Mercurial > repos > jjohnson > mothur_toolsuite
annotate mothur/tools/mothur/align.seqs.xml @ 1:fcc0778f6987
Migrated tool version 1.16.0 from old tool shed archive to new tool shed repository
author | jjohnson |
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date | Tue, 07 Jun 2011 17:35:35 -0400 |
parents | 3202a38e44d9 |
children | e990ac8a0f58 |
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1 <tool id="mothur_align_seqs" name="Align.seqs" version="1.16.0"> |
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2 <description>Align sequences to a template alignment</description> |
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3 <command interpreter="python"> |
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4 mothur_wrapper.py |
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5 --cmd='align.seqs' |
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6 --result='^mothur.\S+\.logfile$:'$logfile,'^\S+\.align$:'$out_file,'^\S+\.align\.report$:'$report |
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7 --outputdir='$logfile.extra_files_path' |
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8 --candidate=$candidate |
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9 --template=$alignment.template |
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10 #if $search.method == 'kmer': |
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11 --ksize=$search.ksize |
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12 #else: |
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13 --search=$search.method |
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14 #end if |
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15 --align=$align |
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16 #if $scoring.adjust == 'yes': |
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17 --match=$scoring.match |
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18 --mismatch=$scoring.mismatch |
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19 --gapopen=$scoring.gapopen |
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20 --gapextend=$scoring.gapextend |
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21 #end if |
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22 #if $reverse.flip == 'yes': |
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23 --flip=true |
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24 --threshold=$reverse.threshold |
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25 #end if |
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26 --processors=2 |
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27 </command> |
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28 <inputs> |
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29 <param name="candidate" type="data" format="fasta" label="candidate - Candiate Sequences"/> |
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30 <conditional name="alignment"> |
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31 <param name="source" type="select" label="Select Template from" help=""> |
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32 <option value="hist">History</option> |
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33 <option value="ref">Cached Reference</option> |
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34 </param> |
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35 <when value="ref"> |
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36 <param name="template" type="select" label="template - Select an alignment database " help=""> |
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37 <options from_file="mothur_aligndb.loc"> |
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38 <column name="name" index="0" /> |
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39 <column name="value" index="1" /> |
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40 </options> |
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41 </param> |
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42 </when> |
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43 <when value="hist"> |
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44 <param name="template" type="data" format="fasta" label="template - Template to align with" help=""/> |
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45 </when> |
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46 </conditional> |
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47 <conditional name="search"> |
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48 <param name="method" type="select" label="Select a search method" help=""> |
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49 <option value="kmer">kmer (default)</option> |
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50 <option value="suffix">suffix tree</option> |
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51 <option value="blast">blast</option> |
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52 </param> |
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53 <when value="kmer"> |
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54 <param name="ksize" type="integer" value="8" label="ksize - kmer length between 5 and 12"> |
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55 <validator type="in_range" message="ksize - kmer length between 5 and 12" min="5" max="12"/> |
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56 </param> |
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57 </when> |
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58 <when value="suffix"/> |
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59 <when value="blast"/> |
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60 </conditional> |
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61 <param name="align" type="select" label="align - Select a pairwise alignment method" help=""> |
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62 <option value="needleman">needleman (default)</option> |
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63 <option value="gotoh">gotoh</option> |
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64 <option value="blast">blast</option> |
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65 </param> |
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66 |
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67 <conditional name="scoring"> |
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68 <param name="adjust" type="select" label="Alignment scoring values" help=""> |
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69 <option value="no">use defaults</option> |
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70 <option value="yes">adjust values</option> |
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71 </param> |
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72 <when value="no"/> |
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73 <when value="yes"> |
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74 <param name="match" type="integer" value="1" label="match - Pairwise alignment reward for a match"/> |
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75 <param name="mismatch" type="integer" value="-1" label="mismatch - Pairwise alignment penalty for a mismatch"/> |
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76 <param name="gapopen" type="integer" value="-2" label="gapopen - Pairwise alignment penalty for opening a gap"/> |
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77 <param name="gapextend" type="integer" value="-1" label="gapextend - Pairwise alignment penalty for extending a gap"/> |
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78 </when> |
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79 </conditional> |
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80 |
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81 <conditional name="reverse"> |
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82 <param name="flip" type="select" label="flip - Try to align against the reverse complement" help=""> |
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83 <option value="no">No</option> |
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84 <option value="yes">Yes values</option> |
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85 </param> |
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86 <when value="no"/> |
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87 <when value="yes"> |
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88 <param name="threshold" type="float" value=".5" label="threshold - Cutoff (0. - 1.) at which an alignment is deemed 'bad' and the reverse complement may be tried."/> |
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89 </when> |
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90 </conditional> |
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91 |
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92 </inputs> |
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93 <outputs> |
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94 <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" /> |
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95 <data format="align" name="out_file" label="${tool.name} on ${on_string}: align" /> |
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96 <data format="align.report" name="report" label="${tool.name} on ${on_string}: align.report" /> |
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97 </outputs> |
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98 <requirements> |
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99 <requirement type="binary">mothur</requirement> |
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100 </requirements> |
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101 <tests> |
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102 </tests> |
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103 <help> |
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104 **Mothur Overview** |
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105 |
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106 Mothur_, initiated by Dr. Patrick Schloss and his software development team |
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107 in the Department of Microbiology and Immunology at The University of Michigan, |
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108 provides bioinformatics for the microbial ecology community. |
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109 |
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110 .. _Mothur: http://www.mothur.org/wiki/Main_Page |
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111 |
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112 **Command Documenation** |
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113 |
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114 The align.seqs_ command aligns a user-supplied fasta-formatted candidate sequence file to a user-supplied fasta-formatted template alignment. |
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115 |
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116 .. _align.seqs: http://www.mothur.org/wiki/Align.seqs |
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117 |
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118 |
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119 </help> |
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120 </tool> |