Mercurial > repos > tduigou > dnaweaver
changeset 0:90025c4cd50e draft
"planemo upload commit 2f1af427fa4c4f2aad53ab94c4cdb51456c66019-dirty"
author | tduigou |
---|---|
date | Fri, 17 Dec 2021 16:26:13 +0000 |
parents | |
children | c519517e3ade |
files | dnaweaver.xml test-data/assembly_plan_lycopene.xlsx test-data/constructs_lycopene.xml |
diffstat | 3 files changed, 9041 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/dnaweaver.xml Fri Dec 17 16:26:13 2021 +0000 @@ -0,0 +1,130 @@ +<tool id="DNAWeaver" name="DNA Weaver" version="1.0.1"> + <description>Given a SBOL input, calculate assembly parts for Gibson or Golden Gate</description> + <requirements> + <requirement type="package" version="1.0.1">dnaweaver_synbiocad</requirement> + </requirements> + <stdio> + <regex match="WARNING:" level="warning" /> + <regex match="ERROR:" level="fatal" /> + </stdio> + <command detect_errors="exit_code"><![CDATA[ + python -m dnaweaver_synbiocad + '$input' + output.xlsx + '$method' + --constructs '$adv.max_constructs' + && cp output.xlsx '$output' + ]]></command> + <inputs> + <param name="input" type="data" format="xml" label="Constructs" /> + <param name="method" type="select" label="Assembly method"> + <option value="golden_gate" selected="true">Golden Gate</option> + <option value="gibson" >Gibson</option> + <option value="any_method" >Any Method</option> + </param> + <section name="adv" title="Advanced Options" expanded="false"> + <param name="max_constructs" value="-1" type="integer" label="Maximal number of constructs" /> + </section> + </inputs> + <outputs> + <data name="output" format="xlsx" label="Assembly Plan" /> + </outputs> + <tests> + <test> + <!-- test 1: check if identical outputs are produced with default parameters --> + <param name="input" value="constructs_lycopene.xml" /> + <output name="output" file="assembly_plan_lycopene.xlsx" ftype="xlsx" compare="sim_size"/> + </test> + </tests> + <help><![CDATA[ +DNAWeaver +========================== + +This tool uses a multi-objective algorithm to predict the best synthesis and assembling strategy for the previously designed plasmid using either Golden gate assembly, or Gibson assembly, or a mix of both. Given a set of designs (one design is a construct name and list of its parts), it finds a valid and efficient assembly plan to build all the designs. The designs and sequences of parts are provided as an SBOL file (see test.xml for an example). + +Input +----- + +Required: + +* **input**\ : (string) Path to the sbol file(s) +* **method**\ : (string) Assembly method (valid options: golden_gate, gibson, any_method) + +Advanced options: + + +* **--max_constructs**\ : (integer, default=-1) Maximal number of constructs to use. The default -1 corresponds to all. + +Output +------ + + +* **output**\ : (string) Path to the output Excel file + + +Project Links +--------------------- + +* `GitHub <https://edinburgh-genome-foundry.github.io/DnaWeaver>`_ + +Method +-------- + + - We assume that the different standard parts are available or will be ordered, with the exact sequence provided in the input file (in the future it would be easy to automatically break long parts into smaller fragments). + - The desired construct sequence for a genetic part design is simply the concatenation of that design's part sequences in the right order (no assembly overhang is included). + - Buy primers with overhangs to extend the parts fragments via PCR and create homologies between them so they can be assembled together. + - Assemble each construct in a single step with Golden Gate assembly if possible (that is if at least one site out of BsaI, BbsI, and BsmBI is totally absent from the construct sequence), else with Gibson assembly. It can also be only one of the two methods if the option Gibson or golden_gate is selected instead of any_method. + - Start with the first design, and for each subsequence design assembly plan reuse the primers ordered and fragments PCRed in previous designs, if relevant. + +Here is a schema of the supply network used: + +.. image:: https://raw.githubusercontent.com/brsynth/synbiocad-galaxy-wrappers/master/DNAWeaver/img/image4.png + :width: 30 % + :align: center + +| + +The output Excel spreadsheet contains the following sub-sheets: + + - construct_parts: the ID and list of part names (in the right order) for each design. + - construct_sequences: the final sequence of the constructs to build. + - part_sequences: the list of each standard part and its sequence (same information as in the input SBOL file). + - fragment_extensions: for each PCR fragment, the standard part and the primers to use + - assembly_plan: for each design, the list of PCR fragments to use. + - errors: list of errors to help troubleshooting assemblies for which no valid assembly plan was found. + +Limitations +-------------- + + +For constructs with repeated parts and other homologies (such as, in the example, the designs with several "Ter" in a row, ), Gibson assembly (and probably LCR assembly too) may create mis-annealed constructs and more clones will need to be picked. This is not taken into account by the script at the moment. This could be fixed by buying custom fragments from a commercial vendor for extreme cases (i.e. by amending the current implementation to forbid Gibson cuts in regions with homologies elsewhere and add a DNA vendor in the supply network). + +Version +------- + +1.0.1 + +Authors +------- + +* **Valentin Zulkower** +* Melchior du Lac + +License +------- + +`MIT <https://raw.githubusercontent.com/Edinburgh-Genome-Foundry/DnaWeaver-online/master/LICENCE.txt>`_ + +References +--------------- + +`DNA Weaver: optimal DNA assembly strategies via supply networks and shortest-path algorithms <https://www.iwbdaconf.org/2019/docs/IWBDA19Proceedings.pdf>`_ + +Acknowledgments +--------------- + +* Thomas Duigou +* Joan Hérisson + + ]]></help> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/constructs_lycopene.xml Fri Dec 17 16:26:13 2021 +0000 @@ -0,0 +1,8911 @@ +<?xml version="1.0" encoding="utf-8"?> +<rdf:RDF xmlns:dc="http://purl.org/dc/elements/1.1/" + xmlns:dcterms="http://purl.org/dc/terms/" + xmlns:gbconv="http://sbols.org/genBankConversion#" + xmlns:genbank="http://www.ncbi.nlm.nih.gov/genbank#" + xmlns:igem="http://wiki.synbiohub.org/wiki/Terms/igem#" + xmlns:ncbi="http://www.ncbi.nlm.nih.gov#" + xmlns:obo="http://purl.obolibrary.org/obo/" + xmlns:om="http://www.ontology-of-units-of-measure.org/resource/om-2/" + xmlns:prov="http://www.w3.org/ns/prov#" + xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" + xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" + xmlns:sbh="http://wiki.synbiohub.org/wiki/Terms/synbiohub#" + xmlns:sbol="http://sbols.org/v2#" + xmlns:sybio="http://www.sybio.ncl.ac.uk#" + xmlns:synbiohub="http://synbiohub.org#" + xmlns:sys-bio="http://sys-bio.org#" + xmlns:xsd="http://www.w3.org/2001/XMLSchema#dateTime/"> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_10000_cds_seq/1"> + <sbol:displayId>P21683_10000_cds_seq</sbol:displayId> + <sbol:elements>ATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_10000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_10000_gene_seq/1"> + <sbol:displayId>P21683_10000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGATCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCTCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_10000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_10000_rbs_seq/1"> + <sbol:displayId>P21683_10000_rbs_seq</sbol:displayId> + <sbol:elements>TCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAA</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_10000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_20000_cds_seq/1"> + <sbol:displayId>P21683_20000_cds_seq</sbol:displayId> + <sbol:elements>ATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_20000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_20000_gene_seq/1"> + <sbol:displayId>P21683_20000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGAATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_20000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_20000_rbs_seq/1"> + <sbol:displayId>P21683_20000_rbs_seq</sbol:displayId> + <sbol:elements>ATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_20000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_30000_cds_seq/1"> + <sbol:displayId>P21683_30000_cds_seq</sbol:displayId> + <sbol:elements>ATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_30000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_30000_gene_seq/1"> + <sbol:displayId>P21683_30000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGAGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_30000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21683_30000_rbs_seq/1"> + <sbol:displayId>P21683_30000_rbs_seq</sbol:displayId> + <sbol:elements>GGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21683_30000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_10000_cds_seq/1"> + <sbol:displayId>P21684_10000_cds_seq</sbol:displayId> + <sbol:elements>ATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_10000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_10000_gene_seq/1"> + <sbol:displayId>P21684_10000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGATTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGAATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCCTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGAATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCCAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_10000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_10000_rbs_seq/1"> + <sbol:displayId>P21684_10000_rbs_seq</sbol:displayId> + <sbol:elements>TTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGA</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_10000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_20000_cds_seq/1"> + <sbol:displayId>P21684_20000_cds_seq</sbol:displayId> + <sbol:elements>ATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