Mercurial > repos > devteam > bowtie_color_wrappers
changeset 2:393c6829d3c1 draft
Convert tool to use $GALAXY_SLOTS if available.
author | Nate Coraor <nate@bx.psu.edu> |
---|---|
date | Thu, 16 Jan 2014 12:48:21 -0500 |
parents | 2506bd84cc54 |
children | 065886169d72 |
files | bowtie_color_wrapper.xml |
diffstat | 1 files changed, 6 insertions(+), 6 deletions(-) [+] |
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--- a/bowtie_color_wrapper.xml Mon Apr 01 14:30:05 2013 -0400 +++ b/bowtie_color_wrapper.xml Thu Jan 16 12:48:21 2014 -0500 @@ -5,8 +5,8 @@ <description></description> <command interpreter="python"> bowtie_wrapper.py - ## Hackish setting of number of threads - --threads="4" + ## Set number of threads + --threads="\${GALAXY_SLOTS:-4}" ## Outputs --output="${output}" #if str( $singlePaired.sPaired ) == "single" @@ -402,7 +402,7 @@ Bowtie command: bowtie -q -p 4 -S +sam-nohead -C chrM_color test-data/bowtie_in1.fastqcssanger > bowtie_out1_u.sam sort bowtie_out1_u.sam > bowtie_out1.sam - -p is the number of threads, which is hardcoded above. You need to replace the + with 2 dashes. + -p is the number of threads. You need to replace the + with 2 dashes. chrM_color needs to be the base location/name of the index files. --> <param name="genomeSource" value="indexed" /> @@ -423,7 +423,7 @@ sort bowtie_out3_u_2.sam > bowtie_out3_2.sam Then also need to modify bowtie_out3_1.sam and bowtie_out3_2.sam so that all @ lines come before sequence lines. The two unmapped output files will be named bowtie_out4_1.fastq and bowtie_out4_2.fastq - -p is the number of threads, hardcoded above. You need to replace the + with 2 dashes. + -p is the number of threads. You need to replace the + with 2 dashes. chrM_base is the index files' location/base name. --> <param name="genomeSource" value="history" /> @@ -471,7 +471,7 @@ Bowtie command: bowtie -q -p 4 -S +sam-nohead -C -n 2 -e 70 -l 28 +maxbts 125 -k 1 +snpfrac 0.001 +col-keepends chrM_color test-data/bowtie_in1.fastqcssanger > bowtie_out4_u.sam sort bowtie_out4_u.sam > bowtie_out4.sam - -p is the number of threads, hardcoded above. You need to replace the + with 2 dashes. + -p is the number of threads. You need to replace the + with 2 dashes. chrM_base is the index files' location/base name. --> <param name="genomeSource" value="indexed" /> @@ -510,7 +510,7 @@ bowtie-build +noauto +bmaxdivn 4 +dcv 1024 +offrate 5 +ftabchars 10 +little -C -f test-data/chr_m.fasta chrM_color bowtie -q -X 1000 +ff -p 4 -S +sam-nohead -C chrM_color -1 test-data/bowtie_in3.fastqcssanger -2 test-data/bowtie_in4.fastqcssanger > bowtie_out5_u.sam sort bowtie_out5_u.sam > bowtie_out5.sam - -p is the number of threads, hardcoded above. You need to replace the + with 2 dashes. + -p is the number of threads. You need to replace the + with 2 dashes. chrM_base is the index files' location/base name. --> <param name="genomeSource" value="history" />