJJJJJIIJIIIJJJJIIJIJJJJJJJIFIIIIJJJIJHJJJJIHFJGHFFHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:23:17204:14243/1 0 MtDNA 2720 60 101M * 0 0 TTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGGTCAGTAATTTCTATTTTTACTTTTATTGTTCTTT ;;;9;:===:C;CCA=?DDDDDDDD6>C>>@@??B:D=DDDDDDDDDDD=DDDDDDDDDDDDDDD=DADDDABBBDDD=5DCD?C<DD?:DDDCCB=DCDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:42:2835:3423/1 0 MtDNA 2726 60 101M * 0 0 GAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGGTCAGTAATTTCTATTTTTACTTTTATTGTTCTTTTAACTT @C@@=AAACCEDDEED?A=?C?A>C>AAABCDDDD=CCCA:AA?CDDDDDDABDBDDB?DC=C@??ACDD:CA9ABDC=>CCCDD=D8>BBBBBDC5BBCD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:73:15087:6864/1 0 MtDNA 2787 60 101M * 0 0 TCAGTAATTTCTATTTTTACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTT ==?;B;C>=;CC?CC?>:8CA=?C:;==CBD?DDCB?B?DDBDD@ABA>ABDDBBCDAD>>CDDCDCDDDCD@A>>CDDDCDA5BC=D5DDB:B5@=>CCD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:41:4283:1044/1 16 MtDNA 2804 60 101M * 0 0 TACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAG FBCCFDEEGEEAEGEEEEFEGEGGGFGFGGGGGFFFAGGGGFFCFFDEGGGGFGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGFGFGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550 99 MtDNA 2839 60 100M = 2949 210 GTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAG @@@DFFFFHHHHHGIGHIIJJJIIIJIIDHGHDHHGEGHIEIJJIGIIIJGHGGGIIIGEEHEDGGI<AAHHIIFHHFHFHG>DFD;7>CEECACDCDCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:83:6736:2885/1 0 MtDNA 2866 60 101M * 0 0 GTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAA EEEEEFFFFDDGEGFGGGGGGGGFEEEEEGGEBGEGEEFGGEGEEEEEBBCEDEEEEEEEEEEGEEEEEEEEEEEGEBEEEEEEEEEEEEEEEEDEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:62:11372:10324/1 0 MtDNA 2896 60 101M * 0 0 CTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGA F?FFBDB?=D@:@@@CC:>5:;A;;DC>DDDD:ADDA@>B=DA?D?DDC@>=?AD?DBCBBDDCDBCBDB>>DA=??5?B?C?@?@=>@CBBBBC=>@>>B PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:91:13282:2427/1 16 MtDNA 2915 60 101M * 0 0 TACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCG CEEEEEEEEEEEEEEEEBBEEEEEEEEEEEEGGEEGEGGEGEGF>EFGEGEEEEEFGFGEGEGGGDEGEGGGFEFGGGGGGGGGGFGGGGGGEEGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:53:2586:12076/1 0 MtDNA 2916 60 101M * 0 0 ACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAACTCGT GGGGGFGGFBGGGGGGGGGGFGGGGFGGD8EEEFFGGGEGGGGGG?GGGFFGGGGGFGGGGG?GAGEGGEDAFGFFEGGGEEEBGEAEEEEEEDE=::?>C PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550 147 MtDNA 2949 60 100M = 2839 -210 TCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAG ACDDDDDDDDEEECDEFDFFFHHHHHHCJIJIJIJJIHG@GHCJJJIHGGCIGIJJIIGIIJIHHGGJJIIIDIGGIGGEIIGIHG@HGHHFFFFFF@C@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:54:12110:15974/1 0 MtDNA 2950 60 101M * 0 0 CAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAGTG GFGGGGGGGGGGDGGGGGGEFGGGDFGFGGGGGFGGFEEGFFGGEGEEBGFEGFGDGGFGEGFGGEEEDEEEEAEEEFEEGEEEEEEEEEEEEEBCCADA? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:80:9039:4187/1 16 MtDNA 3058 60 101M * 0 0 CAGTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATT :@?A@<BB;BB@AAAABBBB><AAAA@AAAAEE<EEAAAAAAAAAAEEE?EBBBB?EBEEEEEEBEBAAAAA?EBAE@EEEECEEEEEEEECBCAB=<;>@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:24:5066:4097/1 0 MtDNA 3060 60 101M * 0 0 GTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATTAT DGGGGGGGEAFFFFFFFFFFEAEEEEF?FEFEBFFFFGBBDEEEBGGEE=FFDFFGGEGEFFEFFAFDFEBFAEEDGEFGEEBGEGGEGEGDEDEEEFBEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:105:7673:2329/1 16 MtDNA 3249 60 101M * 0 0 GTTAACTTAAGTTTTAAAGTGCCAAACTTTTAATTTGGAAATGGTGGACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGA EE5DEE5FFEEFBAEFD@?==D5:C?F5FFFGGBGFFFDFF=EEEECBEEBDE:DD?:GGGDGGEGGGGGEGGGGDGGGGGFGGGGGGGGDGFFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031 99 MtDNA 3295 60 100M = 3421 226 GACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGT @BCFFFFFHHHHHHIJJJJJJJJJJJJJIIIEHIJJJJJEHIJJJJJJJJJJJJJJJIJJJHIJJIIGHHGDFBCAEEEEEDDDDDACEEDDDEDDEDDE PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179 99 MtDNA 3300 60 100M = 3308 108 CATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAG CCCFFFFFHHHHHJJJJJJJJIJIJJH>HIIJJJHIIJEGJIJJJJJJJIIJJJIJIJIJJIJJJJHHADFFFFFDCECEECDDEDDDEDDEDDDDDEED PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179 147 MtDNA 3308 60 100M = 3300 -108 TTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATC CEEEEEDEDDDDDDCDDDC@EEEDFFFFFFHHIFJIJJJJJJJIJJJIHHJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:61:8761:13140/1 0 MtDNA 3309 60 101M * 0 0 TGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCT FFFFF?EGGGGD=FGGFFGGDFDFFGGGGDFDGGGD?BDFFGBGGGGDGFDFFFFGGBDDEFEFFGDEFEEGBGEBBC?BBBBBCADBEDCB:CA?@B@@: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:72:9498:11197/1 16 MtDNA 3343 60 101M * 0 0 CAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTAC EED=DF?>>A?A=>5@?CDBBFB=EFFEEDBBFDDFEDFFFFFDDFFD;FF?FEDFDFDFFEEEBDEFDFDDBFFFDFDFEFFFFFD>EFAB>EAADDDCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:30:13606:9054/1 16 MtDNA 3373 60 101M * 0 0 AAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGAC EA5B??:EEEEEEEDEDE=DBBEEB=FBEDEEEEEFDEGGEEGGFEEGA5DEGEGGEAEGGECB=FEFF=FFGEGFGFFDGFFFFDEFGFDGGGGGFGDGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031 147 MtDNA 3421 60 100M = 3295 -226 ATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAG CDDDDDDDDDDDDEEEEEEEDCFFFHHHHJIJJIJJJJJJJJJJJJJHGDIIJJIIJIHJJJJIJIJJJJJJJJJIGIIJJJJJJJJHHHHHDDFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:79:5551:14500/1 16 MtDNA 3442 60 101M * 0 0 ACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAG 5DGEGGGGEGGGEGFGGFFFGFGGGGGGGGEFGFGGGEFGGFGGGGEDGGFGGGFGDEGGGEGEGFGGGGGFAGGGGGGGGGGGGGCGGEGCGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:118:9818:14692/1 0 MtDNA 3448 60 101M * 0 0 TTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAA GGEGGEGGGEFGGGBGGFGGGGGGGGFGBFEGGGGFFGGGGGGEGGGGGGEGEGADGGGEGFEGGGGGBGEFFEFGGFGGGGEGGGGGG?GFGAGEGGGCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:50:8060:11985/1 0 MtDNA 3466 60 101M * 0 0 ATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAAGAGATATACCAGAATTTT EEAEAC=D=D:AACA@;@=@>@<@@DDDD?CA5C?DDDDDCABCADD=DDDDDDDA=ADA?>><DCCD:DD:DD>AADDACCCCBBCAD?CBCDC?B:=BD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:56:13819:18497/1 0 MtDNA 3542 60 101M * 0 0 GCAGCAAGAGATATACCAGAATTTTTAATTATTTTGTTATTCAAGAGTCTTTAGGTTTATTATTTCTTCTTTGTAGAGGAGGTCTATTACAATTTTTTATT GGGGGGFGGGGGFGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGEGGGGGGGGGGGGGGGGGFGGBEGEGEEGEEGFEFEEEFEGEEFEA PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:69:5893:18114/1 0 MtDNA 3759 60 101M * 0 0 TATTTTATTACAAATTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATT FGGGGGGGGGGGFGGGGGGGGGGGGFGGGGGGGGDAGGGGFFFEEAGGGDGEDGDGGGDGGGFBGDFGFGGDAGGGGGGGGGGGAEFGGFEFBGGEGDCCG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:67:1744:8605/1 0 MtDNA 3773 60 101M * 0 0 TTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATTTGTTAATTATTTCA GGGGGGGGGGFGGEGFFFFFBDEBEGGGGFG8DGGDE@EEEEEE?GGGFFG?GBEGFEGEEGGGGFCCAGEEFFEDDBEFEGEFEEGGGEGEEGEEAFGFB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1310:8213:55897 99 MtDNA 3943 60 100M = 4215 372 ATTTATTACTTTGTATTAATAGTTTTATTAATTTCTAAGTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATA CCCFFFFFHHHHHFHHIJJJJJJIJJJJJJIJJJJIJJJHIIJJJJIJJJIJIJJJGIIIJHIIJJIIJJIIIJEGGIJJIIHHHHHHCEFFFFFDEEEC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:12526:7315/1 0 MtDNA 3981 60 101M * 0 0 GTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCAT EEEEEEEDEBDDFFFFEFAEFFFFFFFFFFFFFFFFFDFFEFFFFFEFFDEFEFEFFFFFFDEFFFEFFFAEBE@EEFEFFFFFEBEEFEEDFEEEE?EBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2311:10246:30315 99 MtDNA 3984 60 100M = 4165 281 TTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCATTG CCCFFFFFHHHHHJJIIJJJIJJJJJJJJIJJIJJJJJJJJJGJJJIBGIJJJJJJJJJJJJJIJJJJIJIHIJJEHIJJHFFFEDDEEEDDDE@CCDEC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:12:4535:13471/1 16 MtDNA 4089 60 101M * 0 0 AATTTTTAAATATGATAGATTCTTTACTCTATTTTTGCTTTTTACAATATTTTTATCTGTATTGGCATTTAGATTTTGATTAATTAACTTGAGAATGAAAA GGGGDADGFGFEGEBEFEFFFEEEEBEBC@EGGGGGEGFGGGGFGFG<GGGEEGGGGGGFGGGGFGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGFEGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2311:10246:30315 147 MtDNA 4165 60 100M = 3984 -281 TGATTAATTAACTTGAGAATGAAAAATAATGAAGAAACTTCAAATAATAATAAAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTT EEEDFFFDFFHFGHHHHHHGHJJIJJIIHDIJJJJIHJJJJJJJJJJJIIJJJJJJIJJJJJJJJIJJJJJJJJHJIJJJJJIJIJJHHHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1310:8213:55897 147 MtDNA 4215 60 100M = 3943 -372 TAAAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTTAATTACTTTTCTAGTAAAATATATTATATTATCTTGATAAGGTAAAGTTC @AECC@BDEDFFFGHHECHJIJJJJJJIEJIHJIJHEGIJJJJIHIJJIIJIIHFIJIJIJJJJJJJJJJJJJJJJIIJJJIIJJJIHHGHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:6:13139:10288/1 16 MtDNA 4217 60 101M * 0 0 AAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTTAATTACTTTTCTAGTAAAATATATTATATTATCTTGATAAGGTAAAGTTCCAG EDCE=C==?B=DD?5@=CDDDBBDEEDEECE5EGGDEBDFEEBFGBEEEECE=AEEADGFFGF?GFGGGGFB?BE=BGDEFGGGFDFGGGDDFFFF?DEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:35:11810:7389/1 16 MtDNA 4285 60 101M * 0 0 ATATTATATTATCTTGATAAGGTAAAGTTCCAGTTGGGAGAAGTAAGATGTAAAATAGATATTACTATGTTTGGTTACGGTCCAAAAAGATGCACATCTTT DB===?B?EEB5B5EDC?:@EABB:?B@BCBAF5BFFFFFD=DDDFEFDFFFDDFEEE:BECE?=C=AE?EABE5EEFFFEEA8EE=BECEEBB=EFFEFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:9:13349:5289/1 16 MtDNA 4305 60 101M * 0 0 GGTAAAGTTCCAGTTGGGAGAAGTAAGATGTAAAATAGATATTACTATGTTTGGTTACGGTCCAAAAAGATGCACATCTTTGCGATCTAGTTTAGAAAAAA =GEGGFGGEEGGGGFAFFGEGGGFGEGGAFGGGGFGDGGFEGFGGGFFGGGGGGAEFFFFBDGGGGGGGGDGFGGDGGGGGGGGGGGEGGGFAGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:31:10170:14522/1 16 MtDNA 4388 60 101M * 0 0 GATCTAGTTTAGAAAAAATATTTGTTTTTGGTGCAAAAGAGTTTGATTGCATTTAGTTTACTCTTTTAGTTTATAATTAAAATATGGCCCTGAAGAGGCTA EEE?DE5FDECCCC=FEE?AGEGGFFGGGFGGGFFGFGGEBBGGGGFGGBGGGDGGDDGGGGGGEGDGFGGDGGGFGDGGGGGFEGGGEGGGFGGGEGEGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:7488:8665/1 0 MtDNA 4539 60 101M * 0 0 TGGGATGTTGGTGACATTGCCATCTAGAAAAACTTTAACATTAAGATGAAATTTTGGTAGTATATTGGGTATAGTTTTAATCTTTCAGATTTTAACAGGTA GGGFGFGFFGFEFDFFDFFEGEGGGGFGEFGGGD?GGDGGGGGGGGFFGAGEFDGDDBEEDFFFEGGGE@DEEEBCDFBEFGGFGFGFEDE?E?GFDEFAF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:49:13216:3175/1 0 MtDNA 4636 60 90M1D11M * 0 0 GGTACATTTTTAGCATTTTATTATACGCCCGATAGGTTAATAGCATTTTCAACAGTGCAGTATATTATGTATGAGGTAAATTTTGGATGAACTTTTTAATT DD@DDAEEEDGGFDGFGGGDGGGGGGEGFGBDFEFEDFFFEFFFDGGGFGADFFFBDDCECEECEFEFDCEFGEGEA?C=DDECED?EF@EAEEEEEEEFB PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:21:4522:8165/1 0 MtDNA 4827 60 101M * 0 0 AGTATGAATGTCTGGTTTAACAATTTATTTATTAGTAATAATAGAAGCTTTTATAGGTTATGTTTTAGTTTGAGCTCAAATAAGATTTTGAGCAGCAGTAG DEEDECBEEEBEEE=?EEEEFDAFFEFEE<EEDEEFEFDFDFFF?FFFBFEDDEEFFBF5D:BDDD>=ADEC:DAFFE=FFFBFBF?FFD:FDFFFDFADE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:84:1391:9200/1 0 MtDNA 4874 60 101M * 0 0 CTTTTATAGGTTATGTTTTAGTTTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACGATT GFGGGFFGDDEBEEEFFFFEFFFFFGGGGGGGGEDGFGGFGGGGEGBGGGGFGGGGAG?EFFDGGFGGGG:EEAD=ECEEEGFGGFFFFEA?CEABB7BB@ PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:107:7520:19689/1 0 MtDNA 4896 60 101M * 0 0 TTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTT BGGGGADGGGGDGGFFAGGF:FFFFGFGFFFACDEFFFEBGF?GGGFGBEEC5DED==D??ECEEEFGGGFFEFBD=BAEDDBFBD:?BDEEDB?