_20000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_20000_gene_seq/1"> + <sbol:displayId>P21684_20000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGAACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTGATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCTTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTGATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCTAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_20000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_20000_rbs_seq/1"> + <sbol:displayId>P21684_20000_rbs_seq</sbol:displayId> + <sbol:elements>ACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_20000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_30000_cds_seq/1"> + <sbol:displayId>P21684_30000_cds_seq</sbol:displayId> + <sbol:elements>ATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_30000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_30000_gene_seq/1"> + <sbol:displayId>P21684_30000_gene_seq</sbol:displayId> + <sbol:elements>TGGGCCAGATATGATGTAGGAAGCGATTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCAATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGCTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCAATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGCAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_30000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21684_30000_rbs_seq/1"> + <sbol:displayId>P21684_30000_rbs_seq</sbol:displayId> + <sbol:elements>TTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCA</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21684_30000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_10000_cds_seq/1"> + <sbol:displayId>P21685_10000_cds_seq</sbol:displayId> + <sbol:elements>ATGAAGCCAACCACGGTAATTGGTGCCGGTTTTGGCGGGTTAGCCCTGGCCATCCGCCTGCAAGCGGCAGGGATTCCTGTGCTTTTGTTGGAACAGCGCGATAAGCCGGGCGGCCGTGCCTACGTTTACGAAGATCAGGGCTTCACCTTTGATGCGGGCCCGACGGTGATCACTGATCCTTCAGCAATCGAAGAACTCTTTGCCCTGGCAGGGAAACAACTTAAAGAGTATGTGGAATTGCTACCAGTCACCCCGTTTTACCGCCTGTGCTGGGAAAGCGGCAAAGTGTTTAATTATGATAACGATCAGACCCGCCTGGAGGCTCAGATTCAACAATTTAATCCGCGTGACGTTGAGGGGTATCGCCAGTTTCTGGATTATTCACGTGCGGTCTTCAAAGAGGGATATCTGAAACTCGGCACAGTTCCATTTCTGAGCTTTCGTGATATGCTCCGTGCAGCACCGCAGCTGGCAAAACTGCAGGCCTGGCGTAGCGTTTATAGCAAAGTCGCGTCGTATATCGAGGATGAGCACCTGCGCCAGGCATTTAGTTTTCACAGTCTGCTGGTGGGCGGCAATCCGTTCGCTACGAGTTCGATTTACACGTTGATTCATGCTCTGGAGCGTGAATGGGGCGTATGGTTCCCGCGCGGTGGCACCGGGGCGCTTGTACAGGGCATGATTAAACTCTTCCAGGATCTCGGTGGCGAAGTGGTGCTGAATGCGCGGGTGTCACACATGGAGACCACTGGTAATAAAATTGAAGCCGTACATTTAGAAGACGGCCGCAGGTTCCTGACACAGGCGGTAGCTTCTAACGCAGATGTTGTGCATACCTACCGAGACCTGCTATCACAGCATCCGGCGGCGGTGAAACAGAGCAACAAGCTCCAGACTAAACGTATGTCCAACAGCCTTTTCGTGCTGTACTTTGGGCTGAACCACCATCATGATCAGTTAGCACATCATACGGTGTGTTTCGGGCCACGGTACCGCGAACTTATTGACGAGATTTTTAATCACGATGGTCTAGCCGAAGATTTCTCTCTGTACCTGCACGCCCCGTGCGTGACCGATTCGAGCCTAGCTCCAGAAGGTTGCGGTAGTTATTACGTTCTCGCCCCGGTGCCCCATCTCGGGACTGCAAACCTGGATTGGACTGTAGAAGGCCCCAAGCTGCGCGACCGAATTTTTGCCTACCTTGAGCAGCACTACATGCCTGGCCTTCGATCTCAGTTAGTAACTCACCGCATGTTCACCCCATTCGACTTCCGCGATCAGCTGAACGCGTACCATGGCAGCGCGTTTAGCGTCGAACCGGTGCTTACGCAGAGCGCATGGTTTCGACCGCATAACCGAGATAAGACGATCACGAATCTGTATTTAGTAGGCGCGGGCACCCATCCTGGCGCGGGAATCCCAGGCGTGATCGGCAGTGCAAAAGCCACGGCGGGCCTGATGCTGGAGGATCTCATC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_10000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_10000_gene_seq/1"> + <sbol:displayId>P21685_10000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCCAGAGGGGCGTAATTACTGCGCCCCGAGTCATTAACTCAAACTGCGTGAGGGAGGTTATTATGAAGCCAACCACGGTAATTGGTGCCGGTTTTGGCGGGTTAGCCCTGGCCATCCGCCTGCAAGCGGCAGGGATTCCTGTGCTTTTGTTGGAACAGCGCGATAAGCCGGGCGGCCGTGCCTACGTTTACGAAGATCAGGGCTTCACCTTTGATGCGGGCCCGACGGTGATCACTGATCCTTCAGCAATCGAAGAACTCTTTGCCCTGGCAGGGAAACAACTTAAAGAGTATGTGGAATTGCTACCAGTCACCCCGTTTTACCGCCTGTGCTGGGAAAGCGGCAAAGTGTTTAATTATGATAACGATCAGACCCGCCTGGAGGCTCAGATTCAACAATTTAATCCGCGTGACGTTGAGGGGTATCGCCAGTTTCTGGATTATTCACGTGCGGTCTTCAAAGAGGGATATCTGAAACTCGGCACAGTTCCATTTCTGAGCTTTCGTGATATGCTCCGTGCAGCACCGCAGCTGGCAAAACTGCAGGCCTGGCGTAGCGTTTATAGCAAAGTCGCGTCGTATATCGAGGATGAGCACCTGCGCCAGGCATTTAGTTTTCACAGTCTGCTGGTGGGCGGCAATCCGTTCGCTACGAGTTCGATTTACACGTTGATTCATGCTCTGGAGCGTGAATGGGGCGTATGGTTCCCGCGCGGTGGCACCGGGGCGCTTGTACAGGGCATGATTAAACTCTTCCAGGATCTCGGTGGCGAAGTGGTGCTGAATGCGCGGGTGTCACACATGGAGACCACTGGTAATAAAATTGAAGCCGTACATTTAGAAGACGGCCGCAGGTTCCTGACACAGGCGGTAGCTTCTAACGCAGATGTTGTGCATACCTACCGAGACCTGCTATCACAGCATCCGGCGGCGGTGAAACAGAGCAACAAGCTCCAGACTAAACGTATGTCCAACAGCCTTTTCGTGCTGTACTTTGGGCTGAACCACCATCATGATCAGTTAGCACATCATACGGTGTGTTTCGGGCCACGGTACCGCGAACTTATTGACGAGATTTTTAATCACGATGGTCTAGCCGAAGATTTCTCTCTGTACCTGCACGCCCCGTGCGTGACCGATTCGAGCCTAGCTCCAGAAGGTTGCGGTAGTTATTACGTTCTCGCCCCGGTGCCCCATCTCGGGACTGCAAACCTGGATTGGACTGTAGAAGGCCCCAAGCTGCGCGACCGAATTTTTGCCTACCTTGAGCAGCACTACATGCCTGGCCTTCGATCTCAGTTAGTAACTCACCGCATGTTCACCCCATTCGACTTCCGCGATCAGCTGAACGCGTACCATGGCAGCGCGTTTAGCGTCGAACCGGTGCTTACGCAGAGCGCATGGTTTCGACCGCATAACCGAGATAAGACGATCACGAATCTGTATTTAGTAGGCGCGGGCACCCATCCTGGCGCGGGAATCCCAGGCGTGATCGGCAGTGCAAAAGCCACGGCGGGCCTGATGCTGGAGGATCTCATCTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_10000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_10000_rbs_seq/1"> + <sbol:displayId>P21685_10000_rbs_seq</sbol:displayId> + <sbol:elements>CAGAGGGGCGTAATTACTGCGCCCCGAGTCATTAACTCAAACTGCGTGAGGGAGGTTATT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_10000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_20000_cds_seq/1"> + <sbol:displayId>P21685_20000_cds_seq</sbol:displayId> + <sbol:elements>ATGAAACCAACGACCGTAATCGGCGCCGGCTTTGGGGGTTTGGCGCTTGCGATTCGCCTGCAGGCAGCGGGGATTCCGGTCTTGCTGCTCGAGCAGCGTGATAAACCGGGCGGCCGCGCTTATGTATATGAAGATCAGGGCTTTACCTTTGATGCAGGCCCGACGGTGATTACGGATCCGAGCGCGATAGAAGAACTTTTCGCCCTGGCCGGCAAACAACTGAAAGAGTATGTGGAGTTGCTGCCCGTAACCCCTTTCTATCGGTTGTGCTGGGAATCCGGTAAGGTGTTCAATTATGACAACGATCAAACCCGTCTGGAAGCCCAGATTCAACAGTTTAACCCGCGTGACGTTGAAGGCTATCGGCAGTTCCTTGACTACAGTCGCGCTGTCTTTAAAGAGGGTTACTTAAAACTGGGCACCGTGCCGTTCTTGTCGTTCCGCGATATGCTACGCGCAGCGCCTCAATTAGCCAAGCTGCAGGCATGGCGTTCCGTGTATAGCAAAGTCGCGAGTTATATTGAGGATGAACACCTGCGCCAGGCCTTTTCTTTTCATAGTCTGTTGGTAGGTGGCAACCCGTTCGCCACGTCCTCAATCTACACCCTGATCCATGCGCTGGAGCGTGAATGGGGCGTGTGGTTCCCGCGCGGGGGCACGGGGGCCCTGGTGCAGGGCATGATCAAACTGTTCCAGGACCTGGGCGGAGAAGTGGTTTTGAATGCCCGCGTGTCGCATATGGAAACGACAGGCAATAAAATTGAAGCGGTGCATTTAGAAGACGGCCGTCGCTTTCTGACCCAGGCTGTTGCCTCGAACGCGGATGTGGTACACACCTATCGGGATCTGCTCAGCCAGCATCCGGCAGCCGTAAAACAGTCTAACAAACTGCAAACGAAACGTATGAGCAACTCACTGTTCGTTCTATATTTTGGCCTGAACCACCACCACGACCAACTGGCACATCACACGGTTTGTTTTGGGCCGCGGTATCGCGAATTAATTGATGAAATTTTTAATCATGACGGGTTGGCGGAAGATTTTTCCCTGTATTTGCACGCGCCGTGTGTGACCGATAGCAGTTTGGCCCCGGAAGGTTGCGGGAGCTACTATGTACTGGCGCCGGTGCCTCATCTTGGAACCGCAAACCTGGACTGGACCGTGGAAGGCCCAAAACTCCGCGACCGTATATTTGCGTATCTGGAACAACACTACATGCCCGGCCTCCGCAGTCAACTGGTGACTCACCGCATGTTTACCCCGTTTGATTTTCGTGATCAGCTGAATGCTTACCATGGAAGTGCATTTAGCGTGGAACCAGTACTGACACAATCCGCCTGGTTCCGCCCGCATAATAGGGATAAAACCATAACCAATCTCTATCTGGTGGGTGCTGGCACTCATCCGGGCGCTGGTATTCCGGGCGTAATCGGTAGCGCCAAAGCTACCGCAGGGTTGATGCTTGAGGATCTGATT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_20000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_20000_gene_seq/1"> + <sbol:displayId>P21685_20000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCTCCGGATTCGTCACGCTCGTTCGTGCTGGTAGACTTTTATAACAGGAAGGAGGAAGACACATGAAACCAACGACCGTAATCGGCGCCGGCTTTGGGGGTTTGGCGCTTGCGATTCGCCTGCAGGCAGCGGGGATTCCGGTCTTGCTGCTCGAGCAGCGTGATAAACCGGGCGGCCGCGCTTATGTATATGAAGATCAGGGCTTTACCTTTGATGCAGGCCCGACGGTGATTACGGATCCGAGCGCGATAGAAGAACTTTTCGCCCTGGCCGGCAAACAACTGAAAGAGTATGTGGAGTTGCTGCCCGTAACCCCTTTCTATCGGTTGTGCTGGGAATCCGGTAAGGTGTTCAATTATGACAACGATCAAACCCGTCTGGAAGCCCAGATTCAACAGTTTAACCCGCGTGACGTTGAAGGCTATCGGCAGTTCCTTGACTACAGTCGCGCTGTCTTTAAAGAGGGTTACTTAAAACTGGGCACCGTGCCGTTCTTGTCGTTCCGCGATATGCTACGCGCAGCGCCTCAATTAGCCAAGCTGCAGGCATGGCGTTCCGTGTATAGCAAAGTCGCGAGTTATATTGAGGATGAACACCTGCGCCAGGCCTTTTCTTTTCATAGTCTGTTGGTAGGTGGCAACCCGTTCGCCACGTCCTCAATCTACACCCTGATCCATGCGCTGGAGCGTGAATGGGGCGTGTGGTTCCCGCGCGGGGGCACGGGGGCCCTGGTGCAGGGCATGATCAAACTGTTCCAGGACCTGGGCGGAGAAGTGGTTTTGAATGCCCGCGTGTCGCATATGGAAACGACAGGCAATAAAATTGAAGCGGTGCATTTAGAAGACGGCCGTCGCTTTCTGACCCAGGCTGTTGCCTCGAACGCGGATGTGGTACACACCTATCGGGATCTGCTCAGCCAGCATCCGGCAGCCGTAAAACAGTCTAACAAACTGCAAACGAAACGTATGAGCAACTCACTGTTCGTTCTATATTTTGGCCTGAACCACCACCACGACCAACTGGCACATCACACGGTTTGTTTTGGGCCGCGGTATCGCGAATTAATTGATGAAATTTTTAATCATGACGGGTTGGCGGAAGATTTTTCCCTGTATTTGCACGCGCCGTGTGTGACCGATAGCAGTTTGGCCCCGGAAGGTTGCGGGAGCTACTATGTACTGGCGCCGGTGCCTCATCTTGGAACCGCAAACCTGGACTGGACCGTGGAAGGCCCAAAACTCCGCGACCGTATATTTGCGTATCTGGAACAACACTACATGCCCGGCCTCCGCAGTCAACTGGTGACTCACCGCATGTTTACCCCGTTTGATTTTCGTGATCAGCTGAATGCTTACCATGGAAGTGCATTTAGCGTGGAACCAGTACTGACACAATCCGCCTGGTTCCGCCCGCATAATAGGGATAAAACCATAACCAATCTCTATCTGGTGGGTGCTGGCACTCATCCGGGCGCTGGTATTCCGGGCGTAATCGGTAGCGCCAAAGCTACCGCAGGGTTGATGCTTGAGGATCTGATTTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_20000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_20000_rbs_seq/1"> + <sbol:displayId>P21685_20000_rbs_seq</sbol:displayId> + <sbol:elements>TCCGGATTCGTCACGCTCGTTCGTGCTGGTAGACTTTTATAACAGGAAGGAGGAAGACAC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_20000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_30000_cds_seq/1"> + <sbol:displayId>P21685_30000_cds_seq</sbol:displayId> + <sbol:elements>ATGAAACCGACTACGGTGATTGGCGCAGGTTTTGGCGGCTTGGCGCTTGCGATTCGGTTACAGGCCGCGGGCATCCCGGTACTGTTGCTCGAACAACGTGATAAACCTGGTGGGCGAGCGTACGTTTACGAGGATCAAGGCTTTACTTTTGATGCCGGTCCTACAGTAATTACTGATCCGAGCGCAATTGAAGAATTATTTGCCCTTGCAGGCAAACAGCTGAAAGAATATGTCGAGCTTCTCCCGGTGACGCCGTTTTATCGCCTGTGTTGGGAGAGCGGCAAAGTCTTCAACTACGACAATGATCAGACCCGTCTGGAAGCTCAGATTCAGCAGTTTAACCCGAGGGATGTGGAGGGCTACCGACAGTTTCTGGATTACAGTCGCGCCGTGTTTAAAGAAGGCTACCTGAAACTGGGGACTGTCCCTTTCCTGTCGTTCCGTGATATGCTGCGCGCGGCGCCTCAGTTAGCTAAGCTGCAAGCGTGGCGCTCGGTTTATTCTAAAGTAGCATCATATATCGAAGATGAACATCTGCGCCAGGCGTTTTCTTTTCATTCCCTGCTGGTCGGGGGTAATCCGTTTGCAACCTCCTCGATTTACACCCTGATCCATGCGTTAGAACGCGAATGGGGCGTATGGTTCCCACGCGGCGGCACAGGTGCCCTCGTACAGGGTATGATCAAACTCTTTCAGGATCTGGGCGGTGAAGTTGTGCTGAATGCGAGGGTATCACACATGGAAACGACCGGTAACAAAATCGAAGCCGTCCACCTGGAGGATGGACGCCGTTTTCTCACTCAGGCTGTGGCCAGTAACGCGGACGTTGTTCATACGTATCGTGATTTACTTAGCCAGCACCCGGCGGCAGTGAAACAGAGCAACAAATTGCAGACCAAGCGCATGTCTAACTCCCTGTTTGTGCTGTATTTTGGGCTTAACCACCACCACGATCAATTAGCGCACCACACCGTTTGCTTCGGCCCGCGCTACCGCGAGCTGATCGACGAGATTTTTAACCACGATGGATTAGCGGAAGACTTCTCTTTATATCTACATGCCCCGTGTGTGACCGACTCATCGCTCGCCCCGGAGGGTTGCGGTAGTTATTACGTTCTGGCGCCGGTGCCACACCTGGGCACCGCGAATTTGGACTGGACCGTGGAAGGTCCGAAGCTGCGCGATCGTATCTTCGCGTACCTGGAACAGCATTACATGCCAGGCCTGCGCTCCCAATTAGTTACCCATCGTATGTTCACGCCTTTTGATTTTCGCGACCAATTGAACGCCTACCATGGTTCGGCATTCAGCGTAGAACCGGTTCTGACGCAGTCGGCGTGGTTCCGCCCGCATAACCGTGATAAAACCATTACAAACCTGTACCTGGTGGGAGCAGGTACACATCCTGGTGCCGGCATTCCAGGTGTGATTGGTAGCGCGAAAGCTACCGCAGGCCTCATGTTAGAAGATCTGATT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_30000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_30000_gene_seq/1"> + <sbol:displayId>P21685_30000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCTTAGCTAGATCGGTAAAATTTCCGCTGGGATACGGAGGGGAATGAAGTGAGGGGGAATAAATGAAACCGACTACGGTGATTGGCGCAGGTTTTGGCGGCTTGGCGCTTGCGATTCGGTTACAGGCCGCGGGCATCCCGGTACTGTTGCTCGAACAACGTGATAAACCTGGTGGGCGAGCGTACGTTTACGAGGATCAAGGCTTTACTTTTGATGCCGGTCCTACAGTAATTACTGATCCGAGCGCAATTGAAGAATTATTTGCCCTTGCAGGCAAACAGCTGAAAGAATATGTCGAGCTTCTCCCGGTGACGCCGTTTTATCGCCTGTGTTGGGAGAGCGGCAAAGTCTTCAACTACGACAATGATCAGACCCGTCTGGAAGCTCAGATTCAGCAGTTTAACCCGAGGGATGTGGAGGGCTACCGACAGTTTCTGGATTACAGTCGCGCCGTGTTTAAAGAAGGCTACCTGAAACTGGGGACTGTCCCTTTCCTGTCGTTCCGTGATATGCTGCGCGCGGCGCCTCAGTTAGCTAAGCTGCAAGCGTGGCGCTCGGTTTATTCTAAAGTAGCATCATATATCGAAGATGAACATCTGCGCCAGGCGTTTTCTTTTCATTCCCTGCTGGTCGGGGGTAATCCGTTTGCAACCTCCTCGATTTACACCCTGATCCATGCGTTAGAACGCGAATGGGGCGTATGGTTCCCACGCGGCGGCACAGGTGCCCTCGTACAGGGTATGATCAAACTCTTTCAGGATCTGGGCGGTGAAGTTGTGCTGAATGCGAGGGTATCACACATGGAAACGACCGGTAACAAAATCGAAGCCGTCCACCTGGAGGATGGACGCCGTTTTCTCACTCAGGCTGTGGCCAGTAACGCGGACGTTGTTCATACGTATCGTGATTTACTTAGCCAGCACCCGGCGGCAGTGAAACAGAGCAACAAATTGCAGACCAAGCGCATGTCTAACTCCCTGTTTGTGCTGTATTTTGGGCTTAACCACCACCACGATCAATTAGCGCACCACACCGTTTGCTTCGGCCCGCGCTACCGCGAGCTGATCGACGAGATTTTTAACCACGATGGATTAGCGGAAGACTTCTCTTTATATCTACATGCCCCGTGTGTGACCGACTCATCGCTCGCCCCGGAGGGTTGCGGTAGTTATTACGTTCTGGCGCCGGTGCCACACCTGGGCACCGCGAATTTGGACTGGACCGTGGAAGGTCCGAAGCTGCGCGATCGTATCTTCGCGTACCTGGAACAGCATTACATGCCAGGCCTGCGCTCCCAATTAGTTACCCATCGTATGTTCACGCCTTTTGATTTTCGCGACCAATTGAACGCCTACCATGGTTCGGCATTCAGCGTAGAACCGGTTCTGACGCAGTCGGCGTGGTTCCGCCCGCATAACCGTGATAAAACCATTACAAACCTGTACCTGGTGGGAGCAGGTACACATCCTGGTGCCGGCATTCCAGGTGTGATTGGTAGCGCGAAAGCTACCGCAGGCCTCATGTTAGAAGATCTGATTTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_30000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21685_30000_rbs_seq/1"> + <sbol:displayId>P21685_30000_rbs_seq</sbol:displayId> + <sbol:elements>TTAGCTAGATCGGTAAAATTTCCGCTGGGATACGGAGGGGAATGAAGTGAGGGGGAATAA</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21685_30000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_10000_cds_seq/1"> + <sbol:displayId>P21687_10000_cds_seq</sbol:displayId> + <sbol:elements>ATGCAGCCGCACTATGATCTGATTCTGGTTGGAGCTGGTCTGGCGAACGGCCTAATAGCGCTGCGCCTCCAGCAACAGCAGCCGGACATGCGTATTCTTCTTATTGACGCCGCACCGCAGGCGGGTGGGAATCACACCTGGTCATTCCACCACGATGATCTGACCGAAAGTCAACATCGGTGGATCGCTCCGCTTGTTGTGCATCACTGGCCGGATTATCAGGTGCGTTTTCCGACCCGCCGTCGGAAACTGAATTCTGGATATTTCTGTATTACTAGTCAGCGTTTCGCCGAAGTCTTACAGCGTCAGTTCGGCCCACATCTGTGGATGGATACGGCCGTCGCGGAAGTCAATGCGGAATCGGTACGCCTGAAGAAGGGTCAGGTAATCGGAGCACGAGCGGTAATCGATGGGCGGGGCTATGCGGCAAACTCAGCTTTATCAGTTGGATTTCAAGCGTTTATTGGCCAAGAATGGCGTTTGAGTCACCCGCACGGCCTGAGCTCACCTATTATTATGGACGCAACTGTTGACCAGCAGAACGGTTACCGCTTTGTGTACTCACTGCCACTCTCACCTACCCGCCTCCTGATTGAAGATACTCATTATATTGACAATGCCACTTTGGACCCCGAGTGCGCGCGTCAGAATATCTGCGATTATGCAGCACAGCAAGGCTGGCAATTACAGACTCTGCTGCGCGAGGAACAAGGCGCGCTTCCGATTACCCTTAGTGGTAACGCGGATGCTTTCTGGCAGCAACGCCCGCTGGCGTGTTCAGGTCTGCGCGCGGGCCTGTTCCACCCGACAACCGGATATTCTCTGCCACTGGCAGTTGCGGTGGCGGATCGCCTCAGCGCTTTAGATGTGTTTACGTCAGCCAGCATACATCACGCAATTACCCACTTCGCGCGTGAAAGGTGGCAGCAACAGGGATTCTTCCGCATGTTAAATCGTATGCTATTTCTAGCCGGCCCAGCGGATTCAAGATGGCGTGTGATGCAGCGGTTCTATGGTCTGCCCGAGGACCTCATCGCGCGCTTCTATGCCGGTAAACTCACCCTGACTGATCGCTTACGCATTTTATCGGGGAAACCTCCCGTTCCGGTGCTAGCCGCACTTCAGGCGATTATGACGACCCACCGT</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_10000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_10000_gene_seq/1"> + <sbol:displayId>P21687_10000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCGCGACAAACGAACAACTCAGACACTTCGGTGATTGACTGTTATCTTGAAGGGGGCCTACAATGCAGCCGCACTATGATCTGATTCTGGTTGGAGCTGGTCTGGCGAACGGCCTAATAGCGCTGCGCCTCCAGCAACAGCAGCCGGACATGCGTATTCTTCTTATTGACGCCGCACCGCAGGCGGGTGGGAATCACACCTGGTCATTCCACCACGATGATCTGACCGAAAGTCAACATCGGTGGATCGCTCCGCTTGTTGTGCATCACTGGCCGGATTATCAGGTGCGTTTTCCGACCCGCCGTCGGAAACTGAATTCTGGATATTTCTGTATTACTAGTCAGCGTTTCGCCGAAGTCTTACAGCGTCAGTTCGGCCCACATCTGTGGATGGATACGGCCGTCGCGGAAGTCAATGCGGAATCGGTACGCCTGAAGAAGGGTCAGGTAATCGGAGCACGAGCGGTAATCGATGGGCGGGGCTATGCGGCAAACTCAGCTTTATCAGTTGGATTTCAAGCGTTTATTGGCCAAGAATGGCGTTTGAGTCACCCGCACGGCCTGAGCTCACCTATTATTATGGACGCAACTGTTGACCAGCAGAACGGTTACCGCTTTGTGTACTCACTGCCACTCTCACCTACCCGCCTCCTGATTGAAGATACTCATTATATTGACAATGCCACTTTGGACCCCGAGTGCGCGCGTCAGAATATCTGCGATTATGCAGCACAGCAAGGCTGGCAATTACAGACTCTGCTGCGCGAGGAACAAGGCGCGCTTCCGATTACCCTTAGTGGTAACGCGGATGCTTTCTGGCAGCAACGCCCGCTGGCGTGTTCAGGTCTGCGCGCGGGCCTGTTCCACCCGACAACCGGATATTCTCTGCCACTGGCAGTTGCGGTGGCGGATCGCCTCAGCGCTTTAGATGTGTTTACGTCAGCCAGCATACATCACGCAATTACCCACTTCGCGCGTGAAAGGTGGCAGCAACAGGGATTCTTCCGCATGTTAAATCGTATGCTATTTCTAGCCGGCCCAGCGGATTCAAGATGGCGTGTGATGCAGCGGTTCTATGGTCTGCCCGAGGACCTCATCGCGCGCTTCTATGCCGGTAAACTCACCCTGACTGATCGCTTACGCATTTTATCGGGGAAACCTCCCGTTCCGGTGCTAGCCGCACTTCAGGCGATTATGACGACCCACCGTTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_10000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_10000_rbs_seq/1"> + <sbol:displayId>P21687_10000_rbs_seq</sbol:displayId> + <sbol:elements>GCGACAAACGAACAACTCAGACACTTCGGTGATTGACTGTTATCTTGAAGGGGGCCTACA</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_10000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_20000_cds_seq/1"> + <sbol:displayId>P21687_20000_cds_seq</sbol:displayId> + <sbol:elements>ATGCAGCCGCATTACGACTTGATTTTGGTGGGCGCCGGCCTGGCAAACGGCCTCATTGCCTTACGCTTACAGCAGCAGCAACCGGATATGCGCATCCTGCTCATTGATGCGGCCCCGCAGGCAGGTGGTAATCATACCTGGAGCTTTCATCATGATGATTTGACCGAATCGCAGCATCGTTGGATTGCGCCGTTAGTTGTTCATCACTGGCCAGACTATCAGGTGCGCTTCCCCACCCGACGCCGTAAACTGAACTCTGGCTATTTTTGCATTACCTCCCAGCGCTTTGCAGAAGTGCTGCAGCGCCAATTCGGCCCCCATTTGTGGATGGATACCGCCGTAGCCGAAGTAAATGCCGAATCCGTGCGGCTGAAGAAAGGTCAGGTCATTGGGGCTCGCGCGGTGATTGATGGGCGCGGATATGCGGCCAACAGTGCGCTGTCAGTGGGCTTTCAGGCCTTTATTGGCCAGGAATGGCGCCTTAGCCATCCCCACGGTCTCAGTTCCCCGATCATTATGGACGCCACTGTGGACCAACAAAACGGCTACCGTTTCGTGTACTCGCTCCCGCTTAGCCCGACCCGACTCTTGATCGAAGATACGCACTATATCGACAACGCCACCCTGGATCCGGAGTGTGCTCGTCAGAATATCTGTGACTACGCAGCGCAACAGGGTTGGCAGCTCCAAACCCTGCTACGCGAAGAACAGGGCGCACTGCCTATTACACTGTCCGGTAACGCGGACGCCTTTTGGCAGCAGCGCCCTCTGGCGTGTAGTGGCCTGCGTGCGGGCCTGTTTCATCCCACGACTGGATATAGTTTGCCGCTCGCGGTTGCGGTGGCGGATCGTCTCAGCGCACTTGATGTGTTTACTAGCGCAAGTATTCACCACGCGATTACCCATTTTGCGCGCGAACGTTGGCAGCAGCAAGGCTTCTTTCGTATGCTCAACCGCATGTTGTTCTTGGCGGGTCCAGCAGATTCGCGCTGGCGTGTGATGCAGCGTTTTTACGGGCTTCCGGAGGATCTCATTGCTCGCTTCTATGCGGGGAAACTGACCTTAACCGACCGACTGCGCATCCTGTCAGGAAAACCGCCTGTGCCGGTCTTAGCAGCGCTGCAAGCTATTATGACTACCCATCGC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_20000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_20000_gene_seq/1"> + <sbol:displayId>P21687_20000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCCCACCCAATGGTAACCCAAAAATTCTCGATCCTCACGGATACGGAAGCAAAGGAGGTATCATGCAGCCGCATTACGACTTGATTTTGGTGGGCGCCGGCCTGGCAAACGGCCTCATTGCCTTACGCTTACAGCAGCAGCAACCGGATATGCGCATCCTGCTCATTGATGCGGCCCCGCAGGCAGGTGGTAATCATACCTGGAGCTTTCATCATGATGATTTGACCGAATCGCAGCATCGTTGGATTGCGCCGTTAGTTGTTCATCACTGGCCAGACTATCAGGTGCGCTTCCCCACCCGACGCCGTAAACTGAACTCTGGCTATTTTTGCATTACCTCCCAGCGCTTTGCAGAAGTGCTGCAGCGCCAATTCGGCCCCCATTTGTGGATGGATACCGCCGTAGCCGAAGTAAATGCCGAATCCGTGCGGCTGAAGAAAGGTCAGGTCATTGGGGCTCGCGCGGTGATTGATGGGCGCGGATATGCGGCCAACAGTGCGCTGTCAGTGGGCTTTCAGGCCTTTATTGGCCAGGAATGGCGCCTTAGCCATCCCCACGGTCTCAGTTCCCCGATCATTATGGACGCCACTGTGGACCAACAAAACGGCTACCGTTTCGTGTACTCGCTCCCGCTTAGCCCGACCCGACTCTTGATCGAAGATACGCACTATATCGACAACGCCACCCTGGATCCGGAGTGTGCTCGTCAGAATATCTGTGACTACGCAGCGCAACAGGGTTGGCAGCTCCAAACCCTGCTACGCGAAGAACAGGGCGCACTGCCTATTACACTGTCCGGTAACGCGGACGCCTTTTGGCAGCAGCGCCCTCTGGCGTGTAGTGGCCTGCGTGCGGGCCTGTTTCATCCCACGACTGGATATAGTTTGCCGCTCGCGGTTGCGGTGGCGGATCGTCTCAGCGCACTTGATGTGTTTACTAGCGCAAGTATTCACCACGCGATTACCCATTTTGCGCGCGAACGTTGGCAGCAGCAAGGCTTCTTTCGTATGCTCAACCGCATGTTGTTCTTGGCGGGTCCAGCAGATTCGCGCTGGCGTGTGATGCAGCGTTTTTACGGGCTTCCGGAGGATCTCATTGCTCGCTTCTATGCGGGGAAACTGACCTTAACCGACCGACTGCGCATCCTGTCAGGAAAACCGCCTGTGCCGGTCTTAGCAGCGCTGCAAGCTATTATGACTACCCATCGCTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_20000_gene_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_20000_rbs_seq/1"> + <sbol:displayId>P21687_20000_rbs_seq</sbol:displayId> + <sbol:elements>CCACCCAATGGTAACCCAAAAATTCTCGATCCTCACGGATACGGAAGCAAAGGAGGTATC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_20000_rbs_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_30000_cds_seq/1"> + <sbol:displayId>P21687_30000_cds_seq</sbol:displayId> + <sbol:elements>ATGCAGCCTCATTACGATTTGATTCTTGTCGGTGCAGGTCTGGCGAACGGCTTAATTGCACTGCGCCTGCAGCAGCAGCAACCGGACATGAGGATTCTTCTTATTGATGCCGCGCCGCAAGCGGGTGGAAATCATACTTGGAGCTTTCACCACGATGACCTGACCGAATCGCAACATCGTTGGATTGCTCCGTTGGTGGTGCATCACTGGCCGGATTATCAAGTTCGCTTTCCCACCCGTCGTCGTAAATTGAACTCCGGTTACTTTTGTATCACGAGTCAACGTTTTGCTGAAGTCTTGCAGCGTCAATTCGGGCCGCATCTGTGGATGGATACTGCAGTGGCGGAAGTTAATGCCGAGTCAGTGCGGCTGAAGAAGGGCCAGGTCATCGGAGCACGTGCGGTCATTGATGGTCGCGGATATGCGGCGAATTCCGCACTGAGCGTAGGCTTCCAGGCGTTCATTGGCCAGGAATGGCGTCTGAGCCATCCCCACGGCTTAAGCAGCCCGATTATCATGGACGCGACCGTAGATCAGCAAAACGGTTACCGTTTCGTGTACTCATTGCCTTTATCCCCGACCCGTCTGTTAATTGAGGACACCCACTACATCGATAATGCGACCCTGGATCCGGAGTGTGCGCGTCAGAACATTTGTGACTACGCGGCCCAGCAGGGATGGCAGCTGCAAACGTTACTGCGTGAAGAACAAGGTGCGTTGCCGATCACACTTAGCGGCAATGCGGATGCGTTTTGGCAGCAGAGACCGCTGGCTTGTAGCGGTCTGCGCGCAGGTTTATTTCACCCCACCACGGGATATTCTCTCCCGTTGGCGGTAGCGGTTGCCGATAGGCTGAGCGCGTTAGACGTTTTTACGAGTGCGTCGATTCACCATGCGATTACTCACTTCGCCCGTGAACGCTGGCAGCAGCAGGGCTTCTTCCGCATGCTCAATCGGATGCTGTTTCTCGCTGGTCCGGCAGATTCGCGCTGGCGTGTGATGCAACGCTTTTACGGCTTACCTGAGGATTTAATTGCTCGTTTCTATGCTGGCAAACTAACTTTAACGGATCGCTTACGCATTCTGTCGGGCAAACCGCCCGTTCCGGTGCTTGCCGCCCTGCAAGCAATCATGACCACCCATCGC</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="http://examples.org/Sequence/P21687_30000_cds_seq"/> + <sbol:version>1</sbol:version> + </sbol:Sequence> + <sbol:Sequence