Mercurial > repos > peterjc > tmhmm_and_signalp
annotate tools/protein_analysis/wolf_psort.xml @ 16:7de64c8b258d draft
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author | peterjc |
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date | Wed, 18 Sep 2013 06:16:58 -0400 |
parents | 6365217cd3de |
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1 <tool id="wolf_psort" name="WoLF PSORT" version="0.0.6"> |
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2 <description>Eukaryote protein subcellular localization prediction</description> |
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3 <command interpreter="python"> |
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4 wolf_psort.py $organism "\$NSLOTS" "$fasta_file" "$tabular_file" |
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5 ##I want the number of threads to be a Galaxy config option... |
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6 ##Set the number of threads in the runner entry in universe_wsgi.ini |
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7 ##which (on SGE at least) will set the $NSLOTS environment variable. |
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8 ##If the environment variable isn't set, get "", and python wrapper |
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9 ##defaults to four threads. |
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10 </command> |
9 | 11 <stdio> |
12 <!-- Anything other than zero is an error --> | |
13 <exit_code range="1:" /> | |
14 <exit_code range=":-1" /> | |
15 </stdio> | |
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16 <inputs> |
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17 <param name="fasta_file" type="data" format="fasta" label="FASTA file of protein sequences"/> |
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18 <param name="organism" type="select" display="radio" label="Organism"> |
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19 <option value="animal">Animal</option> |
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20 <option value="plant">Plant</option> |
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21 <option value="fungi">Fungi</option> |
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22 </param> |
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23 </inputs> |
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24 <outputs> |
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25 <data name="tabular_file" format="tabular" label="WoLF PSORT $organism results" /> |
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26 </outputs> |
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27 <requirements> |
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28 <requirement type="binary">runWolfPsortSummary</requirement> |
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29 </requirements> |
14 | 30 <tests> |
31 <test> | |
32 <param name="fasta_file" value="four_human_proteins.fasta" ftype="fasta"/> | |
33 <param name="organism" value="animal"/> | |
34 <output name="tabular_file" file="four_human_proteins.wolf_psort.tabular" ftype="tabular"/> | |
35 </test> | |
36 <test> | |
37 <param name="fasta_file" value="empty.fasta" ftype="fasta"/> | |
38 <param name="organism" value="animal"/> | |
39 <output name="tabular_file" file="empty_wolf_psort.tabular" ftype="tabular"/> | |
40 </test> | |
41 <test> | |
42 <param name="fasta_file" value="empty.fasta" ftype="fasta"/> | |
43 <param name="organism" value="plant"/> | |
44 <output name="tabular_file" file="empty_wolf_psort.tabular" ftype="tabular"/> | |
45 </test> | |
46 <test> | |
47 <param name="fasta_file" value="empty.fasta" ftype="fasta"/> | |
48 <param name="organism" value="fungi"/> | |
49 <output name="tabular_file" file="empty_wolf_psort.tabular" ftype="tabular"/> | |
50 </test> | |
51 </tests> | |
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52 <help> |
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53 |
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54 **What it does** |
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55 |
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56 This calls the WoLF PSORT tool for prediction of eukaryote protein subcellular localization. |
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57 |
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58 The input is a FASTA file of protein sequences, and the output is tabular with four columns (multiple rows per protein): |
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59 |
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60 ====== =================== |
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61 Column Description |
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62 ------ ------------------- |
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63 1 Sequence identifier |
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64 2 Compartment |
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65 3 Score |
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66 4 Prediction rank |
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67 ====== =================== |
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68 |
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69 |
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70 **Localization Compartments** |
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71 |
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72 The table below gives the WoLF PSORT localization site definitions, and the corresponding Gene Ontology (GO) term. |
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73 |
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74 ====== ===================== ===================== |
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75 Abbrev Localization Site GO Cellular Component |
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76 ------ --------------------- --------------------- |
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77 chlo chloroplast 0009507, 0009543 |
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78 cyto cytosol 0005829 |
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79 cysk cytoskeleton 0005856(2) |
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80 E.R. endoplasmic reticulum 0005783 |
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81 extr extracellular 0005576, 0005618 |
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82 golg Golgi apparatus 0005794(1) |
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83 lyso lysosome 0005764 |
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84 mito mitochondria 0005739 |
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85 nucl nuclear 0005634 |