===D@? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:31:10465:15263/1 0 MtDNA 4937 60 101M * 0 0 GACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTTTTGGTGTTACAGGGGCAACATTAAAATTCTTTTTTGTATTA GGGGGGGGDGGGEGGE=EEEDGDGGFGDGGGBGFGGGGGEGDGEEGEGGGGEDEGEEE=EEEDE=FAEFAEEFDEBGFEDEEEEBEBEEBEGEE7C:=5AB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:29:14125:10562/1 16 MtDNA 5109 60 101M * 0 0 AACATCTAGGTTATATTGCCACGGTGATTATGATAAAGTTTGTTTTAGACCTGAGTACTTAGGTAAAGATGCTTATAATATTGTTATTTGATTATTATTTA ECGDEGEGEGB?FBGFEGGGCGEGDGFGFG?GGGGFEGG6GGGGDGGGEGGEEEE=EGGGEGGGGFBEGEGGBFGGGGDFFGFFGGGEDGGEGGGGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:37:12921:1122/1 16 MtDNA 5174 60 101M * 0 0 AAGATGCTTATAATATTGTTATTTGATTATTATTTATTGTGTTAAGGTTGATTTACCCGTTTAATTTAGGTGATGCAGAGATGTTTATTGAAGCTGACCCT DAB=?B=BGGEGBEDGEGEGGGGDGEGGFAFBGEGGG5EGGEBGGGCE?E?GGECGDEFGGEGGGGFGGDGGGFGDGGGGFFGGGGDGFGGGFFGGDFFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082 99 MtDNA 5381 60 100M = 5391 110 GGATCGTAACATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTAC @@=DDDDDFHFDHIIHIJJJJJIJIIIJJJGIGIJGJJJIJJJJJIIJJH=FGHJICHHIJJGIJJIIHEEHHHF@EFFFFEEEEEEEEEFDDDCCCDD: PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082 147 MtDNA 5391 60 100M = 5381 -110 ATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTACTATTTTAAGA =CCCCBBBEEHEHCFIEAGGHIFGC@IGGHFCIIIGIGD@IHGIGIHEEIHFGIIIIIDIIIIIIIIGFC:IIIIHECIGEFEC<GIHFFB?DDDDD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:70:6358:2346/1 0 MtDNA 5567 60 101M * 0 0 CTTATTTAATATTGTTTATTTTTATAAGAAGTAAATTGTTATTTAAATAATAAAACATGTCTAGTATAAGTTAATACATTAGATTTAGGTTCTAAAGATTT FFGBFGFGGDGGDFGGGDEACCACAGGGGEDEGFGGDFFGDEGGDGGFG?GGGGGGEGGAEEDEBFDFDFEEGDBFFFFFDGEEDEBEE@EGEEEDEDDEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:5:6773:20401/1 0 MtDNA 5728 60 101M * 0 0 ATTTTTTGCCTCAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTT GGGFFFEFFGGGDGGGGGGFGGGFGGGGGGGGGEFFGGFGGGGGGGGDFGFFFFDEFDFBEEEDBEEEEEAGGGGDCEGGEDGGDGGDGFGGGDGGGDGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:39:10545:13693/1 16 MtDNA 5756 60 101M * 0 0 TCTTTAGTAATATTTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTAT FADAAEEEF;CDFAEEEEFEAEEEB@>CCCA:EE?EF=A@ECFFFFD?EEEEDEE:EFFEFEEEBAC;FECCDBDEEEBA??F?DFAFFFEEDDE=DDAD: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:96:18019:19876/1 16 MtDNA 5769 60 101M * 0 0 TTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGA GEBBFGGFEEGEE=EBGFEGGEEEEADDGGGEEEEBEAFFEFFDEDGG?GGGFGFG@DGGFGGBGFGGEGFFGGGGGGDFFGBGBGGGGGFGFGGFDDFEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:28:5015:7424/1 0 MtDNA 5798 60 101M * 0 0 ATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGG GGGGGGFGDGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGBFFFFFGGGGGGGGGGGGGGGBGGFGFEFFCFAEFFDDGFGGFGFEGEGFFDFDDFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:30:5276:1934/1 0 MtDNA 5801 60 101M * 0 0 TTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTT GGGDGGGGEGGGGGGGFGGGEGGGGGGGGGFGGGGGGGGGGDGDGFCFCFGGGGGGDGDGGGDGG=EDBEFAFDEDEFBEGGGGEGEGEFCECEC?EEECC PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059 99 MtDNA 5853 60 100M = 5996 243 CTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTTTAAATTCGGAGTAATTTTATTTGTGTTTAGGGAGTTTATATTCTTCTTTTG BCCFFFFFHHDFHIJJIHJFHJJJJJJJJJJJJJJJIJIJJJIJJII@FIHIIIIIJJHH?AEHHHFDFFFFFEA6@>CDD;?<CCCDDDFEEEDDCDD: PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:83:3624:6531/1 16 MtDNA 5868 60 101M * 0 0 GAGGTTACCATAATTTTTTCGTTATAGACGGGTTTAAATTCGGAGTAATTTTATTTGTGTTTAGGGTGTTTATATTCTTCTTTTGTATTTTTTGAACATTT BEEDFDEGDEEFEEDBDFEGFGGFGGFBGFGFAGGFGGGFGGGEGGG>EGGGGGGFGGGGFEEEEF;EFEEGGGGFGGGGGGGGGGFGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:76:4139:2647/1 16 MtDNA 5966 60 101M * 0 0 TTTTTTGATGCTGATCTAGTACCAGTACACGAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATAC EBBEEEEEEEE@B@BEB@@B?BC?BB@B?DEEEDEFDBEEEB=CCCE@EE@FFFFDECEEDEDEBEFFEFDFFFEFEAFDFFEFCFBDFFFFFFAFGGGBG PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059 147 MtDNA 5996 60 100M = 5853 -243 GAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAG CDDDCCAECEC@EEFFFFFDAHHGJJJJJIJIJJIJJJJJJJJJJJJIGHHGJJJIGJIJJJJJIJJIIHJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:99:8553:16567/1 0 MtDNA 6020 60 101M * 0 0 TTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACT :FDGBFGFGDDGFDGFFGGFBGGDGFFFF=EAEEEDEEAEFDFDFGFG?GG?FEGGGFGGGGGFFDFFEFEAFFFGEGGGGAGEFGEFGGFGGECBEDDFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934 99 MtDNA 6028 60 100M = 6185 257 GCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAAT CCCFFFFFHHHHHJJJJJJJJFHGHIIJIJJJJGJJIIJJJIIJJJJJIJJIIJJJJBFIBFGHIEIGEGHJJJJJHHHHGHFFFFFDECECEEEDDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:83:16211:16889/1 0 MtDNA 6029 60 101M * 0 0 CACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAA EAEEBGFGGFGGGGEFFFAFEFFGEGFEDGDEECCGFDFBGGEGFGG@FGCBFDDFDFDBBDDBBBEBEFBAEEBFBDFEEGEEEB=B@BBEB?BAABDBE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:8:8735:6148/1 16 MtDNA 6076 60 101M * 0 0 ATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAAAAGATGTACTAATAGTATAATTTTAACATGTTTATTGGCAGCTTATT DB:DDDB?D:BDCDDDD?DDDD=DADDADDBDDDDBDDDDDDADDDBC>CDDADADDD:DBCCBDDDDADDDDDBD5DDDDE?DEE:EFFEEEBDD;EEE: PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934 147 MtDNA 6185 60 100M = 6028 -257 ATTCAATTAATAGAGTATATAGAAGCTAGATTTTCTATTGCAGACGGAGTATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCT CCACEEFEECDCDEEEEEEEDEFFFFFFEGHHHHIJJIGJJHHDJIJJIHHJJJJJJJIGGJJJJJJJIJJJIIHGJIJJJJIIIGJHGHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:105:1340:12432/1 0 MtDNA 6235 60 101M * 0 0 ATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCTTGTGTGGTGGTTTGTTCTTAGCATTTAATTTTTTACGTCTTCTAAAAAATC FFFD@DGGEEEFGGGGGGDFGGGGGGGEGGFEFFEEDEE:EADDEFF5FBEE=EEDDADDB?DE=><:6>EEDEE:D5EDEEBB5:ECAC:?B:CEBEEEC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:54:12468:5717/1 16 MtDNA 6304 60 101M * 0 0 AGCATTTAATTTTTTACGTCTTCTAAAAAATCATTTTAATTATAATCATCATCTGGGGTTGGAATTTGCTATTTTATATTGACATTTTGTTGATGTAGTGT BBB:DDC?CBCB@AB:BDDDD@C?>CACB::?DCD=CC:?A=D:DB?DDAD:?CCCD=D:DDDDA?ADDDA;C:AAC?=A??:@<DC5CC=A5CCA6;@=; PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:9266:7772/1 0 MtDNA 6313 60 101M * 0 0 TTTTTTACGTCTTCTAAAAAATCATTTTAATTATAATCATCATCTGGGGTTGGAATTTGCTATTTTATATTGACATTTTGTTGATGTAGTGTGATTATTTT GGGGGGGGGGEDFGGGGGFGGDGGEGFFEGFGFGEGGGGGGFGGGGGGGEFGFDEDGGGGGEFGGFBGGGGGAEBBFFFEEEEBEEBBEAEAEABGEEDGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:65:9093:5871/1 16 MtDNA 6392 60 101M * 0 0 GTTGATGTAGTGTGATTATTTTTATTTGTGTTTGTTTATTGATGATCTTACTAATATAGCTATGATAGTTTAATTTAGAATATATAACTTGTAATTATAAG FBEEBE??:B@D:FAFFEAAFFFFFFFFFAEFFEFCFFFFF?FEFFDEEEDDFFEFCCCC:DDDDADEEEEDDBEEEEED=FFFFEEFDGGGGDGGEGGBG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:89:15660:12072/1 16 MtDNA 6413 60 101M * 0 0 TTATTTGTGTTTGTTTATTGATGATCTTACTAATATAGCTATGATAGTTTAATTTAGAATATATAACTTGTAATTATAAGGTTTCTAGTAGCTTTGTTAGA BEEAEEEGEGEEFGEGFGBGDFGEBFGEEGFGGFGGGGGFFFFEDCEGGGGEDEFFGGGGDEEED?AGFGGEGDGFGEFGGEGGGFGGGGGDGBGGGAFGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:8873:86961 99 MtDNA 6488 60 100M = 6541 153 ATAAGGTTTCTAGTAGCTTTGTTAGAATTTCTTTTTATCTCCTTATTATGACTTTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTT @B@FFFBDBHHFHEGGIGIECEHIJGHIIGGIIIJIGIJIJGDHHHIJJEDEEGIIIIIIJJJJJJJJJIGGIIJJIHDECHEEE?DFF@?CFECE>CCB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:35:14137:12613/1 0 MtDNA 6539 60 101M * 0 0 CTTTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATA GGGGGGGGGGGGDGGGGGFGGGGGGGGGGFFGFGDGCGGECFFFF?FEFDEAGGGAGGGGGGGGGGDGFGGEFFGGGEEGEGDGGE@GEEGEEFEEAEED@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:8873:86961 147 MtDNA 6541 60 100M = 6488 -153 TTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATAT CACCCCCCCDA@CCA<EEEHFHEA=>CAIGIGH@;F9EEFC1EIHHCEHGBCIHFAGD9HD?9DCFFCGCCA@A,ACGEIIGGGEDE?F?DFABD7D@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:80:12688:9364/1 0 MtDNA 6564 60 101M * 0 0 TATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATA GGGEGAFCDDFAFDFEECDE@EEDEEFFFBDDDD:A?AC?GDGEFFGFEACEB:C=EEEE>:C@BEEEE=DEFA?DFEE?DE??EFC>5B;?AAEDAD9D= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:83:3138:12153/1 16 MtDNA 6612 60 101M * 0 0 GCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAAT GGGFEEGFEEEBEDGGFGFFGGGGGFGFGGEGFGDGGGGGGAGGFGGGGGGGGGGGGGGFGGGFGGGGGGGDGGGGFGGBFGGGGFGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:15380:6072/1 16 MtDNA 6635 60 101M * 0 0 TCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTT EDGEEBEE@EEGEEEEEEEAEEE?EEEEAEBFEEFEEEGGGEEGDEEFEDDGFEECGGEDGEGEDGEGDGEGFGEGGGFEECDCD;G=GFFAGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248 73 MtDNA 6659 60 87M1I5M1I1M1I4M = 6659 0 GTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTTAGTATTTATTCTGTCTCTTATAC CCCFFFFFHHHHHJJIJJJJIIICHJJIJJIJJIJJJJJJJJJJJJJJIIJJIJJJJJJIEIJJIIJJJJIGGHJGHHHFHHHFFFFFFFEEEEEEDEDD PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248 133 MtDNA 6659 0 * = 6659 0 AATAAATACTAAAATTTCAGATAAAATCAATAAATTATTATTTTTTTCTCTAATAACAATAATACCTAAAATAAATAATCTTATAACCTGTCTCTTATAC CCCFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJIJJJJHHHHHHHFFFFFFFDDECEDDDDDDDC PG:Z:SNAP RG:Z:000 +EAGLE:1:39:10037:14104/1 16 MtDNA 6823 60 101M * 0 0 TATAATTCTAGGTTATGGTTCTCAAATTGAAAAAATTAACTCCTCTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATT E?F?FDFGF?FGAGAGFFFEDGGDGGGFGFGFFF?FG?EGGDDBDBAF>EFEGGGGCGDGFGGFGGFGGGFGGFGGFGGGEDGGFGGDDGFFGEGFFGFDF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:51:9949:15014/1 16 MtDNA 6862 60 1M1D6M1I87M1I5M * 0 0 CGATCTTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATTTTAAGAGTAATTTTTTATTGGTTTTTTCTTACTTATTAC EEEEEEEBEEEEEE=EEEEEBEEEEEEEE=C?EEDDEEDEE?EEECCCCEDDEEEEBEEEEEEEEAECEBACAC<EEEEE=ECDEED?DDBE5AEEEEEDE PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:19195:39418 83 MtDNA 6948 60 100M = 6949 101 