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changeset 1:e23c455f8335 draft

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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
author bgruening
date Wed, 01 Mar 2017 17:15:13 -0500
parents 8abf4c0a4af0
children 580ee1e91801
files rnaz.xml test-data/rnaz_input_trna.aln test-data/rnaz_result2.fasta test-data/rnaz_result_trna.fasta test-data/rnaz_test_input2.aln tool_dependencies.xml
diffstat 6 files changed, 201 insertions(+), 45 deletions(-) [+]
line wrap: on
line diff
--- a/rnaz.xml	Sat Feb 22 08:00:02 2014 -0500
+++ b/rnaz.xml	Wed Mar 01 17:15:13 2017 -0500
@@ -1,49 +1,154 @@
-<tool id="rnaz" name="RNAz" version="0.1.0">
+<tool id="rnaz" name="RNAz" version="2.1.0">
     <description>predicting structurally conserved and thermodynamically stable RNA secondary structures</description>
-    <version_command>RNAz --version</version_command>
+
     <requirements>
         <requirement type="package" version="2.1">rnaz</requirement>
     </requirements>
-    <command>
-RNAz $input
-$forward
-$reverse
-$both_strands
-$dinucleotide
-$mononucleotide
-$locarnate
-$no_shuffle
-#if $cutoff_p != -1:
---cutoff=$cutoff_p
-#end if
-&gt; temp.txt;
-grep -v -E "^ |^#|^$" temp.txt &gt; $outfile;
-grep -E "^ |^#|^$" temp.txt;
-    </command>
+
     <stdio>
         <exit_code range="1:" level="fatal" description="Error occurred. Please check Tool Standard Error" />
         <exit_code range=":-1" level="fatal" description="Error occurred. Please check Tool Standard Error" />
     </stdio>
+
+    <version_command>RNAz --version</version_command>
+    <command>
+<![CDATA[
+    RNAz '$input'
+        --$forward_or_reverse
+        $dinucleotide
+        $mononucleotide
+        $locarnate
+        $noshuffle
+        #if $cutoff != -1.0:
+        --cutoff=$cutoff
+        #end if
+        > temp.txt &&
+        grep -v -E "^ |^#|^$" temp.txt > '$outfile' &&
+        grep -E "^ |^#|^$" temp.txt 
+]]>
+    </command>
     <inputs>
         <param format="txt" name="input" type="data" label="Input Alignment File" />
-        <param name="forward" type="boolean" checked="false" truevalue="--forward" falsevalue="" label="Score forward strand" help="--forward" />
-        <param name="reverse" type="boolean" checked="false" truevalue="--reverse" falsevalue="" label="Score reverse strand" help="--reverse" />
-        <param name="both_strands" type="boolean" checked="false" truevalue="-e" falsevalue="" label="Score both strands" help="-e" />
-        <param name="cutoff_p" label="Probability cutoff" type="float" value="-1" help="-1 to deactivate"/>
-        <param name="dinucleotide" type="boolean" checked="true" truevalue="--dinucleotide" falsevalue="" label="Use dinucleotide shuffled z-scores" help="--dinucleotide" />
-        <param name="mononucleotide" type="boolean" checked="true" truevalue="--mononucleotide" falsevalue="" label="Use mononucleotide shuffled z-scores" help="--mononucleotide" />
-        <param name="locarnate" type="boolean" checked="false" truevalue="--locarnate" falsevalue="" label="Use decision model for structural alignments" help="--locarnate" />
-        <param name="no_shuffle" type="boolean" checked="false" truevalue="--no-shuffle" falsevalue="" label="Never fall back to shuffling" help="--no-shuffle" />
+        <param name="forward_or_reverse" type="select" label="Scored strand">
+            <option value="forward">Score forward strand (-f)</option>
+            <option value="reverse">Score reverse strand (-r)</option>
+            <option value="both-strands">Score both strands (-b)</option>
+        </param>
+        <param argument="--cutoff" label="Probability cutoff" type="float" value="-1.0" help="-1.0 to deactivate"/>
+        <param argument="--dinucleotide" type="boolean" checked="true" truevalue="--dinucleotide" falsevalue="" label="Use dinucleotide shuffled z-scores" />
+        <param argument="--mononucleotide" type="boolean" checked="true" truevalue="--mononucleotide" falsevalue="" label="Use mononucleotide shuffled z-scores"  />
+        <param argument="--locarnate" type="boolean" checked="false" truevalue="--locarnate" falsevalue="" label="Use decision model for structural alignments" />
+        <param argument="--no-shuffle" name="noshuffle"  type="boolean" checked="false" truevalue="--no-shuffle" falsevalue="" label="Never fall back to shuffling"  />
     </inputs>
     <outputs>
         <data name="outfile" format="fasta" />
     </outputs>
-    <help>**What it does**
-     RNAz is a program for predicting structurally conserved and thermodynamically stable RNA secondary structures in multiple sequence alignments. It can be used in genome wide screens to detect functional RNA structures, as found in noncoding RNAs and cis-acting regulatory elements of mRNAs.