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86 pero peroxisome 0005777(2) |
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87 plas plasma membrane 0005886 |
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88 vacu vacuolar membrane 0005774(2) |
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89 ====== ===================== ===================== |
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90 |
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91 Numbers in parentheses, such as "0005856(2)" indicate that descendant "part_of" |
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92 cellular components were also included, up to the specified depth (2 in this case). |
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93 For example, all of the children and grandchildren of "GO:0005856" were |
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94 included as "cysk". |
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95 |
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96 Additionally compound predictions like mito_nucl are also given. |
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97 |
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98 |
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99 **Notes** |
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100 |
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101 The raw output from WoLF PSORT looks like this (space separated), showing two proteins: |
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102 |
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103 ================================ ============================================ |
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104 gi|301087619|ref|XP_002894699.1| extr 12, mito 4, E.R. 3, golg 3, mito_nucl 3 |
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105 gi|301087623|ref|XP_002894700.1| extr 21, mito 2, cyto 2, cyto_mito 2 |
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106 ================================ ============================================ |
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107 |
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108 This is reformatted into a tabular file as follows for use in Galaxy: |
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109 |
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110 ================================ =========== ===== ==== |
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111 #ID Compartment Score Rank |
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112 -------------------------------- ----------- ----- ---- |
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113 gi|301087619|ref|XP_002894699.1| extr 12 1 |
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114 gi|301087619|ref|XP_002894699.1| mito 4 2 |
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115 gi|301087619|ref|XP_002894699.1| E.R. 3 3 |
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116 gi|301087619|ref|XP_002894699.1| golg 3 4 |
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117 gi|301087619|ref|XP_002894699.1| mito_nucl 3 5 |
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118 gi|301087623|ref|XP_002894700.1| extr 21 1 |
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119 gi|301087623|ref|XP_002894700.1| mito 2 2 |
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120 gi|301087623|ref|XP_002894700.1| cyto 2 3 |
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121 gi|301087623|ref|XP_002894700.1| cyto_mito 2 4 |
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122 ================================ =========== ===== ==== |
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123 |
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124 This way you can easily filter for things like having a top prediction for |
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125 mitochondria (c2=='mito' and c4==1), or extracellular with a score of at |
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126 least 10 (c2=='extr' and 10<=c3), and so on. |
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127 |
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128 |
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129 **References** |
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130 |
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131 If you use this Galaxy tool in work leading to a scientific publication please |
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132 cite the following papers: |
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133 |
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134 Peter J.A. Cock, Björn A. Grüning, Konrad Paszkiewicz and Leighton Pritchard (2013). |
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135 Galaxy tools and workflows for sequence analysis with applications |
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136 in molecular plant pathology. PeerJ 1:e167 |
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137 http://dx.doi.org/10.7717/peerj.167 |
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138 |
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139 Paul Horton, Keun-Joon Park, Takeshi Obayashi, Naoya Fujita, Hajime Harada, C.J. Adams-Collier, and Kenta Nakai (2007). |
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140 WoLF PSORT: Protein Localization Predictor. |
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141 Nucleic Acids Research, 35(S2), W585-W587. |
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142 http://dx.doi.org/10.1093/nar/gkm259 |
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143 |
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144 Paul Horton, Keun-Joon Park, Takeshi Obayashi and Kenta Nakai (2006). |
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145 Protein Subcellular Localization Prediction with WoLF PSORT. |
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146 Proceedings of the 4th Annual Asia Pacific Bioinformatics Conference APBC06, Taipei, Taiwan. pp. 39-48. |
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147 |
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148 See also http://wolfpsort.org |
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149 |
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150 This wrapper is available to install into other Galaxy Instances via the Galaxy |
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151 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp |
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152 </help> |
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153 </tool> |