GTACTTACTATAATTTTGTTATTTCTTGAGAAATATTTTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACC CCBDFDDDEEEDEDEFFFFFFHHFHHIJJJJIJJJJIJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJIHIJHHHHHFFFDD@BB PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:19195:39418 163 MtDNA 6949 60 100M = 6948 -101 TACTTACTATAATTTTGTTATTTCTTGAGAAATATTTTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACCC CCCFFFFFHHHHHJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHIGIJJJJJJJJJJJJJJJJJJJIIJJJJIJJJJJFFFFFFEEEECEEEC>; PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:86:3647:16273/1 16 MtDNA 6985 60 101M * 0 0 TTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACCTAAAGCTCATGTAGAGGCTCCTACAACAGCTAGAATAC <DEBBBEEEEBEBBEEEEEEEEEEEG?FGEEDD?EBEEEEEEEFEDEDAFEBDDEEEDFFADCBCCFABFEGFGDGEFEDGEFFFEFDFFFEFFFFDEEBE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:10706:29128 99 MtDNA 7118 60 100M = 7195 177 GCGGGATTTTTACGTATTTTAGGTAGTTTAAGATTTGTAAAAAACAATGTATGAATTTTTATTGCATTTTTAGGGATAATTTTTGGATTTTTTTGTTGTT ;;<@################################################################################################ PG:Z:SNAP RG:Z:000 NM:i:6 +EAGLE:2:99:13522:17668/1 0 MtDNA 7120 60 101M * 0 0 GGGATTTTTACGTATTTTAGGTAGTTTAAGATTTGTACACAACAATGTATGAATTTTAATTGCATTTTTAGGGATAATTTTAGGATCTTTTTGTTGTGTGT GFDGFGGEAFEFBFF?FFFFDBDF?ECECEEGDFGEGGGGFEEDDFGEGEBDEFDBEEFBEGFDEFEEE9EEC@?EEEEEDEGEEEEFEEGGEEEABBAFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:10706:29128 147 MtDNA 7195 60 100M = 7118 -177 AATTTTAGGATCTTTTTGTTGTGTGTTTCAAAGAGATTCAAAGGCTTTAGCAGCTTATTCTTCAGTCACTCATATAAGGTTTTTATTATTATCACTGGTA CEDDDCC@;DBCCDEBFDFHFFJGEGIGGIJJJIEIIGHEIHGJGJJIHFIHACGGEGIJIGIIHHEFIGEJIJGIHEJIJJIGIGGGGHHHED>FDC@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:102:7363:18384/1 0 MtDNA 7214 60 101M * 0 0 TGTGTGTTTCAAAGAGATTCAAAGGCTTTAGCAGCTTATTCTTCAGTCACTCATATAAGGTTTTTATTATTATCACTGGTATTTATCACAATGAGTAGTAA D:BDDCEDEEBFFFFGGEGGGGGGGGGGG:EEEEEGGGGFGFGGCGEGGFGDGGGGGEEGBEEEEFGGEGEAFE:FFDFCGGGGGFFADDFFFFFAFFEEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:38:3103:6670/1 16 MtDNA 7304 60 101M * 0 0 ATGAGTAGTAAAATTAGAAGAGTTATGCTTATGCTAGCACACGGTTATACATCTACACTTATGTTTTACCTTATTGGTGAATTTTATCATACAACTGAAAA FGGEGGGGGGGGGAGGGGFGGGEEGGGGGGEEGGGGGGFGGGGGGGGGFGGFFFDFEGGGGFGGGGGEGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:55:13359:5768/1 0 MtDNA 7379 60 101M * 0 0 GGTGAATTTTATCATACATCTGGGAGGCGTATAATTTATTTTATAAGTAGATTTTTTAGATCTAGAATAATTATAGGTATTTTGTTTTCAGTTGTATTTTT FBFEFFFADEGBGDGDGGGGGFFGGGGFGDGEBBGGGFFGGGGAGEEBFFFGEGFGGEBEGGGBGEEGDFDGFGEGGEAGFGGGDGFEEBCEEECEFGGG? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:40:8204:14676/1 16 MtDNA 7463 60 101M * 0 0 TTTTCAGTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTAT DDBEBGED?FGGECGGECDE;FGEGGFGGDGFDFFCFF>FFGGGGGDGGGEGGGGGGGGGFDGGDGGD?GGDGGGGGGGGGFGGGGGGGEGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717 99 MtDNA 7469 60 100M = 7536 167 GTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTATATTTG CBCFFFFFHHHHHIJJJJJJJJJEHGHIIIIJIIJJJJJIJJJJJJJJJIIIIHIJJIJIIGIJJJJJJEGHGGCCEHHHHHHHFFDFDDCD;CCBDDE> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717 147 MtDNA 7536 60 100M = 7469 -167 CTAATAGTATATTAATTAGAAAAAGTATATTTGTAATAATTTTTATTTATTTTGTAGTATCTTTTTACTATTCTTTATTTTTGATTACAAGATCTTTAAT DEEEEFFFFFFFFHHHHHHJJJJIJIJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJJJIIJJJJJIGJJJJJJJJJJJJJJJJJJJIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:57:7347:8572/1 0 MtDNA 7620 60 101M * 0 0 TTACAAGATCTTTAATAGGAAAAGGTTACCATAATTTTAATACTTGAAATGTAGGGTTTTCAGCACCATTAGTCTTAATAATATATAATGTATTTTGATTA FEFFDEBE??EEDEAFEDFDFFFDEBBDEEC?CCD=EEEBFFEEFF?FF=?:?ACE?BCEFFFFDFEFDEFEEEDEBDDEEEDEEEDDED@B=ABEDDBEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588 99 MtDNA 7885 60 100M = 7994 209 GATTAGAGAGATCTAATCATAAAGATATCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTT B@@FFDFE>FFHHIJJIGIIJJIGHGJJJFIIIJJJJJJHJIJJGIJJJIGHIGIIJJIIIJIGCGHGDEHIE?CEFFFEFFEDEEEEDEDCDEEDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647 99 MtDNA 7912 60 100M = 8034 222 TCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTTAGAATTAGCTAAACCAGGTTTTTTTCT BB<1:=@BFHFH>CFHB<F<CHD@:FB?HEA<?CADHI@DFHHBEB0?FGCFHEAFEEEHI@FFDHJJ@;@=@@>>EC;=;@EEB;@>ACD555=?=BDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588 147 MtDNA 7994 60 100M = 7885 -209 TAAACCAGGTTTTTTTCTTAGGAATGGACAGTTGTATAATTCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATC CBC@DBCBDDDDDEDEEEEFFFFC?BDAFHHEIJJJJIHGGGIIGIIIIHJIIIGFJIIGJJJJJJIIGIIIJJJIIIIJIJJJJIGFGHHFFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647 147 MtDNA 8034 60 100M = 7912 -222 TCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATCGGTGGTTTTGGTAACTGATTATTACCACTTATGTTAGGAG :::@@B;5AA:@:@5:A>>A>;B@@@>DA>@?6AC;7C@=8B<FCF>D<FF?IIG@GG?@DGEGD@EA<4@F?<AC3,<F:<:A@C:FBF8?AD?DB@@= PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:86:14256:19867/1 16 MtDNA 8225 60 101M * 0 0 TATAGGTTGTGGGACTAGGTGAACAGTCTACCCACCTTTAAGAACAATGGGGCACCCTGGAAGTAGAGTAGATTTAGCTATTTTTAGTTTACATGCAGCAG =BBB@BBBBBDBDBBBAD@ABDADBDABA6BBDBDBDDDDEEGEEGDDFEFEE@EEEGGGFDGGFGGGGGGGGGFGGGFGGEGGGGGEGGFGGGEGGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:18868:93712 99 MtDNA 8250 60 100M = 8386 236 GTCTACCCACCTTTAAGAACAATGGGGCACCCTGGAAGTAGAGTAGATTTAGCTATTTTTAGTTTACATGCAGCAGGGTTAAGATCTATCTTAGGTGGTA 11:BA::2220CFH@>94:<<:H@BBEHHGHHGGI?GEH>?FADFGIGIICGGEGIBAHGIICFHEEC>DGEHGGHFF@BDEE@CACCCCDDDDD::<?3 PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:18868:93712 147 MtDNA 8386 60 100M = 8250 -236 GTTCTATTTCATTAGAACATATAACTTTATTTGTTTGAACTGTGTTTGTAACAGTGTTTTTACTGGTTTTATCTCTACCGGTTTTAGCAGGGGCTATTAC AB>:>A>@A>@;EEEEDDEEEDAB@DDDDDB;?=?:IEECAEC;@CBDF==FCFB?FBBD<G=BDDDBAGEG@FGC<GFAHI<AEA;C:<==;D?DB@=@ PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:39:11175:12286/1 0 MtDNA 8405 60 101M * 0 0 TATAACTTTATTTGTTTGAACTGTGTTTGTAACAGTGTTTTTACTGGTTTTATCTCTACCGGTTTTAGCAGGGGCTATTACTATGTTGTTAACTGATCGTA BDDD?BDDDDEEEEFFFFEFFFFBFEEDEBEEEEEDFEFFFCCF:EE@EEFDEFDFFEFFFFDEFBEDEBDD:FBEEEEBEEEEAADDBABDADFB?C=<B PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:113:8946:21007/1 0 MtDNA 8550 60 101M * 0 0 CTTATTTATCAACATTTGTTTTGATTTTTTGGTCATCCTGAAGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAAC GGDGGGGGGGDAGBEGGGFGGFGGFGGGEGDGEG=FFFEFGEFGEGFEEGGGGGFDGGGGG?FGEGGGGGECEFFFEEEGBEE?DFEADC?DEE=BE?<B5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:81:3391:15213/1 16 MtDNA 8553 60 101M * 0 0 ATTTATCAACATTTGTTTTGATTTTTTGGTCATCCTGAAGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAACAGG ?DEDDDGEBE@EEFEFFGGGEGGGFGGGGEFEEGGGGGFGEGGGGGGGGGGFGGGGEGGFEEFFGGGGDGGGGFGGFEGGGGGGEFFGGDEGGGGGGFEGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:2101:11733 99 MtDNA 8591 60 100M = 8633 142 AGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAACAGGAAAAAAAGAAGTTTTTGGTGCTTTGGGTATAGTTTAT BC@DFFFFHHHHGGJGJJJJJJIJJJJJJGDHIJGIEGIIIHGGIICGCDDG>GIJIIGG@DHHG9FGIBHFBEDDFEDD?BDDDDDDCB5>>CEDCDCA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:2101:11733 147 MtDNA 8633 60 100M = 8591 -142 ATCTACACTTTATTTAACAGGAAAAAAAGAAGTTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCT DDA>@C@DCAEECC>CDCDDDDDHHHHHHEGHEEGGJJIJHGGJIGGGIIHCIGIIJIIGHAIJJIJJJJIGIIIJJJJJIIIJJJIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:25:12122:5259/1 0 MtDNA 8665 60 101M * 0 0 TTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGAT FGFAGFDBFD:EEEEEB5AEEEEEAFFFAFDF=FEFEFFFFDAEEFFFFFD:FFACEB:E?:BD?B?EBAFADBBDDBCEBBBAEBEE@EBBE@BFFE?AF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:82:4393:14891/1 0 MtDNA 8674 60 101M * 0 0 CTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGATTCACGTGCT DGFDDEEAEEEECEEFFEDFGGGGGGEGDFEEEDEDEDBEGEGDGEEEEEFDFFFGGGCBFGGGBFFFDDFDFEFCEEE5@B?D:?CBDB5@B?@CFCF?B PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:3:4300:2958/1 0 MtDNA 8776 60 101M * 0 0 ATTTTTCGGCTGCTACTATAGTTATTGCAGTGCCAACAGGTGTTAAAGTGTTTAGATGATTGGCTACATTATTTGGTATAAAAATGGTATTTAATCCACTT GGGGGGGGGGGGGGGGGGGFGFGGGGGGGDDFFGGEEEEECEEEEECE@EFFFBFAEGBFGGFFFFDEDGCGGGBD=@@D:AA;9EE??=AA@C55>;<?E PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:75:3836:14170/1 0 MtDNA 8880 60 101M * 0 0 TTGTGAGTATTGGGTTTTATTTTTTTGTTTACTTTAGGTGGGTTGACAGGTGTTGTATTATCTAATTCAAGATTGGATATTATTTTACATGATACTTATTA EEECEEEEEEDDDDBGGGDGGGGGEFGDFGFGGGFGFG@ACC<CCBEEEE?EEAEDFFFFGGGFGGGGFEGBEGGDEGGEGEGGGGGGGGGEGGFFGGFGD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:57:17429:11489/1 0 MtDNA 8902 60 101M * 0 0 TTTTGTTTACTTTAGGTGGGTTGACAGGTGTTGTATTATCTAATTCAAGATTGGATATTATTTTACATGATACTTATTATGTAGTTAGACATTTTCATTAT GGGGGGGGEGGGFAGFDFFFEEFFFFFFCFFFFDFEBBFEGFGGGEEEGECGGGCGEGEAEGGEGGBGGEDEEFEDGGDGACCECFDBBCFEFEFD@AFA@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:8770:13442 99 MtDNA 9086 60 100M = 9229 243 ACTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATAT CCCFFFFFHHHHHJJJJFHHJJJJJJJIJJIJJJJJJJJJJCFHIJJJJIGJJJJJJJJJIJJIJJJJJJIIIGHEHHHFFFFDDDDDDDDDDD?ABFDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:14393:27976 99 MtDNA 9087 60 100M = 9151 164 CTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATT @C@FFFFFFHHHHJJJGIIIJIJIIJGHIGIGIIIIJGIJ?AGHJGIIJHIIJIJJJJJIJJIJJJJJJIGIGGEAEHEDFDDD>?BDDCDDBDDBDEDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:50:14046:18753/1 16 MtDNA 9087 60 101M * 0 0 CTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTT BEEEEEEEEEGEEEEEEEE?D@EGGGEEEEGEEEEEGEGEEGDEGGEEEGEGGFGEGGGGGGEGGGGGDGGGGGEGGEGGGGGGGGGGGGGGGGGGGAGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822 99 MtDNA 9102 60 100M = 9126 124 GTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATG CBCFFFFFHHHHHJIJJJIJJJJJJCFHIJJJJJGHIJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJIHBEFFFEBBDDDDEEFFEFDDDEDDDDDDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:72:10661:11179/1 16 MtDNA 9109 60 101M * 0 0 TTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCG ;EEBEEEEEEEAEEEEEEEEEEEEEEBEDDD@E?EEGEEBEEDEEGDEEDEDCFFCECFFEGGEEGDEEGEFGGGGEFGFGGFGCGGDGGGGGGFGGFFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822 147 MtDNA 9126 60 100M = 9102 -124 GTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTG EDDEEDEEEDDDDDDBBDBAADEFFFFFBHHHHHHGJIHIIJJJJJJIIHJJJJJJJJJJJJJJIHG?JJJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:14393:27976 147 MtDNA 9151 60 100M = 9087 -164 ATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAAC CCC@DDCDDEDCCDDBDDDFDBFGFEIJIIGIGJJIJJIHGC=HIIIGFCJJJIIIIGJFIJJIJJJJFIHGJIGDHGIIIGHGIGIHHHHFFFFDD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:52:2501:13323/1 0 MtDNA 9191 60 101M * 0 0 TTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTT GGFFGGFGEGGGFGGAFFFFGGGGGGGGEGGGGDGGGGGGGGGGFEGGGGGGGGGGGGGGGGGGGGEGGEGGGGGGGGGEGGGEGGEGGGGGGEFEFCFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:46:14009:4930/1 0 MtDNA 9196 60 101M * 0 0 CTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTT EEDBEDEFEFFFFFFEDEEBFFFDFECBE?BEEEEDCCEAEGDG=FEEDFAA:BEB=BBCA=AAAEAFBFFEGGBDF?