Mercurial > repos > artbio > artbio_bam_cleaning

changeset 3:a1462dd52f17 draft

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"planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/artbio_bam_cleaning commit b022da54be013bf593ad96de6e7bd8b9e6bb5523"
author artbio
date Tue, 16 Feb 2021 00:19:57 +0000
parents c973ff00c785
children 15ada8e1580a
files artbio_bam_cleaning.xml
diffstat 1 files changed, 4 insertions(+), 21 deletions(-) [+]
line wrap: on
line diff
--- a/artbio_bam_cleaning.xml	Sun Feb 14 22:45:31 2021 +0000
+++ b/artbio_bam_cleaning.xml	Tue Feb 16 00:19:57 2021 +0000
@@ -1,4 +1,4 @@
-<tool id="artbio_bam_cleaning" name="ARTbio bam cleaning" version="1.6+galaxy2">
+<tool id="artbio_bam_cleaning" name="ARTbio bam cleaning" version="1.6+galaxy3">
     <description>
         on flags and PCR Duplicates and MD recalibration
     </description>
@@ -23,41 +23,24 @@
     | samtools rmdup - -
     |tee $input_base".filt1.dedup.bam"| bamleftalign --fasta-reference reference.fa -c --max-iterations "5" -
     | samtools calmd  -C 50 -b -@ \${GALAXY_SLOTS:-2} - reference.fa > $calmd
-    #if $pipeline == 'fullfilter':
     && sambamba view -h -t \${GALAXY_SLOTS:-2} --filter='mapping_quality <= 254' -f 'bam' -o $fullfilter $calmd
-    #end if
     ]]></command>
     <inputs>
         <expand macro="reference_source_conditional" />
         <param name="input_bam" type="data" format="bam" label="BAM or SAM file to process"/>
-        <param name="pipeline" type="select" label="where to stop the pipeline">
-            <option value="CalMD">At CalMD processing to keep split read alignments</option>
-            <option value="fullfilter" selected="true">Full bam processing, will eliminate split read alignments in the final bam file</option>
-        </param>
     </inputs>
     <outputs>
-        <data name="calmd" format="bam" label="CalMD filter (for lumpy-smoove)">
-            <filter>pipeline == 'CalMD'</filter>
-        </data>
-        <data name="fullfilter" format="bam" label="Full filtering (for somatic-varscan)">
-             <filter>pipeline == 'fullfilter'</filter>
-        </data>
-       
+        <data name="calmd" format="bam" label="CalMD filter (for lumpy-smoove)" />
+        <data name="fullfilter" format="bam" label="Full filtering (for somatic-varscan)" />
     </outputs>
     <tests>
         <test>
             <param name="input_bam" value="match_chr21_DBA_974.bam" ftype="bam" />
             <param name="reference_source_selector" value="history" />
             <param name="ref_file" value="chr21.fa" />
+            <output name="calmd" file="match_chr21_DBA_974.filt1.dedup.bamleft.calmd.bam" ftype="bam" />
             <output name="fullfilter" file="match_chr21_DBA_974.filt1.dedup.bamleft.calmd.filt2.bam" ftype="bam" />
         </test>
-        <test>
-            <param name="input_bam" value="match_chr21_DBA_974.bam" ftype="bam" />
-            <param name="reference_source_selector" value="history" />
-            <param name="ref_file" value="chr21.fa" />
-            <param name="pipeline" value="CalMD"/>
-            <output name="calmd" file="match_chr21_DBA_974.filt1.dedup.bamleft.calmd.bam" ftype="bam" />
-        </test>
     </tests>
     <help>
 ARTbio bam cleaning overview