rdf:about="http://examples.org/Sequence/P21687_30000_gene_seq/1"> + <sbol:displayId>P21687_30000_gene_seq</sbol:displayId> + <sbol:elements>GGGACATGGGGATCGATCACCAATAGCCTCCTGAATTCTAAGTCCTTTCCTGAATATGCCTACATAACGCTTAGAGGGAGGTAGAAAATGCAGCCTCATTACGATTTGATTCTTGTCGGTGCAGGTCTGGCGAACGGCTTAATTGCACTGCGCCTGCAGCAGCAGCAACCGGACATGAGGATTCTTCTTATTGATGCCGCGCCGCAAGCGGGTGGAAATCATACTTGGAGCTTTCACCACGATGACCTGACCGAATCGCAACATCGTTGGATTGCTCCGTTGGTGGTGCATCACTGGCCGGATTATCAAGTTCGCTTTCCCACCCGTCGTCGTAAATTGAACTCCGGTTACTTTTGTATCACGAGTCAACGTTTTGCTGAAGTCTTGCAGCGTCAATTCGGGCCGCATCTGTGGATGGATACTGCAGTGGCGGAAGTTAATGCCGAGTCAGTGCGGCTGAAGAAGGGCCAGGTCATCGGAGCACGTGCGGTCATTGATGGTCGCGGATATGCGGCGAATTCCGCACTGAGCGTAGGCTTCCAGGCGTTCATTGGCCAGGAATGGCGTCTGAGCCATCCCCACGGCTTAAGCAGCCCGATTATCATGGACGCGACCGTAGATCAGCAAAACGGTTACCGTTTCGTGTACTCATTGCCTTTATCCCCGACCCGTCTGTTAATTGAGGACACCCACTACATCGATAATGCGACCCTGGATCCGGAGTGTGCGCGTCAGAACATTTGTGACTACGCGGCCCAGCAGGGATGGCAGCTGCAAACGTTACTGCGTGAAGAACAAGGTGCGTTGCCGATCACACTTAGCGGCAATGCGGATGCGTTTTGGCAGCAGAGACCGCTGGCTTGTAGCGGTCTGCGCGCAGGTTTATTTCACCCCACCACGGGATATTCTCTCCCGTTGGCGGTAGCGGTTGCCGATAGGCTGAGCGCGTTAGACGTTTTTACGAGTGCGTCGATTCACCATGCGATTACTCACTTCGCCCGTGAACGCTGGCAGCAGCAGGGCTTCTTCCGCATGCTCAATCGGATGCTGTTTCTCGCTGGTCCGGCAGATTCGCGCTGGCGTGTGATGCAACGCTTTTACGGCTTACCTGAGGATTTAATTGCTCGTTTCTATGCTGGCAAACTAACTTTAACGGATCGCTTACGCATTCTGTCGGGCAAACCGCCCGTTCCGGTGCTTGCCGCCCTGCAAGCAATCATGACCACCCATCGCTACGCCTGGAAAACATTACTGACCCCC</sbol:elements> 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<sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_2/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_2</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_2"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_2/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_2</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_2"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J64101_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/1"/> + <sbol:displayId>BBa_J64101_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J64101_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_20000_gene/1"/> + <sbol:displayId>P21684_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_20000_gene/1"/> + <sbol:displayId>P21684_20000_gene_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21685_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21685_20000_gene/1"/> + <sbol:displayId>P21685_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21685_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid03</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J64101_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_2/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_2/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_3/1"> + <sbol:displayId>constraint_3</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_2/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_3"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_4/1"> + <sbol:displayId>constraint_4</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_2/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_6/1"> + <sbol:displayId>constraint_6</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_6"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21684_20000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_7/1"> + <sbol:displayId>constraint_7</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_7"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_8/1"> + <sbol:displayId>constraint_8</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21685_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_8"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_9/1"> + <sbol:displayId>constraint_9</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/constraint_9"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid03/P21685_20000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:type rdf:resource="http://identifiers.org/so/SO:0000987"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/1"> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_B1006_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_J64101_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/1"/> + <sbol:displayId>BBa_J64101_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_J64101_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_K1847014_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid04/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid04/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + 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<sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_6"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/P21683_30000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_7/1"> + <sbol:displayId>constraint_7</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_7"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/BBa_B1006_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_8/1"> + <sbol:displayId>constraint_8</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/P21687_30000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_8"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_9/1"> + <sbol:displayId>constraint_9</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/constraint_9"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid13/P21687_30000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:type rdf:resource="http://identifiers.org/so/SO:0000987"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/1"> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J56012_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J56012_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J64101_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/1"/> + <sbol:displayId>BBa_J64101_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J64101_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_K1847014_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21684_30000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_30000_gene/1"/> + <sbol:displayId>P21684_30000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21684_30000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21687_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21687_10000_gene/1"/> + <sbol:displayId>P21687_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21687_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid14</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J64101_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_K1847014_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21683_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_K1847014_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_3/1"> + <sbol:displayId>constraint_3</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21684_30000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_3"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21683_20000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_4/1"> + <sbol:displayId>constraint_4</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21684_30000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_6/1"> + <sbol:displayId>constraint_6</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21687_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_6"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_7/1"> + <sbol:displayId>constraint_7</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/constraint_7"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid14/P21687_10000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:type rdf:resource="http://identifiers.org/so/SO:0000987"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition 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rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_J56012_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_0</sbol:displayId> + 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<sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_K1847014_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_K1847014_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component 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rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/P21683_10000_gene_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid16</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_J64101_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_K1847014_1/1"/> + 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rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_K1847014_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/BBa_B1006_2/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/P21683_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid16/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject 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rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid22/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid22/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid22/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid22/BBa_I50032_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid22/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid22/BBa_K1847014_0/1"/> + 