This tool uses the SAMtools toolkit to remove potential PCR duplicates: if multiple read pairs have identical external coordinates, only retain the pair with highest mapping quality. In the paired-end mode, this command ONLY works with FR orientation and requires ISIZE is correctly set. It does not work for unpaired reads (e.g. two ends mapped to different chromosomes or orphan reads). |
hg clone https://toolshed.g2.bx.psu.edu/repos/devteam/samtools_rmdup
Name | Description | Version | Minimum Galaxy Version |
---|---|---|---|
remove PCR duplicates | 2.0.1 | 16.01 |