=FABCCBEAGD=????@EEABE; PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:8770:13442 147 MtDNA 9229 60 100M = 9086 -243 CTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTC CDEEDDEFFFFFFFHHFHHHFJJJJJJIHJJJJJJJJJJJJJJJJJJIIIJJJJJJJJJJJJIJJJJJJJJJJJJIJJJJJJJJJJJHHHHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141 99 MtDNA 9258 60 100M = 9340 182 CTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTA CC@FFFFFHGHHHJJJIJJJGJJIJJIIJJHJJJJJIJJHIHIJIJIHHIHIIJJJJJIHIJJJJJJJGIJJGHGHIDHIIIIIIGIEHDHFHGFFDFDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:4177:11048/1 16 MtDNA 9303 60 101M * 0 0 CGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAAC DGEGEEGDEEDGFGEGGEGGGFFGDGGFFFFFFFFAGFDGGFGGDFGGGGGGGGGFGFGGGGGFDGGGGGEGGGGFGGGGGGGGGGGGGGFGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141 147 MtDNA 9340 60 100M = 9258 -182 CTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAACTACTAGATTAAAAAATTAGAAACCTTAGTATAATTT CDDEEEEEFFFFFFFHHHHHHJIHDJJIJJJJIHJJJJJJJJJJJIJJJJJJJJJIJJJJIIIJIHJJJJJJJJJJJJJIIJJJIHJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:20758:28262 99 MtDNA 9506 60 100M = 9991 585 GTTCATACCCTTGAGGTGGTTTTCTCTTATTAAAAGTTTTAGTATAAAAAAGTATATTTTATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTAT CCCFFFFFHHHHHFHJAEHCDHIGJJIJHIJGIHIJFHIJHJGIIJDGHJJGBFHIJJJIGHIGIIIHBDHGGIEACHHFFFFFEEEEEEEDCEDDCCA; PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:76:8573:4029/1 0 MtDNA 9566 60 101M * 0 0 ATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTATAATTCAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAA DDDDDDD=DDDAB>CD?DDDDDDDDDDBDDDDDDDDDBDDCDDDDDDBDDDBDDDDDADDDDCCDBB=BDDBDDDD?DDBBCCCCCCDDBCBCDBDBCCCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:16574:9800/1 0 MtDNA 9610 60 101M * 0 0 CAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATAT GFAFGFDFFEFFFDDFGGGGGFGCGED=EEDDDDDEGGCGEGGGGGDFGDEFFCE?EGFEGGGDGGFGBGGCGEDGGFG?FDFBFFDGC=AEEAEDDFFDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:39:12621:19315/1 0 MtDNA 9636 60 101M * 0 0 GTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATATAGATTGATTTCATAGGTTTAATTGTA DBEEEEEEEB?E:EEFFFFB:CDCEFFACFAEEBEBCB?CFEFEFEFFFFFFFDBAC@BC=C;?<EBBEEBDCBBDFDEEB5ACC??B:BE@E?@@A@AA: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:97:3517:19360/1 16 MtDNA 9687 60 101M * 0 0 GCATAGTTTATTTGCTAGTTATATAGATTGATTTCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAA =DF=DEGGGGFGDGFGFF?FGGEGFG?GGGFFGGGGDBGGGFFFEGGGGGGGGGGGFGEEGGFGGGGFGGGGGGGFGGGEGGGGGGGGGFFGGAGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:113:4491:15082/1 16 MtDNA 9710 60 101M * 0 0 TAGATTGATTTCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAA =BEE?EEE=EEA=5EEEEEEEAEEEEDD;EE;AEE?=EEDDCE=EED?BEEEEE?EEEBDEEEE?A=DEE6EEE=EEEAEEDDEEE?@EEEGFGDGEFF:F PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:73:1194:5250/1 16 MtDNA 9720 60 101M * 0 0 TCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAAAGAAAAAAAA FF=FBFEEFFFEFFFDFDFCFEEFFDEFFFBFFAFFDFFFFFFFFFEEFFFFFEFFFFFBFFFDFFDFFFDEEEEEEECDEFFFFDFDFFFFFFFFAFFDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:24:12750:7270/1 16 MtDNA 9752 60 1I5M1D95M * 0 0 GATTTAACTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAAAGAAAAAAAATTGAGTATCAGTTTGGTGAATTATTGTGTAGT =5EAEE@EEE?FFFAFED:DBEGDEEGDFFGBFEGGFBFFFEED?>C:E8FG:GDCGGG<GF<GAGBGGDGGDGGGD=GGFFGGGFGGGGGGEBGGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:39:6442:9317/1 16 MtDNA 9797 60 101M * 0 0 CTTTTTATTTTAAAAGAAAAAAAATTGAGTATCAGTTTGGTGAATTATTGTGTAGTATTTTTCCAACTATTATTTTATTAATACAAATAGTTCCTTCACTT D8@=CADGEBDGGEEEGGFFEEEFAFG=EFEGGFFDGGGGDGGGGGGEAGGGFGGGFFGGGFFFEDBEGEGGGEGGGFGGDGDGGGEGFGGEGGGGFDFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:20758:28262 147 MtDNA 9991 60 100M = 9506 -585 GATATTCCTGGGTTAGAATTTGACTCTTATATAAAATCACTAGATCAATTAAGTTTAGGTGAACCACGTTTATTAGAAGTTGATAATCGTTGTGTTATTC DEDDDDBBB?;DFFFFFFFEHHEGHIHEGIJJJJIIGHHIGIJIGIJJIGJJJJGHJJIIHHBIGFGBJJJIHEIFJJJJJJJIJJIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2105:2690:5126 99 MtDNA 10115 60 100M = 10123 108 GTATTACATCTGCTGATGTTATTCATGCTTGGGCATTAAATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTT C@@FFFFFHHHFHJJJJJJIIJJ>FHIIJJEHIIJIJJJJJJIJIJJJJJIFGGIIIJIIIJIIJJIJJIHHGGIFHIIIIHCAEHFHEFFEFFEDEC>@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2105:2690:5126 147 MtDNA 10123 60 100M = 10115 -108 TCTGCTGATGTTATTCATGCTTGGGCATTAAATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAG DEEDEDFFFFFFHHGFGHJGGIJHGDJIJJJJIGJJJIIIJHIIJJJJIGJJJIJJJJJJJIJJJJJIJJIJJJIHJJIJJJJJJJJHHHFBFFFFFCC@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:95:13542:11427/1 0 MtDNA 10154 60 101M * 0 0 ATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAGTGGGAGTTTTTTATGGTCAATGTTCAGAGATT GGGGDFGFDGEGGGGGFDGGGGGGGGEFGDGGDGFGEGFGFGGGGGGGDGFFEEGEFGBCGGGGGEGFBEEEECEBEEFFGE=EGEECDEEEEGGDCEDDB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:56:15270:6191/1 16 MtDNA 10392 60 101M * 0 0 ATATAGAGCTTTAAATTTTACTTGTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATA GEEEEGEEEEEDBGEFEGEEGGFEGGFGGEEFEGGGEGFGGDEGEGGEGCDFGGGGGGGGGGGGGGFAGGGGGGGGGGGGGGGGFDGGGGGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:51:14694:9582/1 16 MtDNA 10415 60 101M * 0 0 GTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATATTAGTTTTATTGTTTCATACTAA DEDEFEFEDAEEFF?EFFFEEBEAEE=ECCDDFFFFFFFFEFFEFFD=E@DFFFFFFFFFFFDBFFFEAFEFDFFFDEFFFEGGEGGFEGGGGGFFDFDDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:69:16487:9482/1 0 MtDNA 10798 60 101M * 0 0 TAGGCTTTATATTAAAGCTGGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTT GGGGGGEGGGDEGFGGGGGEGGFGGGGGGGGGGFGDGFGGDGGGEGGFGEGGGGDFEDFBFDEEEEGEFFGCDDFEEGEEEE=GGB@FFDFDGAGAA5EC5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:65:19066:9962/1 16 MtDNA 10886 60 101M * 0 0 CTTTTATTGAGTTCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAATTTAATTTTTATTTAAGAAAAAATAAATATATTTATAC E=@BBD@BEEEE@EE@EEEEEEEBEEBDDDE?FEEBEFFFDDBEBFEEFEDEEEFEBBEAEEFDADFF8CCEEE=??CCCCAFFBFCBEDCB=DEEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:57:11714:20002/1 0 MtDNA 11269 60 101M * 0 0 TTTATCTTGATAAATATTTATATTTAATACATTTAGTACGAAAGGAACATTGTAAAAGTTTTAAACTTTAAAGATTTTGAAATCTTTATTTTAGTGCTATT FFFFFEFFFAFFF?FFFFFFFFFFFDFFFFFEFFFFEFFFFDFFBDEFFDFDFFFFFFFFFFFEBFFFFDF=FFFFFFCEEEEFEFFFEFEEE=@BECBBD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:39:7418:8959/1 0 MtDNA 11394 60 101M * 0 0 TTTATTATTTGCTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAA GGGDGG?GGFFGGEGFFDFEFFFAFFFEFFFFFEFFFFFDEFFFDFFFF:E?EEEFACFFFFEEEFFFFAFFBFBFFBFFEBEC=ECEEBBBA=D=?CDAF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:12959:4517/1 16 MtDNA 11405 60 101M * 0 0 CTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAAAAATATGATTT EGGEFGGEBGFDGGGGG@GFGGGFEGGGFGGGDGGGGGGDFGGGGGFEGBGFFFD?GFGFEGGGEGGGGEGGGGGGFGDGFGGGGGGGGEGGFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:94:12001:2695/1 0 MtDNA 11494 60 101M * 0 0 AAAATTCAAAATTCTTTTAGAATTCATTTTTTTATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGA ?EEDEAEEEEDFEFFDEFEDFFEAFDFFFFFFCFDFCFEFF?FFFFFFFAFFDFAFFDFFFFFDFFFDFFFFF=F?DFEFFFA@FF?EFFF?FFEFFF=?: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:26:8245:3800/1 0 MtDNA 11527 60 101M * 0 0 ATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGATTTAAGTTCGTATATCAGGTTTTTAATAATATT :FGEEFGFFGGEGGGEFFFFDFFFFGFEGBFFEFEEEEEEDE?EEDEEEBAE?EAEEEECFEDFF=FBFEEA??EEEEEEFEEG?DFF?DEFF?AB;EDDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:70:14381:7815/1 0 MtDNA 11624 60 101M * 0 0 TATTCATCTTCATCTTGGGAGGATTTTACATAGAGTGATGATATGGTAAATTAGTTTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTT GGGGGEGGGGGGFGGEGGGGFGFGGGGAGGGFGGGEGFGFGFEGFGEG?GGGFGFFFFBF@EEDEGEEGBADGFGGGGGGGBGGGDGC@F?EECDFFFDAC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:9505:15324/1 16 MtDNA 11679 60 101M * 0 0 TTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTT EBE=E=EEEEEEEEEE=BEEDEEEEEDEEB?EEEEEEE>EEECCC;EEEEEEEDEEEEE5EDEEEEDDAEEEDDEEDADDD:EEDEDF?AFEBDEDDDBD? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:1:16170:13381/1 0 MtDNA 11707 60 101M * 0 0 TTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTTTTTTTTAGAATGAGATTTTTTAAGGTTA FFFFFBFFFFFDFDFGEGGGDG>@GAED=EDCCDA:DADCE?EEEFD==FFFEDB?FFFFC:EC:ECCBBC?EEECAFC8=C:=D?E5ECFEFDB:E:CAE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:85:11274:20902/1 0 MtDNA 11790 60 101M * 0 0 TGAGATTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTAGTTA GGGGFGGGGGGGGGGDGGGFGGGGGFGGFGGGGGGGGGGGGEGGGGFEGGGGGGGGGEGGGGGGGGGGEFCEFFGFFEEEEGEGGGCFDEGFEEF=:6??: PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:45:6186:13482/1 0 MtDNA 11795 60 101M * 0 0 TTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTACTTATTATT DFFFEBAEEEDDADDEDED==CCCCE=EEEEEEDBEEE?EEDDEEAB@EEDDEEDEEAEECEECA>:ACC@DCCB?DAABEBEBCEEEB?EBBEEB=E@EE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:26:5376:8009/1 0 MtDNA 11861 60 101M * 0 0 AACGTTTAGAGTTTTAGTTTTTAGTACTTATTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTA ADC<DCEDBEAF?D:DBEEAFBBDBAE?EEDG?GEEGEEFEE??ED:AD?EEEEEBBGGD?FADFDGGGFEBDEDDEAEEDGFGBE5CEE=DD=?:B>ABB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927 99 MtDNA 11891 60 100M = 11969 178 TTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACA CCCFFFFFHHHHFHIJJJIJGIJJJJIJJJJJJJJJJJJJJIJJJJIJJJJJJJJJHIJIFGIIJJJIJJJIJIJHGIJJJJJIIHHHHHADFFFFFDCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346 99 MtDNA 11949 60 100M = 12056 207 GGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATA BCBDFFFFHHHHHJJJJJIJJJJJIIIJJJJJCHIIJJJIIIJJJGIIJJJJIIJIGIFHHIHIJIJIFHHJJJJEIJEHIJHFDDDDDDEDDDDEEEDD PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:74:13913:12442/1 16 MtDNA 11959 60 101M * 0 0 TTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGAT GGGEEEFFBEEDGFGGFEDGFGGFGDGGGEGGGGGGGEGGFGFGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGFGGCAAA@D9EEEGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927 147 MtDNA 11969 60 100M = 11891 -178 TTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGATAGATGTAGG ADEEDDDCCEDDDDDDEEEEDEEEFFFFFFFHHHHHHJJJJJJJJJJJIIJJJIJJJJIIIJJJJJJIJJJJJJJJJJJIIIJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:27:3003:10682/1 16 MtDNA 12043 60 101M * 0 0 TTTATAATAATTGAGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTGTCGGTTTTT FB?CEACEE=EDEEE==?EE=DFFEAEEEE;FCEFEBAF?F?FFDFBEDGGFBEGGGGFGGGGEGFF?;FFFFDDDGDEGEFGEGGGGDAFGGGFGDFGGB PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346 147 MtDNA 12056 60 100M = 11949 -207 AGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTATCGGTTTTTAGAGGTTATTAT DDEDDDDDDDDDDDDDDDDFEFEDCDDDDDEEDCCEDFFFHHHHJJJJJJJJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:18352:37917 99 MtDNA 12193 60 100M = 12359 266 TATTATTACTTTTAACAGCTTTTACTAAAAGAGCACAATTTCCATTTAGATCTTGGTTACCCAAAGCTATAAGAGCCCCCACACCGGTGAGGTCTTTGGT @@@FFFDDHGHHHII@FGGGGGGIIIIIIJJIIGGIIJIJFHIGGIJFEHDBGHHGBGIJIHGIAEHIIIJCG@ACCDHEB<?A=>@,8<995<CCDD?B PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:14435:43021 99 MtDNA 12276 60 100M = 12492 316 CCGGTGAGGTCTTTGGTTCATAGTAGAACTTTAGTTACAGCAGGATTAATTCTATTAATAAATTTTAATAATTTAGTAATACAGAAAGATTTTATCAGTT B@@DADBDFCFHHIGGIIJGIJJJJJJJJJJJGIHGIGHJJIIJJJGHIIFFFICIIJIJICHJGGGFHHIIJIIIHIIJIHGGEHGF@BCFFECCDE@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:18352:37917 147 MtDNA 12359 60 100M = 12193 -266 GAAAGATTTTATCAGTTTTGTTCTGATTATTGGCCTATTTACTATATTTTTTTCTAGCTTAGCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCC DDDDCEDDDCCADCDDDDCAC>CACA@C>FFFFHGHHHEE@>CDAHFEGIEIJIGIGHIIGIGHGJIIIHIGIHIJGGHDJJIIHEIHHHHHFDFDF@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:4:6493:13587/1 0 MtDNA 12373 60 101M * 0 0 GTTTTGTTCTGATTATTGGCCTATTTACTATATTTTTTTCTAGCTTAGCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCA B88:7D??