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<sbol:displayId>P21683_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21683_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21684_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_10000_gene/1"/> + <sbol:displayId>P21684_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21684_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21685_30000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21685_30000_gene/1"/> + <sbol:displayId>P21685_30000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21685_30000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid23</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_I50041_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_J56012_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21683_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_J56012_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_3/1"> + <sbol:displayId>constraint_3</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21684_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_3"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21683_10000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_4/1"> + <sbol:displayId>constraint_4</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_B1006_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21684_10000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_B1006_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_6/1"> + <sbol:displayId>constraint_6</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21685_30000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_6"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_7/1"> + <sbol:displayId>constraint_7</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/constraint_7"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid23/P21685_30000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:type rdf:resource="http://identifiers.org/so/SO:0000987"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/1"> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_B1006_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I50041_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/1"/> + <sbol:displayId>BBa_I50041_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I50041_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_K1847014_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_10000_gene/1"/> + <sbol:displayId>P21684_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_20000_gene/1"/> + <sbol:displayId>P21684_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid24</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I50041_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_K1847014_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_K1847014_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_3/1"> + <sbol:displayId>constraint_3</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_3"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_20000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_4/1"> + <sbol:displayId>constraint_4</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21683_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21684_10000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid24/P21683_20000_gene_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:type rdf:resource="http://identifiers.org/so/SO:0000987"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/1"> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_B1006_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_B1006_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_B1006_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I50032_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/1"/> + <sbol:displayId>BBa_I50032_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I50032_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_J56012_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_J56012_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_K1847014_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21684_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_10000_gene/1"/> + <sbol:displayId>P21684_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21684_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21684_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_20000_gene/1"/> + <sbol:displayId>P21684_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21684_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid25</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I50032_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21684_10000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_3/1"> + 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<sbol:displayId>constraint_6</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/P21683_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_6"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_K1847014_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_7/1"> + <sbol:displayId>constraint_7</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/BBa_B1006_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid25/constraint_7"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + 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+ <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_B1006_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_B1006_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I13800_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/1"/> + <sbol:displayId>BBa_I13800_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I13800_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I50032_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/1"/> + <sbol:displayId>BBa_I50032_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I50032_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_J56012/1"/> + <sbol:displayId>BBa_J56012_1</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_1"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21683_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_10000_gene/1"/> + <sbol:displayId>P21683_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21683_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21684_30000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_30000_gene/1"/> + <sbol:displayId>P21684_30000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21684_30000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid26</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_I50032_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_1/1"/> + 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rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_4"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/BBa_B1006_1/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_5/1"> + <sbol:displayId>constraint_5</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/P21684_30000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid26/constraint_5"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject 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rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid27/BBa_I50032_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid27/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid27/BBa_K1847014_1/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid27/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid27/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid27/constraint_2/1"> + 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rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/1"> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_B1006_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/1"/> + <sbol:displayId>BBa_B1006_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_B1006_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_B1006_1/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition 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rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_K1847014_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/1"/> + <sbol:displayId>BBa_K1847014_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_K1847014_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21683_10000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_10000_gene/1"/> + <sbol:displayId>P21683_10000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21683_10000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21683_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21683_20000_gene/1"/> + <sbol:displayId>P21683_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21683_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:component> + <sbol:Component rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21685_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21685_20000_gene/1"/> + <sbol:displayId>P21685_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21685_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid28</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_I50041_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_J56012_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/P21683_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid28/BBa_J56012_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid28/constraint_3/1"> + 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rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid30/P21684_20000_gene_0/1"> + <sbol:access rdf:resource="http://sbols.org/v2#public"/> + <sbol:definition rdf:resource="http://synbiochem.co.uk/ComponentDefinition/P21684_20000_gene/1"/> + <sbol:displayId>P21684_20000_gene_0</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/P21684_20000_gene_0"/> + <sbol:roleIntegration rdf:resource="http://sbols.org/v2#mergeRoles"/> + <sbol:version>1</sbol:version> + </sbol:Component> + </sbol:component> + <sbol:displayId>plasmid30</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000155"/> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_0/1"> + <sbol:displayId>constraint_0</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/BBa_I13800_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_0"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/BBa_I50041_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_1/1"> + <sbol:displayId>constraint_1</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/BBa_K1847014_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_1"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/BBa_I13800_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_2/1"> + <sbol:displayId>constraint_2</sbol:displayId> + <sbol:object rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/P21684_20000_gene_0/1"/> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_2"/> + <sbol:restriction rdf:resource="http://sbols.org/v2#precedes"/> + <sbol:subject rdf:resource="http://synbiochem.co.uk/ComponentDefinition/plasmid30/BBa_K1847014_0/1"/> + <sbol:version>1</sbol:version> + </sbol:SequenceConstraint> + </sbol:sequenceConstraint> + <sbol:sequenceConstraint> + <sbol:SequenceConstraint rdf:about="http://synbiochem.co.uk/ComponentDefinition/plasmid30/constraint_3/1"> + 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rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_1_3_prime_assembly/1"> + <dcterms:title>rxn_1_3_prime_assembly</dcterms:title> + <sbol:displayId>rxn_1_3_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_1_3_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_1_3_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_1_3_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_1_5_prime_assembly/1"> + <dcterms:title>rxn_1_5_prime_assembly</dcterms:title> + <sbol:displayId>rxn_1_5_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_1_5_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_1_5_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_1_5_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_2_3_prime_assembly/1"> + <dcterms:title>rxn_2_3_prime_assembly</dcterms:title> + <sbol:displayId>rxn_2_3_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_2_3_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_2_3_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_2_3_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_2_5_prime_assembly/1"> + <dcterms:title>rxn_2_5_prime_assembly</dcterms:title> + <sbol:displayId>rxn_2_5_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_2_5_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_2_5_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_2_5_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_3_3_prime_assembly/1"> + <dcterms:title>rxn_3_3_prime_assembly</dcterms:title> + <sbol:displayId>rxn_3_3_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_3_3_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_3_3_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_3_3_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="http://synbiochem.co.uk/ComponentDefinition/rxn_3_5_prime_assembly/1"> + <dcterms:title>rxn_3_5_prime_assembly</dcterms:title> + <sbol:displayId>rxn_3_5_prime_assembly</sbol:displayId> + <sbol:persistentIdentity rdf:resource="http://synbiochem.co.uk/ComponentDefinition/rxn_3_5_prime_assembly"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000143"/> + <sbol:sequence rdf:resource="http://examples.org/Sequence/rxn_3_5_prime_assembly_seq/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://liverpool.ac.uk/ComponentDefinition/rxn_3_5_prime_assembly/1"/> + </sbol:ComponentDefinition> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_B1006/1"> + <dc:creator>Haiyao Huang</dc:creator> + <dcterms:created>2006-08-30T11:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_B1006/1</dcterms:description> + <dcterms:modified>2015-08-31T04:07:21Z</dcterms:modified> + <dcterms:title>Ter</dcterms:title> + <sbol:displayId>BBa_B1006</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000141"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/Terminator"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_B1006_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/1"> + <dcterms:title>B1006</dcterms:title> + <sbol:displayId>annotation1898428</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1"> + <sbol:displayId>range1898428</sbol:displayId> + <sbol:end>39</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428"/> + <sbol:start>1</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898428"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/BioBrick"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/1"> + <dcterms:title>modified thr terminator</dcterms:title> + <sbol:displayId>annotation1898429</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1"> + <sbol:displayId>range1898429</sbol:displayId> + <sbol:end>31</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429"/> + <sbol:start>10</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898429"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000313"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/stem_loop"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/1"> + <dcterms:title>PolyA</dcterms:title> + <sbol:displayId>annotation1898430</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1"> + <sbol:displayId>range1898430</sbol:displayId> + <sbol:end>39</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430"/> + <sbol:start>32</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898430"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000553"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/polya"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/1"> + <dcterms:title>PolyA</dcterms:title> + <sbol:displayId>annotation1898431</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1"> + <sbol:displayId>range1898431</sbol:displayId> + <sbol:end>9</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431"/> + <sbol:start>1</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006/annotation1898431"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000553"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/polya"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>true</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/> + <igem:group_u_list>_41_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>745</igem:owner_id> + <igem:owning_group_id>41</igem:owning_group_id> + <igem:partStatus>Released HQ 2013</igem:partStatus> + <igem:sampleStatus>In stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Available"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>Artificial terminator, estimated %T~>90% +*8bp stem, 6nt loop +*Bidirectional, estimated reverse %T~>90%</sbh:mutableDescription> + <sbh:mutableNotes>Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.</sbh:mutableNotes> + <sbh:mutableProvenance>modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs</sbh:mutableProvenance> + <sbh:star>true</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_B1006"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_B1006_sequence/1"> + <sbol:displayId>BBa_B1006_sequence</sbol:displayId> + <sbol:elements>aaaaaaaaaccccgcccctgacagggcggggtttttttt</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_B1006_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_B1006"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_I13800/1"> + <dc:creator>robertb</dc:creator> + <dcterms:created>2004-07-18T11:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_I13800/1</dcterms:description> + <dcterms:modified>2015-08-31T04:07:36Z</dcterms:modified> + <dcterms:title>res1</dcterms:title> + <sbol:displayId>BBa_I13800</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I13800"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000316"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_I13800_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I13800/annotation902300/1"> + <dcterms:title>Ampicillin Resistance</dcterms:title> + <sbol:displayId>annotation902300</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I13800/annotation902300/range902300/1"> + <sbol:displayId>range902300</sbol:displayId> + <sbol:end>861</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/annotation902300/range902300"/> + <sbol:start>1</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I13800/annotation902300"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/misc"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/None"/> + <igem:group_u_list>_6_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>159</igem:owner_id> + <igem:owning_group_id>7</igem:owning_group_id> + <igem:sampleStatus>Not in stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Unavailable"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>This is the wild-type cassette for ampicillin resistance.</sbh:mutableDescription> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I13800"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_I13800_sequence/1"> + <sbol:displayId>BBa_I13800_sequence</sbol:displayId> + <sbol:elements>ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I13800_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I13800"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_I50032/1"> + <dc:creator>Reshma Shetty</dc:creator> + <dcterms:created>2007-04-10T11:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_I50032/1</dcterms:description> + <dcterms:modified>2015-08-31T04:07:42Z</dcterms:modified> + <dcterms:title>p15A</dcterms:title> + <sbol:displayId>BBa_I50032</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000296"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/DNA"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_I50032_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/1"> + <dcterms:title>p15A origin of replication</dcterms:title> + <sbol:displayId>annotation1928307</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307/1"> + <sbol:displayId>range1928307</sbol:displayId> + <sbol:end>1223</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/annotation1928307/range1928307"/> + <sbol:start>399</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/annotation1928307"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/misc"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/1"> + <dcterms:title>repeat region</dcterms:title> + <sbol:displayId>annotation1928308</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308/1"> + <sbol:displayId>range1928308</sbol:displayId> + <sbol:end>1450</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/annotation1928308/range1928308"/> + <sbol:start>1223</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032/annotation1928308"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/misc"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/Works"/> + <igem:group_u_list>_41_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>126</igem:owner_id> + <igem:owning_group_id>70</igem:owning_group_id> + <igem:sampleStatus>Not in stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Unavailable"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>p15A origin of replication which is supposed to have low to medium copy number. It is also the origin of replication in pSB3* series plasmids.