+    <tests>
+        <test>
+            <param name="input" value="rnaz_input_trna.aln"/>
+            <output name="outfile" file="rnaz_result_trna.fasta"/> 
+        </test>
+        <test>
+            <param name="input" value="rnaz_test_input2.aln"/>
+            <output name="outfile" file="rnaz_result2.fasta"/> 
+        </test>
+    </tests>
+    <help>
+<![CDATA[
+
+**What it does**
+
+RNAz is a program for predicting structurally conserved and thermodynamically stable RNA secondary structures in multiple sequence alignments. It can be used in genome wide screens to detect functional RNA structures, as found in noncoding RNAs and cis-acting regulatory elements of mRNAs.
+
+**Input**
+
+Input is a multiple sequence alignment file. Currently the the following alignment formats can be read: CLUSTALW, FASTA, PHYLIP,NEXUS, MAF, and XMFA. Alignments can be generated by any sequence based alignment program. Sequence alignment tools can be found in Galaxy too (e.g. ClustalW).
+
+Example: 
+
+CLUSTAL 2.1 multiple sequence alignment
+
+
+sacCer1         GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC
+
+sacKlu          GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGCTAGGGGTTCGAGC
+
+sacBay          GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC
+
+sacCas          GCTTCAGTAGCTCAGTCGGAAGAGCGTCAGTCTCATAATCTGAAGGTCGAGAGTTCGAAC
+
+                \** *   * \** \** \**\** \** \**\**  * *\** \**\**\*  *\**\*    * \**\**\** *
 
 
-Gruber AR, Findeiss, Washietl S, Hofacker IL, and Stadler PF. 
-RNAz 2.0: Improved noncoding rna detection. 
-Pac Symp Biocomput, 2010. 15:69–79.
+**Output**
+
+In Galaxy RNAz gives you 2 output files: a summary file and a result file. For the example input they look like this:
+
+Summary:
+
+ Sequences: 4
+ Columns: 60
+ Reading direction: forward
+ Mean pairwise identity:  82.50
+ Shannon entropy: 0.28395
+ G+C content: 0.51667
+ Mean single sequence MFE: -16.67
+ Consensus MFE: -15.59
+ Energy contribution: -15.53
+ Covariance contribution:  -0.06
+ Combinations/Pair:   1.26
+ Mean z-score:  -0.66
+ Structure conservation index:   0.93
+ Background model: mononucleotide
+ Decision model: sequence based alignment quality
+ SVM decision value:  -0.64
+ SVM RNA-class probability: 0.238023
+ Prediction: OTHER
+ 
+ 
+Result file:
+
+>sacCer1
+
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC
+
+..((((...((((........))))....(((((((((((....))))))))))))))). ( -19.00, z-score =  -1.44, R)
+
+>sacKlu
+
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGCUAGGGGUUCGAGC
+
+..((((...((((........))))....((((((((..(....)..)))))))))))). ( -16.00, z-score =  -0.11, R)
+
+>sacBay
+
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC
+
+..((((...((((........))))....(((((((((((....))))))))))))))). ( -19.00, z-score =  -1.44, R)
+
+>sacCas
+
+GCUUCAGUAGCUCAGUCGGAAGAGCGUCAGUCUCAUAAUCUGAAGGUCGAGAGUUCGAAC
+
