annotate fasta_to_tabular.xml @ 2:091edad7622f draft

"planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/fasta_to_tabular commit cd1ed08574b749eee2a3f6e6151dbb0c8ca15bbf"

<tool id="fasta2tab" name="FASTA-to-Tabular" version="1.1.1" profile="16.04">
    <description>converter</description>
    <requirements>
        <requirement type="package" version="3.7">python</requirement>
    </requirements>
    <command>
python '$__tool_directory__/fasta_to_tabular.py' '$input' '$output' $keep_first $descr_columns
    </command>
    <inputs>
        <param name="input" type="data" format="fasta" label="Convert these sequences"/>
        <param name="descr_columns" type="integer" value="1" min="1" label="How many columns to divide title string into?" help="Typically 2 to take the ID (first word) and decription (rest) as two columns, or 1 to give a single column">
        </param>
        <param name="keep_first" type="integer" value="0" min="0" label="How many title characters to keep?" help="Applies only to the first column taken from the title string ('0' = keep the whole thing), useful when your sequence identifiers are all the same length.">
        </param>
    </inputs>
    <outputs>
        <data name="output" format="tabular"/>
    </outputs>
    <tests>
        <test>
            <param name="input" value="454.fasta" />
            <param name="descr_columns" value="1"/>
            <param name="keep_first" value="0"/>
            <output name="output" file="fasta_to_tabular_out1.tabular" />
        </test>

        <test>
            <param name="input" value="4.fasta" />
            <param name="descr_columns" value="1"/>
            <param name="keep_first" value="0"/>
            <output name="output" file="fasta_to_tabular_out2.tabular" />
        </test>

        <test>
            <param name="input" value="454.fasta" />
            <param name="descr_columns" value="1"/>
            <param name="keep_first" value="14"/>
            <output name="output" file="fasta_to_tabular_out3.tabular" />
        </test>

        <test>
            <param name="input" value="454.fasta" />
            <param name="descr_columns" value="2"/>
            <param name="keep_first" value="0"/>
            <output name="output" file="fasta_to_tabular_out4.tabular" />
        </test>

        <test>
            <param name="input" value="454.fasta" />
            <param name="descr_columns" value="5"/>
            <param name="keep_first" value="0"/>
            <output name="output" file="fasta_to_tabular_out5.tabular" />
        </test>

        <test>
            <param name="input" value="454.fasta" />
            <param name="descr_columns" value="5"/>
            <param name="keep_first" value="10"/>
            <output name="output" file="fasta_to_tabular_out6.tabular" />
        </test>

    </tests>
    <help><![CDATA[

**What it does**

This tool converts FASTA formatted sequences to TAB-delimited format.

Many tools consider the first word of the FASTA "&gt;" title line to be an identifier, and any remaining text to be a free form description.
It is therefore useful to split this text into two columns in Galaxy (identifier and any description) by setting **How many columns to divide title string into?** to **2**.
In some cases the description can be usefully broken up into more columns -- see the examples .

The option *How many characters to keep?* allows to select a specified number of letters from the beginning of each FASTA entry.
With the introduction of the **How many columns to divide title string into?** option this setting is of limited use, but does still allow you to truncate the identifier.

-----

**Example**

Suppose you have the following FASTA formatted sequences from a Roche (454) FLX sequencing run::

    >EYKX4VC02EQLO5 length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_
    TCCGCGCCGAGCATGCCCATCTTGGATTCCGGCGCGATGACCATCGCCCGCTCCACCACG
    TTCGGCCGGCCCTTCTCGTCGAGGAATGACACCAGCGCTTCGCCCACG
    >EYKX4VC02D4GS2 length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_
    AATAAAACTAAATCAGCAAAGACTGGCAAATACTCACAGGCTTATACAATACAAATGTAA

Running this tool with the default settings will produce this (2 column output):

========================================================================== =======================================
EYKX4VC02EQLO5 length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_ TCCGCGCCGAGCATGCCCATCTTGGATTCCGGC...ACG
EYKX4VC02D4GS2 length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_  AATAAAACTAAATCAGCAAAGACTGGCAAATAC...TAA
========================================================================== =======================================

Having the full title line (the FASTA "&gt;" line text) as a column is not always ideal.

The **How many characters to keep?** option is useful if your identifiers are all the same length.
In this example the identifier is 14 characters, so setting **How many characters to keep?** to **14** (and leaving **How many columns to divide title string into?** as the default, **1**) will produce this (2 column output):

============== =======================================
EYKX4VC02EQLO5 TCCGCGCCGAGCATGCCCATCTTGGATTCCGGC...ACG
EYKX4VC02D4GS2 AATAAAACTAAATCAGCAAAGACTGGCAAATAC...TAA
============== =======================================

If however your FASTA file has identifiers of variable length, it is better to split the text into at least two columns.
Running this tool with **How many columns to divide title string into?** to **2** will produce this (3 column output):

============== =========================================================== =======================================
EYKX4VC02EQLO5 length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_ TCCGCGCCGAGCATGCCCATCTTGGATTCCGGC...ACG
EYKX4VC02D4GS2 length=60 xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_  AATAAAACTAAATCAGCAAAGACTGGCAAATAC...TAA
============== =========================================================== =======================================

Running this tool with **How many columns to divide title string into?** to **5** will produce this (5 column output):

============== ========== ============ ======== ========================== =======================================
EYKX4VC02EQLO5 length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_ TCCGCGCCGAGCATGCCCATCTTGGATTCCGGC...ACG
EYKX4VC02D4GS2 length=60  xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_ AATAAAACTAAATCAGCAAAGACTGGCAAATAC...TAA
============== ========== ============ ======== ========================== =======================================

Running this tool with **How many columns to divide title string into?** to **5** and **How many characters to keep?** to **10** will produce this (5 column output).
Notice that only the first column is truncated to 10 characters -- and be careful not to trim your sequence names too much (generally they should be unique):

========== ========== ============ ======== ========================== =======================================
EYKX4VC02E length=108 xy=1826_0455 region=2 run=R_2007_11_07_16_15_57_ TCCGCGCCGAGCATGCCCATCTTGGATTCCGGC...ACG
EYKX4VC02D length=60  xy=1573_3972 region=2 run=R_2007_11_07_16_15_57_ AATAAAACTAAATCAGCAAAGACTGGCAAATAC...TAA
========== ========== ============ ======== ========================== =======================================

Note the sequences have been truncated for display purposes in the above tables.

    ]]></help>
</tool>