</sbh:mutableDescription> + <sbh:mutableNotes>The boundaries of this part are quite liberal to ensure that this part is a functional origin.</sbh:mutableNotes> + <sbh:mutableProvenance>This part was constructed by PCR amplification of pSB3K3-P1010.</sbh:mutableProvenance> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I50032"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_I50032_sequence/1"> + <sbol:displayId>BBa_I50032_sequence</sbol:displayId> + <sbol:elements>atggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50032_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I50032"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_I50041/1"> + <dc:creator>Reshma Shetty</dc:creator> + <dcterms:created>2006-03-13T12:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_I50041/1</dcterms:description> + <dcterms:modified>2015-08-31T04:07:42Z</dcterms:modified> + <dcterms:title>BBR1</dcterms:title> + <sbol:displayId>BBa_I50041</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000296"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_I50041_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/1"> + <dcterms:title>putative polyA</dcterms:title> + <sbol:displayId>annotation1932215</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215/1"> + <sbol:displayId>range1932215</sbol:displayId> + <sbol:end>91</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932215/range1932215"/> + <sbol:start>76</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932215"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000553"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/polya"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/1"> + <dcterms:title>repA</dcterms:title> + <sbol:displayId>annotation1932216</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216/1"> + <sbol:displayId>range1932216</sbol:displayId> + <sbol:end>1152</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932216/range1932216"/> + <sbol:start>202</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932216"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000316"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/cds"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/1"> + <dcterms:title>inverted repeat</dcterms:title> + <sbol:displayId>annotation1932217</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217/1"> + <sbol:displayId>range1932217</sbol:displayId> + <sbol:end>1176</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932217/range1932217"/> + <sbol:start>1154</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932217"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/misc"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/1"> + <dcterms:title>RBS</dcterms:title> + <sbol:displayId>annotation1932218</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218/1"> + <sbol:displayId>range1932218</sbol:displayId> + <sbol:end>1159</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932218/range1932218"/> + <sbol:start>1154</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932218"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000139"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/rbs"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/1"> + <dcterms:title>RBS</dcterms:title> + <sbol:displayId>annotation1932219</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219/1"> + <sbol:displayId>range1932219</sbol:displayId> + <sbol:end>1168</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932219/range1932219"/> + <sbol:start>1156</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932219"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000139"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/rbs"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/1"> + <dcterms:title>promoter region</dcterms:title> + <sbol:displayId>annotation1932220</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220/1"> + <sbol:displayId>range1932220</sbol:displayId> + <sbol:end>1192</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932220/range1932220"/> + <sbol:start>1173</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932220"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/promoter"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/1"> + <dcterms:title>inverted repeat</dcterms:title> + <sbol:displayId>annotation1932221</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221/1"> + <sbol:displayId>range1932221</sbol:displayId> + <sbol:end>1200</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932221/range1932221"/> + <sbol:start>1179</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932221"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000110"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/misc"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/1"> + <dcterms:title>rep promoter -10</dcterms:title> + <sbol:displayId>annotation1932222</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222/1"> + <sbol:displayId>range1932222</sbol:displayId> + <sbol:end>1188</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932222/range1932222"/> + <sbol:start>1183</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932222"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/promoter"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/1"> + <dcterms:title>rep promoter -35</dcterms:title> + <sbol:displayId>annotation1932223</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223/1"> + <sbol:displayId>range1932223</sbol:displayId> + <sbol:end>1210</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932223/range1932223"/> + <sbol:start>1205</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932223"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/promoter"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/1"> + <dcterms:title>Mutated SpeI site</dcterms:title> + <sbol:displayId>annotation1932224</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224/1"> + <sbol:displayId>range1932224</sbol:displayId> + <sbol:end>1224</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932224/range1932224"/> + <sbol:start>1219</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932224"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0001059"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/mutation"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/1"> + <dcterms:title>direct repeat</dcterms:title> + <sbol:displayId>annotation1932225</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225/1"> + <sbol:displayId>range1932225</sbol:displayId> + <sbol:end>1269</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041/annotation1932225/range1932225"/> + <sbol:start>1250</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + 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rdf:resource="http://identifiers.org/so/SO:0001059"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/mutation"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/None"/> + <igem:group_u_list>_41_6_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>126</igem:owner_id> + <igem:owning_group_id>45</igem:owning_group_id> + <igem:sampleStatus>Not in stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Unavailable"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040.</sbh:mutableDescription> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I50041"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_I50041_sequence/1"> + <sbol:displayId>BBa_I50041_sequence</sbol:displayId> + <sbol:elements>gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_I50041_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_I50041"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_J56012/1"> + <dc:creator>Matt Eames</dc:creator> + <dcterms:created>2006-10-31T12:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_J56012/1</dcterms:description> + <dcterms:modified>2015-08-31T03:22:59Z</dcterms:modified> + <dcterms:title>Ptrc</dcterms:title> + <sbol:displayId>BBa_J56012</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J56012"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_J56012_sequence/1"/> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:group_u_list>_79_98_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>1132</igem:owner_id> + <igem:owning_group_id>98</igem:owning_group_id> + <igem:sampleStatus>Not in stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Unavailable"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>Seuqence is inverted by the FimE protein. Inside recognition sequence is a Ptrc promoter sequence. Switch is irreversible.