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1 <tool id="trinity_all" name="Trinity" version="0.0.1">
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2 <!-- Run all steps of Trinity-Inchworm, Chrysalis, and Butterfly-in a single step. Wrapper status is alpha. -->
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3 <description>De novo assembly of RNA-Seq data</description>
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4 <requirements>
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5 <requirement type="package">trinity</requirement>
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6 </requirements>
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7 <command>
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8 Trinity.pl
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9
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10 ## Additional parameters.
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11 #if $additional_params.use_additional == "yes":
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12 --min_contig_length $additional_params.min_contig_length
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13 #end if
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14
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15 ## Inputs.
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16 #if $inputs.paired_or_single == "paired":
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17 --left $inputs.left_input --right $inputs.right_input
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18 #if $inputs.left_input.ext == 'fa':
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19 --seqType fa
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20 #else:
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21 --seqType fq
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22 #end if
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23 #if $inputs.library_type != 'None':
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24 --SS_lib_type $inputs.library_type
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25 #end if
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26 #else:
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27 --single $inputs.input
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28 #if $inputs.input.ext == 'fa':
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29 --seqType fa
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30 #else:
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31 --seqType fq
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32 #end if
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33 #if $inputs.library_type != 'None':
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34 --SS_lib_type $inputs.library_type
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35 #end if
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36 #end if
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37
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38 ## CPU and butterfly options.
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39 --CPU 4 --run_butterfly --bfly_opts "-V 10 --stderr" > $trinity_log 2>&1
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40 </command>
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41 <inputs>
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42 <conditional name="inputs">
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43 <param name="paired_or_single" type="select" label="Paired or Single-end data?">
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44 <option value="paired">Paired</option>
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45 <option value="single">Single</option>
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46 </param>
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47 <when value="paired">
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48 <param format="fasta,fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/>
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49 <param format="fasta,fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/>
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50 <param name="library_type" type="select" label="Strand-specific Library Type">
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51 <option value="None">None</option>
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52 <option value="FR">FR</option>
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53 <option value="RF">RF</option>
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54 </param>
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55 <param name="paired_fragment_length" type="integer" value="300" min="1" label="Paired Fragment Length" help="Maximum length expected between fragment pairs"/>
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56 </when>
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57 <when value="single">
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58 <param format="fasta,fastq" name="input" type="data" label="Single-end reads" help=""/>
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59 <param name="library_type" type="select" label="Strand-specific Library Type">
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60 <option value="None">None</option>
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61 <option value="F">F</option>
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62 <option value="R">R</option>
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63 </param>
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64 </when>
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65 </conditional>
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66 <conditional name="additional_params">
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67 <param name="use_additional" type="select" label="Use Additional Params?">
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68 <option value="no">No</option>
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69 <option value="yes">Yes</option>
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70 </param>
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71 <when value="no">
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72 </when>
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73 <when value="yes">
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74 <param name="min_contig_length" type="integer" value="200" min="1" label="Minimum Contig Length" help=""/>
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75 </when>
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76 </conditional>
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77 </inputs>
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78 <outputs>
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79 <data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" />
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80 <data format="fasta" name="assembled_transcripts" label="${tool.name} on ${on_string}: Assembled Transcripts" from_work_dir="trinity_out_dir/Trinity.fasta"/>
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81 </outputs>
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82 <tests>
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83 </tests>
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84 <help>
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85 Trinity is a de novo transcript assembler that uses RNA-seq data as input. This tool runs all Trinity_ commands--Inchworm, Chrysalis, and Butterfly--in a single pass.
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86
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87 .. _Trinity: http://trinityrnaseq.sourceforge.net
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88 </help>
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89 </tool>
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