>BGFGDFEDDEFEDBEEEGEEBE=DEEEEEEEEEEGED@EDE5BABBEE>AAA?@?@@?5??BB?BBABA>B?7:??AAABAAAA?A<:>A>? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:5:16925:17939/1 0 MtDNA 12403 60 101M * 0 0 TATTTTTTTCTAGCTTAGCACGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGC FFF5FFEEECGGGEGEDEEE=EEEEEEEEEEEDEEEEEEEEEEEEEEDDEEE=E?BEEEEEEDEEEEEEEEEEEE6CCB>AEEEEEE?BADEEBEEAE=EE PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:79:6338:4546/1 0 MtDNA 12420 60 101M * 0 0 GCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTAT FFDFFFFFFDEFBFFGGGGGFGEEGGEFFFECEECFFFFFGGGGFGGGGGGGGGFGEFEGGGEGEFFF?DFDFCFEEFEFEBFGEEEEBGEEEEDGEEEEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798 99 MtDNA 12449 60 100M = 12482 133 GGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCT BCBDFDFFHHHHHJIJIJJJJJJJJJJJIJJIJEFHJJJGIGHJIIJJIJJJIIGHJIIIJJJJJJIEHHIGHHHFHHHFFFFFFFFEEEEEEEDCDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798 147 MtDNA 12482 60 100M = 12449 -133 TTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGT ADDDDDEECEC>CDFFFDHHHHIJJJJJIIJJJJJJJJJJJIJJJJJJJJJJJJIJJIJIIJJJIJJJIIGIJJJJJJJJJJJJJJJHHGHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:14435:43021 147 MtDNA 12492 60 100M = 12276 -316 GTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTAATCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTC ACC@CEDCDBDDFFHHHFIGHGIIGIJGIIIJHGHD?4JJJJIHIJJIGIHJIJJJJJIJIIJJJJIJJJJJJJJJJJJIHJJJIJJHHHHHFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947 99 MtDNA 12495 60 100M = 12781 386 ACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATT ?????DD::C:2<3C?;EECFFEIEFIEDE9?@E@DA<DDCDCDEEDAECDDDDA@DEDCEAC>C;CA7=ACCEDCEIEED>)=?A7?7?DDD@>BD@AD PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:102:10210:8668/1 0 MtDNA 12538 60 101M * 0 0 TTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATTGTTCATTTGGACAACAAGATGGACGTAATTATAGGAATAATGGT GFGGGGGGGG:FF?FGGGFGGGFGEBFGGGGFFDDGGGGGGGGAGDEEEEGFFBGGDED;EAGGGDGEEG?FDFFBEG5EEDCEEFDA5FEEBEF?EEB:: PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578 99 MtDNA 12620 60 100M = 12676 156 TAAGTATAGGAATAATGTTAATTTACCAAATTTTATTCAGTTACAAATATTAGTAACCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTA @CC1BDFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJJJJIGHJGIIIGHIFGIJJJJIGHHHFDBABCDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578 147 MtDNA 12676 60 100M = 12620 -156 CCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAG ?CCDDBDDDDDDDDDDDDDEDDEDEEEAFFFFFHHIIEJJJGIJJJJIHFGJJIJJIIGIJJJJIIJJJIJJJJJJJJJIJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:101:7524:6107/1 0 MtDNA 12685 60 101M * 0 0 GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT BEEEEDD5DDEEEDEFEDFFAFBDFFFFDFFFFAFEED=E:CBEEFFEDFFEEFEDFFFFDFFFFDF@EFBDFFBEFEFABCD:EFFCCCDD:EADFEFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:73:3706:15683/1 0 MtDNA 12685 60 101M * 0 0 GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT DDDD:DDBDBBEEEEGFFFGGGFGGGFGDF?ADCEFF=EB?CE:BFF=FCCE>FDEDEAAGAGCCEEGEEGGFEFEFEGD@EFADEFCC>E?E=BBBAEBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:35:14999:3587/1 0 MtDNA 12745 60 101M * 0 0 TTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTC DEFFFFFFFFBFD?FFFBFFFFFAFEEBECFDFFFFFDAFFFFFFCFFFBECECEFFEC?FBFFFDDEE??CDBEFFFFEEFFFFABFFFE8CFFFFFFE; PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:18737:28666 99 MtDNA 12755 60 100M = 12783 128 TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAAT CCCFFFFEHHHHHJJJJJJGJGHJJJIGIIJJJJJHIJIIIGHIJJJJJHIIJJJJJIJJJJJIGHHHHFFFFEECECEDDBDDDDDDDDBCCDCCDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:53:16100:4061/1 0 MtDNA 12755 60 101M * 0 0 TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATA FFDFFDEFDFAFE=FDFE@AFB?DFFF5FF?D@<@ACAEEE:ECEEEAA=FFDFDFADDFEEE@EEEEE@AABBB=AEEEDCCFDBEFE@@@DBADEDBBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947 147 MtDNA 12781 60 100M = 12495 -386 TATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCAC :?BBBB?CC@5CCCA9'=>>5(;>C;;A>>?;B@BFEFHEBG@=@>FB;BCGB?<GDEDC9<F<EEA<4D>HEGJHE<C<?<?A<2,,?B=2A4DDD8@? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:18737:28666 147 MtDNA 12783 60 100M = 12755 -128 TTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCACAG BDBDDDDCCDDDDBBDDDDEDCDDDDEEEFFFFFFHHFIJJJJJJJJIIHJIIJJJJJJJHGJJJJIJIJJIJJJJIJJJJJJJJJJGHHHHFFFDFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:109:6839:9395/1 16 MtDNA 12786 60 101M * 0 0 TTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCACAGTATT GGGGGFEFFGGGGGGDGGDGFDGGGGGGFGGGGGGFFGGGFGGGGGGGGGGGGGGGGGGFBGGGGGDGGGGGGGGGGGGGGFGGGGGGGGEGGFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:89:16184:15896/1 16 MtDNA 12850 60 101M * 0 0 TTAATAAAGTAATAAATCATTACAGTAGCACAGTATTTATAAATTTTTTAAGTTTAGTATTAGTTATTTTTTCTATTAGATTTTTATGATGAATAAATTTT EEEEFEEDFEEEGEBEEGEEBEGEEFGEGEGDGBFGFGGFGDFGEGGFEGGGFGEGGGGGFFGCEGGGGGGDGFFGDEGDGGGEGFDGGGGEGFGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:73:18789:5004/1 0 MtDNA 12862 60 97M1I3M * 0 0 TAAATCATTACAGTAGCACAGTATTTATAAATTTTTTAAGTTTAGTATTAGTTATTTTTTCTATTAGATTTTTATGATGAATAAATTTTAATCTAATAGAA GFEGFGGF=FEADEEEEEEDEEDEEFFFFFGGGGGGDGGGEGDFABE:EEBA=BBFFCCCGE@FF?BBBCACCB8>BEEEFFFGGFFGGE?EEEBD5?BBE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1114:11635:22812 99 MtDNA 12940 60 100M = 13083 243 GAATAAATTTTAATCTTCTTAACATCCCAAGACTTTTCTTATACGTAGATTTTTTTGGTCCTCTAGTATTTTTATTTATAATAATTTTTTTATCTTTTTT CCCFFFFFHHHHHJJJJIJJJJJJJIJJJJJJJJJJJJJJJJIIJHIJIJGHJJJJJIHHIJJJFHEHHHHHFFFFFFFEEFEEEFEDDDDDDDDDDDD< PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:50:13913:15312/1 16 MtDNA 13005 60 101M * 0 0 GTATTTTTATTTATAATAATTTTTTTATCTTTTTTAATTTTAAAAATATTATTTAAGGAGTTAATATACAAGTTTTTAGTTGATTATTTGGCTAAAAATAG ?>:>;::BCBB::FFF@@8<96BABC=@DFFBF=EDFDEFCAE=?EE?FAFEEC?=EEEC=FDEEBEEDAFF?EFFEBEDDEEEEBCDDD?E@<DDDEECE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1114:11635:22812 147 MtDNA 13083 60 100M = 12940 -243 GTTGATTATTTGGCTAAAAATAGTATTTATAAAATAAAGAATTTAAAATTTATAGATTTATTTTTAAATAATATTAATTCTAAGGGGTACACCTTATTTT @CFEDEFFFHGHGIJJJJJJJIHEGJJIHCJJJJJJJJJJJJJJJJJJJJJJJIJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHDHFFFFFCC@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:12001:40456 99 MtDNA 13159 60 100M = 13242 183 ATTCTAAGGGGTACACCTTATTTTTAAGCAGTGGTATATTTAAAAATTACTATTTAAAAAGGTTAAATTTTAATAGTGTAGTAGTTTTAATTTTTATTTT @@@FFFFDFFHDFGGEB;AB<EHGGGBH@FH?CC?F@GGIII>DGHGEDBDHHEDHGHIIGCHGI@@FGHIHGGIC==EC>)=A?CD>DDDEEECAACDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:12001:40456 147 MtDNA 13242 60 100M = 13159 -183 GTTTTAATTTTTATTTTTTTTATAATTTGTTAAGGGATTTTAGTTTAATAAAAATATATGTTTTGCATACATAAGATAATAATTCTAGATAGTTTTACAC @DDDDDDDCACDDDFGJIIJIJIIG@HIIJIHIHJJIIGJJIHGGIEIIGIIIIJIGIJJJJIGGCIIIGJIJJJIJJJIHIIJJJJHHFDGFFDDD@@@ PG:Z:SNAP RG:Z:000 NM:i:0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.vcf Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,192 @@ +##fileformat=VCFv4.2 +##FILTER=<ID=PASS,Description="All filters passed"> +##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat +##contig=<ID=chrI,length=25050> +##contig=<ID=chrII,length=25050> +##contig=<ID=chrIII,length=25050> +##contig=<ID=chrIV,length=25050> +##contig=<ID=chrV,length=25050> +##contig=<ID=chrX,length=25050> +##contig=<ID=MtDNA,length=13794> +##ALT=<ID=X,Description="Represents allele(s) other than observed."> +##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL."> +##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel"> +##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel"> +##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth"> +##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3"> +##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)"> +##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)"> +##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)"> +##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)"> +##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric."> +##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)"> +##INFO=<ID=ICB,Number=1,Type=Float,Description="Inbreeding Coefficient Binomial test (bigger is better)"> +##INFO=<ID=HOB,Number=1,Type=Float,Description="Bias in the number of HOMs number (smaller is better)"> +##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele, in the same order as listed"> +##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes"> +##INFO=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-forward , ref-reverse, alt-forward and alt-reverse bases"> +##INFO=<ID=MQ,Number=1,Type=Integer,Description="Average mapping quality"> +##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods"> +##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases"> +##FORMAT=<ID=DPR,Number=R,Type=Integer,Description="Number of high-quality bases observed for each allele"> +##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype"> +##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Phred-scaled Genotype Quality"> +##source=MiModD varcall (version 0.1.8.0) +##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet +##samtoolsCommand=n.a. (wrapped by MiModD) +##bcftools_callCommand=n.a. (wrapped by MiModD) +##rginfo=<ID=0,Rgid="000",Name="N2"> +##rginfo=<ID=1,Rgid="266-1",Name="ot266",Description="mutant strain carrying the vab-3 allele ot266"> +##samtoolsVersion=1.2+htslib-1.2.1 +##bcftools_callVersion=1.2+htslib-1.2.1 +##bcftools_concatVersion=1.2+htslib-1.2.1 +##bcftools_viewVersion=1.2+htslib-1.2.1 +##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT N2 ot266 +chrI 855 . G C 231.0 . DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,72,0:24:0,24:86 +chrI 885 . t tTCT 69.0 . INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,60:1:1,0:11 0/1:105,0,255:36:32,4:96 +chrI 912 . A T 195.0 . DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60 GT:PL:DP:DPR:GQ 0/1:26,0,54:2:1,1:25 0/1:204,0,255:88:67,21:127 +chrI 921 . G A 341.0 . DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0:2:0,2:25 1/1:255,239,0:92:1,91:127 +chrI 942 . A T 182.0 . DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60 GT:PL:DP:DPR:GQ 0/1:20,0,116:4:3,1:17 0/1:198,0,255:137:102,35:127 +chrI 945 . G A 396.0 . DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60 GT:PL:DP:DPR:GQ 1/1:177,12,0:4:0,4:19 0/1:255,0,86:134:22,112:78 +chrI 962 . G T 379.0 . DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60 GT:PL:DP:DPR:GQ 1/1:148,12,0:4:0,4:36 1/1:255,255,0:129:0,129:127 +chrI 973 . A G,T 329.0 . DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0,110,6,110:2:0,2,0:14 0/1:255,0,65,255,255,255:132:19,112,1:56 +chrI 974 . A T 9.21175 . DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:14 0/1:45,0,255:132:114,18:36 +chrI 998 . A T 99.0 . DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:134,0,255:89:71,18:127 +chrI 1000 . A T 93.0 . DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:13 0/1:128,0,255:99:80,19:120 +chrI 1028 . G A 105.0 . DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:140,0,255:57:46,11:127 +chrI 1041 . C T 6.52941 . DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,71:2:2,0:14 0/1:42,0,255:37:32,5:33 +chrI 1045 . T A 273.0 . DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,6,0:2:0,2:13 0/1:255,0,51:32:5,27:43 +chrI 1048 . G A 266.0 . DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:20 1/1:255,81,0:27:0,27:98 +chrI 1053 . c cT 8.64418 . INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:16 +chrI 1054 . A T 231.0 . DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:54 +chrI 1412 . T C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1414 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1421 . G A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 2752 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 13851 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13856 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13874 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13876 . C T 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 13901 . C A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13919 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrI 13921 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13944 . T A 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrI 13952 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13958 . C T 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:4 +chrI 13970 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13993 . A G 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 14201 . G A 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14223 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14242 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14246 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14247 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 22325 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 23943 . g gG 41.4034 . INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:66,6,0:2:0,2:9 +chrII 239 . A G 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrII 252 . A T 44.4523 . DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:75,0,160:10:7,3:74 +chrII 265 . A G 161.0 . DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:191,0,96:14:5,9:96 +chrII 278 . A T 167.0 . DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:197,0,111:14:5,9:111 +chrII 279 . A T,G 38.2143 . DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 0/1:69,0,186,71,175,247:14:9,4,1:67 +chrII 291 . A G 86.0 . DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:116,0,189:14:9,5:116 +chrII 300 . A C 231.0 . DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:53 +chrII 304 . A T 137.0 . DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:167,0,107:14:6,8:108 +chrII 305 . A T 27.2811 . DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:59,0,236:14:11,3:56 +chrII 317 . A G 101.0 . DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:131,0,172:14:8,6:127 +chrII 331 . A T 121.0 . DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:151,0,154:14:7,7:127 +chrII 343 . A G 90.0 . DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:120,0,74:9:3,6:74 +chrII 11921 . A T 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrII 17381 . G A 4.14838 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,34:2:1,1:32 ./.:0,0,0:0:0,0:0 +chrII 17518 . A T 14.9987 . DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:45,0,29:3:1,2:30 ./.:0,0,0:0:0,0:0 +chrII 19959 . T A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrIII 7994 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIII 8004 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrIII 8023 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8026 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8027 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8039 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9659 . C T 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9662 . G T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 9700 . A T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 9450 . A G 4.78389 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:33,0,27:2:1,1:28 ./.:0,0,0:0:0,0:0 +chrIV 16467 . T C 4.14182 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,25:2:1,1:26 ./.:0,0,0:0:0,0:0 +chrIV 16478 . C G 4.58205 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:35,0,23:2:1,1:28 0/0:0,3,29:1:1,0:5 +chrIV 16499 . A G 3.42278 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,79:2:2,0:4 0/1:35,3,0:1:0,1:3 +chrIV 16537 . aaga a 25.8002 . INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,120:2:2,0:4 0/1:60,3,0:1:0,1:3 +chrIV 16633 . C A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 16697 . C T 52.0 . DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:9 1/1:36,3,0:1:0,1:9 +chrIV 16709 . C G 5.30774 . DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,39:1:1,0:3 1/1:38,6,0:2:0,2:4 +chrIV 16729 . C T 4.14943 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,37:1:1,0:3 0/1:35,3,0:1:0,1:3 +chrIV 16769 . A T 13.7861 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:6 +chrIV 16814 . A G 3.03341 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,31:2:1,1:30 +chrIV 16815 . C A 48.4033 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:73,6,0:2:0,2:9 +chrIV 16823 . A C 4.14995 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,28:2:1,1:29 +chrIV 16829 . T C 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrIV 16833 . A T,G 14.5409 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:65,34,31,34,0,31:2:0,1,1:29 +chrIV 16834 . T A 3.56452 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,27:2:1,1:28 +chrIV 16894 . T C 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16899 . T A 49.5769 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:33,3,0:1:0,1:9 +chrIV 16902 . T G 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16921 . A G 68.0 . DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,3,0:1:0,1:11 1/1:38,3,0:1:0,1:11 +chrIV 16951 . C A 76.0 . DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:11 1/1:65,6,0:2:0,2:13 +chrIV 17000 . T C 149.0 . DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:14 1/1:132,18,0:6:0,6:29 +chrIV 17020 . C G,A 99.0 . DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,34,3,34,34:1:1,0,0:3 1/2:150,33,15,125,0,122:7:0,6,1:10 +chrIV 17024 . T C 14.4249 . DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:45,0,103:7:5,2:44 +chrIV 17037 . T A 37.8983 . DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:68,0,95:7:4,3:69 +chrIV 17051 . C T 103.0 . DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:127,15,0:5:0,5:22 +chrIV 17112 . T A 3.03494 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,30:2:1,1:30 +chrV 5001 . T G 4.94666 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:28,3,0:1:0,1:4 +chrV 10045 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10095 . T A 50.0 . DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:75,6,0:2:0,2:9 +chrV 10108 . C A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 10158 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrV 10166 . G A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10205 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10229 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10249 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10250 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10251 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10257 . A T 6.39934 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:30,3,0:1:0,1:4 +chrV 10279 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 14271 . T C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14273 . G C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14285 . T C 76.0 . DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:100,9,0:3:0,3:13 +chrV 14335 . T G 19.5632 . DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:50,0,98:6:4,2:49 +chrV 14337 . A G 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,14:6:1,5:9 +chrV 14339 . A G 154.0 . DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:178,18,0:6:0,6:25 +chrV 14346 . C T 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,18:6:1,5:14 +chrV 14367 . C A 104.0 . DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:128,12,0:4:0,4:19 +chrV 14445 . A G 21.4343 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:46,6,0:2:0,2:9 +chrV 14481 . G A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14488 . G T 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14528 . G A 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrV 14530 . A G 4.13076 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,22:2:1,1:23 +chrV 14538 . T C 32.4055 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:57,6,0:2:0,2:9 +chrX 1592 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1622 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1648 . A C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 1650 . T A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrX 6015 . A T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:38,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6032 . G A 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:32,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6058 . ta t 15.3937 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6118 . A G 15.394 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6143 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:37,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6984 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 7025 . A G 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 10527 . c cC 8.86989 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:33,3,0:1:0,1:4 +chrX 10535 . T C 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrX 10562 . C G 18.2649 . DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:49,0,131:7:5,2:48 +chrX 10595 . T G 148.0 . DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:172,21,0:7:0,7:29 +chrX 10629 . gt g 45.8065 . INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:70,6,0:2:0,2:13 +chrX 10630 . T C,G 10.4616 . DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:58,29,26,32,0,29:2:0,1,1:25 +chrX 10643 . G T 34.5919 . DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:59,6,0:2:0,2:11 +chrX 15480 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 15495 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24890 . C G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24935 . A C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +MtDNA 954 . G T 4.79534 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/1:36,3,0:1:0,1:3 0/1:0,3,33:1:1,0:3 +MtDNA 5979 . C A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:31,3,0:1:0,1:4 +MtDNA 8429 . A G 38.5774 . DP=2;VDB=0.36;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:26,3,0:1:0,1:9 1/1:37,3,0:1:0,1:9 +MtDNA 12134 . A G 6.22224 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,41:1:1,0:3 0/1:38,3,0:1:0,1:3 +MtDNA 12637 . G T 8.72328 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:41,3,0:1:0,1:3 0/1:0,3,25:1:1,0:3 +MtDNA 12998 . C T 14.4264 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:39,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ce11ToCe10.over.chain Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,871 @@ +chain 1420037770 chrI 15072434 + 0 15072434 chrI 15072423 + 0 15072423 5 +288367 0 1 +49582 0 1 +3348 0 1 +884584 1 0 +731267 0 1 +203378 1 0 +1324625 0 1 +399852 13 13 +122370 1 0 +213502 1 0 +782497 1 0 +39234 0 3 +867656 1 0 +595979 1 0 +295566 2 0 +147478 1 1 +23 1 1 +191487 13 13 +8983 1 0 +213892 1 0 +58238 1 0 +125173 11 11 +206247 1 0 +126100 1 1 +46 1 1 +281857 1 0 +118832 1 0 +67661 0 1 +470697 1 0 +457535 1 0 +70047 4 4 +38252 0 1 +84452 1 0 +164033 1 0 +319488 1 0 +78543 1 0 +82353 0 1 +129773 0 1 +246657 1 0 +5362 1 0 +354010 0 4 +296600 1 0 +240963 1 0 +37688 1 0 +81200 0 1 +883251 1 0 +528183 1 0 +98784 1 1 +39 1 1 +65027 1 0 +20874 0 1 +542549 1 0 +216555 1 1 +30 1 1 +489109 1 0 +27002 0 1 +222382 0 1 +473092 + +chain 1440101603 chrII 15279421 + 0 15279421 chrII 15279345 + 0 15279345 4 +523340 1 0 +72281 0 8 +1033090 1 0 +219329 1 0 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/header_only.sam Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,2 @@ +@HD VN:1.5 +@RG ID:000 SM:N2 DS:C. elegans wt CN:ABC DT:2017-06-27 LB:XYZ PI:400 PL:ILLUMINA PU:SEQ123
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/rebased.vcf Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,186 @@ +##fileformat=VCFv4.2 +##FILTER=<ID=PASS,Description="All filters passed"> +##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat +##contig=<ID=chrI,length=15072423> +##contig=<ID=chrII,length=15279345> +##contig=<ID=chrIII,length=13783700> +##contig=<ID=chrIV,length=17493793> +##contig=<ID=chrM,length=13794> +##contig=<ID=chrV,length=20924149> +##contig=<ID=chrX,length=17718866> +##ALT=<ID=X,Description="Represents allele(s) other than observed."> +##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL."> +##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel"> +##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel"> +##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth"> +##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3"> +##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)"> +##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)"> +##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)"> +##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)"> +##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric."> +##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)"> +##INFO=<ID=ICB,Number=1,Type=Float,Description="Inbreeding Coefficient Binomial test (bigger is better)"> +##INFO=<ID=HOB,Number=1,Type=Float,Description="Bias in the number of HOMs number (smaller is better)"> +##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele, in the same order as listed"> +##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes"> +##INFO=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-forward , ref-reverse, alt-forward and alt-reverse bases"> +##INFO=<ID=MQ,Number=1,Type=Integer,Description="Average mapping quality"> +##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods"> +##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases"> +##FORMAT=<ID=DPR,Number=R,Type=Integer,Description="Number of high-quality bases observed for each allele"> +##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype"> +##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Phred-scaled Genotype Quality"> +##source=MiModD varcall (version 0.1.8.0) +##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet +##samtoolsCommand=n.a. (wrapped by MiModD) +##bcftools_callCommand=n.a. (wrapped by MiModD) +##rginfo=<ID=0,Rgid="000",Name="N2"> +##rginfo=<ID=1,Rgid="266-1",Name="ot266",Description="mutant strain carrying the vab-3 allele ot266"> +##samtoolsVersion=1.2+htslib-1.2.1 +##bcftools_callVersion=1.2+htslib-1.2.1 +##bcftools_concatVersion=1.2+htslib-1.2.1 +##bcftools_viewVersion=1.2+htslib-1.2.1 +##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT N2 ot266 +chrI 855 . G C 231.0 . DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,72,0:24:0,24:86 +chrI 885 . t tTCT 69.0 . INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,60:1:1,0:11 0/1:105,0,255:36:32,4:96 +chrI 912 . A T 195.0 . DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60 GT:PL:DP:DPR:GQ 0/1:26,0,54:2:1,1:25 0/1:204,0,255:88:67,21:127 +chrI 921 . G A 341.0 . DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0:2:0,2:25 1/1:255,239,0:92:1,91:127 +chrI 942 . A T 182.0 . DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60 GT:PL:DP:DPR:GQ 0/1:20,0,116:4:3,1:17 0/1:198,0,255:137:102,35:127 +chrI 945 . G A 396.0 . DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60 GT:PL:DP:DPR:GQ 1/1:177,12,0:4:0,4:19 0/1:255,0,86:134:22,112:78 +chrI 962 . G T 379.0 . DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60 GT:PL:DP:DPR:GQ 1/1:148,12,0:4:0,4:36 1/1:255,255,0:129:0,129:127 +chrI 973 . A G,T 329.0 . DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0,110,6,110:2:0,2,0:14 0/1:255,0,65,255,255,255:132:19,112,1:56 +chrI 974 . A T 9.21175 . DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:14 0/1:45,0,255:132:114,18:36 +chrI 998 . A T 99.0 . DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:134,0,255:89:71,18:127 +chrI 1000 . A T 93.0 . DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:13 0/1:128,0,255:99:80,19:120 +chrI 1028 . G A 105.0 . DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:140,0,255:57:46,11:127 +chrI 1041 . C T 6.52941 . DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,71:2:2,0:14 0/1:42,0,255:37:32,5:33 +chrI 1045 . T A 273.0 . DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,6,0:2:0,2:13 0/1:255,0,51:32:5,27:43 +chrI 1048 . G A 266.0 . DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:20 1/1:255,81,0:27:0,27:98 +chrI 1053 . c cT 8.64418 . INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:16 +chrI 1054 . A T 231.0 . DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:54 +chrI 1412 . T C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1414 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1421 . G A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 2752 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 13851 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13856 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13874 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13876 . C T 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 13901 . C A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13919 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrI 13921 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13944 . T A 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrI 13952 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13958 . C T 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:4 +chrI 13970 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13993 . A G 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 14201 . G A 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14223 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14242 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14246 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14247 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 22325 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 23943 . g gG 41.4034 . INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:66,6,0:2:0,2:9 +chrII 239 . A G 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrII 252 . A T 44.4523 . DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:75,0,160:10:7,3:74 +chrII 265 . A G 161.0 . DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:191,0,96:14:5,9:96 +chrII 278 . A T 167.0 . DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:197,0,111:14:5,9:111 +chrII 279 . A T,G 38.2143 . DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 0/1:69,0,186,71,175,247:14:9,4,1:67 +chrII 291 . A G 86.0 . DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:116,0,189:14:9,5:116 +chrII 300 . A C 231.0 . DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:53 +chrII 304 . A T 137.0 . DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:167,0,107:14:6,8:108 +chrII 305 . A T 27.2811 . DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:59,0,236:14:11,3:56 +chrII 317 . A G 101.0 . DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:131,0,172:14:8,6:127 +chrII 331 . A T 121.0 . DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:151,0,154:14:7,7:127 +chrII 343 . A G 90.0 . DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:120,0,74:9:3,6:74 +chrII 11921 . A T 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrII 17381 . G A 4.14838 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,34:2:1,1:32 ./.:0,0,0:0:0,0:0 +chrII 17518 . A T 14.9987 . DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:45,0,29:3:1,2:30 ./.:0,0,0:0:0,0:0 +chrII 19959 . T A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrIII 7994 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIII 8004 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrIII 8023 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8026 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8027 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8039 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9659 . C T 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9662 . G T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 9700 . A T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 9450 . A G 4.78389 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:33,0,27:2:1,1:28 ./.:0,0,0:0:0,0:0 +chrIV 16467 . T C 4.14182 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,25:2:1,1:26 ./.:0,0,0:0:0,0:0 +chrIV 16478 . C G 4.58205 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:35,0,23:2:1,1:28 0/0:0,3,29:1:1,0:5 +chrIV 16499 . A G 3.42278 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,79:2:2,0:4 0/1:35,3,0:1:0,1:3 +chrIV 16537 . aaga a 25.8002 . INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,120:2:2,0:4 0/1:60,3,0:1:0,1:3 +chrIV 16633 . C A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 16697 . C T 52.0 . DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:9 1/1:36,3,0:1:0,1:9 +chrIV 16709 . C G 5.30774 . DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,39:1:1,0:3 1/1:38,6,0:2:0,2:4 +chrIV 16729 . C T 4.14943 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,37:1:1,0:3 0/1:35,3,0:1:0,1:3 +chrIV 16769 . A T 13.7861 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:6 +chrIV 16814 . A G 3.03341 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,31:2:1,1:30 +chrIV 16815 . C A 48.4033 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:73,6,0:2:0,2:9 +chrIV 16823 . A C 4.14995 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,28:2:1,1:29 +chrIV 16829 . T C 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrIV 16833 . A T,G 14.5409 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:65,34,31,34,0,31:2:0,1,1:29 +chrIV 16834 . T A 3.56452 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,27:2:1,1:28 +chrIV 16894 . T C 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16899 . T A 49.5769 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:33,3,0:1:0,1:9 +chrIV 16902 . T G 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16921 . A G 68.0 . DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,3,0:1:0,1:11 1/1:38,3,0:1:0,1:11 +chrIV 16951 . C A 76.0 . DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:11 1/1:65,6,0:2:0,2:13 +chrIV 17000 . T C 149.0 . DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:14 1/1:132,18,0:6:0,6:29 +chrIV 17020 . C G,A 99.0 . DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,34,3,34,34:1:1,0,0:3 1/2:150,33,15,125,0,122:7:0,6,1:10 +chrIV 17024 . T C 14.4249 . DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:45,0,103:7:5,2:44 +chrIV 17037 . T A 37.8983 . DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:68,0,95:7:4,3:69 +chrIV 17051 . C T 103.0 . DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:127,15,0:5:0,5:22 +chrIV 17112 . T A 3.03494 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,30:2:1,1:30 +chrV 5001 . T G 4.94666 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:28,3,0:1:0,1:4 +chrV 10045 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10095 . T A 50.0 . DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:75,6,0:2:0,2:9 +chrV 10108 . C A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 10158 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrV 10166 . G A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10205 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10229 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10249 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10250 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10251 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10257 . A T 6.39934 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:30,3,0:1:0,1:4 +chrV 10279 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 14271 . T C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14273 . G C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14285 . T C 76.0 . DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:100,9,0:3:0,3:13 +chrV 14335 . T G 19.5632 . DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:50,0,98:6:4,2:49 +chrV 14337 . A G 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,14:6:1,5:9 +chrV 14339 . A G 154.0 . DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:178,18,0:6:0,6:25 +chrV 14346 . C T 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,18:6:1,5:14 +chrV 14367 . C A 104.0 . DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:128,12,0:4:0,4:19 +chrV 14445 . A G 21.4343 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:46,6,0:2:0,2:9 +chrV 14481 . G A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14488 . G T 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14528 . G A 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrV 14530 . A G 4.13076 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,22:2:1,1:23 +chrV 14538 . T C 32.4055 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:57,6,0:2:0,2:9 +chrX 1592 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1622 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1648 . A C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 1650 . T A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrX 6015 . A T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:38,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6032 . G A 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:32,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6058 . ta t 15.3937 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6118 . A G 15.394 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6143 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:37,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6984 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 7025 . A G 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 10527 . c cC 8.86989 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:33,3,0:1:0,1:4 +chrX 10535 . T C 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrX 10562 . C G 18.2649 . DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:49,0,131:7:5,2:48 +chrX 10595 . T G 148.0 . DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:172,21,0:7:0,7:29 +chrX 10629 . gt g 45.8065 . INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:70,6,0:2:0,2:13 +chrX 10630 . T C,G 10.4616 . DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:58,29,26,32,0,29:2:0,1,1:25 +chrX 10643 . G T 34.5919 . DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:59,6,0:2:0,2:11 +chrX 15480 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 15495 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24890 . C G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24935 . A C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/so_queryname.sam Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,2 @@ +@HD VN:1.5 SO:queryname +@RG ID:000 SM:N2 DS:C. elegans wt CN:ABC DT:2017-06-27 LB:XYZ PI:400 PL:ILLUMINA PU:SEQ123
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_pair_reads_1.fastqsanger Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,20 @@ +@HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 +GTCAATTGTACTGGTCAATACTAAAAGAATAGGATC ++HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:22:1621:462/1 +ATAATGGCTATTATTGTGGGGGGGATGATGCTGGAA ++HWI-EAS91_1_30788AAXX:7:22:1621:462/1 +hhhhhhhhhhhhQAhh@hhhhNhhhfhMbCIScC?h +@HWI-EAS91_1_30788AAXX:7:45:408:807/1 +TACCCGATTTTTTGCTTTCCACTTTATCCTACCCTT ++HWI-EAS91_1_30788AAXX:7:45:408:807/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:49:654:1439/1 +CTAACTCTATTTATTGTATTTCAACTAAAAATCTCA ++HWI-EAS91_1_30788AAXX:7:49:654:1439/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:64:947:234/1 +TATCAAAAAAGAATATAATCTGAATCAACACTACAA ++HWI-EAS91_1_30788AAXX:7:64:947:234/1 +hhhhhhhhhhhhhhhhhhhhhhhRhhehhahhhhhJ
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_pair_reads_2.fastqsanger Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,20 @@ +@HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 +GCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA ++HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 +hhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR +@HWI-EAS91_1_30788AAXX:7:22:1621:462/2 +ACTAGCCCCAATATCAATCCTATATCAAATCTCACC ++HWI-EAS91_1_30788AAXX:7:22:1621:462/2 +hJhhhhChhhJhhhRhhKhePhc\KhhV\KhXhJhh +@HWI-EAS91_1_30788AAXX:7:45:408:807/2 +ATGAGTGCTAGGATCAGGATGGAGAGGATTAGGGCT ++HWI-EAS91_1_30788AAXX:7:45:408:807/2 +hhhhhhhhhhhhhhhhhh`hhhZh`hhhhhRXhhYh +@HWI-EAS91_1_30788AAXX:7:49:654:1439/2 +TAGGTTTATTGATAGTTGTGTTGTTGGTGTAAATGG ++HWI-EAS91_1_30788AAXX:7:49:654:1439/2 +hhhhhhhhhhhhhhhhhhhhhhhhhdhh_hG\XhU@ +@HWI-EAS91_1_30788AAXX:7:64:947:234/2 +CCTATTAGTGTGTAGAATAGGAAGTAGAGGCCTGCG ++HWI-EAS91_1_30788AAXX:7:64:947:234/2 +hhhhhhhh^hPhWfhhhhThWUhhfhh_hhNIVPUd
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/all_fasta.loc.sample Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,18 @@ +#This file lists the locations and dbkeys of all the fasta files +#under the "genome" directory (a directory that contains a directory +#for each build). The script extract_fasta.py will generate the file +#all_fasta.loc. This file has the format (white space characters are +#TAB characters): +# +#<unique_build_id> <dbkey> <display_name> <file_path> +# +#So, all_fasta.loc could look something like this: +# +#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa +#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa +# +#Your all_fasta.loc file should contain an entry for each individual +#fasta file. So there will be multiple fasta files for each build, +#such as with hg19 above. +#
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Sat Nov 11 18:18:54 2017 -0500 @@ -0,0 +1,7 @@ +<tables> + <!-- Locations of all fasta files under genome directory --> + <table name="all_fasta" comment_char="#"> + <columns>value, dbkey, name, path</columns> + <file path="tool-data/all_fasta.loc" /> + </table> +</tables>