</sbh:mutableDescription> + <sbh:mutableNotes>None</sbh:mutableNotes> + <sbh:mutableProvenance>Synthesized by DNA 2.0</sbh:mutableProvenance> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J56012"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_J56012_sequence/1"> + <sbol:displayId>BBa_J56012_sequence</sbol:displayId> + <sbol:elements>ctgccaacactgcacagttttcccccaaaagatgaaacatttggggccaaactgtccatattatacgagccggatgattaattgtcaaccataaaaatattaaaaaacgacaaaaagcatctaactgtttgatatgtaaattatttctattgtaaattaatttcacatcacctccgctatatgtaaagctaacgtttctgtggctcgacgcatcttcctcattcttctctccaaaaaccacctcatgcaatataaacatctataaataaagataacaatagaatattaagccaacaaataaactgaaaaagtttgtccgcgatgctttcctctatgagtcaaaatggccccaattgtcttgtatttatttgtttttttaactttattatcaataagttaaatcggtacc</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J56012_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J56012"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_J64101/1"> + <dc:creator>Jeffrey J Tabor</dc:creator> + <dcterms:created>2007-04-13T11:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_J64101/1</dcterms:description> + <dcterms:modified>2015-05-08T01:08:17Z</dcterms:modified> + <dcterms:title>ColE1</dcterms:title> + <sbol:displayId>BBa_J64101</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000296"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#partType/Plasmid"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_J64101_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931320/1"> + <dcterms:title>BBa_J64100</dcterms:title> + <sbol:displayId>annotation1931320</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931320/range1931320/1"> + <sbol:displayId>range1931320</sbol:displayId> + <sbol:end>2800</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931320/range1931320"/> + <sbol:start>1</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931320"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/BioBrick"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931321/1"> + <dcterms:title>G00100</dcterms:title> + <sbol:displayId>annotation1931321</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931321/range1931321/1"> + <sbol:displayId>range1931321</sbol:displayId> + <sbol:end>25</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931321/range1931321"/> + <sbol:start>6</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931321"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0005850"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/primer_binding"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931322/1"> + <dcterms:title>prefix</dcterms:title> + <sbol:displayId>annotation1931322</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931322/range1931322/1"> + <sbol:displayId>range1931322</sbol:displayId> + <sbol:end>145</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931322/range1931322"/> + <sbol:start>124</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931322"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/BioBrick"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931323/1"> + <dcterms:title>B0025</dcterms:title> + <sbol:displayId>annotation1931323</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931323/range1931323/1"> + <sbol:displayId>range1931323</sbol:displayId> + <sbol:end>274</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931323/range1931323"/> + <sbol:start>146</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931323"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/BioBrick"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931324/1"> + <dcterms:title>suffix</dcterms:title> + <sbol:displayId>annotation1931324</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931324/range1931324/1"> + <sbol:displayId>range1931324</sbol:displayId> + <sbol:end>295</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931324/range1931324"/> + <sbol:start>275</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931324"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000804"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/BioBrick"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931325/1"> + <dcterms:title>G00101</dcterms:title> + <sbol:displayId>annotation1931325</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931325/range1931325/1"> + <sbol:displayId>range1931325</sbol:displayId> + <sbol:end>450</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931325/range1931325"/> + <sbol:start>431</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931325"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0005850"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/primer_binding"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931326/1"> + <dcterms:title>G00101</dcterms:title> + <sbol:displayId>annotation1931326</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_J64101/annotation1931326/range1931326/1"> + <sbol:displayId>range1931326</sbol:displayId> + <sbol:end>502</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931326/range1931326"/> + <sbol:start>483</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/reverse"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101/annotation1931326"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0005850"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/primer_binding"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/None"/> + <igem:group_u_list>_98_</igem:group_u_list> + <igem:m_user_id>0</igem:m_user_id> + <igem:owner_id>88</igem:owner_id> + <igem:owning_group_id>98</igem:owning_group_id> + <igem:sampleStatus>Not in stock</igem:sampleStatus> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Unavailable"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>ColE1 biobrick plasmid with backward terminator in MCS</sbh:mutableDescription> + <sbh:mutableNotes>None</sbh:mutableNotes> + <sbh:mutableProvenance>Composite registry part derived from J64100 and B0025</sbh:mutableProvenance> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J64101"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_J64101_sequence/1"> + <sbol:displayId>BBa_J64101_sequence</sbol:displayId> + <sbol:elements>tcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatcctagggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgacttgtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatcgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcacc</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_J64101_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_J64101"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <sbol:ComponentDefinition rdf:about="https://synbiohub.org/public/igem/BBa_K1847014/1"> + <dc:creator>Anna Fomitcheva, Lisa Baumgartner</dc:creator> + <dcterms:created>2015-08-25T11:00:00Z</dcterms:created> + <dcterms:description>https://synbiohub.org/public/igem/BBa_K1847014/1</dcterms:description> + <dcterms:modified>2015-08-26T09:07:45Z</dcterms:modified> + <dcterms:title>PlacUV5</dcterms:title> + <sbol:displayId>BBa_K1847014</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:sequence rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014_sequence/1"/> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438101/1"> + <dcterms:title>plac UV5</dcterms:title> + <sbol:displayId>annotation2438101</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438101/range2438101/1"> + <sbol:displayId>range2438101</sbol:displayId> + <sbol:end>31</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438101/range2438101"/> + <sbol:start>1</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438101"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000167"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/promoter"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:sequenceAnnotation> + <sbol:SequenceAnnotation rdf:about="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438102/1"> + <dcterms:title>lacO</dcterms:title> + <sbol:displayId>annotation2438102</sbol:displayId> + <sbol:location> + <sbol:Range rdf:about="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438102/range2438102/1"> + <sbol:displayId>range2438102</sbol:displayId> + <sbol:end>55</sbol:end> + <sbol:orientation rdf:resource="http://sbols.org/v2#inline"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438102/range2438102"/> + <sbol:start>39</sbol:start> + <sbol:version>1</sbol:version> + <igem:direction rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#direction/forward"/> + </sbol:Range> + </sbol:location> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014/annotation2438102"/> + <sbol:role rdf:resource="http://identifiers.org/so/SO:0000057"/> + <sbol:role rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#feature/operator"/> + <sbol:version>1</sbol:version> + </sbol:SequenceAnnotation> + </sbol:sequenceAnnotation> + <sbol:type rdf:resource="http://www.biopax.org/release/biopax-level3.owl#DnaRegion"/> + <sbol:version>1</sbol:version> + <igem:discontinued>false</igem:discontinued> + <igem:dominant>false</igem:dominant> + <igem:experience rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#experience/None"/> + <igem:group_u_list>_2273_</igem:group_u_list> + <igem:m_user_id>26442</igem:m_user_id> + <igem:owner_id>26442</igem:owner_id> + <igem:owning_group_id>9</igem:owning_group_id> + <igem:status rdf:resource="http://wiki.synbiohub.org/wiki/Terms/igem#status/Planning"/> + <sbh:bookmark>false</sbh:bookmark> + <sbh:mutableDescription>Promoter regulated by LacI.</sbh:mutableDescription> + <sbh:mutableProvenance>Synthetic synthesis.</sbh:mutableProvenance> + <sbh:star>false</sbh:star> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_K1847014"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:ComponentDefinition> + <sbol:Sequence rdf:about="https://synbiohub.org/public/igem/BBa_K1847014_sequence/1"> + <sbol:displayId>BBa_K1847014_sequence</sbol:displayId> + <sbol:elements>tttacactttatgcttccggctcgtataatgtgtggaattgtgagcggataacaa</sbol:elements> + <sbol:encoding rdf:resource="http://www.chem.qmul.ac.uk/iubmb/misc/naseq.html"/> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/BBa_K1847014_sequence"/> + <sbol:version>1</sbol:version> + <prov:wasDerivedFrom rdf:resource="http://parts.igem.org/Part:BBa_K1847014"/> + <prov:wasGeneratedBy rdf:resource="https://synbiohub.org/public/igem/igem2sbol/1"/> + </sbol:Sequence> + <prov:Activity rdf:about="https://synbiohub.org/public/igem/igem2sbol/1"> + <dc:creator>Chris J. Myers</dc:creator> + <dc:creator>James Alastair McLaughlin</dc:creator> + <dcterms:description>Conversion of the iGEM parts registry to SBOL2.1</dcterms:description> + <dcterms:title>iGEM to SBOL conversion</dcterms:title> + <sbol:displayId>igem2sbol</sbol:displayId> + <sbol:persistentIdentity rdf:resource="https://synbiohub.org/public/igem/igem2sbol"/> + <sbol:version>1</sbol:version> + <prov:endedAtTime>2017-03-06T15:00:00.000Z</prov:endedAtTime> + </prov:Activity> +</rdf:RDF>