+.((((((..((((........))))..............)))))).(((......))).. ( -12.69, z-score =   0.35, R)
+
+>consensus
+
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC
+
+..((((...((((........))))....(((((((((((....))))))))))))))). (-15.59 = -15.53 +  -0.06) 
+
+
+
+
+
+
+]]>
+
     </help>
+
+    <citations>
+        <citation type="doi">10.1142/9789814295291_0009</citation>
+    </citations>    
+    
 </tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnaz_input_trna.aln	Wed Mar 01 17:15:13 2017 -0500
@@ -0,0 +1,14 @@
+CLUSTAL W (1.83) multiple sequence alignment
+
+
+sacCer1         GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC
+sacBay          GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC
+sacKlu          GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGCTAGGGGTTCGAGC
+sacCas          GCTTCAGTAGCTCAGTCGGAAGAGCGTCAGTCTCATAATCTGAAGGTCGAGAGTTCGAAC
+                ** *   * ** ** **** ** ****  * *** *****  ****    * ****** *
+
+sacCer1         CCCCTACAGGGCT
+sacBay          CCCCTACAGGGCT
+sacKlu          CCCCTACAGGGCT
+sacCas          CTCCCCTGGAGCA
+                * **    * ** 
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnaz_result2.fasta	Wed Mar 01 17:15:13 2017 -0500
@@ -0,0 +1,24 @@
+>AP000397.1_114390-114319
+UGGAGUAUAGCCAAG--UGG--UAAGGCAU-CGGUUUUUGGUACCGGCAU---------GCAAAGGUUCGAAUCCUUUUACUCCAG
+(((((((..(((...--.))--)...((((-((((.......))))..))---------))(((((.......)))))))))))). ( -22.00, z-score =  -1.73, R)
+>X03715.1_388-461
+CGGAAAGUAGCUUAGCUUGG--UAGAGCAC-UCGGUUUGGGACCGAGGGG---------UCGCAGGUUCGAAUCCUGUCUUUCCGA
+(((((((..((((.((((..--..)))).(-((((((...))))))))))---------).(((((.......)))))))))))). ( -28.90, z-score =  -2.35, R)
+>U67517.1_7511-7582
+GCCGGGGUGGGGUAG--UGG--CCAUCCUGGGGGACUGUGGAUCCC-CUG---------ACCCGGGUUCAAUUCCCGGUCCCGGCC
+.(((((((((.....--...--)))))))))(((((((.(((..((-(..---------....)))......)))))))))).... ( -35.60, z-score =  -1.36, R)
+>X99256.1_11558-11626
+GUAAACAUAGUUUA----AU--CAAAACAU-UAGAUUGUGAAUCUAACA----------AUAGAGGCUCGAAACCUCUUGCUUACC
+((((.(((((((((----((--......))-))))))))).........----------..(((((.......)))))...)))). ( -13.90, z-score =  -2.44, R)
+>M10217.1_5910-5978
+AGUAAAGUCAGCUA----AA--AAAGCUUUUGGGCCCAU--ACCCCAAAC---------AUGUUGGUUAAACCCCUUCCUUUACUA
+(((((((..((((.----..--..))))((((((.....--..)))))).---------...................))))))). ( -13.90, z-score =  -2.40, R)
+>AC021639.5_181586-181505
+GCAGUCGUGGCCGAGU---GGUUAAGGCGU-CUGACUCGAAAUCAGAUUCCCUCUGGGAGCGUAGGUUCGAAUCCUACCGGCUGCG
+((((((((((((....---)))))..((((-((((.......))))))((((...))))))(((((.......)))))))))))). ( -31.20, z-score =  -1.31, R)
+>AP000063.1_59179-59095
+GCGGGGGUGCCCGAGCCUGGCCAAAGGGGU-CGGGCUCAGGACCCGAUGGCGUAGGCCUGCGUGGGUUCAAAUCCCACCCCCCGCA
+(((((((((...((((((((((.....)))-))))))).(((((((..(((....)))....)))))))......)).))))))). ( -52.80, z-score =  -4.23, R)
+>consensus
