Mercurial > repos > peterjc > seq_filter_by_id
annotate tools/seq_filter_by_id/README.rst @ 4:1c36cf8ef133 draft
Uploaded v0.0.7 fixed dependency on Biopython 1.62
author | peterjc |
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date | Wed, 30 Jul 2014 06:39:53 -0400 |
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1 Galaxy tool to filter FASTA, FASTQ or SFF sequences by ID |
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2 ========================================================= |
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3 |
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4 This tool is copyright 2010-2013 by Peter Cock, The James Hutton Institute |
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5 (formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. |
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6 See the licence text below. |
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7 |
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8 This tool is a short Python script (using both the Galaxy and Biopython library |
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9 functions) which divides a FASTA, FASTQ, or SFF file in two, those sequences with |
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10 or without an ID present in the specified column(s) of a tabular file. Example uses |
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11 include filtering based on search results from a tool like NCBI BLAST before |
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12 assembly. |
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13 |
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14 This tool is available from the Galaxy Tool Shed at: |
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15 |
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16 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id |
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17 |
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18 See also sister tools: |
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19 |
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20 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_select_by_id |
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21 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_rename |
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22 |
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23 |
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24 Automated Installation |
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25 ====================== |
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26 |
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27 This should be straightforward using the Galaxy Tool Shed, which should be |
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28 able to automatically install the dependency on Biopython, and then install |
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29 this tool and run its unit tests. |
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30 |
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31 |
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32 Manual Installation |
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33 =================== |
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34 |
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35 There are just two files to install to use this tool from within Galaxy: |
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36 |
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37 * seq_filter_by_id.py (the Python script) |
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38 * seq_filter_by_id.xml (the Galaxy tool definition) |
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39 |
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40 The suggested location is a dedicated tools/seq_filter_by_id folder. |
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41 |
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42 You will also need to modify the tools_conf.xml file to tell Galaxy to offer the |
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43 tool. One suggested location is in the filters section. Simply add the line:: |
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44 |
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45 <tool file="seq_filter_by_id/seq_filter_by_id.xml" /> |
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46 |
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47 If you wish to run the unit tests, also add this to tools_conf.xml.sample |
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48 and move/copy the test-data files under Galaxy's test-data folder. Then:: |
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49 |
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50 $ ./run_functional_tests.sh -id seq_filter_by_id |
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51 |
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52 You will also need to install Biopython 1.54 or later. That's it. |
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53 |
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54 |
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55 History |
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56 ======= |
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57 |
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58 ======= ====================================================================== |
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59 Version Changes |
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60 ------- ---------------------------------------------------------------------- |
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61 v0.0.1 - Initial version, combining three separate scripts for each file format. |
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62 v0.0.4 - Record script version when run from Galaxy. |
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63 - Faster FASTA code which preserves the original line wrapping. |
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64 - Basic unit test included. |
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65 v0.0.5 - Check for errors using Python script's return code. |
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66 - Cope with malformed FASTA entries without an identifier. |
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67 v0.0.6 - Link to Tool Shed added to help text and this documentation. |
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68 - Automated installation of the Biopython dependency. |
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69 - Use reStructuredText for this README file. |
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70 - Adopt standard MIT License. |
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71 - Updated citation information (Cock et al. 2013). |
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72 - Development moved to GitHub, https://github.com/peterjc/pico_galaxy |
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73 - Renamed folder and adopted README.rst naming. |
4 | 74 v0.0.7 - Correct automated dependency definition. |
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75 ======= ====================================================================== |
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76 |
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77 |
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78 |
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79 Developers |
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80 ========== |
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81 |
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82 This script and related tools were initially developed on the following hg branches: |
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83 http://bitbucket.org/peterjc/galaxy-central/src/fasta_filter |
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84 http://bitbucket.org/peterjc/galaxy-central/src/tools |
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85 |
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86 Development has now moved to a dedicated GitHub repository: |
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87 https://github.com/peterjc/pico_galaxy/tree/master/tools |
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88 |
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89 For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use |
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90 the following command from the Galaxy root folder:: |
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91 |
4 | 92 $ tar -czf seq_filter_by_id.tar.gz tools/seq_filter_by_id/README.rst tools/seq_filter_by_id/seq_filter_by_id.* tools/seq_filter_by_id/tool_dependencies.xml test-data/k12_ten_proteins.fasta test-data/k12_hypothetical.fasta test-data/k12_hypothetical.tabular |
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93 |
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94 Check this worked:: |
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95 |
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96 $ tar -tzf seq_filter_by_id.tar.gz |
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97 tools/seq_filter_by_id/README.rst |
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98 tools/seq_filter_by_id/seq_filter_by_id.py |
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99 tools/seq_filter_by_id/seq_filter_by_id.xml |
4 | 100 tools/seq_filter_by_id/tool_dependencies.xml |
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101 test-data/k12_ten_proteins.fasta |
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102 test-data/k12_hypothetical.fasta |
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103 test-data/k12_hypothetical.tabular |
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104 |
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105 |
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106 Licence (MIT) |
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107 ============= |
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108 |
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109 Permission is hereby granted, free of charge, to any person obtaining a copy |
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110 of this software and associated documentation files (the "Software"), to deal |
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111 in the Software without restriction, including without limitation the rights |
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112 to use, copy, modify, merge, publish, distribute, sublicense, and/or sell |
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113 copies of the Software, and to permit persons to whom the Software is |
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114 furnished to do so, subject to the following conditions: |
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115 |
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116 The above copyright notice and this permission notice shall be included in |
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117 all copies or substantial portions of the Software. |
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118 |
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119 THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR |
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120 IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, |
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121 FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE |
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122 AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER |
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123 LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, |
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124 OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN |
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125 THE SOFTWARE. |