Mercurial > repos > ebi-gxa > salmon_kallisto_mtx_to_10x

<tool id="_salmon_kallisto_mtx_to_10x" name="salmonKallistoMtxTo10x" version="0.0.1+galaxy5" profile="18.01">
    <description>Transforms .mtx matrix and associated labels into a format compatible with tools expecting old-style 10X data</description>
    <requirements>
      <requirement type="package" version="1.4.1">scipy</requirement>
      <requirement type="package" version="1.0.1">pandas</requirement>
      <requirement type="package" version="5.37">file</requirement>
    </requirements>
    <command detect_errors="exit_code"><![CDATA[
        file $mtx_file | grep 'gzip compressed' > /dev/null;
        if [ $? -eq 0 ]; then
            matrixfile=matrix.mtx.gz;
        else
            matrixfile=matrix.mtx;
        fi;
        ln -s ${mtx_file} \$matrixfile;
        $__tool_directory__/salmonKallistoMtxTo10x.py --cell_prefix "${cell_prefix}" "\${matrixfile}" "${genes_file}" "${barcodes_file}" ./
	    ]]></command>

    <inputs>
        <param name="mtx_file" type="data" format="mtx" label=".mtx-format matrix" />
        <param name="genes_file" type="data" format="txt" label="Tab-delimited genes file" />
        <param name="barcodes_file" type="data" format="txt" label="Tab-delimited barcodes file" />
        <param name="cell_prefix" type="text" optional='true' value="" label="Prefix to prepend to cell names / barcodes" help="This is useful when multiple matrices from different libraries with overlapping barcodes will be merged"/>
    </inputs>

    <outputs>
        <data name="genes_out" format="tabular" from_work_dir="genes.tsv" label="${tool.name} on ${on_string}: genes"/>
        <data name="barcodes_out" format="tabular" from_work_dir="barcodes.tsv" label="${tool.name} on ${on_string}: barcodes"/>
        <data name="matrix_out" format="mtx" from_work_dir="matrix.mtx" label="${tool.name} on ${on_string}: matrix" />
    </outputs>

    <help><![CDATA[
.. class:: infomark

**What it does**

Kallisto and Alevin (and possibly other tools) output an MTX file and associated labels that are not consistent with the old-style 10X, meaning that routines designed to parse those files cannot be used. This tool transforms (in the mathematical sense) the matrix, and reformats the genes file (essentially duplicating the column) to match those earlier conventions.

**Inputs**

MTX and row and gene labels from the relevant tool. For Alevin this will be

 * Matrix file: quants_mat.mtx.gz
 * Genes file: quants_mat_cols.txt
 * Barcodes file: quants_mat_rows.txt

For Kallisto it will be:

 * Matrix file: [name].mtx.gz
 * Genes file: [name].genes.txt
 * Barcodes file: [name].barcodes.txt

-----

**Outputs**

 * MTX, gene and cell labels in 10X style
]]></help>
<citations>
  <citation type="bibtex">
@misc{github-hinxton-single-cell,
author = {Jonathan Manning, EBI Gene Expression Team},
year = {2019},
title = {Hinxton Single Cell Anlysis Environment},
publisher = {GitHub},
journal = {GitHub repository},
url = {https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary},
  }</citation>
</citations>
</tool>
author	ebi-gxa
date	Wed, 11 Mar 2020 11:28:41 -0400
parents	5675dc87bb8d
children	88f5a0a6a818