changeset 2:c23d0ff783d4 draft

planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit ccd9b839849600b1aa3b6cf54f667575a5f2da9d
author ebi-gxa
date Fri, 25 Oct 2019 08:25:27 -0400
parents dcfb23758646
children 8e48fa9618c1
files dataset_102795.dat dataset_102795.dat.zip input.h5 input.h5.zip scanpy-filter-cells.xml scanpy_macros2.xml
diffstat 6 files changed, 32534 insertions(+), 12 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/dataset_102795.dat	Fri Oct 25 08:25:27 2019 -0400
@@ -0,0 +1,32520 @@
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Binary file dataset_102795.dat.zip has changed
Binary file input.h5 has changed
Binary file input.h5.zip has changed
--- a/scanpy-filter-cells.xml	Mon Sep 16 08:19:52 2019 -0400
+++ b/scanpy-filter-cells.xml	Fri Oct 25 08:25:27 2019 -0400
@@ -12,15 +12,15 @@
     --gene-name '${gene_name}'
 #end if
 #if $parameters
-    #set pars = ' '.join(['--param {name} {min} {max}'.format(**$p) for $p in $parameters])
+    #set pars = ' '.join(['--param c:{name} {min} {max}'.format(**$p) for $p in $parameters])
     ${pars}
 #end if
 #if $categories
-    #set cats = ' '.join(['--category {name} {values}'.format(**$c) for $c in $categories])
+    #set cats = ' '.join(['--category c:{name} {values}'.format(**$c) for $c in $categories])
     ${cats}
 #end if
 #if $subsets
-    #set subs = ' '.join(['--subsets {name} {subset}'.format(**$s) for $c in $subsets])
+    #set subs = ' '.join(['--subset c:{name} {subset}'.format(**$s) for $s in $subsets])
     ${subs}
 #end if
     @INPUT_OPTS@
@@ -31,14 +31,14 @@
     <expand macro="input_object_params"/>
     <expand macro="output_object_params"/>
 
-    <param name="gene_name" type="text" value="index" label="Name of the column in `anndata.var` that contains gene name"
-           help="This is used for flagging mitochondria genes (starting with 'MT-')"/>
+    <param name="gene_name" type="text" optional="true" label="Name of the column in `anndata.var` that contains gene name"
+           help="Used for flagging mitochondria genes (starting with 'MT-'). Leave empty if gene table already has a boolean column called 'mito' that flags MT genes"/>
 
     <repeat name="parameters" title="Parameters used to filter cells" min="1">
       <param name="name" type="text" value="n_genes" label="Name of parameter to filter on" help="for example n_genes or n_counts">
         <option value="n_genes">n_genes</option>
-        <option value="cell:n_counts">n_counts</option>
-        <option value="pct_counts_mito">pct_counts_mito</option>
+        <option value="n_counts">n_counts</option>
+        <option value="pct_counts_mito">pct_counts_mito (only usable if MT genes are flagged)</option>
       </param>
       <param name="min" type="float" value="0" min="0" label="Min value"/>
       <param name="max" type="float" value="1e9" label="Max value"/>
@@ -46,12 +46,12 @@
 
     <repeat name="categories" title="Categories used to filter cells" min="0">
       <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/>
-      <param name="values" type="text" value="" label="Comma-separated values"/>
+      <param name="values" type="text" value="" label="Comma-separated values to keep"/>
     </repeat>
 
     <repeat name="subsets" title="Subsets used to filter cells" min="0">
       <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/>
-      <param name="subset" type="data" format="tabular" label="List of values"/>
+      <param name="subset" type="data" format="tabular" label="List of values to keep" help="A one-column headerless text file is required"/>
     </repeat>
     <expand macro="export_mtx_params"/>
   </inputs>
@@ -72,7 +72,7 @@
         <param name="max" value="2500"/>
       </repeat>
       <repeat name="parameters">
-        <param name="name" value="cell:n_counts"/>
+        <param name="name" value="n_counts"/>
         <param name="min" value="0"/>
         <param name="max" value="1e9"/>
       </repeat>
--- a/scanpy_macros2.xml	Mon Sep 16 08:19:52 2019 -0400
+++ b/scanpy_macros2.xml	Fri Oct 25 08:25:27 2019 -0400
@@ -1,9 +1,11 @@
 <macros>
-  <token name="@TOOL_VERSION@">1.4.2</token>
+  <token name="@TOOL_VERSION@">1.4.3</token>
   <token name="@HELP@">More information can be found at https://scanpy.readthedocs.io</token>
   <token name="@VERSION_HISTORY@"><![CDATA[
 **Version history**
 
+1.4.3+galaxy0: Update to scanpy-scripts 0.2.5 (running scanpy ==1.4.3).
+
 1.4.2+galaxy0: Update to scanpy-scripts 0.2.4 (requires scanpy >=1.4.2).
 
 1.3.2+galaxy1: Normalise-data and filter-genes: Exposes ability to output 10x files.
@@ -31,7 +33,7 @@
 
   <xml name="requirements">
     <requirements>
-      <requirement type="package" version="0.2.4.post4">scanpy-scripts</requirement>
+      <requirement type="package" version="0.2.5.post1">scanpy-scripts</requirement>
       <yield/>
     </requirements>
   </xml>