annotate omero_roi_import.xml @ 1:e957793051a8 draft

planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit a86e6a3189535d12ade221ffbeeaa9d04ad39583
author ufz
date Fri, 06 Sep 2024 08:26:12 +0000
parents 9051b91545f6
children 4f4dc352d660
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1 <tool id="omero_roi_import" name="OMERO ROI Import" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"
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2 profile="20.01" license="MIT">
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3 <description> with ezomero </description>
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4 <macros>
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5 <token name="@TOOL_VERSION@">5.18.0</token>
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6 <token name="@VERSION_SUFFIX@">2</token>
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7 </macros>
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8 <xrefs>
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9 <xref type="bio.tools">omero</xref>
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10 </xrefs>
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11 <requirements>
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12 <requirement type="package" version="3.0.1">ezomero</requirement>
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13 <requirement type="package" version="2.2.2">pandas</requirement>
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14 <!-- openjdk is needed: https://github.com/conda-forge/omero-py-feedstock/pull/16 -->
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15 <requirement type="package" version="21.0.2">openjdk</requirement>
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16 </requirements>
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17 <command detect_errors="exit_code"><![CDATA[
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18 python '$__tool_directory__/omero_roi_upload.py'
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19 --input_file '$input'
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20 --image_id $id
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21 --user '$__user__.extra_preferences.get("omero_account|username", $test_username)'
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22 --psw '$__user__.extra_preferences.get("omero_account|password", $test_password)'
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23 --host '$omero_host'
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24 --port $omero_port
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25 --log_file '$log'
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26 ]]></command>
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27 <inputs>
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28 <param argument="input" type="data" format="tabular" optional="false" label="Tab File with ROIs" help="Select ROIs Tabular file"/>
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29 <param argument="id" type="integer" value="" optional="false" label="Image ID where annotate the ROIs"/>
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30 <param name="omero_host" type="text" label="OMERO host URL">
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31 <validator type="regex" message="Enter a valid host location, for example, your.omero.server">^[a-zA-Z0-9._-]*$</validator>
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32 <validator type="expression" message="No two dots (..) allowed">'..' not in value</validator>
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33 </param>
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34 <param argument="omero_port" type="integer" optional="false" value="4064" label="OMERO port"/>
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35 <param name="test_username" type="hidden" value=""/>
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36 <param name="test_password" type="hidden" value=""/>
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37 </inputs>
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38 <outputs>
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39 <data name="log" format="txt"/>
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40 </outputs>
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41 <tests>
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42 <test>
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43 <param name="omero_host" value="host.docker.internal"/>
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44 <param name="omero_port" value="6064"/>
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45 <param name="id" value="1"/>
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46 <param name="input" value="input_roi.tsv"/>
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47 <param name="test_username" value="root"/>
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48 <param name="test_password" value="omero"/>
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49 <output name="log" value="output_table_roi.txt" ftype="txt">
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50 <assert_contents>
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51 <has_text text="ROI ID: 7 for row 7"/>
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52 </assert_contents>
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53 </output>
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54 </test>
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55 </tests>
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56 <help>
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57
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58 Description
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59 -----------
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60
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61 Tool to upload Regions of Interest (ROIs) to an OMERO server based on shape data provided
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62 in a tabular format (TSV file). The tool reads the shape information from the TSV file, creates the
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63 corresponding ROIs in OMERO, and links them to a specified image.
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64
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65 **Column Headers**:
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66
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67 - shape, x, y, x_rad, y_rad, width, height, label, fontSize, x1, y1, x2, y2, points, fill_color, stroke_color, stroke_width, z, c, t, roi_name, roi_description
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68
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69 - *Shape Type*:
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70
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71 The **shape** column indicates the type of shape being defined, such as Ellipse, Label, Line, Point, Polygon, Polyline, or Rectangle.
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72
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73 - *Position and Dimensions*:
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74
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75 The columns **x**, **y**, **x_rad**, **y_rad**, **width**, and **height** specify the position and dimensions of the shapes, where applicable. For example, Ellipse uses x, y, x_rad, and y_rad, while Rectangle uses x, y, width, and height.
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76
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77 - *Text Labels*:
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78
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79 The **label** and **fontSize** columns are used for the Label shape, specifying the text content and font size.
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80
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81 - *Line Coordinates*:
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82
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83 The columns **x1**, **y1**, **x2**, and **y2** are used for defining the start and end points of a Line.
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84
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85 - *Point Coordinates*:
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86
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87 The **points** column is used for defining multiple points in shapes like Polygon and Polyline. The points are listed as coordinate pairs.
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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88
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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89 - *Colors*:
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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90
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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91 The **fill_color** and **stroke_color** columns define the fill and stroke colors of the shapes in RGBA format.
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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92
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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93 - *Stroke Width*:
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94
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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95 The **stroke_width** column specifies the width of the stroke or border around the shapes.
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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96
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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97 - *Z, C, T Coordinates*:
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98
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99 The **z**, **c**, and **t** columns indicate the Z-plane, channel, and time point to which the shape is associated in the image stack.
9051b91545f6 planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1
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100
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101 - *ROI Identification*:
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102
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103 The **roi_name** and **roi_description** columns provide a name and description for each ROI, allowing for easy identification and documentation within OMERO.
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104
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105 </help>
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106 <citations>
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107 <citation type="doi">10.1038/nmeth.1896</citation>
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108 </citations>
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109 </tool>