+GCGAAAGUAGCCUAG__UGG__UAAGGCAU_CGGACUCUGGACCCGACAG_________ACGUAGGUUCGAAUCCUACCCCUCCCA
+(((((((..(((.............)))...((((.......))))...............(((((.......)))))))))))). (-27.32 = -19.11 +  -8.22) 
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnaz_result_trna.fasta	Wed Mar 01 17:15:13 2017 -0500
@@ -0,0 +1,15 @@
+>sacCer1
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGCCCCCUACAGGGCU
+(((((((.(((((........))))...((((.((((....))))))))(((((....)))))).))))))). ( -29.20, z-score =  -2.74, R)
+>sacBay
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGCCCCCUACAGGGCU
+(((((((.(((((........))))...((((.((((....))))))))(((((....)))))).))))))). ( -29.20, z-score =  -2.74, R)
+>sacKlu
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGCUAGGGGUUCGAGCCCCCUACAGGGCU
+(((((((.(((((........)))).(((((.......)))))......(((((....)))))).))))))). ( -27.20, z-score =  -1.87, R)
+>sacCas
+GCUUCAGUAGCUCAGUCGGAAGAGCGUCAGUCUCAUAAUCUGAAGGUCGAGAGUUCGAACCUCCCCUGGAGCA
+(((((((..((((........)))).((((.........))))((((((......)).))))...))))))). ( -23.20, z-score =  -1.36, R)
+>consensus
+GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGCCCCCUACAGGGCU
+(((((((..((((........)))).(((((.......))))).....(((((.......)))))))))))). (-26.50 = -23.63 +  -2.87) 
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rnaz_test_input2.aln	Wed Mar 01 17:15:13 2017 -0500
@@ -0,0 +1,12 @@
+CLUSTAL W --- LocARNA 1.9.2pre2
+
+
+
+AP000397.1_114390-114319 UGGAGUAUAGCCAAG--UGG--UAAGGCAU-CGGUUUUUGGUACCGGCAU---------GCAAAGGUUCGAAUCCUUUUACUCCAG
+X03715.1_388-461         CGGAAAGUAGCUUAGCUUGG--UAGAGCAC-UCGGUUUGGGACCGAGGGG---------UCGCAGGUUCGAAUCCUGUCUUUCCGA
+U67517.1_7511-7582       GCCGGGGUGGGGUAG--UGG--CCAUCCUGGGGGACUGUGGAUCCC-CUG---------ACCCGGGUUCAAUUCCCGGUCCCGGCC
+X99256.1_11558-11626     GUAAACAUAGUUUA----AU--CAAAACAU-UAGAUUGUGAAUCUAACA----------AUAGAGGCUCGAAACCUCUUGCUUACC
+M10217.1_5910-5978       AGUAAAGUCAGCUA----AA--AAAGCUUUUGGGCCCAU--ACCCCAAAC---------AUGUUGGUUAAACCCCUUCCUUUACUA
+AC021639.5_181586-181505 GCAGUCGUGGCCGAGU---GGUUAAGGCGU-CUGACUCGAAAUCAGAUUCCCUCUGGGAGCGUAGGUUCGAAUCCUACCGGCUGCG
+AP000063.1_59179-59095   GCGGGGGUGCCCGAGCCUGGCCAAAGGGGU-CGGGCUCAGGACCCGAUGGCGUAGGCCUGCGUGGGUUCAAAUCCCACCCCCCGCA
+
--- a/tool_dependencies.xml	Sat Feb 22 08:00:02 2014 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,14 +0,0 @@
-<tool_dependency>
-    <package name="rnaz" version="2.1">
-        <install version="1.0">
-            <actions>
-                <action type="download_by_url">http://www.tbi.univie.ac.at/~wash/RNAz/RNAz-2.1.tar.gz</action>
-                <action type="autoconf" />
-                <action type="set_environment">
-                    <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable>
-                </action>
-            </actions>
-        </install>
-        <readme>Compiling RNAz requires a C compiler (typically gcc)</readme>
-    </package>
-</tool_dependency>