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1 #!/usr/bin/env python
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2 """
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3 Concatenate two bed files. The concatenated files are returned in the
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4 same format as the first. If --sameformat is specified, then all
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5 columns will be treated as the same, and all fields will be saved,
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6 although the output will be trimmed to match the primary input. In
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7 addition, if --sameformat is specified, missing fields will be padded
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8 with a period(.).
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9
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10 usage: %prog in_file_1 in_file_2 out_file
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11 -1, --cols1=N,N,N,N: Columns for chrom, start, end, strand in first file
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12 -2, --cols2=N,N,N,N: Columns for chrom, start, end, strand in second file
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13 -s, --sameformat: All files are precisely the same format.
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14 """
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15 from galaxy import eggs
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16 import pkg_resources
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17 pkg_resources.require( "bx-python" )
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18 import sys, traceback, fileinput
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19 from warnings import warn
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20 from bx.intervals import *
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21 from bx.intervals.io import *
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22 from bx.intervals.operations.concat import *
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23 from bx.cookbook import doc_optparse
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24 from galaxy.tools.util.galaxyops import *
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25
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26 assert sys.version_info[:2] >= ( 2, 4 )
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27
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28 def main():
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29 sameformat=False
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30 upstream_pad = 0
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31 downstream_pad = 0
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32
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33 options, args = doc_optparse.parse( __doc__ )
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34 try:
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35 chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols1 )
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36 chr_col_2, start_col_2, end_col_2, strand_col_2 = parse_cols_arg( options.cols2 )
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37 if options.sameformat: sameformat = True
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38 in_file_1, in_file_2, out_fname = args
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39 except:
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40 doc_optparse.exception()
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41
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42 g1 = NiceReaderWrapper( fileinput.FileInput( in_file_1 ),
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43 chrom_col=chr_col_1,
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44 start_col=start_col_1,
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45 end_col=end_col_1,
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46 fix_strand=True )
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47
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48 g2 = NiceReaderWrapper( fileinput.FileInput( in_file_2 ),
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49 chrom_col=chr_col_2,
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50 start_col=start_col_2,
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51 end_col=end_col_2,
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52 strand_col=strand_col_2,
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53 fix_strand=True )
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54
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55 if strand_col_1 >= 0:
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56 g1.strand_col = strand_col_1
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57
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58 out_file = open( out_fname, "w" )
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59
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60 try:
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61 for line in concat( [g1, g2], sameformat=sameformat ):
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62 if type( line ) is GenomicInterval:
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63 out_file.write( "%s\n" % "\t".join( line.fields ) )
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64 else:
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65 out_file.write( "%s\n" % line )
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66 except ParseError, exc:
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67 out_file.close()
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68 fail( "Invalid file format: %s" % str( exc ) )
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69
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70 out_file.close()
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71
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72 if g1.skipped > 0:
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73 print skipped( g1, filedesc=" of 1st dataset" )
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74 if g2.skipped > 0:
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75 print skipped( g2, filedesc=" of 2nd dataset" )
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76
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77 if __name__ == "__main__":
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78 main()
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